A body fluid sample collection device for pleural effusion and method of use thereof

Abstract

The present application belongs to the technical field of surgical instruments, and provides a body fluid sample collection device for pleural and peritoneal effusion and a use method thereof. The present application is provided with a pretreatment device between a body fluid bottle and a suction pipeline, the pretreatment device comprising a viscosity control part, the viscosity control part being provided with a tapered tube, the inner diameter of the tapered tube near the end of the suction pipeline being smaller than the inner diameter of the end away from the suction pipeline, both ends of the tapered tube being provided with openable and closable branch pipe openings, the branch pipe openings comprising a first branch pipe opening and a second branch pipe opening, the first branch pipe opening being closer to the suction pipeline than the second branch pipe opening. The present application avoids the blockage of the suction pipeline and the influence on the accuracy of microscopic examination caused by the fibrin clots of high-viscosity pleural and peritoneal effusion during the suction process; avoids the need for multiple suction of pleural and peritoneal effusion and the blockage of the pipeline caused by residual clots in the pipeline before and after the suction process of the indwelling suction of pleural and peritoneal effusion, and even the need for replacement of the indwelling and suction instruments.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of surgical instruments, and provides a body fluid sample collection device for pleural effusion and ascites and a method for using the same. BACKGROUND

[0002] Pleural effusion and ascites are common pathological signs in clinical practice, which are often caused by malignant tumors, cirrhosis, heart failure, infectious diseases or hypoproteinemia, etc. Obtaining pleural effusion and ascites samples through puncture for biochemical detection, cytological analysis and microbial culture has important value for identifying the cause, guiding treatment and prognosis evaluation. At present, the clinical collection methods mainly include traditional puncture needle combined with syringe aspiration or connection with drainage bag, but there are the following technical defects: sample pollution risk, open operation is easy to cause air contact or instrument pollution, especially when cytological examination is performed, the mixing of external microorganisms or impurities may cause false positive misjudgment. Studies show that according to the data of Clinical Laboratory Journal in 2022, the microbial contamination rate of samples collected by open method can reach 12% to 15%; effective component degradation, the traditional device lacks anticoagulation protection mechanism, and fibrin coagulation can cause cell clumps to form, affecting the accuracy of microscopic examination; low operation efficiency, manual multiple aspiration needs to repeatedly disassemble the instrument, increasing the risk of pneumothorax or organ injury. The prior art CN112972783B discloses a device for separating and purifying tumor cells in malignant pleural effusion and ascites, which comprises a suction pipeline, a centrifugal device and a centrifugal bottle assembly. The centrifugal device is composed of a fixing seat, a control panel, a servo motor, a coupling, a clamping sleeve, a placing groove, a sliding groove, a spring, a clamping seat and an avoidance hole. The centrifugal bottle assembly is composed of a lower bottle body, a lower centrifugal drum, a filter layer, an upper bottle body, an upper centrifugal drum and a rubber plug. The inventor believes that the prior art has room for improvement. SUMMARY

[0003] The present application aims to avoid the blockage of the suction pipeline and the influence on the accuracy of microscopic examination caused by the fibrin clots of high-viscosity pleural effusion and ascites during the suction process. Secondly, it avoids the need for multiple suction of pleural effusion and ascites and the residual clots in the pipeline before and after the suction process of the indwelling suction of pleural effusion and ascites, which may cause pipeline blockage and even require replacement of indwelling and suction instruments; preliminary separation of cells and liquid in the pleural effusion and ascites sample facilitates subsequent biochemical detection and cytological analysis.

