Traditional Chinese medicine feed additive for promoting growth and preventing bacterial wilt as well as preparation method and application of traditional Chinese medicine feed additive

Abstract

The invention provides a traditional Chinese medicine feed additive for promoting growth and preventing bacterial wilt as well as a preparation method and application of the traditional Chinese medicine feed additive, and belongs to the technical field of traditional Chinese medicine feed additives, and the IPC classification number is A23K10. The traditional Chinese medicine feed additive comprises a traditional Chinese medicine extract and a complex microbial inoculant, the traditional Chinese medicine extract is extracted from radix gentianae, fructus galangae, folium isatidis, carpesium abrotanoides, betel nuts, cinnamon leaves, betel pepper, coix seed roots, rhizoma cimicifugae, liquorice and purple perilla; the complex microbial inoculants comprise digestion associated lactobacillus and rhamnosus cheese bacillus. The traditional Chinese medicine extract is subjected to a three-in-one mechanism of digestion promotion, immune activation and pathogen inhibition, and the complex microbial inoculant can improve the feed utilization rate, promote absorption of the traditional Chinese medicine extract and enhance systemic immune response. Combined use of the traditional Chinese medicine extract and the complex microbial inoculant can realize synergistic interaction of healthy growth of animals and disease prevention and control, and is especially suitable for an ecological breeding mode for replacing antibiotics.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine feed additives. The IPC classification number belongs to A23K10. Specifically, it relates to a traditional Chinese medicine feed additive for promoting growth and preventing epidemic diseases, its preparation method and application. Background Art

[0002] With the rapid development of the livestock industry, feed additives play an important role in improving animal production performance, enhancing disease resistance and improving the quality of livestock products. However, the long-term use of traditional additives such as antibiotics, chemically synthesized drugs and steroid hormones has gradually exposed problems such as drug resistance, residual pollution and ecological risks. For example, the abuse of antibiotics has led to an increase in the drug resistance of pathogenic bacteria, seriously threatening public health safety; the residues of chemically synthesized drugs directly affect the safety of livestock products, causing consumers' concerns about food safety. In this context, the development of natural, safe and efficient alternatives has become an urgent need in the industry.

[0003] In response to the above problems, traditional Chinese medicine feed additives have gradually become a research hotspot due to their natural properties, multi-target effects and low residue characteristics. Although traditional Chinese medicine feed additives have significant advantages, there are still limitations in existing research: most formulations focus on single functions (such as promoting growth or preventing diseases), lacking comprehensive prevention and control programs for multiple epidemic diseases; some preparation methods (such as simple crushing and mixing) fail to fully release the active ingredients, resulting in low bioavailability. Therefore, the development of a traditional Chinese medicine feed additive that can double improve growth performance and disease resistance is of great practical significance for promoting the sustainable development of the livestock industry. Summary of the Invention

[0004] The purpose of the present invention is to provide a traditional Chinese medicine feed additive for promoting growth and preventing epidemic diseases, its preparation method and application. The traditional Chinese medicine feed additive provided by the present invention can improve the body immunity of animals, enhance disease resistance and promote growth.

[0005] In order to achieve the above invention purpose, the present invention provides the following technical solutions: The present invention provides a traditional Chinese medicine feed additive for promoting growth and preventing epidemic diseases, comprising the following raw materials in parts by weight: 8-12 parts of traditional Chinese medicine extract and 1-5 parts of compound bacterial agent; the traditional Chinese medicine extract is obtained by extracting gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut, cinnamon leaf, piper betle, coix root, cimicifuga foetida, liquorice and perilla; the compound bacterial agent includes lactobacillus companion digestus and lactobacillus rhamnosus.

[0006] Preferably, the weight ratio of gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut, cinnamon leaf, piper betle, coix root, cimicifuga foetida, liquorice and perilla is (5-8):(3-5):(5-8):(2-3):(3-5):(4-6):(2-4):(6-10):(3-5):(10-15):(8-12).

[0007] Preferably, the viable count of Lactobacillus alimentarius in the compound bacterial agent is 300 million - 800 million CFU / g, and the viable count of Lactobacillus rhamnosus is 800 million - 1.2 billion CFU / g.

