Compound amino acid whitmania pigra whitman polypeptide food and preparation method thereof

Abstract

The invention relates to the technical field of food, in particular to compound amino acid gilt-edged leech polypeptide food and a preparation method thereof. According to the compound amino acid gilt-edged leech polypeptide food, gilt-edged leech polypeptide and amino acid serve as main drugs and serve as a core body, the core body is wrapped with a first skin layer, and radix puerariae powder, Chinese yam powder, onion powder and a freeze-drying agent serve as first auxiliary materials of the first skin layer; the first skin layer is wrapped by a second skin layer, the second skin layer takes tannic acid powder, edible starch and a preservative as second auxiliary materials, and the mass ratio of the tannic acid powder to the gilt-edge leech polypeptide is (1-5): (1-5), so that the gilt-edge leech polypeptide in the polypeptide food is not decomposed in the stomach under the protection effect of the tannic acid powder, and the gilt-edge leech polypeptide is not decomposed in the stomach; the gilt-edged leech polypeptide plays a drug effect after reaching the small intestine, so that the bioavailability is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of food processing, and particularly relates to a compound amino acid golden-ringed leech polypeptide food and a preparation method thereof. Background Art

[0002] Every year, up to 15 million people worldwide die from cardiovascular and cerebrovascular diseases, ranking first among all causes of death. Cardiovascular and cerebrovascular diseases and their complications have become the number one killer threatening human life. These diseases are mostly related to thrombosis, blood coagulation, and blood viscosity. Thrombosis and blood lipid are common diseases among middle-aged and elderly people over 50 years old, seriously threatening human health. Most clinical treatments rely strictly on drugs. Existing oral thrombolytic products have many disadvantages such as poor performance, large side effects of chemical thrombolytic drugs, incomplete thrombolysis, and large side effects on the body. Moreover, the side effects of various lipid-lowering and cholesterol-lowering drugs limit the scope of treatment and application.

[0003] Hirudin polypeptide is a natural substance with active ingredients extracted from the saliva or fresh extract of the blood-sucking leech Whitmania pigra Whitman. It is a small molecule protein (polypeptide) composed of 65-66 amino acids, also known as Whitmania pigra polypeptide. The molecular weight of natural Whitmania pigra polypeptide is only 7000 Daltons, which is extremely soluble in water and physiological saline and is very stable at room temperature. Simple temperature increase (100°C water bath) or pH change (1-13) does not affect its activity. Whitmania pigra polypeptide is the most potent natural specific inhibitor of thrombin discovered so far. It is not destroyed by heat or ethanol and has a strong inhibitory effect on thrombin. Therefore, it has a special effect on human cardiovascular and cerebrovascular diseases, especially thrombotic diseases such as cerebral thrombosis, and its prevention and treatment effect is incomparable to any other substance. Clinical studies have shown that the main pharmacological effects of Whitmania pigra polypeptide are: (1) Anticoagulant effect: A variety of substances have been found in leeches, which play inhibitory roles in different links of the coagulation mechanism, such as the thrombin inhibitor Whitmania pigra polypeptide variant family, the factor Xa inhibitor antistasin, ghilanten; the factor VIII inhibitor; the platelet glycoprotein inhibitor decorsin, ornatin; the collagen-induced platelet aggregation inhibitor Calin, Lapp, etc.; (2) Antithrombotic effect: The formation of intravascular thrombus is ultimately caused by the coagulation effect of thrombin. Leeches have a direct thrombolytic effect. It can not only bind to free thrombin in plasma, but also neutralize thrombin bound to fibrin. Therefore, Whitmania pigra polypeptide is effective against various thromboembolic diseases, especially has a significant effect on disseminated intravascular coagulation; (3) Antitumor effect: The high anticoagulant effect of leeches is conducive to the invasion of anti-cancer pharmacological active substances (such as elements like manganese, magnesium, zinc, etc.) and immune active cells into cancer tissues to kill cancer cells; (4) Hypolipidemic effect: Animal experiments have shown that leeches can significantly reduce the serum cholesterol, triglyceride, and low-density lipoprotein of hyperlipidemic animals and increase the high-density lipoprotein level; (5) Effects on cerebral hematoma and subcutaneous hematoma: Experiments have proved that thrombin is one of the neurotoxic mediators. A low dose can protect neurons and glial cells from cell death caused by hypoglycemia, ischemia, etc., while a large dose can lead to brain edema, irreversible damage to brain cells, and apoptosis of brain cells.

