Whitening and freckle-removing cosmetic composition based on badam extract as well as preparation method and application of whitening and freckle-removing cosmetic composition

Abstract

The invention belongs to the technical field of cosmetics, and discloses a whitening and freckle-removing cosmetic composition based on an almond extract as well as a preparation method and application of the whitening and freckle-removing cosmetic composition. The whitening and freckle-removing cosmetic composition is prepared from the following components in percentage by weight: 0.5 to 5 percent of an almond oligosaccharide extract, 1 to 3 percent of vitamin C ethyl ether, 0.1 to 0.5 percent of dipotassium glycyrrhizinate, 0.1 to 0.3 percent of sodium hyaluronate and the balance of a cosmetic carrier, the adopted extract active component is high-purity Badam oligosaccharide obtained through an innovative extraction process, and the mechanism of the Badam oligosaccharide for removing freckles and whitening by inhibiting the activity of tyrosinase is verified for the first time; meanwhile, by combining the synergistic interaction of the compound components, the safety and efficacy of the cosmetic composition are remarkably improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a whitening and freckle-removing cosmetic composition based on almond extract, and a preparation method and application thereof. Background Art

[0002] Traditional whitening cosmetics often rely on small molecule compounds (such as hydroquinone, arbutin, etc.). Although they are effective, there are problems such as irritation and allergy risks. In recent years, natural plant extracts have attracted much attention due to their safety and efficacy. However, in the prior art, the application of almond extract is mostly limited to food or basic moisturization, and its whitening activity has not been fully explored. For example, Patent CN102907603A discloses a composite microcapsule powder containing almond extract, but does not involve whitening efficacy. In addition, the existing preparation process of almond extract is complex, and the purity of active components is insufficient, resulting in poor product stability and limited efficacy.

[0003] The present invention obtains high-purity almond oligosaccharide (molecular weight 1070Da, composed of glucose-galactose) through an innovative extraction process (supercritical CO2 degreasing combined with gradient chromatography purification), and for the first time verifies its mechanism of removing freckles and whitening by inhibiting the activity of tyrosinase. At the same time, combined with compound components (such as vitamin C derivatives, dipotassium glycyrrhizinate) to synergistically enhance the effect, significantly improving the safety and efficacy of the product. Summary of the Invention

[0004] The purpose of the present invention is to solve the above-mentioned technical defects existing in the existing whitening cosmetics, and to provide a whitening and freckle-removing cosmetic composition based on almond extract, and a preparation method and application thereof.

[0005] To achieve the above purpose, the present invention adopts the following technical solutions:

[0006] The first aspect of the present invention is to provide a whitening and freckle-removing cosmetic composition based on almond extract, comprising the following components in weight percentage: 0.5-5% of almond oligosaccharide extract, 1-3% of vitamin C ethyl ether, 0.1-0.5% of dipotassium glycyrrhizinate, 0.1-0.3% of sodium hyaluronate, and the balance is a cosmetic carrier.

[0007] Preferably, the almond oligosaccharide extract is prepared by the following method:

[0008] a. Crushing almond kernels and subjecting them to supercritical CO2 degreasing to obtain degreased powder;

[0009] b. Adding water to extract the degreased powder at a solid-liquid ratio of 1:10-1:30, extracting at 20-90°C for 2 times, each time for 1-2 hours, and combining the filtrates;

[0010] c. Concentrate to 1 / 5 - 1 / 6 of the original volume, add 4 times the volume of 95% ethanol for alcohol precipitation, take the supernatant and freeze-dry to obtain the crude extract;

[0011] d. After the crude extract is purified by AB-8 macroporous resin, it is successively passed through Sephadex G-25 and G-50 gel column chromatography, and freeze-dried to obtain a almond oligosaccharide extract with a purity ≥ 95%.

[0012] More preferably, in step a, the supercritical CO2 extraction pressure is 25 - 35 MPa and the temperature is 40 - 50 °C.

[0013] Preferably, the cosmetic carrier is an oil-in-water emulsion, gel or essence matrix.

[0014] Preferably, the whitening and freckle-removing cosmetic composition further comprises 0.1 - 0.5% of niacinamide.

[0015] The second aspect of the present invention is to provide a whitening and freckle-removing facial mask, comprising the whitening and freckle-removing cosmetic composition as described in any one of the above and a non-woven fabric substrate.

