Functional organic fertilizer prepared from roxburgh rose residues
By mixing and fermenting prickly pear residue, Chinese medicine residue and rice husk powder, functional organic fertilizers are prepared by using Aspergillus rosy and Trichoderma Corning, the problem of waste of resources of prickly pear residue and Chinese medicine residue is solved, and the effective utilization of nutrients and the promotion of crop growth is achieved.
Patent Information
- Application Number
- CN202510431869.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-08
- Publication Date
- 2025-07-08
AI Technical Summary
The prickly pear residue and Chinese medicine residue are not effectively utilized during the processing process, resulting in waste of resources. The existing Chinese medicine residue extraction methods are inefficient, making it difficult to make full use of their nutrients.
Mix the prickly pear residue, Chinese medicine residue and rice husk powder as fermentation base, and use a mixed fermentation bacterial solution of Aspergillus rutheniae and Trichoderma Corning for compost fermentation to prepare functional organic fertilizers to promote crop growth.
Through the mixing and fermentation treatment of specific proportions, the prepared functional organic fertilizer can effectively promote crop growth, make up for the deficiency of insufficient nutrients of prickly slag, and improve the fertilizer efficiency of the fertilizer.
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of the preparation of organic fermentation fertilizers, and particularly relates to a functional organic fertilizer prepared from Rosa roxburghii Tratt residue. Background Art
[0002] Rosa roxburghii Tratt is a wild fruit growing in the southwestern region of China. In recent years, it has gradually attracted attention due to its rich nutritional components and unique flavor, and has become an emerging product in the fruit market. The fruit of Rosa roxburghii Tratt is rich in vitamin C, amino acids, minerals and various beneficial plant compounds, and has high health care value. Therefore, the cultivation and processing of Rosa roxburghii Tratt have gradually emerged. In the processing of Rosa roxburghii Tratt, the main products are Rosa roxburghii Tratt juice, Rosa roxburghii Tratt dried fruits and Rosa roxburghii Tratt jam, etc. However, these processing processes will produce a large number of by-products, namely Rosa roxburghii Tratt residue. Rosa roxburghii Tratt residue refers to the solid residue left after the juice extraction or other processing of Rosa roxburghii Tratt fruits, and contains nutrients such as cellulose, pectin, plant protein and trace elements. Although Rosa roxburghii Tratt residue contains these nutrients, due to the low content, it is difficult to be directly utilized, so the utilization efficiency in actual production is low, and it is often discarded as industrial waste, resulting in waste of resources.
[0003] Traditional Chinese medicine residue comes from the production of traditional Chinese medicine raw materials, the processing and preparation of traditional Chinese medicine decoction pieces, and the production of light chemical products containing traditional Chinese medicine components, etc. Among them, the amount of residue brought by the production of traditional Chinese patent medicine is the largest. Traditional Chinese medicine is mainly composed of plant, animal and part of mineral medicinal materials, and the plant medicinal materials account for more than 87%. At present, the production and processing of traditional Chinese medicinal materials in China are still in the primary stage. The extraction means are relatively single, and the extraction efficiency is relatively low. The traditional Chinese medicine residue remaining after extraction generally contains a large amount of crude protein, crude fat, cellulose, hemicellulose, lignin, amino acids, polysaccharides, oils, vitamins, organic acids and some nutrients necessary for the human body. The main reason for the rich nutrients in traditional Chinese medicine residue is due to the limited choice of modern pharmaceutical processes. Most pharmaceutical factories only utilize a small part of the effective components, so the traditional Chinese medicine residue after extraction still retains a large amount of chemical components and has extremely high development value.
[0004] Therefore, it is of great significance to prepare a functional organic fertilizer by mixing and fermenting Rosa roxburghii Tratt residue and traditional Chinese medicine residue to realize the resource utilization of Rosa roxburghii Tratt residue and traditional Chinese medicine residue. Summary of the Invention
[0005] According to the deficiencies of the prior art, the present invention uses Rosa roxburghii Tratt residue, traditional Chinese medicine residue and rice husk powder as the fermentation base materials for preparing the functional organic fertilizer. Through a specific ratio of mixing, and then spraying the constructed mixed fermentation bacterial liquid on the fermentation base materials, the functional organic fertilizer is obtained through composting fermentation. When used for fertilizing crops, it can promote the growth of crops, and thus solve the technical problems proposed in the background art. Specifically, the technical solution of the present invention includes the following contents:
[0006] A functional organic fertilizer prepared from Rosa roxburghii Tratt residue, and the preparation method of the functional organic fertilizer comprises the following steps:
[0007] The Rosa roxburghii Tratt residue, traditional Chinese medicine residue and rice straw powder are mixed to form a fermentation base material, and the mixed fermentation bacterial liquid is sprayed onto the fermentation base material and then wetted with water to form a fermentation base material to be fermented;
[0008] The fermentation base material is sequentially subjected to composting fermentation and drying treatment to obtain the functional organic fertilizer.
