Hair-fixing and hair-loss-preventing composition, application thereof and hair-fixing and hair-loss-preventing product

Abstract

The invention discloses a hair-fixing and hair-loss-preventing composition, application thereof and a hair-fixing and hair-loss-preventing product. The hair fixing and hair loss preventing composition comprises 17-type collagen and a plant extract, wherein the plant extract comprises a cacumen biotae extract. On the basis of inhibiting 5alpha-reductase and resisting oxidation, the composition disclosed by the invention also has the effects of fixing hair and preventing hair loss by virtue of ways of relieving inflammatory response, activating hair papilla cells, maintaining health of the hair papilla cells, reducing hair follicle senescence cells and the like.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and particularly to a hair-fixing and anti-hair loss composition, its application, and a hair-fixing and anti-hair loss product. Background Art

[0002] The problem of hair loss is multi-dimensional, and its impact is not limited to the physical appearance level, but more widely involves multiple aspects such as mental health, social activities, economic status, and medical health. From a psychological perspective, hair loss can significantly reduce an individual's self-esteem and self-image perception, thereby inducing negative psychological states such as anxiety and depression, having an adverse impact on social activities, and in severe cases, may even lead to social isolation. At the same time, gender identity and personal attractiveness may also be affected by hair loss. In the field of sociology, the negative impact of hair loss on career development cannot be ignored, especially in those professional fields with strict appearance requirements, where the impact is more significant. In addition, hair loss may also interfere with the evaluation of self-confidence and attractiveness in interpersonal relationships, reducing the overall quality of life. Economically, the financial burden brought by hair loss treatment cannot be ignored, including the high costs of hair transplantation and special hair care products.

[0003] Hair loss is a complex physiological phenomenon affected by multiple factors. There are many reasons for hair loss. External environments such as ultraviolet rays, chemical substances, and heat treatment can all damage the hair and exacerbate hair loss. Research shows that nutritional status is also crucial for hair health. Lack of nutrients such as protein, iron, zinc, and vitamins will directly affect hair growth and quality. At the same time, certain systemic diseases, such as thyroid diseases and autoimmune diseases, are also closely related to hair loss. Drug use is also a major inducement of hair loss. Many drugs such as chemotherapy drugs and antidepressants may cause hair loss. Scalp health problems cannot be underestimated either. Microbiological imbalance, fungal infections, and inflammatory diseases can all lead to hair loss. Psychological factors such as stress and anxiety indirectly affect hair growth by influencing hormone levels and the immune system. Physical damage, poor hair care habits, and physiological processes such as pregnancy and childbirth may also cause hair loss. Finally, as people age, the hair growth cycle changes, which is also an important reason for hair loss.

[0004] Genetic factors are also crucial among the factors affecting hair loss. Especially in androgenetic alopecia, genetic susceptibility plays a decisive role. Changes in hormone levels cannot be ignored either. For example, an increase in androgens can trigger follicular atrophy, thereby leading to hair loss. Hair follicle stem cells play a key role in the hair growth cycle. The hair growth cycle includes the anagen, catagen, and telogen phases. Among them, hair follicle stem cells are activated during the anagen phase to promote new hair growth. The decline or disorder of the function of hair follicle stem cells can cause hair loss because they cannot maintain the anagen phase of the hair follicle, resulting in sparse hair and hair loss. Especially in androgenetic alopecia, androgens may interfere with hair follicle stem cells, triggering follicular atrophy and hair thinning.

[0005] There are also a large number of anti - hair - loss and hair - strengthening compositions and formulas on the market currently. Chinese Patent Application CN118845516A discloses an anti - hair - loss composition, which is composed of adenosine, coffee extract, L - carnitine tartrate, tripeptide - 1 copper, Platycladus orientalis extract and nicotinamide. This composition has significant synergistic effects in improving cell antioxidant capacity and inhibiting 5α - reductase. However, this composition has no obvious effect on inhibiting the senescence or disorder of hair follicle stem cells and maintaining the anagen phase of hair follicles. Summary of the Invention

[0006] To solve the above problems, the present invention provides a hair - strengthening and anti - hair - loss composition, its application, and a hair - strengthening and anti - hair - loss product. On the basis of inhibiting 5α - reductase and antioxidant, the composition of the present invention also exerts the effects of hair strengthening and anti - hair - loss through ways such as reducing inflammatory response, activating dermal papilla cells, maintaining the health of dermal papilla cells, and reducing senescent cells in hair follicles.

[0007] To achieve the above - mentioned technical objectives, the present invention provides the following technical solutions.

