Composition for enhancing antioxidant effect of PQQ, combination bag of application of composition and cosmetics and cosmetics

Abstract

The invention relates to the technical field of cosmetics, in particular to a composition for enhancing the antioxidant effect of PQQ, a combination bag of application of the composition and cosmetics and the cosmetics. The invention provides a composition of cardamom seed ketone and pyrroloquinoline quinone (PQQ) or a salt thereof. The composition disclosed by the invention has good stability, and shows a remarkable synergistic effect in the aspect of oxidation resistance. In addition, a human body anti-wrinkle effect questionnaire result shows that the composition has a better anti-wrinkle effect compared with pyrroloquinoline quinone disodium salt.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition for enhancing the antioxidant effect of PQQ, its application, a combination package of cosmetics, and cosmetics. Background Art

[0002] Pyrroloquinoline quinone disodium salt (hereinafter referred to as PQQ) is a potent antioxidant with a comprehensive action pathway. The ways in which it exerts its antioxidant effect include: (1) directly scavenging free radicals and neutralizing excess ROS; (2) activating the expression of antioxidant enzymes, which can stimulate the expression and activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx), enhancing the antioxidant defense ability of cells; (3) enhancing the antioxidant signaling pathway of transcription factor Nrf2 / ARE; (4) protecting mitochondria, which can promote mitochondrial biogenesis and protect mitochondria from damage, reduce cell death, and reduce cell damage caused by mitochondrial dysfunction; (5) promoting the expression of the antioxidant enzyme heme oxygenase-1, degrading heme into biliverdin, which is a potent antioxidant that can scavenge free radicals and protect cells from oxidative damage. Biliverdin can then be converted by biliverdin reductase into bilirubin, which also has antioxidant properties; (6) indirectly promoting the activation of Nrf2 and the expression of antioxidant enzymes such as NQO1.

[0003] Although PQQ exhibits various remarkable physiological activities such as antioxidant and promoting cell growth, theoretically it has great potential in the field of cosmetics and can bring many benefits to the skin. However, the shortcoming of its poor stability seriously hinders its wide application in cosmetics. PQQ is degraded by factors such as temperature, humidity, and light, and is more likely to be degraded or discolored by other components in cosmetics, which not only leads to the loss of its active ingredients but also causes discoloration, affecting the appearance and effectiveness of cosmetics.

[0004] In view of this, on the one hand, the field has always been actively exploring substances that can synergistically interact with PQQ. By reasonably combining components with complementary effects, not only can the overall effect of PQQ be enhanced, but also the defect of its insufficient stability can be compensated to a certain extent. On the other hand, the field is still exploring solutions to ensure the stability of PQQ, with the hope of enabling PQQ to have a wider application in the field of cosmetics. Summary of the Invention

[0005] In view of this, the technical problem to be solved by the present invention is to provide a composition for enhancing the antioxidant effect of PQQ, its application, a combination package of cosmetics, and cosmetics.

[0006] The composition provided by the present invention is composed of PQQ or its salt and myristone.

[0007] The cardamom ketone is extracted from the seeds of the cardamom plant, and the active ingredient is hydroxymethoxyphenyl decanone, and the remaining ingredients are dipropylene glycol or caprylic acid / capric acid triglyceride. Experiments show that the combination of PQQ or its salt and cardamom ketone can have a good antioxidant effect, and the two can produce a significant synergistic effect at a ratio of 10:1. And according to a questionnaire survey on the anti-wrinkle efficacy of the human body, the antioxidant composition has a better anti-wrinkle effect than the disodium salt of pyrroloquinoline quinone used alone. In the present invention, the cardamom ketone and PQQ or its salt can be mixed or exist independently, and the present invention does not limit this. When the two exist in a mixed state, the state of their existence can be a solution or a powder. When the two exist independently, the state of their existence is independently selected from a solution or a powder. The present invention does not limit this either.