[0009] Furthermore, the preparation method of the mixed fermentation bacterial liquid comprises the following steps:
[0010] Monascus purpureus and Trichoderma koningii are respectively inoculated into solid culture dishes and placed in an incubator at 30 °C for 24 h to obtain a Monascus purpureus spore culture dish and a Trichoderma koningii spore culture dish;
[0011] The Monascus purpureus spore culture dish and the Trichoderma koningii spore culture dish are rinsed with sterile water and the rinsing solutions are combined to obtain a mixed spore suspension;
[0012] The mixed spore suspension is inoculated into a liquid culture medium and cultured to obtain a mixed fermentation bacterial liquid.
[0013] Furthermore, the strain number of the Monascus purpureus is CICC 40712.
[0014] Furthermore, the strain number of the Trichoderma koningii is CICC 13025.
[0015] Furthermore, the preparation method of the solid culture dish comprises the following steps:
[0016] Corn steep liquor powder, peptone, agar and purified water are mixed in a ratio of 7-9:13-15:14:950-970 by weight, heated to 50 °C - 55 °C and stirred until completely dissolved, and then sterilized at 121 °C for 20 min - 30 min to obtain a solid culture dish.
[0017] Furthermore, the dosage of the sterile water rinsing is controlled at 20 ml - 30 ml.
[0018] Furthermore, the inoculation amount of the mixed spore suspension inoculation is 0.005 - 0.01 of the total weight of the liquid culture medium.
[0019] Furthermore, the preparation method of the liquid culture medium comprises the following steps:
[0020] Whey powder, ammonium sulfate, potassium dihydrogen phosphate, brown sugar and purified water are mixed in a weight ratio of 15-20:0.8-1:1.5-2:5-7:980-1000, heated to 50°C - 55°C and stirred until completely dissolved, and then sterilized at 121°C for 20 min - 30 min to obtain a liquid medium.
[0021] Further, the weight ratio of the mixed fermentation bacterial liquid to the fermentation base material is 1:80 - 100.
[0022] Further, the water content of the base material to be fermented is 55% - 65%.
[0023] Further, the conditions for compost fermentation include a compost height of 1 m - 1.5 m, a fermentation time of 8 days - 10 days, a turning frequency of once every 2 days, and a ventilation rate of 0.4 L / min - 0.5 L / min.
[0024] Compared with the prior art, the beneficial effects of the present invention are as follows:
[0025] By designing the composition components, the present invention uses Eucommia ulmoides residue, Astragalus membranaceus residue and Coptis chinensis residue to form traditional Chinese medicine residues. Eucommia ulmoides residue contains crude protein, crude fat and trace elements such as calcium, phosphorus and potassium. Astragalus membranaceus residue contains astragalus polysaccharide, amino acids and trace elements such as zinc and iron. Coptis chinensis residue contains alkaloids, flavonoids and organic acids, etc., which can make up for the deficiency of nutrients in Rosa roxburghii Tratt residue and improve the fertilizer efficiency of the fermented fertilizer. The traditional Chinese medicine residues, Rosa roxburghii Tratt residue and rice straw powder are mixed in a specific ratio to form a fermentation base material, and then the mixed fermentation bacterial liquid composed of Monascus purpureus and Trichoderma koningii is sprayed on the fermentation base material. Through the synergistic decomposition and fermentation of Monascus purpureus and Trichoderma koningii, the prepared functional organic fertilizer can promote the growth of crops when used for fertilizing crop growth. Specific Embodiments
[0026] The technical solutions of the present invention will be clearly and completely described below through the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present invention.
[0027] Unless otherwise specified, the raw materials and reagents used in the present invention below are all commercially available products or can be prepared by known methods.