[0008] The present invention also provides a preparation method of the above-mentioned traditional Chinese medicine feed additive, including: pulverizing gentian, galangal, folium isatidis, carpesium abrotanoides, betel nut powder and cinnamon leaf, adding a eutectic solvent and water, performing ultrasonic extraction, centrifuging, purifying, concentrating and drying to obtain material 1; pulverizing betel pepper, coix root, cimicifuga foetida, liquorice and perilla, adding an ethanol solution for ultrasonic extraction, centrifuging, concentrating and drying to obtain material 2; mixing material 1 and material 2 to obtain a traditional Chinese medicine extract, and adding a compound bacterial agent to obtain the traditional Chinese medicine feed additive.

[0009] Preferably, the extraction solvent is a eutectic solvent and water, the eutectic solvent is composed of choline chloride and isopropanol according to a molar ratio of 1:1.5 - 2.5, and the weight ratio of the eutectic solvent to water is 1 - 3:2 - 6.

[0010] More preferably, the temperature of the ultrasonic extraction for obtaining material 1 is 45 - 55°C, the power is 200 - 300W, the number of times is 1 - 2 times, and each extraction is 10 - 20 minutes.

[0011] Preferably, in the ultrasonic extraction with the ethanol solution, the volume percentage content of the ethanol solution is 60% - 70%, the temperature is 50 - 60°C, the power is 200 - 300W, the number of times is 2 - 3 times, and each time is 20 - 30 minutes.

[0012] The present invention also provides the application of the above-mentioned traditional Chinese medicine feed additive in the preparation of a feed for promoting growth and preventing epidemic diseases.

[0013] Preferably, the traditional Chinese medicine feed additive is used for the feeds of fish, poultry and livestock.

[0014] More preferably, the dosage of the traditional Chinese medicine feed additive is 3‰ - 8‰ of the feed weight.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a traditional Chinese medicine feed additive for promoting growth and preventing plague, comprising: a traditional Chinese medicine extract and a compound bacterium agent; the traditional Chinese medicine extract is obtained by extracting gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut, cinnamon leaf, betel pepper, coix root, cimicifuga foetida, liquorice and perilla; the compound bacterium agent includes lactobacillus alimentarius and lactobacillus rhamnosus. The traditional Chinese medicine extract acts through a trinity mechanism of "digestion promotion - immune activation - pathogen inhibition", and the compound bacterium agent can improve the feed utilization rate, promote the absorption of the traditional Chinese medicine extract, and enhance the systemic immune response. The combination of the traditional Chinese medicine extract and the compound bacterium agent in the present invention can achieve synergistic effects in the healthy growth of animals and disease prevention and control, and is particularly suitable for the ecological breeding mode that replaces antibiotics. Detailed implementation mode

[0016] The present invention provides a traditional Chinese medicine feed additive for promoting growth and preventing plague, comprising the following raw materials in parts by weight: 8-12 parts of a traditional Chinese medicine extract and 1-5 parts of a compound bacterium agent; the traditional Chinese medicine extract is obtained by extracting gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut, cinnamon leaf, betel pepper, coix root, cimicifuga foetida, liquorice and perilla; the compound bacterium agent includes lactobacillus alimentarius and lactobacillus rhamnosus.

[0017] When used as a traditional Chinese medicine feed additive for pigs, the traditional Chinese medicine extract further includes iris.

[0018] The weight ratio of gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut, cinnamon leaf, betel pepper, coix root, cimicifuga foetida, liquorice and perilla in the present invention is preferably (5-8):(3-5):(5-8):(2-3):(3-5):(4-6):(2-4):(6-10):(3-5):(10-15):(8-12), more preferably 6:4:6:2.5:4:5:3:8:4:12:10. When used as a traditional Chinese medicine feed additive for pigs, the weight ratio of gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut, cinnamon leaf, betel pepper, coix root, cimicifuga foetida, liquorice, perilla and iris in the traditional Chinese medicine additive is (5-8):(3-5):(5-8):(2-3):(3-5):(4-6):(2-4):(6-10):(3-5):(10-15):(8-12):(5-8), more preferably 6:4:6:2.5:4:5:3:8:4:12:10:6.

