Amphiphilic dietary fiber supramolecular aggregate as well as preparation and application thereof

Abstract

The invention discloses an amphiphilic dietary fiber supramolecular aggregate as well as preparation and application thereof, and belongs to the technical field of supramolecules and food. The supramolecular aggregate has relatively high porosity, has relatively good adsorption performance on water-soluble, fat-soluble and amphiphilic toxins, and effectively solves the technical problem of lack of gastrointestinal toxin expeller. According to the supramolecular aggregate, glucomannan, arginine, a nonionic surfactant, an alkaline solution and ethyl acetate are combined, the product is obtained through crosslinking treatment, and the method is easy to operate in the process, high in production efficiency, safe and suitable for large-scale production.

Description

Technical Field

[0001] The present invention belongs to the fields of supramolecules and food technology, and particularly relates to an amphiphilic dietary fiber supramolecular aggregate and its preparation and application. Background Art

[0002] In the early 20th century, Professor Ilya Ilyich Mechnikov, a famous immunologist in Russia and the winner of the Nobel Prize in Medicine in 1908, discovered through long-term research that human infectious diseases are not only the result of the invasion of bacteria and viruses, but more importantly, due to the toxins in the human body that damage the immune system, resulting in a decline in human immunity and causing human infection and illness. Therefore, he believed that the top priority of health is to promptly remove the toxins in the human intestinal tract, blood, lymph, skin and other systems, so as to improve the body's own immunity and the functions of various system organs, and prevent the occurrence and development of various diseases.

[0003] The sources of modern toxins include chemical toxins, cell metabolites, indigestible foods, intermediate products of body chemical reactions, and mental toxins. The sources are extensive and almost everywhere, including ceilings, floors, beds, quilts, clothes, pots, bowls, water pipes, foods, cosmetics, packaging materials, and so on.

[0004] Among them, the toxins existing in the external environment and the body include three categories: water-soluble (such as urea), fat-soluble (such as acetochlor), and amphiphilic toxins (such as endotoxin). They can be derived from the external environment or produced by the body's metabolism. 90% of the toxins entering the body come from the intestine, and 80% of the toxins in the body are excreted from the intestine to the outside of the body. Therefore, the research and development of gastrointestinal toxin excretors is of the most important significance for human health.

[0005] At present, the commonly used product is mainly activated carbon. However, activated carbon has a certain adsorption effect on fat-soluble toxins, but has a poor adsorption effect on water-soluble and amphiphilic toxins. Summary of the Invention

[0006] Aiming at the lack of gastrointestinal toxin excretors at present, the first object of the present invention is to provide an amphiphilic dietary fiber supramolecular aggregate, which has a high porosity and has good adsorption properties for water-soluble, fat-soluble, and amphiphilic toxins, effectively solving the technical problem of the lack of gastrointestinal toxin excretors.

[0007] The second object of the present invention is to provide a preparation method of an amphiphilic dietary fiber supramolecular aggregate. This preparation method obtains the product through cross-linking treatment. The process of this method is easy to operate, has high production efficiency, is safe, and is suitable for large-scale production.

[0008] The second object of the present invention is to provide an application of an amphiphilic dietary fiber supramolecular aggregate, which can be used as a gastrointestinal toxin excretion agent and is widely used in the fields of food and medicine.

[0009] The present invention is achieved through the following technical solutions:

[0010] An amphiphilic dietary fiber supramolecular aggregate, the supramolecular aggregate comprising the following components: a non-ionic surfactant and glucomannan;

[0011] The non-ionic surfactant is a molecule that does not dissociate in an aqueous solution, does not contain charged ionic groups in its molecular structure, and has a dispersing and emulsifying effect on water and oil;

[0012] The non-ionic surfactant includes one or a combination of several of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, polyol polyoxyethylene ether fatty acid ester, fatty acid methyl ester ethoxylate, alkyl alcohol amide, polyol mono-fatty acid ester, sorbitan ester, sucrose ester, alkylamine oxide, N-alkyl pyrrolidone, and lecithin.

