Full-automatic bacterial growth detection system and detection method thereof
Through the fully automatic bacterial growth detection system, the motor-driven horizontal mover and lifter are used to realize automated sample collection and detection, solving the time-consuming and error problems caused by manual intervention in traditional detection, and achieving efficient and accurate bacterial growth detection.
Patent Information
- Application Number
- CN202510760822.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-09
- Publication Date
- 2025-07-08
AI Technical Summary
Traditional bacterial growth detection requires frequent manual intervention, which makes it time-consuming and labor-intensive and easy to introduce operational errors, affecting the accuracy of the detection results.
A fully automatic bacterial growth detection system is designed, including a detection box, a sample incubator, absorption assembly, a test table and a cleaning assembly. It realizes automated sample collection, detection and cleaning through a motor-driven horizontal mover and lifter, and combines a dilution head and agitator to improve detection efficiency and accuracy.
It realizes full automation of bacterial growth detection, improves work efficiency and detection accuracy, and reduces the error caused by manual intervention.
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Figure CN120272310A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of microbial devices, and in particular to a fully automatic bacterial growth detection system and a detection method thereof. Background Art
[0002] Bacterial growth detection is an experimental technique for evaluating the reproduction rate and quantity change of bacteria under specific conditions. Such detections can help understand the influence of different environmental factors such as nutrients, temperature, pH value, etc. on bacterial growth, and are widely used in fields such as food hygiene, medical diagnosis, pharmaceutical industry, and environmental monitoring. Through detection, the growth curve of bacteria can be quantitatively analyzed, including four stages: lag phase, logarithmic phase, stationary phase, and decline phase, thus providing an important basis for scientific research and practical applications.
[0003] In the traditional OD value measurement process, samples need to be manually collected, and the samples are transferred from the culture environment to the measuring instrument for measurement. This means that if continuous monitoring of the bacterial growth situation is to be achieved, staff need to repeat this operation process regularly. Frequent manual intervention not only takes time and effort, but also easily affects the accuracy of experimental results due to operation errors or contamination. Summary of the Invention
[0004] The purpose of the present invention is to provide a fully automatic bacterial growth detection system and a detection method thereof, aiming to automatically and continuously collect samples during the bacterial production process for detection, thereby improving the detection efficiency.
[0005] To achieve the above purpose, in the first aspect, the present invention provides a fully automatic bacterial growth detection system, including a detection box, a sample culture box, a cover plate, a liquid suction component, a detection table, a detection multi-well plate, a detection unit, and a cleaning component. The sample culture box is arranged inside the detection box, the cover plate is slidably arranged on the top of the sample culture box, the liquid suction component includes a horizontal mover, a first lifter, a support block, a pressure pump, and a suction pipe. The horizontal mover is slidably arranged inside the detection box, the first lifter is arranged on the horizontal mover, the support block is connected to the output end of the first lifter, the suction pipe is arranged on the support block, and the pressure pump is communicated with the suction pipe. The detection table is arranged on one side of the sample culture box, the detection multi-well plate is arranged on the detection table, and the detection unit is arranged below the detection table. The cleaning component includes a multi-way valve, a cleaning box, a cleaning pump, and a collection box. The multi-way valve is communicated with the suction pipe, the cleaning pump is communicated with the multi-way valve, the cleaning box is communicated with the cleaning pump, and the collection box is arranged on one side of the detection table.
[0006] Among them, the horizontal mover includes a first motor, a screw rod, and a moving block. The first motor is fixed inside the detection box. The screw rod is fixedly connected to the output end of the first motor. The moving block is threadedly connected to the screw rod. The first lifter is arranged on the moving block.
[0007] Among them, the liquid suction assembly further includes a stirrer. The stirrer is arranged on one side of the support block and is used for stirring the sample in the sample incubator when sampling with the pipette.
[0008] Among them, the stirrer includes a second lifter, a stirring motor, a stirring rod, and stirring blades. The second lifter is arranged on the support block. The stirring motor is fixed to the output end of the second lifter. The stirring rod is connected to the output end of the stirring motor. The stirring blades are connected to the stirring rod.
