A strain of Bacillus funiculosus and its application in promoting growth and reducing fertilizer use

Abstract

The present invention relates to the field of microbial technology, and in particular to a strain of Bacillus funiculosus and its application in promoting growth and reducing the use of chemical fertilizers. The strain B267 of the present invention was isolated by a research team from the rhizosphere soil of pears with long-term potassium deficiency. It has good IAA production and potassium solubilization capabilities. Plate tests found that the volatile organic compounds and secretions produced by it can effectively promote the development of the root system and aboveground parts of Arabidopsis thaliana. Potted plant experiments found that B267 can significantly improve the root development morphology of tomato and pear seedlings and enhance the root system's K tolerance. + It can increase the absorption rate of B267, improve the biomass of seedlings, tomato yield and quality, and has a good growth-promoting effect on plants. After inoculation with B267 strain, it can replace chemical potassium fertilizer to a certain extent, reduce fertilizer input, and provide more theoretical support and technical means for the development of green agriculture.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, in particular to a strain of Bacillus funiculosus and its application in promoting growth and reducing the amount of chemical fertilizers. Background Art

[0002] Potassium is one of the key mineral elements that are indispensable for plant growth and development. It participates in a variety of physiological and biochemical processes in plants, including photosynthesis, carbohydrate metabolism, protein synthesis, and cell osmotic regulation. Potassium in soil exists mainly in three forms: mineral potassium (potassium bound in mineral crystals), exchangeable potassium (potassium adsorbed on soil colloids), and potassium in the form of potassium. + ) and water-soluble potassium (K dissolved in soil solution + ). However, among these forms of potassium, only water-soluble potassium can be directly absorbed and utilized by plants. Due to the influence of factors such as soil pH, texture and organic matter content, a large amount of potassium often exists in a fixed form, resulting in a low utilization rate. This not only limits the absorption of potassium by crops, but may also lead to an imbalance of soil nutrients, thereby affecting crop yield and quality. In the traditional agricultural production model, the long-term excessive use of chemical fertilizers has caused many negative impacts on the ecological environment and seriously threatened the sustainable development of agriculture. At a time when green agriculture has become an inevitable trend in the development of the industry, the use of beneficial microorganisms to maintain healthy plant growth, improve nutrient absorption efficiency, and increase crop yields has become an effective means to replace traditional fertilization methods.

[0003] Traditional fertilization methods often use a single chemical fertilizer for supplementation. This method has problems such as large dosage, high cost, single nutrient content, and the tendency to cause soil compaction and secondary salinization. Long-term use of chemical fertilizers may also destroy the balance of soil microecology, reduce the sustainability of soil fertility, and pose a risk of environmental pollution. In contrast, Bacillaceae ( Bacillaceae Certain strains of Bacillus spp. (Bacillus sp.) have shown great potential in agriculture. These microorganisms secrete extracellular enzymes to decompose organic matter, releasing soluble potassium ions. They also regulate soil pH, improving potassium availability. Furthermore, Bacillus sp. promotes plant root development, enhances crop resistance to stress, and inhibits pathogen growth through antagonistic effects. Combining these functions with traditional fertilization can not only improve fertilizer utilization efficiency but also achieve the goal of green and sustainable agriculture.

[0004] The genus Bacillus is a new genus separated from the genus Bacillus in recent years and belongs to the Bacillaceae family ( Bacillaceae ), a research team from McMaster University in Canada found through comprehensive phylogenetic analysis that members of the traditional Bacillus genus have significant differences at the genomic level and cannot be attributed to a single evolutionary branch. Therefore, it was split into 17 new genera, including EctobacillusAt present, the research on this genus is mainly concentrated in the fields of wastewater treatment and bioremediation. However, the research on the role of Bacillus fungi in potassium dissolution and growth promotion in fruit trees, as well as its role in plant growth promotion, is still relatively scarce. In-depth research is urgently needed to reveal its potential value and provide more theoretical support and technical means for the development of green agriculture. Summary of the Invention

[0005] In view of the above, it is necessary to conduct in-depth research on Bacillus funiculosus and develop new strains, mainly studying the role of new strains in potassium dissolution and growth promotion in fruit trees. In-depth research should be conducted to reveal its potential value and provide more theoretical support and technical means for the development of green agriculture.