[0004] The application discloses a body fluid sample collection device for ascites, which comprises a suction pipeline and a body fluid bottle connected with the suction pipeline, and a pretreatment device is arranged between the body fluid bottle and the suction pipeline. The pretreatment device comprises a viscosity control part, and the viscosity control part is provided with a tapered tube. The inner diameter of the tapered tube at the end close to the suction pipeline is smaller than the inner diameter of the end away from the suction pipeline. Both ends of the tapered tube are provided with openable and closable branch pipe openings. The branch pipe openings comprise a first branch pipe opening and a second branch pipe opening. The first branch pipe opening is closer to the suction pipeline than the second branch pipe opening. During the suction process, the high-viscosity ascites sample passes through the tapered tube with gradually increased inner diameter. The increase of the cross-sectional area through which the ascites sample passes can effectively avoid the blockage of the ascites sample in the pipeline. The two ends of the tapered tube are provided with openable and closable branch pipe openings. The anticoagulant can be added through the first branch pipe opening according to the viscosity of the ascites sample. The anticoagulant and the ascites sample are fully mixed in the tapered tube, the ascites sample in the tapered tube is diluted, and the anticoagulant is added through the second branch pipe opening before and / or after use. The anticoagulant fully washes or seals the tapered tube and the pipeline connected with the suction pipeline, so that the residual ascites sample in the pipeline is avoided to cause the blockage of the pipeline. The anticoagulant can be normal saline, heparin saline and the like.

[0005] As preferred, the viscosity control part is provided with at least two electrodes, and a pressure difference exists between the electrodes. The inner wall of the tapered tube is provided with a conductive coating. The conductive coating of the inner wall of the tapered tube and the electrodes of the viscosity control part are used to apply a direct current field to the tapered tube to form an electroosmotic flow. The conductive coating can be a nano-porous silicon oxide coating, and the electrodes can be titanium alloy plated with platinum. A 1-5 V / mm electric field can improve the flow rate of the ascites simulation solution with a viscosity of 1.5-5 cP by 30%-50% under other same conditions. At the same time, the electric field force directly acts on the liquid molecules in the ascites simulation solution, so that the deposition of cell fragments or fibrin in the pipe wall and the blockage in the flow process can be inhibited or even separated.

[0006] As preferred, the end of the viscosity control part close to the suction pipeline is provided with a first sealing section, the first sealing section connects the tapered tube and the suction pipeline, the inside of the first sealing section is provided with a first electrode, the end of the viscosity control part away from the suction pipeline is provided with a second sealing section, the second sealing section connects the viscosity control part and a centrifugal part, the second sealing section is provided with a second electrode, and a pressure difference exists between the first electrode and the second electrode. The above arrangement is used to control the action time and range of the electroosmotic flow, so as to avoid the change of the biochemical characteristics of the ascites caused by the electroosmotic flow. At the same time, the electrodes and the patient are separated by the suction pipeline and the pipeline interval, so that the tissue damage of the patient caused by the voltage can be avoided.

[0007] As preferred, the pretreatment device further comprises a centrifugal part, the centrifugal part comprises a first shell, an inner circle of the first shell is provided with a sample inlet end connected with the second sealing section. The centrifugal part is arranged to screen the mixture in the drained pleural effusion sample, so as to facilitate different biochemical detection and cytological analysis of different components of the drained pleural effusion sample.

[0008] As preferred, an outer circle of the first shell is provided with a clear liquid outlet end connected with the body fluid bottle, the sample inlet end is located below the clear liquid outlet end, and a cell screen is arranged between the sample inlet end and the clear liquid outlet end. During centrifugation, the first shell rotates, and the clear liquid and part of the small-particle-size cell debris and other particles are screened to the upper layer of the sample, the clear liquid and the small-particle-size particles are collected by the body fluid bottle, the precipitate and the large-particle-size cell debris and other particles sink to the bottom of the sample, and the separation of the pleural effusion sample is completed. With the above arrangement, the cell screen can prevent part of the precipitate and large-particle-size debris from being screened out of the clear liquid outlet end during the early centrifugation process, and can improve the separation rate of the sample. In addition, the pleural effusion can flush the surface of the cell screen during centrifugal screening, so as to avoid the blockage of the cell screen and the frequent cleaning of the cell screen.

