Method for improving dispersion degree of sample application spot edge of liquid quality control product

Abstract

The invention discloses a method for improving the dispersion degree of a sample spot edge of a liquid quality control product, which comprises the following steps: pretreating an EP tube loaded with the liquid quality control product: soaking the EP tube in a surfactant solution, directly draining water, and completely drying in a drying oven; the surfactant solution is one of a Tween-20 solution with the volume concentration of 1%-12%, a Tween-80 solution with the volume concentration of 2%-15% and a Triton-100 solution with the volume concentration of 5%-20%. The pre-treated EP tube is used for loading the liquid quality control product, so that the'coffee ring 'phenomenon can be eliminated in the subsequent sample application process of the liquid quality control product, the quality control product is dispersed more uniformly, the diameter of a sample application spot is not obviously influenced under treatment of different concentrations, and the treated liquid quality control product has the advantages of uniform sample application spot diameter and high quality. The subsequent immunohistochemical staining performance of the liquid quality control product is not influenced.

Description

Technical Field

[0001] The present invention belongs to the field of biotechnology, and particularly relates to a method for improving the spotting dispersion of liquid quality control products. Background Art

[0002] After a liquid quality control product is dropped onto a glass slide and during the drying process, due to a series of effects such as surface tension and electrostatic adsorption, the liquid droplet spreads into a spherical cap shape on the surface of the glass slide. At the interface between the glass slide and the liquid droplet, the evaporation rate of water is faster than that at the interface between the liquid droplet and the air (the edge of the liquid droplet > the center). As a result, the water molecules in the middle of the liquid droplet will carry the quality control product fragment particles to the edge between the liquid droplet and the glass slide for replenishment. These cell fragments will continuously flow from the center of the liquid droplet to the edge along with the water flow, thus forming an edge aggregation effect - the "coffee ring" phenomenon. This makes the distribution of the quality control product uneven and affects subsequent density adjustment and staining result judgment.

[0003] Currently, the consumables for carrying liquid quality control products are conventional commercially available EP tubes, usually made of materials such as PP (polypropylene), PC (polycarbonate), and PE (polyethylene). These EP tubes all exhibit the "coffee ring" phenomenon of liquid quality control products during use, and there are significant differences in the "coffee ring" phenomenon among EP tubes of different brands and batches.

[0004] Therefore, it is very necessary to find a solution to solve the "coffee ring" phenomenon during the spotting process of liquid quality control products. Summary of the Invention

[0005] To solve the above problems, the present invention provides a method for improving the edge dispersion of liquid quality control product spotting spots.

[0006] To achieve the above object, the present invention adopts the following technical means: The first aspect of the present invention provides a method for improving the edge dispersion of liquid quality control product spotting spots, and the method is to pre-treat the EP tube loaded with the liquid quality control product: soak the EP tube in a surfactant solution, then directly drain the water, and place it in an oven to be completely dried.

[0007] In some embodiments of the present invention, the surfactant solution is a Tween-20 solution with a volume concentration of 1% - 12%.

[0008] In some embodiments of the present invention, the surfactant solution is a Tween-80 solution with a volume concentration of 2% - 15%; In some embodiments of the present invention, the surfactant solution is a Triton-100 solution with a volume concentration of 5% - 20%.

[0009] In some embodiments of the present invention, the soaking treatment is normal temperature soaking treatment or high temperature soaking treatment.

[0010] In some embodiments of the present invention, the duration of the normal temperature soaking treatment is 8 - 12 hours.

[0011] In some embodiments of the present invention, the high temperature soaking treatment is soaking at 95 - 100 °C for 8 - 10 minutes.

[0012] In some embodiments of the present invention, the drying temperature is 35 - 37 °C.

[0013] In some embodiments of the present invention, the EP tubes are sample tubes made of polypropylene, polycarbonate, or polyethylene.

[0014] The second aspect of the present invention provides the application of the EP tubes prepared by the method described in the first aspect in the preparation of products related to liquid cytology quality control.

[0015] The third aspect of the present invention provides a liquid quality control product kit, including EP tubes prepared by the method described in the first aspect.

[0016] The fourth aspect of the present invention provides a method for preparing a liquid cytology quality control product, characterized in that: (1) After soaking the EP tubes with a surfactant solution, directly drain the water and place them in an oven to be completely dried; (2) Load the liquid cell suspension into the EP tubes processed in step (1) for storage. Preferably, the EP tubes loaded with the liquid quality control product are stored refrigerated at 4 °C.

