Qualitative and quantitative sampling device for in-situ dormant egg bank germination of water body and water pollution monitor

Abstract

The invention relates to a water in-situ dormant egg bank germination qualitative and quantitative sampling device and a water pollution monitor, the device comprises a water surface assembly and a water bottom assembly, the water surface assembly comprises a floating body and a sampling pipe, and the water bottom assembly comprises a long cylinder, a fixed ring, a sliding ring, a first spring, a balancing weight and a retractable isolation mechanism; the retractable isolation mechanism comprises a flexible isolation cover and a plurality of folding connecting rods, wherein the folding connecting rods are evenly hinged in the circumferential direction of the sliding ring at intervals through the upper ends of the folding connecting rods, and the folding connecting rods are used for retracting, folding or releasing and unfolding the isolation cover. The device has the advantages that the device has a contraction folding state and a release unfolding state, the device is convenient to store after being contracted and folded, the device can be conveniently released and unfolded in a correct posture after being put into a water body, a required sampling isolation area is formed on the water bottom ground after being released and unfolded, and the total amount of dormant eggs in a dormant egg bank in the isolation area is relatively stable; the influence of external dormant eggs on a research area is avoided, and in-situ qualitative and quantitative research on dormant egg bank germination is facilitated.

Description

Technical Field

[0001] The present invention relates to the fields of water ecological environment protection and scientific research, and particularly relates to an in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies and a water pollution monitor. Background Art

[0002] Many aquatic organisms such as bacteria (e.g., Bacillus, Clostridium, Actinomycetes), fungi (e.g., Oomycetes, Chytridiomycetes, Ascomycetes), zooplankton (e.g., protozoa, rotifers, cladocerans, copepods), and aquatic insects (e.g., chironomids, mayflies, dragonflies) will produce dormant bodies at a certain stage in their life cycles. According to different sources above, the dormant bodies can be subdivided into endospores, cysts, spores, eggs, etc., which are collectively referred to as dormant eggs for convenience of description hereinafter.

[0003] Different from living organisms, dormant eggs are generally coated with complex protective structures on the outside and their metabolic activities are extremely reduced, so they can survive for months, years, or even decades. Most dormant eggs do not hatch immediately, but sink to the bottom and become part of the sediment, and form a dormant egg bank over time. The dormant egg bank has strong resistance to harsh environmental conditions (such as high temperature, low temperature, hypoxia, drought, acid-base, ultraviolet rays, drastic changes in osmotic pressure, etc.), and is also an important way for the preservation, overwintering, and spread of aquatic organisms.

[0004] The resuscitation of dormant eggs has a direct impact on the structure of the living community in the water column (such as species types, numbers, and relative abundances), and the regular sequential resuscitation of dormant eggs (such as phenology) also maintains the genetic diversity of populations and communities. For example, the staggered resuscitation of dormant eggs with different genotypes within a species can effectively alleviate intraspecific competition, optimize resource utilization, maintain genetic diversity, and regulate population dynamics. Similarly, the staggered resuscitation of dormant eggs of different species also promotes the seasonal succession of communities and the sympatric coexistence of species with similar ecological niches. From the perspective of the ecosystem, the regular resuscitation of dormant eggs also promotes the upward transfer of matter and energy along the food web, maintaining the stability of the ecosystem structure and function. In addition, the hatching situation of the dormant egg bank can also intuitively and effectively reflect the impacts of climate change and human activities on the water ecosystem. For example, climate warming and the urban heat island effect will significantly change the germination pattern of dormant eggs; the change in underwater light intensity caused by the change in water turbidity will also affect the germination of dormant eggs; heavy metals (such as cadmium, copper, etc.) and organic pollutants (such as polycyclic aromatic hydrocarbons, environmental hormones, etc.) show toxic effects on dormant eggs. In summary, on the one hand, the dormant egg bank has important theoretical and application values in maintaining biodiversity, recording community evolution, and regulating ecosystem functions; on the other hand, the impact of climate change and human activities on the water ecosystem can also be reflected by monitoring the hatching status of the dormant egg bank.