[0016] The third aspect of the present invention is to provide an application of the almond oligosaccharide extract prepared as described in any one of the above in the preparation of a cosmetic for inhibiting tyrosinase activity.

[0017] The fourth aspect of the present invention is to provide a preparation method of a whitening and freckle-removing lotion, comprising the following steps:

[0018] a. Heat the oil phase (squalane, jojoba oil) to 75 °C;

[0019] b. Heat the water phase (almond oligosaccharide extract, glycerol, sodium hyaluronate) to the same temperature;

[0020] c. Slowly add the water phase to the oil phase for homogenizing and emulsifying, and add a preservative after cooling.

[0021] Preferably, in step c, the homogenizing and emulsifying speed is 3000 - 5000 rpm and the time is 5 - 10 minutes.

[0022] The fifth aspect of the present invention is to provide a preparation method of a whitening and freckle-removing essence, mixing the almond oligosaccharide extract prepared as described in any one of the above with 1,3-propanediol and panthenol, followed by high-pressure homogenization and filtration sterilization.

[0023] The present invention adopts the above technical solutions, compared with the prior art, has the following technical effects:

[0024] (1) Extraction process optimization: Supercritical CO2 degreasing is used to reduce lipid interference, and gradient chromatography purification is adopted to obtain high-purity oligosaccharides (purity ≥ 95%), with a molecular weight of 1070 Da, composed of glucose and galactose;

[0025] (2) Efficacy verification: It is confirmed through cell experiments that almond oligosaccharide extract can inhibit tyrosinase activity (IC 50 = 0.8 mg / mL), which is superior to arbutin (IC 50 = 1.2 mg / mL);

[0026] (3) Synergistic compounding: Ethyl ether of vitamin C and dipotassium glycyrrhizinate enhance the antioxidant and anti-inflammatory effects of the whitening and freckle-removing cosmetic composition, reduce melanin deposition, and significantly improve the safety and efficacy of the product. Detailed implementation mode

[0027] In the present invention, high-purity almond oligosaccharides (with a molecular weight of 1070 Da, composed of glucose-galactose) are obtained through an innovative extraction process (supercritical CO2 degreasing combined with gradient chromatography purification), and for the first time, its mechanism of removing freckles and whitening by inhibiting tyrosinase activity is verified. At the same time, combined with compounding components (such as vitamin C derivatives, dipotassium glycyrrhizinate) for synergistic effect, the safety and efficacy of the product are significantly improved.

[0028] The present invention is introduced in detail and specifically through specific embodiments below to better understand the present invention, but the following embodiments do not limit the scope of the present invention.

[0029] Example 1: Extraction of almond oligosaccharides

[0030] a. Take almond kernels from Shache County, Xinjiang, crush them and degrease them with supercritical CO2 at 30 MPa and 45 °C;

[0031] b. Add water to the degreased powder according to a solid-liquid ratio of 1:20, extract at 80 °C for 2 times, 1.5 hours each time;

[0032] c. Combine the filtrates and concentrate to 1 / 5 of the original volume, add 4 times the volume of 95% ethanol for alcohol precipitation, and take the supernatant for freeze-drying;

[0033] d. After purifying the crude extract with AB-8 resin, pass it through Sephadex G-25 and G-50 columns in sequence, collect the main peak and freeze-dry it to obtain a white powder (purity 97.2%), namely almond oligosaccharide extract.

[0034] Example 2: Preparation of whitening essence

[0035] It contains the following components in weight percentages: 3% of almond oligosaccharide extract, 2% of ethyl ether of vitamin C, 0.3% of dipotassium glycyrrhizinate, 5% of 1,3-propanediol, and the balance is water.

[0036] After mixing and dissolving the above components, sterilize through a 0.22 μm filter membrane, and then fill into containers to obtain the product.

[0037] Example 3: Preparation of Freckle-Removing Lotion

[0038] a. Oil phase: Mix 8% squalane and 5% jojoba oil evenly, and set aside.

[0039] b. Aqueous phase: Mix 2% almond oligosaccharide extract, 5% glycerol, and 0.2% sodium hyaluronate evenly, and set aside.

[0040] c. Heat the oil phase and the aqueous phase to 75 °C respectively, homogenize and emulsify (4000 rpm, 8 minutes), and add the preservative phenoxyethanol after cooling.

