Feed for improving growth performance of Tibetan sheep as well as preparation method and application of feed

Abstract

The invention relates to the technical field of feeds, in particular to a feed for improving the growth performance of Tibetan sheep and a preparation method and application thereof. The feed comprises the following components in parts by mass: 40-50 parts of corn, 5-10 parts of soybean meal, 1-5 parts of safflower seed meal, 1-5 parts of corn germ meal, 1-5 parts of cottonseed meal, 0.5-1 part of rapeseed meal, 5-10 parts of alfalfa hay, 3-8 parts of corn straw, 3-8 parts of sunflower peel, 1-5 parts of soybean hull, 8-15 parts of corn bran, 0.5-1 part of table salt, 1-5 parts of molasses, 1-2 parts of calcium hydrophosphate, 0.5-1.5 parts of sodium bicarbonate, 0.1-0.5 part of yeast cell wall polysaccharide and 0.1-1 part of premix. The feed can improve the average daily gain of the Tibetan sheep, reduce the feed-gain ratio, and improve the immunity of the Tibetan sheep by changing the gastrointestinal environment and the liver metabolic pathway.

Description

Technical Field

[0001] The present invention relates to the technical field of feeds, and particularly relates to a feed for improving the growth performance of Tibetan sheep, a preparation method thereof, and an application thereof. Background Art

[0002] Yeast is a natural fermentation product, and its derivatives, especially yeast cell wall. The yeast cell wall (Yeast Cell Wall, YCW) is extracted from Saccharomyces cerevisiae and contains polysaccharides such as mannan and β-glucan, belonging to immunomodulatory compounds. It has been found that yeast cell wall has the effects of improving animal production performance, adsorbing pathogenic microorganisms in the digestive tract, adjusting beneficial colonies in the digestive tract, maintaining intestinal health, regulating the body's immune function, enhancing the body's antioxidant capacity, etc. As an important part of yeast-based biological feed raw materials, as a safe and efficient feed additive, it is widely used in the breeding industry.

[0003] However, there are still certain defects in the preparation of feeds in combination with yeast cell wall in the prior art: for example, the types of yeast selected during the fermentation process, the extraction process, and the types of animals studied, etc., will all affect the physicochemical properties and functions of yeast cell wall polysaccharides, ultimately resulting in different effects; in addition, yeast cell wall polysaccharides may become allergens, or cause nutritional imbalance in animals due to improper dosage, and due to the bitter taste of yeast, the palatability is poor, which may also affect the feed intake of animals. Therefore, when using feeds containing yeast cell wall, it is necessary to comprehensively consider its advantages and disadvantages. Summary of the Invention

[0004] The purpose of the present invention is to provide a feed for improving the growth performance of Tibetan sheep, a preparation method thereof, and an application thereof.

[0005] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0006] The present invention provides a feed for improving the growth performance of Tibetan sheep, comprising the following components in parts by mass:

[0007] 40 - 50 parts of corn, 5 - 10 parts of soybean meal, 1 - 5 parts of safflower seed meal, 1 - 5 parts of corn germ meal, 1 - 5 parts of cottonseed meal, 0.5 - 1 part of rapeseed meal, 5 - 10 parts of alfalfa hay, 3 - 8 parts of corn straw, 3 - 8 parts of sunflower hulls, 1 - 5 parts of soybean hulls, 8 - 15 parts of corn bran, 0.5 - 1 part of salt, 1 - 5 parts of molasses, 1 - 2 parts of calcium hydrogen phosphate, 0.5 - 1.5 parts of sodium bicarbonate, 0.1 - 0.5 part of yeast cell wall polysaccharide, 0.1 - 1 part of premix.

[0008] Preferably, per kilogram of the premix contains 20 - 30 mg of iron, 30 - 50 mg of manganese, 30 - 50 mg of zinc, 5 - 10 mg of copper, 0.1 - 0.5 mg of iodine, 0.1 - 0.5 mg of selenium, 0.05 - 0.15 mg of cobalt, 900 - 1000 IU of vitamin A, 100 - 120 IU of vitamin D, and 15 - 25 IU of vitamin E.

