Method for preventing and treating wheat root rot

Through the method of combining astaxanthin-transgenic wheat or in vitro astaxanthin with agents, the prevention and treatment of wheat root rot has been solved, and efficient and environmentally friendly disease control has been achieved.

CN120266748APending Publication Date: 2025-07-08SHANDONG LINYI TOBACCO
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Patent Information

Application Number
CN202510265374.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-07
Publication Date
2025-07-08

AI Technical Summary

Technical Problem

The existing technology lacks effective disease-resistant varieties and natural green fungicides in the prevention and control of wheat root rot. Chemical control has problems of pesticide residues and drug resistance, and agricultural prevention and control measures are not enough to cope with the serious occurrence of diseases.

Method used

Astaxanthin transgenic technology is used to construct wheat plants or apply astaxanthin in vitro, combining a mixture of astaxanthin and wheat root rot prevention and control agents to prevent and treat wheat root rot.

Benefits of technology

It significantly reduces the incidence of wheat root rot by 60-70%, improves wheat resistance, is environmentally friendly, and simplifies the prevention and control process.

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Abstract

The invention provides a method for preventing and treating wheat root rot, which adopts a method of biological breeding, transgenosis, in-vitro application or mixed use with other medicaments to prevent and treat the wheat root rot, and can effectively prevent and treat the wheat root rot from the seedling emergence stage, the jointing stage to the heading stage. It is proved for the first time that a natural product astaxanthin can effectively prevent and treat wheat root rot, transgenic wheat of astaxanthin key synthetic genes shows obvious disease resistance after being inoculated with wheat root rot pathogenic bacteria, but wild type control wheat shows obvious susceptibility. The application of external astaxanthin is carried out on the basis of a transgenic plant, and the wheat also shows the characteristic of root rot resistance.
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Claims

1. A method for controlling wheat root rot, characterized in that, The method includes at least one of the following methods: Method 1: Constructing astaxanthin transgenic plants; Method 2: Ex vivo application of astaxanthin; Method 3: Ex vivo application of a mixed agent composed of astaxanthin and a wheat root rot control agent.

2. The control method of wheat root rot according to claim 1, characterized in that, The method for constructing astaxanthin transgenic plants includes: Connecting the key genes CBFD and HBFD for astaxanthin synthesis to an expression vector to obtain a recombinant expression vector; Transferring the recombinant expression vector to wheat immature embryo callus by particle bombardment; Post-treating the callus on an osmotic medium and then transferring it to an SD2 medium for dark culture, and then transferring it to a differentiation medium containing antibiotics for light culture to grow small seedlings; Transferring the small seedlings to a rooting medium containing antibiotics to induce rooting, and after rooting, transferring them to soil for continuous culture to obtain transformed plants; Conducting transgenic identification on the transformed plants to obtain wheat plants that can stably produce astaxanthin.

3. The control method of wheat root rot according to claim 1, characterized in that, The ex vivo application of astaxanthin includes: Mixing an astaxanthin aqueous emulsion with a concentration of 90 mg / kg - 110 mg / kg with wheat seeds, where 1 kg of wheat seeds uses 90 mg / kg - 110 mg / kg of astaxanthin; or spraying the astaxanthin aqueous emulsion with a concentration of 90 mg / kg - 110 mg / kg on wheat seedlings or adult plants.

4. The control method of wheat root rot according to claim 1, characterized in that, The wheat root rot control agent includes one of triadimefon, tebuconazole, epoxiconazole, and mancozeb wettable powder agents. The astaxanthin aqueous emulsion with a concentration of 90 mg / kg - 110 mg / kg is used in the ex vivo application of astaxanthin, and the mass ratio of the astaxanthin aqueous emulsion to the wheat root rot control agent is 1:1 - 4.

5. Application of astaxanthin or an organism containing astaxanthin in the control of wheat root rot.

6. The application according to claim 5, characterized in that The organism containing astaxanthin includes at least one of a nucleic acid molecule containing astaxanthin, a vector capable of expressing astaxanthin, and a transformant containing the vector.