[0019] The Chinese herbal medicine combination adopted in the present invention has the following effects: (1) Multi-target synergy and intestinal health: Amomum galanga regulates the flora, Areca nut expels worms, and Cinnamomum cassia leaf inhibits bacteria, forming an intestinal microecological balance; Metabolic optimization: Gentiana scabra stimulates the appetite, Piper betle promotes protein synthesis, and Coix root promotes diuresis, improving the energy conversion efficiency; Immune enhancement: Isatis indigotica antiviral, Cimicifuga foetida activates the immune system, and Glycyrrhiza uralensis anti-stress, constructing a three-dimensional defense system. (2) Reducing the dependence on antibiotics, through natural antibacterial (Cinnamomum cassia leaf, Carpesium abrotanoides) and antiviral (Isatis indigotica) components, reducing the risk of bacterial drug resistance, and being applicable to the antibiotic-free breeding scenario. (3) Safety and adaptability, Glycyrrhiza uralensis reconciles toxicity and alleviates the potential side effects of Areca nut and Gentiana scabra; Perilla frutescens and Polygonum aviculare take into account the protection of the respiratory and urinary systems, covering the needs of various animals (poultry, aquatic products, ruminants). The traditional Chinese medicine composition of the present invention realizes the synergistic effect of the healthy growth of animals and the prevention and control of diseases through the "digestion promotion - immune activation - pathogen inhibition" trinity mechanism.

[0020] The viable count of Lactobacillus parafarraginis in the compound bacterium agent of the present invention is preferably 300 million - 800 million CFU / g, more preferably 500 million CFU / g, and the viable count of Lactobacillus rhamnosus is 800 million - 1200 million CFU / g, more preferably 1000 million CFU / g. Lactobacillus parafarraginis is Lactobacillus parafarraginis Companilactobacillus alimentarius CICC ® 6284, from the China Center for Industrial Culture Collection, and Lactobacillus rhamnosus is Lactobacillus rhamnosus Lacticaseibacillus rhamnosus CICC ® 25096, from the China Center for Industrial Culture Collection.

[0021] The compound bacterium agent of the present invention can secrete digestive enzymes, improve the feed utilization rate, enhance the metabolic efficiency and nutrient absorption capacity; can be quickly colonized in the intestine, inhibit the proliferation of harmful bacteria (such as Escherichia coli, Salmonella), and at the same time promote the proliferation of beneficial bacteria such as Lactobacillus and Bifidobacterium, reducing the competitive consumption of nutrients; enhance the intestinal barrier and enhance the systemic immune response. The combined use of Lactobacillus parafarraginis and Lactobacillus rhamnosus can form a "nutrition - immunity" dual-core drive, and can promote the absorption of traditional Chinese medicine extracts, significantly improving the comprehensive health level of animals.

[0022] The present invention also provides a preparation method of the above traditional Chinese medicine feed additive, including: crushing Gentiana scabra, Amomum galanga, Isatis indigotica, Carpesium abrotanoides, Areca nut powder and Cinnamomum cassia leaf, adding a eutectic solvent and water, performing ultrasonic extraction, centrifuging, purifying, concentrating and drying to obtain material 1; crushing Piper betle, Coix root, Cimicifuga foetida, Glycyrrhiza uralensis and Perilla frutescens, adding an ethanol solution for ultrasonic extraction, centrifuging, concentrating and drying to obtain material 2; mixing material 1 and material 2 to obtain a traditional Chinese medicine extract, and adding a compound bacterium agent to obtain the traditional Chinese medicine feed additive.

[0023] The extraction solvent in the present invention is a deep eutectic solvent and water. The deep eutectic solvent is preferably composed of choline chloride and isopropanol in a molar ratio of 1:1.5 - 2.5, more preferably 1:2. The weight ratio of the deep eutectic solvent to water is preferably 1 - 3:2 - 6, more preferably 2:5. The temperature of ultrasonic extraction is preferably 45 - 55 °C, more preferably 50 °C, the power is preferably 200 - 300 W, more preferably 250 W, the number of times is preferably 1 - 2 times, more preferably 2 times, and each extraction is preferably for 10 - 20 min, more preferably 15 min. The purification preferably uses AB-8 macroporous adsorption resin, and the purification method is preferably: after passing the supernatant through an AB-8 macroporous adsorption resin column, it is eluted successively with deionized water, 45% - 48% (v / v) ethanol solution, and 74% - 76% (v / v) ethanol solution, and the eluate of the 74% - 76% (v / v) ethanol solution is collected. In the present invention, eluting with water first can elute the deep eutectic solvent (which is soluble in water) adsorbed on the macroporous adsorption resin column, enrich the active ingredients in Material 1, improve the body's resistance, and achieve the effects of promoting growth and preventing plague diseases.

[0024] The volume percentage content of the ethanol solution in the present invention is preferably 60% - 70%, more preferably 65%. The temperature is preferably 50 - 60 °C, more preferably 55 °C, the power is preferably 200 - 300 W, more preferably 250 W, the number of times is preferably 2 - 3 times, more preferably 3 times, and each time is preferably 20 - 30 min, more preferably 25 min.