[0009] Preferably, the crude protein content in the feed is ≥9%, the neutral detergent fiber content is ≥27%, the acid detergent fiber content is ≥17%, the crude ash content is ≥4%, the calcium content is ≥0.8%, and the phosphorus content is ≥0.4%.

[0010] The present invention also provides a method for preparing a feed for improving the growth performance of Tibetan sheep, comprising the following steps:

[0011] (1) Weigh each component according to the mass parts in the feed.

[0012] (2) Crush each component to a particle size of ≤2 mm and granulate to obtain the feed.

[0013] Preferably, the diameter of the prepared feed is 3 - 4 mm.

[0014] The present invention also provides the application of the feed in improving the growth performance of Tibetan sheep.

[0015] The present invention also provides the application of the feed in promoting the digestive function of Tibetan sheep. The feed can increase the villus length of the duodenum and jejunum of Tibetan sheep and reduce the crypt depth of the ileum.

[0016] The present invention also provides the application of the feed in improving the gastrointestinal microbial composition of Tibetan sheep, promoting the content of beneficial microorganisms in the gastrointestinal tract of Tibetan sheep and reducing the abundance of harmful microorganisms.

[0017] The present invention also provides the application of the feed in regulating the liver metabolic function of Tibetan sheep.

[0018] The present invention also provides the application of the feed in improving the immune ability of Tibetan sheep.

[0019] The present invention provides a feed for improving the growth performance of Tibetan sheep. The feed of the present invention comprises the following components in mass parts: 40 - 50 parts of corn, 5 - 10 parts of soybean meal, 1 - 5 parts of safflower seed meal, 1 - 5 parts of corn germ meal, 1 - 5 parts of cottonseed meal, 0.5 - 1 part of rapeseed meal, 5 - 10 parts of alfalfa hay, 3 - 8 parts of corn straw, 3 - 8 parts of sunflower hull, 1 - 5 parts of soybean hull, 8 - 15 parts of corn hull, 0.5 - 1 part of salt, 1 - 5 parts of molasses, 1 - 2 parts of monocalcium phosphate, 0.5 - 1.5 parts of sodium bicarbonate, and 0.1 - 0.5 part of yeast cell wall polysaccharide. The feed of the present invention has the following beneficial effects:

[0020] (1) It can increase the average daily gain of Tibetan sheep while reducing the feed-to-gain ratio.

[0021] (2) It can enhance the intestinal barrier of Tibetan sheep and improve its digestive function by increasing the height of intestinal villi and the ratio of villus to crypt in Tibetan sheep.

[0022] (3) It can promote the increase of beneficial microorganisms (such as Rikenellaceae_RC9_gut_group and Ruminococcus in the gastrointestinal microbiota of Tibetan sheep), while reducing the abundance of harmful microorganisms. In addition, the feed of the present invention may also improve the health of its gastrointestinal tract, enhance the liver metabolism performance, and further improve the immunity of Tibetan sheep by participating in pathways such as lipid metabolism, bile acid metabolism, and amino acid metabolism in the liver of Tibetan sheep. Description of the Drawings

[0023] Figure 1 Shows the effects of different feeds on the pH value of rumen contents in Tibetan sheep;

[0024] Figure 2 Shows the effects of different feeds on the total volatile acid content of rumen contents in Tibetan sheep;

[0025] Figure 3 Shows the effects of different feeds on the acetic acid content of rumen contents in Tibetan sheep;

[0026] Figure 4 Shows the effects of different feeds on the propionic acid content of rumen contents in Tibetan sheep;

[0027] Figure 5 Shows the effects of different feeds on the butyric acid content of rumen contents in Tibetan sheep;

[0028] Figure 6 Shows the effects of different feeds on the ammonia nitrogen content of rumen contents in Tibetan sheep;

[0029] Figure 7 Shows the effects of different feeds on the differences in rumen microbial composition in Tibetan sheep (the upper figure shows the differences at the phylum level of bacteria, and the lower figure shows the differences at the genus level of bacteria);