[0009] Among them, the liquid suction assembly further includes a dilution head. The dilution head is rotatably arranged on the support block and is located below the pipette.
[0010] Among them, the dilution head includes a driver, a rotating rod, a sliding block, a dilution head body, and a valve. The rotating rod is rotatably arranged on one side of the support block. The driver is used to drive the rotating rod to rotate. The sliding block is slidably arranged on the rotating rod. The dilution head body is fixed on the sliding block. The valve is arranged at the bottom of the dilution head body.
[0011] Among them, the dilution head further includes a docking head. The docking head is fixed on the top of the dilution head body. The pipette is provided with a sealing sleeve corresponding to the docking head. The sealing sleeve has a first cavity. A chamfer is provided at the position of the first cavity close to the docking head. A conical sealing ring is arranged in the first cavity. A spiral inner ring is arranged on the inner wall of the dilution head body. A rotating rod, a threaded rod, and a stirring rod are arranged in the first cavity. The rotating rod is rotatably arranged in the first cavity. The threaded rod is fixed on the rotating rod and matches the spiral inner ring. The stirring rod is arranged at the bottom of the threaded rod. A plurality of flow holes are arranged on the threaded rod.
[0012] Among them, the dilution head further includes an elastic member. The elastic member is arranged between the sliding block and the rotating rod.
[0013] Among them, the cleaning assembly further includes an air pipe, a heater, and an air pump. The air pipe is communicated with the multi-way valve. The air pump is communicated with the air pipe. The heater is arranged on one side of the air pipe.
[0014] In a second aspect, the present invention further provides a full-automatic bacterial growth detection method, which uses the full-automatic bacterial growth detection system described above.
[0015] A fully automatic bacterial growth detection system and its detection method of the present invention. Inside the detection box, there is a sample incubator for storing bacterial samples to be detected. For convenient operation, the cover plate is slidably arranged on the top of the sample incubator, so that it can be easily opened or closed to facilitate the addition or removal of samples.
[0016] The horizontal mover of the liquid suction assembly allows the liquid suction assembly to slide freely in the detection box, so that it can reach any position that needs to be processed. The first lifter is installed on the horizontal mover and can adjust the position of the support block by moving up and down, and the support block is directly connected to the suction pipe. The pressure pump is connected to the suction pipe through a pipeline and can generate negative pressure when needed to suck the liquid sample.
[0017] The detection table is located on one side of the sample incubator, and a detection multi-well plate is placed thereon for carrying different bacterial samples. The detection unit is arranged below the detection table and is responsible for real-time monitoring and data analysis of the samples on the multi-well plate to evaluate the growth state of bacteria. A slidable light source is arranged above the detection table, and the samples on the detection multi-well plate can be irradiated through the light source, so that the detection unit at the bottom can evaluate the growth state of bacteria according to the received light conditions.
[0018] The cleaning assembly consists of a multi-way valve, a cleaning box, a cleaning pump and a collection box. The multi-way valve is connected to the suction pipe of the liquid suction assembly, so that after a liquid suction operation is completed, the cleaning liquid can be pumped from the cleaning box by switching the valve and transported to the suction pipe through the cleaning pump to achieve automatic cleaning. The waste liquid after use will be guided to the collection box to avoid cross-contamination.
[0019] In summary, this fully automatic bacterial growth detection system realizes the full automation of bacterial growth detection through the close cooperation between components, greatly improves the work efficiency and accuracy, and at the same time reduces the errors that may be brought by manual intervention. Brief Description of the Drawings
[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.
[0021] Figure 1 It is a structural diagram of a fully automatic bacterial growth detection system of the present invention.
[0022] Figure 2 It is an internal structural diagram of a fully automatic bacterial growth detection system of the present invention.
[0023] Figure 3 It is the second internal structure diagram of a fully automatic bacterial growth detection system of the present invention.
[0024] Figure 4 It is the sectional structure diagram of a fully automatic bacterial growth detection system of the present invention.
