Preparation dressing containing isodecursolactone and application of preparation dressing
The transparent gel-like dressing prepared by isophilaproliferative, ethanol, propylene glycol, and sodium hyaluronate solves the safety and environmental friendliness of existing sunscreens, and achieves effective protection and repair of UVB-induced skin UV irradiation damage.
Patent Information
- Application Number
- CN202510551106.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-29
- Publication Date
- 2025-07-11
AI Technical Summary
Existing sunscreens have problems such as insufficient safety, poor application, color and harmful to the marine biological environment, and cannot effectively protect UVB from skin damage caused by UV rays.
Isoporaprolactone is used as the core component, combined with ethanol, propylene glycol, and sodium hyaluronate to prepare a clear gel-like dressing for preventing or treating UVB-induced skin UV irradiation damage.
It provides a sunscreen preparation with good safety, comfort, beauty and no environmental hazards, which effectively reduces the formation of skin erythema, desquamation and scabs after UVB exposure, and protects the skin dermis and epidermal structure.
Smart Images
Figure CN120284758A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of biomedical technologies, and particularly to a preparation dressing containing imperatorin and its applications. Background Art
[0002] Skin ultraviolet (UV) irradiation injury (sunburn) is an acute skin injury caused by UV radiation, which is specifically manifested as symptoms such as skin redness, pain, desquamation, peeling, etc. UV is divided into: UVA (320 - 400 nm), UVB (280 - 320 nm), and UVC (100 - 280 nm) according to wavelength. UVC is blocked by the atmospheric ozone layer, and only UVA and UVB can actually act on the skin to cause damage. Among them, UVB is the main part that induces skin erythema and causes acute skin injury, and can further induce diseases such as skin cancer, and the damage is much greater than that of UVA.
[0003] Sun protection agents achieve the immediate sun protection effect through mechanisms such as absorption, reflection, or refraction. Common components are titanium dioxide and zinc oxide. Daily use of sun protection agents for anti-ultraviolet irradiation protection plays an important role in preventing sunburn. Considering the impact of UVB on skin beauty and health, sun protection occupies an important position in beauty makeup, skin care, and clinical treatment. Therefore, finding a suitable protective preparation dressing has become an urgent task.
[0004] Over the years, sun protection preparations have made great progress in terms of accessibility, acceptance, and effectiveness. However, current sun protection preparations still face problems and challenges such as insufficient safety, poor aesthetics, poor comfort, and harm to the marine biological environment. Specifically, in the classification rules of sun protection preparations announced by the US FDA, currently only two components, titanium dioxide and zinc oxide, are designated as Class I "GRASE" (Generally Recognized as Safe and Effective). However, titanium dioxide and zinc oxide rely on their colored particles to scatter, reflect, and absorb ultraviolet light to achieve sun protection. At the same time, the aggregation of colored particles on the skin means that current such sun protection agents often leave white marks on the skin of users. Especially when users sweat due to outdoor activities or hot weather, the significantly flowing white marks on the skin surface are unacceptable to many users. At the same time, a variety of sun protection components, including octyl methoxycinnamate, octocrylene, and triclosan, have been proven to seriously harm the marine biological environment, resulting in restrictions on the usage scenarios.
[0005] Therefore, researchers and the industry are eagerly looking forward to the birth and application of sun protection preparations with better safety, dressing feeling, colorless, and friendly to the marine environment, which has shifted their attention to natural ingredients of plant origin. How to obtain a sun protection preparation of plant origin that can improve skin ultraviolet irradiation injury caused by UVB has become a problem to be solved. Summary of the Invention
[0006] The present invention aims to develop a new type of preparation dressing with the plant-derived active ingredient - isopimpinellin as the core. To solve the problems of poor safety, poor application feeling, coloration, and harm to the marine biological environment of existing sunscreen agents, with a view to providing a safer, more comfortable, more aesthetic and environmentally friendly sunscreen preparation dressing in the field of skin care and sunscreen treatment, and providing an applicable product for the prevention and treatment of skin ultraviolet irradiation damage caused by UVB.
[0007] To achieve the above object, the specific technical solutions adopted by the present invention are as follows:
[0008] In the first aspect, the present invention provides a preparation dressing containing isopimpinellin, which is prepared by using isopimpinellin as the key ingredient and combining excipients such as ethanol, propylene glycol, and sodium hyaluronate. The isopimpinellin is a furanocoumarin, which has been isolated from different medicinal plants and can be directly purchased. Its structural formula is shown as follows:
[0009]
[0010] Furthermore, the content of each component of the preparation excipients includes but is not limited to the following preferred scheme: isopimpinellin 2%, absolute ethanol 40%, propylene glycol 40%, and sodium hyaluronate 18%.
