Chicken protein type amino acid water-soluble fertilizer

Abstract

The invention belongs to the field of fertilizers, and particularly relates to a chicken protein type amino acid water-soluble fertilizer. The water-soluble fertilizer comprises the following components in parts by weight: 40-60 parts of chicken protein enzymatic hydrolysate, 1-10 parts of potassium fulvate and 1-10 parts of chitosan oligosaccharide, the preparation method of the chicken protein enzymatic hydrolysate comprises the following steps: mincing chicken leftovers into meat paste, adding deionized water according to a certain solid-to-liquid ratio, carrying out ultrasonic pretreatment, adding protease, carrying out enzymolysis for a certain time, carrying out enzyme deactivation, and carrying out centrifugal filtration to obtain a supernatant (enzymatic hydrolysate). The chicken protein type amino acid water-soluble fertilizer prepared by the invention can be used for improving crop growth traits and comprehensively improving crop growth indexes and quality indexes.

Description

Technical Field

[0001] The present invention belongs to fertilizers, and particularly relates to a chicken protein type amino acid water-soluble fertilizer. Background Art

[0002] With the rapid development of modern agriculture, consumers' demand for high-quality fruit and vegetable products continues to climb, prompting growers to over-rely on chemical fertilizers to increase output. Although this production mode improves crop yields in the short term, in the long run, it has caused a series of ecological problems: problems such as soil structure damage, microbial community imbalance, and nutrient utilization rate decline have become increasingly prominent. At the same time, the excessive use of chemical fertilizers may also lead to heavy metal accumulation and nitrate pollution, posing a double threat to agricultural product safety and the ecological environment.

[0003] Under this background, actively promoting the transformation of green fertilization technology and developing new and efficient organic fertilizers have become important topics for the current sustainable development of agriculture. An ideal organic fertilizer should have characteristics such as controllable nutrient release, low pathogen content, good environmental compatibility, etc., and at the same time be able to improve the soil microecological environment. Organic water-soluble fertilizer is a new type of fertilizer that can dissolve in water. It is a multi-component compound fertilizer that can be completely dissolved in water and can be quickly dissolved in water, thus achieving many advantages such as easy absorption and quick effect. The main application methods are 3 types: drip irrigation application in the field, spraying on the plant leaves, and applying as a potted plant nutrient solution. The main advantages are the improvement of nutrient absorption rate and the ability to regulate the fertilization concentration. Summary of the Invention

[0004] In order to achieve the above object, the technical solution adopted by the present invention is to provide a chicken protein type amino acid water-soluble fertilizer. The specific technical solution is as follows: A chicken protein type amino acid water-soluble fertilizer contains the following components in parts by weight: 40 - 60 parts of chicken protein hydrolyzate, 1 - 10 parts of potassium humate, and 1 - 10 parts of chitosan oligosaccharide; The preparation method of the chicken protein hydrolyzate is: after crushing chicken offal into minced meat, adding deionized water according to a certain solid-liquid ratio for ultrasonic pretreatment, adding protease for enzymatic hydrolysis for a certain time, then inactivating the enzyme, and then centrifuging and filtering to obtain the supernatant (hydrolyzate); The solid-liquid ratio is 1∶3 - 5 g / mL; The ultrasonic treatment conditions are: 400 - 600 W, 25 - 35 min; The enzymatic hydrolysis time is: 3 - 5 h; The protease addition amount is: 0.15 - 0.25%; The protease is composed of alkaline protease, flavor protease, and pepsin according to a mass ratio of 1 - 3∶1 - 3∶1 - 3.

[0005] Preferably, the chicken protein type amino acid water-soluble fertilizer comprises the following components in parts by weight: 50 parts of chicken protein hydrolysate, 5 parts of potassium humate, and 5 parts of chitosan oligosaccharide.

[0006] Preferably, for the chicken protein type amino acid water-soluble fertilizer, the solid-liquid ratio is 1:4 g / mL; the ultrasonic treatment conditions are: 500 W, 30 min; the enzymolysis time is 4 h; the protease addition amount is 0.2%; Preferably, the protease is composed of alkaline protease, flavor protease, and pepsin in a mass ratio of 1:1:1.

[0007] The use of the chicken protein type amino acid water-soluble fertilizer prepared by the present invention for improving the growth traits of crops. The crops are tomatoes or Chinese cabbages.