[0025] Figure 5 It is the sectional structure diagram of a fully automatic bacterial growth detection system of the present invention along the pipette.
[0026] Figure 6 is Figure 5 The partial enlarged view of detail A.
[0027] Detection box 101, sample incubation box 102, cover plate 103, liquid suction assembly 104, detection table 105, detection multi-well plate 106, detection unit 107, cleaning assembly 108, horizontal mover 109, first lifter 110, support block 111, pressure pump 112, pipette 113, multi-way valve 114, cleaning box 115, cleaning pump 116, collection box 117, first motor 118, screw 119, moving block 120, second lifter 121, stirring motor 122, stirring rod 123, stirring blade 124, driver 125, rotating rod 126, sliding block 127, dilution head body 128, valve 129, docking head 130, sealing sleeve 131, elastic member 132, air pipe 133, heater 134, air pump 135, chamfer 136, conical sealing ring 137, spiral inner ring 138, rotating rod 139, threaded rod 140, stirring rod 141. Specific embodiments
[0028] The embodiments of the present invention will be described in detail below. Examples of the embodiments are shown in the drawings, where the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.
[0029] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. In addition, in the description of the present invention, the meaning of "a plurality of" is two or more unless otherwise specifically defined.
[0030] The first embodiment Please refer to Figures 1 to 6 , the present invention provides a fully automatic bacterial growth detection system, including a detection box 101, a sample incubator 102, a cover plate 103, a liquid suction assembly 104, a detection table 105, a detection multi-well plate 106, a detection unit 107 and a cleaning assembly 108. The sample incubator 102 is arranged inside the detection box 101, the cover plate 103 is slidably arranged on the top of the sample incubator 102, the liquid suction assembly 104 includes a horizontal mover 109, a first lifter 110, a support block 111, a pressure pump 112 and a suction pipe 113. The horizontal mover 109 is slidably arranged inside the detection box 101, the first lifter 110 is arranged on the horizontal mover 109, the support block 111 is connected to the output end of the first lifter 110, the suction pipe 113 is arranged on the support block 111, and the pressure pump 112 is communicated with the suction pipe 113. The detection table 105 is arranged on one side of the sample incubator 102, the detection multi-well plate 106 is arranged on the detection table 105, and the detection unit 107 is arranged below the detection table 105. The cleaning assembly 108 includes a multi-way valve 114, a cleaning box 115, a cleaning pump 116 and a collection box 117. The multi-way valve 114 is communicated with the suction pipe 113, the cleaning pump 116 is communicated with the multi-way valve 114, the cleaning box 115 is communicated with the cleaning pump 116, and the collection box 117 is arranged on one side of the detection table 105.
[0031] In this embodiment, a sample incubator 102 is arranged inside the detection box 101 for storing bacterial samples to be detected. For convenient operation, the cover plate 103 is slidably arranged on the top of the sample incubator 102, so that it can be easily opened or closed to facilitate adding or removing samples.
[0032] The horizontal mover 109 of the liquid suction assembly 104 allows the liquid suction assembly 104 to freely slide inside the detection box 101, so that it can reach any position that needs to be processed. The first lifter 110 is installed on the horizontal mover 109 and can adjust the position of the support block 111 by moving up and down, and the support block 111 is directly connected to the suction pipe 113. The pressure pump 112 is connected to the suction pipe 113 through a pipeline and can generate negative pressure when needed to suck liquid samples.
[0033] The detection platform 105 is located on one side of the sample incubator 102, and a detection multi-well plate 106 is placed thereon for carrying different bacterial samples. The detection unit 107 is arranged below the detection platform 105 and is responsible for real-time monitoring and data analysis of the samples on the multi-well plate to evaluate the growth state of bacteria. A slidable light source is arranged above the detection platform 105, and the samples on the detection multi-well plate 106 can be irradiated through the light source, so that the detection unit 107 at the bottom can evaluate the growth state of bacteria according to the received light conditions.
