Mango embryo-promoting agent and embryo-promoting method

Abstract

The present invention provides a mango embryo-promoting agent and an embryo-promoting method, belonging to the field of plant planting technology. The mango embryo-promoting agent includes an inflorescence development stage embryo-promoting solution I; the formula of the inflorescence development stage embryo-promoting solution I includes: MS culture solution, AVG, GA3, imidacloprid, spinetoram, 6-BA, and KH2PO4; the mango embryo-promoting method comprises applying the mango embryo-promoting agent during the mango planting process to promote mango embryo development. The mango embryo-promoting agent and the embryo-promoting method of the present invention can reduce the proportion of mango embryoless fruits to about 80%, a reduction of about 10%.

Description

Technical Field

[0001] The invention belongs to the technical field of plant planting, and in particular relates to a mango embryo-promoting agent and an embryo-promoting method. Background Art

[0002] Mangoes are rich in carbohydrates, protein, fatty acids, vitamins, organic acids, dietary fiber, polyphenols, and carotenoids. Regular consumption can prevent obesity, diabetes, hypertension, and cancer, and possesses high economic and nutritional value. my country is the world's second-largest mango producer. In 2020, mango cultivation reached 5.13 million mu (approximately 1.1 million hectares), producing 2.67 million tons and generating 17 billion yuan in fresh fruit value. Across the country, 1.34 million households and 5.34 million people are employed in mango-related industries.

[0003] Currently, my country's main mango varieties, such as "Tainong No. 1," "Golden Glory," and "Kate," produce both embryoless and embryonic fruits. Embryonic fruits weigh approximately three times as much as those without. Naturally, about 90% of mangoes on each tree are embryoless. The standard for commercial mango production is a single fruit weight of at least 100 grams. However, approximately 70% of embryoless mangoes fail to meet this standard. This translates to approximately 1.5 million tons of mangoes annually failing to meet commercial standards and cannot be sold at commercial prices. This results in an annual loss of approximately 9.5 billion yuan due to embryoless mangoes. If all mangoes were embryonic, not only would this annual loss be reduced by approximately 9.5 billion yuan, but annual production could also be increased to 5.721 million tons. Without increasing existing land use, this would increase production by 1.14 times, and the output value by 28.8 billion yuan, a 1.69-fold increase. Therefore, it is necessary to develop a mango embryo-inducing agent and method to reduce the proportion of embryoless mangoes. Summary of the Invention

[0004] In response to the above problems, the present invention provides a mango embryo-promoting agent and an embryo-promoting method, which can significantly reduce the incidence of mango embryoless fruits, increase the mango yield of a single tree, and increase the income of fruit farmers.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] A mango embryo-promoting agent, comprising an inflorescence development stage embryo-promoting solution I;

[0007] The formula of the embryo-promoting solution I during the inflorescence development period comprises: MS culture solution, aminoethoxyvinylglycine (AVG), gibberellin A3 (GA3), imidacloprid, spinetoram, 6-benzylaminopurine (6-BA) and KH2PO4.

[0008] Furthermore, the inflorescence development period embryo-promoting solution I uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10~130ppm, the concentration of gibberellin A3 is 20~300ppm, the concentration of imidacloprid is 1~10g / L, the concentration of ethyl spinetoram is 10~100g / L, the concentration of 6-benzylaminopurine is 10~200mg / L and the concentration of KH2PO4 is 200~2000mg / L.

[0009] Furthermore, the mango embryo-promoting agent further comprises a flowering period embryo-promoting solution II;

[0010] The formula of the embryo promotion solution II at the peak flowering stage includes: MS culture solution, aminoethoxyvinylglycine (AVG), imidacloprid, spinetoram, gibberellin and complex amino acid trace element chelate.

[0011] Furthermore, the gibberellin used in the embryo promotion solution II at the peak flowering stage is gibberellin A3, gibberellin A4 or gibberellin A7;

[0012] The flowering stage embryo-promoting solution II uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of ethyl spinetoram is 10-100 g / L, the concentration of gibberellin is 10-50 mg / L, and the concentration of complex amino acid trace element chelate is 500-2500 mg / L.

[0013] Furthermore, the mango embryo-promoting agent further comprises a fruit expansion period embryo-promoting solution III;

[0014] The formula of the embryo-promoting solution III during the fruit expansion period includes: MS culture solution, aminoethoxyvinylglycine (AVG), gibberellin A3 (GA3), imidacloprid, spinetoram, indoleacetic acid (IAA) and urea.

[0015] Furthermore, the fruit expansion period embryo-promoting solution III uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10~130ppm, the concentration of gibberellin A3 is 20~300ppm, the concentration of imidacloprid is 1~10g / L, the concentration of ethyl spinetoram is 10~100g / L, the concentration of indoleacetic acid is 20~100mg / L and the concentration of urea is 1000~5000mg / L.

[0016] Furthermore, the mango embryo-promoting agent further comprises embryo-promoting solution IV after the expansion period;

[0017] The formula of the embryo promotion solution IV after the expansion period includes: MS culture medium, aminoethoxyvinylglycine (AVG), gibberellin A3 (GA3), imidacloprid, spinetoram, indoleacetic acid (IAA) and complex amino acid trace element chelate.

[0018] Furthermore, the embryo promotion solution IV after the expansion period uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10~130ppm, the concentration of gibberellin A is 20~300ppm, the concentration of imidacloprid is 1~10g / L, the concentration of ethyl spinetoram is 10~100g / L, the concentration of indoleacetic acid is 20~100mg / L and the concentration of complex amino acid trace element chelate is 500~3000mg / L.

