An anti-wrinkle and firming composition, its preparation method and application

Abstract

This invention belongs to the field of cosmetic technology, specifically relating to an anti-wrinkle and firming composition, its preparation method, and its application. The composition comprises the following raw materials in the indicated weight percentages: 0.1-0.2% acetyl hexapeptide-8, 0.3-0.5% *Gynostemma pentaphyllum* polysaccharide extract, 0.051-0.069% ethylhexylglycerin, 0.53-0.55% phenoxyethanol, 25-35% glycerin, and the balance being water. In this invention, *Gynostemma pentaphyllum* algae are inoculated into BG-11 medium supplemented with inositol and cultured to obtain *Gynostemma pentaphyllum* spheroids. These spheroids are then inoculated into BG-11 medium supplemented with calcium adenosine diphosphate and cultured to obtain *Gynostemma pentaphyllum* powder. Finally, *Gynostemma pentaphyllum* polysaccharide is extracted using a water extraction and alcohol precipitation method. By improving the *Gynostemma pentaphyllum* cultivation process, high-quality *Gynostemma pentaphyllum* powder is obtained, thereby increasing the yield of *Gynostemma pentaphyllum* polysaccharide. The composition containing *Gynostemma pentaphyllum* polysaccharide extract is safe to use and exhibits excellent anti-wrinkle and firming effects.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an anti-wrinkle and firming composition, its preparation method, and its application. Background Technology

[0002] Modern lifestyles, characterized by fast pace and poor sleep, lead to neglected skincare. This, combined with ultraviolet radiation, electromagnetic radiation, unhealthy habits (such as smoking and drinking), excessive exercise, stress, and the overconsumption of highly processed foods and fats, contributes to significant skin problems such as sagging and aging. While numerous anti-wrinkle and firming cosmetics exist on the market, their effects are often unsatisfactory, failing to meet consumers' urgent needs for healthy and youthful skin.

[0003] Nostoc commune, a precious blue-green algae derived from nature, is renowned for its rich content of proteins, polysaccharides, and other active substances, exhibiting remarkable physiological activities such as antioxidation, antibacterial properties, anti-inflammation, immune enhancement, and tumor inhibition. In particular, Nostoc commune demonstrates outstanding performance in antioxidation and moisturizing, offering potential solutions for improving skin aging problems. For example, Chinese patent CN114224819A discloses a Nostoc commune homogenate extract, its preparation method, and its application. Applying this extract to cosmetics can achieve antioxidation, anti-glycation, and moisturizing effects. Chinese patent CN118490577A discloses a composite algae functional microalgae bead, its preparation method, and its application. This composite algae extract, combined with Nostoc commune extract and Spirulina extract—multi-functional skincare ingredients with repairing and anti-aging properties—has a synergistic effect of soothing, repairing, and anti-aging when used in skincare products.

[0004] However, the current application of Nostoc commune extract in cosmetics is limited by its extraction process, resulting in inconsistent extract quality and thus affecting its actual anti-wrinkle and firming effects. Traditional water extraction and alcohol precipitation methods, while able to obtain the effective components of Nostoc commune, suffer from low extraction rates, high impurity levels, and poor solubility, making it difficult to fully realize the potential of Nostoc commune. Chinese patent CN110713555A describes a process involving pre-pulverization, enzymatic hydrolysis, acid decolorization, centrifugal filtration, primary alcohol precipitation, redissolution, secondary alcohol precipitation, and drying of dried Nostoc commune to obtain Nostoc commune polysaccharides. However, the above extraction methods result in suboptimal quality of the Nostoc commune extract, thus affecting its efficacy in cosmetics. Therefore, conducting research and development on Nostoc commune and exploring new methods to improve the yield and quality of Nostoc commune extract can provide important theoretical basis for the widespread application of high-quality Nostoc commune in my country's cosmetic processing industry.

[0005] Based on this, the present invention improves the cultivation process of Nostoc commune to provide Nostoc commune with excellent quality, increases the content of active ingredients such as polysaccharides in Nostoc commune, and thus develops an anti-wrinkle and firming composition containing high-quality Nostoc commune extract. Summary of the Invention

[0006] The first objective of this invention is to provide an anti-wrinkle and firming composition that is safe to use and has excellent anti-wrinkle effects.

