Bletilla striata antibacterial skin-care traditional Chinese medicine cold-processed soap and preparation method thereof

Abstract

The invention discloses bletilla striata antibacterial skin-care traditional Chinese medicine cold soap and a preparation method thereof, and belongs to the technical field of washing products. The cold soap is prepared from 1-4 parts by mass of bletilla striata extract, 6-15 parts by mass of olive oil, 2-7 parts by mass of coconut oil, 2-7 parts by mass of palm oil, 2-4 parts by mass of sodium hydroxide, 4-6 parts by mass of ultrapure water and 4 parts by mass of essential oil. Wherein the bletilla striata extract is obtained through the processes of ethanol soaking and solvent step-by-step extraction, and natural bacteriostatic active ingredients are reserved. According to the preparation method of the bletilla striata antibacterial skin-care traditional Chinese medicine cold soap, a cold soap process is adopted, after mixed grease and alkali liquor are saponified at the temperature of 40-50 DEG C, an extract and essential oil are added, and the mixture is put into a mold to be cooked to form a soap body. The pH value of the cold soap product is 6.0-8.0, escherichia coli, staphylococcus aureus and candida albicans can be remarkably inhibited, the diameter of an inhibition zone reaches 12-24 mm, and the cleaning power is superior to that of commercially available products. Meanwhile, the foam is rich, the cold and heat stability is high (moisture volatile matter 1t; 7.2%), and the foam is mild and skin-friendly.

Description

Technical Field

[0001] The invention relates to the technical field of washing products, and in particular to a bletilla striata antibacterial and skin-care Chinese medicine cold-processed soap and a preparation method thereof. Background Art

[0002] As a basic cleaning product, soap can be traced back to the Mesopotamian civilization in 3000 BC. In the early days, it was prepared by mixing animal fat and wood ash. With the development of industrialization, modern soaps mostly use chemical synthetic surfactants (such as sodium dodecyl sulfate), synthetic pigments and preservatives to meet the needs of large-scale production. Although this type of chemical synthetic soap has the characteristics of strong detergency and low cost, long-term use can easily lead to damage to the skin barrier function, causing problems such as clogged pores, dryness and allergies, and even accelerate skin aging. In addition, residual ingredients such as alkylbenzene sulfonates in synthetic detergents are difficult to degrade and may cause cumulative pollution to the aquatic ecological environment.

[0003] In recent years, natural soaps have gradually attracted attention in order to reduce the negative impact of chemical synthetic ingredients on the skin and the environment. In the prior art, there are solutions that use plant extracts (such as tea tree oil and aloe vera) to replace some chemical antibacterial agents. However, such products still have the following limitations:

[0004] 1. Insufficient antibacterial effect: Most natural extracts have low active ingredient content, and traditional hot soap making processes (such as high-temperature saponification) easily destroy heat-sensitive antibacterial substances, resulting in limited inhibitory effects on common pathogens (such as Escherichia coli and Staphylococcus aureus);

[0005] 2. It is difficult to balance mildness and cleaning power: when the proportion of natural oil is too high, it can improve moisturizing properties, but it will significantly reduce the richness of foam and cleaning efficiency;

[0006] 3. Stability defects: Some cold-process soaps are prone to problems such as soap softening and active ingredient volatilization due to improper moisture control or imperfect aging process, and have a short storage period.

[0007] In response to the above problems, the industry has tried to improve the performance by optimizing the extraction process (such as supercritical CO2 extraction) or compounding antibacterial agents, but the former is costly and the latter is still difficult to completely get rid of the use of chemical additives. Therefore, developing a natural cold-processed soap that has both high antibacterial properties, mild skin-friendliness and environmental friendliness, and overcoming the technical bottleneck of the low retention rate of active ingredients in the existing preparation process has become a problem that needs to be solved in the field of detergents. Summary of the invention

[0008] To solve the problems caused by the long-term use of chemically synthesized surfactants, such as damaged skin barrier function, pore blockage, dryness and allergies, and even accelerated skin aging, as well as the insufficient antibacterial effect, the difficulty in balancing mildness and detergency, and the stability defects faced by plant extracts, the present invention provides a mild, highly antibacterial and stable Bletilla striata antibacterial skin care traditional Chinese medicine cold-processed soap and its preparation method.