[0011] Furthermore, the preparation dressing is in the form of a transparent gel and is a topical preparation dressing for the skin, which can solve the coloration problem of existing sunscreen agents, and has certain ductility and fluidity, can be well attached to the skin surface, and has a good application feeling.
[0012] Furthermore, the preparation method of the preparation dressing is as follows: mix each component at room temperature and stir evenly to obtain the preparation dressing. The preparation of the preparation dressing of the present invention does not require complex equipment and condition control, is simple to operate, and has a low processing cost.
[0013] In the second aspect, the present invention provides the application of the above-mentioned preparation dressing containing isopimpinellin as a drug for preventing or treating skin ultraviolet irradiation damage caused by UVB.
[0014] Furthermore, the application method is to apply the preparation dressing containing isopimpinellin to the skin surface before light (including UVB) irradiation.
[0015] Furthermore, the skin ultraviolet irradiation damage caused by UVB includes the damage of the dermis and epidermis of the skin caused by UVB. Among them, the epidermal damage includes skin redness, erythema, pain, burning, desquamation, peeling, and increased epidermal thickness; the dermal damage includes sebaceous gland atrophy, abundance and density damage of dermal collagen fibers.
[0016] Thirdly, the present invention provides the use of isopimpinellin in the preparation of a drug for preventing or treating skin damage caused by UVB-induced ultraviolet irradiation, and the drug takes isopimpinellin as a key component.
[0017] Furthermore, the drug further comprises excipients, and the excipients include ethanol, propylene glycol, and sodium hyaluronate.
[0018] The present invention has the following beneficial effects:
[0019] 1. The present invention takes isopimpinellin as the main component, and by using the combination of excipients such as ethanol, propylene glycol, and sodium hyaluronate, a novel preparation dressing containing isopimpinellin is prepared, and a skin sunscreen agent with plant active ingredients as the core is developed, which has good safety and is environmentally friendly.
[0020] 2. Based on the UVB-induced skin ultraviolet irradiation damage model of BALb / c mice, the present invention clarifies the sunscreen and skin care effects of this novel preparation dressing containing isopimpinellin, which can reduce skin erythema, desquamation damage and scab formation after UVB irradiation, relieve abnormal epidermal thickening and protect dermal elastic fibers and collagen fibers.
[0021] 3. In the preparation dressing of the present invention, isopimpinellin has good solubility in the combination of excipients such as ethanol, propylene glycol, and sodium hyaluronate, showing a uniform and transparent gel-like state, having certain ductility and fluidity, and can be well attached to the skin surface, with a good dressing feeling, and at the same time solves the problem of color of existing sunscreens. Description of the Drawings
[0022] Figure 1 : Flow chart of the method in Example 1.
[0023] Figure 2 : Flow chart of the test experiment on the effect of the preparation dressing in Example 1.
[0024] Figure 3 : Appearance diagram of the preparation dressing in Example 1.
[0025] Figure 4 : Diagram showing the fluidity and viscosity of the preparation dressing in Example 1.
[0026] Figure 5 : Diagram showing the preparation dressing applied to the skin surface of mice in Example 1.
[0027] Figure 6 : Apparent diagram of the mice in Example 1 from 0 to 7 days after being treated with the preparation dressing and UVB.
[0028] Figure 7 : HE staining picture (left) of the tissue section of the mice in Example 1 after being treated with the preparation dressing and UVB and the epidermal thickness result (right).
[0029] Figure 8 : Masson staining pictures (left) and collagen fiber area results (right) of tissue sections of mice after treatment with the preparation dressing and UVB in Example 1.
[0030] Figure 9 : Resorcinol basic fuchsin staining pictures (left) and collagen fiber area results (right) of tissue sections of mice after treatment with the preparation dressing and UVB in Example 1. Detailed implementation manners
[0031] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
[0032] Example 1
[0033] This example provides a preparation method and effect test of a novel preparation dressing containing marmesin. The flow chart of the overall method is as Figure 1 shown, and the flow chart of the effect test experiment is as Figure 2 shown. The specific process is as follows:
[0034] 1. Preparation of the preparation dressing
[0035] Prepare a preparation dressing containing marmesin according to the formula in Table 1 below as the experimental group sample of 2% high-concentration marmesin. The experimental group samples of 1.5% medium concentration and 1% low concentration of marmesin are obtained by diluting the experimental group sample of 2% high-concentration marmesin with a matrix (anhydrous ethanol + propylene glycol + sodium hyaluronate). The blank control group and the UVB experimental group samples do not contain marmesin. Each component is mixed at room temperature and stirred evenly. The appearance of the prepared preparation dressing is as Figures 3-4 shown. It is in the form of a transparent gel, with certain ductility and fluidity. After depilating the back skin of mice, apply this preparation dressing, as Figure 5 shown. It can be seen that this preparation dressing can adhere well to the surface of the mouse skin and has a good dressing feeling. Table 1 Composition and ratio of the preparation dressing containing marmesin
[0036]
[0037] 2. Experimental animals
[0038] 30 6-week-old SPF-grade BALb / c mice are raised under standard conditions (22 ± 3 °C, relative humidity 55 ± 5%, standard light environment, free access to food and water).