[0008] In a first aspect, the present invention provides a hair - strengthening and anti - hair - loss composition, which includes type XVII collagen and a plant extract, and the plant extract includes Platycladus orientalis extract.

[0009] In the present invention, the type XVII collagen can be obtained by conventional chemical synthesis methods or genetic engineering methods in the art.

[0010] In the present invention, the Platycladus orientalis extract can be extracted from the branch tips or branches and leaves of the plant Platycladus orientalis (L.) Franco of the family Cupressaceae. The extraction method can be conventional in the art.

[0011] In some embodiments, the hair - strengthening and anti - hair - loss composition consists of type XVII collagen and a plant extract.

[0012] In some preferred embodiments, the hair - strengthening and anti - hair - loss composition consists of type XVII collagen and Platycladus orientalis extract.

[0013] In some embodiments, the molecular weight of the type XVII collagen is 20 - 200 kDa, such as 23.8 kDa, 97 kDa, 120 kDa or 180 kDa.

[0014] In some embodiments, the mass ratio of the type XVII collagen to the Platycladus orientalis extract is (0.0001 - 0.1):(0.1 - 5), such as 1:100.

[0015] In some embodiments, the hair-fixing and anti-hair loss composition further comprises a plant extract other than the Platycladus orientalis extract, and the plant extract is preferably selected from one or more of Magnolia officinalis extract, Polygonum multiflorum extract, Zingiber officinale extract, Salvia miltiorrhiza extract, and Coffea arabica extract.

[0016] In the present invention, the Magnolia officinalis extract can be obtained by extracting from the dried root bark, stem bark or branch bark of Magnolia officinalis of the genus Magnolia in the Magnoliaceae family. The extraction method can be conventional in the art.

[0017] In the present invention, the Polygonum multiflorum extract can be obtained by extracting from the dried tuberous roots of Polygonum multiflorum of the Polygonaceae family. The extraction method can be conventional in the art.

[0018] In the present invention, the Salvia miltiorrhiza extract can be obtained by extracting from the dried roots and rhizomes of Salvia miltiorrhiza of the Lamiaceae family. The extraction method can be conventional in the art.

[0019] In the present invention, the Coffea arabica extract can be obtained by extracting from the coffee fruit or coffee seeds of plants of the genus Coffea in the Rubiaceae family. The extraction method can be conventional in the art.

[0020] In some embodiments, the hair-fixing and anti-hair loss composition comprises type XVII collagen, Platycladus orientalis extract, and Magnolia officinalis extract.

[0021] In some embodiments, the hair-fixing and anti-hair loss composition consists of type XVII collagen, Platycladus orientalis extract, and Magnolia officinalis extract.

[0022] In the above embodiments, preferably, the mass ratio of type XVII collagen, Platycladus orientalis extract, and Magnolia officinalis extract is (0.0001 - 0.1):(0.1 - 5):(0.1 - 2), for example, 1:100:100.

[0023] In some embodiments, the hair-fixing and anti-hair loss composition comprises type XVII collagen, Platycladus orientalis extract, and Polygonum multiflorum extract.

[0024] In some embodiments, the hair-fixing and anti-hair loss composition consists of type XVII collagen, Platycladus orientalis extract, and Polygonum multiflorum extract.

[0025] In the above embodiments, preferably, the mass ratio of type XVII collagen, Platycladus orientalis extract, and Polygonum multiflorum extract is (0.0001 - 0.1):(0.1 - 5):(0.1 - 10), for example, 1:100:100.

[0026] In some embodiments, the hair-fixing and anti-hair loss composition comprises type XVII collagen, Platycladus orientalis extract, and Zingiber officinale extract.

[0027] In some embodiments, the hair-fixing and anti-hair-loss composition consists of type XVII collagen, Platycladus orientalis leaf extract, and ginger extract.

[0028] In the above embodiments, preferably, the mass ratio of the type XVII collagen, Platycladus orientalis leaf extract, and ginger extract is (0.0001 - 0.1):(0.1 - 5):(0.1 - 10), for example, 1:100:100.

[0029] In some embodiments, the hair-fixing and anti-hair-loss composition includes type XVII collagen, Platycladus orientalis leaf extract, and Salvia miltiorrhiza extract.

[0030] In some embodiments, the hair-fixing and anti-hair-loss composition consists of type XVII collagen, Platycladus orientalis leaf extract, and Salvia miltiorrhiza extract.