[0028] Preparation Example 1:
[0029] The preparation method of a solid culture dish specifically includes the following process:
[0030] Weigh 7 parts by weight of corn steep liquor dry powder, 13 parts by weight of peptone, and 14 parts by weight of agar and add them to a beaker. Then add 950 parts by weight of purified water, mix and stir. Then place it in a water bath and heat to 50 °C, and stir at a speed of 200 r / min until all the above materials are dissolved. Then take it out and place it in an autoclave, heat up to 121 °C and sterilize for 20 min. After sterilization, pour the medium into a petri dish while it is still hot on a clean bench, and let it cool and solidify naturally to obtain a solid petri dish.
[0031] Preparation Example 2:
[0032] A method for preparing a solid petri dish, specifically including the following process:
[0033] Weigh 8 parts by weight of corn steep liquor dry powder, 14 parts by weight of peptone, and 14 parts by weight of agar and add them to a beaker. Then add 960 parts by weight of purified water, mix and stir. Then place it in a water bath and heat to 50 °C, and stir at a speed of 200 r / min until all the above materials are dissolved. Then take it out and place it in an autoclave, heat up to 121 °C and sterilize for 20 min. After sterilization, pour the medium into a petri dish while it is still hot on a clean bench, and let it cool and solidify naturally to obtain a solid petri dish.
[0034] Preparation Example 3:
[0035] A method for preparing a solid petri dish, specifically including the following process:
[0036] Weigh 9 parts by weight of corn steep liquor dry powder, 15 parts by weight of peptone, and 14 parts by weight of agar and add them to a beaker. Then add 970 parts by weight of purified water, mix and stir. Then place it in a water bath and heat to 55 °C, and stir at a speed of 200 r / min until all the above materials are dissolved. Then take it out and place it in an autoclave, heat up to 121 °C and sterilize for 30 min. After sterilization, pour the medium into a petri dish while it is still hot on a clean bench, and let it cool and solidify naturally to obtain a solid petri dish.
[0037] Preparation Example 4:
[0038] A method for preparing a liquid medium, specifically including the following process:
[0039] Weigh 15 parts by weight of whey powder, 0.8 parts by weight of ammonium sulfate, 1.5 parts by weight of potassium dihydrogen phosphate, and 5 parts by weight of brown sugar and add them to a beaker. Then add 980 parts by weight of purified water, mix and stir. Then place it in a water bath and heat to 50 °C, and stir at a speed of 200 r / min until all the above materials are dissolved. Then take it out and place it in an autoclave, heat up to 121 °C and sterilize for 20 min. After sterilization, let it cool and solidify naturally to obtain a liquid petri dish.
[0040] Preparation Example 5:
[0041] Preparation method of liquid culture medium, specifically including the following process:
[0042] Weigh 17 parts by weight of whey powder, 0.9 part by weight of ammonium sulfate, 1.7 parts by weight of potassium dihydrogen phosphate and 6 parts by weight of brown sugar and add them into a beaker. Then add 990 parts by weight of purified water, mix and stir. Then place it in a water bath and heat to 55 °C, and stir at a speed of 200 r / min until all the above materials are dissolved. Then take it out and place it in an autoclave, heat up to 121 °C and sterilize for 30 min. After sterilization, let it cool naturally and solidify to obtain a liquid culture dish.
[0043] Preparation Example 6:
[0044] Preparation method of liquid culture medium, specifically including the following process:
[0045] Weigh 20 parts by weight of whey powder, 1 part by weight of ammonium sulfate, 2 parts by weight of potassium dihydrogen phosphate and 7 parts by weight of brown sugar and add them into a beaker. Then add 1000 parts by weight of purified water, mix and stir. Then place it in a water bath and heat to 55 °C, and stir at a speed of 200 r / min until all the above materials are dissolved. Then take it out and place it in an autoclave, heat up to 121 °C and sterilize for 30 min. After sterilization, let it cool naturally and solidify to obtain a liquid culture dish.
