Composition containing glassine and application of composition in preparation of product with anti-aging effect

Abstract

The invention belongs to the technical field of cosmetics, and particularly discloses a composition containing botryine and application of the composition in preparation of a product with an anti-aging effect, and the composition is prepared from a fructus forsythiae extract, a clove bud extract and botryine; the mass ratio of the fructus forsythiae extract to the clove bud extract to the botryine is 1: (0.2-1): (0.8-2). The fructus forsythiae extract, the clove bud extract and the vitriol in the composition have a remarkable synergistic effect in the anti-aging aspect, and under the synergistic effect of the fructus forsythiae extract, the clove bud extract and the vitriol, the anti-aging effect is effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to a composition containing hydroxyprolisane and its application in the preparation of products with anti-aging effects. Background Art

[0002] Aging is a natural physiological process of the human body. Due to factors such as free radical accumulation and chronic inflammation, the aging process may be accelerated, leading to a series of aging-related diseases, such as skin aging and cell function decline.

[0003] In recent years, with the increasing demand for anti-aging, many anti-aging products and methods have been proposed, including the application of plant extracts, natural compounds, and traditional Chinese medicine compound prescriptions. Hydroxyprolisane has anti-inflammatory, antioxidant, and cell-protecting effects, and can effectively slow down the aging process. However, the separate application of hydroxyprolisane in the prior art is relatively limited, and there is a lack of a synergistic formula with other active ingredients.

[0004] In view of this, the present application is proposed. Summary of the Invention

[0005] The present invention provides a composition containing hydroxyprolisane and its application in the preparation of products with anti-aging effects. The forsythia extract, clove bud extract, and hydroxyprolisane in the composition of the present invention have a significant synergistic effect in anti-aging. Under the synergistic effect of the three, the anti-aging effect is effectively improved.

[0006] The present invention solves its technical problems by adopting the following technical solutions: A composition containing hydroxyprolisane, comprising forsythia extract, clove bud extract, and hydroxyprolisane; the mass ratio of the forsythia extract, clove bud extract, and hydroxyprolisane is 1:(0.2 - 1):(0.8 - 2).

[0007] The forsythia extract, clove bud extract and pro-xylane (also known as hydroxypropyltetrahydropyrantriol) in the composition of the present invention have a significant synergistic effect in anti-aging. Under the synergistic effect of the three, the anti-aging effect is effectively improved. Among them, the forsythia extract, clove bud extract and pro-xylane all have excellent antioxidant effects, can effectively scavenge free radicals, reduce oxidative stress, and reduce oxidative damage to cells and tissues during the aging process. The forsythia extract inhibits the release of inflammatory factors (such as TNF-α, IL-6), and at the same time has a good inhibitory effect on pathogens (such as Staphylococcus aureus). The clove bud extract can inhibit the activity of 5α-reductase, regulate androgen levels, and improve acne and enlarged pores caused by hormonal imbalance; at the same time, it inhibits the synthesis of inflammatory mediator PGE2, forming a double antibacterial and anti-inflammatory barrier with forsythia. The clove bud and pro-xylane delay the aging process of cells by regulating aging-related genes and promoting autophagy. At the specific mass ratio of the present invention, the synergistic effect of the three is further enhanced, jointly improving the anti-aging effect of the composition.

[0008] As a preferred embodiment of the present invention, the mass ratio of the forsythia extract, clove bud extract and pro-xylane is 1:(0.4~0.8):(1.2~1.6).

[0009] As a preferred embodiment of the present invention, the preparation method of the clove bud extract is as follows: (1) Add clove bud powder, cellulase, pectinase, and ficin to water, and carry out enzymatic hydrolysis at 48~55°C for 3~7h to obtain an enzymatic hydrolysate; (2) Add the enzymatic hydrolysate to anhydrous ethanol, let it stand, filter, and centrifuge to obtain a supernatant; (3) Load the supernatant onto D101 macroporous adsorption resin at a flow rate of 0.5~2BV / H, with an elution rate of 0.5~2BV / h. First wash with deionized water for 1~2BV, then wash with 70~85wt% ethanol solution for 0.5~2BV, collect the ethanol solution eluate, and dry to obtain the clove bud extract.