[0019] A mango embryo promotion method is disclosed, which comprises applying the mango embryo promotion agent during the mango planting process to promote mango embryo development.

[0020] Furthermore, the mango embryo-promoting agent includes an embryo-promoting solution I for the inflorescence development stage, an embryo-promoting solution II for the flowering stage, an embryo-promoting solution III for the fruit expansion stage, and an embryo-promoting solution IV after the expansion stage;

[0021] During the mango planting process, applying the inflorescence development stage embryo promotion solution I at the early stage of inflorescence development;

[0022] Apply the flowering embryo promotion solution II during the flowering period;

[0023] Apply the fruit expansion embryo promotion solution III during the fruit expansion period;

[0024] Administer post-expansion embryo promotion solution IV 15 days after the expansion period;

[0025] The inflorescence development stage embryo promotion solution I uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of gibberellin A3 is 20-300 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of ethyl spinetoram is 10-100 g / L, the concentration of 6-benzylaminopurine is 10-200 mg / L, and the concentration of KH2PO4 is 200-2000 mg / L;

[0026] The blooming embryo promotion solution II uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of spinetoram is 10-100 g / L, the concentration of gibberellin is 10-50 mg / L, and the concentration of complex amino acid trace element chelate is 500-2500 mg / L; the gibberellin used in the blooming embryo promotion solution II is gibberellin A3, gibberellin A4 or gibberellin A7;

[0027] The fruit expansion stage embryo promotion solution III uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of gibberellin A3 is 20-300 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of ethyl spinetoram is 10-100 g / L, the concentration of indoleacetic acid is 20-100 mg / L, and the concentration of urea is 1000-5000 mg / L;

[0028] The post-expansion embryo promotion solution IV uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10~130ppm, the concentration of gibberellin A is 20~300ppm, the concentration of imidacloprid is 1~10g / L, the concentration of ethyl spinetoram is 10~100g / L, the concentration of indoleacetic acid is 20~100mg / L, and the concentration of complex amino acid trace element chelate is 500~3000mg / L.

[0029] The beneficial effects of the mango embryo-promoting agent and embryo-promoting method of the present invention are:

[0030] Since mango flowers are divided into male flowers and bisexual flowers, the mango flowers fall off on a large scale for the first time 5 days after fertilization, and the second large-scale flower drop occurs about 25 days after fertilization. The present invention uses AVG and GA3 to cooperate with each other to protect the mango flowers and reduce the proportion of mango flowers falling. Among them, because ethylene can promote the formation of the abscission layer of the pedicel and fruit stalk, promoting flower and fruit drop, and AVG is an ethylene inhibitor, it can block the synthesis of the ethylene precursor ACC, reduce the ethylene concentration, and reduce the proportion of mango flowers falling. At the same time, mango flower and fruit shedding occurs in the abscission layer, which has only two layers of densely arranged cells, and the xylem and phloem are broken. GA3 can promote the elongation of cells on both sides of the abscission layer, increase the connection between cells on both sides of the abscission layer, reduce the separation of the abscission layer, and reduce the proportion of mango flowers falling.

[0031] Embryo abortion during the development of young mango fruits after fertilization of mango flowers is the main cause of embryoless mango fruits. Embryo abortion is caused by insufficient nutrition supply and lack of auxin. The mango embryo-promoting agent of the present invention contains MS culture medium, complex amino acid trace element chelate and IAA, which can replenish the nutrients and auxin consumed during the development of young mango fruits and reduce the incidence of embryo abortion.

[0032] Mango pulp comes from the ovary wall, and the pericarp comes from the ovary epidermis. Inside the pulp is the seed, which consists of an endosperm and an embryo. The seed results from double fertilization, where the sperm combines with two polar nuclei to form a triploid endosperm, and the sperm combines with the egg to form an embryo. Mango fruit without embryos is essentially a result of the abortion of mango seed development. This abortion of mango seed development can be caused by various factors, including fertilization failure, insufficient nutrient supply after fertilization, and insufficient auxin content. The mango embryo-promoting agent of the present invention can increase the nutrition of mango fruits and reduce the proportion of embryoless fruits by supplementing MS culture medium and complex amino acid trace element chelates. Supplementing IAA can also replenish the auxin content of young mango fruits, promote embryo development, and reduce the proportion of embryoless fruits.

[0033] During the mango flowering and young fruit development period, thrips are more common, and most flowers and young fruits bitten by thrips fall off. The mango embryo-promoting agent formula of the present invention contains imidacloprid and ethyl spinetoram, which can effectively kill thrips and reduce the shedding of flowers and young fruits caused by thrips.

[0034] The mango embryo-promoting agent and method of the present invention can reduce the proportion of mango embryo-free fruits to about 80%, with a reduction range of about 10%. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a diagram of the hanging of the mango "Tainong No. 1" in Example 1 of the present invention;

[0036] Figure 2 This is a diagram of the hanging of the mango "Tainong No. 1" in Example 1 of the present invention;

[0037] Figure 3 This is a comparison diagram of the "Guifei" mango with and without embryos in Example 2 of the present invention;

[0038] Figure 4 This is a statistical diagram of embryonic and non-embryonic fruits of the "Guifei" mango in Example 2 of the present invention;

[0039] Figure 5 This is the embryo development test result of the "Guifei" mango in Example 2 of the present invention;

[0040] Figure 6 This is the embryo development test result of the "Guifei" mango in Example 2 of the present invention;

[0041] Figure 7 This is the "Golden Brilliant" mango hanging diagram in Example 3 of the present invention;