[0017] In some embodiments of the present invention, the surfactant solution is one of a Tween-20 solution with a volume concentration of 1% - 12%, a Tween-80 solution with a volume concentration of 2% - 15%, or a Triton-100 solution with a volume concentration of 5% - 20%.

[0018] In some embodiments of the present invention, the soaking treatment is normal temperature soaking treatment or high temperature soaking treatment.

[0019] In some embodiments of the present invention, the duration of the normal temperature soaking treatment is 8 - 12 hours.

[0020] In some embodiments of the present invention, the high temperature soaking treatment is soaking at 95 - 100 °C for 8 - 10 minutes.

[0021] In some embodiments of the present invention, the drying temperature is 35 - 37 °C.

[0022] In some embodiments of the present invention, the EP tubes are sample tubes made of polypropylene, polycarbonate, or polyethylene.

[0023] The present invention also provides the application of the liquid cytology quality control product prepared by the method described in the fourth aspect in the preparation of immunohistochemical staining detection products.

[0024] Advantages of the present invention Compared with the prior art, the present invention has the following advantages: The present invention provides a method for improving the edge dispersion of the spotting spot of the liquid quality control product. The EP tube used to load the liquid quality control product is pretreated by soaking it at room temperature or at a temperature of 95-100°C in one of the following surfactants: a Tween-20 solution with a volume concentration of 1%-12%, a Tween-80 solution with a volume concentration of 2%-15%, or a Triton-100 solution with a volume concentration of 5%-20%. After directly draining the water, it is placed in an oven and completely dried, and then used to load the liquid quality control product. This can eliminate the "coffee ring" phenomenon during the subsequent spotting process of the liquid quality control product, make the quality control product more evenly dispersed, and has no obvious effect on the diameter of the spotting spot under different concentration treatments. At the same time, when the EP tube is pretreated by this method and loaded with the liquid quality control product, not only the "coffee ring" phenomenon is eliminated, but also the difference problems caused by commercially available EP tubes of different brands and batches are solved. And the liquid quality control product after this treatment has no impact on the subsequent immunohistochemical staining performance of the liquid quality control product. Description of the drawings

[0025] Figure 1 Shows the direct observation diagrams of the spotting of 4 groups of liquid cytology quality control products in Example 1 of the present invention; Figure 2 Shows the immunohistochemical staining detection result diagrams of 4 groups of liquid cytology quality control products in Example 1 of the present invention; Figure 3 Shows the observation diagrams of the spotting of 4 groups of liquid cytology quality control products stained with hematoxylin in Example 2 of the present invention; Figure 4 Shows the immunohistochemical staining detection result diagrams of 4 groups of liquid cytology quality control products in Example 2 of the present invention; Figure 5 Shows the observation diagrams of the spotting of 5 groups of liquid cytology quality control products (1), (2), (5), (8), (10) stained with hematoxylin in Example 3 of the present invention; Figure 6 Shows the immunohistochemical staining detection result diagrams of 8 groups of liquid cytology quality control products (1)-(4), (6)-(9) in Example 3 of the present invention; Figure 7 Shows the observation diagrams of the spotting of 4 groups of liquid cytology quality control products stained with hematoxylin in Example 4 of the present invention; Figure 8Shows the direct observation diagrams of spotting of two groups of liquid quality control products in Example 5 of the present invention; Figure 9 Shows the immunohistochemical staining test result diagrams of two groups of liquid quality control products in Example 5 of the present invention; Figure 10 Shows the direct observation diagrams of spotting of two groups of liquid quality control products in Example 6 of the present invention; Figure 11 Shows the immunohistochemical staining test result diagrams of two groups of liquid quality control products in Example 6 of the present invention. Detailed implementation manners

[0026] The following examples are used herein to demonstrate the preferred implementation manners of the present invention. Those skilled in the art will understand that the technologies disclosed in the following examples represent the technologies discovered by the inventor that can be used to implement the present invention, and thus can be regarded as the preferred solutions for implementing the present invention. However, those skilled in the art should understand from this specification that many modifications can be made to the specific embodiments disclosed herein, and still obtain the same or similar results, without departing from the spirit or scope of the present invention.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention pertains. The materials cited herein and the materials they cite will be incorporated by reference. Those skilled in the art will recognize or be able to learn through routine experimentation many equivalent technologies to many of the specific embodiments of the invention described herein. These equivalents will be included in the claims.

[0028] The technical solutions of the present application will be further described in detail below in conjunction with the specific implementation manners.