[0009] To achieve the above object, the technical scheme adopted by the present invention is as follows:

[0010] The formula of the Bletilla striata antibacterial skin care traditional Chinese medicine cold-processed soap provided by the present invention is: 1-4 parts by mass of Bletilla striata extract, 6-15 parts by mass of olive oil, 2-7 parts by mass of coconut oil, 2-7 parts by mass of palm oil, 2-4 parts by mass of sodium hydroxide, 4-6 parts by mass of ultrapure water, and 4 parts by mass of essential oil.

[0011] The essential oil is one or more of borneol essential oil, citrus essential oil, and sweet orange essential oil.

[0012] The Bletilla striata extract includes one or more of Bletilla striata petroleum ether extract, Bletilla striata dichloromethane extract, Bletilla striata ethyl acetate extract, and Bletilla striata n-butanol extract.

[0013] Preferably, the Bletilla striata extract is Bletilla striata ethyl acetate extract.

[0014] According to the above technical features, the preparation method of the Bletilla striata extract is as follows:

[0015] Crush and sieve the Bletilla striata tubers, soak them in 80% ethanol for 5-7 days, and collect the ethanol extract; use reduced pressure rotary evaporation to remove ethanol to obtain the total extract, dissolve the total extract in ultrapure water, and extract with petroleum ether, dichloromethane, ethyl acetate, and n-butanol respectively, collect the extracts, and rotary evaporate to dryness; obtain Bletilla striata petroleum ether extract, Bletilla striata dichloromethane extract, Bletilla striata ethyl acetate extract, and n-butanol extract.

[0016] Preferably, the weight mass fractions of the Bletilla striata extract are 1 part by mass, 2 parts by mass, 3 parts by mass, and 4 parts by mass respectively.

[0017] According to the above technical features, the preparation method of the Bletilla striata antibacterial skin care traditional Chinese medicine cold-processed soap is:

[0018] Weigh olive oil, coconut oil, and palm oil according to parts by weight, mix and stir well to obtain a mixed oil and fat; weigh sodium hydroxide and ultrapure water according to parts by weight. Under the condition of a water bath, add sodium hydroxide to ultrapure water and stir until completely dissolved to obtain an alkali solution; preheat the mixed oil and fat and the alkali solution to 40 - 50 °C, slowly pour the alkali solution into the mixed oil and fat, keep the temperature at 40 - 50 °C, use a stirrer to stir evenly at a constant speed for 2 - 4 h to saponify it; weigh the extract of Bletilla striata according to parts by weight, put it into the above saponification solution and continue to stir evenly; weigh the essential oil according to parts by weight, put it into the soap solution and continue to stir evenly. When the soap solution can draw an "8" shape and does not disappear within 5 - 10 seconds, it can be put into the mold; after putting it into the mold, demold and cure it to obtain the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap.

[0019] After putting it into the mold, keep it warm for 24 hours, demold it after 2 - 3 days, and cure it in an incubator protected from light with constant temperature and humidity for at least 1 month.

[0020] The temperature of the incubator is maintained at 28 °C - 30 °C, and the humidity is kept at 40% - 50%.

[0021] According to the above technical features, the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap can inhibit the growth of Escherichia coli, Candida albicans, and Staphylococcus aureus.

[0022] According to the above technical features, the pH of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap is 6.0 - 8.0.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] 1. The Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the present invention can reduce the skin damage caused by many commercially available soaps. It has natural ingredients, and the natural glycerol obtained through saponification reaction. At the same time, the proportion of olive oil is relatively large, which is very friendly to dry skin and can play a good moisturizing role on the skin. The extract of the Chinese herbal medicine Bletilla striata uses this natural antibacterial agent and shows excellent antibacterial effects compared with many commercially available soaps. It can reduce the occurrence of skin diseases. While protecting one's own health, it can also have a mild, natural, and non-irritating experience and is suitable for long-term use.

[0025] 2. The Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the present invention has a smooth and moist soap body, can preserve its nutritional components to the greatest extent, has dense and rich foam, and is mild and skin-friendly.