[0007] A second objective of this invention is to provide a method for preparing an anti-wrinkle and firming composition.

[0008] The third objective of this invention is to provide an application of an anti-wrinkle and firming composition, which has broad application prospects.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0010] An anti-wrinkle and firming composition comprises the following raw materials in the indicated weight percentages: acetyl hexapeptide-8 0.1-0.2%, *Gnaphalium affine* polysaccharide extract 0.3-0.5%, ethylhexylglycerin 0.051-0.069%, phenoxyethanol 0.53-0.55%, glycerin 25-35%, and the balance being water;

[0011] The preparation process of the Ge Xian Mi polysaccharide extract is as follows:

[0012] (1) Add inositol to BG-11 medium, and after sterilization, inoculate the *Gnaphalium affine* seed into the BG-11 medium for culture to obtain *Gnaphalium affine* spheroids;

[0013] (2) The spherical bodies of Ge Xian Mi from step (1) were inoculated into BG-11 medium supplemented with calcium adenosine diphosphate and cultured for a longer period of time to obtain Ge Xian Mi. After drying and pulverizing, Ge Xian Mi powder was obtained.

[0014] (3) Add the powder of Ge Xian Mi from step (2) to water, then add the compound enzyme to carry out the enzymatic hydrolysis reaction. After the enzymatic hydrolysis reaction is completed, perform enzyme inactivation treatment, filter the enzymatic hydrolysate, and obtain the supernatant. Add ethanol to the supernatant, let it stand, filter, and obtain the precipitate. Dry the precipitate to obtain Ge Xian Mi polysaccharide extract.

[0015] Preferably, the inositol content in the BG-11 culture medium in step (1) is 0.1-0.3 mg / L; the inoculum size of the *Smilax china* strain is 18-22 g / L; and the culture conditions are: temperature 15-20℃, time 20-25 days, and light intensity 100-120 μmol·m⁻¹. -2 ·s -1 .

[0016] Preferably, the content of calcium adenosine diphosphate in the BG-11 culture medium in step (2) is 1-5 mg / L; the inoculum size of the *Gnaphalium affine* spheroids is 18-22 g / L; and the culture conditions are: temperature 15-20℃, time 5-10 days, and light intensity 60-70 μmol·m⁻¹. -2 ·s -1 .

[0017] Preferably, in step (3), the ratio of the amount of *Ge Xian Mi* powder to the compound enzyme is 1 g: (4000-6000) U; the compound enzyme is composed of papain, cellulase, pectinase and neutral protease in a mass ratio of 1: (1-3): (1-2): (0.5-2.5); the temperature of the enzymatic hydrolysis reaction is 40-45℃, and the time is 7-8 h; the standing time is 4-6 h.

[0018] The preparation method of the above-mentioned anti-wrinkle and firming composition includes the following steps: mixing each raw material evenly according to the mass percentage ratio, thereby obtaining the final product.

[0019] The above-mentioned anti-wrinkle and firming composition is used in the preparation of cosmetics.

[0020] Preferably, the anti-wrinkle and firming composition comprises 1-5% by weight of the total weight of the cosmetic product.

[0021] Preferably, the cosmetic further includes the following raw materials in weight percentage: 4-6% moisturizer, 0.01-0.1% thickener, and the balance being water.

[0022] Preferably, the humectant is selected from sodium hyaluronate and 1,3-propanediol; the thickener is selected from carbomer, cellulose, and xanthan gum.

[0023] Compared with the prior art, the main advantages of the present invention are as follows:

[0024] This invention improves the cultivation process of *Gynostemma pentaphyllum*. Specifically, in the first cultivation stage, inositol is added to the culture medium. Inositol provides nutrients for the growth of *Gynostemma pentaphyllum*, promoting stable growth during cultivation. In the second cultivation stage, calcium adenosine diphosphate (ATP) is added to the culture medium. ATP is an important bioactive substance, thereby accelerating the growth and development of *Gynostemma pentaphyllum* and increasing the polysaccharide content. The performance of *Gynostemma pentaphyllum* and its polysaccharide extract obtained using the extraction method of this invention was tested. The results showed that the addition of inositol and ATP significantly improved the hardness and wet-dry weight ratio of *Gynostemma pentaphyllum*, giving it excellent quality. Furthermore, the purity of the *Gynostemma pentaphyllum* polysaccharide extract reached over 97.6%, and the yield exceeded 57.7%.