[0013] Preferably, the fatty alcohol polyoxyethylene ether is cetearyl alcohol polyether-10 or cetearyl alcohol polyoxyethylene ether-10;

[0014] The alkylphenol polyoxyethylene ether is nonylphenol polyether-10 or nonylphenol polyoxyethylene ether;

[0015] The polyol polyoxyethylene ether fatty acid ester is polysorbate-20;

[0016] The alkyl alcohol amide is coconut oil amide DEA;

[0017] The polyol mono-fatty acid ester is any one of glyceryl stearate, glycerol monooleate, and diglyceride;

[0018] The sorbitan ester is sorbitan laurate;

[0019] The alkylamine oxide is lauryl amine oxide;

[0020] The N-alkyl pyrrolidone is lauryl pyrrolidone;

[0021] The lecithin is soy lecithin or egg yolk lecithin.

[0022] A preparation method of an amphiphilic dietary fiber supramolecular aggregate, comprising the following steps:

[0023] Glucomannan, arginine, non-ionic surfactant and water are selected for homogeneous dispersion pretreatment followed by heat preservation treatment. Then, an alkaline solution and ethyl acetate are added, and after high-temperature cross-linking for multi-phase pore formation, low-temperature freezing for shaping, dissolution and separation of pore-forming molecules, centrifugal elution, and drying and pulverization, the process is completed.

[0024] To solve the above technical problems, the supramolecular aggregate uses a non-ionic surfactant with good detergency as a toxin carrier. However, when non-ionic surfactant molecules enter the intestine, while they can carry toxins, they can also be absorbed, endangering human health. To avoid the absorption of non-ionic surfactants, the supramolecular aggregate is constructed into an insoluble supramolecular material, with the non-ionic surfactant as one of the components of the supramolecular material.

[0025] To increase the toxin-carrying efficiency of non-ionic surfactants, an ideal supramolecular material should have a certain porosity. The supramolecular aggregate selects glucomannan as the building unit. Glucomannan has a long molecular chain, and the molecular chains are prone to entanglement and assembly into supramolecules. The long chain contains branches and acetyl groups, which can prevent the entanglement of molecular chain segments and form pores.

[0026] At the same time, arginine is selected as a processing aid. Firstly, it protects the acetyl group by forming an amide bond. Secondly, arginine adsorbs water molecules, reduces the water molecules between entangled molecules, and promotes supramolecular assembly. Thirdly, arginine molecules dissolve in water and also act as solid-phase pore-forming molecules.

[0027] The supramolecular aggregate selects non-ionic surfactant molecules as toxin-carrying molecules. Firstly, non-ionic surfactants have high safety. Secondly, they are uncharged and will not have a serious inhibitory effect on the entanglement and assembly of glucomannan molecules. Thirdly, glucomannan molecules are also neutral molecules. When non-ionic surfactant molecules approach glucomannan molecules, intermolecular long-range gravitational potential energy is easily generated, promoting supramolecular assembly.

[0028] To promote the supramolecular assembly of glucomannan molecules and non-ionic surfactants, the supramolecular aggregate's supramolecular assembly process optimizes the quantity ratio and concentration of glucomannan molecules, non-ionic surfactant molecules, and arginine molecules. A suitable concentration keeps the intermolecular distance in a suitable mesoscopic state for entanglement. Molecular homogeneous dispersion pretreatment and supramolecular assembly process separation are adopted. After mixing the three molecules, high-temperature treatment is carried out to promote the uniform interpenetration of molecules, and an alkaline solution is added to promote intermolecular entanglement and assembly.