[0046] Preparation Example 7:
[0047] Preparation method of mixed fermentation broth, specifically including the following process:
[0048] Use a sterilized inoculation loop to gently streak back and forth on the surface of the solid culture dish obtained in Preparation Example 1 with one loop of Monascus purpureus with strain number CICC 40712, and then place it in an incubator at 30 °C for 24 h to obtain a Monascus purpureus spore petri dish; use a sterilized inoculation loop to gently streak back and forth on the surface of the solid culture dish obtained in Preparation Example 1 with one loop of Trichoderma koningii with strain number CICC 13025, and then place it in an incubator at 30 °C for 24 h to obtain a Monascus purpureus spore petri dish; after the cultivation is completed, rinse the Monascus purpureus spore petri dish and the Trichoderma koningii spore petri dish with 20 ml of sterile water respectively, and then combine the rinsing solutions obtained by rinsing to obtain a mixed spore suspension; then, according to 0.005 times the total weight of the liquid culture medium obtained in Preparation Example 4, use a pipette to suck the mixed spore suspension and inoculate it into the liquid culture medium, control the pH range to be 5.0 - 5.5, and then place it in an incubator at 30 °C for 20 h to obtain a mixed fermentation broth, and the colony count measured by the plate counting method is 2.3×10 7 CFU / ml.
[0049] Preparation Example 8:
[0050] Preparation method of mixed fermentation broth, specifically including the following process:
[0051] Using a sterilized inoculation loop, streak a loopful of Monascus purpureus strain numbered CICC 40712 gently back and forth on the surface of the solid culture dish obtained in Preparation Example 2, and then place it in an incubator at 30°C for 24 h to obtain a Monascus purpureus spore petri dish; using a sterilized inoculation loop, streak a loopful of Trichoderma koningii strain numbered CICC 13025 gently back and forth on the surface of the solid culture dish obtained in Preparation Example 2, and then place it in an incubator at 30°C for 24 h to obtain a Monascus purpureus spore petri dish; after the cultivation is completed, rinse the Monascus purpureus spore petri dish and the Trichoderma koningii spore petri dish with 25 ml of sterile water respectively, and then combine the rinsing solutions obtained by rinsing to obtain a mixed spore suspension; then, according to 0.008 times the total weight of the liquid medium obtained in Preparation Example 5, use a pipette to aspirate the mixed spore suspension and inoculate it into the liquid medium, control the pH range at 5.0 - 5.5, and then place it in an incubator at 30°C for 20 h to obtain a mixed fermentation broth, and the colony count measured by the plate counting method is 7.1×10 7 CFU / ml.
[0052] Preparation Example 9:
[0053] A method for preparing a mixed fermentation broth, specifically including the following process:
[0054] Using a sterilized inoculation loop, streak a loopful of Monascus purpureus strain numbered CICC 40712 gently back and forth on the surface of the solid culture dish obtained in Preparation Example 3, and then place it in an incubator at 30°C for 24 h to obtain a Monascus purpureus spore petri dish; using a sterilized inoculation loop, streak a loopful of Trichoderma koningii strain numbered CICC 13025 gently back and forth on the surface of the solid culture dish obtained in Preparation Example 3, and then place it in an incubator at 30°C for 24 h to obtain a Monascus purpureus spore petri dish; after the cultivation is completed, rinse the Monascus purpureus spore petri dish and the Trichoderma koningii spore petri dish with 30 ml of sterile water respectively, and then combine the rinsing solutions obtained by rinsing to obtain a mixed spore suspension; then, according to 0.01 times the total weight of the liquid medium obtained in Preparation Example 6, use a pipette to aspirate the mixed spore suspension and inoculate it into the liquid medium, control the pH range at 5.0 - 5.5, and then place it in an incubator at 30°C for 20 h to obtain a mixed fermentation broth, and the colony count measured by the plate counting method is 1.5×10 8 CFU / ml.
[0055] Example 1:
[0056] A functional organic fertilizer prepared from Rosa roxburghii Tratt residues, specifically including the following process:
[0057] Weigh 2 parts by weight of Rosa roxburghii Tratt residue, 12 parts by weight of traditional Chinese medicine residue (obtained by mixing Eucommia ulmoides residue, Astragalus membranaceus residue and Coptis chinensis residue according to the weight ratio of 1:1:1), and 4 parts by weight of rice straw powder, and mix and blend them evenly to obtain a fermentation base material. According to the weight ratio of the mixed fermentation bacterial liquid to the fermentation base material of 1:80, evenly spray the mixed fermentation bacterial liquid obtained in Preparation Example 7 onto the fermentation base material, and turn the material during the spraying process to enable the mixed bacterial liquid to fully contact the fermentation base material. After the spraying of the mixed fermentation bacterial liquid is completed, spray water into the fermentation base material sprayed with the mixed fermentation bacterial liquid for wet treatment to obtain a substrate to be fermented, and control the water content of the substrate to be fermented to be 55%;
[0058] Subsequently, stack the substrate to be fermented into a windrow with a height of 1 m in a ventilated environment for static composting fermentation (the width can be controlled at about 1.5 m), control the ventilation rate to be 0.4 L / min, turn the pile every 2 days during the fermentation period, and then ferment for 8 days. After the composting fermentation is completed, perform hot air drying treatment to obtain functional organic fertilizer particles.