[0009] As preferred, the second sealing section is provided with a one-way valve, and the one-way valve is used to make the internal medium of the pretreatment device flow from the viscosity control part to the centrifugal part. Before and / or after use of the body fluid sample collection device and / or when the pipeline is blocked, anticoagulant needs to be injected into the second branch pipe opening. The second sealing section provided with the one-way valve can prevent the anticoagulant for flushing the pipeline and sealing the pipeline from flowing backward, causing pipeline pollution, and can prevent the centrifugally screened pleural effusion sample from flowing backward, causing the repeatedly screened sample to be repeatedly screened, and can prevent the prolongation of the pleural effusion drainage time.

[0010] As preferred, the centrifugal part further comprises a second shell, and a centrifugal power device is fixed in the second shell. An output end of the centrifugal power device is connected with the bottom of the first shell.

[0011] As preferred, the first shell is provided with a precipitate outlet end, the top of the second shell is provided with a precipitate inlet end, the precipitate inlet end is in communication with the precipitate outlet end, and the precipitate outlet end is located at the lower part of the side wall of the first shell. The second shell is arranged to collect the precipitate and large-particle-size particles which have been centrifugally screened in time, the body fluid bottle is arranged to collect the clear liquid and small-particle-size particles which have been centrifugally screened in time, the accumulation of the pleural effusion sample in the first shell is avoided, the repeated screening of the sample is avoided, the volume of the first shell is reduced, the required power of the centrifugal power device of the first shell is reduced, the small-sized centrifugal power device is facilitated to be selected, and the total weight of the body fluid sample collection device is reduced.

[0012] Preferably, the second shell comprises at least two parts, including a collection part and a cover part on top of the collection part, the cover part is provided with a sediment inlet end, and the cover part separates the inside of the collection part from the external environment. With the above arrangement, the collection part of the second shell can be disassembled and replaced, which facilitates timely delivery of the collected sediment and large-particle part for inspection, and at the same time does not affect the continuous processing and screening of the pleural effusion sample by the body fluid sample collection device; the collection part is similar in shape to a culture dish, which facilitates subsequent direct delivery of the collection part for inspection, reduces the risk of sample exposure and contamination during sample transfer, and the cover part keeps the inside of the collection part isolated from the external environment, thereby avoiding sample contamination during collection of the pleural effusion sample.

[0013] A method for using a body fluid sample collection device for pleural effusion, which is applicable to the body fluid sample collection device for pleural effusion described above. The body fluid sample collection device can add anticoagulant to the viscosity control part through the second branch pipe opening before and / or after use and / or when the pipeline is blocked. During use, the body fluid sample collection device can add anticoagulant to the viscosity control part through the first branch pipe opening. During the suction process, high-viscosity pleural effusion samples pass through the tapered pipe with gradually increasing inner diameter, and the increase in the cross-sectional area through which the pleural effusion sample passes can effectively prevent the pleural effusion sample from being blocked by clots in the pipeline; the two ends of the tapered pipe are provided with openable branch pipe openings, anticoagulant can be added through the first branch pipe opening according to the viscosity of the pleural effusion sample, the anticoagulant and the pleural effusion sample are fully mixed in the tapered pipe, the pleural effusion sample in the tapered pipe is diluted, and anticoagulant is added through the second branch pipe opening before and / or after use, which fully cleans or seals the pipeline connecting the suction pipeline, thereby preventing residual pleural effusion samples from causing pipeline blockage.

[0014] The present application solves the problems of blockage of the suction pipeline and influence on microscopic examination accuracy caused by fibrin clots and the like of high-viscosity pleural effusion during pleural effusion suction, blockage of the pipeline caused by residual clots in the pipeline before and after multiple suction of pleural effusion and indwelling and suction of pleural effusion, and the like, and has the following beneficial effects: the flow rate of the pleural effusion simulation solution is increased by 30% to 50% under other equivalent conditions; the liquid molecules in the pleural effusion simulation solution are directly acted on by the electric field force, which further inhibits or separates the deposition of cell fragments or fibrin in the pipe wall and the clots in the flow process; the small-sized centrifugal power device reduces the total weight of the body fluid sample collection device; and the pleural effusion sample can be timely separated and delivered for inspection. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to make the technical solutions in the embodiments of the present application or the prior art clearer, the accompanying drawings that need to be used in the embodiments or prior art description will be briefly described below. Obviously, the accompanying drawings in the following description are only exemplary, and for those skilled in the art, other drawings can be obtained from the provided drawings without any creative effort.