[0030] Figure 8 Shows the effects of different feeds on the differences in colonic microbial composition in Tibetan sheep (the upper figure shows the differences at the phylum level of bacteria, and the lower figure shows the differences at the genus level of bacteria);

[0031] Figure 9 Shows the effects of different feeds on the enrichment of liver metabolite pathways in Tibetan sheep (positive ion mode);

[0032] Figure 10 Shows the effects of different feeds on the enrichment of liver metabolite pathways in Tibetan sheep (negative ion mode). Detailed Embodiments

[0033] The present invention provides a feed for improving the growth performance of Tibetan sheep, comprising the following components in parts by mass:

[0034] Corn 40 - 50 parts, preferably 42 - 48 parts, more preferably 45 parts;

[0035] Soybean meal 5 - 10 parts, preferably 6 - 9 parts, more preferably 7.5 parts;

[0036] Safflower seed meal 1 - 5 parts, preferably 2 - 4 parts, more preferably 3 parts;

[0037] Corn germ meal 1 - 5 parts, preferably 2 - 4 parts, more preferably 3 parts;

[0038] Cottonseed meal 1 - 5 parts, preferably 2 - 4 parts, more preferably 3 parts;

[0039] Rapeseed meal 0.5 - 1 part, preferably 0.6 - 0.9 part, more preferably 0.75 part;

[0040] Alfalfa hay 5 - 10 parts, preferably 6 - 9 parts, more preferably 7.5 parts;

[0041] Corn straw 3 - 8 parts, preferably 4 - 7 parts, more preferably 6.5 parts;

[0042] Sunflower hull 3 - 8 parts, preferably 4 - 7 parts, more preferably 6.5 parts;

[0043] Soybean hull 1 - 5 parts, preferably 2 - 4 parts, more preferably 3 parts;

[0044] Corn hull 8 - 15 parts, preferably 10 - 13 parts, more preferably 12 parts;

[0045] Table salt 0.5 - 1 part, preferably 0.6 - 0.9 part, more preferably 0.75 part;

[0046] Molasses 1 - 5 parts, preferably 2 - 4 parts, more preferably 3 parts;

[0047] Calcium hydrogen phosphate 1 - 2 parts, preferably 1.2 - 1.8 parts, more preferably 1.5 parts;

[0048] Sodium bicarbonate 0.5 - 1.5 parts, preferably 0.7 - 1.3 parts, more preferably 1.0 part;

[0049] Yeast cell wall polysaccharide 0.1 - 0.5 part, preferably 0.2 - 0.4 part, more preferably 0.3 part;

[0050] Premix 0.1 - 1 part, preferably 0.2 - 0.8 part, more preferably 0.5 part.

[0051] In the present invention, each kilogram of the premix contains 20 - 30 mg of iron, preferably 22 - 28 mg, and more preferably 25 mg;

[0052] 30 - 50 mg of manganese, preferably 35 - 45 mg, and more preferably 40 mg;

[0053] 30 - 50 mg of zinc, preferably 35 - 45 mg, and more preferably 40 mg;

[0054] 5 - 10 mg of copper, preferably 6 - 9 mg, and more preferably 7.5 mg;

[0055] 0.1 - 0.5 mg of iodine, preferably 0.2 - 0.4 mg, and more preferably 0.3 mg;

[0056] 0.1 - 0.5 mg of selenium, preferably 0.2 - 0.4 mg, and more preferably 0.3 mg;

[0057] 0.05 - 0.15 mg of cobalt, preferably 0.08 - 0.12 mg, and more preferably 0.1 mg;

[0058] 900 - 1000 IU of vitamin A, preferably 920 - 980 IU, and more preferably 950 IU;

[0059] 100 - 120 IU of vitamin D, preferably 105 - 115 IU, and more preferably 110 IU;

[0060] 15 - 25 IU of vitamin E, preferably 17 - 23 IU, and more preferably 20 IU.

[0061] In the present invention, the crude protein content in the feed is ≥ 9%, the neutral detergent fiber content is ≥ 27%, the acid detergent fiber content is ≥ 17%, the crude ash content is ≥ 4%, the calcium content is ≥ 0.8%, and the phosphorus content is ≥ 0.4%.