[0004] The polypeptide of Whitmania pigra Whitman (leech polypeptide) not only has good anticoagulant and antithrombotic effects, but also the processed and extracted polypeptide of Whitmania pigra Whitman can be used to relieve atherosclerosis and spasm, reduce the adhesiveness of blood pressure, and can significantly alleviate the symptoms of hypertension and hyperlipidemia. At the same time, relevant research also shows that the anticoagulant effect of natural polypeptide of Whitmania pigra Whitman is very specific, the anticoagulant effect is stronger than that of heparin, and the natural polypeptide of Whitmania pigra Whitman does not depend on the participation of ATⅢ and heparin cofactor Ⅱ when inhibiting thrombin activity. Compared with other anticoagulants such as heparin, the anticoagulant effect of natural polypeptide of Whitmania pigra Whitman is more reliable. Due to these above advantages, as a direct thrombin inhibitor, the natural polypeptide of Whitmania pigra Whitman has currently been recommended to replace traditional heparin and is routinely used for anticoagulant prevention and treatment of cardiovascular diseases in Europe and North America.

[0005] For example, Patent No. CN110623275A discloses a compound amino acid polypeptide food of Whitmania pigra Whitman with anticoagulant and antithrombotic effects and its preparation method. The food includes the following raw materials in parts by weight: 10-15 parts of polypeptide of Whitmania pigra Whitman; 5-8 parts of amino acids; 6-8 parts of grape seed powder; 6-8 parts of Chinese chive seed powder; 6-8 parts of Gynostemma pentaphyllum powder; 6-8 parts of black fungus powder; 5-10 parts of kudzu root powder; 5-10 parts of hawthorn powder; 5-10 parts of black sesame powder; 10-15 parts of Chinese yam powder; 5-30 parts of edible starch (added according to content requirements), but this patent does not provide corresponding experimental data to illustrate the obtained effects. Currently, in order to obtain the polypeptide of Whitmania pigra Whitman, for example, Patent No. CN102286098B discloses a production method of natural polypeptide of Whitmania pigra Whitman. The characteristics of this production method include steps such as extraction, acid precipitation, heating, dehydration and drying of the liquid containing natural polypeptide of Whitmania pigra Whitman. The obtained natural polypeptide of Whitmania pigra Whitman powder can be detected for the content of its active ingredients by the Markwardt thrombin direct titration method, so that the quality is stable and the curative effect of the product is guaranteed.

[0006] Patent No. CN104151425B, a large-scale production method of natural polypeptide of Whitmania pigra Whitman, is to first place the blood-sucking leech in vivo that has absorbed the inducing liquid in a container, add a certain amount of emetic to make it vomit the saliva in its body, namely the crude liquid of natural polypeptide of Whitmania pigra Whitman. The emetic is a zinc sulfate solution or a mixed solution of zinc sulfate and ethanol; then take out the blood-sucking leech in vivo, rinse it with clean water and continue to raise it for the next extraction; collect the crude liquid of natural polypeptide of Whitmania pigra Whitman in the container; add an appropriate amount of filler and mix, and then dry to obtain the powder finished product, which is the natural polypeptide of Whitmania pigra Whitman product. However, the above two methods do not explain the purity of the obtained polypeptide of Whitmania pigra Whitman. Summary of the Invention

[0007] The purpose of the present invention is to solve at least one of the technical problems existing in the prior art, and to provide a compound amino acid polypeptide food of Whitmania pigra Whitman and its preparation method.

[0008] To achieve the above object, the technical solution adopted by the present invention is as follows: A compound amino acid Hirudo vittata polypeptide food, wherein the polypeptide food uses Hirudo vittata polypeptide and amino acids as the main drugs as the core, and the core is externally wrapped with a first cortex, and the first cortex uses kudzu root powder, yam powder, onion powder and a lyoprotectant as the first auxiliary materials; the first cortex is externally wrapped with a second cortex, and the second cortex uses tannic acid powder, edible starch and a preservative as the second auxiliary materials, and the mass ratio of the tannic acid powder to the Hirudo vittata polypeptide is (1-5):(1-5).

[0009] Further, the tannic acid powder includes one or more of grape seed powder, hawthorn powder, apple powder, strawberry powder, and orange powder.

[0010] Further, the preservative is sorbic acid, and the mass fraction of the sorbic acid is 0.5-1%.