[0042] Figure 8 This is a comparison diagram of the "Jinhuang" mango with and without embryos in Example 3 of the present invention;

[0043] Figure 9This is a statistical diagram of embryonic fruits and embryoless fruits of the "Jinhuang" mango in Example 3 of the present invention. DETAILED DESCRIPTION

[0044] The technical solutions in the embodiments of the present invention are described clearly and completely below. Many specific details are set forth in the following description to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0045] Example 1 A method for promoting embryogenesis of mango

[0046] This embodiment is a method for promoting embryo growth in mangoes, and the specific process includes the following steps:

[0047] 1. Preparation of mango embryo promoter

[0048] In this embodiment, the mango embryo-promoting agent includes an embryo-promoting solution I during the inflorescence development period, an embryo-promoting solution II during the flowering period, an embryo-promoting solution III during the fruit expansion period, and an embryo-promoting solution IV after the expansion period. Specifically:

[0049] Aminoethoxyvinylglycine (AVG), gibberellin A3 (GA3), imidacloprid, spinetoram, 6-benzylaminopurine (6-BA), and KH2PO4 were added to MS culture medium and dissolved to prepare three different concentrations of inflorescence development stage embryo-promoting solutions I. The AVG concentrations in these solutions were 10, 62.5, and 125 ppm, respectively; the GA3 concentrations were 45, 135, and 270 ppm, respectively; the imidacloprid concentrations were 1, 2, and 3 g / L, respectively; the spinetoram concentrations were 30, 60, and 90 g / L, respectively; the 6-BA concentrations were 60, 90, and 120 mg / L, respectively; and the KH2PO4 concentrations were 1000, 1500, and 2000 mg / L, respectively.

[0050] Aminoethoxyvinylglycine (AVG), imidacloprid, spinetoram, gibberellin (GA3), gibberellin (A3, A4, or A7 can be used; in this example, gibberellin A3, i.e., GA3), and a complex amino acid trace element chelate (purchased from Shanghai Yuanye Biotechnology Co., Ltd.) were added to MS culture medium and dissolved to prepare three different concentrations of low, medium, and high concentrations of full-blooming embryo-promoting solutions II. The AVG concentrations in the three different concentrations of full-blooming embryo-promoting solutions II were 10, 62.5, and 125 ppm, respectively; the imidacloprid concentrations were 1, 2, and 3 g / L, respectively; the spinetoram concentrations were 30, 60, and 90 g / L, respectively; the GA3 concentrations were 20, 30, and 40 mg / L, respectively; and the complex amino acid trace element chelate concentrations were 1200, 1800, and 2400 mg / L, respectively.

[0051] Aminoethoxyvinylglycine (AVG), gibberellin A3 (GA3), imidacloprid, spinetoram, indoleacetic acid (IAA), and urea were added to MS culture medium and dissolved to prepare low, medium, and high concentrations of embryo-promoting solutions III for the fruit expansion period. The AVG concentrations in these solutions were 10, 62.5, and 125 ppm, respectively; the GA3 concentrations were 45, 135, and 270 ppm, respectively; the imidacloprid concentrations were 1, 2, and 3 g / L, respectively; the spinetoram concentrations were 30, 60, and 90 g / L, respectively; the IAA concentrations were 40, 60, and 80 mg / L, respectively; and the urea concentrations were 3000, 3500, and 4000 mg / L, respectively.

[0052] Aminoethoxyvinylglycine (AVG), gibberellin A3 (GA3), imidacloprid, spinetoram, indoleacetic acid (IAA), and complex amino acid trace element chelates (purchased from Shanghai Yuanye Biotechnology Co., Ltd.) were added to MS culture medium and dissolved to prepare three different concentrations of post-expansion embryo promotion solutions IV. The AVG concentrations in these solutions were 10, 62.5, and 125 ppm, respectively; the GA3 concentrations were 45, 135, and 270 ppm, respectively; the imidacloprid concentrations were 1, 2, and 3 g / L, respectively; the spinetoram concentrations were 30, 60, and 90 g / L, respectively; the IAA concentrations were 40, 60, and 80 mg / L, respectively; and the complex amino acid trace element chelates were 1200, 1800, and 2400 mg / L, respectively.

[0053] 2. Embryo promotion method

[0054] like Figure 1 and Figure 2 As shown in the figure, during the cultivation of "Tainong No. 1" mangoes, at the early stage of inflorescence development, 500 mL of inflorescence development embryo promotion solution I was applied to each tree. The dripping rate was adjusted and the dripping was completed in two days. After the dripping was completed, the second bag of inflorescence development embryo promotion solution I was applied at the same dripping rate until the flowering stage was reached. The inflorescence development embryo promotion solution I applied during the hanging application can be any of the three concentrations of inflorescence development embryo promotion solution I: low, medium, or high.

[0055] When the fruit enters the peak flowering stage, stop hanging application of Inflorescence Development Stage Embryo Promotion Solution I. Instead, hang application of 500 mL of Peak Flowering Stage Embryo Promotion Solution II to each tree at an adjusted drip rate. After dripping, continue hanging application of a second bag of Peak Flowering Stage Embryo Promotion Solution II at the same drip rate until the fruit enters the expansion stage. The Peak Flowering Stage Embryo Promotion Solution II applied can be any of the three concentrations of Peak Flowering Stage Embryo Promotion Solution II: low, medium, or high.