[0062] The present invention also provides a preparation method of a feed for improving the growth performance of Tibetan sheep, comprising the following steps:

[0063] (1) Weigh each component according to the mass parts in the feed;

[0064] (2) Crush each component to a particle size ≤ 2 mm and granulate to obtain the feed.

[0065] The preferred particle size for crushing corn, soybean meal, safflower seed meal, corn germ meal, cottonseed meal, rapeseed meal and salt is 0.5 mm;

[0066] The particle size of the crushed alfalfa hay, corn straw, sunflower husk, soybean hull and corn bran is preferably 1 - 2 mm, more preferably 1.5 mm.

[0067] In the present invention, the prepared feed is in the form of pellets, which are cylindrical, with a diameter of 3 - 4 mm, preferably 3.5 mm; and a column height of 1 - 2 cm, preferably 1.5 cm.

[0068] The present invention also provides the application of the said feed in improving the growth performance of Tibetan sheep.

[0069] The present invention also provides the application of the said feed in promoting the digestive function of Tibetan sheep. The feed can increase the villus length of the duodenum and jejunum of Tibetan sheep and reduce the crypt depth of the ileum.

[0070] The present invention also provides the application of the said feed in improving the gastrointestinal microbial composition of Tibetan sheep, promoting the content of beneficial microorganisms in the gastrointestinal tract of Tibetan sheep and reducing the abundance of harmful microorganisms.

[0071] The present invention also provides the application of the said feed in regulating the liver metabolic function of Tibetan sheep.

[0072] The present invention also provides the application of the said feed in enhancing the immune ability of Tibetan sheep.

[0073] The following combines examples to elaborate on the solutions provided by the present invention in detail, but they should not be construed as limiting the protection scope of the present invention.

[0074] The yeast cell wall polysaccharide described in the embodiments of the present invention is purchased from Angel Yeast Co., Ltd. (Hubei Province, China).

[0075] The premix described in the embodiments of the present invention is purchased from Gansu Runmu Bio - engineering Co., Ltd. Each kilogram of the premix contains 25 mg of iron, 40 mg of manganese, 40 mg of zinc, 8 mg of copper, 0.3 mg of iodine, 0.2 mg of selenium, 0.1 mg of cobalt, 940 IU of vitamin A, 111 IU of vitamin D and 20 IU of vitamin E.

[0076] The Tibetan sheep in the experimental examples of the present invention are from the characteristic breeding variety in Kecai Town, Xiahe County, Gansu Province, namely Kecai Tibetan sheep.

[0077] Example 1

[0078] Weigh 46.21 kg of corn, 7.65 kg of soybean meal, 3.5 kg of safflower seed meal, 3.0 kg of corn germ meal, 2.5 kg of cottonseed meal, 0.82 kg of rapeseed meal, 7.0 kg of alfalfa hay, 5.0 kg of corn straw, 5.0 kg of sunflower hulls, 5.0 kg of soybean hulls, 10 kg of corn bran, 0.68 kg of salt, 3.0 kg of molasses, 1.14 kg of calcium hydrogen phosphate, 1.0 kg of calcium bicarbonate, 0.3 kg of yeast cell wall polysaccharide, and 0.5 kg of premix. Crush the corn, soybean meal, safflower seed meal, corn germ meal, cottonseed meal, rapeseed meal, and salt to a particle size of 0.5 mm, and crush the alfalfa hay, corn straw, sunflower hulls, soybean hulls, and corn bran to 1.5 mm. Then mix the crushed components with the other components to obtain a mixture, and granulate the mixture to obtain feed.

[0079] The obtained feed is cylindrical, with a diameter of 3.5 mm and a column height of 1.2 cm.