[0025] The present invention also provides the application of the above-mentioned traditional Chinese medicine feed additive in the preparation of a feed for promoting growth and preventing plague diseases.

[0026] Preferably, the traditional Chinese medicine feed additive is used in the feeds for fish, poultry, and livestock.

[0027] More preferably, the dosage of the traditional Chinese medicine feed additive is preferably 3‰ - 8‰ of the weight of the feed. Pregnant livestock In the present invention, unless otherwise specified, all raw material components are commercially available products well-known to those skilled in the art.

[0028] Next, the technical solutions in the present invention will be clearly and completely described in conjunction with the embodiments in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

[0029] In the following embodiments, unless otherwise specified, they are all conventional methods.

[0030] The materials, reagents, etc. used in the following embodiments, unless otherwise specified, can all be obtained from commercial channels.

[0031] Lactobacillus parafarraginis is Lactobacillus parafarraginis Companilactobacillus alimentarius CICC ® 6284, purchased from China Center of Industrial Culture Collection. Lactobacillus rhamnosus is Lactobacillus rhamnosus Lacticaseibacillus rhamnosus CICC ® 25096, purchased from China Center of Industrial Culture Collection.

[0032] Example 1 Preparation of Traditional Chinese Medicine Feed Additive S1. Weighing Weigh 6 parts of gentian, 4 parts of galangal, 6 parts of folium isatidis, 2.5 parts of carpesium abrotanoides, 4 parts of areca nut, 5 parts of cinnamon leaf, 3 parts of piper betle, 8 parts of coix root, 4 parts of cimicifuga foetida, 12 parts of liquorice and 10 parts of perilla frutescens respectively according to weight parts; S2. Preparation of Material 1 Crush gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut powder and cinnamon leaf and sieve them through a 500-mesh sieve. Add extraction solvent (composed of deep eutectic solvent and water according to a weight ratio of 2:5, and the deep eutectic solvent is composed of choline chloride and isopropanol according to a molar ratio of 1:2) according to a material-liquid ratio of 1:30 g / mL. Ultrasonically extract for 15 min at 50 °C and 250 W, then centrifuge at 4000 r / min for 10 min. Extract the precipitate once again according to the above method, combine the supernatant, pass through an AB-8 macroporous adsorption resin column, and elute successively with deionized water, 46% (v / v) ethanol solution, and 75% (v / v) ethanol solution. Collect the eluate of 75% (v / v) ethanol solution, concentrate and dry to a water content of 4% (w / w) to obtain Material 1; S3. Preparation of Material 2 Crush piper betle, coix root, cimicifuga foetida, liquorice and perilla frutescens, add 65% (v / v) ethanol solution according to a material-liquid ratio of 1:15 g / mL, extract at 55 °C and 250 W for 25 min, then centrifuge at 4000 r / min for 10 min. Extract the precipitate twice again according to the above method, combine the supernatant, concentrate and dry to a water content of 3% (w / w) to obtain Material 2; S4. Preparation of Traditional Chinese Medicine Feed Additive Mix Material 1 and Material 2 to obtain a traditional Chinese medicine extract, add a compound bacterial agent, and stir at 300 r / min for 40 min to obtain a traditional Chinese medicine feed additive; Among them, the compound bacterial agent is composed of Lactobacillus parafarraginis and Lactobacillus rhamnosus. The viable count of Lactobacillus parafarraginis is 500 million CFU / g, and the viable count of Lactobacillus rhamnosus is 1 billion CFU / g; The weight ratio of the traditional Chinese medicine extract to the compound bacterial agent is 10:3.