[0011] Further, the edible starch includes one or more of corn starch, potato starch, sweet potato starch, and cassava starch, and the mass fraction of the edible starch is 1-2%.

[0012] Further, the mass ratio of the Hirudo vittata polypeptide to the amino acids is (5-10):(1-3).

[0013] Further, the amino acids include one or more of glycine, lysine, methionine, histidine, and serine.

[0014] Further, the lyoprotectant includes one or more of trehalose, glycine, arginine, and bovine serum albumin; the mass fraction of the lyoprotectant is 0.5-1%.

[0015] A preparation method of a compound amino acid Hirudo vittata polypeptide food, for preparing the above-mentioned compound amino acid Hirudo vittata polypeptide food, the method is as follows: S1. Make the core. Pour the Hirudo vittata polypeptide and amino acids into distilled water at normal temperature and mix them to make a soft core. S2. Make the first cortex. Pour the kudzu root powder, yam powder, onion powder, and lyoprotectant into warm water at normal temperature and mix them to make a soft first cortex. Then wrap the core in the first cortex and pass through a 20-mesh sieve to obtain wet granules, and freeze-dry the wet granules. S3. Make the second cortex. Pour the tannic acid powder, edible starch and preservative into warm water at normal temperature and mix them to make a soft second cortex, and wrap the freeze-dried material in the second cortex to obtain a compound amino acid Hirudo vittata polypeptide food. Further, it also includes making the obtained compound amino acid Hirudo vittata polypeptide food into finished products in the form of tablets, granules or capsules, and then packaging them.

[0016] Further, the preparation method of the Hirudo vittata polypeptide includes the following steps: S101. Select live healthy adult blood-sucking leeches, Whitmania acranulata, purify and breed them for several days without feeding. During this period, keep clean running water. Then transfer the leeches to the extraction workshop and feed them with the induction liquid for 65 - 70 minutes until they are full, and remove the residual induction liquid and feeding equipment. The induction liquid is prepared by proportionally mixing 1 - 1.5 parts by weight of glucose powder, 0.5 - 1.0 part by weight of salt, 2 - 2.5 parts by weight of tomato juice, and 90 - 100 parts by weight of water; S102. Then inject zinc sulfate and ethanol solution as an emetic. The dosage ratio of the emetic to the live leeches full of the induction liquid is 1:5 - 6. Let it stand for 25 - 30 minutes to make the leeches full of the induction liquid vomit out the saliva in their bodies. The collected saliva is the original solution of leech polypeptide. The blood-sucking leeches, Whitmania acranulata, after extracting the original solution are re-stocked for the next extraction or sold as dried products; S103. Filter, remove impurities, and concentrate the original solution, add glacial acetic acid, stir evenly to make the original solution acidic, control the pH at 4 - 5, let it stand in an environment temperature of 20°C for 5 hours, centrifuge at low speed, remove the precipitate and take the supernatant. Then heat this supernatant to 60°C and keep the temperature unchanged for 30 minutes. After 30 minutes, let it stand and cool naturally to room temperature, centrifuge with a high-speed centrifuge, remove the residue and keep the liquid, which is the extraction solution of Whitmania acranulata polypeptide; S104. Add a freeze-drying agent and edible starch to the extraction solution of Whitmania acranulata polypeptide. The addition amount of the freeze-drying agent and edible starch is 20 - 25% of the weight of the extraction solution of Whitmania acranulata polypeptide. After fully dissolving, put it into a vacuum freeze dryer for freeze-drying, and after pulverizing, Whitmania acranulata polypeptide is obtained.

[0017] Compared with the prior art, the technical solution of this application has the following beneficial effects: 1. The polypeptide food of the present invention uses Whitmania acranulata polypeptide and amino acids as the main drugs as the core, and the core is externally wrapped with a first cortex, and the first cortex uses kudzu root powder, Chinese yam powder, and onion powder as the first auxiliary materials; the first cortex is externally wrapped with a second cortex, and the second cortex uses tannic acid powder, edible starch, and a preservative as the second auxiliary materials. The mass ratio of the tannic acid powder to the Whitmania acranulata polypeptide is (1 - 5):(1 - 5), so that the Whitmania acranulata polypeptide in this polypeptide food is not decomposed in the stomach under the protection of the tannic acid powder, and the Whitmania acranulata polypeptide exerts its medicinal effect after reaching the small intestine, improving the bioavailability.

