Fermentation functional bacterial agent and application thereof in treatment of feces and biogas residues

Abstract

The invention relates to the technical field of feces and biogas residue treatment, in particular to a fermentation functional bacterial agent and application thereof in feces and biogas residue treatment. The fermentation functional microbial agent comprises strains including acetic acid bacteria, cellulomicrobe, nitrogen-fixing bacteria and bifidobacterium longum; the fermentation functional bacterial agent provided by the invention obviously improves the treatment efficiency of manure and biogas residues; compared with the prior art, the method has the advantages that the aerobic fermentation time is shortened to 15-30 days, and the biogas residue decomposition process is accelerated; meanwhile, the content of each strain in the microbial inoculum is accurately controlled, so that the synergistic effect of each strain is more efficient, the activity of each strain in the manure biogas residues is improved, the organic matter decomposition capacity of each strain is enhanced, and the resource utilization rate is increased. The device can be widely applied to the technical field of excrement and biogas residue treatment.

Description

Technical Field

[0001] The present invention relates to the technical field of fecal sewage and biogas residue treatment, and specifically relates to a fermentation functional bacterium agent and its application in fecal sewage and biogas residue treatment. Background Technique

[0002] With the booming development of the livestock industry, the output of livestock and poultry fecal sewage is increasing day by day. According to reliable data statistics, the annual resource output of livestock and poultry manure in China is about 4 billion tons. Unfortunately, its comprehensive utilization rate is less than 75%. Although anaerobic fermentation technology has become mature in the field of fecal sewage treatment, and many large and medium-sized biogas projects have been built in China, the problem of disposal of a large amount of residues (biogas slurry and biogas residue) has become increasingly prominent.

[0003] As an economical and effective means of treating biogas residue, aerobic composting can not only reduce environmental pollution, but also supply fertilizer resources for agricultural production. However, in actual operation, the efficiency of biogas residue composting needs to be improved, and the time and cost required for treatment also need to be optimized.

[0004] Microorganisms play a key role in the material cycle and energy conversion in nature. Research shows that specific microbial flora can accelerate the decomposition and composting process of organic matter. Therefore, screening out a microbial functional bacterium agent that can effectively promote the composting of biogas residue has become the key breakthrough to solve the current biogas residue composting problem, and it is expected to significantly improve the composting efficiency, reduce the treatment cost, and achieve the dual goals of efficient resource utilization and environmental protection. Summary of the Invention

[0005] The purpose of the present invention is to provide a fermentation functional bacterium agent and its application in fecal sewage and biogas residue treatment to solve the problems raised in the above background technique.

[0006] To solve the above technical problems, the present invention provides the following technical solutions:

[0007] A fermentation functional bacterium agent, the bacteria strains it contains are Acetobacter sp., Cellulosimicrobium cellulans, Azotobacter sp. and Bifidobacterium longum; among them, the preservation number of Acetobacter is BNCC336433; the preservation number of Cellulosimicrobium cellulans is BNCC370709; the preservation number of Azotobacter is BNCC335805; the preservation number of Bifidobacterium longum is BNCC186483.

[0008] Preferably; the bacterial content of Acetobacter in the fermentation functional bacterium agent is 10 7 -10 9 cfu / mL, the bacterial content of Cellulosimicrobium cellulans is 10 7 -109 cfu / mL, the bacterial content of Azotobacter is 10 7 -10 9 cfu / mL, the bacterial content of Bifidobacterium longum is 10 5 -10 7 cfu / mL.