[0008] In the present invention, the composition consists of 0.0005 to 0.01 parts by mass of PQQ or its salt and 0.005 to 0.1 parts by mass of cardamom ketone.

[0009] Specifically, in the composition, the mass ratio of PQQ or its salt to cardamom seed ketone is 1:10.

[0010] In the present invention, the salt of PQQ is magnesium salt, sodium salt, potassium salt or calcium salt of PQQ. In the present invention, PQQ-2Na is used as an example to verify the efficacy of the composition.

[0011] More specifically, the composition consists of 0.0005 parts of PQQ-2Na and 0.005 parts of cardamom seed ketone;

[0012] or consisting of 0.001 parts of PQQ-2Na and 0.01 parts of cardamom seed ketone;

[0013] Or it is composed of 0.01 parts of PQQ-2Na and 0.1 parts of cardamom seed ketone.

[0014] The present invention also provides application of the composition in preparing anti-oxidation and / or anti-wrinkle cosmetics.

[0015] In the present invention, the anti-oxidation comprises increasing the level of Nrf2; further, the Nrf2 is Nrf2 in keratinocytes. Furthermore, the keratinocytes in the present invention are HACAT cells.

[0016] In the present invention, the anti-wrinkle includes: improving fine lines, dry lines and / or improving skin roughness. The wrinkles to be anti-wrinkled are around the face, including but not limited to under-eye lines and / or crow's feet.

[0017] Furthermore, the present invention also provides a cosmetic combination package, which includes the composition as described above.

[0018] In the present invention, the cosmetic combination includes the composition as described above.

[0019] In the cosmetic combination package of the present invention, the mass fraction of PQQ or its salt is 0.0001% - 0.08%;

[0020] The mass fraction of cardamom ketone is 0.001% - 0.8%.

[0021] For example, in cosmetics, the mass fraction of PQQ or its salt is 0.0005%, 0.001% or 0.01%;

[0022] The mass fraction of cardamom ketone is 0.005%, 0.01% or 0.1%.

[0023] In the present invention, the cosmetic combination package is called PQQ PRO, in which PQQ or its salt exists in the form of a solution. The mass fractions of PQQ or its salt and cardamom ketone as described above are both the usage concentrations of the cosmetics. PQQ or its salt and cardamom ketone can exist in 2 - 100 times the usage concentration. For example, it can exist in 2 times, 3 times, 4 times, 5 times, 10 times, 20 times, 30 times, 40 times, 50 times or 100 times the usage concentration. That is, in the cosmetic combination package (or called PQQ PRO), the concentrations of PQQ or its salt and cardamom ketone are 2 - 100 times the usage concentration, and during use, it can be diluted 2 times, 3 times, 4 times, 5 times, 10 times, 20 times, 30 times, 40 times, 50 times or 100 times.

[0024] In the cosmetic combination package of the present invention (or called PQQ PRO), it also includes 0.0001wt% - 99.9999wt% solvent and 0.0001wt% - 10wt% thickener.

[0025] Preferably, the solvent of the present invention is one or more of water, butylene glycol, glycerol, and propylene glycol;

[0026] Preferably, the thickener of the present invention is one or more of acryloyldimethyltauramide / VP copolymer, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, acrylate (ester) / C10 - 30 alkanol acrylate crosslinked polymer, and sodium polyacryloyldimethyltaurate.

[0027] In the cosmetic combination package of the present invention, it can also include at least one active substance. The active substance includes but is not limited to at least one of citrus fruit extract, inositol, hyaluronic acid, vitamin C, vitamin E, niacinamide, retinol, ceramide, arbutin, tranexamic acid, licorice extract, purslane extract, peptide, and collagen.

[0028] Furthermore, the present invention also includes a cosmetic comprising the composition as described above, or comprising the cosmetic combination package as described above.

[0029] The cosmetics provided by the present invention include: the cosmetics combination package as described above and acceptable auxiliary materials in cosmetics; wherein the mass fraction of the cosmetics combination package is 0.1% to 100%.