[0005] However, there is currently no good method to qualitatively and quantitatively study the germination dynamics of the resting egg bank. For this reason, the present invention designs a sample collection device for in-situ qualitative and quantitative monitoring of the resuscitation of the resting egg bank in water bodies and a water pollution monitor based on it to address the pain points in current production and scientific research. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to provide a device for qualitatively and quantitatively sampling the germination of in-situ resting egg banks in water bodies and a water pollution monitor, aiming to overcome the above deficiencies existing in the prior art.

[0007] The technical solution of the present invention to solve the above technical problem is as follows: A device for qualitatively and quantitatively sampling the germination of in-situ resting egg banks in water bodies, which includes a water surface component and a water bottom component. The water surface component includes a floating body and a sampling tube. The water bottom component includes a long cylinder, a fixed ring, a sliding ring, a first spring, a counterweight, and a retractable isolation mechanism. The long cylinder is vertically arranged. The fixed ring is sleeved and fixed on the outer side wall of the top of the long cylinder. The sliding ring is slidably sleeved on the long cylinder and is located below the fixed ring. The first spring is clamped between the fixed ring and the sliding ring. The retractable isolation mechanism includes a flexible isolation cover and a plurality of folding connecting rods evenly spaced and hinged at the upper ends in the circumferential direction of the sliding ring. The lower ends of the folding connecting rods are hinged to the counterweight. The folding connecting rods are used to contract and fold or expand and unfold the isolation cover. After the isolation cover is contracted and folded, it approaches and adheres to the outer wall of the long cylinder. After the isolation cover is expanded and unfolded, it forms an upper-small-lower-large isolation area covering the bottom ground of the water body. The floating body is connected to the long cylinder by a pull rope. The lower end of the sampling tube is located underwater and communicates with the isolation area. The upper end of the sampling tube is located above the water surface and is detachably connected to the floating body.

[0008] On the basis of the above technical solution, the present invention can be further improved as follows.

[0009] Further, each of the folding connecting rods includes an upper connecting rod and a lower connecting rod hinged end to end. The upper end of the upper connecting rod is hinged to the sliding ring. The lower end of the lower connecting rod is hinged to the counterweight. The side wall of the isolation cover is fixedly connected to each of the upper connecting rods. The upper end of the isolation cover is fixedly connected to the periphery of the sliding ring and extends to the hinged part of the upper connecting rod and the lower connecting rod. A torsion spring is provided at the end-to-end hinged part of the lower end of the upper connecting rod and the upper end of the lower connecting rod.

[0010] The advantages of the above further improvement are as follows. The folding link is formed by the upper link and the lower link being end-to-end hinged. When the sliding ring is pushed downward by the first spring, the upper link and the lower link can move away from the long cylinder body at the hinged part, so that the isolation cover can be smoothly and steadily opened. The folding link consists of only two levels of links, namely the upper link and the lower link. The force transmission and the movement relationship between the upper and lower links are relatively clear and controllable. The structure is relatively simple and the failure rate is low. The setting of the torsion spring can ensure that there is always a tendency force for the upper and lower links to move away from the long cylinder body at the hinged part. As long as the sliding ring moves downward, the upper and lower links will move along the set path, without reverse movement and without generating a large supporting force in the vertical direction, which will affect the realization of the first trigger being pressed to trigger the locking rod to unlock the sliding ring in the following text.

[0011] Further, limiting holes are radially symmetrically formed on the cylinder wall of the long cylinder body. A reset rod, a reset slider, a locking rod and a first trigger are arranged in the long cylinder body. The upper end of the reset rod is fixedly connected to the midpoint of the horizontally arranged locking rod, and the lower end is fixedly connected to the reset slider. The reset slider is slidably matched with the inner wall of the long cylinder body. The reset slider is fixedly connected to the top surface of the counterweight block through the first trigger. Retractable ball plungers are arranged at both ends of the locking rod. After the isolation cover is shrunk and folded, the two ball plungers protrude from the corresponding limiting holes and block and limit the sliding ring from below. When the first trigger is triggered, it can push the reset slider upward and retract the ball plungers into the limiting holes to release the limitation on the sliding ring.