[0080] Example 2

[0081] Weigh 40 kg of corn, 10 kg of soybean meal, 2.5 kg of safflower seed meal, 5.0 kg of corn germ meal, 1.0 kg of cottonseed meal, 1.0 kg of rapeseed meal, 5.0 kg of alfalfa hay, 8.0 kg of corn straw, 6.0 kg of sunflower hulls, 3.0 kg of soybean hulls, 8.0 kg of corn bran, 0.8 kg of salt, 2.4 kg of molasses, 2.0 kg of calcium hydrogen phosphate, 0.6 kg of calcium bicarbonate, 0.4 kg of yeast cell wall polysaccharide, and 0.3 kg of premix. Crush the corn, soybean meal, safflower seed meal, corn germ meal, cottonseed meal, rapeseed meal, and salt to a particle size of 0.5 mm, and crush the alfalfa hay, corn straw, sunflower hulls, soybean hulls, and corn bran to 1.5 mm. Then mix the crushed components with the other components to obtain a mixture, and granulate the mixture to obtain feed.

[0082] The obtained feed is cylindrical, with a diameter of 3.5 mm and a column height of 1.2 cm.

[0083] Example 3

[0084] Weigh 50 kg of corn, 8.0 kg of soybean meal, 5.0 kg of safflower seed meal, 1.5 kg of corn germ meal, 3.0 kg of cottonseed meal, 0.6 kg of rapeseed meal, 10.0 kg of alfalfa hay, 3.0 kg of corn straw, 8.0 kg of sunflower hulls, 2.0 kg of soybean hulls, 10.0 kg of corn bran, 0.5 kg of salt, 3.5 kg of molasses, 1.8 kg of calcium hydrogen phosphate, 1.2 kg of calcium bicarbonate, 0.2 kg of yeast cell wall polysaccharide, and 0.8 kg of premix. Crush the corn, soybean meal, safflower seed meal, corn germ meal, cottonseed meal, rapeseed meal, and salt to a particle size of 0.5 mm. After crushing the alfalfa hay, corn straw, sunflower hulls, soybean hulls, and corn bran to 1.5 mm, mix the crushed components with the other components to obtain a mixture. Granulate the mixture to obtain the feed.

[0085] The obtained feed is cylindrical, with a diameter of 3.5 mm and a column height of 1.2 cm.

[0086] Control Example 1

[0087] Set up Control Example 1 according to the scheme of Example 1. Different from Example 1, yeast cell wall polysaccharide is not added in Control Example 1.

[0088] Experimental Example 1

[0089] Detect the nutrient content in the feeds of Example 1 and Control Example 1. The results are shown in Table 1.

[0090] Table 1 Nutrient content in different feeds

[0091]

[0092]

[0093] Experimental Example 2

[0094] Thirty Tibetan sheep with good homogeneity are randomly divided into 2 groups, with 5 replicates in each group and 3 Tibetan sheep in each replicate. The Tibetan sheep in the control group (CON) are fed the feed of Control Example 1, and the Tibetan sheep in the experimental group (YCW) are fed the feed of Example 1.

[0095] After a 14-day pre-trial period (the pre-trial period is to allow animals to adapt to various conditions and environments of the experiment as soon as possible. All operations are the same as those in the formal trial, except that samples and data are not collected), a formal trial period of 84 days is carried out. During the experiment, Tibetan sheep are fed in pens in units of replicates, fed twice a day (at 09:00 in the morning and 17:00 in the afternoon), and provided with free drinking water. After the experiment, 6 Tibetan sheep are randomly selected from each group for slaughter. Weighing is carried out before morning feeding on the 0th, 28th, 56th, and 84th days at the beginning of the formal trial period. The average daily gain is calculated using the weight difference of Tibetan sheep before and after the experiment. The feeding amount of each sheep is recorded every day, and the remaining amount of the feed is collected and weighed before morning feeding the next day to calculate the daily feed intake, and the feed-to-gain ratio is calculated. The results are shown in Table 2.

[0096] Table 2 Effects of Different Feeds on the Growth Performance of Tibetan Sheep

[0097] Index CON YCW Average daily feed intake (kg / d) 1.68±0.13 1.71±0.23 Average daily weight gain (g / d) <![CDATA[211.76±47.79 b > <![CDATA[256.22±51.65 a > Feed conversion ratio <![CDATA[7.88±1.70 a > <![CDATA[6.97±1.42 b >

[0098] Note: Different lowercase letters with superscripts indicate significant differences (P < 0.05), while the same letters or no letters indicate no significant differences (P > 0.05).