[0006] In order to achieve the above purpose, the present invention screened out a new strain: Bacillus funiculosus Ectobacillus sp. strain B267, which is classified as: Ectobacillus sp. B267, Chinese classification name: Bacillus funiculosus B267, preservation number: CCTCC NO: M2025411; the strain is deposited in the China Center for Type Culture Collection, address: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the preservation date is March 10, 2025.

[0007] The present invention also includes the funicular Bacillus Ectobacillus sp. strain B267.

[0008] The present invention also includes the funicular Bacillus Ectobacillus Application of sp. strain B267 or the bacterial agent in the preparation of biological organic fertilizer.

[0009] The present invention also includes the funicular Bacillus Ectobacillus Application of sp. strain B267 or the bacterial agent in promoting plant growth.

[0010] Furthermore, the plant is Arabidopsis thaliana, tomato and / or Pyrus betula.

[0011] The present invention also includes the funicular Bacillus Ectobacillus Application of sp. strain B267 or the bacterial agent in producing IAA and / or potassium solution.

[0012] The present invention also includes the funicular Bacillus Ectobacillus Application of sp. strain B267 or the bacterial agent in reducing the amount of chemical fertilizers.

[0013] The present invention also includes the funicular Bacillus Ectobacillus Application of sp. strain B267 or the bacterial agent in improving the quality of tomato fruits.

[0014] Furthermore, the tomato fruit quality includes: tomato fruit size, fruit yield, fruit vitamin C content and / or fruit sucrose content.

[0015] The present invention also includes the use of the funicular Bacillus Ectobacillus sp. strain B267 or the method of promoting plant growth by the bacterial agent, the method comprising: the funicular Bacillus sp. Ectobacillus The bacterial suspension and / or fermentation liquid of sp. strain B267 is poured into the rhizosphere of the plant seedlings.

[0016] Furthermore, the preparation method of the fermentation broth is as follows: Ectobacillus sp. strain B267 was inoculated into TSB medium, and the supernatant was extracted by centrifugation after 24 hours. The supernatant was rotary evaporated and then diluted with sterile water.

[0017] The present invention has the following beneficial effects: the strain B267 of the present invention was isolated by the research team from the rhizosphere soil of pears with long-term potassium deficiency, and has good IAA production and potassium solubilization capabilities. Plate tests found that the volatile organic compounds and secretions produced by it can effectively promote the development of the root system and aboveground parts of Arabidopsis thaliana. Potted plant experiments found that B267 can significantly improve the root development morphology of tomato and pear seedlings, and enhance the root system's K + It can increase the absorption rate of B267, improve the biomass of seedlings, fruit yield and quality, and has a good growth-promoting effect on plants. After inoculation with B267 strain, it can replace chemical potassium fertilizer to a certain extent, reduce fertilizer input, and provide more theoretical support and technical means for the development of green agriculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Figure 2 is the colony morphology of strain B267.

[0019] Figure 2 This is the phylogenetic tree of strain B267.

[0020] Figure 3 This is the result graph of the potassium-solubilizing ability of strain B267.

[0021] Figure 4 This is the result of the effect of strain B267 on the root growth of Arabidopsis tissue culture seedlings.

[0022] Figure 5 This is a graph showing the effect of strain B267 on the growth of Arabidopsis seedlings; in the graph, A is the root dry weight of the seedlings, B is the aboveground dry weight, and C is the total dry weight.

[0023] Figure 6 This is the morphological result of the effect of strain B267 on the root growth of Arabidopsis seedlings.

[0024] Figure 7This is a graph showing the effect of strain B267 on the root system of Arabidopsis seedlings; in the figure, A is the root length result, B is the root surface area result, and C is the root tip number result.

[0025] Figure 8 The phenotypic results of different concentrations of secretions from strain B267 on the growth of Arabidopsis seedlings.

[0026] Figure 9 The results of different concentrations of secretions of strain B267 on the growth of Arabidopsis seedlings; in the figure, A is the root dry weight result, B is the aboveground dry weight result, and C is the total dry weight result.

[0027] Figure 10 The results of different concentrations of secretions of strain B267 on the root growth of Arabidopsis seedlings; in the figure, A is the root length result, B is the root surface area result, and C is the root tip number result.

[0028] Figure 11 This is the phenotypic result of strain B267 on the growth of tomato potted seedlings.