[0009] A preparation method of a fermentative functional bacterial agent, which comprises the following preparation steps:

[0010] (1) Activate Acetobacter in a yeast extract nutrient agar solid medium, then pick colonies and inoculate them into a nutrient agar liquid medium for cultivation to obtain an Acetobacter bacterial liquid;

[0011] (2) Activate Cellulomonas in a nutrient agar solid medium containing tryptone, then pick colonies and inoculate them into a nutrient agar liquid medium containing tryptone for activation and cultivation to obtain a Cellulomonas bacterial liquid;

[0012] (3) Activate Azotobacter in a nutrient agar solid medium containing yeast extract, then pick colonies and inoculate them into a nutrient agar liquid medium containing yeast extract for activation and cultivation to obtain an Azotobacter bacterial liquid;

[0013] (4) Activate Bifidobacterium longum in a peptone solid medium, then pick colonies and inoculate them into a peptone liquid medium for cultivation to obtain a seed liquid, and then inoculate the seed liquid into a new peptone liquid medium for cultivation to obtain a Bifidobacterium longum liquid;

[0014] (5) Mix the Acetobacter bacterial liquid, Cellulomonas bacterial liquid, Azotobacter bacterial liquid and Bifidobacterium longum liquid to obtain a microbial functional bacterial agent; the bacterial content of Acetobacter in the functional bacterial agent is 10 7 -10 9 cfu / mL, the bacterial content of Cellulomonas is 10 7 -10 9 cfu / mL, the bacterial content of Azotobacter is 10 7 -10 9 cfu / mL, the bacterial content of Bifidobacterium longum is 10 5 -10 7 cfu / mL.

[0015] Preferably; the culture temperature of Acetobacter is 30°C - 35°C, the culture temperature of Cellulomonas is 28°C - 30°C, the culture temperature of Azotobacter is 28°C - 30°C, and the culture temperature of Bifidobacterium longum is 35°C - 37°C.

[0016] Preferably; before mixing the bacterial liquids, the viable bacteria count of the Acetobacter, Cellulomonas and Azotobacter bacterial liquids is not less than 2.0×10 9cfu / mL, the number of viable bacteria in the Bifidobacterium longum bacterial liquid is not less than 2.0×10 7 cfu / mL.

[0017] Preferably; the main components and pH of the yeast extract nutrient agar solid medium are as follows: glucose 10.0g, yeast extract 10.0g, 20.0g of CaCO3, distilled water 1.0L, pH 7.0, sterilized at 121°C for 15 min;

[0018] The main components and pH of the nutrient agar solid medium containing tryptone are as follows: tryptone 15.0g, soy peptone 5.0g, 5.0g of NaCl, distilled water 1.0L, pH 7.0, sterilized at 121°C for 15 min;

[0019] The main components and pH of the nutrient agar solid medium containing yeast extract are as follows: yeast extract 1.0g, 0.5g of K2HPO4, 0.2g of MgSO4·7H2O, 0.1g of NaCl, mannitol 10.0g, congo red 0.025g, distilled water 1.0L, pH 7.0, sterilized at 121°C for 15 min;

[0020] The main components and pH of the peptone solid medium are as follows: peptone 15.0g, glucose 20.0g, yeast extract powder 2.0g, soluble starch 0.5g, sodium chloride 5.0g, L-cysteine 0.5g, tomato extract powder 5.0g, liver extract powder 2.0g, tween 80 1.0 mL, distilled water 1.0L, pH 7.0, sterilized at 121°C for 15 min.

[0021] A method for treating manure biogas residue with a fermentation functional bacterium agent, which comprises the following steps:

[0022] (1) Adjust the water content of the manure biogas residue to 55%-65%,

[0023] (2) Then inoculate the fermentation functional bacterium agent in claim 1 into the composting material at 0.5 mL / 100 g - 0.7 mL / 100 g, and perform aerobic fermentation for 15 days - 30 days.

[0024] Preferably; during composting, when the temperature of the compost pile is higher than 65°C, turning treatment is carried out.

[0025] Preferably; on the 4th day and the 10th day of composting, turning treatment is carried out to increase the oxygen supply inside the compost.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] (1) The fermentation functional bacterial agent of the present invention significantly improves the treatment efficiency of fecal sewage biogas residues. Compared with the prior art, the aerobic fermentation time is shortened, and the aerobic fermentation time is shortened to 15 - 30 days, accelerating the composting process of biogas residues.