[0010] As a preferred embodiment of the present invention, the mass ratio of the clove bud powder, cellulase, pectinase, ficin, and water is 1:(0.02~0.03):(0.01~0.02):(0.05~0.15):(4~20).

[0011] As a preferred embodiment of the present invention, the volume ratio of the enzymatic hydrolysate to anhydrous ethanol is 1:(2~6).

[0012] As a preferred embodiment of the present invention, the particle size of the clove bud powder is 20~400 mesh.

[0013] As a preferred embodiment of the present invention, the preparation method of the forsythia extract is as follows: (1) Perform supercritical extraction on forsythia powder to obtain an extraction product; (2) Mix the extraction product, n-butanol, and water evenly, add cellulase and pectinase, enzymatically hydrolyze at 45 - 52 °C for 2 - 6 h, filter, and dry to obtain forsythia extract.

[0014] As a preferred embodiment of the present invention, in the supercritical extraction, 70 - 80 wt% ethanol is used as the entrainer, CO2 is used as the extractant, the CO2 flow rate is 60 - 80 mL / min, the extraction temperature is 38 - 42 °C, the extraction pressure is 25 - 27 Mpa, and the extraction time is 60 - 90 min.

[0015] As a preferred embodiment of the present invention, the mass ratio of the extraction product, n-butanol, water, cellulase, and pectinase is 1:(0.5 - 2):(4 - 10):(0.01 - 0.02):(0.01 - 0.02).

[0016] As a preferred embodiment of the present invention, the particle size of the forsythia powder is 20 - 400 mesh.

[0017] The present invention also provides the use of a composition containing hydroxyprolisilane in the preparation of a product with anti-aging effects, wherein the product is a cosmetic, and the dosage form of the cosmetic is an aqueous solution, emulsion, cream, gel, or mask.

[0018] The present invention also provides an anti-aging essence, which includes the following components in weight percentage: 1 - 10% glycerol, 1 - 8% glycerol polyether-26, 0.5 - 2% butanediol, 0.05 - 0.5% xanthan gum, 0.05 - 0.5% p-hydroxyacetophenone, 0.5 - 5% composition containing hydroxyprolisilane, and the balance water; The composition containing hydroxyprolisilane is the composition containing hydroxyprolisilane described above.

[0019] Advantages of the present invention: Forsythia extract, clove bud extract and pro-Xylane in the composition of the present invention have significant synergistic effects in anti-aging. Under the synergistic effect of the three, the anti-aging effect is effectively improved. Among them, forsythia extract, clove bud extract and pro-Xylane all have excellent antioxidant effects, can effectively scavenge free radicals, reduce oxidative stress, and reduce oxidative damage to cells and tissues during the aging process. Forsythia extract inhibits the release of inflammatory factors (such as TNF-α, IL-6), and at the same time has a good inhibitory effect on pathogens (such as Staphylococcus aureus). Clove bud extract can inhibit the activity of 5α-reductase, regulate androgen levels, and improve acne and enlarged pores caused by hormonal imbalance; at the same time, it inhibits the synthesis of inflammatory mediator PGE2, forming a double antibacterial and anti-inflammatory barrier with forsythia. Clove bud and pro-Xylane delay the aging process of cells by regulating aging-related genes and promoting autophagy. At the specific mass ratio of the present invention, the synergistic effect of the three is further enhanced, jointly improving the anti-aging effect of the composition. Detailed implementation mode

[0020] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Apparently, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0021] In the present invention, among the technically characterized described in an open-ended manner, there are closed technical solutions composed of the listed features, as well as open technical solutions including the listed features.

