Photosynthetic bacteria activity protective agent and protection method thereof

Abstract

The invention relates to the technical field of bacterial activity protective agents, in particular to a photosynthetic bacteria activity protective agent and a protective method thereof.The protective agent comprises at least two anaerobic clostridium bacteria and strains which have high tolerance to a high-salt environment and can digest protein and nucleic acid and generate organic acid, the anaerobic clostridium bacteria comprise E2RT, BA2-13T and FH052T, and the strains have high tolerance to the high-salt environment and can digest protein and nucleic acid and generate organic acid; wherein the rDNA sequence of E2RT16S is as shown in SEQIDNO.1, the rDNA sequence of BA2-13T16S is as shown in SEQIDNO.2, and the rDNA sequence of FH052T16S is as shown in SEQIDNO.3. The invention also discloses a method for preparing the E2RT16S recombinant DNA. According to the photosynthetic bacterium activity protective agent and the protection method thereof, the problem that the activity stability of a photosynthetic bacterium agent is poor is solved, and the shelf life is prolonged.

Description

Technical Field

[0001] The present invention relates to the technical field of bacterial activity protectants, and particularly relates to a photosynthetic bacteria activity protectant and a protection method therefor. Background Art

[0002] In modern agricultural production, photosynthetic bacteria agents have become one of the key biotechnological products for improving aquaculture efficiency and promoting the sustainable development of agricultural planting due to their unique biological characteristics and ecological functions. Photosynthetic Bacteria (PSB), as microorganisms capable of performing photosynthesis under light or anaerobic conditions, can not only effectively decompose organic pollutants and purify water quality, but also provide rich nutrition for aquatic organisms. At the same time, in agricultural planting, they can promote soil improvement and plant growth and development.

[0003] Currently, the photosynthetic bacteria agents widely used in the market mainly exist in liquid form, which is convenient for application and can quickly play a role. However, this form of existence also brings a significant challenge: the problem of activity stability. The bacteria in the photosynthetic bacteria liquid are in a non-dormant state, with active metabolism, and are extremely sensitive to environmental conditions, especially changes in temperature and storage time. High temperature, low temperature or long-term storage may cause a decrease in the viability of the bacteria or even death, resulting in the inactivation of the liquid, which greatly limits the effective shelf life of the photosynthetic bacteria agent, usually difficult to exceed 6 months, seriously affecting the market competitiveness and application effect of the product.

[0004] To solve the above problems, scientific research personnel have been committed to developing technologies and products that can effectively protect the activity of photosynthetic bacteria. Traditional methods such as low-temperature refrigeration and adding nutrients can extend the shelf life to a certain extent, but the effect is limited and the cost is high, making it difficult to meet the needs of large-scale production and long-term storage. Therefore, the research and development of a new type of photosynthetic bacteria activity protectant and its protection method have become the key to improving the stability of photosynthetic bacteria agents and extending their shelf life. Based on this, the present invention proposes a photosynthetic bacteria activity protectant and a protection method therefor. Summary of the Invention

[0005] The purpose of the present invention is to provide a photosynthetic bacteria activity protectant and a protection method therefor, to solve the problem of poor activity stability of photosynthetic bacteria agents and extend the shelf life.

[0006] In a first aspect, the present invention provides a photosynthetic bacteria activity protectant, including at least two anaerobic Clostridium bacteria species with high tolerance to high-salt environments and capable of digesting proteins and nucleic acids to produce organic acids, and the anaerobic Clostridium bacteria species include E2R T , BA2-13 T and FH052 T , where E2R TThe 16S rDNA sequence is as shown in SEQ ID NO.1, BA2-13 T The 16S rDNA sequence is as shown in SEQ ID NO.2, FH052 T The 16S rDNA sequence is as shown in SEQ ID NO.3

[0007] Furthermore, the anaerobic Clostridium bacterium E2R T is extracted from biogas liquid, and its screening method includes: using Columbia blood agar medium, incubating for 5 days under anaerobic conditions at a pH value of 7.0 and a temperature of 35°C to obtain colonies that are light beige, opaque, shiny, slightly raised, and circular, with an overall edge diameter of up to 1.4 mm

