Fullerene-containing composition with wrinkle fading and brightening effects as well as preparation method and application of fullerene-containing composition

Abstract

The invention discloses a fullerene-containing wrinkle fading and brightening composition as well as a preparation method and application thereof, and particularly belongs to the field of skin care products. The wrinkle fading and brightening composition containing fullerene is prepared from the following components in parts by mass: 1 to 5 parts of water-soluble fullerene, 0.1 to 0.8 part of 4-butylresorcinol and 4 to 10 parts of plant extraction component, wherein the plant extraction component is prepared by compounding peony root extract, amur corktree bark extract and white willow bark extract. The water-soluble fullerene, the 4-butylresorcinol, the peony root extract, the amur corktree bark extract and the white willow bark extract are matched for use, so that the activity of elastase can be effectively inhibited, DPPH free radicals are removed, and meanwhile, the components have an efficient promoting effect on regeneration of skin collagen. The skin lingering agent containing the wrinkle fading and brightening composition provided by the invention has excellent efficacy in the aspects of tightening skin, reducing wrinkles and whitening and brightening skin color.

Description

Technical Field

[0001] The invention belongs to the field of skin care products, and particularly relates to a wrinkle-lightening and brightening composition containing fullerene, and a preparation method and application thereof. Background Art

[0002] As the largest organ of the human body, the skin is in direct contact with the external environment and is easily stimulated by external factors such as oxidation, dryness, and ultraviolet radiation. It is also affected by internal factors such as aging, eating habits, and lifestyle, which lead to the gradual decline of skin function and the emergence of problems such as dryness, roughness, wrinkles, dullness, and sagging, which seriously affect personal image and self-confidence. With the improvement of living standards and the increase in attention to their own image, consumers are increasingly in need of cosmetics that can effectively improve skin conditions, especially products with the effect of lightening and brightening, which have become a hot demand in the market. However, most of the existing products for lightening and brightening on the market have problems such as insignificant effects and single mechanism of action, which are difficult to meet consumers' expectations for efficient and comprehensive skin care. Summary of the invention

[0003] In view of the deficiencies of the prior art, the purpose of the present invention is to provide a wrinkle-lightening and brightening composition containing fullerene, which improves skin condition from multiple angles through the synergistic effect of multiple active ingredients and meets consumers' demand for high-quality cosmetics.

[0004] In order to achieve the above object, the present invention discloses the following technical solutions:

[0005] In a first aspect, the present invention provides a wrinkle lightening and brightening composition containing fullerene, wherein the composition comprises the following components in parts by mass:

[0006] 1-5 parts of water-soluble fullerene;

[0007] 4-Butylresorcinol 0.1-0.8 parts;

[0008] 4-10 parts of plant extract components;

[0009] The plant extract component is prepared by compounding peony root extract, phellodendron amurense bark extract and willow bark extract.

[0010] Water-soluble fullerene, 4-butylresorcinol, peony root extract, phellodendron bark extract and white willow bark extract each have unique benefits in the formula, and when used together, they have a significant effect on reducing wrinkles and brightening the skin.

[0011] Water-soluble fullerene has strong antioxidant capacity, can remove free radicals, reduce skin oxidative damage, prevent wrinkles and brighten skin tone, while promoting collagen production and enhancing skin elasticity;

[0012] 4-Butylresorcinol blocks melanogenesis by inhibiting the activities of tyrosinase and TRP-1, reduces pigment deposition, and achieves the effect of whitening and lightening spots;

[0013] The extract of Paeonia suffruticosa root is rich in various active ingredients, with effects such as antioxidant, anti-inflammatory, moisturizing, and inhibiting pigment deposition. It can improve uneven skin tone, make the skin brighter and more uniform, and at the same time promote collagen production and enhance skin firmness;

[0014] The extract of Phellodendron amurense bark has antibacterial, anti-inflammatory, and antioxidant effects. It can soothe the skin, reduce inflammation, reduce pigmentation, and maintain skin health;

[0015] The extract of Salix alba bark has moisturizing and antioxidant effects. It can eliminate free radicals, protect the skin, and at the same time promote collagen production and improve skin firmness.