[0034] The cleaning component 108 consists of a multi-way valve 114, a cleaning tank 115, a cleaning pump 116 and a collection tank 117. The multi-way valve 114 is communicated with the suction pipe 113 of the liquid suction component 104, so that after a liquid suction operation is completed, the cleaning liquid can be extracted from the cleaning tank 115 by switching the valve 129 and conveyed to the suction pipe 113 through the cleaning pump 116 to realize automatic cleaning. The waste liquid after use will be guided to the collection tank 117 to avoid cross-contamination.
[0035] In summary, this fully automatic bacterial growth detection system realizes the full automation of bacterial growth detection through the close cooperation between components, greatly improves the work efficiency and accuracy, and at the same time reduces the errors that may be brought by manual intervention.
[0036] The horizontal mover 109 includes a first motor 118, a screw rod 119 and a moving block 120. The first motor 118 is fixed inside the detection box 101. The screw rod 119 is fixedly connected to the output end of the first motor 118. The moving block 120 is threadedly connected to the screw rod 119, and the first lifter 110 is arranged on the moving block 120.
[0037] The first motor 118 is the power source of the horizontal mover 109 and is firmly fixed at a suitable position inside the detection box 101. By selecting a suitable motor model, it can be ensured that sufficient torque and rotational speed are provided to drive the mechanical structure described next, so as to meet the requirements in different working environments. One end of the screw rod 119 is directly fixedly connected to the output shaft of the first motor 118, so that when the motor starts, the screw rod 119 can be driven to rotate synchronously. The inner part of the moving block 120 is designed with an internal thread that matches the external thread of the screw rod 119, so that when the screw rod 119 rotates, the moving block 120 can move linearly along the axial direction of the screw rod 119. This design not only realizes precise position control but also can bear a certain load, ensuring the stability of the equipment during the process of sucking or releasing liquid. The first lifter 110 is arranged on the moving block 120. This means that as the moving block 120 moves along the screw rod 119, the first lifter 110 can also reach any specified position inside the detection box 101.
[0038] The liquid suction assembly 104 further includes a stirrer, which is arranged on one side of the support block 111 and is used for stirring the sample in the sample incubator 102 when sampling with the suction pipe 113.
[0039] The stirrer includes a second lifter 121, a stirring motor 122, a stirring rod 123 and stirring blades 124. The second lifter 121 is arranged on the support block 111. The stirring motor 122 is fixed to the output end of the second lifter 121. The stirring rod 123 is connected to the output end of the stirring motor 122. The stirring blades 124 are connected to the stirring rod 123.
[0040] The second lifter 121 is arranged on the support block 111 and is responsible for controlling the vertical position adjustment of the stirrer. With this design, the height of the stirrer can be flexibly adjusted according to different experimental requirements, enabling it to adapt to sample containers of different depths. The addition of the second lifter 121 not only improves the adaptability of the system but also ensures the accuracy of the stirring operation.
[0041] The stirring motor 122 is the power source of the stirrer. The stirring motor 122 is firmly fixed to the output end of the second lifter 121. When stirring is required, the second lifter 121 first adjusts the stirring motor 122 to the appropriate height, and then the stirring motor 122 starts to provide the necessary rotational power. The stirring rod 123 is directly connected to the output end of the stirring motor 122 and is a key component for transmitting the stirring force. To meet the requirements under different experimental conditions, the design of the stirring rod 123 needs to consider both strength and flexibility, being able to withstand sufficient torque without affecting the stirring effect due to excessive rigidity. The stirring blades 124 are connected to the stirring rod 123 and are the part directly in contact with the sample. The design of the stirring blades 124 directly affects the stirring effect. Generally, the stirring blades 124 will adopt specific shapes and sizes to ensure the most effective mixing without damaging the sample.
[0042] The liquid suction assembly 104 further includes a dilution head, which is rotatably arranged on the support block 111 and is located below the suction pipe 113.
[0043] To make the detection more stable, a liquid for dilution can be added to the dilution head, and the suction pipe 113 is connected to the dilution head for mixing, and then the detection is carried out.