[0022] In the present invention, regarding numerical ranges, unless otherwise specified, the above numerical ranges are considered continuous, and include the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when the range refers to integers, it includes each integer between the minimum and maximum values of the range. In addition, when multiple ranges are provided to describe features or characteristics, these ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0023] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0024] For the reagents or instruments used in the present invention that are not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase. The raw materials used in each comparative ratio and the raw materials used in the parallel experiments of each example are the same commercially available products except as specifically described.

[0025] Example 1 An anti-aging essence, comprising the following components in weight percentage: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance water.

[0026] The composition containing hydroxyproline includes forsythia extract, clove bud extract, and hydroxyproline; the mass ratio of the forsythia extract, clove bud extract, and hydroxyproline is 1:0.4:1.6.

[0027] The preparation method of the clove bud extract is as follows: (1) Crush the clove buds to 50 meshes to obtain clove bud powder; Add the clove bud powder, cellulase, pectinase, and ficin to water, and carry out enzymatic hydrolysis at 50°C for 5 h to obtain an enzymatic hydrolysate; the mass ratio of the clove bud powder, cellulase, pectinase, ficin, and water is 1:0.02:0.02:0.05:10.

[0028] (2) Add the enzymatic hydrolysate to absolute ethanol, let it stand for 12 h, filter, and centrifuge at 5000 rpm for 10 min to obtain a supernatant; the volume ratio of the enzymatic hydrolysate to absolute ethanol is 1:5; (3) Load the supernatant onto D101 macroporous adsorption resin at a flow rate of 1 BV / H, with an elution speed of 1 BV / h. First, wash with deionized water for 1.5 BV, then wash with 80 wt% ethanol solution for 1 BV, collect the ethanol solution eluate, and dry to obtain the clove bud extract.

[0029] The preparation method of the forsythia extract is as follows: (1) Crush the forsythia to 50 meshes to obtain forsythia powder; Add the forsythia powder to a supercritical extraction device for supercritical extraction to obtain an extraction product; the supercritical extraction uses 75 wt% ethanol as an entrainer, CO2 as an extractant, the CO2 flow rate is 70 mL / min, the extraction temperature is 40°C, the extraction pressure is 26 Mpa, and the extraction time is 80 min.

[0030] (2) Mix the extraction product, n-butanol, and water evenly, add cellulase and pectinase, carry out enzymatic hydrolysis at 50°C for 4 h, filter, and dry to obtain the forsythia extract. The mass ratio of the extraction product, n-butanol, water, cellulase, and pectinase is 1:1:5:0.01:0.02.

[0031] Example 2 An anti-aging essence, comprising the following components in weight percentage: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance water.

[0032] The described composition containing hydroxyproline includes forsythia extract, clove bud extract and hydroxyproline; the mass ratio of forsythia extract, clove bud extract and hydroxyproline is 1:0.8:1.2.

[0033] The preparation method of the clove bud extract is as follows: (1) Crush the clove buds to 50 mesh to obtain clove bud powder; Add the clove bud powder, cellulase, pectinase, ficin to water, and carry out enzymatic hydrolysis at 50 °C for 5 h to obtain an enzymatic hydrolysate; the mass ratio of the clove bud powder, cellulase, pectinase, ficin, and water is 1:0.02:0.02:0.05:10.

[0034] (2) Add the enzymatic hydrolysate to absolute ethanol, let it stand for 12 h, filter, and centrifuge at 5000 rpm for 10 min to obtain a supernatant; the volume ratio of the enzymatic hydrolysate to absolute ethanol is 1:5; (3) Load the supernatant onto D101 macroporous adsorption resin at a flow rate of 1 BV / H, with an elution speed of 1 BV / h. First, wash with deionized water for 1.5 BV, then wash with 80 wt% ethanol solution for 1 BV, collect the ethanol solution eluate, and dry to obtain the clove bud extract.

[0035] The preparation method of the forsythia extract is as follows: (1) Crush the forsythia to 50 mesh to obtain forsythia powder; Add the forsythia powder to a supercritical extraction device for supercritical extraction to obtain an extraction product; the supercritical extraction uses 75 wt% ethanol as an entrainer, CO2 as an extractant, the CO2 flow rate is 70 mL / min, the extraction temperature is 40 °C, the extraction pressure is 26 Mpa, and the extraction time is 80 min.