[0056] During the fruit expansion phase, stop hanging application of the flowering stage embryo promotion solution II. Instead, hang application of 500 mL of the fruit expansion stage embryo promotion solution III to each tree at an adjusted drip rate over two days. After the dripping is complete, continue hanging application of a second bag of the fruit expansion stage embryo promotion solution III at the same drip rate until the fruit expansion phase ends. The fruit expansion stage embryo promotion solution III can be applied at any of the three concentrations of low, medium, or high.

[0057] After the fruit expansion period ends, stop hanging application of Fruit Expansion Period Embryo-Promoting Solution III. Continue growing for 14 days, i.e., 15 days after the expansion period. Hang apply 500 mL of Post-Expansion Period Embryo-Promoting Solution IV to each tree. Adjust the drip rate and drip the solution over two days. After dripping is complete, continue hanging application of a second bag of Post-Expansion Period Embryo-Promoting Solution IV at the same drip rate until the mangoes are ripe. The Post-Expansion Period Embryo-Promoting Solution IV applied can be any of the three concentrations of Post-Expansion Period Embryo-Promoting Solution IV: low, medium, or high.

[0058] Mangoes were harvested after they were ripe, the fruits were cut open, and the incidence of embryoless fruits was counted.

[0059] In the specific implementation of the above-mentioned embryo promotion method in this example, a mango group that was not subjected to hanging application of embryo promotion solution I during inflorescence development, embryo promotion solution II during full flowering, embryo promotion solution III during fruit expansion, and embryo promotion solution IV after expansion was used as a blank control group;

[0060] The mango group that was only fertilized with high concentration of embryo promotion solution I during inflorescence development was the experimental group.

[0061] The mango group that was only fertilized with high concentration of embryo promotion solution II at the blooming stage was the second experimental group;

[0062] The mango group that was only fertilized with high concentration of embryo-promoting solution III during the fruit expansion period was the third experimental group;

[0063] The mango group that was only fertilized with high concentration of embryo promotion solution IV after the expansion period was the fourth experimental group;

[0064] The mango group that received high-concentration embryo-promoting solution I during inflorescence development and high-concentration embryo-promoting solution II during full flowering was the fifth experimental group (i.e., no embryo-promoting solution III during fruit expansion and embryo-promoting solution IV after expansion was applied).

[0065] The mango group that was fertilized with high concentrations of embryo-promoting solution I during the inflorescence development period and high concentrations of embryo-promoting solution III during the fruit expansion period was the sixth experimental group (i.e., no embryo-promoting solution II during the flowering period and embryo-promoting solution IV after the expansion period were fertilized).

[0066] The mango group that was fertilized with high concentrations of embryo-promoting solution I during the inflorescence development stage and embryo-promoting solution IV after the expansion stage was the seventh experimental group (i.e., no embryo-promoting solution II during the flowering stage and embryo-promoting solution III during the fruit expansion stage were fertilized with high concentrations of solution I during the inflorescence development stage and embryo-promoting solution IV after the fruit expansion stage);

[0067] The mango groups that were fertilized with high concentrations of embryo-promoting solution I during inflorescence development, high concentrations of embryo-promoting solution II during flowering, and high concentrations of embryo-promoting solution III during fruit expansion were the eighth experimental group (i.e., embryo-promoting solution IV after the expansion period was not fertilized).

[0068] The mango group that was fertilized with high concentrations of embryo-promoting solution I during the inflorescence development period, high concentrations of embryo-promoting solution II during the flowering period, and high concentrations of embryo-promoting solution IV after the expansion period was the ninth experimental group (i.e., embryo-promoting solution III during the fruit expansion period was not fertilized).

[0069] The mango group that was fertilized with high concentrations of embryo-promoting solution I during the inflorescence development period, high concentrations of embryo-promoting solution III during the fruit expansion period, and high concentrations of embryo-promoting solution IV after the expansion period was the tenth experimental group (i.e., no embryo-promoting solution II during the flowering period was fertilized).

[0070] The mango group that was fertilized with high concentrations of embryo-promoting solution II during the flowering stage, high concentrations of embryo-promoting solution III during the fruit expansion stage, and high concentrations of embryo-promoting solution IV after the expansion stage was the eleventh experimental group (i.e., embryo-promoting solution I during the inflorescence development stage was not fertilized);

[0071] The mango groups that were fertilized with high concentrations of embryo-promoting solution I during inflorescence development, high concentrations of embryo-promoting solution II during flowering, high concentrations of embryo-promoting solution III during fruit expansion, and high concentrations of embryo-promoting solution IV after the expansion stage were the twelve experimental groups.

[0072] The results showed that the incidence of mango without embryo fruit in the blank control group was 90%, the incidence of mango without embryo fruit in the experimental group was 85%, the incidence of mango without embryo fruit in the experimental group 2 was 87%, the incidence of mango without embryo fruit in the experimental group 3 was 86%, the incidence of mango without embryo fruit in the experimental group 4 was 88%, the incidence of mango without embryo fruit in the experimental group 5 was 80%, the incidence of mango without embryo fruit in the experimental group 6 was 83%, the incidence of mango without embryo fruit in the experimental group 7 was 78%, the incidence of mango without embryo fruit in the experimental group 8 was 73%, the incidence of mango without embryo fruit in the experimental group 9 was 75%, the incidence of mango without embryo fruit in the experimental group 10 was 77%, the incidence of mango without embryo fruit in the experimental group 11 was 74%, and the incidence of mango without embryo fruit in the experimental group 12 was 73%. It can be seen from this that the use of the mango embryo-promoting agent of the present invention can reduce the incidence of mango without embryo fruit, especially when the mango embryo-promoting agent of the present invention is used in combination, the incidence of mango without embryo fruit can be significantly reduced.