[0018] 2. The compound amino acid Whitmania acranulata polypeptide food of the present invention has the effects of anti-blood coagulation and preventing thrombosis, reducing blood lipid and blood pressure, preventing and treating cardiovascular diseases, and enhancing immunity. It has the effects of strongly dissolving thrombus, reducing cholesterol, softening blood vessels, improving immunity, preventing blood vessel rupture, preventing and treating hypertension, hyperlipidemia, etc.

[0019] 3. The method for extracting the polypeptide of Whitmania pigra Whitman with gold border: This method uses an induction solution and an emetic to extract the saliva of the blood-sucking leech (Whitmania pigra Whitman), namely the original solution of leech polypeptide. It can avoid the problem of high mortality rate of blood-sucking leeches caused by the commonly used artificial extrusion method. The leech polypeptide extracted by the extraction method defined in the present invention has high activity, good quality, and high content of antithrombin activity. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic structural diagram of the compound amino acid Whitmania pigra Whitman polypeptide food in the preferred embodiment of the present invention; Reference signs: 1, core body; 2, first cortex; 3, second cortex. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] Next, the technical solutions in the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0022] Referring to Figure 1 As shown, in the preferred embodiment of the present invention, a compound amino acid Whitmania pigra Whitman polypeptide food, the polypeptide food takes Whitmania pigra Whitman polypeptide and amino acid as the main drugs as the core body 1, and the core body 1 is externally wrapped with a first cortex 2. The first cortex 2 uses kudzu root powder, Chinese yam powder, onion powder and a lyophilizer as the first auxiliary materials; the first cortex 2 is externally wrapped with a second cortex 3. The second cortex 3 uses tannic acid powder, edible starch and a preservative as the second auxiliary materials. The mass ratio of the tannic acid powder to the Whitmania pigra Whitman polypeptide is (1-5):(1-5), so that the Whitmania pigra Whitman polypeptide in the polypeptide food is not decomposed in the stomach under the protection of the tannic acid powder, and the Whitmania pigra Whitman polypeptide exerts its medicinal effect after reaching the small intestine, improving the bioavailability.

[0023] As a preferred embodiment of the present invention, it may also have the following additional technical features: The tannic acid powder includes one or more of grape seed powder, hawthorn powder, apple powder, strawberry powder, and orange powder. The raw materials have a wide source, low price, and are simple and easy to obtain. At the same time, the preservative, edible starch, amino acid, and lyophilizer are screened to make the prepared compound amino acid Whitmania pigra Whitman polypeptide food have uniform particles, will not crack after freeze-drying, and has high integrity, which is convenient for people to eat.

[0024] In this embodiment, the preservative is sorbic acid, and the mass fraction of the sorbic acid is 0.5-1%.

[0025] In this embodiment, the edible starch includes one or more of corn starch, potato starch, sweet potato starch, and cassava starch. The mass fraction of the edible starch is 1-2%.

[0026] In this embodiment, the mass ratio of golden-ringed leech polypeptide to amino acid is (5-10):(1-3), and the amino acid includes one or more of glycine, lysine, methionine, histidine, and serine.

[0027] In this embodiment, the lyophilizer includes one or more of trehalose, glycine, arginine, and bovine serum albumin; the mass fraction of the lyophilizer is 0.5-1%.

[0028] A preparation method of a compound amino acid golden-ringed leech polypeptide food, for preparing the above-mentioned compound amino acid golden-ringed leech polypeptide food, the method is as follows: S1. Make the core. Pour golden-ringed leech polypeptide and amino acid into distilled water at room temperature and mix to form a soft core material. S2. Make the first cortex. Pour kudzu root powder, Chinese yam powder, onion powder, and lyophilizer into warm water at room temperature and mix to form a soft first cortex material. Then wrap the core in the first cortex material, pass through a 20-mesh sieve to obtain wet granules, and freeze-dry the wet granules. S3. Make the second cortex. Pour tannic acid powder, edible starch, and preservative into warm water at room temperature and mix to form a soft second cortex material, and wrap the freeze-dried material in the second cortex material to obtain the compound amino acid golden-ringed leech polypeptide food. S4. Make the obtained compound amino acid golden-ringed leech polypeptide food into finished products in the form of tablets, granules, or capsules, and then package them.