[0008] Furthermore, the Columbia blood agar medium is 0.4 g ammonium sulfate, 0.2 g dipotassium hydrogen phosphate, 0.2 g potassium nitrate, 1.0 g sodium carbonate, 0.1 g calcium chloride, 0.2 g magnesium chloride heptahydrate, 0.69 mg ferric sulfate, 0.5 g yeast extract, 0.5 g peptone, 0.3 g glucose, and 10 mL trace element solution per liter of medium. Among them, the trace element solution includes 2.8 g / L boric acid, 0.04 g / L copper nitrate trihydrate, 0.75 g / L sodium molybdate dihydrate, 2.1 g / L manganese sulfate tetrahydrate, and 0.24 g / L zinc sulfate heptahydrate

[0009] Furthermore, the anaerobic Clostridium bacterium BA2-13 T is extracted from the saline-alkali soil in Sichuan Province, and its screening method includes: adding 1 mg / L sodium tungstate monohydrate, 10 mM sodium acetate, and 10 mM sodium formate to DSMZ medium as a complex medium, culturing under anaerobic conditions at a pH value of 7.6 and a temperature of 37°C to obtain colonies that are light beige, opaque, shiny, slightly raised, and circular, with an overall edge diameter of up to 1.2 mm

[0010] Furthermore, the complex medium is 10.0 g tryptone, 5.0 g soy peptone, 5.0 g yeast extract powder, 5.0 g beef extract, 10.0 g glucose, 0.5 g L-cysteine hydrochloride, 0.001 g resazurin, 0.04 g potassium dihydrogen phosphate, 2.04 g dipotassium hydrogen phosphate, 0.4 g sodium bicarbonate, 5.08 g sodium chloride, 0.01 g calcium chloride, 0.22 g magnesium sulfate heptahydrate, 0.05 g manganese sulfate monohydrate, 1.0 g Tween 80, 1 mg sodium tungstate monohydrate, 0.82 g sodium acetate, 0.68 g sodium formate, and 5% methanol by volume per liter of medium

[0011] Furthermore, the anaerobic Clostridium bacterium FH052 TIt is extracted from biogas liquid for producing methane with cow dung as raw material, and its screening method includes: using PY medium, incubating for 5 days under anaerobic conditions with a pH value of 7.5 and a temperature of 30 °C, and obtaining colonies that are thin and semi-transparent with a smooth surface.

[0012] Furthermore, the PY medium is 10.0 g of tryptone, 5.0 g of yeast extract, 0.2 g of sodium carbonate, 1.0 g of potassium chloride, 0.3 g of L-cysteine hydrochloride dihydrate, and 0.25 g of glucose per liter of medium.

[0013] In a second aspect, the present invention provides a method for protecting a photosynthetic bacteria activity protector, and the steps include: adding the photosynthetic bacteria activity protector to the photosynthetic bacteria, and the added amount of the protector bacteria is such that its bacterial concentration accounts for 45% - 55% of the total bacterial concentration in the mixed liquid bacterial solution.

[0014] Furthermore, the photosynthetic bacteria is Rhodopseudomonas palustris, and its 16S rDNA sequence is as shown in SEQ ID NO.4. Its culture medium composition is: 1.0 g of ammonium chloride, 2 g of sodium acetate, 2 g of sodium glutamate, 0.1 g of magnesium chloride, 0.1 g of calcium chloride, 0.6 g of potassium dihydrogen phosphate, 0.4 g of dipotassium hydrogen phosphate, 1 g of yeast extract, 0.5 g of molasses, adding 1000 mL of distilled water, and adjusting the pH to 7.2.

[0015] Furthermore, the added amount of each Clostridium bacteria in the protector is 7% - 35% of the bacterial concentration of the total added amount of Clostridium bacteria.

[0016] The beneficial effects of the present invention are as follows: In the photosynthetic bacteria activity protector of the present invention, the halophilic Clostridium bacteria can use inorganic salts as nutrients while decomposing some organic substances such as proteins, and produce organic acids through metabolism. This biological characteristic can supplement the survival and metabolism of photosynthetic bacteria. It has strong tolerance to inorganic salts like photosynthetic bacteria and can use inorganic salts as nutrients, and can also digest the remains of dead photosynthetic bacteria, while producing organic acids to supply the physiological metabolism of photosynthetic bacteria. Thus, the halophilic Clostridium bacteria and photosynthetic bacteria can jointly form an internal cycle of the bacterial community, continuously maintaining the continuous physiological cycle of the survival, metabolism, and decomposition of photosynthetic bacteria, and extending the survival time of the photosynthetic bacteria community.

[0017] In addition, the halophilic Clostridium bacteria have strong environmental adaptability and can survive and maintain normal physiological metabolism in relatively high and low temperature environments. The halophilic Clostridium bacteria produce organic substances such as organic acids through metabolism to protect the photosynthetic bacteria cells, maintain the activity of photosynthetic bacteria, and enhance the adaptability of photosynthetic bacteria to high and low temperature environments. Description of the Drawings

[0018] To more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the accompanying drawings required for use in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can also be obtained based on these drawings.