[0039] 3. Grouping and administration
[0040] After 1 week of adaptive feeding of the mice, 5 mice in each group were randomly divided into 6 groups. One day before the experiment, the hair on the backs of the mice was removed using depilatory cream. The UVB lamp was preheated for 10 min before irradiation, and the UVB irradiator showed that the UVB intensity was stable at (2.0 ± 0.2) mW. Except for the normal control group, 30 min after administration to the remaining groups, the mice were placed under the UVB irradiator for a single continuous irradiation until the total irradiation dose reached 400 mJ / cm 2 . Observation was continued for 7 days, and the appearance was photographed and recorded with a camera. The specific grouping and treatment plan are shown in Table 2 below.
[0041] Table 2 Experimental grouping
[0042]
[0043] 4. Tissue section preparation
[0044] 4.1 Specimen collection and fixation
[0045] After 7 days of observation of the administration and modeling treatment, the mice were anesthetized and sacrificed, and the skin tissue in the center of the back of each experimental mouse was carefully dissected. Each piece of skin was cut into an area of about 1 cm × 1 cm, washed clean with PBS buffer, quickly placed in a 15 mL centrifuge tube, added 3 mL of 4% paraformaldehyde for fixation, and stored at 4°C. After the tissue was fixed, the skin tissues of each group of mice were taken out of the fixative, trimmed to make the edges flush, labeled, and placed in a dehydration box.
[0046] 4.2 Dehydration and wax infiltration
[0047] The dehydration box was placed in a hanging basket, and gradient ethanol was used for dehydration in turn. 75% ethanol for 4 h → 85% ethanol for 2 h → 90% ethanol for 2 h → 95% ethanol for 1 h → absolute ethanol for 30 min (2 times) → ethanol-benzene for 10 min → xylene for 10 min (2 times) → 65°C paraffin for 1 h (3 times).
[0048] 4.3 Embedding and sectioning
[0049] After wax infiltration, it was placed in an embedding machine for embedding. Liquid paraffin was poured into the embedding frame, the tissue was quickly taken out and placed in the embedding frame, and labeled. It was placed on a -20°C freezing table until the paraffin cooled and solidified. The wax block was taken out and appropriately trimmed. After trimming, it was placed on a paraffin slicer, and the tissue wax block was sliced at a thickness of 4 μm. The slices were taken out and placed on the water surface of a 40°C constant temperature water bath to flatten the tissue. The tissue was picked up with a glass slide and baked at 60°C for 2 h, and stored at room temperature for later use.
[0050] 5. HE staining
[0051] The paraffin sections of the skin tissues of each group of mice were dewaxed successively. Xylene for 20 min (twice) → absolute ethanol for 5 min (twice) → 75% ethanol for 5 min. Hematoxylin staining for 5 min, differentiation with differentiating solution, and bluing with bluing solution. Dehydration with gradient ethanol again. 85% ethanol for 5 min → 95% ethanol for 5 min. Eosin staining for 5 min, dehydration and mounting. Absolute ethanol for 5 min (thrice) → xylene for 5 min (twice) → mounting with neutral gum. Observed under an optical microscope and images were collected.
[0052] 6. Masson staining
[0053] The paraffin sections of the skin tissues of each group of mice were dewaxed successively. Xylene for 20 min (twice) → absolute ethanol for 5 min (twice) → 75% ethanol for 5 min. Stained with potassium dichromate overnight and rinsed with running water. Mixed iron hematoxylin solutions A and B in a 1:1 ratio to prepare iron hematoxylin staining solution. Iron hematoxylin staining for 3 min, washed with tap water, differentiated with differentiating solution, washed with tap water, blued with bluing solution, and rinsed with running water. Ponceau acid fuchsin staining for 10 min and rinsed with running water. Phosphomolybdic acid staining for 3 min. Aniline blue staining for 6 min. Differentiated with 1% glacial acetic acid and dehydrated with absolute ethanol for 5 min (thrice). Cleared with xylene for 5 min and mounted with neutral gum. Observed under an optical microscope and images were collected.
