LED light source for protecting retina cells of human eyes and application of LED light source

Abstract

The invention provides an LED light source for protecting retina cells of human eyes, the luminescent spectrum of the LED light source is a continuous spectrum of 380-800nm, the LED light source does not contain ultraviolet light which is less than 380nm and is harmful to the human eyes, and the luminous intensity of the spectrum at the wavelength of 750-800nm is maximum. Compared with the prior art, the LED light source provided by the invention can effectively protect human eye retinal pigment epithelial cells while providing good illumination efficiency, and the LED light source is economical, durable, energy-saving, environment-friendly and suitable for large-scale popularization.

Description

Technical Field

[0001] The present invention belongs to the technical field of optical lighting, and particularly relates to an LED light source for protecting human eye retinal cells and its application. Background Art

[0002] Modern lifestyle enables humans to spend a large amount of time living under artificial light sources every day. With the development of technology, LED light sources are currently the most dominant type of artificial lighting in the lighting market. It has advantages such as energy conservation, environmental protection, economy and durability. From indoor lighting fixtures to the panels of electronic devices, LEDs have become an indispensable part of life.

[0003] LED light sources have a spectral composition that is completely different from natural light. Currently, LED light sources for indoor lighting generally use blue LED chips plus yellow phosphors to form white light. Although this method is simple and has a low cost, the obtained white light LED light sources generally have a high color temperature, a low color rendering index, and a large blue light component, which causes great harm to the cells of the human eye retinal pigment epithelium layer. There are also some LED light sources that use blue LED chips plus phosphors with green, yellow, and red light emissions to form white light in order to achieve a higher color rendering index or a lower color temperature. Although these light sources can all emit white light distinguishable by the human eye, the actual spectral compositions are significantly different. Although the differences in these spectra cannot be distinguished by the human eye, different light source spectra have obvious different effects on the human eye retinal pigment epithelial cells.

[0004] In recent years, many literatures have pointed out that long-term exposure to LEDs can cause permanent damage to the retina. The retinal pigment epithelium is a basic component of the retina and plays a crucial role in visual function. Damage to the structure and function of the retinal pigment epithelium can lead to various retinal diseases. Considering the mainstream position of LEDs in today's lighting market, it is of great significance to obtain an LED light source that is beneficial to protecting the human eye retinal pigment epithelium layer cells. Summary of the Invention

[0005] In view of this, the technical problem to be solved by the present invention is to provide an LED light source that can effectively protect human eye retinal pigment epithelium layer cells and its application.

[0006] The present invention provides an LED light source spectrum beneficial to protecting human eye retinal pigment epithelium layer cells and provides an LED packaging method for obtaining this spectrum.

[0007] The present invention provides an LED light source for protecting human eye cells. The emission spectrum of the LED light source is a continuous spectrum of 380-800 nm, and does not contain ultraviolet light harmful to the human eye that is less than 380 nm.

[0008] Preferably, the emission intensity of the emission spectrum is the highest at 750 - 800 nm. Taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at wavelengths of 380 nm - 425 nm is 1 - 15:100.

[0009] Preferably, taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at wavelengths of 425 - 500 nm is 1 - 30.

[0010] Preferably, taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at wavelengths of 500 - 550 nm is 5 - 40.

[0011] Preferably, taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at wavelengths of 550 - 600 nm is 6 - 50.

[0012] Preferably, taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at wavelengths of 600 - 650 nm is 10 - 60.

[0013] Preferably, taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at wavelengths of 650 - 700 nm is 15 - 80.

[0014] Preferably, taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at wavelengths of 700 - 750 nm is 20 - 90.

[0015] Preferably, a blue LED chip with a wavelength of 440 - 460 nm is used in combination with a yellow - green phosphor, a red phosphor, and a deep - red near - infrared phosphor to obtain an LED light source with the desired spectral form.