[0030] In the present invention, the acceptable auxiliary materials in the cosmetics include but are not limited to at least one of a thickener, a pH adjuster, a preservative and water. In the present invention, the thickener is ammonium acryloyldimethyltaurate / VP copolymer, the pH adjuster is sodium hydroxide, and the preservative is phenoxyethanol.

[0031] In a specific embodiment, the mass fraction of the thickener is 0.6%, the mass fraction of the preservative is 0.5%, and the pH adjuster adjusts the pH value of the cosmetic to 5.5-6.5.

[0032] As a feasibility case, the acceptable excipients in the cosmetics include but are not limited to solvents (water, ethanol, propylene glycol), emulsifiers (glyceryl stearate, polysorbate 80), thickeners (carbomer, xanthan gum, hydroxyethyl cellulose), preservatives (sodium benzoate, potassium sorbate, phenoxyethanol), moisturizers (glycerin, hyaluronic acid, urea), antioxidants (vitamin C, vitamin E, tea polyphenols), flavor pigments (flavors, titanium dioxide, iron oxide), pH adjusters (citric acid, triethanolamine), chelating agents (disodium EDTA), propellants (propane, butane), pearlescent agents (ethylene glycol stearate), film formers (polyvinyl alcohol), and skin conditioning agents (allantoin, panthenol).

[0033] As a feasibility case, the dosage forms of the cosmetics include but are not limited to: creams and emulsions, such as ointments, creams, lotions, honey and fats; liquids, such as dew, liquid, water and oil; gels, such as sprays, sprays (without propellants), glues and aerosols (with propellants); powders, such as loose powder, granules and block powders; blocks, such as large solids and block powders; muds, such as muds; wax-based products, such as wax-based products; films, such as patches and films; freeze-dried products, such as freeze-dried powders and freeze-dried tablets.

[0034] The present invention also provides a method for preparing the cosmetic as described above, which comprises: mixing PQQ or its salt, cardamom seed ketone and water.

[0035] A method for skin anti-oxidation and / or wrinkle removal, comprising applying the above composition or the above cosmetics on the skin surface.

[0036] In specific embodiments, the application methods include smearing, wiping, spraying, dotting, patting, massaging, applying, kneading, pressing, rolling, brushing, dipping and smearing, spray painting, dripping, printing, spreading evenly, blending, tapping, thick coating, thin coating, rubbing, kneading and rubbing, sweeping, flicking, spin coating, pushing, pulling, scraping, dabbing, blowing or splashing.

[0037] As a feasibility case, the skin surface includes but is not limited to facial skin, scalp skin, neck skin, shoulder skin, chest skin, abdominal skin, arm skin, hand skin, buttock skin, leg skin, foot skin, armpit skin, groin skin, ear skin, nose skin or lip skin.

[0038] The present invention provides a composition of cardamonin and pyrroloquinoline quinone (PQQ) or its salt. In the present invention, the composition has good stability and shows a significant synergistic effect in terms of antioxidant. In addition, the results of the human anti-wrinkle efficacy questionnaire show that the composition has a better anti-wrinkle effect compared with sodium pyrroloquinoline quinone disodium salt. Detailed implementation mode

[0039] The present invention provides a composition for enhancing the antioxidant efficacy of PQQ, its application, a combination package with cosmetics, and cosmetics. Those skilled in the art can draw on the content of this article and appropriately modify the process parameters to achieve it. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The methods and applications of the present invention have been described through preferred embodiments, and those skilled in the art can obviously make changes or appropriate changes and combinations to the methods and applications in this article without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0040] Unless otherwise defined in the present invention, scientific and technical terms related to the present invention shall have the meanings understood by those of ordinary skill in the art.