[0028] (2) By adjusting the water content of fecal sewage biogas residues to 55% - 65%, it provides a suitable water environment for the growth and metabolism of microorganisms, further promoting the composting process. And controlling the water content can make the nutrient components in fecal sewage biogas residues more easily dissolved in water, facilitating the uptake and utilization by microorganisms, and accelerating the decomposition and transformation of organic matter.

[0029] (3) By adding a fermentation functional bacterial agent during the fermentation process, the composting degree of fecal sewage biogas residues can be effectively improved, the residue of uncomposted substances is reduced, and the biomass toxicity in the compost product is lowered.

[0030] (4) The content of each strain in the bacterial agent is precisely controlled, making the synergistic effect of each strain more efficient, enhancing the activity of each strain in fecal sewage biogas residues, strengthening its decomposition ability of organic matter, and improving the resource utilization rate.

[0031] (5) The reasonable setting of the inoculation amount of the strain (0.5 mL / 100 g - 0.7 mL / 100 g) avoids the overuse of the bacterial agent while ensuring the treatment effect, reducing the cost.

[0032] (6) The temperature control measures during composting help to maintain the appropriate temperature during the composting process, further promoting the activities of microorganisms and the decomposition of organic matter, and improving the compost quality.

[0033] Description of the drawings

[0034] Figure 1 It shows the change of composting temperature under the treatment of the fermentation functional bacterial agent in Example 2 of the present invention; among them, CK is the blank control group, and M is the microbial bacterial agent treatment group;

[0035] Figure 2 It shows the change of the germination index of Chinese cabbage seeds in the compost under the treatment of the microbial bacterial agent in Example 2 of the present invention; among them, CK is the blank control group, and M is the microbial bacterial agent treatment group;

[0036] Figure 3 It shows the change of the total nitrogen, ammonium nitrogen and nitrate nitrogen contents in the compost under the treatment of the microbial bacterial agent in Example 2 of the present invention; among them, CK is the blank control group, and M is the microbial bacterial agent treatment group;

[0037] Figure 4 It shows the change of the total organic carbon (TOC) in the compost under the treatment of the microbial bacterial agent in Example 2 of the present invention; among them, CK is the blank control group, and M is the microbial bacterial agent treatment group.

[0038] Figure 5 This is the effect of adding fermentation functional bacterial agents and comparative bacterial agents on the total nitrogen, total phosphorus, and total potassium contents of compost in Example 3 of the present invention.

[0039] Figure 6 This is the effect of adding fermentation functional bacterial agents and comparative bacterial agents on the ammonium nitrogen and nitrate nitrogen contents of compost in Example 3 of the present invention. Specific Embodiments

[0040] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Apparently, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0041] Example 1

[0042] The following are the specific preparation steps of the composite microbial bacterial agent and related comparative bacterial agents:

[0043] 1. Material preparation: In the present invention, Acetobacter (BNCC336433), Cellulomonas (BNCC370709), Azotobacter (BNCC335805), and Bifidobacterium longum (BNCC186483) were purchased from Hebei Beina Biotechnology Co., Ltd. Reagents such as beef extract, peptone, agar, and NaCl were purchased from Beijing Solarbio Science & Technology Co., Ltd. The biogas residue was taken from the experimental station of China Agricultural University.

[0044] 2. Bacterial strain activation:

[0045] a. Activation of Acetobacter: The freeze-dried powder of Acetobacter (BNCC336433) was fully dissolved with 0.5 mL of nutrient agar liquid medium (glucose 10.0 g, yeast extract 10.0 g, CaCO3: 20.0 g, distilled water 1.0 L, pH 7.0, sterilized at 121 °C for 15 min), and mixed evenly. Then, 0.5 mL of the bacterial suspension was pipetted onto a solid medium (adding 15.0 g of agar to the components of the liquid medium) plate and spread evenly with a spreader. The plate was placed in a constant temperature incubator and cultured at 30 °C for 96 h for activation.