[0031] In the above embodiments, preferably, the mass ratio of the type XVII collagen, Platycladus orientalis leaf extract, and Salvia miltiorrhiza extract is (0.0001 - 0.1):(0.1 - 5):(0.1 - 6), for example, 1:100:100.

[0032] In some embodiments, the hair-fixing and anti-hair-loss composition includes type XVII collagen, Platycladus orientalis leaf extract, and coffee extract.

[0033] In some embodiments, the hair-fixing and anti-hair-loss composition consists of type XVII collagen, Platycladus orientalis leaf extract, and coffee extract.

[0034] In the above embodiments, preferably, the mass ratio of the type XVII collagen, Platycladus orientalis leaf extract, and coffee extract is (0.0001 - 0.1):(0.1 - 5):(0.0025 - 5), for example, 1:100:2.5.

[0035] In some embodiments, the hair-fixing and anti-hair-loss composition further includes niacinamide.

[0036] In some embodiments, the hair-fixing and anti-hair-loss composition consists of type XVII collagen, Platycladus orientalis leaf extract, and niacinamide.

[0037] In the above embodiments, preferably, the mass ratio of the type XVII collagen, Platycladus orientalis leaf extract, and niacinamide is (0.0001 - 0.1):(0.1 - 5):(0.05 - 5), for example, 1:100:500.

[0038] In a second aspect, the present invention provides a hair-fixing and anti-hair-loss product, which includes the hair-fixing and anti-hair-loss composition as described above.

[0039] In some embodiments, the hair strengthening and anti - hair loss product is shampoo, hair conditioner, hair essence, body wash or body lotion.

[0040] In a third aspect, the present invention provides the use of the hair strengthening and anti - hair loss composition as described above in the preparation of a hair strengthening and anti - hair loss product.

[0041] On the basis of conforming to the common knowledge in the art, the above - mentioned preferred conditions can be combined arbitrarily to obtain the preferred examples of the present invention.

[0042] The reagents and raw materials used in the present invention are all commercially available.

[0043] The positive and progressive effects of the present invention are as follows:

[0044] 1. The present invention combines type XVII collagen and Platycladus orientalis leaf extract. On the basis of inhibiting 5α - reductase and antioxidant effects, it also exerts hair strengthening and anti - hair loss effects by activating dermal papilla cells, maintaining the health of dermal papilla cells and reducing follicular senescent cells. In addition, the composition of the present invention also has the effect of reducing inflammatory responses.

[0045] 2. The preferred composition of the present invention combines with other traditional Chinese medicine materials, giving full play to the characteristics of comprehensive regulation, mildness, safety, overall regulation, equal emphasis on treating the symptoms and root causes, and multi - target treatment of traditional Chinese medicine, and exerting the effects of anti - hair loss, hair strengthening and hair nourishing from multiple perspectives.

[0046] 3. The composition of the present invention is easy to obtain materials, safe, healthy and has stable efficacy, and is suitable for large - scale industrial production. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 It is a test result graph of the 5α - reductase inhibition rate test for each component and the composition.

[0048] Figure 2 It is a test result graph of the antioxidant capacity test for each component and the composition.

[0049] Figure 3 It is a test result graph of the ability to promote COL17 expression for each component and the composition.

[0050] Figure 4 It is a test result graph of the ability to promote TGF - β2 expression for each component and the composition.

[0051] Figure 5 It is a test result graph of the ability to promote VEGFA expression for each component and the composition.

[0052] Figure 6 It is a test result graph of the ability to reduce follicular senescent cells for each component and the composition. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0053] The present invention will be further described below by way of examples, but the present invention is not limited to the scope of the described examples. For the experimental methods without specific conditions in the following examples, they are carried out according to conventional methods and conditions, or selected according to the product instructions.

[0054] Raw materials such as type XVII collagen, Platycladus orientalis extract, Magnolia officinalis extract, Polygonum multiflorum extract, Zingiber officinale extract, Salvia miltiorrhiza extract, Coffea arabica extract, Acorus calamus extract, and niacinamide were all purchased from raw material suppliers.

[0055] The information of the manufacturers and models of each raw material component and instrument is as follows:

[0056]

[0057]

[0058] Preparation of anti - hair - loss and hair - strengthening composition 1 in Example 1

[0059] Mix type XVII collagen and Platycladus orientalis extract in a mass ratio of 1:100 to obtain anti - hair - loss and hair - strengthening composition 1.

[0060] For subsequent tests, anti - hair - loss and hair - strengthening composition 1 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen and Platycladus orientalis extract in the solution were 0.001% and 0.1% respectively.