[0026] 3. The pH value of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the present invention is close to neutral, greatly reducing the irritation and being suitable for sensitive skin. Description of the Drawings

[0027] Figure 1The Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the present invention.

[0028] Figure 2 It is a chart of the antibacterial zone diameters of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the present invention and a commercially available soap. The 0, 1, 2, 3, 4 on the horizontal axis represent the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soaps containing 0, 1, 2, 3, 4 g of ethyl acetate extract.

[0029] Figure 3 It is an antibacterial diagram of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the present invention and a commercially available soap. 0, 1, 2, 3, 4 respectively represent the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soaps containing 0, 1, 2, 3, 4 g of ethyl acetate extract.

[0030] Figure 4 It is a chart of the foaming power of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the invention. The 0, 1, 2, 3, 4 on the horizontal axis respectively represent the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soaps containing 0, 1, 2, 3, 4 g of ethyl acetate extract.

[0031] Figure 5 It is a test comparison chart of the cleanliness of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the present invention and a commercially available soap. Detailed implementation mode

[0032] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work conditions belong to the scope of protection of the present invention.

[0033] Example 1

[0034] The specific preparation steps of the Bletilla striata extract are as follows:

[0035] S1. Weigh dry Bletilla striata tubers, crush them with a traditional Chinese medicine grinder, and sieve them through a 50-mesh sieve. Soak the Bletilla striata tuber powder with 80% ethanol at a material-liquid ratio of 1:5 (g / mL), soak for 5 - 7 days each time, stir once a day to make it fully mixed. Soak 3 times, collect and combine the ethanol extracts of the 3 times, and concentrate them under reduced pressure to a total flow extract at 55°C, and dry them in a vacuum box at 50°C for 24 - 48 h.

[0036] S2. Take the total flow extract, dissolve it with pure water to make it evenly dispersed, and the material-liquid ratio is 1:3 (g:mL). Extract 3 - 5 times successively with equal volumes of petroleum ether, dichloromethane, ethyl acetate, and n-butanol. Combine the corresponding extracts and concentrate them under reduced pressure to obtain the extracts of the corresponding extraction phases.

[0037] The specific preparation steps of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap are as follows:

[0038] S1. Weigh 6.7 parts by weight of olive oil, 6.7 parts by weight of coconut oil, and 6.7 parts by weight of palm oil, mix and stir evenly to obtain mixed oils and fats.

[0039] S2. Weigh 3.1 parts by weight of sodium hydroxide and 5.27 parts by weight of ultrapure water. Under the condition of a cold water bath, add sodium hydroxide to ultrapure water and stir until completely dissolved to obtain an alkali solution.

[0040] S3. After preheating the mixed oils and fats and the alkali solution to the same temperature (42 °C), slowly pour the alkali solution into the mixed oils and fats, keep the temperature at 42 °C, and use a stirrer to stir evenly at a constant speed for 7 h to saponify it.

[0041] S4. Weigh 4 parts by weight of ethyl acetate extract, put it into S4 and continue to stir evenly.

[0042] S5. Weigh 4 parts by weight of borneol essential oil, put it into the soap solution, continue to stir evenly. When the soap solution can be stirred to draw an "8" shape and does not disappear within a few seconds, it can be put into the mold.

[0043] S6. After putting it into the mold, demold and cure it to obtain the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap.

[0044] Example 2

[0045] The specific preparation steps of the Bletilla striata extract are the same as those in Example 1.

[0046] The specific preparation steps of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap are as follows:

[0047] S1. Weigh 14.3 parts by weight of olive oil, 2.9 parts by weight of coconut oil, and 2.9 parts by weight of palm oil, mix and stir evenly to obtain mixed oils and fats.

[0048] S2. Weigh 2.85 parts by weight of sodium hydroxide and 4.85 parts by weight of ultrapure water. Under the condition of a cold water bath, add sodium hydroxide to ultrapure water and stir until completely dissolved to obtain an alkali solution.

[0049] S3. After preheating the mixed oils and fats and the alkali solution to the same temperature (42 °C), slowly pour the alkali solution into the mixed oils and fats, keep the temperature at 42 °C, and use a stirrer to stir evenly at a constant speed for 6 h to saponify it.