[0019] Figure 1 Diagram of the photosynthetic bacteria liquid stored at room temperature for 6 months and the mixed bacteria liquid of Example 1; Figure 2 Diagram of the photosynthetic bacteria liquid stored at 40°C for 6 months and the mixed bacteria liquid of Example 1; Figure 3 Diagram of the photosynthetic bacteria liquid stored at -20°C for 6 months and the mixed bacteria liquid of Example 1; Figure 4 Diagram of the photosynthetic bacteria liquid stored at room temperature for 6 months and the mixed bacteria liquid of Example 2; Figure 5 Diagram of the photosynthetic bacteria liquid stored at 40°C for 6 months and the mixed bacteria liquid of Example 2; Figure 6 Diagram of the photosynthetic bacteria liquid stored at -20°C for 6 months and the mixed bacteria liquid of Example 2. Detailed implementation manners

[0020] The following clearly and completely describes the technical solutions of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0021] It should be noted that in the present invention, yeast extract, peptone, tryptone, soy peptone, yeast extract powder, and beef extract are all purchased from Sichuan Baoshun Biotechnology Co., Ltd., molasses is purchased from Jinan Jinjutian Biotechnology Co., Ltd., and the resazurin model is RT209503 - 5g.

[0022] The photosynthetic bacteria agent used in the present invention is Rhodopseudomonas palustris, and its 16S rDNA sequence is as shown in SEQ ID NO.4. The composition of its culture medium is: 1.0 g ammonium chloride, 2 g sodium acetate, 2 g sodium glutamate, 0.1 g magnesium chloride, 0.1 g calcium chloride, 0.6 g potassium dihydrogen phosphate, 0.4 g dipotassium hydrogen phosphate, 1 g yeast extract, 0.5 g molasses, add 1000 mL distilled water, and adjust the pH to 7.2.

[0023] The inoculation amount is 20%, and it is cultured under outdoor sunlight conditions for 15 days. The detected bacteria concentration is 1.96 billion / mL.

[0024] The measurement of the concentration of photosynthetic bacteria is carried out by the plate coating method. The composition of the plate medium is as follows: 0.1 g of ammonium chloride, 0.1 g of sodium bicarbonate, 0.02 g of dipotassium hydrogen phosphate, 0.5 acetic acid sodium, 0.02 g of magnesium sulfate heptahydrate, 0.05 g of sodium chloride, 1 mL of growth factor, 1 mL of trace element solution, 97 mL of distilled water, 2 g of agar powder, and the pH is 7.0.

[0025] Among them, the trace element solution includes 2.8 g / L of boric acid, 0.04 g / L of copper nitrate trihydrate, 0.75 g / L of sodium molybdate dihydrate, 2.1 g / L of manganese sulfate tetrahydrate, and 0.24 g / L of zinc sulfate heptahydrate.

[0026] After inoculation by plate coating, the surface of the medium is covered with sterilized liquid paraffin and placed under an incandescent lamp for cultivation. The cultivation temperature is 30 °C. The photosynthetic bacteria are red and smooth colonies, and the edge diameter is about 0.5 mm. The Clostridium bacterium strain 1 selected in the present invention is an anaerobic acetic acid-producing bacterium that digests protein ribose extracted from biogas liquid, and the gene detection result is E2R T ,E2R T The 16S rDNA sequence is as shown in SEQ ID NO.1.

[0027] The screening method is to use Columbia blood agar medium with the optimal pH value of 7.0. After incubating for 5 days at 35 °C under anaerobic conditions, the colonies of this strain are light beige, opaque, shiny, slightly convex and round, and the entire edge diameter can reach 1.4 mm.

[0028] The cultivation method is as follows: 0.4 g of ammonium sulfate, 0.2 g of dipotassium hydrogen phosphate, 0.2 g of potassium nitrate, 1.0 g of sodium carbonate, 0.1 g of calcium chloride, 0.2 g of magnesium chloride heptahydrate, 0.69 mg of ferric sulfate, 0.5 g of yeast extract, 0.5 g of peptone, 0.3 g of glucose, 10 mL of trace element solution per liter of medium. Use NaOH to adjust the pH value to 7. After sterilizing the medium, inject it into a constant temperature sealed culture tank and cultivate under anaerobic conditions at 35 °C until the cultivation concentration reaches 1.2 billion / mL.

