Preparation method of long-acting insecticidal biological agent

Abstract

The invention discloses a long-acting insecticidal biological agent and a preparation method thereof. The preparation method comprises the following steps: step 1, preparing plant raw materials, treating and extracting to obtain a traditional Chinese medicine extract; step 2, mixing the traditional Chinese medicine extracts in the step 1, and adding beauveria bassiana, corn flour, biochar and an auxiliary agent at the same time; 3, uniformly mixing, adding water, and uniformly stirring to form a suspension at the temperature of 60-80 DEG C; 4, conveying the suspension liquid into dissociation equipment, and dispersing and dissociating the particles to generate small particles; and 5, grinding and screening through a drum-type screening machine. Compared with the prior art, the invention has the advantages of controlling the quantity of pests, reducing the harm of pests, improving the yield and quality of crops, having no influence on non-target organisms, prolonging the effective period, preventing pollution in the storage process, keeping stability and active ingredients, preventing decomposition under the condition of illumination or high temperature, and improving the quality of the crops. By measuring the activity of the beauveria bassiana in different metered quantities on different matrixes, different concentration gradients are set for the optimal concentration and the carrier respectively, and the optimal carrier is determined by measuring the germination rate of the beauveria bassiana.

Description

Technical Field

[0001] The present invention relates to the technical field of biological pesticides, and specifically to a preparation method of a long-acting insecticidal biological agent. Background Art

[0002] Biological pesticides are mainly divided into three categories: Bacillus thuringiensis, insect viruses, and plant extracts. They have the characteristics of convenient material collection, low cost, long control period, high efficiency, economy, safety, pollution-free, and high compatibility with the environment. They are the best pesticide choice for the current production of pollution-free green vegetables. Over time, various combinations of biological pesticides composed of biological bacteria have been developed to eliminate pests and reduce the damage caused by them. These biological pesticides use live fungi or bacteria as insect pathogens to kill insects. However, the insecticidal agents are susceptible to environmental conditions during storage, which affects the activity of their bacterial cells. Their stability is not high, it is difficult to effectively preserve them for a long time, and even inactivation and failure may occur.

[0003] The information disclosed in this background art section is only intended to increase the understanding of the overall background of the present invention, and should not be regarded as an admission or any form of suggestion that this information constitutes the prior art already known to those of ordinary skill in the art. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to overcome the above technical defects, and provide a preparation method of a long-acting insecticidal biological agent, which can eliminate underground pests and cause no harm, and at the same time has high stability and is not easily inactivated.

[0005] To solve the above problems, the technical solution of the present invention is a preparation method of a long-acting insecticidal biological agent, including the following steps;

[0006] Step 1: Prepare plant raw materials for treatment and extraction to obtain a traditional Chinese medicine extract;

[0007] Step 2: Mix and configure the traditional Chinese medicine extract in Step 1, and at the same time add Beauveria bassiana, corn starch, biochar, and additives;

[0008] Step 3: After uniformly mixing the mixture in Step 2, add a certain amount of water, stir evenly, and at a temperature of 60°C - 80°C, send the mixture into a drug suspension machine, and form a suspension through stirring and suspension;

[0009] Step 4: Transport the suspension in Step 3 to a dissociation device, heat it, and through ultrasonic vibration and dissociation vibration sound waves, disperse and dissociate the particles to generate small particles;

[0010] Step 5: Grind and screen the dissociated particles through a drum sieve to make them reach the required size.

[0011] Further, the treatment of the plant raw materials in Step 1 includes drying and pulverizing, and uniformly mixing. The pulverized plant raw materials are added to water and soaked at 40-60 °C for 1-2 hours, then fished out, dried, and reserved for use. Ethanol extraction is carried out and repeated multiple times to combine the ethanol extracts. The ethanol extracts are spray-dried to obtain a traditional Chinese medicine extract.

[0012] Further, in Step 2, the weight ratio of Beauveria bassiana, corn flour, and biochar is 1:5:100, and they are stirred evenly to obtain a colorless or slightly colored and smooth-surfaced powdered drug mixture.

[0013] Further, the plant raw materials include the following components: Omphalia lapidescens, Celastrus angulatus, Quisqualis indica, Areca catechu, Tetrapanax papyrifer, Aucklandia lappa, Sophora japonica, Perilla frutescens, Sophora flavescens, Stemona japonica, Artemisia annua. The weight ratio of each component of the plant raw materials is: Omphalia lapidescens 8-12 parts, Celastrus angulatus 30-50 parts, Aucklandia lappa 8-12 parts, Sophora japonica 5-15 parts, Perilla frutescens 12-20 parts, Sophora flavescens 20-30 parts, Stemona japonica 6-8 parts, Artemisia annua 20-24 parts.