[0025] This invention combines extracted *Gnaphalium affine* polysaccharide with acetyl hexapeptide-8 and other components to form a composition, which is then applied to cosmetics. The effects of cosmetics containing this composition were tested, and the results showed that after using cosmetics containing this composition, the wrinkles and skin elasticity of the subjects' faces were significantly improved. This result also corroborates that the composition provided by this invention does indeed have a good anti-wrinkle and firming effect. Detailed Implementation

[0026] The technical solution of the present invention will be further described below with reference to specific embodiments. However, those skilled in the art should understand that the following embodiments are only for illustrating the present invention and should not be regarded as limiting the present invention. Specific conditions not specified in the embodiments are performed according to conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the reagents or instruments used are all conventional products obtained through commercial channels.

[0027] 1. Example

[0028] Example 1

[0029] An anti-wrinkle and firming composition comprises the following raw materials in weight percentages: 0.15% acetyl hexapeptide-8, 0.4% *Gynostemma pentaphyllum* polysaccharide extract, 0.056% ethylhexylglycerin, 0.54% phenoxyethanol, 27% glycerin, and the balance being water.

[0030] The preparation process of the Ge Xian Mi polysaccharide extract is as follows:

[0031] (1) Inositol was added to BG-11 medium at a concentration of 0.2 mg / L. After sterilization, *Ophiopogon japonicus* was inoculated into the medium at a concentration of 20 g / L and incubated at 18°C ​​and a light intensity of 110 μmol·m⁻¹. -2 ·s -1 Under a ventilated environment, the culture medium was changed every 10 days to obtain spherical gesso bodies.

[0032] (2) Add calcium adenosine diphosphate to BG-11 medium. The concentration of calcium adenosine diphosphate in BG-11 medium is 3 mg / L. Inoculate the spheroids of *Gnaphalium affine* from step (1) into the BG-11 medium supplemented with calcium adenosine diphosphate at an inoculation rate of 20 g / L. Incubate at 18°C ​​and a light intensity of 65 μmol·m⁻¹. -2 ·s -1 Under a well-ventilated environment, the rice was cultured for another 8 days to obtain *Ge Xian Mi* (a type of rice). The *Ge Xian Mi* was then dried and pulverized to obtain *Ge Xian Mi* powder.

[0033] (3) Following the ratio of *Ge Xian Mi* powder to compound enzyme of 1g:5000U, add the *Ge Xian Mi* powder from step (2) to 30 times its mass of water, then add the compound enzyme composed of papain, cellulase, pectinase, and neutral protease in a mass ratio of 1:2:1.5:1. Incubate at 42℃ for 7.5h. After the enzymatic hydrolysis, inactivate the enzyme at 95℃ for 30min, cool to room temperature, filter the hydrolysate, and obtain the supernatant. Add 3 times the volume of ethanol to the supernatant, let stand for 5h, filter, and obtain the precipitate. Vacuum dry the precipitate at 55℃ to obtain *Ge Xian Mi* polysaccharide extract.

[0034] The preparation method of the above-mentioned anti-wrinkle and firming composition is as follows:

[0035] Mix all ingredients thoroughly according to the above-mentioned mass percentage ratio to obtain the final product.

[0036] Example 2

[0037] An anti-wrinkle and firming composition comprises the following raw materials in weight percentages: 0.1% acetyl hexapeptide-8, 0.3% *Gynostemma pentaphyllum* polysaccharide extract, 0.051% ethylhexylglycerin, 0.53% phenoxyethanol, 25% glycerin, and the balance being water.