[0061] Preparation of anti - hair - loss and hair - strengthening composition 2 in Example 2

[0062] Mix type XVII collagen, Platycladus orientalis extract and Magnolia officinalis extract in a mass ratio of 1:100:100 to obtain anti - hair - loss and hair - strengthening composition 2.

[0063] For subsequent tests, anti - hair - loss and hair - strengthening composition 2 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, Platycladus orientalis extract and Magnolia officinalis extract in the solution were 0.001%, 0.1% and 0.1% respectively.

[0064] Preparation of anti - hair - loss and hair - strengthening composition 3 in Example 3

[0065] Mix type XVII collagen, Platycladus orientalis extract and Polygonum multiflorum extract in a mass ratio of 1:100:100 to obtain anti - hair - loss and hair - strengthening composition 3.

[0066] For subsequent tests, anti - hair - loss and hair - strengthening composition 3 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, Platycladus orientalis extract and Polygonum multiflorum extract in the solution were 0.001%, 0.1% and 0.1% respectively.

[0067] Preparation of anti - hair - loss and hair - strengthening composition 4 in Example 4

[0068] Mix type XVII collagen, Platycladus orientalis leaf extract, and ginger extract in a mass ratio of 1:100:100 to obtain the hair-fixing and anti-hair-loss composition 4.

[0069] For subsequent tests, dissolve the hair-fixing and anti-hair-loss composition 4 in water to prepare an aqueous solution, where the contents of type XVII collagen, Platycladus orientalis leaf extract, and ginger extract in the solution are 0.001%, 0.1%, and 0.1% respectively.

[0070] Example 5 Preparation of the hair-fixing and anti-hair-loss composition 5

[0071] Mix type XVII collagen, Platycladus orientalis leaf extract, and Salvia miltiorrhiza extract in a mass ratio of 1:100:100 to obtain the hair-fixing and anti-hair-loss composition 5.

[0072] For subsequent tests, dissolve the hair-fixing and anti-hair-loss composition 5 in water to prepare an aqueous solution, where the contents of type XVII collagen, Platycladus orientalis leaf extract, and Salvia miltiorrhiza extract in the solution are 0.001%, 0.1%, and 0.1% respectively.

[0073] Example 6 Preparation of the hair-fixing and anti-hair-loss composition 6

[0074] Mix type XVII collagen, Platycladus orientalis leaf extract, and coffee extract in a mass ratio of 2:200:5 to obtain the hair-fixing and anti-hair-loss composition 6.

[0075] For subsequent tests, dissolve the hair-fixing and anti-hair-loss composition 6 in water to prepare an aqueous solution, where the contents of type XVII collagen, Platycladus orientalis leaf extract, and coffee extract in the solution are 0.001%, 0.1%, and 0.0025% respectively.

[0076] Example 7 Preparation of the hair-fixing and anti-hair-loss composition 7

[0077] Mix type XVII collagen, Platycladus orientalis leaf extract, and niacinamide in a mass ratio of 1:100:500 to obtain the hair-fixing and anti-hair-loss composition 7.

[0078] For subsequent tests, dissolve the hair-fixing and anti-hair-loss composition 7 in water to prepare an aqueous solution. The contents of type XVII collagen, Platycladus orientalis leaf extract, and niacinamide in the solution are 0.001%, 0.1%, and 0.5% respectively.

[0079] Comparative Example 1 Preparation of the comparative composition 1

[0080] Mix type XVII collagen and Magnolia officinalis extract in a mass ratio of 1:100 to obtain the comparative composition 1.

[0081] For subsequent tests, Comparative Composition 1 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen and Magnolia officinalis extract in the solution were 0.001% and 0.1% respectively.

[0082] Comparative Example 2 Preparation of Comparative Composition 2

[0083] Type XVII collagen and Polygonum multiflorum extract were mixed in a mass ratio of 1:100 to obtain Comparative Composition 2.

[0084] For subsequent tests, Comparative Composition 2 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen and Polygonum multiflorum extract in the solution were 0.001% and 0.1% respectively.

[0085] Comparative Example 3 Preparation of Comparative Composition 3

[0086] Type XVII collagen and Zingiber officinale extract were mixed in a mass ratio of 1:100 to obtain Comparative Composition 3.

[0087] For subsequent tests, Comparative Composition 3 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen and Zingiber officinale extract in the solution were 0.001% and 0.1% respectively.

[0088] Comparative Example 4 Preparation of Comparative Composition 4

[0089] Type XVII collagen and Coffea arabica extract were mixed in a mass ratio of 1:2.5 to obtain Comparative Composition 4.