[0029] Figure 12 This is a graph showing the effect of strain B267 on tomato seedling growth; in the graph, A is the root dry weight of the seedling, B is the aboveground dry weight, and C is the total dry weight.

[0030] Figure 13 This is a diagram showing the phenotypic results of the effect of strain B267 on tomato roots.

[0031] Figure 14 These are the results of the effect of strain B267 on tomato root growth; in the figure, A is the root length result, B is the root surface area result, and C is the root tip number result.

[0032] Figure 15 This is the phenotypic result diagram of the effect of strain B267 on the growth of Pyrus betulae potted seedlings.

[0033] Figure 16 This is a graph showing the effect of strain B267 on the growth of Pyrus betulae seedlings; in the graph, A is the root dry weight of the seedlings, B is the aboveground dry weight, and C is the total dry weight.

[0034] Figure 17 This is a diagram showing the phenotypic results of the effect of strain B267 on the root system of Pyrus betula.

[0035] Figure 18 The results of strain B267 on the root growth of Pyrus betulaefolia are shown in the figure. In the figure, A is the root length, B is the root surface area, and C is the root tip number.

[0036] Figure 19 The strain B267 was used to treat the root system of Pyrus betula + Result graph of absorption rate.

[0037] Figure 20 This is the phenotypic result diagram of strain B267 on tomato potted yield.

[0038] Figure 21 This is a graph showing the effect of strain B267 on tomato quality; in the figure, A is the yield per plant, B is the soluble solids content, C is the vitamin C content, D is the fructose content, E is the glucose content, and F is the sucrose content.

[0039] Biomaterial deposit information

[0040] The strain information deposited in this application is: Bacillus funiculosus Ectobacillus sp. strain B267, which is taxonomically designated as: Ectobacillus sp. B267, Chinese classification name: Bacillus funiculosus B267, preservation number: CCTCCNO: M2025411; the strain is deposited in the China Center for Type Culture Collection, address: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the preservation date is March 10, 2025. DETAILED DESCRIPTION

[0041] All features disclosed in this specification, or all steps in the disclosed methods or processes, except mutually exclusive features and / or steps, can be combined in any manner.

[0042] Any feature disclosed in this specification (including any accompanying claims and abstract), unless otherwise stated, is merely an example of a series of equivalent or similar features. Example 1

[0043] This example is the isolation and identification of Bacillus funiculosus B267.

[0044] 1. Isolation and purification of strain B267: It was isolated from the rhizosphere of pear trees with long-term potassium deficiency in Lishui District, Nanjing. Specifically, 1 g of rhizosphere soil was collected and added to a 50 ml Erlenmeyer flask containing glass beads and 9 mL of SM buffer. The suspension was shaken at 30°C and 170 rpm for 30 min to obtain a soil suspension. Sterile water was added to gradually dilute the suspension to 10 -5 ~10 -7 The suspension was spread on TSA medium and cultured at 30°C for 48 hours. Then, a single bacterium was picked out by the plate streak method to purify the strain.

[0045] 2. Identification of strain B267: ① Morphological identification: Figure 1As shown, strain B267 was inoculated onto TSA medium and cultured in a constant temperature incubator. After 24 hours of culture, round colonies appeared on the culture dish. They were yellow-white, opaque, and had regular edges. The morphological characteristics of Bacillus funiculosus were consistent with those of Bacillus funiculosus. Combined with molecular identification, the strain was confirmed to be Bacillus funiculosus. ② After DNA was extracted and sequenced, molecular identification was performed. The DNA sequence of the strain was amplified and the phylogenetic tree of strain B267 was analyzed and identified based on the DNA sequence (see Figure 2). Figure 2 ), confirming that the strain Ectobacillus sp. is closely related, as shown in the evolutionary tree. Figure 2 Combined with morphological identification, the strain was classified and named Ectobacillus sp. Example 2

[0046] This example is a growth-promoting function assay of strain B267.

[0047] 1. Qualitative determination of IAA production function of strains: add 1% inoculum of B267 strain to TSB medium containing L-tryptophan, 30℃, 170 rpm min -1 After shaking for 48 hours, centrifuge at 10,000 rpm for 5 minutes to obtain the supernatant. Take 100 μL of the supernatant and mix it with an equal volume of Salkowski colorimetric solution. Mix 100 μL of uninoculated culture medium with an equal volume of Salkowski colorimetric solution as a blank control. React in the dark for 30 minutes. If the solution turns red, it means that the strain has the ability to produce IAA.

