Sound field evaluation system and evaluation method
Through the sound field evaluation system, fluorescence intensity values are detected using a fluorescence spectrophotometer, which solves the problem of complex and costly determination of ultrasonic sound field distribution, and achieves high-precision and low-cost sound field distribution testing.
Patent Information
- Application Number
- CN202510611651.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-13
- Publication Date
- 2025-07-08
AI Technical Summary
In the prior art, the method of determining ultrasonic sound field distribution is complex and costly, requiring multiple professionals to participate, the operation is difficult and the result error is large, so it is impossible to obtain experimental data efficiently and at low cost.
The sound field evaluation system of the cuvette unit, ultrasonic unit and evaluation unit is adopted to adjust the relative position of the ultrasonic transducer and the cuvette, and use a fluorescence spectrophotometer to detect the fluorescence intensity value, so as to achieve simple and intuitive evaluation of the sound field distribution.
It realizes high-precision, low-cost, and low-operation difficulty sound field distribution testing, can quickly obtain experimental results, and solves the problems of complex calculations and high-cost in the existing technology.
Smart Images
Figure CN120274870A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of ultrasonic transducer evaluation, and particularly relates to an acoustic field evaluation system and an evaluation method. Background Art
[0002] In recent decades, ultrasound has been widely applied in medicine, industry, and scientific research. Efficiently utilizing the distribution of the ultrasonic acoustic field is crucial for fields such as medicine and industry. In the prior art, the determination of the ultrasonic acoustic field distribution usually involves complex calculations using formulas or is determined based on high-cost equipment such as hydrophones. The former requires multiple complex parameters, and it is difficult to directly measure some variable parameters. Multiple formulas also need to be applied for solution. If the error of a certain parameter is relatively large during the measurement process, it will affect the final calculation result. The latter has a high construction cost and is not applicable to the determination of the ultrasonic acoustic field in every field. Moreover, both of the above methods require the joint participation of multiple professionals, and the requirements for operators are also very high. This results in difficulties in getting started, as well as problems of low work efficiency and large result errors. Summary of the Invention
[0003] To solve the above technical problems, in a first aspect of the present invention, an acoustic field evaluation system is provided, including:
[0004] A cuvette unit, including a cuvette and a fluorescence spectrophotometer. There is a macrophage suspension pre-incubated with a photosensitizer and a reactive oxygen species probe in the cuvette;
[0005] An ultrasonic unit, including an ultrasonic transducer and an adjusting device. The ultrasonic transducer adjusts its relative position with the cuvette through the adjusting device. When the ultrasonic transducer works, reactive oxygen species are generated in the macrophages in the cuvette and combine with the reactive oxygen species probe. The fluorescence spectrophotometer excites the reactive oxygen species probe, receives the emitted light signal, and generates a fluorescence intensity value;
[0006] An evaluation unit, used to obtain the fluorescence intensity values generated by the fluorescence spectrophotometer when the ultrasonic transducer and the cuvette are in different relative positions, and obtain an evaluation result based on the fluorescence intensity values collected at all relative positions.
[0007] Further, a telescopic liquid collector is provided between the cuvette and the ultrasonic transducer, and the telescopic liquid collector is filled with medium water or an ultrasonic coupling agent.
[0008] Further, the telescopic liquid collector has an open upper end and a closed lower end and is a hollow structure inside. The side wall of the telescopic liquid collector includes a first side surface and a second side surface arranged oppositely. The first side surface and the second side surface are connected by a telescopic side wall. One side of the cuvette is installed on the first side surface, and the transducer bracket is fixedly connected to the second side surface.
[0009] Further, the telescopic side wall is formed by sequentially connecting a plurality of foldable corrugated sheets.
[0010] Further, a first avoidance hole is provided on the first side surface, and a second avoidance hole is provided on the second side surface. The first avoidance hole corresponds to the shape and size of the liquid area in the cuvette, and the second avoidance hole corresponds to the shape and size of the ultrasonic transducer.