[0090] For subsequent tests, Comparative Composition 4 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen and Coffea arabica extract in the solution were 0.001% and 0.0025% respectively.

[0091] Comparative Example 5 Preparation of Comparative Composition 5

[0092] Type XVII collagen and Acorus calamus extract were mixed in a mass ratio of 1:100 to obtain Comparative Composition 5.

[0093] For subsequent tests, Comparative Composition 5 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen and Acorus calamus extract in the solution were 0.001% and 0.1% respectively.

[0094] Effect Example 1: 5α-Reductase Inhibition Rate Test

[0095] 1. Test Procedure

[0096] (1) Sample tube: Add 1 mL of sample solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, gently shake well, pipette 200 μL into a 96-well microplate, make three parallels for each sample, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measured value A 样品0 . After incubating the sample at 37 °C for 20 minutes, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the second measured value A 样品20 .

[0097] (2) Enzyme tube: Add 1 mL of PBS solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, gently shake well, pipette 200 μL into a 96-well microplate, make three parallels for each sample, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measured value A 酶0 . After incubating the sample at 37 °C for 20 minutes, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the second measured value A 酶20 ; At the same time, perform blank and positive control detections.

[0098] (3) The 5α-reductase inhibition rate is calculated according to the following formula:

[0099]

[0100] Each sample is tested three times, and the average value is taken and recorded in Table 1.

[0101] 2. Test results

[0102] Table 1 Test results of 5α-reductase inhibition rate

[0103]

[0104]

[0105] The experimental results are shown in Table 1 and Figure 1 as follows. Compared with components 1-8, Examples 1-7 have a significant improvement in inhibiting 5α-reductase activity; compared with Example 1, Examples 2-7 have a stronger ability to inhibit 5α-reductase activity. Compared with Comparative Examples 1-5, Example 1 has a stronger ability to inhibit 5α-reductase activity. It can be seen that 17-type collagen and Platycladus orientalis extract have a better cooperative effect compared with other plant extracts.

[0106] Effect Example 2: Antioxidant ability test

[0107] 1. Test procedure

[0108] (1) Dermal papilla cell seeding: According to 3×10 5Seed cells at a seeding density of cells per well into a 6-well plate, and place the 6-well plate in an incubator (37 °C, 5% CO2) and incubate overnight.

[0109] (2) Prepare the solutions: Prepare test substances at different concentrations according to different test groups.

[0110] (3) H2O2 stimulation: According to the test groups, when the cell confluence rate in the 6-well plate reaches about 60%, perform H2O2 stimulation for 1 hour.

[0111] (4) Administration: After the induction time ends, administer according to the test groups, add 2 mL of sample to each well, and set 3 replicate wells for each group. Incubate in a 37 °C, 5% CO2 incubator for 23 hours.

[0112] (5) Reactive oxygen species (ROS) detection: After incubation, add 1 mL of 10 μM DCFH-DA probe to each well, and incubate in an incubator (37 °C, 5% CO2) for 30 minutes; discard the culture medium containing DCFH-DA, wash 3 times with PBS, digest the cells with trypsin (0.25%), wash the cells 1 time with PBS, add a certain amount of fresh PBS, and perform flow cytometry detection.

[0113] (6) Calculate the ROS inhibition rate according to the following formula:

[0114]

[0115] For each sample, take the average of the test results of 3 replicate wells and record it in Table 2.

[0116] 2. Test results

[0117] Table 2 Test results of antioxidant capacity

[0118]

[0119] The experimental results are shown in Table 2 and Figure 2 as follows. After treatment with components 1-8 at the above concentrations, the content of reactive oxygen species (ROS) decreased slightly. After treatment with Examples 1-7 at the above concentrations, the content of reactive oxygen species (ROS) decreased significantly. It can be seen that the composition has a stronger ability to inhibit the production of ROS compared to single raw material components. Compared with Comparative Examples 1-5, Example 1 has a stronger ability to inhibit ROS. It can be seen that 17-type collagen and Platycladus orientalis extract have a better cooperative effect compared to other plant extracts.

[0120] Effect Example 3: Test on the ability to promote the expression of COL17, TGF-β2 and VEGFA

[0121] 1. Test procedure

[0122] (1) Seed dermal papilla cells at a density of 1.5×10 5 cells per well in a 6-well plate and incubate overnight in a CO2 incubator (37°C, 5% CO2).