[0029] The Clostridium bacterium strain 2 selected in the present invention is an anaerobic halophilic and alkaliphilic bacterium extracted from the saline-alkali soil in Sichuan Province, and the gene detection result is the registered strain BA2-13 T ,BA2-13 T The 16S rDNA sequence is as shown in SEQ ID NO.2.

[0030] Based on DSMZ medium, add 1 mg / L of sodium tungstate monohydrate, 10 mM of sodium acetate, and 10 mM of sodium formate, and adjust the pH value to 7.6. Incubate at 37°C. The colonies of this strain are light beige, opaque, shiny, slightly convex, and round, with a diameter of up to 1.2 mm at the entire edge.

[0031] The culture method is as follows: for every liter of medium, there are 10.0 g of tryptone, 5.0 g of soy peptone, 5.0 g of yeast extract powder, 5.0 g of beef extract, 10.0 g of glucose, 0.5 g of L-cysteine hydrochloride, 0.001 g of resazurin, 0.04 g of potassium dihydrogen phosphate, 2.04 g of dipotassium hydrogen phosphate, 0.4 g of sodium bicarbonate, 5.08 g of sodium chloride, 0.01 g of calcium chloride, 0.22 g of magnesium sulfate heptahydrate, 0.05 g of manganese sulfate monohydrate, 1.0 g of Tween 80, 1 mg of sodium tungstate monohydrate, 0.82 g of sodium acetate, 0.68 g of sodium formate, and methanol with a volume fraction of 5%. Adjust the pH value of the medium to 8.0 with sodium hydroxide. After sterilizing the medium, inject it into a constant-temperature sealed culture tank, and incubate under anaerobic conditions at 37°C after inoculation. Culture until the concentration reaches 800 million / mL.

[0032] The Clostridium bacterium strain 3 selected in the present invention is an anaerobic Clostridium bacterium extracted from biogas liquid for producing methane using cow dung as raw material. The gene detection result has a similarity of 99% with the registered strain FH052 T and the 16S rDNA sequence is as shown in SEQ ID NO.3. T 16S rDNA sequence is as shown in SEQ ID NO.3.

[0033] The screening method is PY medium, with the optimal pH value being 7.5. After incubating at 30°C for 5 days, the colonies of this strain are thin and semi-transparent with a smooth surface.

[0034] The culture method is as follows: for every liter of medium, there are 10.0 g of tryptone, 5.0 g of yeast extract, 0.2 g of sodium carbonate, 1.0 g of potassium chloride, 0.3 g of L-cysteine hydrochloride dihydrate, and 0.25 g of glucose. Adjust the pH value of the medium to 7.5 with sodium hydroxide. After sterilizing the medium, inject it into a constant-temperature sealed culture tank, and incubate under anaerobic conditions at 30°C after inoculation. Culture until the concentration reaches 1 billion / mL.

[0035] Example 1 Mix the photosynthetic bacteria liquid with Clostridium bacterium strain 1, Clostridium bacterium strain 2, and Clostridium bacterium strain 3 in a ratio of 1:0.2:0.4:0.4. The concentration of photosynthetic bacteria is approximately 980 million / mL, accounting for approximately 50.5% of the total bacterial concentration.

[0036] Comparative Example 1-1 The mixed bacterial solution and the photosynthetic bacteria stock solution were stored at room temperature for 6 months respectively, and the concentration of the photosynthetic bacteria in each sample was detected. The concentration of the photosynthetic bacteria in the mixed bacterial solution was about 920 million / mL, and the concentration was about 740 million / mL after the photosynthetic bacteria solution was diluted 2 times.

[0037] Comparative Example 1-2 The mixed bacterial solution and the photosynthetic bacteria stock solution were stored in a 40°C constant temperature incubator for 30 days, and each sample was observed. Flocculent precipitates appeared in the photosynthetic bacteria solution, the upper layer of the solution was clear and transparent, and most of the photosynthetic bacteria died. The mixed bacterial solution still showed a dark red color, and the detected concentration of the photosynthetic bacteria was about 890 million / mL.

[0038] Comparative Example 1-3 The mixed bacterial solution and the photosynthetic bacteria stock solution were stored in a -20°C freezer for 30 days, and each sample was observed after thawing. Flocculent precipitates appeared in the photosynthetic bacteria solution after thawing, the upper layer of the solution was clear and transparent, and most of the photosynthetic bacteria died. The mixed bacterial solution still showed a dark red color, and the detected concentration of the photosynthetic bacteria was about 910 million / mL.