[0099] As can be seen from Table 1, after feeding the feed of Example 1, the daily gain of Tibetan sheep was significantly increased compared with that of the feed of Comparative Example 1 (P < 0.05), and the feed-to-gain ratio was significantly decreased (P < 0.05).

[0100] Experimental Example 3

[0101] After the 84-day formal feeding trial ended, Tibetan sheep in different groups were slaughtered and sampled. The duodenum, jejunum, and ileum segments were quickly taken, washed clean, fixed in 4% paraformaldehyde, made into paraffin sections, and then HE stained for histological morphological analysis. After feeding different feeds, the intestinal villus length, crypt depth of Tibetan sheep were measured, and the villus-crypt ratio was calculated. The obtained experimental data were expressed as mean ± standard deviation, and T-test analysis was performed using SPSS 22.0 analysis software. The results are shown in Table 3.

[0102] Table 3 Effects of Different Feeds on the Intestinal Morphological Structure of Tibetan Sheep

[0103]

[0104] Note: Different lowercase letters with superscripts indicate significant differences (P < 0.05), while the same letters or no letters indicate no significant differences (P > 0.05).

[0105] As can be seen from Table 3, after feeding the feed of Example 1, compared with feeding the feed of Comparative Example 1, it could significantly increase the villus length of the duodenum and jejunum of Tibetan sheep (P < 0.05), decrease the crypt depth of the ileum (P < 0.05), and increase the villus-crypt ratio of the duodenum and ileum (P < 0.05).

[0106] Experimental Example 4

[0107] After the 84-day formal feeding trial ended, Tibetan sheep in different groups were slaughtered and sampled. The pH value of rumen contents was quickly measured (PB-10 portable pH meter, Shenhua Biotechnology Co., Ltd., Beijing, Figure 1 ), and at the same time, samples of rumen and colon contents were collected, quickly frozen in liquid nitrogen and then transferred to -80 °C for storage, for the determination of volatile fatty acids in the gastrointestinal tract (6890N gas chromatograph, Agilent, USA, Figures 2 - 6 ), and the sequencing analysis of the gastrointestinal microbial composition (V3-V4 region, Illumina NovaSeq platform, Beijing Novogene Bioinformatics Technology Co., Ltd., Figures 7 - 8 ). Liver tissue was quickly taken, frozen in liquid nitrogen and then transferred to -80 °C for storage, for subsequent liver metabolomics analysis (LC-MS / MS, Beijing Novogene Bioinformatics Technology Co., Ltd., Figures 9 - 10 ). The obtained experimental data were expressed as mean ± standard deviation, and T-test analysis was performed using SPSS 22.0 analysis software.

[0108] Figures 1 - 6 The results showed that the feed of Example 1 could significantly increase the propionic acid content in the rumen (P < 0.05) compared with the feed of Comparative Example 1, and extremely significantly increase the ammonia nitrogen content (P < 0.01). ** indicates significant difference (P < 0.05), and *** indicates extremely significant difference (P < 0.01).

[0109] Figures 7 - 8 The results showed that at the phylum level, the relative abundance of Firmicutes in the rumen of CON was significantly higher than that in YCW (P < 0.05); the relative abundances of Desulfobacterota and Synergistota in the rumen were significantly decreased in the YCW group (P < 0.05); the relative abundance of Desulfobacterota in the colon was significantly decreased in the YCW group (P < 0.05); at the genus level, the relative abundance of Prevotella in the rumen was significantly increased in the YCW group (P < 0.05), but the relative abundances of Rikenellaceae_RC9_gut_group, UCG-002, NK4A214_group, and Eubacterium_nodatum_group were significantly decreased (P < 0.05); the relative abundances of Rikenellaceae_RC9_gut_group and Ruminococcus in the colon were significantly increased (P < 0.05), and the relative abundance of UCG-002 was significantly decreased (P < 0.05).