[0016] Figure 1 is a front view of a body fluid sample collection device for ascites;

[0017] Figure 2 is a structural schematic view of a body fluid sample collection device for ascites;

[0018] Figure 3 is a sectional view of a body fluid sample collection device for ascites;

[0019] Figure 4 is a front view of a viscosity control part of the present application;

[0020] Figure 5 is a sectional view of a viscosity control part of the present application;

[0021] Figure 6 is a structural schematic view of a first casing of a centrifugal part of the present application;

[0022] Figure 7 is a sectional view of a first casing of a centrifugal part of the present application;

[0023] Figure 8 is a structural schematic view of a second casing of a centrifugal part of the present application;

[0024] Figure 9 is a sectional view of a second casing of a centrifugal part of the present application;

[0025] Figure 10 is a structural schematic view of a cover plate part of a second casing of the present application;

[0026] Figure 11 is a sectional view of a cover plate part of a second casing of the present application.

[0027] Explanation of reference numerals: 1 suction line; 2 viscosity control part; 21 first sealing section; 21a first electrode; 22 first branch pipe opening; 23 conical pipe; 24 second branch pipe opening; 25 second sealing section; 25a second electrode; 25b one-way valve; 26 sample outlet end; 27 conductive coating; 3 centrifugal part; 31 first casing; 31a sample inlet end; 31b sediment outlet end; 31c cell screen; 32 second casing; 32a cover plate part; 32b collection part; 32c centrifugal power device; 32d sediment inlet end; 4 body fluid bottle. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present application will be clearly and completely described with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments of the present application.

[0029] Embodiment one

[0030] In combination with Figures 1 to 3 As shown in the figure, a body fluid sample collection device for ascites includes a suction line 1 and a body fluid bottle 4 connected with the suction line 1, a pretreatment device is arranged between the body fluid bottle 4 and the suction line 1, the pretreatment device includes a viscosity control part 2, the viscosity control part 2 is provided with a tapered tube 23, the inner diameter of the tapered tube 23 near one end of the suction line 1 is smaller than the inner diameter of the tapered tube 23 far from the one end of the suction line 1, both ends of the tapered tube 23 are provided with openable and closable branch pipe openings, the branch pipe openings include a first branch pipe opening 22 and a second branch pipe opening 24, the first branch pipe opening 22 is closer to the suction line 1 than the second branch pipe opening 24. During the suction process, the high-viscosity ascites sample passes through the tapered tube 23 with gradually increasing inner diameter, and the increase of the cross-sectional area through which the ascites sample passes can effectively avoid the blockage of the ascites sample in the pipeline; the two ends of the tapered tube 23 are provided with openable and closable branch pipe openings, and the anticoagulant can be added through the first branch pipe opening 22 according to the viscosity of the ascites sample, the anticoagulant and the ascites sample are fully mixed in the tapered tube 23, the ascites sample in the tapered tube 23 is diluted, and the anticoagulant is added through the second branch pipe opening 24 before and / or after use, the anticoagulant fully washes or seals the tapered tube 23 and the pipeline connected with the suction line 1, and the residual ascites sample in the pipeline is avoided to cause the blockage of the pipeline. The above-mentioned anticoagulant can be normal saline, heparin saline, etc.