[0016] Preferably, the deep - red near - infrared phosphor includes YAl3B4O 12 :Cr 3+ , (Y,Gd)3(Al,Ga)5O 12 :Cr 3+ , (Y,Gd)3(Al,Sc)5O 12 :Cr 3+ , (Y,Gd)3(Ga,Sc)5O 12 :Cr 3+ , K3AlF6:Cr 3+ , Y3(Al,Sc)5O 12 :Cr 3+ , YAl3B4O12 : Cr 3+ , ScBO3:Cr 3+ , GaInO3:Cr 3+ , Mg4Nb2O9:Cr 3+ and one or more of LiScSi2O6:Cr 3+ ;

[0017] The red phosphor includes SrS:Eu 2+ , CaS:Eu 2+ , K2TiF6:Mn 4+ , K2TiF6:Mn 4+ or K2(Si,Ti)F6:Mn 4+ , Sr2Si5N8:Eu 2+ and one or more of CaAlSiN3:Eu 2+ ;

[0018] The yellow-green phosphor includes SrAl2O4:Eu 2+ , β-SIALON:Eu 2+ , YAGG:Ce 3+ , YAG:Ce 3+ and one or more of LuGG:Ce 3+ ;

[0019] The present invention provides an LED light source for protecting human eye retinal cells. The emission spectrum of the LED light source is a continuous spectrum of 380 - 800 nm, without ultraviolet light harmful to the human eye less than 380 nm, and the emission intensity of this spectrum is the largest at a wavelength of 750 - 800 nm. Compared with the prior art, the LED light source provided by the present invention can effectively protect the human eye retinal pigment epithelial cells while providing good lighting efficiency, and this LED light source is economical, durable, energy-saving and environmentally friendly, and is suitable for large-scale promotion. Description of the Drawings

[0020] Figure 1 is a photo of the LED light source prepared in Example 1 of the present invention;

[0021] Figure 2 is a photo of the LED light source prepared in Example 2 of the present invention;

[0022] Figure 3 is a photo of the LED light source prepared in Example 3 of the present invention;

[0023] Figure 4 is the emission spectrum diagram of the LED light sources obtained in Examples 1 - 3 of the present invention;

[0024] Figure 5Photos of the LED light sources obtained in Embodiments 1 to 3 of the present invention after being lit;

[0025] Figure 6 Graph showing the cell viability (CCK8 assay) results of retinal pigment epithelial cells ARPE-19 under darkness and illumination by light sources with three different spectra in the experiments of the embodiments of the present invention;

[0026] Figure 7 Graph showing the cell proliferation (Ki67 immunofluorescence assay) results of retinal pigment epithelial cells ARPE-19 under darkness and illumination by light sources with three different spectra in the embodiments of the present invention. Detailed implementation manners

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0028] The present invention provides an LED light source for protecting human eye retinal cells. The emission spectrum of the LED light source is a continuous spectrum of 380 - 800 nm, and does not contain ultraviolet light harmful to the human eye with a wavelength less than 380 nm.

[0029] According to the present invention, the emission spectrum of the LED light source does not emit light at wavelengths less than 380 nm, and has the maximum emission intensity at wavelengths of 750 - 800 nm.

[0030] In the present invention, the integrated emission intensity area refers to the area under the emission spectrum curve, that is, the integrated emission intensity area of 750 - 800 nm is the area under the emission spectrum curve within this wavelength range.

[0031] According to the present invention, taking the integrated emission intensity area of 750 - 800 nm as 100, the integrated emission intensity area at wavelengths of 380 nm - 425 nm is preferably 1 - 15, more preferably 1 - 12, still more preferably 2 - 12, still more preferably 5 - 12, and most preferably 6 - 10. In some embodiments provided by the present invention, the integrated emission intensity area at wavelengths of 380 nm - 425 nm of the emission spectrum is specifically 7.70337, 9.23739, or 6.51765.

[0032] According to the present invention, with the integrated area of the emission intensity at 750 - 800 nm being 100, the integrated area of the emission intensity in the wavelength range of 425 - 500 nm of the emission spectrum is preferably 1 - 30, more preferably 1 - 25, still more preferably 2 - 25, still more preferably 5 - 25, still more preferably 8 - 25, still more preferably 10 - 25, and most preferably 14 - 25. In some embodiments provided by the present invention, the integrated area of the emission intensity in the wavelength range of 425 - 500 nm of the emission spectrum is specifically 22.87202, 24.55105 or 14.11765.