[0029] In this embodiment, the preparation method of the golden-ringed leech polypeptide further includes the following steps: S101. Select healthy adult live blood-sucking leeches, golden-ringed leeches, purify and breed them for several days without feeding, keep clean running water during this period, then transfer the leeches to the extraction workshop, feed them with the induction liquid for 65-70 minutes until full, and remove the residual induction liquid and feeding equipment; the induction liquid is prepared by mixing 1-1.5 parts of glucose powder, 0.5-1.0 part of table salt, 2-2.5 parts of tomato juice, and 90-100 parts of water according to the following weight ratio. S102. Then inject zinc sulfate and ethanol solution as an emetic. The dosage of the emetic and the weight ratio of the live leeches that have absorbed the induction liquid is 1:5-6. Let it stand for 25-30 minutes to make the leeches that have absorbed the induction liquid vomit out the saliva in their bodies; collect the obtained saliva, which is the original leech polypeptide solution; the blood-sucking leeches, golden-ringed leeches, after extracting the original solution, are re-stocked for the next extraction or made into dry products for sale. S103. Filter, remove impurities, and concentrate the stock solution, add glacial acetic acid, stir evenly to make the stock solution acidic, control the pH at 4 - 5, let it stand for 5 hours at an ambient temperature of 20°C, centrifuge at low speed, remove the precipitate and take the supernatant, then heat this supernatant to 60°C, keep the temperature unchanged for 30 minutes, after 30 minutes, let it stand and cool naturally to room temperature, centrifuge with a high-speed centrifuge, remove the residue and keep the liquid, which is the extraction solution of leech polypeptide with gold border; S104. Add a freeze-drying agent and edible starch to the extraction solution of leech polypeptide with gold border. The addition amounts of the freeze-drying agent and edible starch are 20 - 25% of the weight of the extraction solution of leech polypeptide with gold border. After fully dissolving, place it in a vacuum freeze dryer for freeze-drying, and then obtain leech polypeptide with gold border after pulverization.

[0030] At the same time, conduct purity and activity tests on the leech polypeptide with gold border obtained by the above method and the leech polypeptide with gold border obtained by the production method of preparing natural leech polypeptide with gold border in the background technology. Through the tests, it can be known that: the purity of the leech polypeptide with gold border in this application is: 92.4%; the activity is: 5600 ATU / g; The purity of the leech polypeptide with gold border in the background technology is: 86.9%; the activity is: 1268 ATU / g.

[0031] Soluble fibrin complex (SFC) in plasma is a sensitive indicator reflecting the body's blood coagulation and fibrinolysis. Its content is closely related to the occurrence of thrombotic diseases. The SFC value of normal people is 48.5 ± 15.9 mg / L. The determination of fibrinogen degradation products (FDP) can reflect fibrinolysis activity. The increase in D-dimer in FDP is a sign of the degradation of cross-linked fibrin, and the increase in the content of fibrinopeptide A indicates the formation of thrombin, which is an early sign of the conversion of fibrinogen to fibrin. The FDP content of normal people is 23.0 ± 5.4 μg / L. The blood coagulation and fibrinolysis functions of cerebral thrombosis patients are all increased to varying degrees. The changes in thrombus can be known by measuring the FDP and SFC values of thrombus patients.

[0032] The following further illustrates the present invention through specific embodiments, but the protection scope of the present invention is not limited thereto. Embodiment

[0033] The method for preparing the compound amino acid leech polypeptide food of the present invention (the same in the following embodiments) is as follows: S1. Make the core body. Pour 85 g of leech polypeptide with gold border and 17 g of amino acid into distilled water at room temperature and mix to make a soft core body; S2. Make the first cortex. Pour 20 g of kudzu root powder, 10 g of Chinese yam powder, 5 g of onion powder, and 2 g of freeze-drying agent into warm water at room temperature and mix to make a soft first cortex. Then wrap the core body in the first cortex, pass through a 20-mesh sieve to obtain wet granules, and conduct freeze-drying on the wet granules; S3. Prepare the second cortex. Pour 85 g of tannic acid powder, 20 g of corn starch and 2 g of sorbic acid into warm water at room temperature to make the soft material of the second cortex, and wrap the freeze-dried material in the second cortex to obtain the compound amino acid golden-ringed leech polypeptide food; S4. Make the obtained compound amino acid golden-ringed leech polypeptide food into tablets (specification: 0.25 g / tablet), and then let the patient take it 3 times a day, 0.75 g each time, all orally before meals for treatment. Select 5 thrombus patients with a disease duration difference of no more than 3 months and an age of 30 - 60 years. Draw 10 mL of venous blood on an empty stomach before treatment, then centrifuge the blood at 2500 r / min for 10 min, and take the plasma to detect the SFC and FDP values of the patient without taking medicine, and record them in the following table. Take 1 month as 1 course of treatment, treat for 3 courses, and measure and record the FDP and SFC values of the thrombus patients after each course of treatment. Example

