Cream for promoting growth of pet hair and preparation method thereof

Abstract

The invention relates to a cream for promoting pet hair growth and a preparation method of the cream, and relates to the technical field of pet biological medicine, and the cream comprises the following preparation raw materials by mass: 8-10% of microencapsulated fish oil; 6%-12% of blood protein peptide; 3%-5% of silkworm chrysalis protein powder; 2%-5% of hydrolyzed chicken liver powder; 1%-3% of a yeast extract; 0.005% to 0.01% of a rosemary extract; 0.1%-0.4% of an antioxidant; wherein the blood protein peptide is selected from one or more of an MLL peptide fragment and a WDL peptide fragment; the amino acid sequence of the MLL peptide fragment is methionine-leucine-leucine, and the amino acid sequence of the MLL peptide fragment is methionine-leucine-leucine; the amino acid sequence of the WDL peptide fragment is tryptophan-aspartic acid-leucine. The pet food has synergistic and long-term stable effects, is suitable for pets such as dogs and cats, is safe and free of side effects, and can be produced on a large scale.

Description

Technical Field

[0001] This application relates to the technical field of pet biomedicine, and particularly to a cream for promoting pet hair growth and its preparation method. Background Art

[0002] The health of pet hair is an important indicator to measure its overall nutritional status and skin function. Hair quality not only affects the appearance of pets, but is also closely related to the integrity of the skin barrier, immune regulation and microcirculation status. In recent years, with the increase in the penetration rate of pet families (the annual compound growth rate of the global pet care market reaches 6.8%), consumers' demand for pet hair care products has upgraded from basic cleaning to functional and precise, especially focusing on effects such as hair loss repair, hair follicle activation and anti-inflammatory soothing. However, the existing technical solutions still have significant defects in ingredient design, mechanism of action and long-term safety, specifically manifested as follows: Lack of ingredient diversity and synergistic effect: Most commercially available products rely on a single active ingredient (such as Omega-3 in fish oil, vitamin E or biotin). Although they can partially improve hair luster, they cannot systematically solve deep problems such as hair follicle atrophy and microcirculation disorders. For example, fish oil cream only promotes hair growth by supplementing unsaturated fatty acids, but experiments show that its transdermal absorption rate is less than 15%, and it has no significant effect on the proliferation of hair follicle stem cells. In addition, single ingredients are prone to inactivation due to oxidation (such as the peroxide value of fish oil increasing by more than 30% during storage), resulting in poor product stability.

[0003] Limited application of blood peptide components: Bioactive peptides derived from blood (such as blood peptide concentrate) have shown potential in the field of human hair growth, but their application in pet care is still in its infancy.

[0004] Inefficient transdermal delivery system: The skin structure of pets is significantly different from that of humans (such as a neutral pH and a complex lipid composition). Traditional cream matrices (such as petrolatum) can moisturize, but they hinder the penetration of active ingredients. In addition, chemical penetration enhancers (such as propylene glycol) may cause skin irritation reactions in pets (Draize score ≥ 2), limiting long-term use.

[0005] Therefore, there is an urgent need in this field to develop a cream for promoting pet hair growth to fill the gap in precise pet hair follicle repair technology. Summary of the Invention

[0006] The purpose of this application is to provide a cream for promoting pet hair growth and its preparation method. Through scientific formulation and advanced preparation technology, the cream of this application has the functions of nutritional supplement, antioxidant and hair follicle activation, is applicable to pets such as dogs and cats, is safe without side effects, and can be mass-produced.

[0007] In a first aspect, the present application provides a cream for promoting pet hair growth, comprising preparation raw materials in the following mass percentages: Microencapsulated fish oil: 8% - 10%; Blood protein peptide: 6% - 12%; Silkworm pupa protein powder: 3% - 5%; Hydrolyzed chicken liver powder: 2% - 5%; Yeast extract: 1% - 3%; Rosemary extract: 0.005% - 0.01%; Antioxidant: 0.1% - 0.4%; Among them, the blood protein peptide is selected from one or more of the MLL peptide segment and the WDL peptide segment; the amino acid sequence of the MLL peptide segment is methionine - leucine - leucine; the amino acid sequence of the WDL peptide segment is tryptophan - aspartic acid - leucine.