[0073] Example 2 A method for promoting embryogenesis of mango

[0074] This embodiment is a method for promoting embryo growth in mangoes, and the specific process includes the following steps:

[0075] 1. Preparation of mango embryo promoter

[0076] The mango embryo-promoting agent in this embodiment includes an embryo-promoting solution I for the inflorescence development stage, an embryo-promoting solution II for the full flowering stage, an embryo-promoting solution III for the fruit expansion stage, and an embryo-promoting solution IV after the expansion stage. The formula and preparation process are basically the same as those in Example 1, with the only difference being that:

[0077] In the preparation of low, medium, and high concentrations of the peak-flowering embryo-stimulating solution II, gibberellin A4 (GA4) was used instead of gibberellin A3 (GA3). The concentrations of GA4 in the low, medium, and high concentrations of the peak-flowering embryo-stimulating solution II were 20, 30, and 40 mg / L, respectively. The other raw materials, amounts, and preparation procedures for the low, medium, and high concentrations of the peak-flowering embryo-stimulating solution II were identical to those in Example 1.

[0078] 2. Embryo promotion method

[0079] During the cultivation of mango "Guifei", the above-mentioned mango embryo-promoting agent was applied by hanging according to the embryo-promoting method in Example 1, and the mango group that was not applied with embryo-promoting solution I during inflorescence development, embryo-promoting solution II during blooming, embryo-promoting solution III during fruit expansion, and embryo-promoting solution IV after expansion was used as a blank control group;

[0080] The mango group that was only fertilized with low concentration of embryo promotion solution I during inflorescence development was the experimental group;

[0081] The mango group that was only fertilized with low concentration of embryo promotion solution II at the blooming stage was the second experimental group;

[0082] The mango group that was only fertilized with low concentration of embryo promotion solution III during the fruit expansion period was the third experimental group;

[0083] The mango group that was only fertilized with low concentration of embryo promotion solution IV after the expansion period was the fourth experimental group;

[0084] The mango group that was fertilized with low-concentration embryo-promoting solution I during the inflorescence development stage and low-concentration embryo-promoting solution II during the flowering stage was the fifth experimental group (i.e., no embryo-promoting solution III during the fruit expansion stage and embryo-promoting solution IV after the expansion stage was fertilized).

[0085] The mango group that was fertilized with low-concentration embryo-promoting solution I during the inflorescence development period and low-concentration embryo-promoting solution III during the fruit expansion period was the sixth experimental group (i.e., no embryo-promoting solution II during the flowering period and embryo-promoting solution IV after the expansion period were fertilized).

[0086] The mango group that was fertilized with low concentrations of embryo-promoting solution I during the inflorescence development stage and embryo-promoting solution IV after the expansion stage was the seventh experimental group (i.e., no embryo-promoting solution II during the flowering stage and embryo-promoting solution III during the fruit expansion stage were fertilized with low concentrations of solution I during the inflorescence development stage and embryo-promoting solution IV after the fruit expansion stage);

[0087] The mango groups that were fertilized with low-concentration embryo-stimulating solution I during inflorescence development, low-concentration embryo-stimulating solution II during flowering, and low-concentration embryo-stimulating solution III during fruit expansion were the eighth experimental group (i.e., embryo-stimulating solution IV after expansion was not fertilized).

[0088] The mango group that was fertilized with low concentrations of embryo-promoting solution I during the inflorescence development period, low concentrations of embryo-promoting solution II during the flowering period, and low concentrations of embryo-promoting solution IV after the expansion period was the ninth experimental group (i.e., embryo-promoting solution III during the fruit expansion period was not fertilized).

[0089] The mango group that was fertilized with low concentrations of embryo-promoting solution I during the inflorescence development period, low concentrations of embryo-promoting solution III during the fruit expansion period, and low concentrations of embryo-promoting solution IV after the expansion period was the tenth experimental group (i.e., embryo-promoting solution II during the flowering period was not fertilized).

[0090] The mango group that was fertilized with low concentrations of embryo-promoting solution II during the flowering stage, low concentrations of embryo-promoting solution III during the fruit expansion stage, and low concentrations of embryo-promoting solution IV after the expansion stage was the eleventh experimental group (i.e., embryo-promoting solution I during the inflorescence development stage was not fertilized).

[0091] The twelve mango groups were treated with low-concentration embryo-promoting solution I during inflorescence development, low-concentration embryo-promoting solution II during flowering, low-concentration embryo-promoting solution III during fruit expansion, and low-concentration embryo-promoting solution IV after expansion. Figure 3 As shown in the figure, the statistical graph of embryonic fruits and embryoless fruits is as follows Figure 4 As shown; Mango embryo development examination as shown Figure 5 and Figure 6 shown.

[0092] The results showed that the incidence of mango without embryo fruit in the blank control group was 92%, the incidence of mango without embryo fruit in the experimental group was 89%, the incidence of mango without embryo fruit in the experimental group 2 was 90%, the incidence of mango without embryo fruit in the experimental group 3 was 88%, the incidence of mango without embryo fruit in the experimental group 4 was 87%, the incidence of mango without embryo fruit in the experimental group 5 was 85%, the incidence of mango without embryo fruit in the experimental group 6 was 83%, the incidence of mango without embryo fruit in the experimental group 7 was 86%, the incidence of mango without embryo fruit in the experimental group 8 was 84%, the incidence of mango without embryo fruit in the experimental group 9 was 83%, the incidence of mango without embryo fruit in the experimental group 10 was 82%, the incidence of mango without embryo fruit in the experimental group 11 was 84%, and the incidence of mango without embryo fruit in the experimental group 12 was 81%. It can be seen that the use of the mango embryo-promoting agent of the present invention can reduce the incidence of mango without embryo fruit, especially when the mango embryo-promoting agent of the present invention is used in combination, the incidence of mango without embryo fruit can be significantly reduced.