[0033] Example 2 Preparation of Traditional Chinese Medicine Feed Additive S1. Weighing Weigh 5 parts of gentiana scabra, 3 parts of amomum galanga, 5 parts of folium isatidis, 2 parts of carpesium abrotanoides, 3 parts of areca catechu, 4 parts of cinnamon leaf, 2 parts of piper betle, 6 parts of coix root, 3 parts of cimicifuga foetida, 10 parts of liquorice root and 8 parts of perilla frutescens respectively by weight; S2. Preparation of Material 1 Crush gentiana scabra, amomum galanga, folium isatidis, carpesium abrotanoides, areca catechu powder and cinnamon leaf and pass through a 400-mesh sieve. Add an extraction solvent (composed of a deep eutectic solvent and water in a weight ratio of 1:2, and the deep eutectic solvent is composed of choline chloride and isopropanol in a molar ratio of 1:1.5) according to a material-liquid ratio of 1:25 g / mL. Perform ultrasonic extraction at 45°C and 200 W for 20 min, then centrifuge at 3500 r / min for 15 min, and pass through an AB-8 type macroporous adsorption resin column. Elute successively with deionized water, 45% (v / v) ethanol solution, and 74% (v / v) ethanol solution. Collect the eluate of the 74% (v / v) ethanol solution, concentrate and dry to a water content of 5% (w / w) to obtain Material 1; S3. Preparation of Material 2 Crush piper betle, coix root, cimicifuga foetida, liquorice root and perilla frutescens, and add 60% (v / v) ethanol solution according to a material-liquid ratio of 1:12 g / mL. Extract at 50°C and 200 W for 30 min, then centrifuge at 3500 r / min for 15 min. Extract the precipitate once again according to the above method, combine the supernatant, concentrate and dry to a water content of 4% (w / w) to obtain Material 2; S4. Preparation of Traditional Chinese Medicine Feed Additive Mix Material 1 and Material 2 to obtain a traditional Chinese medicine extract, add a compound bacterial agent, and stir at 250 r / min for 60 min to obtain a traditional Chinese medicine feed additive; Among them, the compound bacterial agent is composed of lactobacillus parafarraginis and lactobacillus rhamnosus. The viable count of lactobacillus parafarraginis is 300 million CFU / g, and the viable count of lactobacillus rhamnosus is 800 million CFU / g; The weight ratio of the traditional Chinese medicine extract to the compound bacterial agent is 12:5.

[0034] Example 3 Preparation of Traditional Chinese Medicine Feed Additive S1. Weighing Weigh 8 parts of gentiana scabra, 5 parts of amomum galanga, 8 parts of folium isatidis, 3 parts of carpesium abrotanoides, 5 parts of areca catechu, 6 parts of cinnamon leaf, 4 parts of piper betle, 10 parts of coix root, 5 parts of cimicifuga foetida, 15 parts of liquorice root and 12 parts of perilla frutescens respectively by weight; S2. Preparation of Material 1 Crush gentiana, galangal, folium isatidis, carpesium abrotanoides, betel nut powder and cinnamon leaf, and sieve them through a 600-mesh sieve. Add an extraction solvent (composed of a deep eutectic solvent and water in a weight ratio of 3:6, and the deep eutectic solvent is composed of choline chloride and isopropanol in a molar ratio of 1:2.5) according to a solid-liquid ratio of 1:40 g / mL. After ultrasonic extraction at 55 °C and 300 W for 10 min, centrifuge at 4500 r / min for 8 min, extract the precipitate once again according to the above method, combine the supernatant, pass it through an AB-8 macroporous adsorption resin column, and elute successively with deionized water, 48% (v / v) ethanol solution, and 76% (v / v) ethanol solution. Collect the eluate of the 76% (v / v) ethanol solution, concentrate and dry it to a water content of 2% (w / w) to obtain Material 1; S3. Preparation of Material 2 Crush betel pepper, coix root, cimicifuga foetida, liquorice and perilla, add 70% (v / v) ethanol solution according to a solid-liquid ratio of 1:18 g / mL, extract at 60 °C and 300 W for 20 min, centrifuge at 4500 r / min for 8 min, extract the precipitate twice again according to the above method, combine the supernatant, concentrate and dry it to a water content of 5% (w / w) to obtain Material 2; S4. Preparation of Chinese herbal medicine feed additive Mix Material 1 and Material 2 to obtain a Chinese herbal medicine extract, add a compound bacterial agent, and stir at 400 r / min for 35 min to obtain a Chinese herbal medicine feed additive; Among them, the compound bacterial agent is composed of Lactobacillus parafarraginis and Lactobacillus rhamnosus, the viable count of Lactobacillus parafarraginis is 800 million CFU / g, and the viable count of Lactobacillus rhamnosus is 1.2 billion CFU / g; The weight ratio of the Chinese herbal medicine extract to the compound bacterial agent is 8:1.

[0035] Example 4 The difference between this Example 1 and Example 1 is that when preparing the Chinese herbal medicine extract, rhizoma iridis is added, and the weight portion of rhizoma iridis is 6 parts, and it is co-extracted with other Chinese herbal medicine raw materials when preparing Material 1.

[0036] Comparative Example 1 The difference between this comparative example and Example 1 is that: the extraction solvent in step S2 is replaced with 75% (v / v) ethanol solution.