[0054] 7. Resorcinol basic fuchsin staining
[0055] The paraffin sections of the skin tissues of each group of mice were dewaxed successively. Xylene for 20 min (twice) → absolute ethanol for 5 min (twice) → 75% ethanol for 5 min. Mixed potassium permanganate and sulfuric acid in a 1:1 ratio to prepare combined acidified potassium permanganate solution and rinsed with running water. Bleached with acid solution for 2 min and immersed in 95% ethanol. Resorcinol basic fuchsin staining for 4 h. Differentiated the background with hydrochloric acid alcohol. Mixed picric acid and acid fuchsin in a 9:1 ratio to prepare VG staining solution and VG staining for 3 min. Dehydrated with absolute ethanol for 5 min (thrice). Cleared with xylene for 5 min and mounted with neutral gum. Observed under an optical microscope and images were collected.
[0056] 8. Experimental results of effect test
[0057] The skin appearance of mice after treatment with the preparation dressing and UVB was as Figure 6 shown. Compared with the Control group, after UVB irradiation, obvious erythema and desquamation appeared on the dorsal skin of mice, and it gradually thickened and crusted. After pretreatment with each concentration (1%, 1.5%, 2%) of marmesin, the formation of erythema desquamation injury and crusts decreased significantly with the increase of the concentration of the intervention drug.
[0058] The results of HE staining of tissue sections were as Figure 7As shown, compared with the Control group, the epidermis of the dorsal skin of mice in the UVB group was significantly thickened, covered with scabs formed after pathological photoaging damage, and the boundary between the epidermis and dermis was blurred and chaotic. Marmesin intervention effectively reduced the increase in epidermal thickness caused by UVB irradiation and protected the normal structure and morphology of the skin.
[0059] The results of Masson staining of tissue sections are as Figure 8 shown. Compared with the Control group, the abundance and density of blue collagen fibers in the UVB group were significantly reduced, and the abnormal epidermal structure was intertwined with sparse and broken collagen fibers. After pretreatment with marmesin, the collagen fiber damage induced by UVB was significantly improved.
[0060] The results of resorcinol basic fuchsin staining of tissue sections are as Figure 9 shown. Compared with the Control group, the content of red collagen fibers in the dermis of the UVB irradiation group was significantly reduced, and only some fragmented fiber segments were visible. In each concentration of marmesin group, the collagen fiber damage in the dermis induced by UVB was significantly improved. The results showed that marmesin could effectively protect the collagen fibers in the skin dermis from damage induced by UVB.
[0061] In summary, after pretreatment with the preparation dressings containing marmesin at various concentrations (1%, 1.5%, 2%), the injuries such as erythema, desquamation and scabs caused by UVB irradiation were significantly reduced with the increase of the concentration of the intervention drug; HE staining of tissue sections showed that pretreatment with marmesin effectively reduced the increase in epidermal thickness and sebaceous gland atrophy caused by UVB irradiation; in Masson and resorcinol basic fuchsin staining, it was visible that after pretreatment with marmesin, the damage to the abundance and density of collagen fibers induced by UVB was significantly improved, and the arrangement of collagen fibers was significantly neater and denser than that in the UVB group. It can be concluded that the novel preparation dressing containing marmesin of the present invention can protect the skin dermis and epidermis from damage caused by UVB.
[0062] This specific embodiment is only an explanation of the present invention and not a limitation thereof. Any changes made by those skilled in the art after reading the specification of the present invention will be protected by the patent law as long as they are within the scope of the claims of the present invention.
Claims
1. A preparation dressing containing nodakenetin, characterized in that, It is prepared by using nodakenetin as a key component and combining excipients such as ethanol, propylene glycol, and sodium hyaluronate.
2. The preparation dressing containing nodakenetin according to claim 1, wherein The preparation dressing is in the form of a transparent gel and is a dressing for topical skin preparation.
3. The preparation dressing containing nodakenetin according to claim 1, characterized in that, The preparation method is as follows: Mix each component at room temperature and stir evenly to obtain the preparation dressing.
4. Use of the preparation dressing containing nodakenetin according to any one of claims 1-3 as a drug for preventing or treating skin ultraviolet irradiation damage caused by UVB.
5. The application according to claim 4, wherein The application method is to apply the preparation dressing containing nodakenetin to the skin surface before light irradiation.
6. The application according to claim 4, characterized in that, The skin ultraviolet irradiation damage caused by UVB includes skin dermal and epidermal damage caused by UVB.
7. The application according to claim 6, wherein Epidermal damage includes skin redness, erythema, pain, burning, desquamation, peeling, and increased epidermal thickness.
8. The application according to claim 6, wherein Dermal damage includes sebaceous gland atrophy and damage to the abundance and density of dermal collagen fibers.
9. Use of imperatorin in the preparation of a drug for preventing or treating skin ultraviolet irradiation damage caused by UVB, characterized in that, The drug uses nodakenetin as a key component.
10. The application according to claim 9, characterized in that, The drug further includes excipients, and the excipients include ethanol, propylene glycol, and sodium hyaluronate.