[0011] Further, the adjusting device includes three groups of adjusting components. The adjusting directions of the three groups of adjusting components are perpendicular to each other in three-dimensional space, and a water baffle is provided on the adjusting component.
[0012] The second aspect of the present invention provides a sound field evaluation method, which is implemented based on the sound field evaluation system, and includes:
[0013] The adjusting component drives the ultrasonic transducer to move to a specified position, adjusts the ultrasonic parameters, and starts the ultrasonic process;
[0014] During the ultrasonic process, the ultrasonic transducer stimulates macrophages in the cuvette to generate reactive oxygen species, which combine with the fluorescent probe. The fluorescence spectrophotometer excites the reactive oxygen species probe and receives the emitted light signal to obtain the fluorescence intensity value.
[0015] Compared with the prior art, the present invention has the following technical effects:
[0016] The detection system built by the present invention adjusts the relative position between the ultrasonic transducer and the cuvette, and according to the fluorescence spectrophotometer, obtains the fluorescence intensity values of the cuvette at different sound field positions, and then determines the optimal area of the ultrasonic transducer. During the test, no complex control program and sound field algorithm are required. Only the position of the ultrasonic transducer needs to be adjusted semi-automatically. Through a limited number of experiments, the production of reactive oxygen species can be monitored in real time to directly obtain the experimental results. The present invention has high detection accuracy, simple test process, low operation difficulty, and low cost. It can intuitively and efficiently complete the test of the sound field distribution, and solves the two major problems that single medicine cannot obtain the desired experimental data and single engineering measurement has a long time-consuming and complex calculation. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 It is a schematic structural diagram of the sound field evaluation system according to a specific embodiment of the present invention;
[0018] Figure 2 It is a schematic overall structure diagram of the sound field evaluation system according to a specific embodiment of the present invention;
[0019] Figure 3 It is a schematic structural diagram of the adjusting device according to a specific embodiment of the present invention;
[0020] Figure 4 It is a schematic structural diagram of another perspective of the adjusting device according to a specific embodiment of the present invention;
[0021] Figure 5 It is a schematic structural diagram of a telescopic liquid collector according to a specific embodiment of the present invention;
[0022] Figure 6 It is a schematic structural diagram among a transducer bracket, a telescopic liquid collector and a cuvette according to a specific embodiment of the present invention
[0023] Figure 7 It is a schematic flow diagram of a sound field evaluation method according to a specific embodiment of the present invention;
[0024] Figure 8 They are fluorescence intensity values at each position point under different sound field intensities in a specific embodiment of the present invention. Detailed implementation manners
[0025] The following uses specific specific examples to illustrate the implementation manners of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation manners. Various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that, without conflict, the following embodiments and the features in the embodiments can be combined with each other.
[0026] It should be noted that the diagrams provided in the following embodiments only illustrate the basic concept of the present invention in a schematic manner. Therefore, only the components related to the present invention are shown in the diagrams, rather than being drawn according to the number, shape and size of the components in actual implementation. The type, quantity and proportion of each component in actual implementation can be an arbitrary change, and the component layout type may also be more complex.
[0027] For the purpose of illustration, some exemplary embodiments of the present invention are described. It should be understood that the present invention can be implemented in other ways not specifically shown in the drawings.
[0028] In a specific embodiment, a sound field evaluation system is provided, as Figure 1 shown, including a cuvette 3 unit, an ultrasonic unit and an evaluation unit. By changing the relative position between the ultrasonic unit and the cuvette 3 unit, different fluorescence intensity values are obtained, and the evaluation unit determines the optimal action area of the ultrasonic unit according to the change of the ultrasonic unit position and the fluorescence intensity.