[0036] (2) Mix the extraction product, n-butanol, and water evenly, add cellulase and pectinase, carry out enzymatic hydrolysis at 50 °C for 4 h, filter, and dry to obtain the forsythia extract. The mass ratio of the extraction product, n-butanol, water, cellulase, and pectinase is 1:1:5:0.01:0.02.

[0037] Example 3 An anti-aging essence contains the following components in weight percentages: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance is water.

[0038] The described composition containing hydroxyproline includes forsythia extract, clove bud extract and hydroxyproline; the mass ratio of forsythia extract, clove bud extract and hydroxyproline is 1:0.2:0.8.

[0039] The preparation method of the clove bud extract is as follows: (1) Crush the clove buds to 50 mesh to obtain clove bud powder; Add the clove bud powder, cellulase, pectinase, and ficin into water, and carry out enzymatic hydrolysis at 50°C for 5 h to obtain an enzymatic hydrolysate; the mass ratio of the clove bud powder, cellulase, pectinase, ficin, and water is 1:0.02:0.02:0.05:10.

[0040] (2) Add the enzymatic hydrolysate into absolute ethanol, let it stand for 12 h, filter, and centrifuge at a speed of 5000 rpm for 10 min to obtain a supernatant; the volume ratio of the enzymatic hydrolysate to absolute ethanol is 1:5; (3) Load the supernatant onto D101 macroporous adsorption resin at a flow rate of 1 BV / H, with an elution speed of 1 BV / h. First, wash with deionized water for 1.5 BV, then wash with 80 wt% ethanol solution for 1 BV, collect the ethanol solution eluate, and dry it to obtain the clove bud extract.

[0041] The preparation method of the forsythia extract is as follows: (1) Crush the forsythia to 50 mesh to obtain forsythia powder; Add the forsythia powder into a supercritical extraction device for supercritical extraction to obtain an extraction product; the supercritical extraction uses 75 wt% ethanol as an entrainer, CO2 as an extractant, the CO2 flow rate is 70 mL / min, the extraction temperature is 40°C, the extraction pressure is 26 Mpa, and the extraction time is 80 min.

[0042] (2) Mix the extraction product, n-butanol, and water evenly, add cellulase and pectinase, carry out enzymatic hydrolysis at 50°C for 4 h, filter, and dry to obtain the forsythia extract. The mass ratio of the extraction product, n-butanol, water, cellulase, and pectinase is 1:1:5:0.01:0.02.

[0043] Example 4 An anti-aging essence, comprising the following components in weight percentages: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance is water.

[0044] The composition containing hydroxyproline includes forsythia extract, clove bud extract, and hydroxyproline; the mass ratio of the forsythia extract, clove bud extract, and hydroxyproline is 1:1:2.

[0045] The preparation method of the clove bud extract is as follows: (1) Crush the clove buds to 50 mesh to obtain clove bud powder; Add clove bud powder, cellulase, pectinase, and ficin into water, and enzymatically hydrolyze at 50 °C for 5 h to obtain an enzymatically hydrolyzed solution; the mass ratio of the clove bud powder, cellulase, pectinase, ficin, and water is 1:0.02:0.02:0.05:10.

[0046] (2)Add the enzymatically hydrolyzed solution into absolute ethanol, let it stand for 12 h, filter, and centrifuge at 5000 rpm for 10 min to obtain a supernatant; the volume ratio of the enzymatically hydrolyzed solution to absolute ethanol is 1:5; (3)Load the supernatant onto D101 macroporous adsorption resin at a flow rate of 1 BV / H, with an elution speed of 1 BV / h. First, wash with deionized water for 1.5 BV, then wash with 80 wt% ethanol solution for 1 BV, collect the ethanol solution eluate, and dry to obtain the clove bud extract.