[0033] According to the present invention, with the integrated area of the emission intensity at 750 - 800 nm being 100, the integrated area of the emission intensity in the wavelength range of 500 - 550 nm of the emission spectrum is preferably 5 - 40, more preferably 5 - 35, still more preferably 8 - 30, still more preferably 5 - 25, still more preferably 5 - 20, still more preferably 8 - 20, still more preferably 10 - 20, still more preferably 12 - 18, and most preferably 14 - 17. In some embodiments provided by the present invention, the integrated area of the emission intensity in the wavelength range of 500 - 550 nm of the emission spectrum is specifically 16.64187, 16.82657 or 14.5369.

[0034] According to the present invention, with the integrated area of the emission intensity at 750 - 800 nm being 100, the integrated area of the emission intensity in the wavelength range of 550 - 600 nm of the emission spectrum is preferably 6 - 50, more preferably 10 - 45, still more preferably 10 - 40, still more preferably 10 - 35, and most preferably 13 - 32. In some embodiments provided by the present invention, the integrated area of the emission intensity in the wavelength range of 550 - 600 nm of the emission spectrum is specifically 21.79315, 20.98401 or 23.72193.

[0035] According to the present invention, with the integrated area of the emission intensity at 750 - 800 nm being 100, the integrated area of the emission intensity in the wavelength range of 600 - 650 nm of the emission spectrum is preferably 10 - 60, more preferably 10 - 55, still more preferably 10 - 50, still more preferably 10 - 45, still more preferably 15 - 40, still more preferably 18 - 38, still more preferably 20 - 38, still more preferably 24 - 38, still more preferably 26 - 38, and most preferably 28 - 37. In some embodiments provided by the present invention, the integrated area of the emission intensity in the wavelength range of 600 - 650 nm of the emission spectrum is specifically 29.93552, 28.31488 or 36.57754.

[0036] According to the present invention, taking the integrated area of the luminous intensity at 750 - 800 nm as 100, the integrated area of the luminous intensity at wavelengths of 650 - 700 nm in the emission spectrum is preferably 15 - 80, more preferably 20 - 75, still more preferably 20 - 70, still more preferably 20 - 65, still more preferably 20 - 60, still more preferably 25 - 60, still more preferably 28 - 60, still more preferably 30 - 60, still more preferably 35 - 55, still more preferably 38 - 52, still more preferably 40 - 50, and most preferably 42 - 48. The integrated area of the luminous intensity at wavelengths of 650 - 700 nm in the emission spectrum is specifically 44.89087, 42.25584, or 47.37968.

[0037] According to the present invention, taking the integrated area of the luminous intensity at 750 - 800 nm as 100, the integrated area of the luminous intensity at wavelengths of 700 - 750 nm in the emission spectrum is preferably 20 - 90, more preferably 30 - 90, still more preferably 35 - 90, still more preferably 40 - 90, still more preferably 45 - 85, still more preferably 48 - 80, still more preferably 50 - 80, still more preferably 55 - 76, still more preferably 58 - 74, still more preferably 60 - 74, and most preferably 63 - 72. The integrated area of the luminous intensity at wavelengths of 700 - 750 nm in the emission spectrum is specifically 63.44246, 63.64084, or 71.37968.

[0038] The LED light source provided by the present invention can be prepared by different combinations of phosphors. Specifically, in the present invention, a blue LED chip with a wavelength of 440 - 460 nm can be used in combination with a yellow - green phosphor, a red phosphor, and a deep - red near - infrared phosphor to obtain an LED light source with the desired spectral form.

[0039] According to the present invention, the yellow - green phosphor can be any yellow - green phosphor well - known to those skilled in the art without special limitations. Preferably, in the present invention, it includes but is not limited to SrAl2O4:Eu 2+ , β - SIALON:Eu 2+ , YAGG:Ce 3+ , YAG:Ce 3+ and LuGG:Ce 3+ or one or more of them.

[0040] According to the present invention, the red phosphor can be any red phosphor well - known to those skilled in the art without special limitations. Preferably, in the present invention, it includes but is not limited to SrS:Eu 2+ , CaS:Eu 2+ , K2TiF6:Mn 4+ , K2TiF6:Mn 4+ or K2(Si,Ti)F6:Mn 4+, Sr2Si5N8:Eu 2+ and CaAlSiN3:Eu 2+ or one or more of them.