[0123] (2) Administration of drugs and stimulation. Administer drugs and apply stimulation according to the test groups. Drug administration and stimulation are carried out simultaneously, with 3 replicate wells set for each group. In the BC (blank control) group, add 2 mL of culture medium to each well; in the NC (negative control) group, add 2 mL of culture medium containing H2O2 to each well; in the PC (positive control) group, add 2 mL of culture medium containing H2O2 and WY14643 to each well; in the sample group, add culture medium containing H2O2 and the test sample at the corresponding concentration to each well. After completion, transfer all 6-well plates to a CO2 incubator (37°C, 5% CO2) and incubate for 24 hours.

[0124] (3) After incubation, aspirate the old medium, wash twice with 1 mL / well of PBS, add 1 mL of AG RNAex ProReagent to each well, pipette to lyse the cells, and then collect the samples. Extract RNA, reverse transcribe it into cDNA, and then perform fluorescence quantitative PCR detection. Use the 2 -△△CT method for calculation.

[0125] (4) Calculation of upregulation rate:

[0126]

[0127] For each sample, take the average of the test results of 3 replicate wells and record it in Tables 3 - 5.

[0128] 2. Test results

[0129] Table 3 COL17 expression results

[0130]

[0131]

[0132] The experimental results are shown in Table 3 and Figure 3 As shown, after treatment with the above concentrations, the upregulation rate of the COL 17 gene expression for Component 1 (Collagen type XVII) was 44.23%; for Component 2 (Extract of Platycladus orientalis), the upregulation rate of the COL 17 gene expression was 17.31%; for Components 3 and 5 - 8, after treatment with the above concentrations, the COL 17 gene expression increased slightly, but the upregulation rate did not exceed 10%; for Component 4, after treatment with the above concentrations respectively, the COL 17 gene expression did not increase.

[0133] After the above-mentioned concentrations were used for treatment in Examples 1-7, the expression level of the COL 17 gene increased significantly, and the up-regulation rate ranged from 63.46% to 91.67%. It can be seen that the composition has a stronger ability to promote the expression of the COL 17 gene compared with the single raw material components. Compared with Comparative Examples 1-5, Example 1 has a greater up-regulation rate of the COL 17 gene expression level. It can be seen that type XVII collagen and Platycladus orientalis extract have a better cooperative effect compared with other plant extracts.

[0134] Table 4 TGF-β2 expression results

[0135]

[0136]

[0137] As shown in Table 4 and Figure 4 as shown, after treatment with Component 1 (type XVII collagen) at the above concentration, the up-regulation rate of the TGF-β2 gene expression level was 144.81%, and after treatment with Component 2 (Platycladus orientalis extract) at the above concentration, the up-regulation rate of the TGF-β2 gene expression level was 38.31%; after treatment with Components 3-6 and 8 at the above concentration, the TGF-β2 gene expression level increased slightly, but the up-regulation rate did not exceed 20%; after treatment with Component 7 at the above concentration, the TGF-β2 gene expression level did not increase, but decreased slightly.

[0138] After the above-mentioned concentrations were used for treatment in Examples 1-7, the expression level of the TGF-β2 gene increased significantly, and the up-regulation rate ranged from 45.45% to 324.68%. It can be seen that the composition has a stronger ability to promote the expression of the TGF-β2 gene compared with the single raw material components. Compared with Comparative Examples 1-5, Example 1 has a greater up-regulation rate of the TGF-β2 gene expression level. It can be seen that type XVII collagen and Platycladus orientalis extract have a better cooperative effect compared with other plant extracts. The increase in TGF-β2 gene expression can promote the transformation of hair follicle stem cells from the resting phase to the growth phase, thus being more beneficial for hair fixation.

[0139] Table 5 VEGFA expression results

[0140]

[0141]

[0142] As shown in Table 5 and Figure 5 as shown, after treatment with Component 1 (type XVII collagen) at the above concentration, the up-regulation rate of the VEGFA gene expression level was 128.16%, and after treatment with Component 2 (Platycladus orientalis extract) at the above concentration, the up-regulation rate of the VEGFA gene expression level was 63.79%; after treatment with Components 3-8 at the above concentration, the VEGFA gene expression level increased slightly, but the up-regulation rate did not exceed 23%.

[0143] After the treatments at the above concentrations in Examples 1-7, the expression level of VEGFA gene increased significantly, and the up-regulation rate ranged from 153.45% to 198.28%. It can be seen that the composition has a stronger ability to promote the expression of VEGFA gene compared with the single raw material components. Compared with Comparative Examples 1-5, Example 1 has a greater up-regulation rate of VEGFA gene expression. It can be seen that compared with other plant extracts, type XVII collagen and Platycladus orientalis extract have a better synergistic effect.