[0039] Example 2 The photosynthetic bacteria solution was mixed with Clostridium sp. strain 1 and Clostridium sp. strain 2 at a ratio of 1:0.5:0.5. The concentration of the photosynthetic bacteria was about 980 million / mL, accounting for about 49.5% of the total bacterial concentration.

[0040] Comparative Example 2-1 The mixed bacterial solution and the photosynthetic bacteria stock solution were stored at room temperature for 6 months respectively, and the concentration of the photosynthetic bacteria in each sample was detected. The concentration of the photosynthetic bacteria in the mixed bacterial solution was about 900 million / mL, and the concentration was about 740 million / mL after the photosynthetic bacteria solution was diluted 2 times.

[0041] Comparative Example 2-2 The mixed bacterial solution and the photosynthetic bacteria stock solution were stored in a 40°C constant temperature incubator for 30 days, and each sample was observed. Flocculent precipitates appeared in the photosynthetic bacteria solution, the upper layer of the solution was clear and transparent, and most of the photosynthetic bacteria died. The mixed bacterial solution still showed a dark red color, and the detected concentration of the photosynthetic bacteria was about 860 million / mL.

[0042] Comparative Example 2-3 The mixed bacterial solution and the photosynthetic bacteria stock solution were stored in a -20°C freezer for 30 days, and each sample was observed after thawing. Flocculent precipitates appeared in the photosynthetic bacteria solution after thawing, the upper layer of the solution was clear and transparent, and most of the photosynthetic bacteria died. The mixed bacterial solution still showed a dark red color, and the detected concentration of the photosynthetic bacteria was about 840 million / mL.

[0043] Example 3 Mix the photosynthetic bacteria liquid with Clostridium bacterium strain 1 in a ratio of 2:3. The concentration of photosynthetic bacteria is approximately 784 million / mL, accounting for approximately 52.1% of the total bacterial concentration.

[0044] Comparative Example 3-1 Store the mixed liquid of photosynthetic bacteria liquid and Clostridium bacterium strain 1 in a constant temperature incubator at 40 °C for 30 days. The mixed liquid is clear and transparent, and the photosynthetic bacteria are dead.

[0045] Comparative Example 3-2 Store the mixed liquid of photosynthetic bacteria liquid and Clostridium bacterium strain 1 in the freezer of a -20 °C refrigerator for 30 days. After thawing, the concentration of photosynthetic bacteria is detected to be approximately 140 million / mL.

[0046] Example 4 Mix the photosynthetic bacteria liquid with Clostridium bacterium strain 2 in a ratio of 1:2. The concentration of photosynthetic bacteria is approximately 653 million / mL, accounting for approximately 55.1% of the total bacterial concentration.

[0047] Comparative Example 4-1 Store the mixed liquid of photosynthetic bacteria liquid and Clostridium bacterium strain 2 in a constant temperature incubator at 40 °C for 30 days. The color of the mixed liquid becomes lighter, flocculent precipitates appear, and most of the photosynthetic bacteria are dead.

[0048] Comparative Example 4-2 Store the mixed liquid of photosynthetic bacteria liquid and Clostridium bacterium strain 2 in the freezer of a -20 °C refrigerator for 30 days. Flocculent precipitates appear after the mixed liquid thaws, the upper layer of the solution is clear and transparent, and most of the photosynthetic bacteria are dead.

[0049] Finally, it should be noted that the above examples are only used to illustrate the present invention and do not limit the technical solutions described in the present invention; those of ordinary skill in the art should understand that the present invention can still be modified or equivalently replaced; and all technical solutions and their improvements that do not depart from the spirit and scope of the present invention should be covered by the scope of the claims of the present invention.

Claims

1. A photosynthetic bacteria activity protector, characterized in that, Comprising at least two anaerobic Clostridium bacteria species that have high tolerance to high-salt environments and are capable of digesting proteins and nucleic acids to produce organic acids, wherein the anaerobic Clostridium bacteria include E2R T , BA2-13 T and FH052 T , wherein the 16S rDNA sequence of E2R T is as shown in SEQ ID NO.1, the 16S rDNA sequence of BA2-13 T is as shown in SEQ ID NO.2, and the 16S rDNA sequence of FH052 T is as shown in SEQ ID NO.

3.