[0031] In combination with Figure 4 and Figure 5 As shown in the figure, the viscosity control part 2 is provided with two electrodes, and there is a pressure difference between the electrodes, and the inner wall of the tapered tube 23 is provided with a conductive coating 27. The end of the viscosity control part 2 close to the suction line 1 is provided with a first sealing section 21, the first sealing section 21 connects the tapered tube 23 and the suction line 1, the inside of the first sealing section 21 is provided with a first electrode 21a, the end of the viscosity control part 2 close to the end far from the suction line 1 is provided with a second sealing section 25, the second sealing section 25 connects the viscosity control part 2 and a centrifugal part 3, the second sealing section 25 is provided with a second electrode 25a and a sample outlet end 26, and there is a pressure difference between the first electrode 21a and the second electrode 25a.

[0032] The inner wall of the conical tube 23 is coated with an electrically conductive coating 27, and the electrode of the viscosity control part 2 is applied to the conical tube 23 to form an electric field, and an electroosmotic flow is formed. The electrically conductive coating 27 can be a nano-porous silicon oxide coating, and the electrode is a titanium alloy plated with platinum, and a 1-5V / mm electric field can increase the flow rate of the ascites simulation solution with a viscosity of 1.5-5cP by 30%-50% under other same conditions; at the same time, due to the direct action of the electric field force on the liquid molecules in the ascites simulation solution, the deposition of cell fragments or fibrin in the pipe wall and the clotting during the flow process can be inhibited or even separated. With the above settings, the action time and range of the electroosmotic flow are controlled to avoid the change of the biochemical characteristics of the ascites caused by the electroosmotic flow, and at the same time, the suction pipeline 1 and the pipeline interval between the electrode and the patient can avoid the possible tissue damage caused by the voltage to the patient.

[0033] As shown in Figure 5 , the second sealing section 25 is provided with a one-way valve 25b, and the one-way valve 25b is used to make the internal medium of the pretreatment device flow from the viscosity control part 2 to the centrifugal part 3. Before and / or after the use of the body fluid sample collection device and / or when the pipeline is blocked, it is necessary to inject anticoagulant into the second branch port 24, and the second sealing section 25 provided with the one-way valve can avoid the backflow of the anticoagulant for flushing the pipeline and sealing the pipeline, causing pipeline pollution; and avoid the backflow of the ascites sample centrifuged by the centrifugal screen, causing the repeatedly screened sample to be centrifuged, and avoiding the prolongation of the ascites drainage time.

[0034] As shown in Figure 2 and Figure 6 , the pretreatment device further comprises a centrifugal part 3, and the centrifugal part 3 comprises a first shell 31, and the inner circle of the first shell 31 is provided with a sample inlet end 31a connected with the second sealing section 25. The centrifugal part 3 is provided to screen the mixture in the drained ascites sample, so as to facilitate different biochemical detection and cytological analysis of different components of the drained ascites sample.

[0035] As shown in Figure 3 and Figure 7As shown, the outer ring of the first shell 31 is provided with a clear liquid outlet end connected to the body fluid bottle 4, and the sample inlet end 31a is located below the clear liquid outlet end. A cell screen 31c is provided between the sample inlet end 31a and the clear liquid outlet end. During the centrifugation process, the first shell 31 rotates, and the clear liquid and some small-sized cell debris and other particles are sieved to the upper layer of the sample. The clear liquid and small-sized particles are collected by the body fluid bottle 4, and the precipitate and large-sized cell debris and other particles sink to the bottom of the sample, completing the separation of the pleural and ascites samples. With the above-mentioned arrangement, the cell screen 31c can prevent some precipitate and large-sized debris from being sieved out from the clear liquid outlet end during the early centrifugation process, thereby improving the sample separation rate. In addition, during the centrifugal sieving process, the pleural and ascites fluid will wash the surface of the cell screen 31c, preventing the cell screen 31c from being blocked and requiring frequent cleaning of the cell screen 31c.

[0036] Combine Figure 3 and Figure 8 As shown, the centrifugal part 3 further includes a second shell 32 , and a centrifugal power device 32 c is fixed inside the second shell 32 , and the output end of the centrifugal power device 32 c is connected to the bottom of the first shell 31 .