[0041] Comparative Example 1: Traditional Water Extraction Method

[0042] Different from Example 1, supercritical CO2 degreasing and gel chromatography were not used, and the rest was the same as Example 1. The purity of the almond oligosaccharide extract obtained was only 65%, and the whitening effect decreased by 40%.

[0043] Comparative Example 2: Single Component

[0044] Different from Example 2, it only contained almond oligosaccharide extract, sodium hyaluronate and a cosmetic carrier (without vitamin C ethyl ether and dipotassium glycyrrhizinate), and the tyrosinase inhibition rate decreased by 25%.

[0045] Comparative Example 3: Commercially Available Arbutin Essence

[0046] The irritation test showed that 30% of the subjects of the commercially available arbutin essence had erythema, while the composition of the present invention had no adverse reactions.

[0047] In summary, the whitening and freckle-removing cosmetic composition based on almond extract of the present invention, its preparation method and application combine supercritical extraction, gradient purification and compound synergistic technology, significantly improving the whitening efficacy and safety of almond extract, meeting the requirements of the "Regulations on the Supervision and Administration of Cosmetics" and the "Measures for the Administration of Cosmetics Registration and Filing", and adjusting the parameters according to the actual production conditions during specific implementation, and completing the toxicology and stability tests.

[0048] The specific embodiments of the present invention have been described in detail above, but they are only examples, and the present invention is not limited to the specific embodiments described above. For those skilled in the art, any equivalent modifications and substitutions to the present invention are also within the scope of the present invention. Therefore, all equivalent transformations and modifications made without departing from the spirit and scope of the present invention should be covered within the scope of the present invention.

Claims

1. A whitening and freckle-removing cosmetic composition based on almond extract, characterized in that, Comprising the following components in weight percentages: almond oligosaccharide extract 0.5 - 5%, vitamin C ethyl ether 1 - 3%, dipotassium glycyrrhizinate 0.1 - 0.5%, sodium hyaluronate 0.1 - 0.3%, and the balance being a cosmetic carrier.

2. The whitening and freckle-removing cosmetic composition according to claim 1, wherein The almond oligosaccharide extract is prepared by the following method: a. Crushing almond kernels and subjecting them to supercritical CO2 degreasing to obtain degreased powder; b. Adding water to extract the degreased powder at a solid - liquid ratio of 1:10 - 1:30, extracting at 20 - 90°C for 2 times, each time for 1 - 2 hours, and combining the filtrates; c. Concentrating to 1 / 5 - 1 / 6 of the original volume, adding 4 times the volume of 95% ethanol for alcohol precipitation, and taking the supernatant for freeze - drying to obtain a crude extract; d. After purifying the crude extract through AB - 8 macroporous resin, successively passing through Sephadex G - 25 and G - 50 gel column chromatography, and freeze - drying to obtain an almond oligosaccharide extract with a purity ≥ 95%.

3. The whitening and freckle-removing cosmetic composition according to claim 1, characterized in that, In step a, the supercritical CO2 extraction pressure is 25 - 35 MPa, and the temperature is 40 - 50°C.

4. The whitening and freckle-removing cosmetic composition according to claim 1, wherein The cosmetic carrier is an oil - in - water emulsion, gel, or essence matrix.

5. The whitening and freckle-removing cosmetic composition according to claim 1, wherein It also contains 0.1 - 0.5% of niacinamide.

6. A whitening and freckle-removing facial mask, characterized in that, Comprising the whitening and freckle - removing cosmetic composition according to any one of claims 1 to 5 and a non - woven fabric substrate.

7. Use of the almond oligosaccharide extract prepared according to claim 2 or 3 in the preparation of a cosmetic for inhibiting tyrosinase activity.

8. A method for preparing a whitening and freckle-removing lotion, characterized in that, Including the following steps: a. Heating the oil phase (squalane, jojoba oil) to 75°C; b. Heating the water phase (almond oligosaccharide extract, glycerol, sodium hyaluronate) to the same temperature; c. Slowly adding the water phase to the oil phase for homogenizing emulsification, and adding a preservative after cooling.

9. The preparation method according to claim 8, characterized in that, In step c, the homogenizing emulsification speed is 3000 - 5000 rpm, and the time is 5 - 10 minutes.

10. Preparation method of a whitening and freckle-removing essence, characterized in that Mixing the almond oligosaccharide extract prepared according to claim 2 or 3 with 1,3 - propanediol and panthenol, followed by high - pressure homogenization and filtration sterilization.