[0029] To increase the porosity and promote supramolecular assembly, carbonate bases (sodium bicarbonate, sodium carbonate) and ethyl acetate are selected for the supramolecular aggregate. The base promotes molecular entanglement. Ethyl acetate decomposes into acetic acid and ethanol at high temperature. Acetic acid reacts with the carbonate base to produce CO2. CO2 and ethanol perturb between molecules, promoting intermolecular entanglement. At the same time, pores and pore channels are formed to achieve the purpose of gas-phase pore formation. The unreacted base and ethyl acetate also serve the purpose of solid-phase pore formation.

[0030] Preferably, based on a total of 100 parts, the mass parts ratio of each raw material is 3-5 parts of glucomannan, 0.5-1.5 parts of non-ionic surfactant, 0.1-0.5 parts of arginine, 0.2-1.0 parts of ethyl acetate, 10 parts of an alkaline solution with a mass concentration of 1wt%-5wt%, and the balance is water.

[0031] Preferably, the alkaline solution is a sodium carbonate solution or a sodium bicarbonate solution.

[0032] Preferably, when performing the heat preservation treatment after the homogeneous dispersion pretreatment, it is to keep warm at 90-120°C for 60min-90min.

[0033] Preferably, at a high temperature of 90-120°C, a cross-linking multi-phase pore formation treatment is carried out by keeping warm for 60-180min.

[0034] Preferably, at a low temperature of -80°C to -20°C, a shaping treatment is carried out by freezing for 2-4h.

[0035] In the preparation method of the supramolecular aggregate, a freeze-thaw shaping process is adopted to further increase the stability of the supramolecular material, and the surfactant molecules and glucomannan molecules that are not firmly assembled are removed by rinsing and centrifugation. In this preparation method, common raw materials are used, the production preparation method is easy to operate, and the production efficiency is high, which is suitable for large-scale production.

[0036] Application of an amphiphilic dietary fiber supramolecular aggregate in the preparation of gastrointestinal detoxifying agents.

[0037] Compared with the prior art, the present invention has at least the following technical effects:

[0038] The present invention provides an amphiphilic dietary fiber supramolecular aggregate, which has a relatively high porosity and has good adsorption performance for water-soluble, fat-soluble, and amphiphilic toxins, effectively solving the technical problem of the lack of gastrointestinal toxin excretion agents.

[0039] Among them, (1) The supramolecular aggregate preferably selects the ratio and concentration of non-ionic surfactant, glucomannan, and arginine, and carefully selects the processing aids carbonate base and ethyl acetate. It separates the molecular homogeneous dispersion pretreatment from the supramolecular assembly process, and creatively assembles non-ionic surfactant molecules and glucomannan molecules into supramolecules. While fully exerting the toxin-carrying ability of non-ionic surfactant molecules, it confines the action of non-ionic surfactants within the gastrointestinal lumen, and the molecules are not absorbed into the blood, achieving the purpose of excreting gastrointestinal toxins from the product, being safe and effective;

[0040] (2) The supramolecular aggregate cleverly applies the principles of tissue engineering, makes full use of the molecular characteristics of the branched chains and acetyl groups on the glucomannan molecules for site-directed pore formation and gas-phase pore formation, and successfully constructs a supramolecular material with rich pores;

[0041] (3) In this supramolecular aggregate, arginine, carbonate base, and ethyl acetate are carefully selected as processing aids. Arginine protects the acetyl group, and arginine and carbonate base jointly promote intermolecular interactions. Ethyl acetate produces acetic acid and ethanol at high temperatures. Acetic acid reacts with carbonate base to produce CO2, and ethanol and CO2 play a role in gas-phase pore formation, while the residual processing aids play a role in solid-phase pore formation.

[0042] (4) The raw materials used in this supramolecular aggregate are all of food or pharmaceutical grade, with simple types, no toxic substances are introduced, the preparation process of the supramolecular material is simple, easy to scale up industrially, avoids the generation of harmful substances during the production process, and is easy to operate, enabling large-scale production.