[0041] According to the present invention, the deep red-near infrared phosphor may be a deep red-near infrared phosphor well-known to those skilled in the art without any special limitation. Preferably, the phosphor includes, but is not limited to, YAl3B4O 12 :Cr 3+ , (Y,Gd)3(Al,Ga)5O 12 :Cr 3+ , (Y,Gd)3(Al,Sc)5O 12 :Cr 3+ , (Y,Gd)3(Ga,Sc)5O 12 :Cr 3+ , K3AlF6:Cr 3+ , Y3(Al,Sc)5O 12 :Cr 3+ , YAl3B4O 12 :Cr 3+ , ScBO3:Cr 3+ , GaInO3:Cr 3+ , Mg4Nb2O9:Cr 3+ and LiScSi2O6:Cr 3+ or one or more of them.

[0042] More specifically, the mass ratio of the yellow-green phosphor, the red phosphor and the deep red-near infrared phosphor is preferably (0.5 - 1.5):(0.005 - 0.02):(0.02 - 0.1), more preferably (0.6 - 1.2):(0.008 - 0.018):(0.03 - 0.08), still more preferably (0.7 - 1.1):(0.01 - 0.018):(0.04 - 0.06), and most preferably (0.76 - 1.0):(0.011 - 0.016):(0.045 - 0.06).

[0043] Even more specifically, the LED light source uses a blue LED chip with a wavelength of 440 - 460 nm in combination with the phosphors SrAl2O4:Eu 2+ , the phosphor SrS:Eu 2+ , the phosphor CaAlSiN3:Eu 2+ , the phosphor GaInO3:Cr 3+ and the phosphor ScBO3:Cr 3 + to obtain the required spectral form.

[0044] Specifically, the phosphor SrAl2O4:Eu2+ 、Phosphor SrS:Eu 2+ 、Phosphor CaAlSiN3:Eu 2+ 、Phosphor GaInO3:Cr 3+ and phosphor ScBO3:Cr 3+ The mass ratio is preferably (0.5 - 1.5):(0.005 - 0.015):(0.003 - 0.007):(0.01 - 0.05):(0.01 - 0.05), more preferably (0.6 - 1.2):(0.005 - 0.012):(0.004 - 0.006):(0.02 - 0.04):(0.02 - 0.04), still more preferably (0.7 - 1):(0.006 - 0.01):(0.005 - 0.006):(0.02 - 0.03):(0.02 - 0.035), and most preferably (0.76 - 1):(0.006 - 0.01):(0.005 - 0.006):(0.025 - 0.03):(0.02 - 0.03).

[0045] According to the present invention, the above phosphors can be commercially available or prepared by methods well known to those skilled in the art without special limitations. Taking GaInO3:Cr 3+ as an example, it is preferably prepared under the following synthesis conditions: Using Ga2O3, In2O3, and Cr2O3 as raw materials, with a stoichiometric ratio of 1:1:0.0005 - 0.01, after grinding evenly, reacting in a high-temperature resistance furnace at 1100°C - 1400°C under air or inert gas protection for 2 - 6 hours, naturally cooling to room temperature, and then grinding evenly again and obtaining through powder selection; In the present invention, specifically, the raw materials Ga2O3, In2O3, and Cr2O3 can be 1:1:0.001 - 0.01, further specifically 1:1:0.002 - 0.008, still further specifically 1:1:0.004 - 0.006, and even further specifically 1:1:0.005.

[0046] The LED chip selected for the LED light source provided by the present invention has the strongest emission wavelength in the blue light range of 440 - 470nm. The blue light emitted by the blue light chip excites the phosphor coated on it, and the transmitted blue light of the chip plus the light emitted by the phosphor can emit a specific spectral form. In the present invention, the coating thickness of the phosphor is preferably 0.5 - 5mm, more preferably 0.5 - 3mm, still more preferably 0.8 - 2mm, and most preferably 1 - 1.5mm.