[0008] Compared with the prior art, the beneficial effects of the present invention are: The chicken protein type amino acid water-soluble fertilizer can make full use of meat food processing waste, is green and environmentally friendly, and can effectively improve problems such as soil environmental deterioration and food safety threats caused by long-term application of chemical fertilizers. It provides a reference basis for the scientific and reasonable application of water-soluble fertilizers in vegetables.

[0009] The chicken protein type amino acid water-soluble fertilizer prepared by the present invention can significantly improve the growth indexes and quality indexes of crops. Specific Embodiments

[0010] To further understand the present invention, the present invention will be described in detail below in conjunction with embodiments.

[0011] Example 1: Preparation of Chicken Protein Type Amino Acid Water-Soluble Fertilizer 1.1 Preparation of chicken protein hydrolysate: Enzymolysis solution A After the chicken offal is crushed into minced meat, deionized water is added according to an appropriate solid-liquid ratio for ultrasonic pretreatment, protease is added for enzymolysis for a certain time, then the enzyme is inactivated, and then centrifuged and filtered to obtain the supernatant (enzymolysis solution) for standby; The solid-liquid ratio is 1:4 (g / mL); The ultrasonic treatment conditions are: 500 W, 30 min; The centrifugation conditions are: 4 °C, 5000 r / min, 20 min; The enzymolysis time is 4 h The protease addition amount is 0.2%.

[0012] The protease is composed of alkaline protease, flavor protease, and pepsin in a mass ratio of 1:1:1; Enzymolysis solution B: The protease is composed of alkaline protease and flavor protease in a mass ratio of 1:1; the remaining methods are the same as those of enzymolysis solution A.

[0013] Enzyme hydrolysate C: Protease: composed of alkaline protease and pepsin in a mass ratio of 1:1; the remaining methods are the same as those of enzyme hydrolysate A.

[0014] Enzyme hydrolysate D: Protease: composed of flavor protease and pepsin in a mass ratio of 1:1; the remaining methods are the same as those of enzyme hydrolysate A.

[0015] Enzyme hydrolysate E: Protease: composed of alkaline protease, flavor protease, pepsin and trypsin in a mass ratio of 1:1:1:1; the remaining methods are the same as those of enzyme hydrolysate A.

[0016] Enzyme hydrolysate F: Using fish scraps to replace fish scraps, the remaining methods are the same as those of enzyme hydrolysate A: Note: Source of protease: Shandong Gukang Bioengineering Co., Ltd., food grade.

[0017] 1.2 Formulation composition Table 1: Formula of chicken protein type amino acid water-soluble fertilizer (parts by weight) ; Example 2: Application of water-soluble fertilizer on tomatoes Test method: Conduct tests in a greenhouse. The tomato planting density is 2,600 plants per mu. A total of 6 treatments are set, with 1 blank. Each treatment has 4 replicates, randomly arranged. The plot area is 35m 2 . The nitrogen, phosphorus and potassium contents of each treatment are kept consistent. Different ratios of water-soluble fertilizers are topdressed twice at intervals of 20 days, with a dosage of 300 g / mu / time calculated based on potassium humate, and tap water topdressing is used as a blank control (CK). After the first cluster of fruits is harvested, the fruit quality and plant trait indicators are measured.

[0018] Index test: Determination of growth index: Randomly select 10 plants from each plot and measure the stem diameter using a vernier caliper. Randomly pick 10 mature fruits from each plot, weigh them separately with an electronic scale, and take the average value.

[0019] Determination of quality index: Soluble solids are determined using a handheld digital refractometer, total sugar content is determined using the Fehling reagent method, total acidity is determined according to the national standard GB / T 12456-2008 for the determination of total acid in foods, and the sugar-acid ratio is calculated. Vc content is determined using the 2,6-dichlorophenol indophenol titration method.

[0020] Results and analysis: Table 2 Effects of different treatments on the growth and development of tomatoes ; As can be seen from Table 2, in terms of the stem diameter of tomatoes, compared with CK, the stem diameters of T1 - T6 treatments all increased significantly, and there were significant differences between each treatment and CK; in terms of tomato yield, compared with CK, T1 - T6 treatments had significant increases in yield respectively, and there were significant differences between each treatment and CK. Compared T1 with T6, and compared with fish meat, using chicken protein type amino acid water-soluble fertilizer had better effects; compared T1 with T2 - T5, the composition of protease would have a relatively large impact on the fertilizer efficiency of water-soluble fertilizers. It was preferred that the protease was composed of alkaline protease, flavor protease and pepsin according to the mass ratio of 1:1:1.