[0012] The advantages of the above further improvement are as follows. Through the setting of the reset rod, the reset slider, the locking rod and the first trigger, the maintainance of the retractable isolation mechanism in the shrunk and folded state and the expanded state and the switching between the two states are realized. The triggering of the first trigger means that the first trigger gives an upward pushing force to the reset slider. This force can be actively generated by the first trigger itself, or it can be the reaction force generated by the first trigger on the reset slider due to the long cylinder body pressing the first trigger due to its own weight.

[0013] Further, a cavity for installing a second spring is arranged in the locking rod. Through holes communicating with the cavity are formed at both ends of the locking rod. The tail of the ball plunger is located in the cavity, and the head protrudes outwards from the through hole. The tails of the two ball plungers squeeze the second spring from both ends. The first trigger is a reset spring. When the long cylinder body falls vertically to the bottom of the water and the counterweight block touches the bottom ground, the self-weight of the long cylinder body will squeeze the reset spring and push the reset slider upward.

[0014] The advantages of the above further improvement are as follows. The retractable ball plunger structure with the above structure is relatively simple, and can effectively realize that when the reset rod moves upward, the ball plunger is automatically retracted under pressure and retracts from the limit hole into the long cylinder body to release the unlocking effect on the sliding ring limit. At the same time, when the reset rod does not move upward, the downward pressure of the sliding ring on the ball plunger cannot retract the ball plunger, realizing the blocking and limiting effect on the sliding ring.

[0015] Furthermore, it further includes a second trigger member disposed in the long cylinder body. The second trigger member includes an electric telescopic mechanism that can drive the locking rod to move up and down. The inner wall of the long cylinder body above the limit hole is provided with a ball guide groove for guiding the end of the ball plunger.

[0016] The advantages of the above further improvement are that the controllability of the locking and unlocking of the sliding ring by the locking rod is better and does not depend on the unlocking by the self-weight pressure of the long cylinder body, and it has better flexibility and a higher success rate when placing the underwater component.

[0017] Furthermore, a sampling valve and a sampling pump are provided on the sampling tube, and the sampling valve and the sampling pump are located above the water surface.

[0018] The advantages of the above further improvement are that sampling is more convenient through the setting of the sampling valve and the sampling pump, and there is no need for manual use of suction equipment for sampling.

[0019] Furthermore, there are four folding linkages, and the isolation cover is in the shape of a regular square pyramid after being unfolded.

[0020] The advantages of the above further improvement are that the area of the isolated area is easier to measure than that of a triangular pyramid, and at the same time, the overall stability is better.

[0021] Furthermore, a signal lamp and a signal flag are provided on the top of the floating body, and a battery for powering the signal lamp is provided inside the floating body.

[0022] The advantages of the above further improvement are that the signal lamp and the signal flag can play a good guiding role, which is convenient for quickly finding the target on the broad water surface during sampling.

[0023] Furthermore, a conduit is provided in the long cylinder body. The lower end of the conduit extends downward to the lower part of the long cylinder body and is communicated with the isolation area through a water intake hole on the cylinder wall. The upper end of the conduit passes through the upper end cover of the long cylinder body and is communicated with the lower end of the sampling tube, and an electric valve is provided at the water intake hole.

[0024] The advantages of the above further improvement are that the setting of the conduit facilitates the connection between the sampling tube and the isolation area through the long cylinder body, and simplifies the installation connection between the sampling tube and the long cylinder body.