[0034] The method for preparing the compound amino acid golden-ringed leech polypeptide food of the present invention (the same in the following examples) is as follows: S1. Prepare the core. Pour 120 g of golden-ringed leech polypeptide and 36 g of amino acid into distilled water at room temperature to make the soft material of the core; S2. Prepare the first cortex. Pour 20 g of kudzu root starch, 10 g of Chinese yam starch, 5 g of onion powder and 2 g of freeze-drying agent into warm water at room temperature to make the soft material of the first cortex. Then wrap the core in the first cortex, and pass through a 20-mesh sieve to obtain wet granules, and freeze-dry the wet granules; S3. Prepare the second cortex. Pour 40 g of tannic acid powder, 20 g of corn starch and 3 g of sorbic acid into warm water at room temperature to make the soft material of the second cortex, and wrap the freeze-dried material in the second cortex to obtain the compound amino acid golden-ringed leech polypeptide food; S4. Make the obtained compound amino acid golden-ringed leech polypeptide food into tablets (specification: 0.25 g / tablet), and then let the patient take it 3 times a day, 0.75 g each time, all orally before meals for treatment. Select 5 thrombus patients with a disease duration difference of no more than 3 months and an age of 30 - 60 years. Draw 10 mL of venous blood on an empty stomach before treatment, then centrifuge the blood at 2500 r / min for 10 min, and take the plasma to detect the SFC and FDP values of the patient without taking medicine, and record them in the following table. Take 1 month as 1 course of treatment, treat for 3 courses, and measure and record the FDP and SFC values of the thrombus patients after each course of treatment. Example

[0035] The method for preparing the compound amino acid golden-ringed leech polypeptide food of the present invention (the same in the following examples) is as follows: S1. Prepare the core. Pour 90 g of golden-ringed leech polypeptide and 30 g of amino acid into distilled water at room temperature to make the soft material of the core; S2. Prepare the first cortex. Pour 20 g of kudzu root powder, 10 g of Chinese yam powder, 5 g of onion powder, and 2 g of freeze-drying agent into warm water at room temperature and mix to form a soft material for the first cortex. Then wrap the core body in the first cortex, pass through a 20-mesh sieve to obtain wet granules, and perform freeze-drying on the wet granules; S3. Prepare the second cortex. Pour 60 g of tannic acid powder, 20 g of corn starch, and 3 g of sorbic acid into warm water at room temperature and mix to form a soft material for the second cortex. Then wrap the freeze-dried material in the second cortex to obtain the compound amino acid golden-ringed leech polypeptide food; S4. Make the obtained compound amino acid golden-ringed leech polypeptide food into tablets (specification: 0.25 g / tablet), and then let the patients take it 3 times a day, 0.75 g each time, all orally before meals for treatment. Select 5 thrombus patients with a disease duration difference of no more than 3 months and an age of 30 - 60 years. Respectively, draw 10 mL of venous blood on an empty stomach before treatment, then centrifuge the blood at 2500 r / min for 10 min, take the plasma to detect the SFC and FDP values of the patient without taking medicine, and record them in the following table. Take 1 month as one course of treatment, treat for 3 courses, and measure and record the FDP and SFC values of the thrombus patients after each course of treatment. Example

[0036] The method for preparing the compound amino acid golden-ringed leech polypeptide food (the same as the following examples) of the present invention is as follows: S1. Prepare the core body. Pour 150 g of golden-ringed leech polypeptide and 30 g of amino acid into distilled water at room temperature and mix to form a soft material for the core body; S2. Prepare the first cortex. Pour 20 g of kudzu root powder, 10 g of Chinese yam powder, 5 g of onion powder, and 2 g of freeze-drying agent into warm water at room temperature and mix to form a soft material for the first cortex. Then wrap the core body in the first cortex, pass through a 20-mesh sieve to obtain wet granules, and perform freeze-drying on the wet granules; S3. Prepare the second cortex. Pour 30 g of tannic acid powder, 20 g of corn starch, and 2 g of sorbic acid into warm water at room temperature and mix to form a soft material for the second cortex. Then wrap the freeze-dried material in the second cortex to obtain the compound amino acid golden-ringed leech polypeptide food; S4. Make the obtained compound amino acid golden-ringed leech polypeptide food into tablets (specification: 0.25 g / tablet), and then let the patients take it 3 times a day, 0.75 g each time, all orally before meals for treatment. Select 5 thrombus patients with a disease duration difference of no more than 3 months and an age of 30 - 60 years. Respectively, draw 10 mL of venous blood on an empty stomach before treatment, then centrifuge the blood at 2500 r / min for 10 min, take the plasma to detect the SFC and FDP values of the patient without taking medicine, and record them in the following table. Take 1 month as one course of treatment, treat for 3 courses, and measure and record the FDP and SFC values of the thrombus patients after each course of treatment. Example