[0016] The research of this invention finds that these above-mentioned ingredients synergistically act in reducing wrinkles and brightening the skin. Water-soluble fullerene, the extract of Paeonia suffruticosa root, the extract of Phellodendron amurense bark, and the extract of Salix alba bark jointly have antioxidant effects and enhance the skin's ability to resist free radicals; 4-Butylresorcinol and the extract of Paeonia suffruticosa root cooperate to inhibit melanogenesis and brighten the skin tone; the extract of Phellodendron amurense bark, the extract of Paeonia suffruticosa root, and the extract of Salix alba bark jointly play anti-inflammatory and soothing roles, reduce pigmentation, promote collagen production, and inhibit elastase activity; generally speaking, these ingredients improve the skin condition from multiple perspectives, reduce wrinkles, brighten the skin tone, inhibit pigment deposition, and soothe inflammation, achieving a comprehensive effect of reducing wrinkles and brightening the skin.

[0017] Preferably, by mass parts, the composition comprises the following components:

[0018] Water-soluble fullerene 2 - 4 parts;

[0019] 4-Butylresorcinol 0.1 - 0.3 parts;

[0020] Plant extract component 5 - 7 parts;

[0021] The plant extract component is compounded from the extract of Paeonia suffruticosa root, the extract of Phellodendron amurense bark, and the extract of Salix alba bark according to a mass ratio of 1:(3 - 6):(1 - 2).

[0022] Further preferably, by mass parts, the composition comprises the following components:

[0023] Water-soluble fullerene 3 parts;

[0024] 4-Butylresorcinol 0.2 parts;

[0025] Plant extract component 6 parts.

[0026] In a second aspect, the present invention provides a skin-retaining agent having the effects of reducing wrinkles and brightening skin tone, which comprises the wrinkle-reducing and skin-brightening composition containing fullerenes described in the first aspect.

[0027] Preferably, the addition amount of the wrinkle-reducing and skin-brightening composition containing fullerenes in the skin-retaining agent is 1-3 wt%.

[0028] More preferably, the skin-retaining agent further comprises a base oil, an emulsifier, a preservative, a humectant, a pH regulator, and a solvent.

[0029] Even more preferably, the base oil is at least one of olive fruit oil and jojoba seed oil;

[0030] The emulsifier is at least one of lauryl polyether-23, polysorbate-80, and hydroxyethyl cellulose;

[0031] The humectant is at least one of glycerol, sodium hyaluronate, dipropylene glycol, and 1,2-pentanediol;

[0032] The preservative is at least one of p-hydroxyacetophenone and phenoxyethanol;

[0033] The pH regulator is at least one of arginine, potassium hydroxide, citric acid, and sodium citrate;

[0034] The solvent is deionized water or pure water.

[0035] In a third aspect, the present invention provides a preparation method of the skin-retaining agent having the effects of reducing wrinkles and brightening skin tone described in the second aspect, comprising the following steps:

[0036] Step 1. Stir and mix the base oil and the emulsifier evenly to obtain solution A;

[0037] Step 2. Stir and mix 4-butylresorcinol, the preservative, 1,2-pentanediol, glycerol, and dipropylene glycol evenly to obtain solution B;

[0038] Step 3. Stir and mix water-soluble fullerenes, plant extract components, sodium hyaluronate, and deionized water evenly to obtain solution C;

[0039] Step 4. Mix solution A, solution B, and solution C, perform high-speed homogenization and dispersion using a homogenizer, and add a pH regulator to adjust the pH value to 6.5-7 to obtain the skin-retaining agent.

[0040] In a fourth aspect, the present invention provides the application of the wrinkle-reducing and skin-brightening composition containing fullerenes described in the first aspect in the preparation of skin care products having the effects of reducing wrinkles, firming skin, anti-wrinkle, anti-aging, whitening, and / or lightening spots.