[0038] The preparation process of the Ge Xian Mi polysaccharide extract is as follows:

[0039] (1) Inositol was added to BG-11 medium at a concentration of 0.3 mg / L. After sterilization, *Ophiopogon japonicus* was inoculated into the medium at an inoculum concentration of 18 g / L. The medium was then incubated at 15°C under a light intensity of 100 μmol·m⁻¹. -2 ·s -1 Under a ventilated environment, the culture medium was changed every 20 days to obtain spherical gesso bodies.

[0040] (2) Add calcium adenosine diphosphate to BG-11 medium. The concentration of calcium adenosine diphosphate in BG-11 medium is 1 mg / L. Inoculate the spheroids of *Gnaphalium affine* from step (1) into the BG-11 medium supplemented with calcium adenosine diphosphate at an inoculation rate of 18 g / L. Incubate at 15°C and a light intensity of 60 μmol·m⁻¹. -2 ·s -1 Under a well-ventilated environment, the rice was cultured for another 5 days to obtain *Ge Xian Mi* (a type of rice). The *Ge Xian Mi* was then dried and pulverized to obtain *Ge Xian Mi* powder.

[0041] (3) Following the ratio of *Ge Xian Mi* powder to compound enzyme of 1g:4000U, add the *Ge Xian Mi* powder from step (2) to 30 times its mass of water, then add the compound enzyme composed of papain, cellulase, pectinase, and neutral protease in a mass ratio of 1:1:1:0.5. Incubate at 45℃ for 8 hours. After the enzymatic hydrolysis, inactivate the enzyme at 95℃ for 30 minutes, cool to room temperature, filter the hydrolysate, and obtain the supernatant. Add 3 times the volume of ethanol to the supernatant, let stand for 4 hours, filter, and obtain the precipitate. Vacuum dry the precipitate at 55℃ to obtain *Ge Xian Mi* polysaccharide extract.

[0042] The preparation method of the above-mentioned anti-wrinkle and firming composition is as follows:

[0043] Mix all ingredients thoroughly according to the above-mentioned mass percentage ratio to obtain the final product.

[0044] Example 3

[0045] An anti-wrinkle and firming composition comprises the following raw materials in weight percentages: 0.2% acetyl hexapeptide-8, 0.5% *Gynostemma pentaphyllum* polysaccharide extract, 0.069% ethylhexylglycerin, 0.55% phenoxyethanol, 35% glycerin, and the balance being water.

[0046] The preparation process of the Ge Xian Mi polysaccharide extract is as follows:

[0047] (1) Inositol was added to BG-11 medium at a concentration of 0.1 mg / L. After sterilization, *Ophiopogon japonicus* was inoculated into the medium at an inoculum concentration of 22 g / L. The medium was then incubated at 20°C under a light intensity of 120 μmol·m⁻¹. -2 ·s -1 Under a ventilated environment, the culture medium was changed every 10 days to obtain spherical gesso bodies.

[0048] (2) Add calcium adenosine diphosphate to BG-11 medium. The concentration of calcium adenosine diphosphate in BG-11 medium is 5 mg / L. Inoculate the spheroids of *Gnaphalium affine* from step (1) into BG-11 medium supplemented with calcium adenosine diphosphate at an inoculation rate of 22 g / L. Incubate at 20°C and a light intensity of 70 μmol·m⁻¹. -2 ·s -1 Under a well-ventilated environment, the rice was cultured for another 10 days to obtain *Ge Xian Mi* (a type of rice). The *Ge Xian Mi* was then dried and pulverized to obtain *Ge Xian Mi* powder.

[0049] (3) Following the ratio of *Ge Xian Mi* powder to compound enzyme of 1g:6000U, add the *Ge Xian Mi* powder from step (2) to 30 times its mass of water, then add the compound enzyme composed of papain, cellulase, pectinase, and neutral protease in a mass ratio of 1:3:2:2.5. Incubate at 40℃ for 7 hours. After the enzymatic hydrolysis, inactivate the enzyme at 95℃ for 30 minutes, cool to room temperature, filter the hydrolysate, and obtain the supernatant. Add 3 times the volume of ethanol to the supernatant, let stand for 6 hours, filter, and obtain the precipitate. Dry the precipitate under vacuum at 55℃ to obtain *Ge Xian Mi* polysaccharide extract.