[0043] (5) This supramolecular aggregate product can be used as a detoxifying agent for the gastrointestinal tract and can be widely applied in the food and pharmaceutical fields. Description of the Drawings

[0044] Figure 1 Schematic diagram of the sample after centrifugal elution in Example 1;

[0045] Figure 2 Schematic diagram of the sample after drying in Example 1. Detailed Embodiments

[0046] The following will describe the implementation schemes of the present invention in detail in conjunction with the examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. For the specific conditions not specified in the examples, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer. For the reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0047] Example 1:

[0048] A preparation method of an amphiphilic dietary fiber supramolecular aggregate includes the following steps:

[0049] 4 kg of glucomannan, 0.8 kg of soy lecithin, 0.1 kg of arginine, and 95.1 kg of water were subjected to homogeneous dispersion pretreatment, kept at 100 °C for 80 min, 10 kg of 3.5% sodium bicarbonate solution and 0.5 kg of ethyl acetate were added, stirred and mixed evenly, kept at 100 °C for 150 min, cryogenically frozen at -45 °C for 10 h for shaping, crushed and rinsed, centrifugally eluted, dried and pulverized to obtain an amphiphilic dietary fiber supramolecular aggregate.

[0050] As Figure 1 shown, it is a schematic diagram of the sample after centrifugal elution;

[0051] As Figure 2 shown, it is a schematic diagram of the sample after drying.

[0052] Example 2:

[0053] A method for preparing an amphiphilic dietary fiber supramolecular aggregate includes the following steps:

[0054] 5 kg of glucomannan, 1.0 kg of diglyceride, 0.5 kg of sucrose ester, 0.5 kg of arginine, and 93.0 kg of water were subjected to homogeneous dispersion pretreatment, kept at 120 °C for 90 min, 10 kg of 2% sodium bicarbonate + 3% sodium carbonate solution and 0.1 kg of ethyl acetate were added, stirred and mixed evenly, kept at 120 °C for 180 min, cryogenically frozen at -20 °C for 24 h for shaping, crushed and rinsed, centrifugally eluted, dried and pulverized to obtain an amphiphilic dietary fiber supramolecular aggregate.

[0055] Example 3:

[0056] A method for preparing an amphiphilic dietary fiber supramolecular aggregate is specifically as follows:

[0057] 3 kg of glucomannan, 0.5 kg of cetostearyl polyoxyethylene ether - 10, 0.3 kg of arginine, and 96.2 kg of water were subjected to homogeneous dispersion pretreatment, kept at 90 °C for 60 min, 10 kg of 1% sodium carbonate solution and 1.0 kg of ethyl acetate were added, stirred and mixed evenly, kept at 90 °C for 90 min, cryogenically frozen at -80 °C for 2 h for shaping, crushed and rinsed, centrifugally eluted, dried and pulverized to obtain an amphiphilic dietary fiber supramolecular aggregate.

[0058] Setting of comparative examples:

[0059] For further illustration and comparison, by changing the component or ratio or process flow parameters of the present invention, the products prepared all have defects, as shown in the following table:

[0060]

[0061]

[0062] The above comparison results, such as the result data of porosity or adsorption performance, are as follows.

[0063] Test example: The amphiphilic dietary fiber supramolecular aggregates prepared in each example were taken as test samples for relevant performance determination.

[0064] Determination method:

[0065] 1. The porosity of the material was determined by mercury intrusion method.

[0066] 1.1 Determination of water-holding capacity and oil-holding capacity: 0.2 g of the test samples of each example or granular activated carbon were weighed and placed in 20 mL of edible lard, peanut oil, and distilled water respectively, shaken in a shaker at 180 r / min, 37 °C in the dark for 4 h, centrifuged at 4000 r / min for 20 min, the supernatant was discarded, and the residue was blotted dry with filter paper to remove free oil or water, weighed, and the water-holding and oil-holding amounts were calculated.