[0029] It was experimentally found that after mouse bone marrow primary macrophages phagocytosed the sonosensitizer, while keeping parameters such as the volume of the cell suspension, the environmental temperature, and the cell state unchanged, under the action of ultrasonic waves with set parameters, reactive oxygen species would be produced in the macrophages. The production of reactive oxygen species under different ultrasonic intensities was detected using a reactive oxygen species probe, and it was obtained that there was a corresponding linear relationship between the ultrasonic intensity and the production of reactive oxygen species under the ultrasonic parameters. The production of reactive oxygen species could be objectively reflected through the fluorescence intensity value. Based on this, this application uses the fluorescence intensity value to reflect the ultrasonic intensity at a certain point or a small area within the sound field.
[0030] As Figures 2-4 shown, in this embodiment, the cuvette 3 unit includes a cuvette 3, a fluorescence spectrophotometer, and a cuvette fixing plate 4. The cuvette 3 is installed on the cuvette fixing plate 4; the cuvette 3 contains PBS liquid (phosphate buffer solution, maintaining the cell osmotic pressure) and cells (the cells contain pre-incubated sonosensitizer and reactive oxygen species probe). The ultrasonic waves emitted by the ultrasonic transducer 1 will activate the sonosensitizer in the cells to produce reactive oxygen species, and the reactive oxygen species oxidize the probe, generating fluorescence that can be detected by the fluorescence spectrophotometer. The reactive oxygen species probe used in this embodiment is DCFH-DA (2',7'-dichlorofluorescein diacetate), and the level of reactive oxygen species (ROS) in the cells is quantified by detecting the fluorescence intensity of its oxidation product DCF (dichlorofluorescein). DCFH-DA itself has no fluorescence and can freely pass through the cell membrane and enter the cells. Inside the cells, DCFH-DA is hydrolyzed by esterase to generate DCFH, which is then oxidized by the reactive oxygen species generated by ultrasonic excitation of the sonosensitizer to generate fluorescent DCF. The fluorescence intensity of DCF is proportional to the level of reactive oxygen species in the cells, so the level of reactive oxygen species in the cells can be reflected by detecting the fluorescence intensity of DCF. During the test, the ultrasonic parameters are set, and under the action of ultrasonic waves with the set parameters, reactive oxygen species are produced in the macrophages and combined with the fluorescence probe, and the cells remain in a viable state throughout the process.
[0031] Since the fluorescence is very weak and a dark environment free from light is required, the ultrasonic transducer 1 unit and the cuvette 3 unit are integrated in a sealed housing, and the dark environment formed by the sealed housing can meet the requirements of the fluorescence spectrophotometer for the light environment.
[0032] The fluorescence spectrophotometer in this embodiment includes an excitation light source and an emission light receiving detector. The excitation light source and the emission light receiving detector are respectively fixed on the inner wall of the housing. The excitation light emitted by the excitation light source passes through the cuvette 3, and the emission spectrum generated by exciting the probe is received by the emission light receiving detector and generates a fluorescence intensity value. In this embodiment, the fluorescence spectrophotometer is a finished product device in the prior art and is installed at the corresponding position in the housing according to the position of the cuvette 3.
[0033] Due to the considerations of the optical path, the excitation light source and the emission light receiving detector in the cuvette 3 unit need to be fixed on the inner wall of the housing. The sound field generated by the ultrasonic transducer 1 is a three-dimensional sound field. In order to detect the biological effects that can be achieved at each position point or any area in this three-dimensional sound field and comprehensively evaluate the sound field of the ultrasonic transducer 1, as shown in the figure, the ultrasonic unit in this embodiment includes an ultrasonic transducer 1, a transducer support 2, and an adjustment device. The ultrasonic transducer 1 is fixed on the transducer support 2, and the transducer support 2 is installed on the adjustment device. The adjustment device drives the ultrasonic transducer 1 to move in three-dimensional space, changing the relative position between the ultrasonic transducer 1 and the cuvette 3. The relative position between the ultrasonic transducer 1 and the cuvette 3 is adjusted by the adjustment device, so that the cuvette 3 is located at different sound field points, resulting in different yields of reactive oxygen species in the cuvette 3, and thus the area with the best sonodynamic effect can be determined. When the ultrasonic transducer 1 works, reactive oxygen species are generated in the macrophages in the cuvette 3 and combine with the reactive oxygen species probe. The fluorescence spectrophotometer excites the reactive oxygen species probe, receives the emission light signal, and generates a fluorescence intensity value.