[0046] b. Activation of Cellulomonas: The freeze-dried powder of Cellulomonas (BNCC370709) was fully dissolved with 0.5 mL of nutrient liquid medium (tryptone 15.0 g, soy peptone 5.0 g, NaCl 5.0 g, distilled water 1.0 L, pH 7.0, sterilized at 121 °C for 15 min), and mixed evenly. Then, 0.5 mL of the bacterial suspension was pipetted onto a solid medium (adding 15.0 g of agar to the components of the liquid medium) plate and spread evenly with a spreader. The plate was placed in a constant temperature incubator and cultured at 30 °C for 32 h for activation.

[0047] c. Activation of nitrogen-fixing bacteria: The freeze-dried powder of nitrogen-fixing bacteria (BNCC335805) was fully dissolved in 0.5 mL of nutrient agar liquid medium (1.0 g of yeast extract, 0.5 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.1 g of NaCl, 10.0 g of mannitol, 0.025 g of congo red, 1.0 L of distilled water, pH 7.0, sterilized at 121 °C for 15 min), and mixed evenly. Then, 0.5 mL of the bacterial suspension was pipetted onto the solid medium (adding 15.0 g of agar to the components of the liquid medium) plate and spread evenly with a spreader. The plate was placed in a constant temperature incubator and cultured at 30 °C for 48 h for activation.

[0048] d. Bifidobacterium longum: The freeze-dried powder of Bifidobacterium longum (BNCC186483) was fully dissolved in 0.5 mL of nutrient agar liquid medium (15.0 g of peptone, 20.0 g of glucose, 2.0 g of yeast extract powder, 0.5 g of soluble starch, 5.0 g of sodium chloride, 0.5 g of L-cysteine, 5.0 g of tomato extract powder, 2.0 g of liver extract powder, 1.0 mL of Tween 80, 1.0 L of distilled water, pH 7.0, sterilized at 121 °C for 15 min), and mixed evenly. Then, 0.5 mL of the bacterial suspension was pipetted onto the solid medium (adding 20.0 g of agar to the components of the liquid medium) plate and spread evenly with a spreader. The plate was placed in a constant temperature anaerobic incubator and cultured at 37 °C for 96 h for activation.

[0049] 3. Freezing storage and activation use of bacterial strains:

[0050] Pick 2 - 5 colonies from the plate and wash them into a 1.5 mL cryotube with an appropriate amount of 20% sterilized glycerol solution, seal it with a sealing film, and store it at -80 °C. Each time before use, take one tube of each strain, melt it at room temperature, and after melting, add the bacterial liquid to the corresponding liquid medium and mix evenly, then place it in a shaking incubator for enlarged culture at the corresponding temperature.

[0051] 4. Preparation of functional bacterial agent: The cultured Acetobacter (B), Cellulomonas (C), nitrogen-fixing bacteria (D), and Bifidobacterium longum (T) bacterial liquids were mixed evenly at a ratio of 1:1:1:1 to obtain a composite bacterial agent (M). The bacterial content of Bifidobacterium longum in the composite bacterial agent is 10 6 cfu / mL, and the bacterial contents of Acetobacter, Cellulomonas, and nitrogen-fixing bacteria are 10 8 cfu / mL.

[0052] Preparation of the comparative microbial agent: The cultured Acetobacter (B), Azotobacter (D), and Bifidobacterium longum (T) were respectively combined with the Cellulomonas (C) bacterial liquid to obtain bacterial liquids BC, CD, and CT; in addition, Acetobacter, Cellulomonas, Azotobacter, and Bifidobacterium longum were mixed at a volume ratio of 1:1:1:1 to obtain a composite microbial agent (M); for the four bacterial liquids, the content of Bifidobacterium longum in the composite microbial agent was 10 6 cfu / mL, and the bacterial contents of Acetobacter, Cellulomonas, and Azotobacter were 10 8 cfu / mL.