2. The photosynthetic bacteria activity protector according to claim 1, characterized in that, The anaerobic Clostridium bacterium E2R T It is extracted from biogas liquid, and its screening method includes: using Columbia blood agar medium, incubating for 5 days under anaerobic conditions with a pH value of 7.0 and a temperature of 35°C, and obtaining colonies that are light beige, opaque, shiny, slightly convex and round, with a total edge diameter of up to 1.4 mm.

3. The photosynthetic bacteria activity protecting agent according to claim 2, characterized in that, The Columbia blood agar medium is composed of 0.4 g of ammonium sulfate, 0.2 g of dipotassium hydrogen phosphate, 0.2 g of potassium nitrate, 1.0 g of sodium carbonate, 0.1 g of calcium chloride, 0.2 g of magnesium chloride heptahydrate, 0.69 mg of iron sulfate, 0.5 g of yeast extract, 0.5 g of peptone, 0.3 g of glucose and 10 mL of trace element solution per liter of the medium. Among them, the trace element solution includes 2.8 g / L of boric acid, 0.04 g / L of copper nitrate trihydrate, 0.75 g / L of sodium molybdate dihydrate, 2.1 g / L of manganese sulfate tetrahydrate and 0.24 g / L of zinc sulfate heptahydrate.

4. A photosynthetic bacteria activity protector according to claim 1, characterized in that, The anaerobic Clostridium bacterium BA2-13 T was isolated from the saline-alkali soil in Sichuan Province. The screening method includes: adding 1 mg / L of sodium tungstate monohydrate, 10 mM of sodium acetate, and 10 mM of sodium formate to the DSMZ medium as a complex medium, and culturing under anaerobic conditions at a pH value of 7.6 and a temperature of 37 °C to obtain colonies that are light beige, opaque, shiny, slightly convex, and circular, with an overall edge diameter of up to 1.2 mm.

5. A photosynthetic bacteria activity protectant according to claim 4, characterized in that, The complex medium is composed of 10.0 g of tryptone, 5.0 g of soy peptone, 5.0 g of yeast extract powder, 5.0 g of beef extract, 10.0 g of glucose, 0.5 g of L-cysteine hydrochloride, 0.001 g of resazurin, 0.04 g of potassium dihydrogen phosphate, 2.04 g of dipotassium hydrogen phosphate, 0.4 g of sodium bicarbonate, 5.08 g of sodium chloride, 0.01 g of calcium chloride, 0.22 g of magnesium sulfate heptahydrate, 0.05 g of manganese sulfate monohydrate, 1.0 g of Tween 80, 1 mg of sodium tungstate monohydrate, 0.82 g of sodium acetate, 0.68 g of sodium formate and 5% (v / v) of methanol per liter of the medium.

6. The photosynthetic bacteria activity protector according to claim 1, characterized in that The anaerobic Clostridium bacterium FH052 T was isolated from biogas liquid produced from cow dung for methane production. Its screening method includes: using PY medium, incubating for 5 days under anaerobic conditions at a pH value of 7.5 and a temperature of 30 °C to obtain colonies that are thin and semi-transparent with a smooth surface.

7. A photosynthetic bacteria activity protector according to claim 6, characterized in that, The PY medium is composed of 10.0 g of tryptone, 5.0 g of yeast extract, 0.2 g of sodium carbonate, 1.0 g of potassium chloride, 0.3 g of L-cysteine hydrochloride dihydrate and 0.25 g of glucose per liter of the medium.

8. A method for protecting the photosynthetic bacteria activity protector according to any one of claims 1-7, characterized in that the steps It includes: Adding the photosynthetic bacteria activity protector to the photosynthetic bacteria, and the added amount of the protector bacteria is such that its bacterial concentration accounts for 45% - 55% of the total bacterial concentration in the mixed liquid bacterial solution.

9. The protection method of the photosynthetic bacteria activity protectant according to claim 8, characterized in that, The photosynthetic bacteria is Rhodopseudomonas palustris, and its 16S rDNA sequence is as shown in SEQ ID NO.

4. Its medium composition is: 1.0 g of ammonium chloride, 2 g of sodium acetate, 2 g of sodium glutamate, 0.1 g of magnesium chloride, 0.1 g of calcium chloride, 0.6 g of potassium dihydrogen phosphate, 0.4 g of dipotassium hydrogen phosphate, 1 g of yeast extract, 0.5 g of molasses, adding 1000 mL of distilled water, and adjusting the pH to 7.

2.

10. The protection method of the photosynthetic bacteria activity protector according to claim 8, characterized in that, The added amount of each Clostridium bacterium in the protector is 7% - 35% of the bacterial concentration of the total added amount of Clostridium bacteria.

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