[0037] The method for preparing the compound amino acid golden-ringed leech polypeptide food (the same applies to the following examples) of the present invention is as follows: S1. Prepare the core. Pour 96 g of golden-ringed leech polypeptide and 24 g of amino acids into distilled water at room temperature and mix to form a soft core material. S2. Prepare the first cortex. Pour 20 g of kudzu root powder, 10 g of Chinese yam powder, 5 g of onion powder, and 2 g of freeze-drying agent into warm water at room temperature and mix to form a soft first cortex material. Then wrap the core in the first cortex, pass through a 20-mesh sieve to obtain wet granules, and perform freeze-drying on the wet granules. S3. Prepare the second cortex. Pour 72 g of tannic acid powder, 20 g of corn starch, and 3 g of sorbic acid into warm water at room temperature and mix to form a soft second cortex material. Wrap the freeze-dried material in the second cortex to obtain the compound amino acid golden-ringed leech polypeptide food. S4. Make the obtained compound amino acid golden-ringed leech polypeptide food into tablets (specification: 0.25 g / tablet), and then let the patients take it 3 times a day, 0.75 g each time, all orally before meals for treatment. Select 5 thrombus patients with a disease duration difference of no more than 3 months and an age range of 30 - 60 years. Draw 10 mL of venous blood on an empty stomach before treatment, then centrifuge the blood at 2500 r / min for 10 min, and take the plasma to detect the SFC and FDP values of the patient without taking medicine, and record them in the following table. Take 1 month as 1 treatment course, treat for 3 treatment courses, and measure and record the FDP and SFC values of the thrombus patients after each treatment course.

[0038] The following table shows the SFC and FDP values of each thrombus patient after 3 treatment courses: Table 1 Data of SFC and FDP values of measured thrombus patients

[0039] As shown in the table, without taking golden-ringed leech polypeptide, the FDP and SFC values of thrombus patients are much higher than the normal level. After taking the polypeptide food without this application, the FDP and SFC values of thrombus patients have decreased, but the drug effect is not very obvious. When thrombus patients take the preparations prepared by mixing tannic acid powder and golden-ringed leech polypeptide in different ratios, their FDP and SFC values both decrease significantly. And when the mass ratio of tannic acid powder to golden-ringed leech polypeptide is 2∶3 and 3∶4, the FDP and SFC values of thrombus patients decrease most significantly. And when the mass ratio of tannic acid powder to golden-ringed leech polypeptide is 3∶4, the FDP and SFC values of thrombus patients are closest to the normal level, that is, the drug effect is better.

[0040] On the premise of no conflict, those skilled in the art can freely combine and superimpose the above-mentioned additional technical features.

[0041] It is understood that the present invention is described by way of some embodiments, and those skilled in the art will be aware that, without departing from the spirit and scope of the present invention, various changes or equivalent substitutions can be made to these features and embodiments. Additionally, under the teaching of the present invention, these features and embodiments can be modified to adapt to specific circumstances and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of the present application belong to the scope protected by the present invention.

Claims

1. A compound amino acid and golden-ringed leech polypeptide food, characterized in that: The polypeptide food uses polypeptides of Whitmania pigra Whitman and amino acids as the main drugs as the core, and the core is wrapped with a first cortex. The first cortex uses kudzu root powder, Chinese yam powder, onion powder and a freeze-drying agent as the first auxiliary materials; the first cortex is wrapped with a second cortex, and the second cortex uses tannic acid powder, edible starch and a preservative as the second auxiliary materials. The mass ratio of the tannic acid powder to the polypeptides of Whitmania pigra Whitman is (1-5):(1-5).