[0047] The preparation method of the forsythia extract is as follows: (1)Crush forsythia to 50 mesh to obtain forsythia powder; Add the forsythia powder into a supercritical extraction device for supercritical extraction to obtain an extraction product; the supercritical extraction uses 75 wt% ethanol as an entrainer, CO2 as an extractant, the CO2 flow rate is 70 mL / min, the extraction temperature is 40 °C, the extraction pressure is 26 Mpa, and the extraction time is 80 min.

[0048] (2)Mix the extraction product, n-butanol, and water evenly, add cellulase and pectinase, enzymatically hydrolyze at 50 °C for 4 h, filter, and dry to obtain the forsythia extract. The mass ratio of the extraction product, n-butanol, water, cellulase, and pectinase is 1:1:5:0.01:0.02.

[0049] Example 5 An anti-aging essence contains the following components in weight percentages: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyprolisilane, and the balance water.

[0050] The composition containing hydroxyprolisilane includes forsythia extract, clove bud extract, and hydroxyprolisilane; the mass ratio of the forsythia extract, clove bud extract, and hydroxyprolisilane is 1:0.4:1.6.

[0051] The preparation method of the clove bud extract is as follows: (1)Crush clove buds to 50 mesh to obtain clove bud powder; Add clove bud powder, cellulase, pectinase, and ficin into water, and enzymatically hydrolyze at 50 °C for 5 h to obtain an enzymatically hydrolyzed solution; the mass ratio of the clove bud powder, cellulase, pectinase, ficin, and water is 1:0.03:0.01:0.15:10.

[0052] (2) Add the enzymolysis solution to absolute ethanol, let it stand for 12 h, filter, centrifuge at 5000 rpm for 10 min to obtain the supernatant; the volume ratio of the enzymolysis solution to absolute ethanol is 1:5. (3) Load the supernatant onto D101 macroporous adsorption resin at a flow rate of 1 BV / H with an elution rate of 1 BV / h. First, wash with deionized water for 1.5 BV, then wash with 80 wt% ethanol solution for 1 BV, collect the ethanol solution eluate, and dry to obtain the clove bud extract.

[0053] The preparation method of the forsythia extract is as follows: (1) Crush forsythia to 50 meshes to obtain forsythia powder. Add the forsythia powder to a supercritical extraction device for supercritical extraction to obtain an extraction product; 75 wt% ethanol is used as the entrainer, CO2 is used as the extractant, the CO2 flow rate is 70 mL / min, the extraction temperature is 40 °C, the extraction pressure is 26 Mpa, and the extraction time is 80 min.

[0054] (2) Mix the extraction product, n-butanol, and water evenly, add cellulase and pectinase, enzymolyze at 50 °C for 4 h, filter, and dry to obtain the forsythia extract. The mass ratio of the extraction product, n-butanol, water, cellulase, and pectinase is 1:1:5:0.02:0.01.

[0055] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain forsythia extract (the total amount of the composition remains unchanged), and the others are the same.

[0056] An anti-aging essence contains the following components in weight percentages: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyprolisilane, and the balance is water.

[0057] The composition containing hydroxyprolisilane includes clove bud extract and hydroxyprolisilane; the mass ratio of the clove bud extract to hydroxyprolisilane is 0.4:1.6.

[0058] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not contain hydroxyprolisilane (the total amount of the composition remains unchanged), and the others are the same.

[0059] An anti-aging essence contains the following components in weight percentages: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition, and the balance is water.

[0060] The composition includes forsythia extract and clove bud extract; the mass ratio of the forsythia extract to the clove bud extract is 1:0.4.

[0061] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain clove bud extract (the total amount of the composition remains unchanged), and the others are the same.

[0062] An anti-aging essence includes the following components in weight percentages: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance is water.

[0063] The composition containing hydroxyproline includes forsythia extract and hydroxyproline; the mass ratio of the forsythia extract to the hydroxyproline is 1:1.6.