[0047] More specifically, a phosphor is coated on an LED chip for encapsulation to obtain an LED light source. The encapsulation method may be a method well-known to those skilled in the art without special limitations. In the present invention, it is preferably encapsulated with an LED optical encapsulation adhesive; the LED encapsulation adhesive is preferably an epoxy LED encapsulation adhesive, a silicone LED encapsulation adhesive or a polyurethane LED encapsulation adhesive. Specifically, the epoxy encapsulation adhesive may be Kraft K-9301 epoxy LED encapsulation adhesive, Chuan Yu L-800 epoxy resin, Dow Corning OE-8001, Lei Ning Leini LN-103AB, etc.; the silicone encapsulation adhesive may be optical encapsulation adhesives such as Dow Corning OE-7666, Dow Corning OE-7841, Dow Corning OE-7843, Dow Corning OE-7810 and Dow Corning OE-7820; the polyurethane encapsulation adhesive may be DuPont DUROPTIX OE-6650, DUROPTIX OE-6630, polyurethane PU adhesive of Shenzhen Dazhou Material Technology Co., Ltd., etc.

[0048] The LED light source provided by the present invention can effectively protect the retinal pigment epithelial cells of the human eye while providing good lighting efficiency, and the LED light source is economical, durable, energy-saving and environmentally friendly, and is suitable for large-scale promotion.

[0049] The present invention also provides an application of the above LED light source in the preparation of a light source for protecting retinal pigment epithelial cells of the eye.

[0050] The present invention also provides a lighting fixture, including the above LED light source.

[0051] According to the present invention, the lighting fixture may be a lighting fixture well-known to those skilled in the art without special limitations. In the present invention, it preferably includes, but is not limited to, a table lamp, a direct-down flat lamp, a strip lamp or a spotlight.

[0052] In order to further illustrate the present invention, the following examples are used to describe in detail an LED light source for protecting retinal cells of the human eye and its application provided by the present invention.

[0053] The reagents used in the following examples are all commercially available. The DMEM high-glucose medium and serum-free medium used in the examples are both purchased from Gibco Life Technologies, USA; the SrAl2O4:Eu used in the examples 2+ is a commercially available green long-afterglow phosphor with the strongest emission peak position at 520 nm; SrS:Eu 2+ is a commercially available red long-afterglow phosphor with the strongest emission peak position at 600 nm; CaAlSiN3:Eu 2+It is a commercially available 1113 type nitride red powder with the strongest emission peak at 650 nm; GaInO3:Cr 3+ (self-made); ScBO3:Cr 3+ It is a commercially available near-infrared phosphor with the strongest emission peak at 800 nm.

[0054] GaInO3:Cr 3+ Synthesis conditions: Using Ga2O3, In2O3, and Cr2O3 as raw materials, according to the stoichiometric ratio of 1:1:0.005, after grinding evenly, react in a high-temperature resistance furnace at 1100 - 1400 °C under air or inert gas protection for 2 - 6 hours, naturally cool to room temperature, and then grind evenly again and obtain it through powder selection.

[0055] Using SrAl2O4:Eu 2+ ; SrS:Eu 2+ ; CaAlSiN3:Eu 2+ ; GaInO3:Cr 3+ ; ScBO3:Cr 3+ Five kinds of phosphors are coated on a blue LED chip with the strongest emission wavelength at 440 - 470 nm (the coating thickness is 1 - 1.5 mm), and then encapsulated with Leini LN-103AB epoxy encapsulation glue to obtain an LED lamp with the required spectrum type. Its spectrum, formula composition, and the encapsulated LED chip are as follows:

[0056] Example 1: SrAl2O4:Eu 2+ 1 g; SrS:Eu 2+ 0.006 g; CaAlSiN3:Eu 2+ 0.005 g; GaInO3:Cr 3+ 0.025 g; ScBO3:Cr 3+ 0.03 g;

[0057] Example 2: SrAl2O4:Eu 2+ 0.76 g; SrS:Eu 2+ 0.01 g; CaAlSiN3:Eu 2+ 0.005 g; GaInO3:Cr 3 + 0.03 g; ScBO3:Cr 3+ 0.02 g.

[0058] Example 3: SrAl2O4:Eu 2+ 1 g; SrS:Eu 2+ 0.006 g; CaAlSiN3:Eu 2+ 0.006 g; GaInO3:Cr 3+0.025 g; ScBO3:Cr 3+ 0.03 g.

[0059] Figure 1 Photo of the LED light source prepared in Example 1.