[0067] 1.2 Determination of static adsorption capacity and adsorption rate of urea, endotoxin, acetochlor, etc.: 30 mL (V0) of phosphate buffer test solution (pH 7.0) was prepared with appropriate amounts of urea, endotoxin, and acetochlor respectively. The test solution was added to a 50 mL centrifuge tube, and 0.3 g of the test samples of each example or granular activated carbon was added. It was shaken in a shaker at 180 r / min, 37 °C in the dark for 4 h, filtered through a 0.22 μm needle filter, the filtrate volume (V1) and absorbance value were measured, the concentration of the test sample was converted according to the standard curve, and the adsorption amount and adsorption rate (%) were calculated according to the formula:

[0068] Adsorption amount = C0V0 - λC1V1 / M;

[0069] Adsorption rate = (C0V0 - λC1V1) / C0V0 × 100%

[0070] Among them, C0 and C1 are the initial concentration of the analyte in the test solution and the concentration in the filtrate respectively, λ is the correction coefficient after adding the supramolecular material, λ = 1; V0 and V1 are the initial volume of the test solution to be adsorbed and the volume of the filtrate.

[0071] 1.3 Effects of the test samples of each example on blood lipid, blood glucose, blood endotoxin, blood ammonia, blood glucagon, and fecal properties of cirrhotic rats: 12 male Wistar rats were taken to establish a cirrhotic model.

[0072] The test sample groups of each example consisted of 12 animals. Each test sample of each example was administered by gavage at a dose of 0.1 g / 100 g per day, and the rest was the same as the liver cirrhosis model group. All animals were injected at the same time and fasted overnight before injection. On the first day of the experiment, feces of each group of rats were collected 6 hours after gavage, and the number of fecal pellets of the rats was calculated. After continuously collecting for 10 days, the total weight of the feces was weighed and dried at 100 °C for 30 minutes. The fecal water content was calculated according to the following formula:

[0073] Fecal water content (%) = (fecal fresh weight - fecal dry weight (g)) / fecal fresh weight (g) × 100%. At 4 hours after intraperitoneal injection of Escherichia coli endotoxin in the 9th week, ether anesthesia was performed and blood was collected from the abdominal aorta. Plasma blood lipids, blood glucose, endotoxin, blood ammonia, and blood insulin levels were measured using an automatic blood biochemical analyzer.

[0074] 1.4 Determination of the static adsorption capacity and adsorption rate for blood glucose, triglyceride, total cholesterol, endotoxin, and blood ammonia: Rats in the liver cirrhosis model group were decapitated to collect blood, centrifuged at 3500 r / min for 15 minutes, and 2 mL of plasma was taken. Either 0.02 g of supramolecular material or granular activated carbon was added or not added, and the mixture was shaken at 180 r / min in a light-protected shaker at 37 °C for 4 hours, then centrifuged at 4000 r / min for 20 minutes. The static adsorption capacity and adsorption rate for blood glucose, triglyceride, total cholesterol, endotoxin, and blood ammonia were measured, with λ = 1.

[0075] Statistical data were analyzed by variance using SPSS 11.0 software. Each group of data was expressed as x ± s, and P < 0.05 was considered statistically significant.

[0076] 1.5 Research results

[0077] 1.5.1 The porosities of the test samples of each example and granular activated carbon are shown in Table 1 below.

[0078] Table 1 Porosities of supramolecular materials and granular activated carbon (%)

[0079] Product Porosity (%) Product Porosity (%) Granular activated carbon 51.23％ Example 1 52.08％ Example 2 49.8％ Example 3 51.01％ Comparative Example 3 12.71％ Comparative Example 5 11.09％ Comparative Example 6 10.09％ Comparative Example 8 47.09％ Comparative Example 12 56.25％

[0080] As can be seen from Table 1, the test samples of Examples 1-3 and Comparative Examples 8 and 12 had porosities comparable to those of activated carbon particles.