[0064] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that the mass ratio of the forsythia extract, the clove bud extract and the hydroxyproline in Comparative Example 4 is not within the scope of the present invention, and the others are the same.

[0065] An anti-aging essence includes the following components in weight percentages: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance is water.

[0066] The composition containing hydroxyproline includes forsythia extract, clove bud extract and hydroxyproline; the mass ratio of the forsythia extract, the clove bud extract and the hydroxyproline is 1:2:0.5.

[0067] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that the mass ratio of the forsythia extract, the clove bud extract and the hydroxyproline in Comparative Example 5 is not within the scope of the present invention, and the others are the same.

[0068] An anti-aging essence includes the following components in weight percentages: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance is water.

[0069] The composition containing hydroxyproline includes forsythia extract, clove bud extract and hydroxyproline; the mass ratio of the forsythia extract, the clove bud extract and the hydroxyproline is 1:0.1:4.

[0070] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 uses an equal amount of bisabolol to replace the forsythia extract, and the others are the same.

[0071] An anti-aging essence, comprising the following components in percentage by weight: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance being water.

[0072] The composition containing hydroxyproline includes bisabolol, clove bud extract and hydroxyproline; the mass ratio of bisabolol, clove bud extract and hydroxyproline is 1:0.4:1.6.

[0073] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 uses an equal amount of ergothioneine to replace the clove bud extract, and the others are the same.

[0074] An anti-aging essence, comprising the following components in percentage by weight: 4% glycerol, 3% glycerol polyether-26, 1% butanediol, 0.2% xanthan gum, 0.4% p-hydroxyacetophenone, 2% composition containing hydroxyproline, and the balance being water.

[0075] The composition containing hydroxyproline includes forsythia extract, ergothioneine and hydroxyproline; the mass ratio of forsythia extract, ergothioneine and hydroxyproline is 1:0.4:1.6.

[0076] Test Example 1. The antioxidant performance is the scavenging rate of DPPH according to T / SHRH 006-2018 "Cosmetics - Free Radical (DPPH) Scavenging Experiment Method".

[0077] 2. Select 120 volunteers, randomly divide them into 12 groups with 10 people in each group, and use the essence of the examples and comparative examples respectively. After cleansing their faces in the morning and evening every day, the volunteers use the essence of the examples and comparative examples, 2 mL each time, and use it continuously for 28 days.

[0078] The criteria for including volunteers are: aged 25 - 32 years old, female; those who have not used hormonal drugs and immunosuppressants in the past month; those who have not participated in other clinical trials at the test site now or in the past 30 days; those who can cooperate well with the trial; those who can read and understand all the contents of the informed consent form and voluntarily sign the informed consent form; those who agree not to use any cosmetics, drugs and health products that have an impact on the results during the trial.

[0079] Exclusion criteria: Those with open wounds or erosions; Those with highly allergic constitutions; Those with a history of cosmetics allergy; Pregnant, lactating women or women who intend to become pregnant during the test; Those with severe heart, liver, and kidney function impairments and severe immune function deficiencies; Those with mental illnesses, severe endocrine diseases, and those taking oral contraceptives; Those who participated in a drug clinical trial or other trials within 30 days, or those who took systemic medications that may affect the test results within the past week; Those who used oral and topical beauty products that may affect the test results within 2 weeks; Those whom the researcher deems unfit to participate in this test.

[0080] Discontinuation and withdrawal criteria: During the trial, the test sample was not applied as required or the follow-up records were not made as required; Suffering from a serious illness or experiencing serious adverse reactions during use.

[0081] The Corneometer MPA580 was used to detect the skin elasticity of the volunteers before and 28 days after use (where the larger the detected value, the better the skin elasticity), and the change rate (%) of skin elasticity was calculated and recorded. The calculation method of the change rate of skin elasticity is: Change rate (%) = [(Skin elasticity before use - Skin elasticity after use) / Skin elasticity before use] 100%.