[0060] Figure 2 Photo of the LED light source prepared in Example 2.

[0061] Figure 3 Photo of the LED light source prepared in Example 3

[0062] Figure 4 Emission spectrum diagram of the LED light sources obtained in Examples 1 to 3.

[0063] Figure 5 Photo of the LED light sources obtained in Examples 1 to 3 after being lit.

[0064] Table 1 Integral area ratio of the emission intensity of Examples 1 - 3 in different wavelength bands (taking the integral area of the emission intensity in the 750 - 100 nm wavelength band as 100)

[0065]

[0066] The effect of the LED light sources obtained in Example 1 and Example 2 on protecting retinal pigment epithelial cells was obtained by comparing the CCK8 cell viability assay and immunofluorescence assay of the ki67 proliferation marker on the basis of cell experiments. The specific experimental procedures are as follows.

[0067] To confirm that the LED spectral composition of the present invention can protect the retinal pigment epithelium, the present invention used the human retinal pigment epithelial cell line ARPE - 19 for experiments. The ARPE - 19 cells were cultured for 72 h in the dark and under light sources with different spectral compositions, and the changes in the viability and proliferation ability of ARPE - 19 cells grown under different light sources were observed.

[0068] Cell experiment: Effect of irradiation with different spectral light sources on the viability and proliferation ability of ARPR - 19 cells.

[0069] Experimental cells:

[0070] The human retinal pigment epithelial cell line ARPE - 19 cells were purchased from American Type Culture Collection (ATCC, USA). The human retinal pigment epithelial cell line ARPE - 19 was cultured in a DMEM high - glucose medium containing 10% FBS and 1% penicillin - streptomycin in a 5% CO2, 37 °C constant - temperature cell culture incubator.

[0071] Experimental method:

[0072] 1) Grouping and experimental design:

[0073] The well - growing ARPE - 19 cells were sub - cultured and divided into 4 groups: normal control group (cultured in the dark), incandescent lamp IL group, LED1 group (Example 1), and LED2 group (ordinary commercially available white - light LED lamp). In the present invention, the light intensity from the light source to the cell plane was set to 500 Lux for each group. The above 4 groups of cells were cultured for 72 hours.

[0074] 2) Detection method:

[0075] CCK8 cell viability detection:

[0076] The ARPE - 19 cell suspension was counted, and 2×10 3 cells per well were inoculated into a 96 - well culture plate and cultured with high - glucose DMEM medium containing 10% FBS. After the cells adhered overnight, the medium was replaced with high - glucose DMEM medium containing 10% FBS, and the cells in each group were exposed to light in the dark or of different spectra for culture. After 72 hours of culture, the cell proliferation was detected using a CCK - 8 kit: the original medium was aspirated, 100 μL of new serum - free medium and 10 μL of CCK - 8 reagent were added to each well, the culture plate was incubated in a cell culture incubator for 1 hour, and then the absorbance value at 450 nm was measured with an enzyme - linked immunosorbent assay (ELISA) reader. Each group had 5 replicate wells, and the well with medium and CCK - 8 reagent but no cells was used as the blank well. The OD value of each group was zeroed with reference to the blank well.

[0077] Ki67 proliferation marker immunofluorescence staining:

[0078] The ARPE - 19 cell suspension was counted, and 2×10 3 cells per well were inoculated into a 96 - well culture plate and cultured with high - glucose DMEM medium containing 10% FBS. After the cells adhered overnight, the medium was replaced with high - glucose DMEM medium containing 10% FBS, and the cells in each group were exposed to light in the dark or of different spectra for culture. After 72 hours, the 96 - well plate was taken out. It was washed 3 times with PBS. 50 μL of 4% paraformaldehyde fixative was added to fix for 15 minutes, then washed 3 times with PBS, 5 minutes each time. 0.3% triton was added for perforation and left standing for 5 minutes. It was washed 3 times with PBS, 5 minutes each time. 2% BSA was added for blocking for 1 hour, and then the primary antibody Ki67 was diluted at 1:200 and incubated overnight at 4 °C in a wet box. The next day, the 96 - well plate was taken out and washed 3 times with PBS, 5 minutes each time. The corresponding fluorescent secondary antibody of the primary antibody species was added to the 96 - well plate and incubated in the dark for 1 hour, then washed 3 times with PBS, 5 minutes each time. DAPI was added and stained for 5 minutes, then washed 2 times with PBS. The 96 - well plate was placed under a fluorescence microscope for observation and photography.