[0081] 1.5.2 Adsorption amounts of the test samples of each example for water and oil

[0082] Table 2 Adsorption amounts of the test samples of each example for water and oil (g / g)

[0083] Product Lard Peanut oil Distilled water Example 1 <![CDATA[9.74±0.36 # > <![CDATA[9.16±0.91 # > <![CDATA[8.13±1.07 #* > Example 2 <![CDATA[8.23±0.29 # > <![CDATA[8.47±0.46 # > <![CDATA[8.95±1.00 #* > Example 3 <![CDATA[9.01±0.41 # > <![CDATA[8.72±0.89 # > <![CDATA[8.55±0.92 #* > Comparative Example 8 2.11±0.18 2.12±0.37 9.03±1.23 Comparative Example 12 2.45±0.22 2.05±0.98 6.33±0.62 Granular activated carbon 0.49±0.25 0.55±0.33 2.81±0.19

[0084] Note: # Compared with the adsorption capacity of granular activated carbon, P < 0.01.

[0085] As can be seen from Table 2, compared with granular activated carbon, Examples 1-3 have good adsorption properties for both water and oil and are amphiphilic. The comparative example has a lower adsorption capacity for oil and insufficient lipophilicity. Example 1 was selected for further efficacy verification.

[0086] 1.5.3 Static adsorption capacity of urea, endotoxin, and acetochlor:

[0087] Table 3 Adsorption capacity and adsorption rate of urea, endotoxin, and acetochlor

[0088]

[0089]

[0090] Note: Compared with the adsorption capacity of granular activated carbon, P < 0.01.

[0091] As can be seen from Table 3, the adsorption capacity of Example 1 for urea, endotoxin, and acetochlor is significantly higher than that of granular activated carbon (P < 0.01).

[0092] 1.5.4 Effects of Example 1 on blood lipid, blood glucose, blood endotoxin, blood ammonia, blood insulin levels, and fecal properties in cirrhotic rats:

[0093] Table 4 Effects on blood lipid, blood glucose, blood endotoxin, blood ammonia, and blood insulin in cirrhotic rats

[0094]

[0095] Note: # Compared with the normal group, P < 0.05; * compared with the cirrhotic model group, P < 0.05.

[0096] As can be seen from Table 4, Example 1 can significantly reduce the levels of blood triglyceride, total cholesterol, low-density lipoprotein cholesterol, blood glucose, blood endotoxin, blood ammonia, and blood insulin in cirrhotic rats, and can increase the content of high-density lipoprotein cholesterol (P < 0.05).

[0097] Table 5 Effects of Example 1 on fecal properties in cirrhotic rats

[0098] Group Number of fecal pellets (pcs) Fecal weight (g) Fecal water content (%) Normal group 121.52±8.90 1.25±0.06 56.99±6.14 Liver cirrhosis model group <![CDATA[61.12±7.17 # > <![CDATA[0.53±0.06 # > <![CDATA[24.19±4.04 # > Group gavaged with materials of Example 1 <![CDATA[147.28±10.12 * > <![CDATA[3.07±0.46 * > <![CDATA[69.95±8.07 * >

[0099] Note: # Compared with the normal group, P < 0.05; * compared with the cirrhotic model group and the normal group, P < 0.05.

[0100] As can be seen from Table 5, the number of feces in the cirrhotic model group rats decreased, and the water content of feces decreased. After intragastric administration of Example 1, the number, weight, and water content of feces exceeded those of the normal group. The dry weight and water content of feces in Example 1 were approximately 6 times and 3 times those of the cirrhotic group.

[0101] 1.5.5 Static adsorption capacity and adsorption rate of Example 1 for triglyceride, total cholesterol, blood glucose, blood endotoxin, and blood ammonia:

[0102] Table 6 Adsorption capacity and adsorption rate for blood triglyceride, total cholesterol, blood endotoxin, and blood ammonia

[0103]

[0104] Note: # By analysis of variance, compared with the adsorption capacity of granular activated carbon, the adsorption capacity of Example 1 has statistical difference, P < 0.01.