[0025] The present invention also provides a water pollution monitor, which includes a fully automatic microbial identification and analysis device and the above-mentioned in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies provides the fully automatic microbial identification and analysis device with water samples collected in-situ.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] The retractable isolation mechanism of the in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies provided by the present invention has two states: contraction and folding, and expansion and unfolding. In the contraction and folding state, it is not only convenient to store when not in use, but also convenient for the device to be put into the bottom of the water body (with small resistance and no tipping or tilting) and ensure that it unfolds in the correct posture in the designated area on the bottom surface of the water body to form the required sampling isolation area that is properly shielded and isolated. When in the expansion and unfolding state, the water body in the designated area on the water surface and a certain space above it is isolated by the isolation cover and separated from other areas. The total amount of dormant eggs in the dormant egg bank in the isolation area is relatively stable, and dormant eggs that have a greater impact on the detection and research results will not be carried away or brought in by the external water flow. Therefore, in-situ sampling and detection research on the germination of the dormant egg bank can be realized to a certain extent, and qualitative and quantitative research and monitoring can be achieved, overcoming the deficiency in the prior art of lacking technical means for qualitative and quantitative research on the dormant egg bank.

[0028] The isolation area can greatly avoid the interference of the external environment on the total amount of dormant eggs during the research period. However, it does not isolate the environmental temperature, nor does it prohibit the material exchange between the chemical pollutants in the large environment water body and the water body in the isolation area through osmosis (through the sediment or the small gaps between the sediment and the periphery of the isolation cover). Therefore, the sampling and detection of the germination of the dormant egg bank in the isolation area can represent and relatively accurately reflect the overall germination situation of the dormant egg bank in the large environment water body, and can also reflect the water pollution situation, achieving the purpose of water pollution monitoring. Description of the Drawings

[0029] Figure 1 is a schematic diagram of the in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies provided by the present invention when the retractable isolation mechanism is in the contraction and folding state;

[0030] Figure 2 is Figure 1 a schematic diagram of the in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies shown when the retractable isolation mechanism is in the expansion and unfolding state;

[0031] Figure 3 is Figure 2 a top view of the in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies shown (the fixing ring and the sliding ring, etc. are not shown);

[0032] Figure 4 is Figure 2Top view of the sliding ring of the in-situ dormant egg bank germination qualitative and quantitative sampling device shown in the figure (the four black block areas in the figure represent the connecting parts hinged to the upper connecting rod);

[0033] Figure 5 is Figure 2 Schematic diagram of the reset rod, locking rod and ball plunger of the in-situ dormant egg bank germination qualitative and quantitative sampling device shown in the figure.

[0034] In the attached drawings, the list of components represented by each reference numeral is as follows:

[0035] 1. Floating body; 2. Long cylinder; 3. Fixed ring; 4. Sliding ring; 5. First spring; 6. Counterweight; 7. Isolation cover; 8. Pulling rope; 9. Upper connecting rod; 10. Lower connecting rod; 11. Torsion spring; 12. Reset rod; 13. Reset slider; 14. Locking rod; 15. Ball plunger; 16. Second spring; 17. Reset spring; 18. Signal flag; 19. Conduit; 20. Water intake hole. Specific implementation mode

[0036] The principles and features of the present invention will be described below in conjunction with the attached drawings and specific embodiments. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0037] In the description of the present invention, if terms indicating directions such as "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", etc. are used, the directions or positional relationships indicated are based on the directions or positional relationships shown in the attached drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be construed as a limitation to the present invention.