[0059] Example 2:
[0060] A functional organic fertilizer prepared from Rosa roxburghii Tratt residue specifically includes the following process:
[0061] Weigh 2.5 parts by weight of Rosa roxburghii Tratt residue, 15 parts by weight of traditional Chinese medicine residue (obtained by mixing Eucommia ulmoides residue, Astragalus membranaceus residue and Coptis chinensis residue according to the weight ratio of 1:1:1), and 4.5 parts by weight of rice straw powder, and mix and blend them evenly to obtain a fermentation base material. According to the weight ratio of the mixed fermentation bacterial liquid to the fermentation base material of 1:90, evenly spray the mixed fermentation bacterial liquid obtained in Preparation Example 8 onto the fermentation base material, and turn the material during the spraying process to enable the mixed bacterial liquid to fully contact the fermentation base material. After the spraying of the mixed fermentation bacterial liquid is completed, spray water into the fermentation base material sprayed with the mixed fermentation bacterial liquid for wet treatment to obtain a substrate to be fermented, and control the water content of the substrate to be fermented to be 60%;
[0062] Subsequently, stack the substrate to be fermented into a windrow with a height of 1 m in a ventilated environment for static composting fermentation (the width can be controlled at about 1.5 m), control the ventilation rate to be 0.4 L / min, turn the pile every 2 days during the fermentation period, and then ferment for 9 days. After the composting fermentation is completed, perform hot air drying treatment to obtain functional organic fertilizer particles.
[0063] Example 3:
[0064] A functional organic fertilizer prepared from Rosa roxburghii Tratt residue specifically includes the following process:
[0065] Weigh 3 parts by weight of Rosa roxburghii Tratt residue, 18 parts by weight of traditional Chinese medicine residue (obtained by mixing Eucommia ulmoides residue, Astragalus membranaceus residue and Coptis chinensis residue in a weight ratio of 1:1:1), and 5 parts by weight of rice straw powder, and mix them evenly to obtain a fermentation base material. According to the weight ratio of the mixed fermentation bacterial liquid to the fermentation base material of 1:100, spray the mixed fermentation bacterial liquid obtained in Preparation Example 9 evenly onto the fermentation base material, and turn the material during the spraying process to make the mixed bacterial liquid fully contact the fermentation base material. After the spraying of the mixed fermentation bacterial liquid is completed, spray water into the fermentation base material sprayed with the mixed fermentation bacterial liquid for wet treatment to obtain a substrate to be fermented, and control the water content of the substrate to be fermented to be 65%;
[0066] Subsequently, stack the substrate to be fermented into a windrow with a height of 1.5 m in a ventilated environment for static composting fermentation (the width can be controlled at about 1.5 m), control the ventilation volume to be 0.5 L / min, turn the pile every 2 days during the fermentation period, and then ferment for 10 days. After the composting fermentation is completed, perform hot air drying treatment to obtain functional organic fertilizer particles.
[0067] Comparative Example 1:
[0068] A functional organic fertilizer prepared from Rosa roxburghii Tratt residue specifically includes the following process:
[0069] Weigh 3 parts by weight of Rosa roxburghii Tratt residue, 18 parts by weight of traditional Chinese medicine residue (obtained by mixing Eucommia ulmoides residue, Astragalus membranaceus residue and Coptis chinensis residue in a weight ratio of 1:1:1), and 5 parts by weight of rice straw powder, and mix them evenly to obtain a fermentation base material. According to the weight ratio of the mixed fermentation bacterial liquid to the fermentation base material of 1:100, spray the mixed fermentation bacterial liquid obtained in Preparation Example 9 evenly onto the fermentation base material, and turn the material during the spraying process to make the mixed bacterial liquid fully contact the fermentation base material. After the spraying of the mixed fermentation bacterial liquid is completed, spray water into the fermentation base material sprayed with the mixed fermentation bacterial liquid for wet treatment to obtain a substrate to be fermented, and control the water content of the substrate to be fermented to be 40%;
[0070] Subsequently, stack the substrate to be fermented into a windrow with a height of 1.5 m in a ventilated environment for static composting fermentation (the width can be controlled at about 1.5 m), control the ventilation volume to be 0.5 L / min, turn the pile every 2 days during the fermentation period, and then ferment for 10 days. After the composting fermentation is completed, perform hot air drying treatment to obtain functional organic fertilizer particles.