[0053] Example 2

[0054] The following are the experimental steps and data of the manure biogas residue added with the fermentation functional microbial agent and the blank control group:

[0055] 1. The experimental steps are as follows:

[0056] Adjust the initial moisture content of the manure biogas residue to be controlled between 55% - 65%. In this example, for controlling variables, the initial moisture content of the two groups of composts was controlled at 60%. Add the fermentation functional microbial agent according to the mass of the compost material. Add 0.5 mL of the fermentation functional microbial agent per 100 g of manure biogas residue; add the above functional microbial agent to the material and mix well. The experimental group added with the above functional microbial agent was named (M); at the same time, set the treatment without adding the microbial agent as the control group (CK), and conduct composting for 25 days.

[0057] Perform turning treatment at 4 days and 10 days of composting to increase the oxygen supply in the compost pile. If the compost temperature reaches above 65°C during the composting process, an additional turning treatment is added to cool down the compost pile.

[0058] 2. The sampling time and sampling point requirements are as follows:

[0059] Sampling is carried out at the upper, middle, lower, left, and right points of the compost pile on the 0th, 5th, 10th, 15th, 20th, and 25th days of composting, and the samples are mixed and stored at -20°C for the determination of physical and chemical indexes.

[0060] 3. The sampling results are detected as follows

[0061] (1) Changes in compost temperature

[0062] As Figure 1 shown, the highest temperatures of the control group CK and the treatment M with the microbial functional microbial agent were 62°C and 65.0°C respectively, and the number of days above 50°C was 5 days and 14 days respectively. It can be seen that adding the microbial functional microbial agent increased the temperature during the high-temperature period of composting, extended the time of the high-temperature period, and promoted the decomposition of the biogas residue.

[0063] (2) Changes in the compost seed germination index

[0064] AsFigure 2 As shown in the figure, at the end of composting, the germination indices of Chinese cabbage seeds in CK and M were 89.05% and 120.92% respectively. It can be seen that adding microbial functional inoculants can increase the germination index of compost and reduce its biological toxicity.

[0065] (3) Changes in nitrogen content of compost

[0066] As Figure 3 can be seen, at the end of composting, the contents of total nitrogen, ammonium nitrogen, and nitrate nitrogen in CK and M were 1.66%, 365.22 mg / kg, 477.31 mg / kg and 2.05%, 162.62 mg / kg, 842.86 mg / kg respectively. Adding functional inoculants can increase the contents of total nitrogen and nitrate nitrogen in the compost product, which are 23.49% and 76.58% higher than those of the control respectively. It can be seen that adding microbial inoculants can reduce nitrogen loss during composting, promote the conversion of ammonium nitrogen to nitrate nitrogen, and have a significant nitrogen-preserving effect.

[0067] (4) Changes in total organic carbon (TOC) of compost

[0068] As Figure 4 can be seen, during the whole composting process, adding functional inoculants can promote the conversion of organic carbon in biogas residue. The TOC content of group M at the end of composting was 19.50%. From the results of the carbon-nitrogen ratio, it can be seen that adding functional inoculants effectively reduced the carbon-nitrogen ratio of the materials and increased the maturity of biogas residue compost.

[0069] Example 3

[0070] The following are the comparison results between the biogas residue of manure added with fermentation functional inoculants and the biogas residue of manure added with comparative inoculants:

[0071] Preparation of comparative inoculants: The cultured Acetobacter (B), Azotobacter (D), and Bifidobacterium longum (T) were respectively combined with the cell suspension of Cellulomonas (C) to obtain cell suspensions BC, CD, and CT.

[0072] In addition, Acetobacter, Cellulomonas, Azotobacter, and Bifidobacterium longum were mixed in a volume ratio of 1:1:1:1 to obtain a compound inoculant (M). The content of Bifidobacterium longum in each of the above inoculants was 10 6 cfu / mL, and the cell contents of Acetobacter, Cellulomonas, and Azotobacter were 10 8 cfu / mL..

[0073] Composting method: Adjust the initial moisture content of the manure biogas residue regulating material to be between 55% and 65%. In this example, for controlling variables, the initial moisture content of the two groups of composting is controlled at 60%. For every 100 g of compost, inoculate 0.5 ml of bacterial liquid, mix evenly and place it in a reactor, and conduct aerobic fermentation for 25 days. Turn the pile on the 4th and 10th days of composting. When the compost temperature is higher than 65 °C, conduct additional pile-turning and cooling operations.