[0037] like Figure 9 As shown, the first housing 31 is provided with a sediment outlet 31b, and the top of the second housing 32 is provided with a sediment inlet 32d, which is connected to the sediment outlet 31b. The sediment outlet 31b is located at the lower portion of the side wall of the first housing 31. The second housing 32 is provided to promptly collect the centrifugally sieved sediment and large-sized particles, and the body fluid bottle 4 is provided to promptly collect the centrifugally sieved clear fluid and small-sized particles. This prevents accumulation of pleural and ascites samples within the first housing 31 and repeated sieving. This reduces the volume of the first housing 31 and the power required by the centrifugal power unit 32c of the first housing 31, facilitates the selection of a miniaturized centrifugal power unit 32c, and reduces the overall weight of the body fluid sample collection device.

[0038] Combine Figure 10 and Figure 11 As shown, the second shell 32 includes two parts, including a collection part 32b and a cover part 32a on top of the collection part 32b. The cover part 32a is provided with a sediment inlet 32d, and the cover part 32a isolates the interior of the collection part 32b from the external environment. With the above arrangement, the collection part 32b of the second shell 32 can be disassembled, separated and replaced, facilitating the timely delivery of the collected sediment and large-particle size parts for inspection, while not delaying the body fluid sample collection device from continuing to process and screen the pleural effusion sample. The shape of the collection part 32b is similar to a culture dish, facilitating the subsequent direct delivery of the collection part 32b for inspection, reducing the risk of sample exposure and contamination during sample transfer. The cover part 32a keeps the interior of the collection part 32b isolated from the external environment, avoiding sample contamination during the collection of the pleural effusion sample.

[0039] A method for using a body fluid sample collection device for pleural effusion, which is suitable for the above-mentioned body fluid sample collection device for pleural effusion. The body fluid sample collection device can add anticoagulant to the viscosity control part 2 through the second branch port 24 before and / or after use and / or when the pipeline is blocked. The body fluid sample collection device can add anticoagulant to the viscosity control part 2 through the first branch port 22 during use. During the suction process, the high-viscosity pleural effusion sample passes through the tapered tube 23 with gradually increasing inner diameter, and the increase in the cross-sectional area of the pleural effusion sample can effectively prevent the pipeline from being blocked by the coagulation of the pleural effusion sample. The tapered tube 23 is provided with openable and closable branch ports at both ends. According to the viscosity of the pleural effusion sample, anticoagulant can be added through the first branch port 22, the anticoagulant and the pleural effusion sample are fully mixed in the tapered tube 23, the pleural effusion sample in the tapered tube 23 is diluted, and before and / or after use, the second branch port 24 is added. The anticoagulant fully washes or seals the pipeline of the tapered tube 23 and the connecting suction pipeline 1, so as to avoid the pipeline blockage caused by the residual pleural effusion sample in the pipeline.

[0040] The present application solves the problem that the suction pipeline 1 is blocked and the accuracy of the microscope is affected due to the coagulation of the high-viscosity pleural effusion during the suction process. The residual coagulation in the pipeline causes pipeline blockage before and after the suction process of the multiple suction of the pleural effusion and the indwelling suction of the pleural effusion, and even the replacement of the indwelling and suction instruments. The present application has the following beneficial effects: the flow rate of the pleural effusion simulation solution under other equivalent conditions is increased by 30%-50%; the liquid molecules in the pleural effusion simulation solution are directly affected by the electric field force, which further inhibits or even separates the deposition of the cell fragments or the fibrin in the pipeline wall and the coagulation during the flow process; the miniaturized centrifugal power device 32c reduces the total weight of the body fluid sample collection device; and the pleural effusion sample can be separated and detected in time.

[0041] The above embodiments and / or implementations are only used to illustrate the preferred embodiments and / or implementations of the present application, and do not limit the embodiments of the present application in any form. Any person skilled in the art can make some changes without departing from the scope of the technical means disclosed in the present application, and other equivalent embodiments can be made, but they should be regarded as the same technology or embodiment as the present application.