[0110] Figures 9 - 10 It was shown that in the positive ion mode, the differential metabolites were significantly enriched in oxidative phosphorylation, AMPK signaling pathway, bile secretion, and arginine biosynthesis pathways, while in the negative ion mode, the differential metabolites were significantly enriched in glycerolipid metabolism, gap junction, fat digestion and absorption, phospholipase D signaling pathway, vitamin digestion and absorption, and glycolysis / gluconeogenesis pathways (P < 0.05).

[0111] As can be seen from the above embodiments, the present invention provides a feed for improving the growth performance of Tibetan sheep. Feeding Tibetan sheep with the feed prepared according to the components of the present invention can increase the average daily gain of Tibetan sheep and reduce the feed-to-gain ratio at the same time. It can increase the height of small intestinal villi and the ratio of villus to crypt of Tibetan sheep to enhance the intestinal barrier of Tibetan sheep and improve its digestive function. It can promote the increase of beneficial microorganisms in the gastrointestinal microflora of Tibetan sheep and reduce the abundance of harmful microorganisms at the same time. In addition, the feed of the present invention may also improve its gastrointestinal health, enhance the liver metabolic performance, and further improve the immunity of Tibetan sheep by participating in pathways such as liver lipid metabolism, bile acid metabolism, and amino acid metabolism of Tibetan sheep.

[0112] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A feed for improving the growth performance of Tibetan sheep, characterized in that, It comprises components in the following parts by mass: 40 - 50 parts of corn, 5 - 10 parts of soybean meal, 1 - 5 parts of safflower seed meal, 1 - 5 parts of corn germ meal, 1 - 5 parts of cottonseed meal, 0.5 - 1 part of rapeseed meal, 5 - 10 parts of alfalfa hay, 3 - 8 parts of corn straw, 3 - 8 parts of sunflower hull, 1 - 5 parts of soybean hull, 8 - 15 parts of corn hull, 0.5 - 1 part of salt, 1 - 5 parts of molasses, 1 - 2 parts of calcium hydrogen phosphate, 0.5 - 1.5 parts of sodium bicarbonate, 0.1 - 0.5 part of yeast cell wall polysaccharide, 0.1 - 1 part of premix.

2. The feed according to claim 1, characterized in that, Each kilogram of the premix contains 20 - 30 mg of iron, 30 - 50 mg of manganese, 30 - 50 mg of zinc, 5 - 10 mg of copper, 0.1 - 0.5 mg of iodine, 0.1 - 0.5 mg of selenium, 0.05 - 0.15 mg of cobalt, 900 - 1000 IU of vitamin A, 100 - 120 IU of vitamin D, and 15 - 25 IU of vitamin E.

3. The feed according to claim 1 or 2, characterized in that, The crude protein content in the feed is ≥9%, the content of neutral detergent fiber is ≥27%, the content of acid detergent fiber is ≥17%, the content of crude ash is ≥4%, the content of calcium is ≥0.8%, and the content of phosphorus is ≥0.4%.

4. A preparation method of a feed for improving the growth performance of Tibetan sheep, characterized in that, It includes the following steps: (1) Weigh each component according to the parts by mass in the feed described in any one of claims 1 - 3; (2) Crush each component to a particle size of ≤2 mm and granulate to obtain the feed.

5. The preparation method according to claim 4, characterized in that, The diameter of the prepared feed is 3 - 4 mm.

6. Use of the feed described in any one of claims 1 - 3 in improving the growth performance of Tibetan sheep.

7. Use of the feed according to any one of claims 1 to 3 in promoting the digestive function of Tibetan sheep, characterized in that, The feed can increase the villus length of the duodenum and jejunum of Tibetan sheep and reduce the crypt depth of the ileum.

8. Use of the feed according to any one of claims 1 to 3 in improving the gastrointestinal microbial composition of Tibetan sheep, characterized in that, Promote the content of beneficial microorganisms in the gastrointestinal tract of Tibetan sheep and reduce the abundance of harmful microorganisms.

9. Use of the feed described in any one of claims 1 - 3 in regulating the liver metabolic function of Tibetan sheep.

10. Use of the feed described in any one of claims 1 - 3 in improving the immune ability of Tibetan sheep.

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