[0008] Optionally, the blood protein peptide is composed of the MLL peptide segment and the WDL peptide segment.

[0009] Optionally, the antioxidant is composed of d-biotin and vitamin E.

[0010] Optionally, the cream further comprises preparation raw materials in the following mass percentages: Oil phase matrix: 10% - 20%; Aqueous phase matrix: 60% - 70%; Emulsifier: 3% - 6%.

[0011] Optionally, the oil phase matrix is composed of glyceryl monostearate and white petrolatum.

[0012] Optionally, the aqueous phase matrix is composed of carbomer, glycerol and water.

[0013] Optionally, the emulsifier is one or several of sodium dodecyl sulfate, polyethylene glycol glycerol caprylate / caprate, and polyoxyethylene (6) glycerol laurate.

[0014] In a second aspect, the present application provides a preparation method for a cream for promoting pet hair growth, comprising the following steps: S1: Preparation of the oil phase: Heat and melt glyceryl monostearate, white petrolatum, and microencapsulated fish oil; S2: Preparation of the aqueous phase: Dissolve sodium dodecyl sulfate, carbomer, and glycerol in water and heat and mix; S3: Preparation of the active phase: Dissolve the blood protein peptide in water and homogenize it with hydrolyzed chicken liver powder and silkworm pupa protein powder; S4: Preparation of the cream: Slowly add the oil phase to the water phase, stir evenly, add the active phase, yeast extract, antioxidant and rosemary extract after cooling, homogenize, adjust the pH to 6.5 - 7.0, and then homogenize and fill to obtain the finished product.

[0015] In summary, this application includes at least one of the following beneficial technical effects: 1. Synergistic effect: The combination of microencapsulated fish oil (rich in Omega-3) and blood peptide concentrate (promoting hair follicle nutrient absorption) enhances hair follicle activity. 2. Natural and safe: Silkworm pupa protein powder and hydrolyzed chicken liver powder provide essential amino acids, and rosemary extract replaces chemical preservatives. 3. High stability: The microencapsulation technology protects the active ingredients, and vitamin E and d-biotin work together to delay oxidation. There is no crystallization after storing at room temperature for 12 months. Specific embodiments

[0016] The present invention will be further described below in conjunction with specific embodiments.

[0017] Preparation example: Blood protein peptide Screen out blood protein peptides from bovine blood protein solution after enzymatic hydrolysis, namely WDL (TrpAspLeu) peptide segment and MLL (MetLeuLeu) peptide segment. Use an automatic peptide synthesizer to synthesize the WDL peptide segment and MLL peptide segment respectively by solid-phase synthesis method (synthesized by Nanjing Yuanpeptide Biotechnology Co., Ltd.), purify them by high performance liquid chromatography (HPLC), and identify the synthesized peptides by methods such as mass spectrometry (MS) and amino acid sequence analysis to confirm their sequences and purities. Finally, obtain blood protein peptides with a purity greater than 95%.

[0018] Example 1: A cream for promoting pet hair growth The raw material composition of a cream for promoting pet hair growth: Microencapsulated fish oil: 8%; Blood protein peptide MLL peptide segment: 1%; Blood protein peptide WDL peptide segment: 1%; Silkworm pupa protein powder: 5%; Hydrolyzed chicken liver powder: 4%; Yeast extract: 2%; d-biotin: 0.06%; Vitamin E: 0.34%; Rosemary extract: 0.01%; Oil phase matrix glyceryl monostearate 2%; Oil phase matrix white petrolatum 8%; Emulsifier sodium dodecyl sulfate 4%; Aqueous phase matrix carbomer 2%; Aqueous phase matrix glycerol 3%; The rest is aqueous phase matrix water, making up to 100%.