[0048] 2. Determination of the strain's potassium-solubilizing ability: 10 μL of the activated B267 suspension was added to a silicate solid medium containing potassium feldspar as a potassium source. This was repeated three times. The culture was incubated at 28°C for 5 days. The culture medium was observed for the presence of halos or clear zones. The strain's ability to solubilize poorly soluble potassium was determined by the ratio of the halo or clear zone (D) to the colony diameter (d). Simultaneously, a 1% inoculum of the B267 strain was added to a silicate liquid medium containing potassium feldspar as a potassium source. This was repeated three times. The culture was shaken at 28°C for 2 days. The supernatant was then assayed for potassium ion content using a flame spectrophotometer.

[0049] The above growth-promoting properties are shown in Table 1 and Figure 3 shown.

[0050]

[0051] From Table 1 and Figure 3 It can be seen that strain B267 has the ability to produce IAA and significantly solubilize insoluble potassium. Example 3

[0052] This example studies the growth-promoting effect of strain B267 on Arabidopsis thaliana.

[0053] (1) Arabidopsis seedling cultivation: Arabidopsis seeds were placed in sterile water and soaked for 24 hours, then soaked in 75% alcohol for 30 seconds, rinsed 3-5 times with sterile water, soaked in 2% sodium hypochlorite for 5 minutes, and finally cleaned with sterile water. The sterilized seeds were spread on agar-only culture medium and placed in a 28°C constant temperature incubator for germination for 3-5 days. Germinated seeds were selected for transplanting.

[0054] (2) Study on the growth-promoting effect of B267 VOC (volatile organic compounds): The prepared B267 bacterial suspension (final concentration of 10 7 CFU / g soil) on one side of the partition plate, and three Arabidopsis seedlings were transplanted into the other side. The uninoculated treatment was used as the control. Each treatment was repeated 12 times. After culturing for 20 days, samples were collected and the phenotypic traits of Arabidopsis were determined.

[0055] (3) Study on the growth-promoting effect of B267 secretion: B267 was inoculated into TSB medium and centrifuged at 5000 g after 24 hours to extract the supernatant. The supernatant was rotary evaporated in units of 1 ml and gradiently diluted 10 2 , 10 3 and 10 4 In the culture medium, 5 Arabidopsis seedlings were transplanted into the culture medium at the same time, and no fermentation liquid was added as a control treatment. Each treatment was repeated 12 times, and samples were collected after culturing for 20 days to determine the phenotypic traits of Arabidopsis.

[0056] Test results: 1. Figure 4 As shown in Figure 2, VOCs from B267 can significantly promote the growth of Arabidopsis seedlings. Figure 5 As shown, compared with the control group, the root dry weight, aboveground dry weight and total dry weight of Arabidopsis seedlings inoculated with strain B267 were significantly increased by 508.00%, 323.49% and 337.06%, respectively.

[0057] 2. If Figure 6 As shown in Figure 2, VOCs from strain B267 can improve the root morphology of Arabidopsis thaliana. Figure 7 As shown, compared with the control group, the root length, root surface area and root tip number of Arabidopsis seedlings inoculated with strain B267 increased by 142.15%, 182.29% and 56.78%, respectively.

[0058] 3. If Figure 8 As shown in Figure 2, different concentrations of the secretion of strain B267 can significantly promote the growth and root morphology of Arabidopsis seedlings; Figure 9 and Figure 10As shown in the results, compared with the control, the root dry weight, aboveground dry weight and total dry weight of Arabidopsis seedlings treated with different concentrations of the secretion of strain B267 were significantly increased, and the root length, root surface area and root tip number were also significantly increased; 2 , 10 3 and 10 4 The effects of the secretions on the dry weight of Arabidopsis seedling roots were not significant. 2 The secretion of 10 2 and 10 3 The effect of secretions on the total dry weight of Arabidopsis seedlings was significantly higher than that of other concentrations. Example 4

[0059] This example studies the growth-promoting effect of strain B267 on pear and tomato seedlings.