2. The compound amino acid golden-ringed leech polypeptide food according to claim 1, characterized in that: The tannic acid powder includes one or more of grape seed powder, hawthorn powder, apple powder, strawberry powder, orange powder.

3. The compound amino acid and leech polypeptide food according to claim 1, wherein: The preservative is sorbic acid, and the mass fraction of the sorbic acid is 0.5-1%.

4. The compound amino acid and leech polypeptide food according to claim 1, wherein: The edible starch includes one or more of corn starch, potato starch, sweet potato starch, cassava starch, and the mass fraction of the edible starch is 1-2%.

5. The compound amino acid and leech polypeptide food according to claim 1, characterized in that: The mass ratio of the polypeptides of Whitmania pigra Whitman to the amino acids is (5-10):(1-3).

6. The compound amino acid and leech polypeptide food according to claim 5, characterized in that: The amino acids include one or more of glycine, lysine, methionine, histidine, serine.

7. The compound amino acid and leech polypeptide food according to claim 1, wherein: The freeze-drying agent includes one or more of trehalose, glycine, arginine, bovine serum albumin; the mass fraction of the freeze-drying agent is 0.5-1%.

8. A preparation method of a compound amino acid and golden-ringed leech polypeptide food, characterized in that: To prepare the compound amino acid and Whitmania pigra Whitman polypeptide food according to any one of claims 1-7, the method is as follows: S1. Prepare the core. Pour the polypeptides of Whitmania pigra Whitman and amino acids into distilled water at room temperature and mix to form a soft core. S2. Prepare the first cortex. Pour the kudzu root powder, Chinese yam powder, onion powder, and freeze-drying agent into warm water at room temperature and mix to form a soft first cortex. Then wrap the core in the first cortex, pass through a 20-mesh sieve to obtain wet granules, and freeze-dry the wet granules. S3. Prepare the second cortex. Pour the tannic acid powder, edible starch and preservative into warm water at room temperature and mix to form a soft second cortex, and wrap the freeze-dried material in the second cortex to obtain the compound amino acid and Whitmania pigra Whitman polypeptide food.

9. The preparation method of the compound amino acid and leech polypeptide food according to claim 8, wherein: It also includes making the obtained compound amino acid and Whitmania pigra Whitman polypeptide food into finished products in the form of tablets, granules or capsules, and then packaging.

10. The preparation method of the compound amino acid and leech polypeptide food according to claim 8, characterized in that: It also includes that the preparation method of the polypeptides of Whitmania pigra Whitman includes the following steps: S101. Select healthy adult living blood-sucking leeches Whitmania pigra Whitman, purify and breed them for several days without feeding. During this period, keep clean running water. Then transfer the leeches to the extraction workshop, feed them with an induction liquid for 65-70 minutes until full, and remove the residual induction liquid and feeding equipment; the induction liquid is prepared in proportion from 1-1.5 parts by weight of glucose powder, 0.5-1.0 part of table salt, 2-2.5 parts of tomato juice, and 90-100 parts of water. S102. Then inject a zinc sulfate and ethanol solution as an emetic. The dosage of the emetic and the weight ratio of the living leeches Whitmania pigra Whitman after sucking the induction liquid is 1:5-6. Let it stand for 25-30 minutes to make the blood-sucking leeches Whitmania pigra Whitman that have sucked the induction liquid vomit the saliva in their bodies; collect the obtained saliva, which is the original leech polypeptide solution; the blood-sucking leeches Whitmania pigra Whitman after extracting the original solution are re-stocked for the next extraction or made into dried products for sale. S103. Filter, remove impurities and concentrate the stock solution, add glacial acetic acid, stir evenly to make the stock solution acidic, control the pH at 4-5, let it stand for 5 hours at an ambient temperature of 20°C, centrifuge at low speed, remove the precipitate and take the supernatant, then heat this supernatant to 60°C, keep the temperature unchanged for 30 minutes, and after 30 minutes, let it stand and cool naturally to room temperature, centrifuge with a high-speed centrifuge, remove the residue and keep the liquid, which is the extraction solution of leech polypeptides with gold rims; S104. Add a lyophilizing agent and edible starch to the extraction solution of leech polypeptides with gold rims. The addition amounts of the lyophilizing agent and edible starch are 20-25% of the weight of the extraction solution of leech polypeptides with gold rims. After fully dissolving, place it in a vacuum freeze dryer for freeze drying, and obtain leech polypeptides with gold rims after pulverization.

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