[0014] Further, the weight ratio of each component of the plant raw materials is: Omphalia lapidescens 8 parts, Celastrus angulatus 30 parts, Aucklandia lappa 8 parts, Sophora japonica 5 parts, Perilla frutescens 12 parts, Sophora flavescens 20 parts, Stemona japonica 6 parts, Artemisia annua 20 parts.

[0015] Further, the weight ratio of each component of the plant raw materials is: Omphalia lapidescens 10 parts, Celastrus angulatus 40 parts, Aucklandia lappa 10 parts, Sophora japonica 10 parts, Perilla frutescens 16 parts, Sophora flavescens 25 parts, Stemona japonica 7 parts, Artemisia annua 22 parts.

[0016] Further, the weight ratio of each component of the plant raw materials is: Omphalia lapidescens 12 parts, Celastrus angulatus 50 parts, Aucklandia lappa 12 parts, Sophora japonica 15 parts, Perilla frutescens 20 parts, Sophora flavescens 30 parts, Stemona japonica 8 parts, Artemisia annua 24 parts.

[0017] Further, the auxiliary agents include the following components: antioxidants, preservatives, stabilizers, synergists, protectants, and solvents.

[0018] Further, the antioxidant uses one or more of vitamin E or tert-butylhydroxytoluene, the preservative uses one or more of sodium benzoate or potassium sorbate; the stabilizer uses one or more of xanthan gum or carboxymethyl cellulose, the protectant uses one or more of silica or mica powder, and the solvent uses one or more of water, ethanol, or acetone.

[0019] Further, the weight ratio of each component of the auxiliary agents is: antioxidant:preservative:stabilizer:synergist:protectant:solvent = 4:3:2:5:6:50.

[0020] The advantages of the present invention compared with the existing technology are as follows:

[0021] 1. The biological agent of the present invention can effectively control the number of pests, reduce the damage of pests to crops, thereby improving the yield and quality of agricultural crops and having no impact on non-target organisms, which is conducive to protecting the ecological environment and realizing the sustainable development of agriculture.

[0022] 2. The antioxidant of the present invention can prevent the active ingredients in the biological pesticide from degrading due to oxidation, thereby extending its effective period. The preservative is used to inhibit the growth of microorganisms and prevent the biological pesticide from being contaminated by bacteria or fungi during storage. The stabilizer helps to maintain the physical and chemical stability of the biological pesticide, and the protective agent is used to protect the active ingredients in the biological pesticide and prevent them from decomposing under light or high-temperature conditions.

[0023] 3. In the research of the carrier of the present invention, by measuring the activity of Beauveria bassiana at different dosages on different substrates, different concentration gradients are set for the optimal concentration and the carrier respectively, and the optimal carrier is determined by measuring the germination rate of Beauveria bassiana. Detailed implementation mode

[0024] In order to make the content of the present invention easier to be clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention.

[0025] Embodiment 1

[0026] A preparation method of a long-acting insecticidal biological agent, comprising the following steps:

[0027] Step 1: Prepare plant raw materials, process and extract to obtain a traditional Chinese medicine extract. The weight ratios of the components of the plant raw materials are as follows: Omphalia lapidescens 8 parts, Celastrus angulatus 30 parts, Aucklandia lappa 8 parts, Sophora japonica 5 parts, Perilla frutescens 12 parts, Sophora flavescens 20 parts, Stemona japonica 6 parts, Artemisia annua 20 parts.

[0028] The treatment of plant raw materials includes drying, pulverizing, and uniformly mixing. The pulverized plant raw materials are added to water and soaked at 40 °C for 1 hour, then fished out and dried for later use; ethanol extraction is carried out and repeated multiple times to combine the ethanol extracts; the ethanol extract is spray-dried to obtain a traditional Chinese medicine extract.

[0029] Step 2: Mix and configure the traditional Chinese medicine extract in Step 1, and at the same time add Beauveria bassiana, corn starch, biochar and additives. The weight ratio of Beauveria bassiana, corn starch and biochar is 1:5:100, and they are stirred evenly to obtain a colorless or slightly colored and smooth-surfaced drug powder mixture. The additives include the following components: antioxidant, preservative, stabilizer, synergist, protective agent and solvent. The weight ratios of the components of the additives are as follows: oxidant: preservative: stabilizer: synergist: protective agent: solvent = 4:3:2:5:6:50. The traditional Chinese medicine extract and the additives are added in sequence and stirred evenly.