[0034] The adjustment device includes a first adjustment component, a second adjustment component, and a third adjustment component. The first adjustment component is used to adjust the vertical distance between the ultrasonic transducer 1 and the cuvette 3. The adjustment directions of the second adjustment component and the third adjustment component drive the first adjustment group to move in the horizontal direction and the vertical direction respectively. The adjustment directions of the three adjustment components are perpendicular to each other in three-dimensional space, so as to adjust the position of the ultrasonic transducer in three-dimensional space. As Figure 5 shown, the first adjustment component includes a first drive motor 6, a first lead screw 7, and a first slider 8 installed on the first lead screw 7. The ultrasonic transducer 1 is fixedly installed on the first slider 8 through the transducer support 2. The extending direction of the first lead screw 7 is defined as the z-axis direction. The first drive motor 6 drives the first slider 8 to reciprocate through the first lead screw 7, realizing the adjustment of the distance between the ultrasonic transducer 1 and the cuvette 3 in the z direction.
[0035] The second adjustment component includes a second drive motor 9, a second lead screw 10, and a second slider 11 installed on the second lead screw 10. The first adjustment component is fixed on the second slider 11. The second lead screw 10 is arranged in the vertical direction. The extending direction of the second lead screw 10 is defined as the y-axis direction. The second drive motor 9 drives the second slider 11 to reciprocate through the second lead screw 10, thereby realizing the adjustment of the ultrasonic transducer 1 in the y-axis direction, that is, adjusting upward or downward in the vertical direction.
[0036] The third adjustment component includes a third driving motor 12, a third lead screw 13, and a third slider 14 mounted on the third lead screw 13. The second adjustment component is fixed to the third slider 14. The third lead screw 13 is arranged in a horizontal direction. The extension direction of the third lead screw 13 is defined as the x-axis direction. The third driving motor 12 drives the third slider 14 to reciprocate through the third lead screw 13, thereby realizing the adjustment of the ultrasonic transducer 1 in the x-axis direction, that is, adjusting left or right in the horizontal direction.
[0037] The first adjustment component, the second adjustment component, and the third adjustment component cooperate to enable the ultrasonic transducer 1 to move in a three-dimensional space. In this embodiment, the lead screw precision of each driving motor is selected to be 0.1 mm. During the movement, the minimum step distance achieved by each lead screw is 0.1 mm, which can achieve millimeter-level movement and can obtain a more accurate evaluation result. During use, the precision of the driving motor and the lead screw can be adjusted according to needs to meet different precision requirements. The three groups of adjustment components can achieve the movement of the ultrasonic transducer 1 at any position point (x i , y i , z i) . The three groups of adjustment components enable the ultrasonic transducer 1 to move in a three-dimensional space, and thus an action area composed of each position point can be obtained. The evaluation unit screens out the effective area within the action area.
[0038] The evaluation unit is used to collect the fluorescence intensity values of the ultrasonic transducer 1 at different position points during ultrasonic action, sort the fluorescence intensity values at each position point in descending order, and select the position points corresponding to the top n fluorescence intensity values according to application needs. The area composed of the n position points is the area with the best reactive oxygen species production rate, that is, the area with the best sonodynamic effect, and this area will become the effective area for clinical treatment.
[0039] When evaluating multiple ultrasonic transducers 1, the evaluation unit can evaluate the ultrasonic transducers 1 respectively in the above manner. For ultrasonic transducers with low precision requirements or similar application requirements, a fluorescence intensity threshold can be set, and the area composed of all position points greater than the fluorescence intensity threshold is used as the best area.