[0050] The preparation method of the above-mentioned anti-wrinkle and firming composition is as follows:

[0051] Mix all ingredients thoroughly according to the above-mentioned mass percentage ratio to obtain the final product.

[0052] 2. Comparative Example

[0053] Comparative Example 1

[0054] The difference between Comparative Example 1 and Example 1 is that calcium phosphate was used instead of calcium adenosine diphosphate in the preparation of the Ge Xian Mi polysaccharide extract, while the rest remained the same as in Example 1.

[0055] Comparative Example 2

[0056] The difference between Comparative Example 2 and Example 1 is that adenosine was used instead of calcium adenosine diphosphate in the preparation of the Ge Xian Mi polysaccharide extract, while the rest remained the same as in Example 1.

[0057] Comparative Example 3

[0058] The difference between Comparative Example 3 and Example 1 is that in the preparation of Ge Xian Mi polysaccharide extract, inositol was not added to the culture medium in preparation step (1), while the rest was the same as in Example 1.

[0059] Comparative Example 4

[0060] The difference between Comparative Example 4 and Example 1 is that calcium adenosine diphosphate was not added to the culture medium in preparation step (2) of the preparation process of Ge Xian Mi polysaccharide extract, while the rest is the same as in Example 1.

[0061] Comparative Example 5

[0062] The difference between Comparative Example 5 and Example 1 is that in the preparation of Ge Xian Mi polysaccharide extract, inositol was not added to the culture medium in preparation step (1) and calcium adenosine diphosphate was not added to the culture medium in preparation step (2), while the rest was the same as in Example 1.

[0063] 3. Experimental Example

[0064] Experimental Example 1

[0065] The hardness and dry-to-wet weight ratio of the *Gynostemma pentaphyllum* obtained in step (2) during the preparation of the polysaccharide extracts of *Gynostemma pentaphyllum* obtained in Examples 1-3 and Comparative Examples 1-5 were tested.

[0066] The methods for testing hardness are as follows:

[0067] Ten Nostoc commune grains of the same diameter were randomly selected, and the moisture was absorbed with absorbent paper. The Nostoc commune grains were then placed on a glass plate, and the pressure required to crush the Nostoc commune grains was measured using a three-dimensional push-pull force gauge (SF-10N). The hardness data is the average value of the pressure required to crush the 10 Nostoc commune grains. The results are shown in Table 1.

[0068] The method for testing the wet-to-dry weight ratio is as follows:

[0069] Take 10g of Ge Xian Mi (a type of rice), absorb the surface moisture and weigh it, and record it as wet weight; after weighing, transfer it to an oven and dry it at 105℃ until constant weight, and record the dry weight value of each group. The formula for calculating the dry-wet weight ratio is: dry-wet weight ratio (%) = dry weight / wet weight × 100%, and the results are shown in Table 1.

[0070] Table 1

[0071] Grouping Dry to wet weight ratio (%) Hardness (N) Example 1 1.77±0.02 1.82±0.14 Example 2 1.71±0.03 1.79±0.30 Example 3 1.60±0.03 1.73±0.22 Comparative Example 1 1.39±0.05 1.50±0.51 Comparative Example 2 1.32±0.06 1.41±0.43 Comparative Example 3 0.78±0.07 0.94±0.45 Comparative Example 4 1.04±0.07 1.12±0.62 Comparative Example 5 0.56±0.05 0.75±0.37

[0072] As shown in Table 1, the dry-to-wet weight ratio and hardness of the Nostoc commune extracts in Examples 1-3 were higher than those in Comparative Examples 1-2. In Comparative Example 1, calcium phosphate was used instead of calcium adenosine diphosphate in the preparation of the Nostoc commune extract, while in Comparative Example 2, adenosine was used instead of calcium adenosine diphosphate in the preparation of the Nostoc commune extract.