[0041] Advantages of the present invention:

[0042] 1. The present invention combines water-soluble fullerene, 4-butylresorcinol, peony root extract, phellodendron bark extract and white willow bark extract, which can effectively inhibit the activity of elastase, scavenge DPPH free radicals, and have a highly efficient promoting effect on the regeneration of skin collagen;

[0043] 2. The skin retainer containing the anti-wrinkle and brightening composition provided by the present invention has excellent efficacy in tightening the skin, reducing wrinkles, and whitening and brightening the skin tone. Description of the Drawings

[0044] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below with reference to the drawings.

[0045] Figure 1 It is a diagram showing the change of crow's feet of Subject No. 20 after using the product for 28 days.

[0046] Specific Embodiments

[0047] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0048] Unless otherwise specified, the test methods used in the examples and comparative examples are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, can all be obtained from commercial sources; the percentages mentioned in the examples and comparative examples, unless otherwise specified, are all mass percentages.

[0049] In the present invention:

[0050] Water-soluble fullerene: purchased from Guangzhou Baihaobo Co., Ltd.;

[0051] 4-Butylresorcinol: purchased from Zhuanglai Holding Group Co., Ltd.;

[0052] Peony root extract: purchased from Shanghai Ruidian Biotechnology Co., Ltd.;

[0053] Phellodendron bark extract: purchased from Guangzhou Jizhou Trading Co., Ltd.;

[0054] White willow bark extract: purchased from Guangzhou Jizhou Trading Co., Ltd.

[0055] Preparation of the plant extract component:

[0056] Accurately weigh each raw material according to the mass ratio of each raw material in Table 1, and place each raw material in a high-speed blender and stir evenly to obtain the plant extract component.

[0057] Table 1 Raw materials and their mass ratios in the plant extract components

[0058] Raw material Component ① Component ② Component ③ Component ④ Component ⑤ Component ⑥ Component ⑦ Component ⑧ Paeonia root extract 1 1 1 - 1 1 1 4.5 Phellodendron bark extract 3 4.5 6 4.5 - 4.5 1.5 1 White willow bark extract 1 1.5 2 1.5 1.5 - 4.5 1.5

[0059] Note: "-" in the table indicates no addition.

[0060] Preparation of skin retention agent

[0061] Accurately weigh each component according to the dosage ratio of each component in Tables 2, 3, and 4;

[0062] Step 1. At 90 °C, stir and mix the base oils evenly at 500 r / min. After cooling to 50 °C, add the emulsifier and stir and mix evenly at 200 r / min to obtain Solution A;

[0063] Step 2. Stir and mix 4-butylresorcinol, preservative, 1,2-pentanediol, glycerol, and dipropylene glycol evenly at 30 °C and 500 r / min to obtain Solution B;

[0064] Step 3. Stir and mix water-soluble fullerene, plant extract components, sodium hyaluronate, and deionized water evenly at 30 °C and 500 r / min to obtain Solution C;

[0065] Step 4. Mix Solution A, Solution B, and Solution C, and use a homogenizer to perform high-speed homogenization and dispersion for 20 min. Add a pH regulator to adjust the pH value to 7 to obtain the skin retention agent.

[0066] Table 2 Components and their mass ratios of the anti-wrinkle and brightening composition

[0067]

[0068] Table 3 Components and their mass percentages of the skin retention agent

[0069]

[0070]

[0071] Table 4 Components and their mass percentages of the comparative skin retention agent

[0072]

[0073] Note: "-" in the table indicates no addition.

[0074] Performance test

[0075] 1 In vitro test

[0076] 1.1 Inhibition test of elastase

[0077] Sample group: 10 μL of the skin retention agents prepared in Examples 1-5 and Comparative Examples 1-7 were respectively taken and added into a 96-well plate, then 20 μL of elastase (porcine pancreas) solution with a concentration of 0.1 U / mL was added. After incubation in a constant temperature incubator at 25 °C for 15 min, 50 μL of a substrate (N-succinyl-L-alanyl-L-alanine with a purity of 98%) with a concentration of 1 mg / mL was added. Finally, the absorbance value A of the solution at 410 nm was measured using an enzyme-linked immunosorbent assay (ELISA) reader. X . Three replicate wells were set, and the results were averaged.