[0029] Immunohistochemical detection method in subsequent examples: Use the LYNX480 fully automatic immunohistochemical staining machine of Tuling (Hangzhou) Biomedical Co., Ltd. for the experiment. The target primary antibody, secondary antibody, auxiliary reagents, etc. used are all from the commercial kits purchased from Tuling (Hangzhou) Biomedical Co., Ltd. The experiment adopts the conventional immunohistochemical experiment process and is carried out according to the instructions of the kit.

[0030] Example 1 (1) Load the liquid quality control product of Ramos cells using an EP tube made of conventional commercially available polypropylene material, and use the Plus60 fully automatic slide processing system of Tuling (Hangzhou) Biomedical Co., Ltd. for spotting. After drying, form a quality control spot as an untreated control.

[0031] (2) Immerse the EP tube made of conventional polypropylene material completely in ddH2O, treat it overnight at room temperature, drain the water, and then place it in an oven at 37°C until completely dried, and then load the liquid quality control product of Ramos cells.

[0032] (3) Soak the EP tubes made of polypropylene in 1% Tween-20 aqueous solution overnight, drain directly without washing, dry at 37°C, and then load the liquid quality control product of Ramos cells.

[0033] (4) Soak the EP tubes made of polypropylene in 10% Tween-20 aqueous solution overnight, drain directly without washing, dry at 37°C, and then load the liquid quality control product of Ramos cells.

[0034] Place all the EP tubes made of polypropylene loaded with the liquid quality control product of Ramos cells in steps (1), (2), (3), and (4) in cold storage at 4°C. Mix well before spotting and then perform the spotting operation. Observe directly, and the results are as Figure 1 shown.

[0035] The detection target corresponding to the liquid quality control product of Ramos cells is CD20. Use the above method to prepare the quality control slides by spotting the liquid quality control product, and use immunohistochemical staining to detect this target for performance verification. The results are as Figure 2 shown.

[0036] The results show that: as Figure 1 can be seen, for the liquid quality control product of Ramos cells loaded in the EP tubes without any treatment and the EP tubes soaked in ddH2O, after spotting, an edge aggregation effect appears and the distribution of the quality control product is uneven.

[0037] However, for the liquid quality control product of Ramos cells loaded in the EP tubes soaked in 1% Tween-20 and 10% Tween-20 aqueous solutions, after spotting, the distribution of the quality control product is uniform, and the degree of dispersion during the use of the liquid quality control product has nothing to do with the length of time the EP tubes are loaded with the liquid quality control product. The storage time is up to 6 months and the use effect is the same.

[0038] As Figure 2 can be seen, for the liquid quality control products of Ramos cells with different treatments in steps (1), (2), (3), and (4), after immunohistochemical staining detection, they all meet the staining requirements.

[0039] Example 2 (1) Use a conventional commercially available EP tube made of polyethylene to load the liquid quality control product of Ramos cells, and use the fully automatic slide processing system Plus60 of Tulin (Hangzhou) Biomedical Co., Ltd. for spotting. After drying, form a quality control spot as an untreated control.

[0040] (2) Place the EP tube made of conventional polyethylene in ddH2O and boil it for 10 minutes. Ensure that the EP tube is completely immersed in water under the boiling state. Drain the water, place it in an oven at 37 °C until completely dried, and then load the liquid quality control product of Ramos cells.

[0041] (3) Boil the EP tube made of conventional polyethylene in 1% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tube is completely immersed in water under the boiling state. Drain directly without washing. After drying at 37 °C, load the liquid quality control product of Ramos cells.

[0042] (4) Boil the EP tube made of conventional polyethylene in 10% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tube is completely immersed in water under the boiling state. Drain directly without washing. After drying at 37 °C, load the liquid quality control product of Ramos cells.

[0043] Place all the loaded quality control products in (1), (2), (3), and (4) in refrigerated storage at 4 °C. Mix well before spotting and then perform the spotting operation. Observe after hematoxylin staining. The results are as Figure 3 shown.

[0044] The detection target corresponding to the liquid quality control product of Ramos cells is CD20. The quality control slides are obtained by spotting the liquid quality control product prepared by the above method. The performance is verified by immunohistochemical staining to detect this target. The results are as Figure 4 shown.

[0045] The results show that: as Figure 3 can be seen, for the EP tubes without any treatment and the EP tubes treated by boiling in ddH2O, after loading the liquid quality control product of Ramos cells and spotting, an edge aggregation effect appears and the distribution of the quality control product is uneven.