[0060] (1) Tomato and pear seedling cultivation: Soak the seeds in sterile water for 24 hours, then soak them in 75% alcohol for 30 seconds, rinse them with sterile water for 3-5 times, add 2% sodium hypochlorite and soak them for 20 minutes, and finally rinse them with sterile water. Spread the sterilized seeds on a culture medium lined with filter paper soaked in sterile water, place them in a 28°C constant temperature incubator for germination for 3-5 days, select the germinated seeds and sow them in a sterilized substrate for seedling cultivation. When the seedlings in the substrate have 2-3 true leaves, select healthy and uniform seedlings and transplant them into sterilized substrate pots.

[0061] (2) Inoculation: Inoculate the prepared B267 bacterial suspension (final concentration of 10 7 CFU / g soil) was inoculated into the rhizosphere of seedlings, and non-inoculated treatment was used as the control. Each treatment had 12 replicates. After 60 days of culture, samples were collected and the phenotypic traits of tomato and pear were determined.

[0062] (3) K + Flow rate measurement: Select plants with consistent growth and good condition within the group. Use filter paper strips and resin blocks to fix the complete roots to the bottom of the culture dish, exposing the root tip. Add the test solution (0.05mM KCl, 0.2mM MES, pH 5.8) to the culture dish to immerse the roots. After 30 minutes, add the sample for testing. Under a microscope, find the root site to be tested (a point on the root surface 1200μm from the root tip, which is the elongation zone) and place the KCl solution on the root surface. + The flow rate microsensor was placed 5 μm away from the test site without contacting the sample, and the test was started. Data was recorded for 5 minutes for each sample, and 6 biological replicates were tested in each group. K was directly read and output using imFluxes V3.0 software. + Flow rate data, flow rate unit is pico mol • cm -2 • s -1 The positive and negative values ​​of flow rate only represent K+ Transfer direction, negative value indicates K + Flows from outside the root cells into the root cells; positive value is the opposite.

[0063] Test results: 1. Figure 11 As shown in Figure 2, B267 can significantly promote the growth of tomato seedlings; Figure 12 As shown in the figure, compared with the control, although the root dry weight of tomato seedlings inoculated with B267 did not reach a significant difference, the aboveground dry weight and total dry weight were significantly increased by 13.61% and 12.80%, respectively.

[0064] 2. If Figure 13 As shown in Figure 2, inoculation with B267 significantly improved the root morphology of tomatoes. Figure 14 As shown, compared with the control group, the root length, root surface area and root tip number of tomato increased by 13.21%, 20.71% and 37.17%, respectively.

[0065] 3. If Figure 15 As shown in Figure 2, inoculation with B267 significantly promoted the growth of Pyrus betulae seedlings. Figure 16 As shown in the results, compared with the control group, the root dry weight, aboveground dry weight and total dry weight of Pyrus betulaceae seedlings inoculated with B267 were significantly increased by 65.32%, 54.61% and 56.64%, respectively.

[0066] 4. If Figure 17 As shown in Figure 2, inoculation with B267 can improve the root morphology of Pyrus betulaeformis. Figure 18 As shown, compared with the control group, the root length, root surface area and root tip number of the B267 inoculated treatment increased by 49.11%, 65.23% and 116.01%, respectively.

[0067] 5. If Figure 19 As shown in the figure, inoculation with B267 could significantly increase the root K + Absorption rate 2806.41%. Example 5

[0068] This example studies the effect of strain B267 on tomato plant yield and fruit quality.

[0069] (1) Tomato seedling cultivation: Soak MicroTom tomato seeds in sterile water for 24 hours, then soak them in 75% alcohol for 30 seconds, rinse them with sterile water for 3-5 times, add 2% sodium hypochlorite and soak them for 20 minutes, and finally rinse them with sterile water. Spread the sterilized seeds on a culture medium lined with filter paper soaked in sterile water, place them in a 28°C constant temperature incubator for germination for 3-5 days, select the germinated seeds and sow them in a sterilized substrate for seedling cultivation. When the seedlings in the substrate have 2-3 true leaves, select healthy and uniform seedlings and transplant them into sterilized substrate pots.

[0070] (2) Inoculation: When the tomato plants enter the flowering stage, inoculate the prepared B267 bacterial suspension (final concentration of 10 7 CFU / g soil) were inoculated into the rhizosphere, with an uninoculated control treatment. Each treatment had 12 replicates, and flower marking was performed every two days for a total of three times. After 45 days of incubation, samples were collected to calculate yield and determine tomato fruit traits.