[0071] Comparative Example 2:
[0072] A functional organic fertilizer prepared from Rosa roxburghii Tratt residue specifically includes the following process:
[0073] Weigh 3 parts by weight of Rosa roxburghii Tratt residue, 18 parts by weight of traditional Chinese medicine residue (obtained by mixing Eucommia ulmoides residue, Astragalus membranaceus residue and Coptis chinensis residue according to the weight ratio of 1:1:1), and 5 parts by weight of rice straw powder, and mix and blend them evenly to obtain a fermentation substrate. According to the weight ratio of the mixed fermentation bacterial liquid to the fermentation substrate of 1:100, evenly spray the mixed fermentation bacterial liquid obtained in Preparation Example 9 onto the fermentation substrate, and turn the material during the spraying process to make the mixed bacterial liquid fully contact the fermentation substrate. After the spraying of the mixed fermentation bacterial liquid is completed, spray water into the fermentation substrate sprayed with the mixed fermentation bacterial liquid for wetting treatment to obtain a substrate to be fermented, and control the water content of the substrate to be fermented to be 65%;
[0074] Subsequently, stack the substrate to be fermented into a strip stack with a height of 1.5 m in a ventilated environment for static composting fermentation (the width can be controlled at about 1.5 m), control the ventilation volume to be 0.1 L / min, turn the pile every 2 days during the fermentation period, and then ferment for 10 days. After the composting fermentation is completed, perform hot air drying treatment to obtain functional organic fertilizer particles.
[0075] Comparative Example 3:
[0076] A functional organic fertilizer prepared from Rosa roxburghii Tratt residue specifically includes the following process:
[0077] Weigh 3 parts by weight of Rosa roxburghii Tratt residue, 18 parts by weight of traditional Chinese medicine residue (obtained by mixing Eucommia ulmoides residue, Astragalus membranaceus residue and Coptis chinensis residue according to the weight ratio of 1:1:1), and 5 parts by weight of rice straw powder, and mix and blend them evenly to obtain a fermentation substrate. According to the weight ratio of the mixed fermentation bacterial liquid to the fermentation substrate of 1:100, evenly spray the mixed fermentation bacterial liquid obtained in Preparation Example 9 onto the fermentation substrate, and turn the material during the spraying process to make the mixed bacterial liquid fully contact the fermentation substrate. After the spraying of the mixed fermentation bacterial liquid is completed, spray water into the fermentation substrate sprayed with the mixed fermentation bacterial liquid for wetting treatment to obtain a substrate to be fermented, and control the water content of the substrate to be fermented to be 65%;
[0078] Subsequently, stack the substrate to be fermented into a strip stack with a height of 1.5 m in a ventilated environment for static composting fermentation (the width can be controlled at about 1.5 m), control the ventilation volume to be 0.5 L / min, turn the pile every 2 days during the fermentation period, and then ferment for 12 days. After the composting fermentation is completed, perform hot air drying treatment to obtain functional organic fertilizer particles.
[0079] Effect test after crop fertilization:
[0080] Take a plastic pot with a length of 500 mm, a width of 200 mm and a height of 120 mm, mix and blend evenly according to the weight ratio of soil to functional organic fertilizer particles of 30:1 and fill it into the plastic pot, and then cultivate lettuce seeds in the plastic pot and water to moisten the soil. Then harvest on the 80th day and measure the chlorophyll content with a handheld chlorophyll fluorometer. The results are shown in Table 1 below.