[0046] However, for the EP tubes treated by boiling in 1% Tween-20 and 10% Tween-20 aqueous solutions and loaded with the liquid quality control product of Ramos cells, after spotting, the distribution of the quality control product is uniform.

[0047] As Figure 4 can be seen, for the liquid quality control products of Ramos cells treated in different ways in (1), (2), (3), and (4), after immunohistochemical staining detection, they all meet the staining requirements.

[0048] Example 3 (1) Use an EP tube made of conventional commercially available polycarbonate to load the liquid quality control product of Molt-4 cells, and use the fully automatic slide processing system Plus60 of Tulin (Hangzhou) Biomedical Co., Ltd. for spotting. After drying, form a quality control spot as an untreated control.

[0049] (2) For EP tubes made of conventional polycarbonate material, boil them in 1% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tubes are completely immersed in water under the boiling state, then directly drain them without cleaning. After drying at 37°C, load the liquid quality control product of Molt-4 cells.

[0050] (3) For EP tubes made of conventional polycarbonate material, boil them in 2% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tubes are completely immersed in water under the boiling state, then directly drain them without cleaning. After drying at 37°C, load the liquid quality control product of Molt-4 cells.

[0051] (4) For EP tubes made of conventional polycarbonate material, boil them in 4% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tubes are completely immersed in water under the boiling state, then directly drain them without cleaning. After drying at 37°C, load the liquid quality control product of Molt-4 cells.

[0052] (5) For EP tubes made of conventional polycarbonate material, boil them in 5% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tubes are completely immersed in water under the boiling state, then directly drain them without cleaning. After drying at 37°C, load the liquid quality control product of Molt-4 cells.

[0053] (6) For EP tubes made of conventional polycarbonate material, boil them in 6% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tubes are completely immersed in water under the boiling state, then directly drain them without cleaning. After drying at 37°C, load the liquid quality control product of Molt-4 cells.

[0054] (7) For EP tubes made of conventional polycarbonate material, boil them in 8% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tubes are completely immersed in water under the boiling state, then directly drain them without cleaning. After drying at 37°C, load the liquid quality control product of Molt-4 cells.

[0055] (8) For EP tubes made of conventional polycarbonate material, boil them in 10% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tubes are completely immersed in water under the boiling state, then directly drain them without cleaning. After drying at 37°C, load the liquid quality control product of Molt-4 cells.

[0056] (9) For EP tubes made of conventional polycarbonate material, boil them in 12% Tween-20 aqueous solution for 10 minutes. Ensure that the EP tubes are completely immersed in water under the boiling state, then directly drain them without cleaning. After drying at 37°C, load the liquid quality control product of Molt-4 cells.

[0057] (10)The EP tubes made of conventional polycarbonate material were boiled in 14% Tween-20 aqueous solution for 10 minutes. Under the boiling state of water, ensure that the EP tubes are completely immersed in water, then directly drain without cleaning, dry at 37°C, and load the liquid quality control product of Molt-4 cells.

[0058] All the loaded quality control products in (1)-(10) were stored refrigerated at 4°C.

[0059] A. Take the liquid quality control products corresponding to (1), (2), (5), (8), (10), mix them well before spotting, then perform the spotting operation, and observe after staining with hematoxylin. The results are as Figure 5 shown.

[0060] The results show that: as Figure 5 can be seen, for the liquid quality control product of Molt-4 cells loaded in the EP tubes without any treatment, after spotting, an edge aggregation effect appears and the distribution of the quality control product is uneven.

[0061] For the EP tubes treated by boiling in 1% Tween-20 - 10% Tween-20 aqueous solution for 10 minutes and loaded with the liquid quality control product of Molt-4 cells, after spotting, the distribution of the quality control product is uniform; Regarding the content of Tween-20, 1% is sufficient to significantly improve the "coffee ring" phenomenon of the quality control spot, and the improvement degree will not increase with the increase of the content of Tween-20. The improvement of the dispersion degree of the quality control spot is the same for the content of 1 - 12%, and there is no significant change in the diameter size of the spotting spot.

[0062] When it is greater than 12%, due to excessive residual Tween-20 (caused by not cleaning after boiling), the same phenomenon as directly adding Tween-20 to the solvent of the liquid quality control product will occur, and the quality control spot will spread excessively and exceed the qualified size range.

[0063] B. Take the liquid quality control products corresponding to (1)-(4), (6)-(9) to obtain quality control slides by spotting. The detection target of the liquid quality control product of Molt-4 cells is CD3. The quality control slides are used to detect this target by immunohistochemical staining for performance verification. The results are as Figure 6 shown.