[0050] S4. Weigh 2 parts by weight of ethyl acetate extract, put it into S4 and continue to stir evenly.

[0051] S5. Weigh 4 parts by weight of citrus essential oil, put it into the soap solution, and continue to stir evenly. When the soap solution can be drawn into an "8" shape and does not disappear within a few seconds, it can be poured into the mold.

[0052] S6. After pouring into the mold, demold and cure to obtain the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap.

[0053] Example 3

[0054] The specific preparation steps of the Bletilla striata extract are the same as those in Example 1.

[0055] The specific preparation steps of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap are as follows:

[0056] S1. Weigh 12 parts by weight of olive oil, 4 parts by weight of coconut oil, and 4 parts by weight of palm oil, mix and stir evenly to obtain a mixed oil.

[0057] S2. Weigh 2.93 parts by weight of sodium hydroxide and 4.98 parts by weight of ultrapure water. Under the condition of a cold water bath, add sodium hydroxide to ultrapure water and stir until completely dissolved to obtain an alkali solution.

[0058] S3. After preheating the mixed oil and the alkali solution to the same temperature (42°C), slowly pour the alkali solution into the mixed oil, keep the temperature at 42°C, and use a stirrer to stir evenly for 6 hours to saponify it.

[0059] S4. Weigh 3 parts by weight of ethyl acetate extract, and put it into S4 and continue to stir evenly.

[0060] S5. Weigh 4 parts by weight of sweet orange essential oil, put it into the soap solution, and continue to stir evenly. When the soap solution can be drawn into an "8" shape and does not disappear within a few seconds, it can be poured into the mold.

[0061] S6. After pouring into the mold, demold and cure to obtain the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap.

[0062] Example 4

[0063] The specific preparation steps of the Bletilla striata extract are the same as those in Example 1.

[0064] The specific preparation steps of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap are as follows:

[0065] S1. Weigh 10 parts by weight of olive oil, 4 parts by weight of coconut oil, and 6 parts by weight of palm oil, mix and stir evenly to obtain a mixed oil.

[0066] S2. Weigh 3.26 parts by weight of sodium hydroxide and 5.54 parts by weight of ultrapure water. Under the condition of a cold water bath, add sodium hydroxide to ultrapure water and stir until completely dissolved to obtain an alkaline solution.

[0067] S3. After preheating the mixed oil and fat and the alkaline solution to the same temperature (42 °C), slowly pour the alkaline solution into the mixed oil and fat, keep the temperature at 42 °C, and use a stirrer to stir evenly at a constant speed for 4 h to saponify it.

[0068] S4. Weigh 1 part by weight of ethyl acetate extract and put it into S4 and continue to stir evenly.

[0069] S5. Weigh 2 parts by weight of sweet orange essential oil and 2 parts by weight of borneol essential oil, put them into the soap solution, and continue to stir evenly. When the soap solution can draw an "8" shape and does not disappear within a few seconds, it can be put into the mold.

[0070] S6. After putting it into the mold, demold and cure it to obtain the Bletilla striata antibacterial skin care traditional Chinese medicine cold-processed soap.

[0071] Performance Test

[0072] 1. Antibacterial Test

[0073] Prepare the culture medium:

[0074] Weigh 4 g of tryptone, 2 g of yeast powder, 4 g of sodium chloride, and 6 g of agar into a conical flask, add 400 mL of ultrapure water, sterilize it with a high-temperature and high-pressure steam sterilizer (121 °C, 20 min), place it on a sterile operating table, and set it aside after dispensing.

[0075] Activation of the strain and preparation of the bacterial suspension:

[0076] Activation of the strain: Pick a well-preserved single colony from the solid culture medium onto a new solid culture medium and activate it in an incubator at 37 °C for 24 h.

[0077] Preparation of the bacterial suspension: Use an inoculation loop to pick a part of the colony on the solid culture medium and put it into 20 mL of sterilized 0.85% physiological saline. Measure the absorbance value at 600 nm with an ultraviolet spectrophotometer and dilute it with sterilized 0.85% physiological saline to an absorbance of 0.096 Abs. At this time, the bacterial concentration of the bacterial solution is 1x10^8 CFU / mL.