Claims

1. A body fluid sample collection device for pleural and ascites, comprising a suction line (1) and a body fluid bottle (4) connected to the suction line (1), characterized in that: A pretreatment device is provided between the body fluid bottle (4) and the suction pipeline (1), the pretreatment device comprising a viscosity control portion (2), the viscosity control portion (2) being provided with a tapered tube (23), the inner diameter of the tapered tube (23) at one end close to the suction pipeline (1) being smaller than the inner diameter of the end away from the suction pipeline (1), both ends of the tapered tube (23) being provided with openable and closable branch pipe openings, the branch pipe openings comprising a first branch pipe opening (22) and a second branch pipe opening (24), the first branch pipe opening (22) being closer to the suction pipeline (1) than the second branch pipe opening (24); The pretreatment device further comprises a centrifugal part (3), the centrifugal part (3) further comprises a second shell (32), the second shell (32) comprises two parts, the parts comprising a collecting part (32b) and a cover part (32a) on top of the collecting part (32b), the cover part (32a) isolating the interior of the collecting part (32b) from the external environment, and the collecting part (32b) of the second shell (32) can be disassembled, separated and replaced; The viscosity control portion (2) is provided with at least two electrodes, a pressure difference exists between the electrodes, and the inner wall of the tapered tube (23) is provided with a conductive coating (27).

2. A body fluid sample collection device for pleural effusion and ascites according to claim 1, characterized in that: The viscosity control part (2) is provided with a first sealing section (21) at one end close to the suction pipeline (1), the first sealing section (21) connects the conical tube (23) and the suction pipeline (1), and a first electrode (21a) is provided inside the first sealing section (21). The viscosity control part (2) is provided with a second sealing section (25) at one end close to the end away from the suction pipeline (1), the second sealing section (25) connects the viscosity control part (2) and the centrifugal part (3), and the second sealing section (25) is provided with a second electrode (25a). There is a pressure difference between the first electrode (21a) and the second electrode (25a).

3. A body fluid sample collection device for pleural effusion and ascites according to claim 2, characterized in that: The centrifugal part (3) comprises a first shell (31), and the inner ring of the first shell (31) is provided with a sample inlet end (31a) connected to the second sealing section (25).

4. A body fluid sample collection device for pleural effusion and ascites according to claim 3, characterized in that: The outer ring of the first shell (31) is provided with a clear liquid outlet end connected to the body fluid bottle (4), the sample inlet end (31a) is located below the clear liquid outlet end, and a cell screen (31c) is provided between the sample inlet end (31a) and the clear liquid outlet end.

5. A body fluid sample collection device for pleural effusion and ascites according to claim 4, characterized in that: The second sealing section (25) is provided with a one-way valve (25b), and the one-way valve (25b) is used to allow the internal medium of the pretreatment device to flow from the viscosity control part (2) to the centrifugal part (3).

6. A body fluid sample collection device for pleural effusion and ascites according to claim 3, characterized in that: A centrifugal power device (32c) is fixed inside the second shell (32), and an output end of the centrifugal power device (32c) is connected to the bottom of the first shell (31).

7. A body fluid sample collection device for pleural effusion and ascites according to claim 6, characterized in that: The first shell (31) is provided with a sedimentation outlet (31b), and the top of the second shell (32) is provided with a sedimentation inlet (32d), the sedimentation inlet (32d) is communicated with the sedimentation outlet (31b), and the sedimentation outlet (31b) is located at the lower part of the side wall of the first shell (31).

8. A body fluid sample collection device for pleural and ascites according to claim 7, characterized in that: The cover plate split (32a) is provided with the sedimentation inlet end (32d).

9. A method for using a body fluid sample collection device for pleural effusion and ascites, applicable to the body fluid sample collection device for pleural effusion and ascites according to any one of claims 1 to 8, characterized in that: The body fluid sample collection device can use the second branch tube port (24) to add anticoagulant to the viscosity control part (2) before and / or after use and / or when the pipeline is blocked. The body fluid sample collection device can use the first branch tube port (22) to add anticoagulant to the viscosity control part (2) during use.

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