[0071] (3) Determination of tomato fruit quality indicators: For the soluble solid content, 10 fruits that had changed color after the second flower marking were uniformly selected and juiced, and the juice was determined by refractometer. For the content of three soluble sugars (fructose, glucose, and sucrose), 1 g of fresh fruit sample was added to 50 mL of water, vortexed for 10 min, ultrasonicated for 30 min, and centrifuged at 9000 r / min for 10 min. The supernatant was removed and coarsely filtered through qualitative rapid filter paper, diluted, and passed through a 0.22 μm aqueous syringe filter to obtain the sample to be tested. Soluble sugars were quantitatively analyzed using an ion chromatograph. For the vitamin C content, 2,6-dichloroindophenol titration was used for determination. Weigh 10 g of fresh sample, add about 5 ml of 2% oxalic acid solution in a mortar and grind it. Transfer it to a 100 ml volumetric flask, make up to volume with 2% oxalic acid, and centrifuge it in a high-speed low-temperature centrifuge. After centrifugation, take 10 ml of the supernatant and place it in a conical flask. Titrate it with standardized 2,6-dichlorophenol indophenol solution until it turns pink and does not fade within 30 seconds.

[0072] Test results: 1. Figure 20 As shown in the figure, after inoculation with B267 bacterial suspension, the tomato fruits were significantly larger than the CK control group without bacterial suspension inoculation, indicating that B267 bacterial suspension can significantly increase tomato fruit yield.

[0073] 2. If Figure 21 As shown in the figure, compared with the control, although the soluble solids, fructose and glucose contents of tomato fruit did not change significantly after inoculation with B267 bacterial suspension, the Vc and sucrose contents increased significantly while the yield increased by 12.79%.

[0074] In summary, the funicular Bacillus B267 screened by the applicants of the present invention has good IAA production and potassium solubilization capabilities. Plate tests found that the volatile organic compounds and secretions produced by it can effectively promote the development of the root system and aboveground parts of Arabidopsis thaliana. Potted plant experiments found that B267 can significantly improve the root development morphology of tomato and pear seedlings, and enhance the root system's K absorption. + The absorption rate of B267 strain can increase the biomass, yield and quality of seedlings, and has a good growth-promoting effect on plants. After inoculation with B267 strain, it can replace chemical potassium fertilizer to a certain extent and reduce fertilizer input. This shows that the strain of this application can be promoted and utilized as a biological organic fertilizer material.

[0075] The above-described embodiments merely illustrate several embodiments of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make various modifications and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. Bacillus funiculosus Ectobacillus sp. strain B267 or containing Bacillus funiculosus Ectobacillus Application of a fungicide of sp. strain B267 in promoting plant growth and improving tomato fruit quality; the plants are Arabidopsis thaliana, tomato and Pyrus betula; The tomato fruit quality includes: tomato fruit size, fruit yield, fruit vitamin C content and fruit sucrose content; Bacillus funiculosus Ectobacillus sp. strain B267 is deposited with CCTCC NO: M2025411.

2. The funicular Bacillus according to claim 1 Ectobacillus sp. strain B267 or comprising the funicular Bacillus as claimed in claim 1 Ectobacillus Application of bacterial agent of sp. strain B267 in preparation of biological organic fertilizer.

3. The funicular Bacillus according to claim 1 Ectobacillus sp. strain B267 or comprising the funicular Bacillus as claimed in claim 1 Ectobacillus Application of a bacterial agent of sp. strain B267 in producing IAA and / or potassium solution.

4. The funicular Bacillus according to claim 1 Ectobacillus sp. strain B267 or comprising the funicular Bacillus as claimed in claim 1 Ectobacillus Application of bacterial agent derived from sp. strain B267 in reducing the use of chemical fertilizers.

5. Use of the funicular Bacillus as claimed in claim 1 Ectobacillus sp. strain B267 or comprising the funicular Bacillus as claimed in claim 1 Ectobacillus sp. strain B267 bacterial agent to promote plant growth, characterized in that, The method comprises the following steps: Ectobacillus The bacterial suspension and / or fermentation liquid of sp. strain B267 is poured into the rhizosphere of the plant seedlings.

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