[0144] Effect Example 4: Test on the ability to reduce follicular senescent cells

[0145] 1. Test procedure

[0146] (1) Seed dermal papilla cells at a density of 1.5×10 5 cells per well in a 6-well plate and incubate overnight in a CO2 incubator (37 °C, 5% CO2).

[0147] (2) Administration and stimulation. According to the test grouping, administer drugs and stimulate simultaneously, with 3 replicates per group. The BC (blank control) group adds 2 mL of culture medium to each well, the NC (negative control) group adds 2 mL of culture medium containing H2O2 to each well, the PC (positive control) group adds 2 mL of culture medium containing H2O2 and WY14643 to each well, and the sample group adds culture medium containing H2O2 and the test sample at the corresponding concentration to each well. After that, transfer all 6-well plates to a CO2 incubator (37 °C, 5% CO2) and incubate for 24 hours.

[0148] (3) Use a β-galactosidase staining kit to stain the cells after treatment with the test substances, and count the proportion of senescent cells among all cells.

[0149] For each sample, take the average value of the test results of 3 replicates and record it in Table 6.

[0150] 2. Test results

[0151] Table 6 Test results of the ability to reduce follicular senescent cells

[0152]

[0153] The experimental results are shown in Table 6 and Figure 6 as follows. Compared with Components 1-8, Examples 1-7 have a significant improvement in inhibiting the senescence of dermal papilla cells. Compared with Comparative Examples 1-5, Example 1 has a stronger ability to inhibit the senescence of dermal papilla cells. It can be seen that compared with other plant extracts, type XVII collagen and Platycladus orientalis extract have a better synergistic effect.

[0154] Application Example 1: Preparation of hair care essence

[0155] According to the formula in Table 7, a hair care essence was prepared using the anti - hair loss and hair - strengthening composition of Example 1. The preparation process is as follows:

[0156] Raw material water and dipropylene glycol were respectively added to the main pot and stirred evenly. Then, type 17 collagen was added and stirred until completely dissolved. First, a certain amount of water was used to premix the Platycladus orientalis leaf extract, and after premixing evenly, it was added to the main pot and stirred evenly. Then, dipotassium glycyrrhizinate and VE derivative were added and stirred evenly. The pH of the material was tested, and arginine was added to adjust the pH to 6 - 7.

[0157] Table 7

[0158] Raw material Concentration Collagen type XVII 0.02％ Platycladus orientalis leaf extract 2.0％ Dipropylene glycol 5.0％ Dipotassium glycyrrhizinate 0.5％ VE derivative 0.4％ Arginine 1.0％ Water Balance

[0159] The anti - hair loss and hair - strengthening efficacy was tested through mouse experiments. Mice in the telogen phase (skin is pink) were selected for the experiment. Eighteen male SPF - level C57BL / 6 mice, 6 - 7 weeks old, were taken. After 7 days of adaptive feeding, the mice were randomly divided into 3 groups according to body weight, namely the normal control group, the positive control group, and the test article group, with 6 mice in each group. Under the anesthetized state of the mice, an area of about 2 cm × 3 cm on the back was selected as the depilated area, and the long side of this area was parallel to the spine. The hair in this area was shaved off using a hair clipper and further depilated using depilatory cream. After pre - hair removal, the mice in the test article group received the test article (applying the hair care essence of Example 1) by skin application, and the intervention period was 21 days. The mice in the normal control group were smeared with physiological saline, and the positive control group was smeared with a commercially available competitor product (5% minoxidil). The specific grouping and dosing information are shown in Table 8:

[0160] Table 8

[0161]

[0162]

[0163] Mice in each group were photographed daily before and after the intervention to record the skin and hair color of the depilated area. And the hair growth of the mice was scored on the 3rd, 7th, 10th, 14th, and 21st days before and after the intervention. The hair scoring criteria are shown in Table 9:

[0164] Table 9

[0165] Score Description 0 No hair growth was observed in the hair removal area 1 Hair growth in ≤ 20% area 2 Hair growth in 20% - 40% area 3 Hair growth in 40% - 60% area 4 Hair growth in 60% - 80% area 5 Hair growth in > 80% area

[0166] After the administration ended, the hair quality and hair length of the newly - grown hair in the depilated area of each group of mice were detected. After removing the newly - grown hair in the depilated area, it was weighed, and the hair quality was recorded per square centimeter of skin. In addition, 50 hairs were randomly selected from each mouse, fixed with transparent tape, and the length of the newly - grown hair in the depilated area was measured with a ruler, and the measurement results were recorded.