[0040] After determining the best area, the ultrasonic transducers 1 are sorted or graded according to the size of the best area and applied in different fields.
[0041] The ultrasonic transducer 1 is used to eliminate plaques in subcutaneous blood vessels. During the treatment, when the ultrasonic transducer 1 located outside the body and the ultrasonic transducer 1 perform sonodynamic therapy, the transducer is attached to the skin surface, and the blood vessels are located under the skin. There is a certain distance between the head end of the transducer and the arterial plaque, and the sound field transmission weakens, affecting the final measurement result. To solve this problem, the evaluation system further includes a liquid collector unit, as Figure 5 shown. It includes a telescopic liquid collector 5 and a liquid collector fixing plate 15. The telescopic liquid collector has an open upper end, a closed lower end, and a hollow internal structure. The side wall of the telescopic liquid collector includes a first side 51 and a second side 52 arranged opposite to each other. The telescopic liquid collector 5 is located between the ultrasonic transducer 1 and the cuvette 3. When it is necessary to measure the experimental data in the direction away from the cuvette 3 by the transducer, that is, the direction in which the transducer is far from the cuvette 3, degassed pure water or ultrasonic coupling agent can be injected into the water storage device to ensure the accuracy of the experimental data, and then the ultrasonic field intensity when the ultrasound of the ultrasonic transducer 1 passes through tissues such as the skin and fat and reaches the plaque can be simulated.
[0042] During the movement of the ultrasonic transducer 1, the volume of the telescopic liquid collector 5 often changes. Since the upper end of the telescopic liquid collector 5 has an open structure, in order to prevent the medium water in the liquid collector from spilling out and to enable the repeated use of the telescopic liquid collector 5, the first side 51 and the second side 52 in this embodiment are connected by a telescopic side wall. The telescopic side wall is formed by sequentially connecting a plurality of foldable corrugated sheets 54. As shown in the figure, the foldable corrugated sheet 54 in this embodiment is generally U-shaped. The sequentially connected foldable corrugated sheets 54 enable the telescopic liquid collector 5 to be stretched in any direction. When the foldable corrugated sheet 54 is folded, the volume of the telescopic liquid collector 5 can be reduced. After testing, the telescopic liquid collector 5 can still return to its original shape, saving the test cost. In this embodiment, the telescopic liquid collector 5 is made of a soft rubber material with a hardness requirement of 30 to ensure its stretchability during use. It is prepared by 3D printing technology and has the advantages of low cost, high efficiency, and high precision.
[0043] The first side 51 of the telescopic liquid collector 5 is fixed on the liquid collector fixing plate 15, and the second side 52 is fixed on the transducer support. Through grooves 16 that match the shape and size of the liquid area in the cuvette 3 are provided on both the liquid collector fixing plate 15 and the cuvette fixing plate 4. The cuvette 3 is located between the liquid collector fixing plate 15 and the cuvette fixing plate 4, and the through grooves are respectively located on both sides of the cuvette 3, so that the ultrasonic transducer 1 will not be blocked or reflected during operation, affecting the test results. A first avoidance hole 55 is provided on the first side of the telescopic liquid collector 5, and a second avoidance hole 56 is provided on the second side 52. The first avoidance hole 55 matches the shape and size of the working area of the cuvette 3, and the second avoidance hole 56 matches the shape and size of the probe of the ultrasonic transducer 1. During operation, the two avoidance holes of the telescopic liquid collector 5 are sealed respectively through the ultrasonic transducer 1 and the cuvette 3.
[0044] In this embodiment, for safety considerations, water shields are provided on the surfaces of the first drive motor, the second drive motor, and the third drive motor to prevent the liquid in the telescopic liquid collector from spilling out and damaging the equipment.