[0082] The skin rapid three-dimensional imaging system PRIMOS was used to detect the skin wrinkles of the volunteers before and 28 days after use (where the smaller the detected value, the fewer the skin wrinkles), and the change rate (%) of skin wrinkles was calculated and recorded. The calculation method of the change rate of skin wrinkles is: Change rate (%) = [(Skin wrinkles after use - Skin wrinkles before use) / Skin wrinkles before use] 100%.

[0083] Table 1

[0084] As can be seen from Table 1, the composition described in the present invention has excellent anti-aging effects.

[0085] By comparing Example 1 with Comparative Examples 1 - 7, it can be seen that the forsythia extract, clove bud extract, and pro-xylane described in the present invention have a significant synergistic effect in anti-aging.

[0086] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition containing hydroxyproline, characterized in that, It includes forsythia extract, clove bud extract and hydroxyproline; the mass ratio of the forsythia extract, clove bud extract and hydroxyproline is 1:(0.2~1):(0.8~2).

2. The composition containing hydroxproline according to claim 1, wherein The mass ratio of the forsythia extract, clove bud extract and hydroxyproline is 1:(0.4~0.8):(1.2~1.6).

3. The composition containing hydroxyproline as claimed in claim 1, wherein The preparation method of the clove bud extract is as follows: (1) Add clove bud powder, cellulase, pectinase and ficin into water, and carry out enzymatic hydrolysis at 48~55°C for 3~7h to obtain an enzymatic hydrolysate; (2) Add the enzymatic hydrolysate into absolute ethanol, let it stand, filter, and centrifuge to obtain a supernatant; (3) Load the supernatant onto D101 macroporous adsorption resin at a flow rate of 0.5~2BV / H, with an elution rate of 0.5~2BV / h. First, wash with deionized water for 1~2BV, then wash with 70~85wt% ethanol solution for 0.5~2BV, collect the ethanol solution eluate, and dry it to obtain the clove bud extract.

4. The composition containing hydroxyproline tris (tetra - isopropyl diphosphite) according to claim 3, characterized in that, The mass ratio of the clove bud powder, cellulase, pectinase, ficin and water is 1:(0.02~0.03):(0.01~0.02):(0.05~0.15):(4~20).

5. The composition containing hydroxproline according to claim 3, wherein The volume ratio of the enzymatic hydrolysate to absolute ethanol is 1:(2~6).

6. The composition containing hydroxyproline trisaccharide according to claim 1, wherein The preparation method of the forsythia extract is as follows: (1) Carry out supercritical extraction on forsythia powder to obtain an extraction product; (2) Mix the extraction product, n-butanol and water evenly, add cellulase and pectinase, carry out enzymatic hydrolysis at 45~52°C for 2~6h, filter and dry to obtain the forsythia extract.

7. The composition containing hydroxyproline trisaccharide according to claim 6, wherein In the supercritical extraction, 70~80wt% ethanol is used as an entrainer, CO2 is used as an extraction agent, the CO2 flow rate is 60~80mL / min, the extraction temperature is 38~42°C, the extraction pressure is 25~27Mpa, and the extraction time is 60~90min.

8. The composition containing hydroxyproline as claimed in claim 6, characterized in that, The mass ratio of the extraction product, n-butanol, water, cellulase and pectinase is 1:(0.5~2):(4~10):(0.01~0.02):(0.01~0.02).

9. Use of the composition containing hydroxproline in the preparation of a product with anti-aging effect according to any one of claims 1 to 8, characterized in that, The product is a cosmetic, and the dosage form of the cosmetic is an aqueous solution, emulsion, cream, gel or mask.

10. An anti-aging essence, characterized in that, It includes the following components in weight percentage: 1~10% glycerin, 1~8% glycerol polyether-26, 0.5~2% butanediol, 0.05~0.5% xanthan gum, 0.05~0.5% p-hydroxyacetophenone, 0.5~5% composition containing hydroxyproline, and the balance is water; The composition containing hydroxyproline is the composition containing hydroxyproline according to any one of claims 1~8.

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