[0079] Experimental results:

[0080] After ARPE-19 cells were irradiated with light sources of different spectra in the dark for 72 h, the cell viability in the dark group was the highest; there was no statistical significance in the viability between the LED1 group and the incandescent lamp group, and it was slightly lower than that in the dark group; the viability in the LED2 group was the lowest. As Figure 6 shown.

[0081] After ARPE-19 cells were irradiated with light sources of different spectra in the dark for 72 h, the cell proliferation ability in the dark group was the strongest; the cell proliferation abilities of the LED1 group and the incandescent lamp group were similar and slightly lower than that in the dark group; the proliferation ability in the LED2 group was the weakest. As Figure 7 shown.

[0082] The above results confirm that the protective effect of LED1 on retinal pigment epithelial cells is similar to that of incandescent lamps. However, LEDs have advantages such as energy conservation and environmental protection, are more economical and durable than incandescent lamps, and are more conducive to market promotion.

[0083] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. However, the present invention is not limited thereto. Those skilled in the art can understand that within the technical concept scope of the present invention, the technical solutions of the present invention can be modified, or some technical features can be combined in any other way. These modifications or combinations do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of each embodiment of the present invention, and should be regarded as the content disclosed by the present invention and all fall within the protection scope of the present invention.

Claims

1. An LED light source for protecting human eye retinal cells, characterized in that, The emission spectrum of the LED light source is a continuous spectrum from 380 to 800 nm, without ultraviolet light harmful to the human eye that is less than 380 nm.

2. The LED light source according to claim 1, characterized in that, The emission intensity of the emission spectrum is the highest at 750 - 800 nm. Taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at a wavelength of 380 nm - 425 nm is 1 - 15:

100.

3. The LED light source according to claim 1, wherein Taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at a wavelength of 425 - 500 nm is 1 - 30.

4. The LED light source according to claim 3, characterized in that, Taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at a wavelength of 500 - 550 nm is 5 - 40.

5. The LED light source according to claim 1, characterized in that, Taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at a wavelength of 550 - 600 nm is 6 - 50.

6. The LED light source according to claim 1, characterized in that Taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at a wavelength of 600 - 650 nm is 10 - 60.

7. The LED light source according to claim 1, characterized in that, Taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at a wavelength of 650 - 700 nm is 15 - 80.

8. The LED light source according to claim 1, characterized in that, Taking the integrated area of the emission intensity at 750 - 800 nm as 100, the integrated area of the emission intensity of the emission spectrum at a wavelength of 700 - 750 nm is 20 - 90.

9. The LED light source according to claim 1, characterized in that An LED light source with the required spectral form is obtained by using a blue LED chip of 440 - 460 nm and combining it with yellow - green phosphor, red phosphor, and deep - red near - infrared phosphor.

10. The LED light source according to claim 9, characterized in that, The deep red-near infrared phosphor includes YAl3B4O 12 :Cr 3+ , (Y,Gd)3(Al,Ga)5O 12 :Cr 3+ , (Y,Gd)3(Al,Sc)5O 12 :Cr 3+ , (Y,Gd)3(Ga,Sc)5O 12 :Cr 3 + , K3AlF6:Cr 3+ , Y3(Al,Sc)5O 12 :Cr 3+ , YAl3B4O 12 :Cr 3+ , ScBO3:Cr 3+ , GaInO3:Cr 3+ , Mg4Nb2O9:Cr 3+ and one or more of LiScSi2O6:Cr 3+ ; The red phosphor includes SrS:Eu 2+ , CaS:Eu 2+ , K2TiF6:Mn 4+ , K2TiF6:Mn 4+ or K2(Si,Ti)F6:Mn 4+ , Sr2Si5N8:Eu 2+ and CaAlSiN3:Eu 2+ one or more of them; The yellow-green phosphor includes SrAl2O4:Eu 2+ , β-SIALON:Eu 2+ , YAGG:Ce 3+ , YAG:Ce 3+ and LuGG:Ce 3+ and one or more of them.

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