[0081] Table 1
[0082] Material source Chlorophyll content (SPAD) Example 1 23.2 Example 2 25.1 Example 3 25.8 Comparative Example 1 14.2 Comparative Example 2 19.5 Comparative Example 3 17.6
[0083] From the above Table 1, the following conclusions can be drawn:
[0084] (1) Through Examples 1 to 3, it can be found that the present invention uses Rosa roxburghii Tratt residue, traditional Chinese medicine residue and rice husk powder as the fermentation base material for preparing the functional organic fertilizer. Through mixing in a specific ratio, and then spraying the constructed mixed fermentation bacterial liquid on the fermentation base material, the functional organic fertilizer is obtained through composting fermentation. When used for fertilizing crops, it can promote the growth of crops.
[0085] (2) Through Comparative Example 1, it can be found that due to reducing the water content of the substrate to be fermented before fermentation, it may lead to incomplete fermentation in this fermentation system, and further lead to poor fertilizer efficiency of the functional organic fertilizer and poor performance in promoting crop growth.
[0086] (3) Through Comparative Example 2, it can be found that due to reducing the ventilation volume during fermentation, it may lead to limited oxygen supply in this fermentation system, poor aerobic fermentation process of the mixed fermentation bacterial liquid, inability to fully decompose organic matter, resulting in a slow fermentation rate and incomplete fermentation, and further leading to poor fertilizer efficiency of the functional organic fertilizer and poor performance in promoting crop growth.
[0087] (4) Through Comparative Example 3, it can be found that due to extending the fermentation time, it may cause adverse effects such as nutrient loss, imbalance of nutrient ratio and decline of fermentation bacteria activity in this fermentation system, and further lead to poor fertilizer efficiency of the functional organic fertilizer and poor performance in promoting crop growth.
[0088] The above-described embodiments have detailed the technical solutions and beneficial effects of the present invention. It should be understood that the above are only specific embodiments of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed.
Claims
1. A functional organic fertilizer prepared from Rosa roxburghii Tratt residue, characterized in that, The preparation method of the functional organic fertilizer comprises the following steps: The rosa roxburghii tratt residue, traditional Chinese medicine residue and rice straw powder are mixed to form a fermentation base material, and the mixed fermentation bacterial liquid is sprayed onto the fermentation base material and then wetted with water to form a to-be-fermented base material; The fermentation base material is successively subjected to compost fermentation and drying treatment to obtain the functional organic fertilizer.
2. The functional organic fertilizer prepared from Rosa roxburghii Tratt residue according to claim 1, wherein, The weight ratio of the rosa roxburghii tratt residue: traditional Chinese medicine residue: rice straw powder is 2-3:12-18:4-5.
3. The functional organic fertilizer prepared from rosa roxburghii tratt residue according to claim 1, wherein, The preparation method of the mixed fermentation bacterial liquid comprises the following steps: Monascus purpureus and Trichoderma koningii are respectively inoculated into a solid culture dish and placed in an incubator at 30 °C for 24 h to obtain a Monascus purpureus spore culture dish and a Trichoderma koningii spore culture dish; The Monascus purpureus spore culture dish and the Trichoderma koningii spore culture dish are rinsed with sterile water and the rinsing solutions are combined to obtain a mixed spore suspension; The mixed spore suspension is inoculated into a liquid culture medium for culture to obtain a mixed fermentation bacterial liquid.
4. The functional organic fertilizer prepared from Rosa roxburghii Tratt residue according to claim 3, characterized in that, The strain number of the Monascus purpureus is CICC 40712.
5. The functional organic fertilizer prepared from Rosa roxburghii Tratt residue according to claim 3, wherein The strain number of the Trichoderma koningii is CICC 13025.
6. The functional organic fertilizer prepared from Rosa roxburghii Tratt residue according to claim 3, wherein The inoculation amount of the inoculated mixed spore suspension is 0.005-0.01 of the total weight of the liquid culture medium.
7. The functional organic fertilizer prepared from Rosa roxburghii tratt residue according to claim 1, wherein, The weight ratio of the mixed fermentation bacterial liquid: fermentation base material is 1:80-100.
8. The functional organic fertilizer prepared from Rosa roxburghii Tratt residue according to claim 1, wherein, The water content of the to-be-fermented base material is 55%-65%.
9. The functional organic fertilizer prepared from Rosa roxburghii Tratt residue according to claim 1, wherein The conditions of the compost fermentation include that the compost height is 1 m-1.5 m, the fermentation time is 8 days-10 days, the turning frequency is once every 2 days, and the ventilation rate is 0.4 L / min-0.5 L / min.