[0041] The terms "comprising", "including" and "having" can be used interchangeably, aiming to represent the inclusiveness of the solution, meaning that the solution may contain other elements in addition to the listed elements. At the same time, it should be understood that when using "comprising", "including" and "having" to describe in this article, the solution of "consisting of..." is also provided.

[0042] The term "and / or" describes the association relationship of associated objects, indicating that three relationships can exist. For example, A and / or B can represent: A exists alone, A and B exist simultaneously, and B exists alone. Where A and B can be singular or plural.

[0043] In this application, "at least one" means one or more, and "a plurality" means two or more. "At least one of the following" or similar expressions refer to any combination of these items, including any combination of single item or plural items.

[0044] In this application, the term "combination package" refers to a composite raw material that can be applied to cosmetics, which is pre-mixed from a variety of raw materials in a specific proportion. In this application, it mainly refers to a mixture containing PQQ or its salt, cardamomone and excipients.

[0045] All the test materials used in the present invention are ordinary commercially available products and can be purchased on the market.

[0046] It should be understood that in various embodiments of the present invention, the magnitude of the serial number does not mean the order of execution. Some or all of the steps can be executed in parallel or sequentially. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation to the implementation process of the embodiments of this application.

[0047] The numerical ranges and parameters involved in the present invention have been presented as precisely as possible in the specific embodiments. However, any numerical value inevitably contains standard deviations caused by individual test methods. Therefore, unless otherwise clearly stated, it should be understood that all numerical ranges or specific data used in this disclosure may have certain reasonable deviations within a certain range, for example: within ±10%, ±5%, ±1% or ±0.5%.

[0048] Some cases are described in the examples and comparative examples of the present invention. Among them, the examples show some implementation manners of the present invention. However, this does not mean that the effects of the present invention can only be achieved in these cases. In fact, good effects can be achieved at any concentration of each component between the two endpoint values shown in the examples. Among the cases with relatively good effects, the mass ratio of PQQ-2Na to cardamomone is 1:10. Some cases with poor effects in the tests are also listed in the following examples, such as compounding with different substances and PQQ-2Na or adopting different ratios, etc. However, the effects of these attempts are not as good as those under the ratio described above, and will not be elaborated here.

[0049] All the test materials used in the present invention are ordinary commercially available products and can be purchased on the market. The cardamomone is purchased from Symrise, and the active ingredient is hydroxymethoxyphenyldecanone. The present invention will be further described below in conjunction with the examples:

[0050] Examples

[0051] Prepare samples according to the following table formula. The formula tables of the examples and comparative examples are shown in Table 1 below, and the proportions between the components in Table 1 are by mass.

[0052] Table 1 Formula table of embodiments and comparative examples (mass ratio or mass fraction %)

[0053]

[0054] The preparation method comprises: weighing PQQ-2Na and cardamom ketone or glucosylrutin or sodium pyrosulfite or tris(tetramethylhydroxypiperidinol) citrate according to the mass fraction percentage in Table 1, dissolving them in water and stirring until they are completely dissolved.

[0055] 2. Stability test

[0056] Examples 1-2 and Comparative Examples 1, 7-8, and 10-12 were stored at 25°C, 4°C, 40°C, and 48°C for 4 weeks, and the color and odor of each sample at the initial state, 2 weeks, and 4 weeks were observed. The results are shown in Table 2:

[0057] 2.1 Stability test results

[0058] Table 2 Sample stability test

[0059]

[0060] Stability results: Examples 1-2, Comparative Example 1, and Comparative Examples 7-8 passed the 4-week high and low temperature stability; Comparative Examples 10-12 had abnormal appearance after 4 weeks of stability and failed the stability test.

[0061] 2.2 Efficacy test

[0062] 2.2.1 NRF2 antioxidant test

[0063] Purpose of the test: This test uses human immortalized keratinocytes as the research object, and determines Nrf2 in the cells by immunofluorescence, in order to provide an experimental basis for the test of the antioxidant efficacy of the test object.