[0093] Example 3 A method for promoting embryogenesis of mango

[0094] This embodiment is a method for promoting embryo growth in mangoes, and the specific process includes the following steps:

[0095] 1. Preparation of mango embryo promoter

[0096] The mango embryo-promoting agent in this embodiment includes an embryo-promoting solution I for the inflorescence development stage, an embryo-promoting solution II for the full flowering stage, an embryo-promoting solution III for the fruit expansion stage, and an embryo-promoting solution IV after the expansion stage. The formula and preparation process are basically the same as those in Example 1, with the only difference being that:

[0097] In the preparation of low, medium, and high concentrations of the flowering-stage embryo-stimulating solution II, gibberellin A7 (GA7) was used instead of gibberellin A7 (GA7). The concentrations of GA7 in the low, medium, and high concentrations of the flowering-stage embryo-stimulating solution II were 20, 30, and 40 mg / L, respectively. The other raw materials, amounts, and preparation procedures for the low, medium, and high concentrations of the flowering-stage embryo-stimulating solution II were identical to those in Example 1.

[0098] 2. Embryo promotion method

[0099] like Figure 7 As shown, during the cultivation of mango "Jinhuang", the above-mentioned mango embryo-promoting agent was applied by hanging according to the embryo-promoting method in Example 1, and the mango group to which embryo-promoting solution I in the inflorescence development stage, embryo-promoting solution II in the full flowering stage, embryo-promoting solution III in the fruit expansion stage, and embryo-promoting solution IV after the expansion stage were not applied by hanging was used as a blank control group;

[0100] The mango group that was only fertilized with medium concentration of embryo promotion solution I during inflorescence development was the experimental group.

[0101] The mango group that was only fertilized with medium concentration of embryo promotion solution II at the peak flowering stage was the second experimental group;

[0102] The mango group that was only fertilized with medium concentration of embryo-promoting solution III during the fruit expansion period was the third experimental group;

[0103] The mango group that was only fertilized with medium concentration of embryo promotion solution IV after the expansion period was the fourth experimental group;

[0104] The mango group that was fertilized with medium concentrations of embryo-promoting solution I during the inflorescence development stage and medium concentrations of embryo-promoting solution II during the flowering stage was the fifth experimental group (i.e., no embryo-promoting solution III during the fruit expansion stage and embryo-promoting solution IV after the expansion stage was fertilized).

[0105] The mango group that was fertilized with medium concentrations of embryo-promoting solution I during the inflorescence development stage and medium concentrations of embryo-promoting solution III during the fruit expansion stage was the sixth experimental group (i.e., no embryo-promoting solution II during the flowering stage and embryo-promoting solution IV after the expansion stage were fertilized).

[0106] The mango group that received medium concentrations of embryo-promoting solution I during the inflorescence development stage and embryo-promoting solution IV after the expansion stage was the seventh experimental group (i.e., no embryo-promoting solution II during the flowering stage and embryo-promoting solution III during the fruit expansion stage were applied).

[0107] The mango group that received medium concentrations of embryo promotion solution I during inflorescence development, medium concentrations of embryo promotion solution II during flowering, and medium concentrations of embryo promotion solution III during fruit expansion was the eighth experimental group (i.e., embryo promotion solution IV after expansion was not applied).

[0108] The mango group that received medium concentrations of embryo-promoting solution I during the inflorescence development period, medium concentrations of embryo-promoting solution II during the flowering period, and medium concentrations of embryo-promoting solution IV after the expansion period was the ninth experimental group (i.e., embryo-promoting solution III during the fruit expansion period was not applied).

[0109] The mango group that was fertilized with medium concentrations of embryo-promoting solution I during the inflorescence development period, medium concentrations of embryo-promoting solution III during the fruit expansion period, and medium concentrations of embryo-promoting solution IV after the expansion period was the tenth experimental group (i.e., no embryo-promoting solution II during the flowering period was fertilized).

[0110] The mango group that was fertilized with medium concentrations of embryo-stimulating solution II during the flowering stage, medium concentrations of embryo-stimulating solution III during the fruit expansion stage, and medium concentrations of embryo-stimulating solution IV after the expansion stage was the eleventh experimental group (i.e., embryo-stimulating solution I during the inflorescence development stage was not fertilized).

[0111] The mango groups that were applied with medium concentrations of embryo-promoting solution I during inflorescence development, medium concentrations of embryo-promoting solution II during flowering, medium concentrations of embryo-promoting solution III during fruit expansion, and medium concentrations of embryo-promoting solution IV after expansion were the twelve experimental groups.