[0030] The oxidant uses one or more of vitamin E or tert-butylhydroxytoluene, the preservative uses one or more of sodium benzoate or potassium sorbate; the stabilizer uses one or more of xanthan gum or carboxymethyl cellulose, the protective agent uses one or more of silica or mica powder, and the solvent uses one or more of water, ethanol, or acetone.

[0031] Antioxidants can prevent the active ingredients in biological pesticides from degrading due to oxidation, thereby extending their effective period. Preservatives are used to inhibit the growth of microorganisms and prevent biological pesticides from being contaminated by bacteria or fungi during storage. Stabilizers help maintain the physical and chemical stability of biological pesticides and prevent them from stratifying or precipitating. Synergists can enhance the effect of biological pesticides, enabling them to exert good pesticidal effects even at low doses. Protective agents are used to protect the active ingredients in biological pesticides and prevent them from decomposing under light or high-temperature conditions. Solvents are used to dissolve and disperse the active ingredients in biological pesticides to ensure uniform distribution during application.

[0032] Step 3: After uniformly mixing the mixture in Step 2, add a certain amount of water, stir evenly, and at a temperature of 60 °C, feed the mixture into a drug suspension machine. After stirring and suspension, a suspension is formed.

[0033] Step 4: Transport the suspension in Step 3 to a dissociation device, heat it, and through ultrasonic vibration and dissociation vibration sound waves, disperse and dissociate the particles to generate small particles.

[0034] Step 5: Grind and screen the dissociated particles through a drum sieve to make them reach the required size.

[0035] Comparative Example 1

[0036] A preparation method of a long-acting pesticidal biological agent includes the following steps:

[0037] Step 1: Prepare plant raw materials, process and extract to obtain a traditional Chinese medicine extract. The weight ratios of each component of the plant raw materials are: 10 parts of Omphalia lapidescens, 40 parts of Celastrus angulatus, 10 parts of Aucklandia lappa, 10 parts of Sophora japonica, 16 parts of Perilla frutescens, 25 parts of Sophora flavescens, 7 parts of Stemona japonica, and 22 parts of Artemisia annua.

[0038] The treatment of plant raw materials includes drying and pulverizing, and uniformly mixing. Add the pulverized plant raw materials to water, soak at 50 degrees Celsius for 1.5 hours, fish out and dry for later use; perform alcohol extraction with ethanol, repeat multiple times to combine the alcohol extracts; perform spray drying on the alcohol extracts to obtain a traditional Chinese medicine extract.

[0039] Step 2: Mix and configure the traditional Chinese medicine extract obtained in Step 1. At the same time, add Beauveria bassiana, corn flour, biochar, and additives. The weight ratio of Beauveria bassiana, corn flour, and biochar is 1:5:100, and stir evenly to obtain a colorless or slightly colored and smooth-surfaced powdered drug mixture. The additives include the following components: antioxidant, preservative, stabilizer, synergist, protectant, and solvent. The weight ratio of each component of the additives is antioxidant:preservative:stabilizer:synergist:protectant:solvent = 4:3:2:5:6:50. Add the traditional Chinese medicine extract and the additives in sequence and stir evenly.

[0040] The antioxidant uses one or more of vitamin E or tert-butylhydroxytoluene; the preservative uses one or more of sodium benzoate or potassium sorbate; the stabilizer uses one or more of xanthan gum or carboxymethyl cellulose; the protectant uses one or more of silica or mica powder; the solvent uses one or more of water, ethanol, or acetone.

[0041] Step 3: After uniformly mixing the mixture in Step 2, add a certain amount of water and stir evenly. At a temperature of 70 °C, feed the mixture into a drug suspension machine, and after stirring and suspending, form a suspension.

[0042] Step 4: Transport the suspension in Step 3 to a dissociation device, heat it, and through ultrasonic vibration and dissociation vibration sound waves, disperse and dissociate the particles to generate small particles.

[0043] Step 5: Grind and screen the dissociated particles through a drum sieve to make them reach the required size.