[0045] As Figure 6 shown, the liquid collector fixing plate 15 extends into the telescopic liquid collector 5 from top to bottom and is closely arranged against the inner wall of the first side 51. An installation groove 53 that matches the external shape and size of the cuvette 3 is provided on the outside of the first side 51 of the liquid collector. One side of the cuvette 3 is installed in the installation groove 53, and the other end of the cuvette 3 abuts against the cuvette fixing plate 4. A support plate 41 is provided on the cuvette fixing plate 4 to further support the cuvette 3 and improve the stability of the device.
[0046] The transducer support 2 includes a first fixing plate 21 and a second fixing plate 22. The first fixing plate 21 and the second fixing plate 22 respectively fix both sides of the ultrasonic transducer 1. The first fixing plate 21 extends into the telescopic liquid collector 5 from top to bottom and is closely attached to the inner wall of the second side 52. The liquid collector fixing plate 15 and the first fixing plate 21 can not only fix the telescopic liquid collector 5, but also the two plates can be respectively located on the two sides, keeping the shapes of both sides of the telescopic liquid collector 5 fixed. The forces on both sides are evenly distributed, enabling the telescopic liquid collector 5 to freely expand and contract, preventing damage to the side walls of the telescopic liquid collector 5, and realizing the reuse of the telescopic liquid collector 5. During the movement of the ultrasonic transducer 1, the relative position between its head and the telescopic liquid collector 5 remains unchanged, and the accuracy of detection can also be ensured.
[0047] Based on the evaluation system described in the above embodiment, in a specific embodiment, an evaluation method is provided. As Figure 7 shown,
[0048] S1. Set parameters, where the parameters include the expected number of measurement points, sequence, moving step of the ultrasonic transducer, moving direction, ultrasonic activation component, etc.;
[0049] S2. The adjustment component drives the ultrasonic transducer 1 to move to the specified measurement point according to the expected set parameters, adjusts the ultrasonic parameters, and starts the ultrasonic process; during the ultrasonic process, the ultrasonic waves stimulate the macrophages in the cuvette 3 to produce reactive oxygen species, which combine with the fluorescent probe. The fluorescence spectrophotometer excites the reactive oxygen species probe and receives the emitted light signal to obtain the fluorescence intensity value.
[0050] S3. Determine whether this point is the preset last measurement point. If so, output the fluorescence intensity values of all measurement points, and then obtain the sound field distribution of each position point. If not, repeat step S2 until all measurement points are traversed.
[0051] To further illustrate the specific effects of the present application, in a specific embodiment, using primary mouse bone marrow macrophages and protoporphyrin IX as a photosensitizer, the influence of the ultrasonic transducer 1 at different positions on the reactive oxygen species production rate under specific intensities was detected by the sound field evaluation system of the above embodiment. The selected cuvette 3 has a standard volume size, with a length * width * height of 1 * 1 * 4 cm, and the liquid in the telescopic liquid collector 5 is 3 ml. The measurement method is as follows: Under the same ultrasonic parameters, keep the positions of the first adjustment component and the second adjustment component unchanged, and only adjust the third adjustment component. Place the ultrasonic transducer 1 at the right edge of the cuvette 3 and in contact with the cuvette 3. At the start of the measurement, the right edge of the ultrasonic transducer 1 is in contact with the cuvette 3 (SDT - 4 mm), and it moves to the right at a step of 2 mm each time until the left edge of the ultrasonic transducer 1 is disengaged from the cuvette 3. The influence results of the sound fields of the ultrasonic transducer 1 at different positions on the production of reactive oxygen species by macrophages under different ultrasonic intensities (0.3 / 0.5 / 0.7 / 0.9 / 1.1 / 1.3 W / cm 2 ) are as follows Figure 8As shown in the figure, the abscissa SDT-XXmm in the figure represents the position of the probe of the ultrasonic transducer 1, and the ordinate represents the level of reactive oxygen species generated after applying ultrasound. The vertical dotted line in the figure marks the central position of the ultrasonic transducer 1. Among them, control represents the control group, pure macrophages; PpIX represents the protoporphyrin IX group, in which cells are incubated with protoporphyrin IX but no ultrasonic intervention is applied; Ultrasound represents the pure ultrasound group, where the ultrasonic transducer 1 is at the central position and ultrasonic intervention of corresponding intensity is applied, but the macrophages are not incubated with protoporphyrin IX. The position change range of the ultrasonic transducer 1 is from SDT-4mm to SDT-28mm, with a step size of 2mm, moving from left to right, and SDT-16mm is the central position of the ultrasonic transducer 1. It can be seen from the figure that when the ultrasonic transducer 1 is at the same position, the yield of reactive oxygen species depends on the ultrasonic intensity, that is, the greater the ultrasonic intensity, the higher the yield of reactive oxygen species. At each ultrasonic intensity, the yield of reactive oxygen species is the highest at the central position of the ultrasonic transducer 1, and the sound field at the two edge positions on both sides of the transducer has little effect on the yield of reactive oxygen species, which is basically consistent with the sound field distribution detected by the hydrophone.