[0038] As Figures 1 to 5As shown in the figure, the present invention further provides an in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies, which includes a water surface component and a water bottom component. The water surface component includes a floating body 1 and a sampling tube. The water bottom component includes a long cylinder 2, a fixed ring 3, a sliding ring 4, a first spring 5, a counterweight 6 and a retractable isolation mechanism. The long cylinder 2 is vertically arranged. The fixed ring 3 is sleeved and fixed on the outer side wall of the top of the long cylinder 2. The sliding ring 4 is slidably sleeved on the long cylinder 2 and is located below the fixed ring 3. The first spring 5 is clamped between the fixed ring 3 and the sliding ring 4. The retractable isolation mechanism includes a flexible isolation cover 7 and a plurality of folding connecting rods evenly spaced and hinged at the upper ends in the circumferential direction of the sliding ring 4. The lower ends of the folding connecting rods are hinged to the counterweight 6. The folding connecting rods are used to contract and fold or unfold and expand the isolation cover 7. After the isolation cover 7 is contracted and folded, it approaches and adheres to the outer wall of the long cylinder 2. After the isolation cover 7 is unfolded and expanded, it forms an isolation area that is small at the top and large at the bottom covering the bottom ground of the water. The floating body 1 is connected to the long cylinder 2 by a pulling rope 8 (or the upper end of the pulling rope is connected to the floating body, the lower end is connected to the upper end of the long cylinder or passes through the long cylinder and is fixed to the counterweight below). The lower end of the sampling tube is located underwater and communicates with the isolation area. The upper end of the sampling tube is located above the water surface and is detachably connected to the floating body 1.

[0039] It should be noted that the first spring is sleeved on the long cylinder and is usually in a compressed state with a force to push the sliding ring downward; the floating body floats on the water surface, which not only plays a role in indicating the placement position of the entire device for subsequent searching and sampling, but also plays a role in pulling the long cylinder from above the water surface to better keep the long cylinder and the isolation cover in an upright state. The pulling rope is preferably made of a material with high strength and elasticity, and can keep the connecting rope in a relatively vertical state within a certain water level fluctuation range. The counterweight is preferably a stainless steel block or a lead block and has a relatively large weight to ensure that the entire device does not shift or tilt and overturn due to water flow impact or ebb and flow after the water bottom component is placed in place. The bottom area corresponding to the isolation area is preferably about 1 square meter after removing the area occupied by the counterweight (not exceeding 0.3 square meters). The isolation area should not be too large or too small.

[0040] In an embodiment of the present invention, as Figure 1 、 2 and shown in Figure 3, each of the folding connecting rods includes an upper connecting rod 9 and a lower connecting rod 10 that are end-to-end hinged. The upper end of the upper connecting rod 9 is hinged to the sliding ring 4. The lower end of the lower connecting rod 10 is hinged to the counterweight 6. The side wall of the isolation cover 7 is fixedly connected to each of the upper connecting rods 9. The upper end of the isolation cover 7 is fixedly connected to the periphery of the sliding ring 4, and the lower end extends to the hinge joint of the upper connecting rod 9 and the lower connecting rod 10. A torsion spring 11 is provided at the end-to-end hinge joint of the lower end of the upper connecting rod 9 and the upper end of the lower connecting rod 10.

[0041] It should be noted that the upper connecting rod and the lower connecting rod are preferably made of strong and corrosion-resistant metals such as stainless steel and titanium alloy, and can also be made of hard plastics such as ABS and PC. The isolation cover is a flexible, strong and corrosion-resistant plastic film or cloth, which is impermeable or can ensure that water molecules, chemicals, oxygen, etc. can penetrate but not penetrate the dormant eggs. The isolation cover is preferably a transparent film.

[0042] In one embodiment of the present invention, limiting holes are radially symmetrically provided on the wall of the long cylinder 2, and a reset rod 12, a reset slider 13, a locking rod 14 and a first trigger member are provided in the long cylinder 2. The upper end of the reset rod 12 is fixedly connected to the midpoint of the horizontally arranged locking rod 14, and the lower end is fixedly connected to the reset slider 13. The reset slider 13 is slidably matched with the inner wall of the long cylinder 2, and the reset slider 13 is fixedly connected to the top surface of the counterweight 6 through the first trigger member. Retractable ball plungers 15 are provided at both ends of the locking rod 14. After the isolation cover 7 is contracted and folded, the two ball plungers 15 extend from the corresponding limiting holes and block and limit the sliding ring 4 from below. When the first trigger member is triggered, the reset slider 13 can be pushed upward and the ball plunger 15 can be retracted into the limiting hole to release the limiting of the sliding ring 4.