[0037] Comparative Example 2 The difference between this comparative example and Example 1 is that: the 65% (v / v) ethanol solution in step S3 is replaced with deionized water.

[0038] Comparative Example 3 The difference between this comparative example and Example 1 is that: the compound bacterial agent is replaced with Lactobacillus parafarraginis with a viable count of 1.5 billion CFU / g.

[0039] Comparative Example 4 The difference between this comparative example and Example 1 is that the composite bacterial agent is replaced with Lactobacillus rhamnosus with a viable count of 1.5 billion CFU / g.

[0040] Comparative Example 5 The difference between this comparative example and Example 1 is that only the Chinese herbal medicine extract is added.

[0041] Comparative Example 6 The difference between this comparative example and Example 4 is that the extraction solvent in step S2 is replaced with a 75% (v / v) ethanol solution.

[0042] Comparative Example 7 The difference between this comparative example and Example 4 is that the 65% (v / v) ethanol solution in step S3 is replaced with deionized water.

[0043] Comparative Example 8 The difference between this comparative example and Example 4 is that the composite bacterial agent is replaced with Lactobacillus parafarraginis with a viable count of 1.5 billion CFU / g.

[0044] Comparative Example 9 The difference between this comparative example and Example 4 is that the composite bacterial agent is replaced with Lactobacillus rhamnosus with a viable count of 1.5 billion CFU / g.

[0045] Comparative Example 10 The difference between this comparative example and Example 4 is that only the Chinese herbal medicine extract is added.

[0046] Test Example 1 Rabbit animal experiment (1) Selection and feeding of animals Select 105 healthy 5-week-old Chinchilla female rabbits with no significant difference in body weight, 15 in each group, and divide them into 7 groups on average: The blank control group is fed a basal diet; The group of Example 1 and the groups of Comparative Examples 1-5 are respectively fed a basal diet containing 5‰ (w / w) of the Chinese herbal medicine feed additive of Example 1 and Comparative Examples 1-5; The basal diet is, by weight percentage: 30% corn, 12% wheat bran, 25% soybean cake, 3% sardine powder, 27.5% alfalfa meal, 2% bone meal and 0.5% salt.

[0047] During the whole animal experiment, according to the conventional rabbit feeding management, such as regularly cleaning and disinfecting the feeding cages, free drinking water, and manually feeding 3 times a day. After one week of adaptive feeding, the formal feeding lasts for 8 weeks.

[0048] (2) Growth performance The body weights of rabbits in each group on an empty stomach at 8:00 am were measured before formal feeding and 8 weeks after formal feeding, respectively. The daily feed intakes were recorded, and the average daily weight gains and feed conversion ratios of each group were calculated. The specific results are shown in Table 1.

[0049] Average daily weight gain = (total body weight after 8 weeks - total body weight before feeding) / (number of test days × number of animals per group); Feed conversion ratio = average daily feed intake / average daily weight gain.

[0050] Table 1 Growth performance of rabbits in each group

[0051] As can be seen from the data in Table 1, after adding the traditional Chinese medicine feed additive, the average daily weight gain of rabbits can be increased, and the feed conversion ratio can be reduced. The effect of Example 1 is the best, indicating that the use of the traditional Chinese medicine additive of the present invention can promote the absorption of nutrients and improve the utilization rate of feed. From the comparison between Example 1 and Comparative Examples 1-2, it can be seen that the use of an insoluble solvent for extraction has an impact on the growth performance of rabbits. From the comparison between Example 1 and Comparative Examples 3-5, it can be seen that there is a synergistic effect between Lactobacillus alimentarius and Lactobacillus rhamnosus. The two used together and added to the feed additive can increase the average daily weight gain and reduce the feed conversion ratio.

[0052] (3)Disease resistance to rabbit hemorrhagic disease After 8 weeks of feeding, the growth status of the Chinchilla female rabbits in each group was observed. In the blank group, one Chinchilla female rabbit had a sudden decrease in appetite, refused to eat, and was listless. In Comparative Example 5, one Chinchilla female rabbit had diarrhea. When the symptoms appeared, they were immediately isolated, and the sick Chinchilla female rabbits were removed. The other Chinchilla rabbits were continued to be fed for 7 days according to the grouping, and all of them were in good growth condition and in a healthy state. Except for the blank group and Comparative Example 5 group, 1 rabbit was randomly removed from each group, that is, 14 Chinchilla female rabbits in each group.