[0064] The results show that: as Figure 6 can be seen, for the liquid quality control products of Molt-4 cells with different treatments in (1)-(4), (6)-(9), after immunohistochemical staining detection, they all meet the staining requirements, indicating that this operation has no influence on the subsequent immunohistochemical staining performance of the liquid quality control product.

[0065] Example 4 (1) Use a conventional commercially available EP tube to load the liquid quality control product of Molt-4 cells, without adding Tween-20. Use the fully automatic slide processing system Plus60 of Tuling (Hangzhou) Biopharmaceutical Co., Ltd. for spotting. After drying, a quality control spot is formed as an untreated control.

[0066] (2) Add 1% Tween-20 to the liquid quality control product of Molt-4 cells and load it using a conventional commercially available EP tube. (3) Add 5% Tween-20 to the liquid quality control product of Molt-4 cells and load it using a conventional commercially available EP tube. (4) Add 10% Tween-20 to the liquid quality control product of Molt-4 cells and load it using a conventional commercially available EP tube. Take the liquid quality control products corresponding to (1), (2), (3), and (4). After spotting and staining with hematoxylin, observe the results as Figure 7 shown.

[0067] The results show that adding the surfactant Tween-20, which is commonly used as a dispersant, to the liquid quality control product solvent can improve the spotting dispersion of the quality control product. At the same time, the improvement of the spotting dispersion of the quality control product increases with the increase in content. Adding 1% Tween-20 does not completely improve the "coffee ring" phenomenon of the quality control spot; and directly adding Tween-20 will continuously increase the diameter of the quality control spot and cause the quality control product to disperse continuously as the addition amount increases, exceeding the qualified range of the size of the quality control product spotting spot, posing a risk of contaminating tissue samples.

[0068] During the experiment, adding Tween-20 to the quality control product is more time-consuming and laborious compared to using a pre-treated EP tube to store the liquid quality control product and directly mixing and spotting when in use. And for pre-treating the EP tube, a concentration of 1% Tween-20 can achieve the desired dispersion effect.

[0069] Example 5 (1) Boil a conventional polypropylene EP tube in a 2% Tween-80 aqueous solution for 10 minutes. Under the boiling state of water, ensure that the EP tube is completely immersed in water, directly drain it without washing, dry it at 37°C, and then load the liquid quality control product of Ramos cells.

[0070] (2) Boil a conventional polypropylene EP tube in a 15% Tween-80 aqueous solution for 10 minutes. Under the boiling state of water, ensure that the EP tube is completely immersed in water, directly drain it without washing, dry it at 37°C, and then load the liquid quality control product of Ramos cells.

[0071] Place all the loaded quality control products in (1) and (2) in refrigerated storage at 4°C.

[0072] Take the liquid quality control products corresponding to (1) and (2). Mix well before spotting and then perform the spotting operation. Observe after hematoxylin staining. The results are as Figure 8 shown.

[0073] The results show that: as Figure 8 can be seen, for the liquid quality control products of EP tubes loaded with Ramos cells treated with 2% Tween-80 and 15% Tween-80 aqueous solutions boiled for 10 minutes, after spotting, the quality control products are evenly distributed, and the diameter of the spotting spots is within the appropriate range.

[0074] Take the liquid quality control products corresponding to (1) and (2) for spotting to obtain quality control slides. The detection target of the liquid quality control product of Ramos cells is CD20. The quality control slides are used for performance verification by immunohistochemical staining of this target. The results are as Figure 9 shown.

[0075] The results show that: as Figure 9 can be seen, for the liquid quality control products of Ramos cells treated differently in (1) and (2), after immunohistochemical staining detection, they all meet the staining requirements, indicating that this operation has no impact on the subsequent immunohistochemical staining performance of the liquid quality control products.

[0076] Example 6 (1) The EP tubes made of conventional polyethylene are completely immersed in 5% Triton-100 aqueous solution at room temperature for 10 hours, directly drained without washing, and dried at 37°C, then loaded with the liquid quality control product of Molt-4 cells.

[0077] (2) The EP tubes made of conventional polyethylene are completely immersed in 20% Triton-100 aqueous solution at room temperature for 10 hours, directly drained without washing, and dried at 37°C, then loaded with the liquid quality control product of Molt-4 cells.

[0078] All the loaded quality control products in (1) and (2) are stored in a refrigerator at 4°C.