[0112] The results showed that the incidence of mango without embryo fruit in the blank control group was 93%, the incidence of mango without embryo fruit in the experimental group was 90%, the incidence of mango without embryo fruit in the experimental group 2 was 89%, the incidence of mango without embryo fruit in the experimental group 3 was 91%, the incidence of mango without embryo fruit in the experimental group 4 was 90%, the incidence of mango without embryo fruit in the experimental group 5 was 88%, the incidence of mango without embryo fruit in the experimental group 6 was 89%, the incidence of mango without embryo fruit in the experimental group 7 was 87%, the incidence of mango without embryo fruit in the experimental group 8 was 86%, the incidence of mango without embryo fruit in the experimental group 9 was 85%, the incidence of mango without embryo fruit in the experimental group 10 was 83%, the incidence of mango without embryo fruit in the experimental group 11 was 82%, and the incidence of mango without embryo fruit in the experimental group 12 was 80%. It can be seen from this that the use of the mango embryo-promoting agent of the present invention can reduce the incidence of mango without embryo fruit, especially when the mango embryo-promoting agent of the present invention is used in combination, the incidence of mango without embryo fruit can be significantly reduced.

[0113] Example 4 A method for promoting embryogenesis of mango

[0114] This embodiment is a method for promoting embryo growth in mangoes, and the specific process includes the following steps:

[0115] 1. Preparation of mango embryo promoter

[0116] The mango embryo-promoting agent in this embodiment includes an embryo-promoting solution I for the inflorescence development stage, an embryo-promoting solution II for the flowering stage, an embryo-promoting solution III for the fruit expansion stage, and an embryo-promoting solution IV after the expansion stage. The formula and preparation process are exactly the same as those in Example 1.

[0117] 2. Embryo promotion method

[0118] During the cultivation of mango "Kate", the above-mentioned mango embryo-promoting agent was applied by hanging according to the embryo-promoting method in Example 1, and a mango group that was not applied with embryo-promoting solution I during inflorescence development, embryo-promoting solution II during full flowering, embryo-promoting solution III during fruit expansion, and embryo-promoting solution IV after the expansion period was used as a blank control group;

[0119] The mango group that was only fertilized with medium concentration of embryo promotion solution I during inflorescence development was the experimental group.

[0120] The mango group that was only fertilized with medium concentration of embryo promotion solution II at the peak flowering stage was the second experimental group;

[0121] The mango group that was only fertilized with medium concentration of embryo-promoting solution III during the fruit expansion period was the third experimental group;

[0122] The mango group that was only fertilized with medium concentration of embryo promotion solution IV after the expansion period was the fourth experimental group;

[0123] The mango group that was fertilized with medium concentrations of embryo-promoting solution I during the inflorescence development stage and medium concentrations of embryo-promoting solution II during the flowering stage was the fifth experimental group (i.e., no embryo-promoting solution III during the fruit expansion stage and embryo-promoting solution IV after the expansion stage was fertilized).

[0124] The mango group that was fertilized with medium concentrations of embryo-promoting solution I during the inflorescence development stage and medium concentrations of embryo-promoting solution III during the fruit expansion stage was the sixth experimental group (i.e., no embryo-promoting solution II during the flowering stage and embryo-promoting solution IV after the expansion stage were fertilized).

[0125] The mango group that was fertilized with medium concentrations of embryo-stimulating solution I during the inflorescence development stage and embryo-stimulating solution IV after the expansion stage was the seventh experimental group (i.e., no embryo-stimulating solution II during the flowering stage and embryo-stimulating solution III during the fruit expansion stage were fertilized with medium concentrations of solution I during the inflorescence development stage and embryo-stimulating solution IV after the fruit expansion stage);

[0126] The mango group that received medium concentrations of embryo promotion solution I during inflorescence development, medium concentrations of embryo promotion solution II during flowering, and medium concentrations of embryo promotion solution III during fruit expansion was the eighth experimental group (i.e., embryo promotion solution IV after expansion was not applied).

[0127] The mango group that received medium concentrations of embryo-promoting solution I during the inflorescence development period, medium concentrations of embryo-promoting solution II during the flowering period, and medium concentrations of embryo-promoting solution IV after the expansion period was the ninth experimental group (i.e., embryo-promoting solution III during the fruit expansion period was not applied).

[0128] The mango group that was fertilized with medium concentrations of embryo-promoting solution I during the inflorescence development period, medium concentrations of embryo-promoting solution III during the fruit expansion period, and medium concentrations of embryo-promoting solution IV after the expansion period was the tenth experimental group (i.e., no embryo-promoting solution II during the flowering period was fertilized).

[0129] The mango group that was fertilized with medium concentrations of embryo-stimulating solution II during the flowering stage, medium concentrations of embryo-stimulating solution III during the fruit expansion stage, and medium concentrations of embryo-stimulating solution IV after the expansion stage was the eleventh experimental group (i.e., embryo-stimulating solution I during the inflorescence development stage was not fertilized).

[0130] The mango groups that received medium concentrations of embryo-promoting solution I during the inflorescence development period, medium concentrations of embryo-promoting solution II during the flowering period, medium concentrations of embryo-promoting solution III during the fruit expansion period, and medium concentrations of embryo-promoting solution IV after the expansion period were the twelve experimental groups. Figure 8 As shown in the figure, the statistical graph of embryonic fruits and embryoless fruits is as follows Figure 9 shown.

[0131] The results showed that the incidence of mango without embryo fruit in the blank control group was 94%, the incidence of mango without embryo fruit in the experimental group was 90%, the incidence of mango without embryo fruit in the experimental group 2 was 91%, the incidence of mango without embryo fruit in the experimental group 3 was 89%, the incidence of mango without embryo fruit in the experimental group 4 was 90%, the incidence of mango without embryo fruit in the experimental group 5 was 87%, the incidence of mango without embryo fruit in the experimental group 6 was 85%, the incidence of mango without embryo fruit in the experimental group 7 was 86%, the incidence of mango without embryo fruit in the experimental group 8 was 85%, the incidence of mango without embryo fruit in the experimental group 9 was 84%, the incidence of mango without embryo fruit in the experimental group 10 was 82%, the incidence of mango without embryo fruit in the experimental group 11 was 84%, and the incidence of mango without embryo fruit in the experimental group 12 was 78%. It can be seen that the use of the mango embryo-promoting agent of the present invention can reduce the incidence of mango without embryo fruit, especially when the mango embryo-promoting agent of the present invention is used in combination, the incidence of mango without embryo fruit can be significantly reduced.