[0105] As can be seen from Table 6, the adsorption performance of Example 1 for blood triglyceride, total cholesterol, blood ammonia, and blood endotoxin is significantly higher than that of granular activated carbon (P < 0.01).

[0106] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An amphiphilic dietary fiber supramolecular aggregate, characterized in that, The supramolecular aggregate comprises the following components: a non-ionic surfactant and glucomannan; The non-ionic surfactant is a molecule that does not dissociate in an aqueous solution, does not contain charged ionic groups in its molecular structure, and has a dispersing and emulsifying effect on water and oil; The non-ionic surfactant includes one or a combination of several of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, polyol polyoxyethylene ether fatty acid ester, fatty acid methyl ester ethoxylate, alkyl alcohol amide, polyol mono-fatty acid ester, sorbitan ester, sucrose ester, alkyl amine oxide, N-alkyl pyrrolidone, and lecithin.

2. The amphiphilic dietary fiber supramolecular aggregate according to claim 1, characterized in that, The fatty alcohol polyoxyethylene ether is cetearyl alcohol polyether-10 or cetearyl alcohol polyoxyethylene ether-10; The alkylphenol polyoxyethylene ether is nonylphenol polyether-10 or nonylphenol polyoxyethylene ether; The polyol polyoxyethylene ether fatty acid ester is polysorbate-20; The alkyl alcohol amide is coconut diethanolamide; The polyol mono-fatty acid ester is any one of glyceryl stearate, glycerol monooleate, and diglyceride; The sorbitan ester is sorbitan laurate; The alkyl amine oxide is lauryl amine oxide; The N-alkyl pyrrolidone is lauryl pyrrolidone; The lecithin is soy lecithin or egg yolk lecithin.

3. A preparation method of the amphiphilic dietary fiber supramolecular aggregate as described in claim 1, characterized in that, It includes the following steps: Select glucomannan, arginine, non-ionic surfactant and water, carry out homogeneous dispersion pretreatment and then heat preservation treatment, then add an alkaline solution and ethyl acetate, and after high-temperature cross-linking multi-phase pore formation, low-temperature freezing shaping, dissolution and separation of pore-forming molecules, centrifugal elution, drying and pulverization, it is completed.

4. The preparation method of an amphiphilic dietary fiber supramolecular aggregate according to claim 3, wherein, Based on a total of 100 parts, the mass ratio of each raw material is 3-5 parts of glucomannan, 0.5-1.5 parts of non-ionic surfactant, 0.1-0.5 parts of arginine, 0.2-1.0 parts of ethyl acetate, 10 parts of an alkaline solution with a mass concentration of 1wt%-5wt%, and the balance is water.

5. The preparation method of an amphiphilic dietary fiber supramolecular aggregate according to claim 4, characterized in that, The alkaline solution is a sodium carbonate solution or a sodium bicarbonate solution.

6. The preparation method of an amphiphilic dietary fiber supramolecular aggregate according to claim 3, characterized in that, When carrying out heat preservation treatment after homogeneous dispersion pretreatment, it is to keep warm at 90-120°C for 60min-90min.

7. The preparation method of an amphiphilic dietary fiber supramolecular aggregate according to claim 3, characterized in that, At a high temperature of 90-120°C, carry out cross-linking multi-phase pore formation treatment by heat preservation treatment for 60-180min.

8. The preparation method of an amphiphilic dietary fiber supramolecular aggregate according to claim 3, characterized in that, At a low temperature of -80°C to -20°C, carry out shaping treatment by freezing for 2-4h.

9. Use of an amphiphilic dietary fiber supramolecular aggregate as described in claim 1 in the preparation of a gastrointestinal detoxifying agent.

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