[0044] A long-acting insecticidal biological agent will be subjected to a field test. Divide the test field into 6 plots of 1×1, with a 3-meter interval between each small plot. Place an insertion sign in each small plot, and mark the treatment name on the insertion sign; mix the first three small plots into the field at a dosage of 50 g of Beauveria bassiana granule per square meter; plant soybeans in all small plots, and after they mature, introduce an equal amount of Holotrichia larvae; after introducing the larvae, sprinkle 50 g of Beauveria bassiana granule per square meter into the field; when the Holotrichia starts to die, start counting the number of live insects in each small plot. Find a suitable application method.

[0045] Comparative Example 2

[0046] A preparation method of a long-acting insecticidal biological agent, comprising the following steps:

[0047] Step 1: Prepare plant raw materials, process and extract to obtain a traditional Chinese medicine extract. The weight ratio of each component of the plant raw materials is: Omphalia lapidescens 12 parts, Celastrus angulatus 50 parts, Aucklandia lappa 12 parts, Sophora japonica 15 parts, Perilla frutescens 20 parts, Sophora flavescens 30 parts, Stemona japonica 8 parts, Artemisia annua 24 parts.

[0048] The processing of plant raw materials includes drying and pulverizing, and then uniformly mixing. The pulverized plant raw materials are added to water and soaked at 60 °C for 2 hours, and then fished out and dried for standby; ethanol extraction is carried out and repeated multiple times to combine the ethanol extracts; the ethanol extracts are spray-dried to obtain a traditional Chinese medicine extract.

[0049] Step 2: Mix and configure the traditional Chinese medicine extract in Step 1, and at the same time add Beauveria bassiana, corn starch, biochar and an auxiliary agent. The weight ratio of Beauveria bassiana, corn starch and biochar is 1:5:100, and they are stirred evenly to obtain a colorless or slightly colored and smooth-surfaced powdered drug mixture. The auxiliary agent includes the following components: antioxidant, preservative, stabilizer, synergist, protective agent and solvent. The weight ratio of each component of the auxiliary agent is antioxidant:preservative:stabilizer:synergist:protective agent:solvent = 4:3:2:5:6:50. The traditional Chinese medicine extract and the auxiliary agent are added in sequence and stirred evenly.

[0050] The antioxidant uses one or more of vitamin E or tert-butylhydroxytoluene; the preservative uses one or more of sodium benzoate or potassium sorbate; the stabilizer uses one or more of xanthan gum or carboxymethyl cellulose; the protective agent uses one or more of silicon dioxide or mica powder; the solvent uses one or more of water, ethanol, and acetone.

[0051] Step 3: After uniformly mixing the mixture in Step 2, add a certain amount of water and stir evenly. At a temperature of 80 °C, the mixture is fed into a drug suspension machine, and after stirring and suspension, a suspension is formed.

[0052] Step 4: Transport the suspension in Step 3 to a dissociation device, heat it, and through ultrasonic vibration and dissociation vibration sound waves, disperse and dissociate the particles to generate small particles.

[0053] Step 5: Grind and screen the dissociated particles through a drum sieve to make them reach the required size.

[0054] Example 2

[0055] The test method includes carrier research, optimal concentration determination and optimal carrier determination.

[0056] Carry out strain activation. The slant strains stored for more than 3 months need to be activated before use. Inoculate the slant-preserved strains onto the plate of the medium and culture at 26 °C for 7 - 10 days until sporulation. Add 50 mL of liquid medium into an Erlenmeyer flask (250 ml) and sterilize. Scrape a small amount of spore powder on the plate and dissolve it with 0.05% Tween-80 sterile water to make a spore suspension, and inoculate it into the sterilized liquid medium at a concentration of 10⁷ cells / mL, and shake culture (26 °C, 200 rpm, 48 h) to make a seed liquid containing spores and hyphae. After the above operations are completed, first pour 300 ml of liquid medium (35 g glucose, 15 g yeast powder, 0.037 g / L potassium chloride, 0.25 g / L magnesium sulfate monohydrate, 1.20 g / L sodium phosphate, 1 L distilled water) into a 1 L shake flask, then inoculate the prepared 10⁷ / ml spore suspension into the shake flask, and shake culture on a shaker at 150 rpm and 26 °C for 3 d. In addition, under the condition of 121 °C, use the autoclaving method to sterilize the solid substrate (wheat bran and rice husk at a ratio of 4:1, 0.3% potassium phosphate, 0.1% magnesium sulfate heptahydrate, 0.1% ammonium nitrate) for 30 min. For inoculating the solid substrate, first culture 1.5 L of spores with a spore content of 10¹¹ / ml, and then transfer them to fermentation bags (a total of 12 bags), ensuring that each fermentation sack (74 cm × 107 cm) contains approximately 3 kg of solid substrate, and place it at 26 °C for 10 days. In the first 3 d of the fermentation process, in order to promote the growth of hyphae, use a 500 w fan to work continuously for 3 d, and keep the environmental humidity above 90%. After 10 days, place the solid medium on a YC-6 spore sieve to separate the spores from the substrate.