[0044] The dilution head includes a driver 125, a rotating rod 126, a sliding block 127, a dilution head body 128, and a valve 129. The rotating rod 126 is rotatably arranged on one side of the support block 111. The driver 125 is used to drive the rotation of the rotating rod 126. The sliding block 127 is slidably arranged on the rotating rod 126. The dilution head body 128 is fixed on the sliding block 127. The valve 129 is arranged at the bottom of the dilution head body 128.
[0045] The driver 125 is the power source of the dilution head and is responsible for driving the rotational movement of the rotating rod 126. The rotating rod 126 is rotatably arranged on one side of the support block 111 and is a key component connecting the driver 125 and the sliding block 127. It not only needs to have sufficient strength to support the sliding block 127 and the dilution head body 128 thereon but also needs to ensure the smoothness of the rotational movement to avoid position deviation caused by friction or mechanical errors.
[0046] The sliding block 127 is slidably arranged on the rotating rod 126, allowing the dilution head body 128 to adjust its position in the vertical direction. The dilution head body 128 is fixed on the sliding block 127 and is the core component directly involved in the dilution work. The valve 129 is arranged at the bottom of the dilution head body 128 and can be opened after a certain pressure is applied. When in use, the driver 125 is started to drive the rotating rod 126 to rotate by a preset angle, so that the dilution head body 128 is aligned with the pipette 113. Then, the pipette 113 descends under the action of the first lifter 110 to dock with the dilution head body 128. Then, the sample is injected, and air is continuously injected until the pressure reaches the preset value and the valve 129 opens, so that the diluted sample can be discharged.
[0047] The dilution head further includes a docking head 130. The docking head 130 is fixed on the top of the dilution head body 128. The pipette 113 is provided with a sealing sleeve 131 corresponding to the docking head 130. The sealing sleeve 131 has a first cavity. A chamfer 136 is provided at the position of the first cavity close to the docking head 130. A conical sealing ring 137 is arranged in the first cavity. A spiral inner ring 138 is arranged on the inner wall of the dilution head body 128. A rotating rod 126, a threaded rod 140, and a stirring rod 141 are arranged in the first cavity. The rotating rod 126 is rotatably arranged in the first cavity. The threaded rod 140 is fixed on the rotating rod 126 and matches with the spiral inner ring 138. The stirring rod 141 is arranged at the bottom of the threaded rod 140. A plurality of flow holes are arranged on the threaded rod 140.
[0048] The docking head 130 is fixed to the top of the dilution head body 128. The design of the docking head 130 is aimed at precisely docking with the sealing sleeve 131 at the end of the pipette 113, so as to achieve the best sealing effect during the liquid transfer process, avoiding leakage or external contamination. The sealing sleeve 131 is correspondingly arranged at the end of the pipette 113 and is usually made of a chemical-resistant and highly elastic material such as silica gel or fluororubber.
[0049] In addition, in order to enable the sealing sleeve 131 to accurately dock with the docking head 130, a chamfer 136 is provided in the first cavity of the sealing sleeve 131, so that the sealing sleeve 131 can have a larger tolerance space to align with the docking head 130. After docking, in order to enable the sample and the diluent to be more fully mixed to improve the uniformity of the sample distribution, a rotating rod 126 is provided in the sealing sleeve 131. When the sealing sleeve 131 moves downward during docking, the threaded rod 140 contacts the spiral inner ring 138 on the inner wall of the dilution head body 128, thereby driving the threaded rod 140 to rotate, so as to drive the stirring rod 141 to rotate, thereby accelerating the liquid mixing in the dilution head body 128 and improving the uniformity.
[0050] The dilution head further includes an elastic member 132, and the elastic member 132 is arranged between the sliding block 127 and the rotating rod 126.
[0051] The elastic member 132 is cleverly arranged between the sliding block 127 and the rotating rod 126, providing additional flexibility and stability for the operation of the dilution head. The functions of the elastic member 132 are mainly reflected in two aspects: on the one hand, during the up and down movement of the sliding block 127, the elastic member 132 can provide a buffering effect, reducing mechanical wear and protecting the equipment; on the other hand, when the sliding block 127 reaches the designated position, the elastic member 132 can provide a certain pre-tightening force, enabling the dilution head body 128 to stay more firmly in the target position, ensuring that the contact between the dilution head and the sample container is both tight and does not damage the container.