[0079] Take the liquid quality control products corresponding to (1) and (2). Mix well before spotting and then perform the spotting operation. Observe after hematoxylin staining. The results are as Figure 10 shown.

[0080] The results show that: as Figure 10 can be seen, for the liquid quality control products of EP tubes loaded with Molt-4 cells treated with 5% Triton-100 and 20% Triton-100 aqueous solutions soaked at room temperature for 10 hours, after spotting, the quality control products are evenly distributed, and the diameter of the spotting spots is within the appropriate range.

[0081] The liquid quality control products corresponding to (1) and (2) are spotted to obtain a quality control glass slide. The detection target corresponding to the liquid quality control product of Molt-4 cells is CD3. The quality control glass slide is used for performance verification by immunohistochemical staining of this target. The results are as Figure 11 shown.

[0082] The results show that: as Figure 11 can be seen, for the liquid quality control products of Molt-4 cells with different treatments of (1) and (2), immunohistochemical staining detection is carried out, and all meet the staining requirements, indicating that this operation has no influence on the subsequent immunohistochemical staining performance of the liquid quality control products.

[0083] The overall experimental results show that adding a surfactant commonly used as a dispersant to the liquid quality control product solvent can improve the spotting dispersion of the quality control product, but its dispersion effect is affected by the addition amount. At the same time, directly adding the surfactant will also cause the diameter of the quality control spot to continuously expand with the increase of the addition amount, and the quality control product will continuously disperse, exceeding the qualified range of the size of the quality control product spotting spot, bringing the risk of contaminating the tissue sample.

[0084] Soaking commercially available EP tubes with a surfactant and then loading and storing the liquid quality control products can not only eliminate the "coffee ring" phenomenon of the spotting spot, but also solve the difference problems brought by commercially available EP tubes of different brands and batches; storing the liquid quality control products in the EP tubes treated by this method, its dispersion effect is not affected by the storage time and can be stably stored for 6 months; when using, it is directly mixed and spotted, which is more time-saving and labor-saving compared with adding a surfactant freshly prepared to increase the dispersion degree; moreover, storing the liquid quality control products in the EP tubes treated by this method has no influence on the subsequent immunohistochemical staining performance of the liquid quality control products.

[0085] All documents mentioned in this invention are cited in this application as references, just as if each document is cited separately as a reference. In addition, it should be understood that after reading the above teachings of this invention, those skilled in the art can make various changes or modifications to this invention, and these equivalent forms also fall within the scope defined by this application.

Claims

1. A method for improving the edge dispersion of the spotting spots of a liquid quality control product, characterized in that: The method is to pre-treat the EP tube loaded with liquid quality control product: after soaking the EP tube with a surfactant solution, directly drain the water and place it in an oven to be completely dried.

2. A method for improving the edge dispersion of the spotting spot of a liquid quality control product according to claim 1, characterized in that: The surfactant solution is one of a Tween-20 solution with a volume concentration of 1%-12%, a Tween-80 solution with a volume concentration of 2%-15%, and a Triton-100 solution with a volume concentration of 5%-20%.

3. A method for improving the edge dispersion of the spotting spots of a liquid quality control product according to claim 1, characterized in that: The soaking treatment is normal temperature soaking treatment or high temperature soaking treatment.

4. A method for improving the edge dispersion of the spotting spot of the liquid quality control product according to claim 3, characterized in that: The duration of the normal temperature soaking treatment is 8-12 hours.

5. A method for improving the edge dispersion of the spotting spots of a liquid quality control product according to claim 3, characterized in that: The high temperature soaking treatment is soaking at 95-100 °C for 8-10 minutes.

6. A method for improving the edge dispersion of the spotting spots of a liquid quality control product according to claim 1, characterized in that: The drying temperature is 35-37 °C.

7. A method for improving the edge dispersion of the spotting spots of a liquid quality control product according to any one of claims 1-6, characterized in that: The EP tube is a sample tube made of polypropylene, polycarbonate, or polyethylene.

8. Use of the EP tube prepared by the method according to any one of claims 1-6 or the EP tube according to claim 7 in the preparation of liquid cytology quality control related products.

9. A liquid quality control product kit, characterized in that: It includes the EP tube prepared by the method according to any one of claims 1-6 or the EP tube according to claim 7.

10. A method for preparing a liquid cytology quality control product, characterized in that: (1) The EP tube is pre-treated by the method according to any one of claims 1-6; (2) Load the liquid cell suspension into the pre-treated EP tube in step (1) for storage.

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