[0078] Control group: 10 μL of deionized water was taken and added into a 96-well plate, then 20 μL of elastase (porcine pancreas) solution with a concentration of 0.1 U / mL was added. After incubation in a constant temperature incubator at 25 °C for 15 min, 50 μL of a substrate (N-succinyl-L-alanyl-L-alanine with a purity of 98%) with a concentration of 1 mg / mL was added. Finally, the absorbance value A of the solution at 410 nm was measured using an enzyme-linked immunosorbent assay (ELISA) reader. O . Three replicate wells were set, and the results were averaged.

[0079] The results were obtained according to the following elastase inhibition rate calculation formula, and the results are shown in Table 5.

[0080] Inhibition rate (%) = [(A O - A X ) / A O × 100%

[0081] Table 5 Results of porcine pancreas elastase inhibition rate

[0082] Group Elastase inhibition rate / % Example 1 73.12 Example 2 75.68 Example 3 79.13 Example 4 77.35 Example 5 74.08 Comparative Example 1 58.64 Comparative Example 2 61.42 Comparative Example 3 57.86 Comparative Example 4 64.47 Comparative Example 5 67.93 Comparative Example 6 42.61 Comparative Example 7 39.53

[0083] 1.2 DPPH-radical scavenging test

[0084] According to the method specified in T / SHRH 006-2018, the DPPH radical scavenging rates (%) of the skin retention agents prepared in Examples 1-5 and Comparative Examples 1-7 above were respectively detected. The higher the DPPH radical scavenging rate, the better the anti-wrinkle effect of the skin retention agent. The results are shown in Table 6.

[0085] Table 6 DPPH-radical scavenging rate

[0086]

[0087]

[0088] 1.3 Type I collagen synthesis promotion test

[0089] In this experiment, fibroblasts were used as the detection object. A cell photoaging damage model was established by UVA irradiation, and the change in the content of type I collagen (Collagen I) after the action of the sample to be tested was detected to evaluate the firming and anti-wrinkle efficacy of the sample to be tested.

[0090] (1) Seeding: Cells were seeded into 96-well plates at an inoculation density of 1×10 5 cells / well and placed in an incubator overnight (37 °C, 5 v / v% CO2).

[0091] (2) UVA irradiation: After washing the cells with PBS, according to the experimental grouping in Table 7, the groups with UVA irradiation were irradiated with 30 J / cm 2 of UVA.

[0092] (3) Solution preparation: The skin-retaining agents prepared in Examples 1-5 and Comparative Examples 1-7 were selected as the sample solutions to be tested, and TGF-β1 (100 ng / mL) was used as the positive control for the experiment. 100 μL of sample was added to each well, and 3 replicates were set for each group. After dosing, the 96-well plates were placed in an incubator at 37 °C and incubated in the dark for 24 h.

[0093] Table 7 Experimental grouping

[0094]

[0095] (4) Collection of cell supernatant: After 24 h of dosing, the 96-well plates were taken out, and the cell supernatants corresponding to each sample group were collected into centrifuge tubes, centrifuged at 1000 rpm for 10 min, and the supernatants were collected and placed in 1.5 mL centrifuge tubes for storage at -20 °C for later use.

[0096] (5) Determination of the concentration of type I collagen in the cell supernatant by ELISA kit: The kit and the sample to be tested were taken out 30 min before the test, placed at room temperature and then used. The test operation was carried out strictly according to the kit instructions.

[0097] Table 8 Detection results of type I collagen content

[0098] Sample name Average value of type I collagen content (ng / mL) Growth rate of type I collagen (%) Model control 1.329 / Blank (not damaged by UVA) 1.671 25.73 Positive (TGFβ1 100 ng / mL) 1.595 20.02 Example 1 1.801 35.52 Example 2 1.833 37.92 Example 3 1.874 41.01 Example 4 1.859 39.88 Example 5 1.822 37.10 Comparative Example 1 1.608 20.99 Comparative Example 2 1.591 19.71 Comparative Example 3 1.625 22.27 Comparative Example 4 1.773 33.41 Comparative Example 5 1.745 31.30 Comparative Example 6 1.483 11.59 Comparative Example 7 1.511 13.69