[0052] The cleaning assembly 108 further includes an air pipe 133, a heater 134 and an air pump 135. The air pipe 133 is communicated with the multi-way valve 114, the air pump 135 is communicated with the air pipe 133, and the heater 134 is arranged on one side of the air pipe 133.
[0053] The trachea 133 is connected to the multi-way valve 114. Through such a design, it is possible to flexibly switch the input paths of liquids or gases in different cleaning steps, thereby achieving a comprehensive cleaning of the internal pipes and components of the system. The air pump 135 is a key device that provides power to the trachea 133. The air pump 135 is connected to the trachea 133 and can inject compressed air into the system when needed. This step is particularly important for removing residual substances attached to the surface of the straw 113 or other components.
[0054] The heater 134 is arranged on one side of the trachea 133 and is used to heat the gas passing through the trachea 133. The presence of the heater 134 enables the system to adjust the gas temperature according to specific experimental requirements, which helps to dry the components after cleaning and avoid the influence of water stains on subsequent experimental results.
[0055] Second Embodiment The present invention also provides a fully automatic method for detecting bacterial growth, using the described fully automatic bacterial growth detection system.
[0056] In the preparation stage, the bacterial sample to be detected needs to be placed in the sample incubator 102 and closed by the sliding cover plate 103 to maintain a stable environment. At this time, appropriate temperature, humidity and other necessary growth conditions can be set to provide the best environment for the growth of bacteria.
[0057] The first motor 118 drives the screw 119 on the horizontal mover 109 to rotate, so that the moving block 120 moves along the screw 119 to the designated position. Then, the first lifter 110 adjusts the height of the support block 111 to accurately align the straw 113 with the target sample. During this process, the stirrer can be lowered to an appropriate position by the second lifter 121, and the stirrer motor 122 is used to drive the stirrer rod 123 and the blades to uniformly stir the sample to ensure the representativeness of sampling. After that, the pressure pump 112 is started, and a certain amount of sample liquid is sucked through the straw 113.
[0058] For samples that need to be diluted, the dilution head controls the rotation of the rotating rod 126 through the driver 125 to adjust the position of the sliding block 127, so that the dilution head body 128 reaches the predetermined position. After the docking head 130 is accurately docked with the sealing sleeve 131 at the end of the straw 113, the valve 129 is opened to allow the sample to enter the inside of the dilution head for dilution at a preset ratio. The presence of the elastic member 132 ensures the stability and accuracy throughout the process.
[0059] The processed samples are transferred onto the detection porous plate 106 and placed on the detection table 105. The detection unit 107 is located below the detection table 105 and begins to conduct real-time monitoring of the samples in each well. This includes but is not limited to optical density (OD value) measurement, fluorescence analysis, etc., to evaluate the growth rate and quantity change of bacteria. All data will be automatically recorded and transmitted to the computer system for subsequent analysis.
[0060] The cleaning stage is a crucial step to ensure the continuous and efficient operation of the system. The cleaning component 108 switches different working modes through the multi-way valve 114. First, the cleaning pump 116 extracts the cleaning liquid in the cleaning tank 115, and is pushed by the compressed air provided by the air pipe 133 and the air pump 135 to thoroughly rinse the liquid suction component 104 and other related components. The heater 134 can adjust the temperature of the cleaning liquid as needed to further enhance the cleaning effect. After cleaning, the waste liquid is collected in the collection tank 117 to avoid cross-contamination.
[0061] In the data analysis stage, the collected data is processed and analyzed through dedicated software to generate detailed reports. These reports can help researchers understand the growth characteristics of bacteria under different conditions and support various application scenarios such as scientific research or quality control.
[0062] The above-disclosed is only a preferred embodiment of the present invention. Of course, it cannot be used to limit the scope of the rights of the present invention. Those of ordinary skill in the art can understand the whole or part of the process of implementing the above embodiment, and the equivalent changes made according to the claims of the present invention still fall within the scope covered by the invention.