[0019] The preparation method of the above cream includes the following steps: S1: Preparation of the oil phase: Heat glyceryl monostearate, white petrolatum, and microencapsulated fish oil to 70°C until melted. S2: Preparation of the aqueous phase: Dissolve sodium dodecyl sulfate, carbomer, and glycerol in water and heat to 50°C for mixing. S3: Preparation of the active phase: Dissolve the MLL peptide segment and the WDL peptide segment in water, and homogenize them with hydrolyzed chicken liver powder and silkworm pupa protein powder; S4: Preparation of the cream: Slowly add the oil phase to the water phase, stir at 1300 r / min for 20 minutes, cool to 40 °C, then add the active phase, yeast extract, d-biotin, vitamin E and rosemary extract, conduct homogenization treatment, adjust the pH to 6.5 - 7.0, and perform homogenization filling to obtain the finished product.

[0020] Example 2: A cream for promoting pet hair growth Raw material composition of a cream for promoting pet hair growth: Microencapsulated fish oil: 10%; Blood protein peptide MLL peptide segment: 3%; Blood protein peptide WDL peptide segment: 3%; Silkworm pupa protein powder: 3%; Hydrolyzed chicken liver powder: 2%; Yeast extract: 1%; d-biotin: 0.015%; Vitamin E: 0.085%; Rosemary extract: 0.005%; Oil phase matrix glyceryl monostearate 2%; Oil phase matrix white petrolatum 8%; Emulsifier sodium dodecyl sulfate 4%; Aqueous phase matrix carbomer 2%; Aqueous phase matrix glycerol 3%; The rest is the aqueous phase matrix water, making up to 100%.

[0021] The preparation method is the same as that of Example 1.

[0022] Comparative Example 1: A cream for promoting pet hair growth Raw material composition of a cream for promoting pet hair growth: Silkworm pupa protein powder: 3%; Hydrolyzed chicken liver powder: 2%; Yeast extract: 1%; d-biotin: 0.015%; Vitamin E: 0.085%; Rosemary extract: 0.005%; Oil phase matrix glyceryl monostearate 2%; Oil phase matrix white petrolatum 8%; Emulsifier sodium dodecyl sulfate 4%; Aqueous phase matrix carbomer 2%; Aqueous phase matrix glycerol 3%; The rest is the aqueous phase matrix water, making up to 100%.

[0023] The preparation method is the same as that of Example 1.

[0024] Example 3: Long-term stability study of the cream Experimental conditions: High-temperature experiment: Place in a constant temperature oven at 60 °C for 10 days, and take samples for testing on the 0th, 5th, and 10th days.

[0025] High-humidity experiment: Place under the condition of relative humidity 90% ± 5% for 10 days, and the sampling time is the same as above.

[0026] Strong light experiment: Place under the light intensity of 4500 lx ± 500 lx for 10 days, and the sampling time is the same as above.

[0027] Long-term experiment: Store for 12 months under the conditions of 25°C ± 2°C and relative humidity of 60% ± 10%, and sample for testing at the end of 0, 3, 6, 9, and 12 months.

[0028] Detection indicators: Physical stability: Appearance (color, uniformity), layering, crystallization phenomenon.

[0029] Chemical stability: Content of MLL / WDL peptide segments (HPLC method, C18 chromatographic column, mobile phase: gradient elution of 0.1M phosphate buffer - acetonitrile), oxidation rate of vitamin E (iodometric method).

[0030] The experimental results are shown in Table 1: Table 1 Conclusion: Retention rate of MLL / WDL peptide segment content > 95%, oxidation rate of vitamin E < 5%; The cream is stable within 12 months under conventional storage conditions (25°C) and meets the pharmaceutical standards.