[0073] The dry-to-wet weight ratio and hardness of the *Gnaphalium affine* from Examples 1-3 were significantly higher than those of Comparative Examples 3-5. Comparative Example 3 did not add inositol to the culture medium in preparation step (1), Comparative Example 4 did not add calcium adenosine diphosphate to the culture medium in preparation step (2), and Comparative Example 5 did not add inositol to either the culture medium in preparation step (1) or calcium adenosine diphosphate to the culture medium in preparation step (2). In summary, increasing the amount of inositol in the first culture stage and increasing the amount of calcium adenosine diphosphate in the second culture stage can significantly improve the quality of *Gnaphalium affine*.

[0074] Experiment Example 2

[0075] The polysaccharide extracts of *Gynostemma pentaphyllum* obtained in Examples 1-3 and Comparative Examples 1-5 were tested using the phenol-sulfuric acid method, and the purity of *Gynostemma pentaphyllum* polysaccharide was determined by high performance liquid chromatography. The results are shown in Table 2.

[0076] Table 2

[0077] Grouping Yield (%) of Nostoc commune polysaccharide extract Purity (%) of Ge Xian Mi polysaccharide extract Example 1 58.6 98.5 Example 2 58.1 97.9 Example 3 57.7 97.6 Comparative Example 1 51.5 97.0 Comparative Example 2 50.8 96.8 Comparative Example 3 47.2 96.1 Comparative Example 4 48.3 96.5 Comparative Example 5 45.1 95.4

[0078] As shown in Table 2, the purity of the *Ge Xian Mi* polysaccharide extract obtained by this invention is over 97.6%, and the yield is over 57.7%.

[0079] Compared with Examples 1-3, the yield of *Gynostemma pentaphyllum* polysaccharide extract obtained in Comparative Examples 1-5 was significantly reduced. This indicates that increasing the amount of inositol in the first culture stage and increasing the amount of calcium adenosine diphosphate in the second culture stage can significantly improve the quality of *Gynostemma pentaphyllum*, thereby increasing the yield of *Gynostemma pentaphyllum* polysaccharide extract.

[0080] 4. Application Examples

[0081] The compositions of Examples 1-3 are applied to the preparation of cosmetics. The following cosmetics are used as examples to illustrate the technical effects that the compositions of the present invention can achieve.

[0082] An essence comprising the following ingredients in weight percentages: 3% anti-wrinkle and firming composition, 5% 1,3-propanediol, 0.03% carbomer, and the balance being water.

[0083] Application Example 1

[0084] The safety of the serum composed of the compositions of Examples 1-3 was tested using a human skin patch test. The testing procedure is as follows:

[0085] Eighty healthy subjects aged 18-30 years were randomly selected and randomly divided into four groups, with a male-to-female ratio of 1:1 in each group.

[0086] Patch method: Using the closed patch test method, 0.025 mL of the essence and water (control group) composed of the compositions of Examples 1-3 were placed in the patch tester and applied externally to the flexor side of the subject's forearm with hypoallergenic adhesive tape. The test substance was removed after 24 hours. Skin reaction was observed at 0.5 hours, 24 hours and 48 hours after removal. The results were recorded according to the skin reaction grading standard in the "Cosmetic Safety Technical Specifications" (2015 edition). The results are shown in Table 3.

[0087] Table 3

[0088]

[0089] As shown in Table 3, the human skin patch experiment results indicated that 0 out of 20 participants experienced adverse skin reactions. These results demonstrate that the composition of the present invention is safe to use.

[0090] Application Example 2

[0091] Thirty healthy female subjects aged 35-50 with similar degrees of facial wrinkles were selected and randomly divided into three groups.

[0092] Before the experiment, the number and area of ​​wrinkles on the subjects' cheeks were counted using the Antera 3D skin analyzer, and the skin elasticity of the subjects' faces was tested using the MPA580 skin elasticity tester, and the results were recorded. The serum composed of the compositions from Examples 1-3 was applied evenly to the wrinkled areas of the subjects' cheeks (1-2 mL, a thin layer on the cheeks), twice daily, morning and evening, for 4 consecutive weeks. The number and area of ​​wrinkles on the subjects' cheeks were again counted using the Antera 3D skin analyzer, and the skin elasticity of the subjects' faces was tested again using the MPA580 skin elasticity tester, and the results were recorded. The degree of reduction in the number and area of ​​wrinkles on the cheeks of each group of subjects, as well as changes in skin elasticity, were analyzed. The experimental data are the average values ​​for each group, and the results are shown in Table 4.