[0078] Determination of the inhibition zone:

[0079] The size of the antibacterial circle of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap was measured by the punching method. Pour about 50 °C sterilized LB liquid culture medium into a petri dish, place it flat, and wait for it to cool and solidify. Dip a disposable cotton swab into the bacterial suspension, and apply it on the culture medium without dripping liquid, at different angles, and apply it 3 times. Then use a sterilized disposable 200 μL pipette tip to punch holes appropriately on the surface of the petri dish, pick out the solid culture medium in the holes, and then dissolve and dilute the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap containing 0-4 parts by mass of ethyl acetate extract to a concentration of 1 mg / mL, and use a pipette gun to aspirate 100 μL each and inject it into the holes. The reference products are Manting soap and Liushen antibacterial soap sold on the market. Place each culture medium in an incubator at 37 °C and culture for 18 h - 24 h. The diameter of the antibacterial circle was measured by cross-crossing, and the average value of 3 parallel samples of the diameter of the antibacterial circle was taken as the measurement result.

[0080] The Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap of the present invention has a better inhibitory effect on Escherichia coli, Staphylococcus aureus and Candida albicans compared with the mainstream products on the market (Manting soap, Liushen antibacterial soap). The antibacterial effect is as Figure 2 、 Figure 3 shown.

[0081] 2. Foaming property test

[0082] Weigh 0.2 g of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap sample, dissolve it in 100 mL of pure water to prepare a soap sample solution. Take 10 mL and place it in a 50 mL centrifuge tube, and shake for 1 min to test the foam volume.

[0083] The foam of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap is relatively rich and the foam is dense. The results are as Figure 4 shown.

[0084] 3. Cleaning power test

[0085] Use a black marker pen to draw 3 solid circles of the same size on the forearm and make marks. No. 1 is the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap, No. 2 is Manting soap, and No. 3 is Liushen original liquid antibacterial soap.

[0086] The cleaning effect of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap is the best. The cleaning effect is as Figure 5 shown.

[0087] 4. Stability test

[0088] Weigh 5 g of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap, place it in an oven at 50 °C for 6 h, take it out and weigh it, which is the thermal stability of the cold-processed soap. Weigh 5 g of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap, place it in a 4 °C refrigerator for 6 h, take it out and weigh it, which is the cold stability of the cold-processed soap.

[0089] The results are shown in Table 1 below:

[0090] Table 1

[0091] Extract content (g) 0 1 2 3 4 Cold stability 100 100 100 100 100 Thermal stability 97.9 97.8 97.9 98.0 97.7

[0092] 5. Moisture and Volatiles Test

[0093] The oven method is adopted. The test is carried out in accordance with the standard of "QB / T2485-2008 Toilet Soap".

[0094] The results are shown in Table 2 below:

[0095] Table 2

[0096] Extract content (g) 0 1 2 3 4 Moisture and volatile matter (%) 5.3 6.9 6.3 7.2 6.3

[0097] All are less than 15%, meeting the national standard.

[0098] The present invention details the preparation method and performance test of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap. In the preparation of the Bletilla striata extract, through specific steps including drying and pulverizing the Bletilla striata tuber, ethanol soaking, vacuum concentration and multiple extractions, etc., the key raw materials for soap preparation are obtained. The preparation of the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap involves multiple embodiments. Each embodiment has differences in the formulation ratio of mixed oils, the preparation of lye, the saponification stirring time, the addition amount of extract and essential oil, etc., but generally follows a similar preparation process, including mixing oils, preparing lye, saponification, adding extract and essential oil, and molding and curing, etc., and finally obtaining cold-processed soap products with different formulations.

[0099] The performance tests cover antibacterial test, foaming property test, detergency test, stability test and moisture and volatiles test. The antibacterial test adopts the punching method. The results show that the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap has good inhibitory effects on Escherichia coli, Staphylococcus aureus and Candida albicans, superior to the mainstream products on the market. The foaming property test shows that the cold-processed soap has rich and dense foam. In the detergency test, its cleaning effect is the best. The stability test shows that the cold-processed soap has good cold and heat stability under different extract contents. The results of the moisture and volatiles test all meet the national standard, indicating that the Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap performs excellently in various performances and has good application potential.