Claims
1. An automatic bacterial growth detection system, comprising a detection box, a sample culture box and a cover plate. The sample culture box is arranged inside the detection box, and the cover plate is slidably arranged on the top of the sample culture box. It is characterized in that, it further comprises a liquid suction assembly, a detection table, a detection multi-well plate, a detection unit and a cleaning assembly; The liquid suction assembly includes a horizontal mover, a first lifter, a support block, a pressure pump and a suction pipe. The horizontal mover is slidably arranged inside the detection box. The first lifter is arranged on the horizontal mover. The support block is connected to the output end of the first lifter. The suction pipe is arranged on the support block. The pressure pump is communicated with the suction pipe; The detection table is arranged on one side of the sample culture box. The detection multi-well plate is arranged on the detection table. The detection unit is arranged below the detection table; The cleaning assembly includes a multi-way valve, a cleaning box, a cleaning pump and a collection box. The multi-way valve is communicated with the suction pipe. The cleaning pump is communicated with the multi-way valve. The cleaning box is communicated with the cleaning pump. The collection box is arranged on one side of the detection table.
2. The automatic bacterial growth detection system according to claim 1, characterized in that, The horizontal mover includes a first motor, a screw rod and a moving block. The first motor is fixed inside the detection box. The screw rod is fixedly connected to the output end of the first motor. The moving block is threadedly connected to the screw rod. The first lifter is arranged on the moving block.
3. The automatic bacterial growth detection system according to claim 2, characterized in that, The liquid suction assembly further includes a stirrer. The stirrer is arranged on one side of the support block and is used for stirring the sample in the sample culture box when sampling with the suction pipe.
4. The automatic bacterial growth detection system according to claim 3, characterized in that, The stirrer includes a second lifter, a stirring motor, a stirring rod and stirring blades. The second lifter is arranged on the support block. The stirring motor is fixed to the output end of the second lifter. The stirring rod is connected to the output end of the stirring motor. The stirring blades are connected to the stirring rod.
5. The automatic bacterial growth detection system according to claim 4, characterized in that, The liquid suction assembly further includes a dilution head. The dilution head is rotatably arranged on the support block and is located below the suction pipe.
6. The automatic bacterial growth detection system according to claim 5, characterized in that, The dilution head includes a driver, a rotating rod, a sliding block, a dilution head body and a valve. The rotating rod is rotatably arranged on one side of the support block. The driver is used for driving the rotating rod to rotate. The sliding block is slidably arranged on the rotating rod. The dilution head body is fixed on the sliding block. The valve is arranged at the bottom of the dilution head body.
7. The automatic bacterial growth detection system according to claim 6, characterized in that, The dilution head further includes a docking head which is fixed to the top of the dilution head body. A sealing sleeve is provided corresponding to the docking head on the pipette. The sealing sleeve has a first cavity. A chamfer is provided at a position close to the docking head in the first cavity. A conical sealing ring is arranged in the first cavity. A spiral inner ring is arranged on the inner wall of the dilution head body. A rotating rod, a threaded rod and a stirring rod are arranged in the first cavity. The rotating rod is rotatably arranged in the first cavity. The threaded rod is fixed on the rotating rod and matches with the spiral inner ring. The stirring rod is arranged at the bottom of the threaded rod. A plurality of flow holes are provided on the threaded rod.
8. The full-automatic bacterial growth detection system according to claim 7, wherein the dilution head further includes an elastic member which is arranged between the sliding block and the rotating rod.
9. The full-automatic bacterial growth detection system according to claim 8, wherein the cleaning assembly further includes an air pipe, a heater and an air pump. The air pipe is communicated with the multi-way valve. The air pump is communicated with the air pipe. The heater is arranged on one side of the air pipe.
10. A fully automatic method for detecting bacterial growth, characterized in that, Use the full-automatic bacterial growth detection system according to any one of claims 1 to 9.
Citation Information
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