[0132] Examples 5-6 Mango embryo promoter

[0133] Examples 5 and 6 are respectively a mango embryo-promoting agent, and their preparation steps are basically the same as those of Example 1, except that the ratio of raw materials is different. See Table 1 for details:

[0134] Table 1 List of process parameters in Examples 5 and 6

[0135]

[0136] The other contents of Examples 5 and 6 are the same as those of Example 1.

[0137] Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

Claims

1. A mango embryo-promoting agent, characterized in that The mango embryo-promoting agent comprises an embryo-promoting solution I during the inflorescence development period; The inflorescence development stage embryo-promoting solution I uses MS culture medium as a solvent, which contains aminoethoxyvinylglycine at a concentration of 10~130ppm, gibberellin A3 at a concentration of 20~300ppm, imidacloprid at a concentration of 1~10g / L, ethyl spinetoram at a concentration of 10~100g / L, 6-benzylaminopurine at a concentration of 10~200mg / L and KH2PO4 at a concentration of 200~2000mg / L.

2. The mango embryo-promoting agent according to claim 1, characterized in that The mango embryo-promoting agent also includes a flowering period embryo-promoting solution II; The flowering stage embryo-promoting solution II uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of ethyl spinetoram is 10-100 g / L, the concentration of gibberellin is 10-50 mg / L, and the concentration of complex amino acid trace element chelate is 500-2500 mg / L.

3. The mango embryo-promoting agent according to claim 2, characterized in that The gibberellin used in the embryo-promoting solution II during the flowering period is gibberellin A3, gibberellin A4 or gibberellin A7.

4. The mango embryo-promoting agent according to any one of claims 1 to 3, characterized in that The mango embryo-promoting agent also includes a fruit expansion period embryo-promoting solution III; The fruit expansion period embryo-promoting solution III uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of gibberellin A3 is 20-300 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of ethyl spinetoram is 10-100 g / L, the concentration of indoleacetic acid is 20-100 mg / L, and the concentration of urea is 1000-5000 mg / L.

5. The mango embryo-promoting agent according to claim 4, characterized in that The mango embryo-promoting agent further comprises an embryo-promoting solution IV after the expansion period; The post-expansion embryo promotion solution IV uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10~130ppm, the concentration of gibberellin A3 is 20~300ppm, the concentration of imidacloprid is 1~10g / L, the concentration of ethyl spinetoram is 10~100g / L, the concentration of indoleacetic acid is 20~100mg / L, and the concentration of complex amino acid trace element chelate is 500~3000mg / L.

6. The mango embryo-promoting agent according to any one of claims 1 to 3, characterized in that The mango embryo-promoting agent further comprises an embryo-promoting solution IV after the expansion period; The post-expansion embryo promotion solution IV uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10~130ppm, the concentration of gibberellin A3 is 20~300ppm, the concentration of imidacloprid is 1~10g / L, the concentration of ethyl spinetoram is 10~100g / L, the concentration of indoleacetic acid is 20~100mg / L, and the concentration of complex amino acid trace element chelate is 500~3000mg / L.

7. A method for promoting embryogenesis of mango, characterized in that: The mango embryo promotion method comprises applying the mango embryo promotion agent according to any one of claims 1 to 6 during the mango planting process to promote the embryo development of mango.

8. The method for promoting embryogenesis of mango according to claim 7, characterized in that: The mango embryo-promoting agent includes an embryo-promoting solution I during the inflorescence development period, an embryo-promoting solution II during the flowering period, an embryo-promoting solution III during the fruit expansion period, and an embryo-promoting solution IV after the expansion period. During the mango planting process, applying the inflorescence development stage embryo promotion solution I at the early stage of inflorescence development; Apply the flowering embryo promotion solution II during the flowering period; Apply the fruit expansion embryo promotion solution III during the fruit expansion period; Administer post-expansion embryo promotion solution IV 15 days after the expansion period; The inflorescence development stage embryo promotion solution I uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of gibberellin A3 is 20-300 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of ethyl spinetoram is 10-100 g / L, the concentration of 6-benzylaminopurine is 10-200 mg / L, and the concentration of KH2PO4 is 200-2000 mg / L; The blooming embryo promotion solution II uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of spinetoram is 10-100 g / L, the concentration of gibberellin is 10-50 mg / L, and the concentration of complex amino acid trace element chelate is 500-2500 mg / L; the gibberellin used in the blooming embryo promotion solution II is gibberellin A3, gibberellin A4 or gibberellin A7; The fruit expansion stage embryo promotion solution III uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of gibberellin A3 is 20-300 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of ethyl spinetoram is 10-100 g / L, the concentration of indoleacetic acid is 20-100 mg / L, and the concentration of urea is 1000-5000 mg / L; The post-expansion embryo promotion solution IV uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10~130ppm, the concentration of gibberellin A3 is 20~300ppm, the concentration of imidacloprid is 1~10g / L, the concentration of ethyl spinetoram is 10~100g / L, the concentration of indoleacetic acid is 20~100mg / L, and the concentration of complex amino acid trace element chelate is 500~3000mg / L.

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