[0100] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A traditional Chinese medicine cold-processed soap for inhibiting bacteria and skin care with Bletilla striata, characterized in that, The formula of the Bletilla striata antibacterial and skin care traditional Chinese medicine cold-processed soap is as follows: 1-4 parts by mass of Bletilla striata extract, 6-15 parts by mass of olive oil, 2-7 parts by mass of coconut oil, 2-7 parts by mass of palm oil, 2-4 parts by mass of sodium hydroxide, 4-6 parts by mass of ultrapure water, and 4 parts by mass of essential oil.

2. The cold-processed soap of traditional Chinese medicine for inhibiting bacteria and skin care with Bletilla striata according to claim 1, characterized in that, The essential oil is selected from one or more of the following: borneol essential oil, citrus essential oil, sweet orange essential oil.

3. The cold-processed soap of traditional Chinese medicine for inhibiting bacteria and skin care with Bletilla striata according to claim 1, characterized in that, The Bletilla striata extract includes one or more of the following: Bletilla striata petroleum ether extract, Bletilla striata dichloromethane extract, Bletilla striata ethyl acetate extract, Bletilla striata n-butanol extract.

4. The cold-processed soap of traditional Chinese medicine for inhibiting bacteria and skin care with Bletilla striata according to claim 3, wherein, The preparation method of the Bletilla striata extract is as follows: Crush and sieve the Bletilla striata tubers, soak them in 80% ethanol for 5-7 days, and collect the ethanol extract. Remove ethanol by rotary evaporation under reduced pressure to obtain the total extract. Dissolve the total extract in ultrapure water, extract with petroleum ether, dichloromethane, ethyl acetate or n-butanol respectively, collect the extracts, and dry them by rotary evaporation. That is, Bletilla striata petroleum ether extract, Bletilla striata dichloromethane extract, Bletilla striata ethyl acetate extract, and Bletilla striata n-butanol extract are obtained.

5. A Bletilla striata antibacterial and skin care traditional Chinese medicine cold-processed soap according to claim 1, characterized in that The weight mass fractions of the Bletilla striata extract are 1 part by mass, 2 parts by mass, 3 parts by mass, and 4 parts by mass respectively.

6. The cold-processed soap of traditional Chinese medicine for inhibiting bacteria and skin care with Bletilla striata according to claim 1, wherein, The Bletilla striata antibacterial and skin care traditional Chinese medicine cold-processed soap inhibits the growth of Escherichia coli, Candida albicans, and Staphylococcus aureus.

7. A Bletilla striata antibacterial skin care traditional Chinese medicine cold-processed soap according to claim 1, characterized in that The pH of the Bletilla striata antibacterial and skin care traditional Chinese medicine cold-processed soap is 6.0-8.

0.

8. The preparation method of a Bletilla striata antibacterial skin care traditional Chinese medicine cold-processed soap according to claim 1, characterized in that, The preparation method is as follows: Weigh olive oil, coconut oil, and palm oil, mix and stir evenly to obtain a mixed oil. Weigh sodium hydroxide and add it to ultrapure water to obtain an alkali solution. Preheat the mixed oil and the alkali solution to 40-50 °C, pour the alkali solution into the mixed oil, keep the temperature at 40-50 °C, and stir with a stirrer for 2-4 h to saponify it. Weigh the Bletilla striata extract and put it into the above saponification solution and continue to stir evenly. Weigh the essential oil, put it into the soap solution, and continue to stir evenly. When the soap solution can be stirred to draw an 8 shape and does not disappear within 5-10 seconds, it can be put into the mold. After putting it into the mold, demold and cure it to obtain the Bletilla striata antibacterial and skin care traditional Chinese medicine cold-processed soap.

9. The preparation method of a Bletilla striata antibacterial skin care traditional Chinese medicine cold-processed soap according to claim 6, characterized in that, After putting it into the mold, keep it warm for 24 hours, demold it after 2-3 days, and cure it in the dark at a constant temperature and humidity for at least 1 month.

10. The preparation method of a Bletilla striata antibacterial and skin-care traditional Chinese medicine cold-processed soap according to claim 7, characterized in that The constant temperature condition is 28 °C - 30 °C, and the constant humidity condition is 40% - 50%.

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