[0052] The above embodiments merely illustrate the principles and effects of the present invention, rather than limiting the present invention. Any person familiar with this technology can modify or change the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes completed by those with ordinary knowledge in the technical field without departing from the spirit and technical idea disclosed by the present invention should still be covered by the claims of the present invention.
Claims
1. An acoustic field evaluation system, characterized in that, Comprising: A cuvette unit, including a cuvette and a fluorescence spectrophotometer, wherein there is a macrophage suspension pre-incubated with a sonosensitizer and a reactive oxygen species probe in the cuvette; An ultrasonic unit, including an ultrasonic transducer and an adjustment device, wherein the relative position of the ultrasonic transducer with respect to the cuvette is adjusted by the adjustment device; An evaluation unit, configured to obtain the fluorescence intensity values generated by the fluorescence spectrophotometer at different relative positions of the ultrasonic transducer and the cuvette, and obtain an evaluation result based on the fluorescence intensity values collected at all relative positions.
2. The acoustic field evaluation system according to claim 1, characterized in that A telescopic liquid collector is provided between the cuvette and the ultrasonic transducer, and the telescopic liquid collector is filled with medium water or an ultrasonic coupling agent.
3. The sound field evaluation system according to claim 2, characterized in that, The telescopic liquid collector has an open upper end and a closed lower end and a hollow internal structure. The side wall of the telescopic liquid collector includes a first side face and a second side face arranged oppositely. The first side face and the second side face are connected by a telescopic side wall. One side of the cuvette is mounted on the first side face, and the transducer bracket is fixedly connected to the second side face.
4. The sound field evaluation system according to claim 3, wherein The telescopic side wall is formed by sequentially connecting a plurality of foldable corrugated sheets.
5. The sound field evaluation system according to claim 3, characterized in that, A first avoidance hole is formed on the first side face, and a second avoidance hole is formed on the second side face. The first avoidance hole matches the shape and size of the liquid area in the cuvette, and the second avoidance hole matches the shape and size of the ultrasonic transducer.
6. The sound field evaluation system according to claim 1, characterized in that, The adjustment device includes three groups of adjustment components, and the adjustment directions of the three groups of adjustment components are perpendicular to each other in three-dimensional space.
7. The sound field evaluation system according to claim 1, wherein The cuvette unit and the ultrasonic unit are installed in a sealed housing.
8. A method for sound field evaluation, characterized in that, Implemented based on the sound field evaluation system according to any one of claims 1-7, including: The adjustment component drives the ultrasonic transducer to move to a specified point, adjusts the ultrasonic parameters, and starts the ultrasonic process; During the ultrasonic process, the ultrasonic wave stimulates the macrophages in the cuvette to generate reactive oxygen species and combine with the fluorescence probe. The fluorescence spectrophotometer excites the reactive oxygen species probe and receives the emitted light signal to obtain the fluorescence intensity value; Repeat the above steps until the ultrasonic transducer traverses all preset points, obtain the fluorescence intensity values at all points, and output an evaluation result according to the fluorescence intensity values.