[0053] At 8:00 am on the 8th day, each group of Chinchilla female rabbits was artificially fed according to the method in (1). After 2 hours of feeding, 0.2 mL / rabbit (2LD 50 ) of RHDV was intramuscularly injected to each rabbit according to the method of the invention patent of Patent CN104547242 B. After injection, the feeding was continued according to the method in (1).

[0054] The death situation of rabbits in each group was observed and recorded every day. The dead rabbits were dissected one by one in time to observe the lesions, and the rabbits without lesions were removed. When the death of the whole group stopped, starting from the 5th day, there was no death in each group. The number of dead rabbits in each group per day from the 1st to the 4th day was counted, and the final survival rate was counted. The specific results are shown in Table 2.

[0055] Table 2 Prevention and control effects of each group on hemorrhagic disease

[0056] As can be seen from the data in Table 2, overall, the Chinese herbal medicine feed additives fed in Example 1 and Comparative Examples 1-5 have different degrees of prevention and control effects on rabbit hemorrhagic disease, among which the prevention and control effect of the Example 1 group on the RHDV virus is the most significant. From the comparison between Example 1 and Comparative Examples 1-5, it can be seen that the use of insoluble solvent extraction, the addition of bacterial agents, the compounding of bacterial agents, and the addition of Chinese herbal medicine extracts will all affect the prevention and control effect of rabbits on the RHDV virus. Using the Chinese herbal medicine feed additive obtained by the preparation method of the present invention in feed can improve the body immunity of rabbits and enhance the prevention and control effect on rabbit hemorrhagic disease.

[0057] Test Example 2 Domestic pig animal experiment (1)Selection and feeding of animals Select 105 healthy 45-day-old piglets with no significant difference in body weight, 15 in each group, and divide them into 7 groups on average: The blank control group is fed a basal diet; The Example 4 group and Comparative Examples 6-10 groups are respectively fed a basal diet containing 7‰ (w / w) of the Chinese herbal medicine feed additives of Example 4 and Comparative Examples 6-10.

[0058] The basal diet is as follows by weight percentage: 45% corn, 15% oats, 25% extruded soybean meal, 7% whey powder, 3.8% fish oil, 4% cod fish meal, and 0.2% salt.

[0059] During the whole animal experiment, according to the conventional domestic pig feeding management, such as regularly cleaning and disinfecting the feeding environment, free drinking water, and manually feeding 3 times a day (the feeding amount is adjusted according to the pig growth cycle). After one week of adaptive feeding, the formal feeding lasts for 60 days.

[0060] (2)Growth performance Measure the empty stomach weight of each group of piglets at 8 am before the formal feeding and 60 days after the formal feeding respectively, record the daily feed intake, and calculate the average daily weight gain and feed to gain ratio of each group. The specific results are shown in Table 3.

[0061] Average daily weight gain = (total weight after 60 days - total weight before feeding) / (test days × number of animals in each group); Feed to gain ratio = average daily feed intake / average daily weight gain.

[0062] Table 3 Growth performance of each group of domestic pigs

[0063] As can be seen from the data in Table 3, overall, compared with the blank group, other groups can all increase the average daily weight gain of piglets and reduce the feed-to-weight ratio. The effect of Example 4 is the best, indicating that the Chinese medicine additive of the present invention can improve the growth rate of piglets. From the comparison between Example 4 and Comparative Examples 6-7, it can be seen that the extraction with insoluble solvents has an impact on the growth performance of piglets. From the comparison between Example 4 and Comparative Examples 8-10, it can be known that there is a synergistic effect between Lactobacillus alimentarius and Lactobacillus rhamnosus. The combination of the two added to the feed additive can increase the average daily weight gain, reduce the feed-to-weight ratio, and promote the growth of piglets.

[0064] (3)Disease resistance to classical swine fever virus CSFV After 60 days of feeding, the growth status of piglets in each group was observed. One piglet in Comparative Example 7 had diarrhea, and one piglet in Comparative Example 10 had vomiting. When the symptoms appeared, the sick piglets were immediately isolated and removed. The other piglets were continued to be fed according to the grouping for 7 days, and all of them were in good growth condition and in a healthy state. Except for the groups of Comparative Example 7 and Comparative Example 10, 1 piglet was randomly removed from each group, that is, 14 piglets in each group.

[0065] At 8:00 in the morning on the 8th day, each group of piglets was artificially fed according to the method in (1). After 2 hours of feeding, the highly virulent strain SM of CSFV (5×10 3.61 TCID 50 / mL) was used to inoculate the piglets in each group by intramuscular injection in the buttocks. After inoculation, the feeding continued according to the method in (1).