[0021] Table 3 Effects of Different Treatments on Tomato Quality ; As can be seen from Table 3, in terms of the stem diameter of tomatoes, compared with CK, the soluble solids of T1 - T6 treatments all increased significantly, and there were significant differences between each treatment and CK; in terms of the sugar-acid ratio of tomatoes, compared with CK, T1 - T6 treatments increased significantly respectively, and there were significant differences between each treatment and CK. In terms of the Vc content of tomatoes, compared with CK, T1 - T6 treatments increased significantly respectively, and there were significant differences between each treatment and CK. Compared T1 with T6, and compared with fish meat, using chicken protein type amino acid water-soluble fertilizer had better effects; compared T1 with T2 - T5, the composition of protease would have a relatively large impact on the fertilizer efficiency of water-soluble fertilizers. It was preferred that the protease was composed of alkaline protease, flavor protease and pepsin according to the mass ratio of 1:1:1.

[0022] Example 3: Application of Water-Soluble Fertilizer on Chinese Cabbage Test method: The test was carried out in a potting method. 2 plants were planted in each pot. A randomized block experiment was used with 6 treatments. 15 days after sowing, the Chinese cabbage was first foliar sprayed with water-soluble fertilizer, and the second foliar spraying was carried out at an interval of 7 days. The spraying concentration was 180 mg / L based on potassium humate. Each treatment was repeated 3 times, and 10 pots were set for each repetition. Spraying with tap water was used as the blank control (CK). After 30 days, random sampling was carried out in each plot to measure the sample plant indexes.

[0023] Index test: Growth index determination: Measure the plant height and fresh weight morphological indexes of the sample plants Table 4 Effects of Different Treatments on the Growth and Development of Chinese Cabbage ; As can be seen from Table 4, in terms of the plant height of Chinese cabbage, compared with CK, the plant heights of the T1 - T6 treatments were all significantly increased, and there were significant differences between each treatment and CK; in terms of the fresh weight of Chinese cabbage, compared with CK, the T1 - T6 treatments were significantly increased respectively, and there were significant differences between each treatment and CK. Compared with T6, compared with fish meat, the effect of using chicken protein amino acid water-soluble fertilizer is better; compared with T2 - T5, the composition of protease will have a relatively large impact on the fertilizer efficiency of water-soluble fertilizer. It is preferred that the protease is composed of alkaline protease, flavor protease and pepsin in a mass ratio of 1:1:1.

[0024] The embodiments described above are only descriptions of the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A water-soluble fertilizer of chicken protein type amino acids, characterized in that, It contains the following components in parts by weight: 40-60 parts of chicken protein hydrolysate, 1-10 parts of potassium fulvate, and 1-10 parts of chitosan oligosaccharide; The preparation method of the chicken protein hydrolysate is as follows: After the chicken scraps are crushed into minced meat, deionized water is added according to a certain solid-liquid ratio for ultrasonic pretreatment. After adding protease for enzymatic hydrolysis for a certain time, the enzyme is inactivated, and then centrifuged and filtered to obtain the supernatant (hydrolysate); The solid-liquid ratio is 1∶3-5 g / mL; Ultrasonic treatment conditions: 400-600 W, 25-35 min; Enzymatic hydrolysis time: 3-5 h; Protease addition amount: 0.15-0.25%; Protease: It is composed of alkaline protease, flavor protease, and pepsin in a mass ratio of 1-3:1-3:1-3.

2. The water-soluble fertilizer of chicken protein type amino acid according to claim 1, characterized in that It contains the following components in parts by weight: 50 parts of chicken protein hydrolysate, 5 parts of potassium fulvate, and 5 parts of chitosan oligosaccharide.

3. The water-soluble amino acid fertilizer of chicken protein type according to claim 1 or 2, characterized in that The solid-liquid ratio is 1∶4 g / mL; Ultrasonic treatment conditions: 500 W, 30 min; Enzymatic hydrolysis time: 4 h; Protease addition amount: 0.2%; Protease: It is composed of alkaline protease, flavor protease, and pepsin in a mass ratio of 1:1:

1.

4. Use of the water-soluble amino acid fertilizer of chicken protein type according to any one of claims 1-3 for improving the growth traits of crops.

5. The use according to claim 4, characterized in that, The said ones are tomatoes or Chinese cabbages.