[0057] Then, pour 300 ml of liquid medium (35 g glucose, 15 g yeast powder, 0.037 g / L potassium chloride, 0.25 g / L magnesium sulfate monohydrate, 1.20 g / L sodium phosphate, 1 L distilled water) into a 1 L shake flask, and then inoculate the prepared 10⁷ / ml spore suspension into the shake flask, and shake culture on a shaker at 150 rpm and 26 °C for 3 d. In addition, under the condition of 121 °C, use the autoclaving method to sterilize the solid substrate (wheat bran and rice husk at a ratio of 4:1, 0.3% potassium phosphate, 0.1% magnesium sulfate heptahydrate, 0.1% ammonium nitrate) for 30 min. For inoculating the solid substrate, first culture 1.5 L of spores with a spore content of 10¹¹ / ml, and then transfer them to fermentation bags (a total of 12 bags), ensuring that each fermentation sack (74 cm × 107 cm) contains approximately 3 kg of solid substrate, and place it at 26 °C for 10 days. In the first 3 d of the fermentation process, in order to promote the growth of hyphae, use a 500 w fan to work continuously for 3 d, and keep the environmental humidity above 90%. After 10 days, place the solid medium on a YC-6 spore sieve to separate the spores from the substrate.

[0058] Determine the activities of Beauveria bassiana at different dosages on different substrates. Respectively use 400 g, 450 g, 500 g, 550 g, 600 g / mu of Beauveria bassiana. Among them, the particle size of biochar should be greater than 120 mesh, and the particle size of corn flour should be greater than 200 mesh. Mix different carriers with biochar, corn flour, and Beauveria bassiana in the same proportion. (Ratio: Beauveria bassiana: biochar: corn flour = 1:2000:100) Inoculate different concentrations of the mixture onto Sabouraud dextrose agar medium (SDAY) respectively, culture for seven days to obtain conidia, and scrape the conidia on the upper surface of the SDAY plate with a sterile inoculation stick to obtain the optimal concentration ratio.

[0059] Set gradients of the optimal concentration and the substrate at ratios of 1:1000, 1:750, 1:500, 1:250, 1:100, and 1:50, and mix them with attapulgite, bentonite, kaolin, diatomite, and vermiculite respectively, then inoculate them into the medium. Measure their germination rates every week for the first three weeks and then every month. Select the most suitable ratio.

[0060] Example 3

[0061] The biochar and corn flour were respectively sieved through 60 mesh, 80 mesh, 100 mesh, 120 mesh, 140 mesh, 160 mesh, 180 mesh and 200 mesh sieves, and the optimal mesh number and mixing ratio of the combination of biochar, corn flour and Beauveria bassiana were obtained through preliminary pre-tests.

[0062] The following table shows different substrate formulations

[0063]

[0064]

[0065] Determination of sporulation amount: When each treatment was cultured for 10 days and one month, an appropriate amount of each treatment was taken, and a spore suspension was prepared with sterile water. Observation and counting were carried out under a microscope using a hemocytometer to calculate the spore concentration.

[0066] Spore germination rate: An appropriate amount of each treatment was taken to prepare a spore suspension of 0.1 g / mL, and it was placed under a microscope to observe and record the germination of conidia. The above operations were all carried out under sterile conditions.

[0067] The test results showed that the treatment of biochar + corn flour + fungus had the highest sporulation amount and germination rate. When the treatment time was the same, there were significant differences in the number of spores and germination rate among different treatments (number of spores: at 10 days: F(5,23) = 166.016, P < 0.05, at 1 month: F(5,23) = 628.605, P < 0.05; germination rate: at 10 days: F(5,23) = 5043.339, P < 0.05, at 1 month: F(5,23) = 6583.879, P < 0.05).

[0068] The following table shows the analysis and comparison of the sporulation amount and spore germination rate of Beauveria bassiana on different carriers

[0069]

[0070] The above describes the present invention and its implementation manners. Such description is not restrictive, and what is shown is only one of the implementation manners of the present invention. Generally speaking, if those of ordinary skill in the art are inspired by it and, without departing from the gist of the present invention, design similar structural modes and embodiments to this technical solution without creative efforts, they shall fall within the protection scope of the present invention.