[0074] (1) Effects of inoculating different bacterial liquids on total nitrogen, total phosphorus, and total potassium in compost

[0075] As Figure 5 shown, after 25 days of composting, except for CK, the contents of total nitrogen, total phosphorus, and total potassium in the compost treated with the compound bacterial agent (M) are higher than those treated with other bacterial liquids. The order of nitrogen fixation ability from high to low is M > CT > CD > BC > CK. The nitrogen fixation effect of treatment M is the best, and the total nitrogen loss is reduced by 41.38% compared with CK. The total phosphorus and total potassium contents of treatment M reach 1.13% and 1.76% respectively, and increase by 117.31% and 40.80% respectively compared with CK.

[0076] (2) Effects of inoculating different bacterial liquids on ammonium nitrogen and nitrate nitrogen contents in compost

[0077] As Figure 6 shown, after 25 days of composting, the addition of bacterial agents can significantly increase the nitrate nitrogen content in the compost product, and the nitrate nitrogen content in the compost treated with the compound bacterial agent (M) is increased by 10.44% - 62.61% compared with the treatments with other bacterial liquids. NO3-N / NH4-N can reflect the degree of maturity of the compost product. It can be seen from the figure that the addition of bacterial agents can promote the maturity of the biogas residue, and the treatment effect of M is the best.

[0078] Experimental data analysis

[0079] Comparing all the above data, the following conclusions can be obtained:

[0080] 1. Example 2 shows that adding the compound microbial functional bacterial agent (M) can significantly increase the temperature during the high-temperature period of composting, extend the high-temperature period, and promote the maturity of the biogas residue compared with the blank control group (CK); improve the seed germination index of the compost, reduce biological toxicity; reduce nitrogen loss during the composting process, promote the conversion of ammonium nitrogen to nitrate nitrogen, and have a significant nitrogen preservation effect; promote the conversion of organic carbon in the biogas residue, reduce the carbon-nitrogen ratio of the material, and increase the maturity of the biogas residue compost.

[0081] 2. Example 3 shows that among different bacterial liquid treatments, the contents of total nitrogen, total phosphorus, and total potassium in the compost treated with the compound bacterial agent (M) are higher than those treated with other single or combined bacterial liquids. The nitrogen fixation effect is the best, the total nitrogen loss is reduced the most, and the contents of total phosphorus and total potassium increase significantly. In terms of the content of nitrate nitrogen, the treatment with the compound bacterial agent (M) is also superior to other bacterial liquid treatments, and it has the best effect on promoting the decomposition of biogas residue.

[0082] 3. Generally speaking, adding the compound microbial functional bacterial agent (M) has significant effects in promoting the decomposition of fecal sewage biogas residue compost, increasing nutrient content, reducing nitrogen loss, and promoting the transformation of organic substances, and is superior to the treatment effects of single bacterial agents or other combined bacterial agents.

[0083] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions in the specification are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A fermentation functional bacterium agent, characterized in that: The strains included are Acetobacter sp., Cellulosimicrobium cellulans, Azotobacter sp., and Bifidobacterium longum.

2. The fermentation functional bacterial agent according to claim 1, wherein: The bacterial content of Acetobacter in the fermentation functional bacterium agent is 10 7 -10 9 cfu / mL, the bacterial content of Cellulomonas is 10 7 -10 9 cfu / mL, the bacterial content of Azotobacter is 10 7 -10 9 cfu / mL, the bacterial content of Bifidobacterium longum is 10 5 -10 7 cfu / mL.