[0099] Result analysis:

[0100] According to the results in Table 5-8, the skin-retaining agent provided by the present invention has excellent effects of firming and anti-wrinkle. Among them, the anti-wrinkle and brightening compositions used in Comparative Examples 1-7 are based on the formula of the anti-spot and brightening composition used in Example 3, with key components being defaulted and the mass ratios between key components being adjusted. The anti-wrinkle and brightening composition in Comparative Example 1 lacks the peony root extract, resulting in a significant decline in its elastase inhibitory performance, free radical scavenging performance, and promoting performance on collagen. The anti-wrinkle and brightening compositions in Comparative Examples 2 and 3 lack the phellodendron bark extract and white willow bark extract respectively, and similarly show results similar to those in Comparative Example 1, proving the key role of the peony root extract, phellodendron bark extract, and white willow bark extract in the formula. The mass ratios of the peony root extract, phellodendron bark extract, and white willow bark extract in the anti-wrinkle and brightening compositions used in Comparative Examples 4-5 are not within the mass ratio range defined by the present invention, and their elastase inhibitory performance, free radical scavenging performance, and promoting performance on collagen also show a significant decline compared to Example 3, proving the key role of the specific dosage ratio of the peony root extract, phellodendron bark extract, and white willow bark extract in the overall formula. The anti-spot compositions used in Comparative Examples 6-7 lack water-soluble fullerene and plant extract components respectively, resulting in a significant decline in their elastase inhibitory performance, free radical scavenging performance, and promoting performance on collagen, proving that water-soluble fullerene, 4-butylresorcinol, and plant extract components also play an indispensable key role in the formula.

[0101] 2 Human trials

[0102] Wrinkles reduction test

[0103] Sixty volunteers with obvious crow's feet were selected as the test subjects, numbered 1-60, and then randomly divided into 6 groups of 10 people each. Among them, the skin-retaining agents of Examples 1-5 were applied to the faces of 5 groups of test subjects, and the skin-retaining agent of the blank control group was applied to the face of 1 group of test subjects. The skin-retaining agent of the blank control group lacked the efficacy components (i.e., lacked water-soluble fullerene, 4-butylresorcinol, and plant extract components) based on the formula of Example 3, and the missing dosage was supplemented with deionized water. It was used once in the morning and once in the evening, 2 g each time, for a total of 28 days. The trial effects were detected by a skin detector, and the results are shown in Table 9 and Figure 1 , among which Figure 1 is the before-and-after comparison diagram of good improvement after 28 days of using the skin-retaining agent prepared in Example 3 by the test subject (No. 20).

[0104] Test indicators: Grade ③: Obvious crow's feet; Grade ②: Shallow crow's feet; Grade ①: No crow's feet;

[0105] Test conditions: (1) Indoor environment; (2) No direct sunlight or strong light; (3) Ambient temperature 25 ± 2°C, ambient humidity 40 - 60%.

[0106] Table 9 Results of crow's feet test

[0107]

[0108] Brightening effect test

[0109] Sixty female volunteers aged 18 - 30 were selected as the test subjects. The test subjects were required to have healthy facial skin without obvious redness, skin lesions, scars, etc. First, the test subjects were numbered from 1 to 60, and then randomly divided into 6 groups of 10 people each. Among them, the faces of 5 groups of test subjects were respectively applied with the skin-retaining agents of Examples 1 - 5, and the faces of 1 group of test subjects were applied with the skin-retaining agent of the blank control group. The skin-retaining agent of the blank control group was based on the formula of Example 3 but lacked the efficacy components (i.e., lacked water-soluble fullerene, 4-butylresorcinol, and plant extract components), and the missing amount was supplemented with deionized water. It was used once in the morning and once in the evening every day, 2 g each time, for a total of 28 days. Before applying the skin-retaining agent, the initial L* value and ITA° value of the facial skin were collected through a skin test probe. After 28 days of use, the L* value and ITA° value of the facial skin were detected. Through the formula: change rate / % = (before use - after use) / before use × 100%, the change rate of the L* value and the change rate of the ITA° value were calculated. The results of each group were averaged, and the obtained results are shown in Table 10.