[0064] Test principle: The Nrf2 / ARE (nuclear factor E2-related factor 2 / antioxidant response element) pathway plays a crucial role in the antioxidant and reduction processes of the body, and exerts extensive cytoprotective functions in aspects such as anti-tumor, anti-apoptosis, anti-inflammatory response, anti-atherosclerosis, and neuroprotection. Nrf2 is a transcription factor with a basic leucine zipper structure, widely present in various organs of the body, and is the main regulatory factor for cellular redox reactions. Under normal physiological conditions, Nrf2 mainly binds to Keap1 (Kelch ECH-associated protein 1), exists in the cytoplasm in its inactive state, and is rapidly degraded through the ubiquitin-proteasome pathway to maintain the low transcriptional activity of Nrf2. When stimulated, Nrf2 dissociates from Keap1, and the activated Nrf2 translocates into the nucleus, binds to the Maf protein to form a heterodimer, and then binds to ARE, activating the expression of target genes and regulating the transcriptional activities of phase II metabolic enzymes, antioxidant enzymes, or drug transporters, thereby exerting an antioxidant damage effect.

[0065] The immunofluorescence technique is based on the principle of antigen-antibody reaction. First, the known nuclear factor E2-related factor 2 (NRF2) antigen or antibody is labeled with a fluorescent group, and then this fluorescent antibody (or antigen) is used as a probe to bind to the corresponding FLG antigen (or antibody) in cells or tissues. Using a fluorescence microscope, the cells or tissues where the fluorescence is located can be seen, thereby determining the nature, location, and up-regulation or down-regulation of the expression level of the antigen or antibody. Then, the ImageJ software is used to convert the picture results into visualized data.

[0066] Table 3 Main reagents

[0067]

[0068] Table 4 Main equipment

[0069]

[0070] The test methods include:

[0071] 1) Cell seeding: Seed 20,000 HACAT cells per well in a 96-well plate and culture in an incubator at 37°C and 5% CO2 for 24 h.

[0072] 2) Solution preparation: The blank group is not added with samples and UV for modeling, the negative group is added with UV for modeling, and the example and comparative example groups are added with samples and UV for modeling.

[0073] 3) Drug administration: When the cell confluence rate in the 96-well plate reaches 40% - 60%, perform grouped drug administration. After drug administration, place the 96-well plate in an incubator (37°C, 5% CO2) and culture for 24 h.

[0074] 4) Determination of intracellular NRF2 content:

[0075] Sample collection: Aspirate and discard the culture medium, and wash the cells 3 times with PBS (300 μl / well);

[0076] Cell fixation: Aspirate and discard the PBS, add 4% paraformaldehyde fixative (100 μl / well) to fix the cells, and let stand at room temperature for 20 min;

[0077] Washing: Wash 5 times with PBS (300 μl / well) to remove residual paraformaldehyde;

[0078] Washing: Wash 5 times with PBS (300 μl / well) to remove residual 0.1% Triton-100;

[0079] Blocking: Use 5% FBS blocking solution prepared with PBS, 100 μl / well, and incubate at room temperature for 1 hour;

[0080] Incubate with primary antibody: Under light protection, dilute the primary antibody of NRF2 at a ratio of 1:200 with immunofluorescence diluent. Do not wash the well plate after removing the liquid, add 50 μl / ml of the primary antibody diluent, and incubate overnight at 4 °C in the dark;

[0081] Washing: Re-warm for 30 min, aspirate and discard the primary antibody under light protection, and wash the cells 3 times with the washing solution (300 μl / well), 5 min each time;

[0082] Incubate with fluorescent secondary antibody: Under light protection, dilute the fluorescent secondary antibody at a ratio of 1:200 with fluorescent diluent, and incubate at room temperature for 1 hour;

[0083] 5) Image collection: After washing three times (300 μl / well), leave 50 μl of PBS in each well, and observe and take pictures under an inverted fluorescence microscope.