Claims

1. A preparation method of a long-acting insecticidal biological agent, characterized in that: It includes the following steps; Step 1: Prepare plant raw materials, process and extract to obtain a traditional Chinese medicine extract; Step 2: Mix and configure the traditional Chinese medicine extract in Step 1, and simultaneously add Beauveria bassiana, corn starch, biochar and additives; Step 3: After uniformly mixing the mixture in Step 2, add a certain amount of water, stir evenly, and at a temperature of 60°C - 80°C, feed the mixture into a drug suspension machine, and through stirring and suspension, form a suspension; Step 4: Transport the suspension in Step 3 to a dissociation device, heat it, and through ultrasonic vibration and dissociation vibration sound waves, disperse and dissociate the particles to generate small particles; Step 5: Grind and screen the dissociated particles through a drum sieve to make them reach the required size.

2. The long-acting insecticidal biological agent according to claim 1, wherein: The treatment of plant raw materials in Step 1 includes drying and pulverizing, and uniformly mixing. Add the pulverized plant raw materials into water, soak them at 40 - 60 degrees Celsius for 1 - 2 hours, fish them out and dry them for standby; perform alcohol extraction with ethanol, repeat multiple times to combine the alcohol extracts; Perform spray drying on the alcohol extract to obtain a traditional Chinese medicine extract.

3. The long-acting insecticidal biological agent according to claim 1, characterized in that: In Step 2, the weight ratio of Beauveria bassiana, corn starch, and biochar is 1:5:100, and stir evenly to obtain a colorless or slightly colored and smooth-surfaced drug powder mixture.

4. The preparation method of a long-acting insecticidal biological agent according to claim 1, characterized in that: The plant raw materials include the following components: Omphalia lapidescens, Celastrus angulatus, Quisqualis indica, Areca catechu, Tetrapanax papyrifer, Aucklandia lappa, Sophora japonica, Perilla frutescens, Sophora flavescens, Stemona japonica, Artemisia annua. The weight ratio of each component of the plant raw materials is: Omphalia lapidescens 8 - 12 parts, Celastrus angulatus 30 - 50 parts, Aucklandia lappa 8 - 12 parts, Sophora japonica 5 - 15 parts, Perilla frutescens 12 - 20 parts, Sophora flavescens 20 - 30 parts, Stemona japonica 6 - 8 parts, Artemisia annua 20 - 24 parts.

5. The preparation method of a long-acting insecticidal biological agent according to claim 4, characterized in that: The weight ratio of each component of the plant raw materials is: Omphalia lapidescens 8 parts, Celastrus angulatus 30 parts, Aucklandia lappa 8 parts, Sophora japonica 5 parts, Perilla frutescens 12 parts, Sophora flavescens 20 parts, Stemona japonica 6 parts, Artemisia annua 20 parts.

6. The preparation method of a long-acting insecticidal biological agent according to claim 4, characterized in that: The weight ratio of each component of the plant raw materials is: Omphalia lapidescens 10 parts, Celastrus angulatus 40 parts, Aucklandia lappa 10 parts, Sophora japonica 10 parts, Perilla frutescens 16 parts, Sophora flavescens 25 parts, Stemona japonica 7 parts, Artemisia annua 22 parts.

7. The preparation method of a long-acting insecticidal biological agent according to claim 4, characterized in that: The weight ratio of each component of the plant raw materials is: Omphalia lapidescens 12 parts, Celastrus angulatus 50 parts, Aucklandia lappa 12 parts, Sophora japonica 15 parts, Perilla frutescens 20 parts, Sophora flavescens 30 parts, Stemona japonica 8 parts, Artemisia annua 24 parts.

8. The preparation method of a long-acting insecticidal biological agent according to claim 1, characterized in that: The additives include the following components: antioxidant, preservative, stabilizer, synergist, protective agent and solvent.

9. The preparation method of a long-acting insecticidal biological agent according to claim 8, wherein, The antioxidant uses one or more of vitamin E or tert-butylhydroxytoluene, the preservative uses one or more of sodium benzoate or potassium sorbate; the stabilizer uses one or more of xanthan gum or carboxymethyl cellulose, the protective agent uses one or more of silica or mica powder, and the solvent uses one or more of water, ethanol, and acetone.

10. The preparation method of a long-acting insecticidal biological agent according to claim 8, characterized in that, The weight ratio of each component of the additives is: antioxidant: preservative: stabilizer: synergist: protective agent: solvent = 4:3:2:5:6:50.

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