[0167] The test results showed that, compared with the normal control group and the positive control group, the mice using the hair care essence of the present invention had faster hair growth. By 14 days, hair growth had occurred in more than 80% of the area, and the hair was longer and heavier.

[0168] Although the specific embodiments of the present invention have been described above, those skilled in the art should understand that this is only an example. The protection scope of the present invention is defined by the appended claims. Without departing from the principle and essence of the present invention, those skilled in the art can make various changes or modifications to these embodiments, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A hair-fixing and anti-hair-loss composition, characterized in that, The anti - hair - loss and hair - strengthening composition comprises type XVII collagen and plant extracts, and the plant extracts include Platycladus orientalis extract.

2. The hair-fixing and anti-hair loss composition according to claim 1, wherein The molecular weight of the type XVII collagen is 20 - 200 kDa, such as 23.8 kDa, 97 kDa, 120 kDa or 180 kDa.

3. The hair-fixing and anti-hair loss composition according to claim 1, wherein The mass ratio of the type XVII collagen to the Platycladus orientalis extract is (0.0001 - 0.1):(0.1 - 5), such as 1:

100.

4. The hair-fixing and anti-hair-loss composition according to claim 1, wherein The anti - hair - loss and hair - strengthening composition consists of type XVII collagen and plant extracts; preferably, the anti - hair - loss and hair - strengthening composition consists of type XVII collagen and Platycladus orientalis extract.

5. The hair-fixing and anti-hair-loss composition according to claim 1, wherein The plant extracts further include plant extracts other than Platycladus orientalis extract, and the plant extracts are preferably selected from one or more of Magnolia officinalis extract, Polygonum multiflorum extract, Zingiber officinale extract, Salvia miltiorrhiza extract and Coffea arabica extract.

6. The hair-fixing and anti-hair loss composition according to claim 4, wherein The anti - hair - loss and hair - strengthening composition comprises type XVII collagen, Platycladus orientalis extract and Magnolia officinalis extract; preferably, the mass ratio of the type XVII collagen, Platycladus orientalis extract and Magnolia officinalis extract is (0.0001 - 0.1):(0.1 - 5):(0.1 - 2), such as 1:100:100; Or, the anti - hair - loss and hair - strengthening composition comprises type XVII collagen, Platycladus orientalis extract and Polygonum multiflorum extract; preferably, the mass ratio of the type XVII collagen, Platycladus orientalis extract and Polygonum multiflorum extract is (0.0001 - 0.1):(0.1 - 5):(0.1 - 10), such as 1:100:100; Or, the anti - hair - loss and hair - strengthening composition comprises type XVII collagen, Platycladus orientalis extract and Zingiber officinale extract; preferably, the mass ratio of the type XVII collagen, Platycladus orientalis extract and Zingiber officinale extract is (0.0001 - 0.1):(0.1 - 5):(0.1 - 10), such as 1:100:100; Or, the anti - hair - loss and hair - strengthening composition comprises type XVII collagen, Platycladus orientalis extract and Salvia miltiorrhiza extract; preferably, the mass ratio of the type XVII collagen, Platycladus orientalis extract and Salvia miltiorrhiza extract is (0.0001 - 0.1):(0.1 - 5):(0.1 - 6), such as 1:100:100; Or, the anti - hair - loss and hair - strengthening composition comprises type XVII collagen, Platycladus orientalis extract and Coffea arabica extract; preferably, the mass ratio of the type XVII collagen, Platycladus orientalis extract and Coffea arabica extract is (0.0001 - 0.1):(0.1 - 5):(0.0025 - 5), such as 1:100:2.

5.

7. The hair-fixing and anti-hair loss composition according to claim 1, wherein The anti - hair - loss and hair - strengthening composition further comprises niacinamide; Preferably, the mass ratio of the type XVII collagen, Platycladus orientalis extract and niacinamide is (0.0001 - 0.1):(0.1 - 5):(0.05 - 5), such as 1:100:

500.

8. An anti - hair - loss and hair - strengthening product, which comprises the anti - hair - loss and hair - strengthening composition according to any one of claims 1 - 7.

9. The hair strengthening and anti - hair loss product according to claim 8, wherein, The anti - hair - loss and hair - strengthening product is shampoo, hair conditioner, hair essence, body wash or body lotion.

10. Use of the hair-fixing and anti-hair loss composition according to any one of claims 1-7 in the preparation of a hair-fixing and anti-hair loss product.

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