[0043] It should be noted that if Figure 1 As shown, to achieve the contracted state of the underwater assembly, that is, to make the retractable isolation mechanism in a contracted and folded state, the sliding ring is first pulled up and the first spring is compressed, so that the upper connecting rod and the lower connecting rod are in a state close to being pulled into a line. At this time, the isolation cover is retracted to the periphery of the long cylinder, and then the sliding ring is released. The sliding ring is blocked by the ball plunger extending from the limit hole and cannot move downward, and the isolation cover remains in the retracted state. Figure 2 As shown, when the underwater component is to reach the relaxed and unfolded state, the blocking of the locking rod (the ball plunger at both ends) to the sliding ring must be released first. Specifically, the unlocking can be achieved by pushing the reset slider upward through the first trigger member to retract the ball plunger (it can also be achieved through the second trigger member). After unlocking, the sliding ring slides downward under the push of the first spring, and pushes the upper connecting rod and the lower connecting rod to move downward, wherein the lower connecting rod gradually changes from a nearly vertical state to a horizontal or nearly horizontal state, and the upper rod changes from a nearly vertical state to an inclined state (with an angle of about 30 degrees to the vertical direction). At this time, the connecting line between the two adjacent torsion springs is an edge of the lower end of the current isolation cover, which is in close contact with the underwater ground or sinks into the sediment. The expanded isolation cover is in the shape of a regular tetrahedron, and the area inside the isolation cover is the isolation zone, the top of the isolation zone is closed by the sliding ring and the wall of the long cylinder, and the gap between the sliding ring and the wall of the long cylinder is small, and the influence of the dormant eggs entering the isolation zone through the gap on the monitoring results can be basically ignored.

[0044] When the device provided by the present invention is deployed, the pull rope is slowly released to allow the long cylinder to sink vertically under the action of its own weight and the counterweight. When sinking to the bottom, the counterweight is first grounded, and the long cylinder continues to sink due to inertia and compresses the reset spring, which pushes the reset disk, reset rod and ball plunger to move upward. The ball plunger moves upward along the ball guide groove out of the limit hole, releasing the restriction on the sliding ring. Pushed by the main spring, the sliding ring opens the upper and lower connecting rods and unfolds a transparent film (isolation cover). The lower connecting rod is close to the sediment, and a closed sample square that is approximately a regular tetrahedron is formed in the isolation cover. In order to facilitate the determination of whether the isolation cover is opened underwater, a camera can also be set on the long cylinder for monitoring, so that researchers can directly observe the status of the isolation cover through the camera, including whether the isolation cover is opened underwater and whether it is damaged.

[0045] In one embodiment of the present invention, Figure 5 As shown, a cavity is provided in the locking rod 14 for loading the second spring 16, and through holes are opened at both ends of the locking rod 14 to communicate with the cavity. The tail of the ball plunger 15 is located in the cavity, and the head extends outward from the through hole. The tails of the two ball plungers 15 squeeze the second spring 16 from both ends, and the first trigger member is a reset spring 17. When the long cylinder 2 falls to the bottom of the water in a vertical direction and the counterweight block 6 contacts the bottom of the water, the self-weight of the long cylinder 2 will squeeze the reset spring 17 and push the reset slider 13 to move upward.

[0046] It should be noted that the reset slider is slidably connected to the bottom end of the long cylinder, which can keep the interior of the long cylinder basically watertight and can make a small range of axial movement under the push and pull of the reset rod.

[0047] In one embodiment of the present invention, a second triggering member is further included in the long cylinder 2, and the second triggering member includes an electric telescopic mechanism that can drive the locking rod 14 to move up and down. The long cylinder 2 is provided with a ball guide groove on the inner wall above the limiting hole for guiding the end of the ball plunger 15. There is no ball guide groove on the inner wall of the long cylinder below the limiting hole, which can prevent the ball plunger and the locking rod from moving downward beyond the limiting hole.

[0048] It should be noted that the electric telescopic mechanism included in the second trigger member can specifically be an electric telescopic rod (electric push rod) or a motor with a winding reel. One end of the telescopic rod or one end of the rope on the winding reel is connected to the locking rod to control the lifting and lowering of the locking rod.