3. A preparation method of a fermentation functional bacterium agent, characterized in that: It includes the following preparation steps: (1) Activate Acetobacter sp. in yeast extract nutrient agar solid medium, then pick colonies and inoculate them into nutrient agar liquid medium for cultivation to obtain Acetobacter sp. bacterial liquid; (2) Activate Cellulosimicrobium cellulans in nutrient agar solid medium containing tryptone, then pick colonies and inoculate them into nutrient agar liquid medium containing tryptone for activation and cultivation to obtain Cellulosimicrobium cellulans bacterial liquid; (3) Activate Azotobacter sp. in nutrient agar solid medium containing yeast extract, then pick colonies and inoculate them into nutrient agar liquid medium containing yeast extract for activation and cultivation to obtain Azotobacter sp. bacterial liquid; (4) Activate Bifidobacterium longum in peptone solid medium, then pick colonies and inoculate them into peptone liquid medium for activation and cultivation to obtain Bifidobacterium longum liquid; (5) Mix the acetic acid bacteria solution, Cellulomonas bacteria solution, nitrogen-fixing bacteria solution, and Bifidobacterium longum bacteria solution to obtain a microbial functional bactericide; the bacterial content of acetic acid bacteria in the functional bactericide is 10 7 -10 9 cfu / mL, the bacterial content of Cellulomonas bacteria is 10 7 -10 9 cfu / mL, the bacterial content of nitrogen-fixing bacteria is 10 7 -10 9 cfu / mL, the bacterial content of Bifidobacterium longum is 10 5 -10 7 cfu / mL.

4. The preparation method of a fermentation functional bacterial agent according to claim 3, characterized in that: The cultivation temperature of Acetobacter sp. is 30°C - 35°C, the cultivation temperature of Cellulosimicrobium cellulans is 28°C - 30°C, the cultivation temperature of Azotobacter sp. is 28°C - 30°C, and the cultivation temperature of Bifidobacterium longum is 35°C - 37°C.

5. The preparation method of a fermentation functional bacterial agent according to claim 3, characterized in that: Before mixing the bacterial solutions, the viable count of the Acetobacter, Cellulomonas, and Azotobacter bacterial solutions is not less than 2.0×10 9 cfu / mL, and the viable count of the Bifidobacterium longum bacterial solution is not less than 2.0×10 7 cfu / mL.

6. The preparation method of a fermented functional bacterium agent according to claim 3, wherein: The main components and pH of the yeast extract nutrient agar solid medium are as follows: glucose 10.0 g, yeast extract 10.0 g, 20.0 g of CaCO3, distilled water 1.0 L, pH 7.0, sterilized at 121°C for 15 min; The main components and pH of the nutrient agar solid medium containing tryptone are as follows: tryptone 15.0 g, soy peptone 5.0 g, 5.0 g of NaCl, distilled water 1.0 L, pH 7.0, sterilized at 121°C for 15 min; The main components and pH of the nutrient agar solid medium containing yeast extract are as follows: yeast extract 1.0 g, 0.5 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.1 g of NaCl, mannitol 10.0 g, congo red 0.025 g, distilled water 1.0 L, pH 7.0, sterilized at 121°C for 15 min; The main components and pH of the peptone solid medium are as follows: peptone 15.0 g, glucose 20.0 g, yeast extract powder 2.0 g, soluble starch 0.5 g, sodium chloride 5.0 g, L-cysteine 0.5 g, tomato extract powder 5.0 g, liver extract powder 2.0 g, tween 80 1.0 mL, distilled water 1.0 L, pH 7.0, sterilized at 121°C for 15 min.

7. A method for treating manure biogas residues by using a fermentation functional bacterium agent, characterized in that: It includes the following steps: (1) Adjust the water content of the manure biogas residue to 55% - 65%; (2) Then inoculate the fermented functional bacterium agent in claim 1 into the compost material at 0.5 mL / 100 g - 0.7 mL / 100 g, and perform aerobic fermentation for 15 days - 30 days.

8. A method for treating manure biogas residue by using a fermentation functional bacterial agent according to claim 7, characterized in that: During composting, when the temperature of the compost pile is higher than 65°C, perform turning treatment.

9. According to the method for treating manure biogas residue by using a fermentation functional bacterium agent in claim 7, it is characterized in that: On the 4th and 10th days of composting, the compost was turned to increase the oxygen supply inside the compost.

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