[0110] Table 10 Skin brightness data

[0111] Group Change rate of L* value / % Change rate of ITA° value / % Blank control group 2.16 0.16 Example 1 10.61 2.96 Example 2 12.47 3.24 Example 3 15.26 3.98 Example 4 13.08 3.31 Example 5 12.35 3.18

[0112] Result analysis:

[0113] According to Table 9, Table 10 and Figure 1 It can be seen that the skin-retaining agent provided by the present invention has excellent efficacy performance in wrinkle reduction and brightening. By comparing with the blank control group, it can be known that the water-soluble fullerene, 4-butylresorcinol, and plant extract components provided by the present invention play a key role in the overall formula. Among them, the wrinkle reduction and brightening performance of Example 3 shows a better effect.

[0114] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A wrinkle-reducing and brightening composition containing fullerene, characterized in that, By mass parts, the composition comprises the following components: 1 - 5 parts of water - soluble fullerene; 0.1 - 0.8 part of 4 - butylresorcinol; 4 - 10 parts of plant extract component; The plant extract component is compounded from peony root extract, phellodendron bark extract and white willow bark extract.

2. The anti-wrinkle and brightening composition containing fullerenes according to claim 1, wherein By mass parts, the composition comprises the following components: 2 - 4 parts of water - soluble fullerene; 0.1 - 0.3 part of 4 - butylresorcinol; 5 - 7 parts of plant extract component; The plant extract component is compounded from peony root extract, phellodendron bark extract and white willow bark extract according to the mass ratio of 1:(3 - 6):(1 - 2).

3. The anti-wrinkle and brightening composition containing fullerenes according to claim 2, characterized in that, By mass parts, the composition comprises the following components: 3 parts of water - soluble fullerene; 0.2 part of 4 - butylresorcinol; 6 parts of plant extract component.

4. A skin - retaining agent with the efficacy of reducing fine lines and brightening, characterized in that, Containing the anti - wrinkle and brightening composition containing fullerene according to any one of claims 1 - 3.

5. The skin-remaining agent with the effect of reducing wrinkles and brightening skin according to claim 4, characterized in that, The addition amount of the anti - wrinkle and brightening composition containing fullerene is 1 - 3 wt%.

6. The skin-retaining agent with the effect of reducing wrinkles and brightening skin according to claim 5, characterized in that, The skin - retaining agent further contains base oil, emulsifier, preservative, moisturizer, pH regulator, solvent.

7. The skin-retaining agent with the effect of reducing wrinkles and brightening skin according to claim 6, characterized in that, The base oil is at least one of olive fruit oil and jojoba seed oil; The emulsifier is at least one of laureth - 23, polysorbate - 80, hydroxyethyl cellulose; The moisturizer is at least one of glycerol, sodium hyaluronate, dipropylene glycol, 1,2 - pentanediol; The preservative is at least one of p - hydroxyacetophenone and phenoxyethanol; The pH regulator is at least one of arginine, potassium hydroxide, citric acid, sodium citrate; The solvent is deionized water or pure water.

8. The preparation method of the skin-retaining agent with the efficacy of reducing fine lines and brightening skin according to claim 7, characterized in that, Comprising the following steps: Step 1. Stir and mix the base oil and the emulsifier evenly to obtain solution A; Step 2. Stir and mix 4 - butylresorcinol, preservative, 1,2 - pentanediol, glycerol, dipropylene glycol evenly to obtain solution B; Step 3. Stir and mix water - soluble fullerene, plant extract component, sodium hyaluronate and deionized water evenly to obtain solution C; Step 4. Mix solution A, solution B and solution C, perform high - speed homogenization and dispersion using a homogenizer, and add a pH regulator to adjust the pH value to 6.5 - 7 to obtain the skin - retaining agent.

9. Use of the anti - wrinkle and brightening composition containing fullerene according to any one of claims 1 - 3 in the preparation of a skin - care product having the effects of anti - wrinkle, firming, anti - wrinkle, anti - aging, whitening and / or lightening spots.

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