[0049] In one embodiment of the present invention, a sampling valve and a sampling pump are provided on the sampling pipe, and the sampling valve and the sampling pump are located above the water surface.

[0050] It should be noted that the sampling pump is preferably arranged on the floating body and uses the battery on the floating body as the power source to pump water for sampling. It can be understood that the sampling pump can also be arranged in the long cylinder body.

[0051] In an embodiment of the present invention, there are four folding link rods, and the isolation cover 7 is in the shape of a regular square pyramid after being unfolded.

[0052] In an embodiment of the present invention, a signal lamp and a signal flag 18 are provided on the top of the floating body 1, and a battery for powering the signal lamp is provided inside or on the surface of the floating body 1.

[0053] In an embodiment of the present invention, a conduit 19 is provided in the long cylinder body 2. The lower end of the conduit 19 extends downward to the lower part of the long cylinder body 2 and communicates with the isolation area through a water intake hole 20 on the cylinder wall. The upper end of the conduit 19 passes through the upper end cover of the long cylinder body 2 and communicates with the lower end of the sampling tube. An electric valve is provided at the water intake hole 20.

[0054] It should be noted that the conduit can be arranged close to the inner wall of the long cylinder body or directly in the sandwich layer of the cylinder wall of the long cylinder body. The electric valve of the water intake hole is powered by the battery on the floating body or a waterproof and sealed battery stored in an appropriate place inside the long cylinder body.

[0055] It should be noted that by continuously sampling at intervals of a unit time and cooperating with the laboratory identification and counting of samples, the hatching dynamics of the dormant egg bank in the isolation area can be obtained to achieve the purpose of qualitative and quantitative analysis. For example, drawing the hatching time window (including the start of hatching, the peak of hatching, and the end of hatching) of different species, and continuously quantifying the hatching rate of dormant eggs of different species (XX pieces / day / square meter).

[0056] The present invention also provides a water pollution monitor, which includes a fully automatic microbial identification and analysis device and the above-mentioned in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies provides water samples collected in-situ for the fully automatic microbial identification and analysis device.

[0057] It should be noted that the fully automatic microbial identification and analysis device can be directly arranged on the floating body as needed, and automatically conducts automatic identification and analysis after automatic sampling, including the types of dormant eggs currently resuscitated and hatched, the density in the water sample, etc. The identification and analysis results can be uploaded to the cloud or a mobile terminal through a communication module, which is convenient for researchers to view in real time.

[0058] The above are only the preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. In-situ dormant egg bank germination qualitative and quantitative sampling device for water body, characterized in that, It includes a water surface component and a water bottom component. The water surface component includes a floating body (1) and a sampling tube. The water bottom component includes a long cylinder body (2), a fixing ring (3), a sliding ring (4), a first spring (5), a counterweight (6) and a retractable isolation mechanism. The long cylinder body (2) is arranged vertically. The fixing ring (3) is sleeved and fixed on the outer side wall of the top of the long cylinder body (2). The sliding ring (4) is slidably sleeved on the long cylinder body (2) and is located below the fixing ring (3). The first spring (5) is clamped between the fixing ring (3) and the sliding ring (4). The retractable isolation mechanism includes a flexible isolation cover (7) and a plurality of folding connecting rods that are evenly spaced and hinged at the circumference of the sliding ring (4) at the upper ends. The lower ends of the folding connecting rods are hinged to the counterweight (6). The folding connecting rods are used to contract and fold or expand and unfold the isolation cover (7). After the isolation cover (7) is contracted and folded, it closes and adheres to the outer wall of the long cylinder body (2). After the isolation cover (7) is expanded and unfolded, it forms an isolation area covering the surface of the underwater sediment. The floating body (1) is connected to the long cylinder body (2) by a pulling rope (8). The lower end of the sampling tube is underwater and communicates with the isolation area. The upper end of the sampling tube is above the water surface and is detachably connected to the floating body (1).

2. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies according to claim 1, characterized in that, Each of the folding connecting rods includes an upper connecting rod (9) and a lower connecting rod (10) that are end-to-end hinged. The upper end of the upper connecting rod (9) is hinged to the sliding ring (4). The lower end of the lower connecting rod (10) is hinged to the counterweight (6). The side wall of the isolation cover (7) is fixedly connected to each of the upper connecting rods (9). The upper end of the isolation cover (7) is fixedly connected to the periphery of the sliding ring (4), and the lower end extends to the hinged position of the upper connecting rod (9) and the lower connecting rod (10). A torsion spring (11) is provided at the end-to-end hinged position of the lower end of the upper connecting rod (9) and the upper end of the lower connecting rod (10).

3. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies according to claim 1, characterized in that Limiting holes are radially symmetrically opened on the wall of the long cylinder body (2). A reset rod (12), a reset slider (13), a locking rod (14) and a first trigger are arranged in the long cylinder body (2). The upper end of the reset rod (12) is fixedly connected to the midpoint of the horizontally arranged locking rod (14), and the lower end is fixedly connected to the reset slider (13). The reset slider (13) is slidably matched with the inner wall of the long cylinder body (2). The reset slider (13) is fixedly connected to the top surface of the counterweight (6) through the first trigger. Retractable ball plungers (15) are provided at both ends of the locking rod (14). After the isolation cover (7) is contracted and folded, the two ball plungers (15) protrude from the corresponding limiting holes and block and limit the sliding ring (4) from below. When the first trigger is triggered, it can push the reset slider (13) to move upward and make the ball plungers (15) retract into the limiting holes to release the limit on the sliding ring (4).

4. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies according to claim 3, characterized in that, A cavity for accommodating the second spring (16) is provided in the locking rod (14). Through holes communicating with the cavity are formed at both ends of the locking rod (14). The tail of the ball plunger (15) is located in the cavity, and the head extends outwards from the through hole. The tails of the two ball plungers (15) squeeze the second spring (16) from both ends. The first trigger is a return spring (17). When the long cylinder (2) falls vertically towards the bottom of the water and the counterweight (6) contacts the bottom ground, the self-weight of the long cylinder (2) will squeeze the return spring (17) and push the return slider (13) upwards.

5. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies according to claim 3, characterized in that, It further includes a second trigger provided in the long cylinder (2). The second trigger includes an electric telescopic mechanism capable of driving the locking rod (14) to move up and down. A ball guide groove for guiding the end of the ball plunger (15) is provided on the inner wall of the long cylinder (2) at a position above the limit hole.

6. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies according to claim 1, characterized in that, A sampling valve and a sampling pump are provided on the sampling pipe, and the sampling valve and the sampling pump are located above the water surface.

7. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies according to claim 1, characterized in that There are four folding linkages, and the isolation cover (7) is in the shape of a regular square pyramid after being unfolded.

8. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies according to claim 1, characterized in that A signal lamp and a signal flag (18) are provided on the top of the floating body (1), and a battery for powering the signal lamp is provided in the floating body (1).

9. The in-situ dormant egg bank germination qualitative and quantitative sampling device for water bodies according to any one of claims 1 to 8, characterized in that, A conduit (19) is provided in the long cylinder (2). The lower end of the conduit (19) extends downwards to the lower part of the long cylinder (2) and communicates with the isolation area through a water intake hole (20) on the cylinder wall. The upper end of the conduit (19) passes through the upper end cover of the long cylinder (2) and communicates with the lower end of the sampling pipe. An electric valve is provided at the water intake hole (20).

10. A water pollution monitor, characterized in that, It includes a fully automatic microbial identification and analysis device and the in-situ dormant egg bank germination qualitative and quantitative sampling device according to any one of claims 1 to 9. The in-situ dormant egg bank germination qualitative and quantitative sampling device provides a water sample collected in-situ for the fully automatic microbial identification and analysis device.

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