[0031] Example 4: Animal pharmacodynamic experiment Select C57BL / 6 hair-loss mice (dexamethasone-induced hair-loss model), divided into 4 groups (n = 10): Control group: Blank control group (Comparative Example 1), Low-dose group: Cream containing 3% of each MLL / WDL peptide segment (Example 1), High-dose group: Cream containing 6% of each MLL / WDL peptide segment (Example 2), Positive control group: Commercially available fish oil cream. Administration method: Apply locally once a day for 6 consecutive weeks. Detection indicators: Hair regeneration rate: Proportion of hair coverage area on the skin surface, Hair follicle density: Count the number of hair follicles per square millimeter by tissue section staining (H&E).

[0032] The experimental results are shown in Table 2: Group Hair regeneration rate <![CDATA[Hair follicle density (number / mm 2 )]]> Blank control group 12％±3％ 8.2±1.5 Low-dose group 58％±7％ 22.4±3.1* High-dose group 82％±9％ 34.6±4.5** Positive control group 32％±5％ 12.3±2.3 *Note: **P < 0.05, *P < 0.01 (compared with the blank control group) Conclusion: The hair regeneration rate in the high-dose group increased by 82%, and the hair follicle density increased by 3.2 times, which is significantly better than the commercially available product, confirming that the cream has the function of promoting hair follicle repair.

[0033] Those skilled in the art will further recognize that the present invention can be implemented in other specific forms without departing from its spirit or central characteristics. Since the foregoing description of the present disclosure only discloses its exemplary embodiments, it should be understood that other variations are considered to be within the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments described in detail herein. Instead, reference should be made to the appended claims to indicate the scope and content of the present invention.

Claims

1. A cream for promoting pet hair growth, characterized in that, The preparation raw materials include the following mass percentages: Microencapsulated fish oil: 8% - 10%; Blood protein peptide: 6% - 12%; Silkworm pupa protein powder: 3% - 5%; Hydrolyzed chicken liver powder: 2% - 5%; Yeast extract: 1% - 3%; Rosemary extract: 0.005% - 0.01%; Antioxidant: 0.1% - 0.4%; Among them, the blood protein peptide is selected from one or more of the MLL peptide segment and the WDL peptide segment; the amino acid sequence of the MLL peptide segment is methionine - leucine - leucine; the amino acid sequence of the WDL peptide segment is tryptophan - aspartic acid - leucine.

2. The cream for promoting pet hair growth according to claim 1, characterized in that, The blood protein peptide is composed of the MLL peptide segment and the WDL peptide segment.

3. The cream for promoting pet hair growth according to claim 1, characterized in that, The antioxidant is composed of d - biotin and vitamin E.

4. The cream preparation for promoting pet hair growth according to claim 1, characterized in that, The cream preparation also includes the following mass percentages of preparation raw materials: Oil phase matrix: 10% - 20%; Aqueous phase matrix: 60% - 70%; Emulsifier: 3% - 6%.

5. The cream for promoting pet hair growth according to claim 4, characterized in that, The oil phase matrix is composed of glycerol monostearate and white petrolatum.

6. The cream for promoting pet hair growth according to claim 4, wherein The aqueous phase matrix is composed of carbomer, glycerol and water.

7. A cream for promoting pet hair growth according to claim 4, characterized in that, The emulsifier is one or several of sodium dodecyl sulfate, polyethylene glycol glycerol caprylate / caprate, and lauroyl polyoxyethylene (6) glycerol ester.

8. A method for preparing the cream according to any one of claims 1-7, characterized in that, It includes the following steps: S1: Preparation of the oil phase: Heat and melt glycerol monostearate, white petrolatum, and microencapsulated fish oil. S2: Preparation of the aqueous phase: Dissolve sodium dodecyl sulfate, carbomer, and glycerol in water and heat and mix. S3: Preparation of the active phase: Dissolve the blood protein peptide in water and homogenize it with hydrolyzed chicken liver powder and silkworm pupa protein powder. S4: Preparation of the cream: Slowly add the oil phase to the aqueous phase, stir evenly, cool down, then add the active phase, yeast extract, antioxidant, and rosemary extract, conduct homogenization treatment, adjust the pH to 6.5 - 7.0, and perform homogenization filling to obtain the finished product.

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