[0084] 6) Calculate the average fluorescence intensity value of the image fluorescence intensity through ImageJ software.

[0085] Result statistical analysis:

[0086] Use GraphPad Prism 8.0 software for data statistical analysis and draw charts. Measurement data are expressed as x±s. One-way ANOVA is used for inter-group differences. A P value < 0.05 indicates a statistically significant difference, P < 0.01 indicates a highly significant difference, and P < 0.001 indicates an extremely significant difference.

[0087] Table 5 Results of NRF2 antioxidant test:

[0088]

[0089] The increase rate relative to the negative control in the table = (average fluorescence intensity value of the sample group - average fluorescence intensity value of the negative control group) / average fluorescence intensity value of the negative control group.

[0090] The Bliss independent model is introduced to calculate the synergistic effect of the data results:

[0091] Taking the incidence rate of the drug target as the efficacy index, the magnitude range of the efficacy is 0 - 1. Taking drugs A and B as examples, regarding the drug effects of drugs A and B as independent events, borrowing the probability formula P AB =P A +P B -P A *P B , and using this formula as the calculation formula E for the expected additive effect of combined drug application AB =E A +E B -E A *E B . Taking the ratio of the actual efficacy value to the value calculated by this formula as CI, the calculation formula is as shown in formula (V).

[0092]

[0093] CI > 1 indicates synergy, CI = 1 indicates addition, and CI < 1 indicates antagonism.

[0094] The Nrf2 experiment results show that the Nrf2 content in the blank group is 151.988, and the Nrf2 content in the negative group after UV treatment is 127.75, showing a significant decrease compared with the blank group. The NRF2 contents in the examples and comparative examples are all higher than that in the negative group.

[0095] ① According to the results of Example 1, Comparative Example 1 and Comparative Example 5, it can be seen that Comparative Example 5 has no effect, and the effect of Example 1 is better than that of Comparative Example 1. The calculation of the synergistic effect of Example 1, Comparative Example 1 and its Comparative Example 5:

[0096] CI = 0.260399 / (0.19384 + 0.052266 - 0.19384 * 0.052266) = 1.103506

[0097] CI > 1, indicating that cardamom ketone:pqq = 10:1 has the effect of enhancing the antioxidant property of pqq.

[0098] ② Repeat the ratio of cardamom ketone to PQQ = 10:1, i.e., Example 2, Comparative Example 2, and Comparative Example 4. It can be seen that Comparative Example 4 (single cardamom ketone) has no effect, and the effect of Example 2 after combining Comparative Example 2 and 4 is better than that of Comparative Example 2 (single PQQ). Calculate the synergistic effect of Example 2, Comparative Example 2, and Comparative Example 4:

[0099] CI = 0.296916 / (0.21207 + 0.066935 - 0.21207 * 0.066935) = 1.121238

[0100] CI > 1 indicates that the ratio of cardamom ketone to PQQ = 10:1 has the effect of enhancing the antioxidant property of PQQ at two different addition amounts;

[0101] ③ According to the data of Comparative Example 7, Comparative Example 1, and Comparative Example 3, calculate CI = 0.835438, CI < 1, indicating that the ratio of cardamom ketone to PQQ = 1:1 has no effect of enhancing the antioxidant property of PQQ;

[0102] ④ According to the data of Comparative Example 8, Comparative Example 1, and Comparative Example 6, calculate CI = 0.668009, CI < 1, indicating that the ratio of cardamom ketone to PQQ = 50:1 has no effect of enhancing the antioxidant property of PQQ.

[0103] In summary, the ratio of cardamom ketone to PQQ = 10:1 is the optimal ratio.

[0104] 2.2.2 Human efficacy test (anti-wrinkle questionnaire)

[0105] 2.2.2.1 Test principle

[0106] The anti-aging efficacy of different samples was preliminarily evaluated by subjective evaluation, and the anti-aging performance of the optimized formula was used as the evaluation standard.