[0066] The status and death of piglets in each group were observed and recorded every day. If symptoms such as high fever, listlessness, loss of appetite, redness of the conjunctiva, and purple-red spots or bleeding points on the skin appeared, the piglet was immediately isolated. When the death of the whole group stopped, the final survival rate was counted. The specific results are shown in Table 4.

[0067] Table 4 Prevention and control effects of each group on classical swine fever virus CSFV

[0068] As can be seen from the data in Table 4, overall, feeding the Chinese medicine feed additives of Example 4 and Comparative Examples 6-10 has different degrees of prevention and control effects on classical swine fever. Among them, the prevention and control effect of the group of Example 4 on CSFV virus is the most significant. From the comparison between Example 4 and Comparative Examples 6-10, it can be seen that the extraction with insoluble solvents, the addition of bacterial agents, the compounding of bacterial agents, and the addition of Chinese medicine extracts will all affect the prevention and control effect of piglets on classical swine fever. Using the Chinese medicine feed additive obtained by the preparation method of the present invention in feed can improve the body immunity of piglets and has a significant effect on preventing classical swine fever CSFV.

[0069] In summary, the Chinese medicine extract in the Chinese medicine feed additive of the present invention can improve the immunity of animals, and the compound bacterial agent can promote the absorption of the Chinese medicine extract and other nutritional components by the body, improve the bioavailability, and thus achieve the effects of promoting growth and preventing epidemic diseases.

[0070] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A traditional Chinese medicine feed additive for promoting growth and preventing plague diseases, characterized in that, It comprises the following raw materials in parts by weight: 8 - 12 parts of Chinese medicine extract and 1 - 5 parts of compound bacterial agent; the Chinese medicine extract is obtained by extracting gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut, cinnamon leaf, betel pepper, coix root, cimicifuga foetida, liquorice and perilla; the compound bacterial agent includes lactobacillus parafarraginis and lactobacillus rhamnosus.

2. The Chinese medicine feed additive according to claim 1, characterized in that, The weight ratio of gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut, cinnamon leaf, betel pepper, coix root, cimicifuga foetida, liquorice and perilla is (5 - 8):(3 - 5):(5 - 8):(2 - 3):(3 - 5):(4 - 6):(2 - 4):(6 - 10):(3 - 5):(10 - 15):(8 - 12).

3. The traditional Chinese medicine feed additive according to claim 1, characterized in that, In the compound bacterial agent, the viable count of lactobacillus parafarraginis is 300 million - 800 million CFU / g, and the viable count of lactobacillus rhamnosus is 800 million - 1200 million CFU / g.

4. The preparation method of the traditional Chinese medicine feed additive according to any one of claims 1-3, characterized in that, It includes: Crush gentian, galangal, folium isatidis, carpesium abrotanoides, areca nut powder and cinnamon leaf, add an extraction solvent, carry out ultrasonic extraction, then centrifuge, purify, concentrate and dry to obtain Material 1; Crush betel pepper, coix root, cimicifuga foetida, liquorice and perilla, add an ethanol solution for ultrasonic extraction, centrifuge, concentrate and dry to obtain Material 2; Mix Material 1 and Material 2 to obtain the Chinese medicine extract, and add the compound bacterial agent to obtain the Chinese medicine feed additive.

5. The preparation method according to claim 4, characterized in that, The extraction solvent is a deep eutectic solvent and water, the deep eutectic solvent is composed of choline chloride and isopropanol according to a molar ratio of 1:1.5 - 2.5, and the weight ratio of the deep eutectic solvent to water is 1 - 3:2 - 6.

6. The preparation method according to claim 4, characterized in that, The temperature of the ultrasonic extraction for obtaining Material 1 is 45 - 55°C, the power is 200 - 300W, the number of times is 1 - 2 times, and each extraction is 10 - 20min.

7. The preparation method according to claim 4, characterized in that, In the ultrasonic extraction with the ethanol solution, the volume percentage content of the ethanol solution is 60% - 70%, the temperature is 50 - 60°C, the power is 200 - 300W, the number of times is 2 - 3 times, and each time is 20 - 30min.

8. Use of the Chinese medicine feed additive according to claim 1 in the preparation of a feed for promoting growth and preventing epidemic diseases.

9. The application according to claim 9, wherein The Chinese medicine feed additive is used for the feeds of fish, poultry and livestock.

10. The application according to claim 8 or 9, characterized in that, The dosage of the Chinese medicine feed additive is 3‰ - 8‰ of the feed weight.

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