[0093] Table 4

[0094]

[0095]

[0096] As shown in Table 4, after using the serum containing the compositions of Examples 1-3, the wrinkles and skin elasticity of the subjects' faces were significantly improved. That is, the composition can significantly improve facial wrinkles and skin elasticity.

[0097] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. Based on the present invention, some modifications or substitutions can be made, but these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of protection claimed by the present invention.

Claims

1. An anti-wrinkle and firming composition, characterized in that, It is composed of the following raw materials in weight percentage: acetyl hexapeptide-8 0.1-0.2%, *Gynostemma pentaphyllum* polysaccharide extract 0.3-0.5%, ethylhexylglycerin 0.051-0.069%, phenoxyethanol 0.53-0.55%, glycerin 25-35%, and the balance being water; The preparation process of the Ge Xian Mi polysaccharide extract is as follows: (1) Add inositol to BG-11 medium, and after sterilization, inoculate the *Gnaphalium affine* seed into the BG-11 medium for culture to obtain *Gnaphalium affine* spheroids; (2) The spherical bodies of Ge Xian Mi from step (1) were inoculated into BG-11 medium supplemented with calcium adenosine diphosphate and cultured for a longer period of time to obtain Ge Xian Mi. After drying and pulverizing, Ge Xian Mi powder was obtained. (3) Add the powder of Nostoc commune from step (2) to water, then add the compound enzyme to carry out the enzymatic hydrolysis reaction. After the enzymatic hydrolysis reaction is completed, perform enzyme inactivation treatment, filter the enzymatic hydrolysate, and obtain the supernatant; add ethanol to the supernatant, let it stand, filter, and obtain the precipitate; dry the precipitate to obtain Nostoc commune polysaccharide extract. The inositol content in the BG-11 culture medium mentioned in step (1) is 0.1-0.3 mg / L; The content of calcium adenosine diphosphate in the BG-11 medium mentioned in step (2) is 1-5 mg / L; The complex enzyme described in step (3) is composed of papain, cellulase, pectinase and neutral protease in a mass ratio of 1:(1-3):(1-2):(0.5-2.5).

2. The anti-wrinkle and firming composition according to claim 1, characterized in that, The inoculum size of *Spartina alterniflora* in step (1) is 18-22 g / L; the culture conditions are: temperature 15-20℃, time 20-25 days, and light intensity 100-120 µmol·m⁻¹. -2 ·s -1 .

3. The anti-wrinkle and firming composition as described in claim 1, characterized in that, The inoculum size of *Gnaphalium affine* spheroids in step (2) is 18-22 g / L; the culture conditions are: temperature 15-20℃, time 5-10 days, and light intensity 60-70 µmol·m⁻¹. -2 ·s -1 .

4. The anti-wrinkle and firming composition according to claim 1, characterized in that, In step (3), the ratio of the amount of Ge Xian Mi powder to the compound enzyme is 1g:(4000-6000)U; the temperature of the enzymatic hydrolysis reaction is 40-45℃ and the time is 7-8h; the standing time is 4-6h.

5. A method for preparing the anti-wrinkle and firming composition according to any one of claims 1-4, characterized in that, Includes the following steps: Mix all raw materials evenly according to the stated mass percentage ratio to obtain the final product.

6. The application of the anti-wrinkle and firming composition according to any one of claims 1-4, characterized in that, Applications in the preparation of cosmetics.

7. The application of the anti-wrinkle and firming composition according to claim 6, characterized in that, The anti-wrinkle and firming composition comprises 1-5% by weight of the total mass of the cosmetic product.

8. The application of the anti-wrinkle and firming composition according to claim 6, characterized in that, The cosmetic also includes the following ingredients by weight percentage: 4-6% moisturizer, 0.01-0.1% thickener, and the remainder is water.

9. The application of the anti-wrinkle and firming composition according to claim 8, characterized in that, The humectant is selected from sodium hyaluronate and 1,3-propanediol; the thickener is selected from carbomer, cellulose, and xanthan gum.

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