[0107] 2.2.2.2 Test method

[0108] Recruit more than 30 people to conduct a consumer questionnaire survey on the left and right half faces using Example 3 and Comparative Example 9 to verify the anti-wrinkle efficacy comparison between Example 3 and Comparative Example 9 after 28 days of use. Use 3 pieces per week.

[0109] 2.2.2.3 Self-evaluation of the subjects

[0110] Table 6

[0111]

[0112] 2.2.2.4 Test environment

[0113] Room temperature environment.

[0114] 2.2.2.5 Test Process

[0115] Experimental Design

[0116] After using the test sample product for 28 days, a consumer self-evaluation test is conducted.

[0117] After using the sample for 28 days

[0118] The subjects fill in the consumer questionnaire. After completing the above evaluations, this test ends.

[0119] 2.2.2.6 Test Results

[0120] Table 7 Consumer Evaluation of Using the Test Sample (28 days)

[0121]

[0122] Note: The evaluation index (1 point means extremely dissatisfied, 2 points means dissatisfied, 3 points means average, 4 points means relatively satisfied, 5 points means satisfied), and the satisfaction rate refers to the proportion of the number of people with N>4 points.

[0123] The test results show that the anti-wrinkle effect of the composition of Example 3 is better than that of Comparative Example 9 using PQQ alone.

[0124] The above are only the preferred embodiments of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A composition for enhancing the antioxidant efficacy of PQQ, characterized in that, The invention is composed of PQQ or its salt and cardamom ketone; the mass ratio of the PQQ or its salt to cardamom ketone is 1:

10.

2. The composition according to claim 1, characterized in that It is composed of 0.0005 parts of PQQ-2Na and 0.005 parts of cardamom seed ketone; or consisting of 0.001 parts of PQQ-2Na and 0.01 parts of cardamom seed ketone; Or it is composed of 0.01 parts of PQQ-2Na and 0.1 parts of cardamom seed ketone.

3. Use of the composition according to claim 1 or 2 in the preparation of antioxidant and / or anti-wrinkle cosmetics.

4. The use according to claim 3, characterized in that: The antioxidant effect includes increasing the level of Nrf2; The anti-wrinkle effect includes: improving fine lines, dry lines and / or improving skin roughness.

5. The use according to claim 4, characterized in that: The Nrf2 is Nrf2 in keratinocytes; The wrinkles to be treated include under-eye wrinkles and / or crow's feet.

6. A combination package of cosmetics, characterized in that, The invention comprises the composition according to claim 1 or 2.

7. The combination package according to claim 6, characterized in that, in: The mass fraction of PQQ or its salt is 0.0001%~0.08%; The mass fraction of cardamom ketone is 0.001%~0.8%.

8. The combination package according to claim 6, characterized in that, Also includes 0.0001wt%~99.9999wt% solvent and / or 0.0001wt%~10wt% thickener; The solvent is one or more of water, butylene glycol, glycerol, and propylene glycol; The thickener is one or more of ammonium acryloyldimethyltaurate / VP copolymer, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, and sodium polyacryloyldimethyltaurate.

9. The combination package according to any one of claims 6 to 8, characterized in that, The invention also includes at least one active substance, which is selected from one or more of citrus fruit extract and inositol.

10. A cosmetic, characterized in that, The invention comprises the composition according to claim 1 or 2, or the combination package according to any one of claims 6 to 9.

11. The cosmetic according to claim 10, characterized in that, The cosmetics also include auxiliary materials; the mass fraction of the combination package is 0.1% to 100%.

12. The cosmetic according to claim 11, characterized in that, The acceptable auxiliary materials in the cosmetics are at least one of a thickener, a pH adjuster, a preservative and water; The thickener is ammonium acryloyldimethyltaurate / VP copolymer; The pH adjuster is sodium hydroxide; The preservative is phenoxyethanol.

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