Multifunctional skin-care plant composition containing exocarpium citri grandis and application of multifunctional skin-care plant composition

Abstract

The invention provides a multifunctional skin care plant composition containing exocarpium citri grandis and application, and belongs to the field of skin care products. The composition comprises a pomelo fruit extract, a butterfly bean flower extract, a pollen typhae extract and a cauliflower extract. The composition disclosed by the invention is natural in source, so that the problem of monomer residue possibly caused by chemical synthesis is avoided, and the green and environment-friendly characteristics of the product are also ensured. Compared with skin-brightening antioxidant components which depend on imported raw materials, are high in cost and have doubt safety in the market, the skin-brightening antioxidant composition is beneficial to reducing psychological burdens of consumers. The composition is a water-soluble flavonoid compound in compatibility, is wide in application range and simple in compatibility, can be used in various dosage forms such as water cream essence and the like, and does not reduce the skin brightening and oxidation resisting effects of cream oil-containing products when being used in the cream oil-containing products.

Description

Technical Field

[0001] The present invention belongs to the field of skin care products, and particularly relates to a multi-functional skin care plant composition containing tangerine peel and its application. Background Art

[0002] In the cosmetics market, the demand for products with skin brightening, antioxidant and anti-wrinkle effects is increasing day by day. However, natural cosmetic ingredients and related technologies with such effects are still relatively scarce. Widely used cosmetic raw materials in the market, such as derivatives of niacinamide, arbutin, vitamin C, etc., are mostly chemically synthesized raw materials, and there is a possibility of monomer residue in actual application, which poses a high risk of irritation to the skin. In recent years, most cosmetic ingredients with skin brightening and antioxidant effects on the market have been controversial due to safety issues. Consumers have doubts about the safety of some raw materials, which leads to poor user experience, affects market trust, and reduces the attractiveness of products.

[0003] Therefore, skin brightening ingredients of natural origin are very popular because of their green, safe and low-irritation properties. There are also some patents that focus on skin brightening ingredients of natural origin:

[0004] ①. Chinese Patent Publication No.: CN113476674A, titled: "A Composition with Skin Brightening, Spot Fading and Antioxidant Effects, Its Preparation Method and Application", uses Mirabilis jalapa cell actives and Rosa × alba cell actives. Although it has unique effects, the preparation process of cell actives is complex and the cost is high, which is not conducive to large-scale promotion of products.

[0005] ②. Chinese Patent Publication No.: CN114073744A, titled "A Method for Preparing an Antioxidant and Skin Brightening Composition and a Skin Care Product Containing the Composition", the composition contains various ingredients such as Narcissus tazetta bulb extract. Although the antioxidant and skin brightening effects are good, the sources of some raw materials are relatively limited. For example, the extract of Xylaria nigripes may face supply problems in large-scale production. At the same time, the patent does not elaborate on the synergistic mechanism of each ingredient in detail, which is not conducive to further optimizing the formula, and does not mention the applicability to sensitive skin, which may limit the product audience.

[0006] ③. Chinese Patent Publication No.: CN202410167671.0, titled "Use of Loquat Seed Extract in the Preparation of Skin Antioxidant and / or Skin Brightening Products" shows that loquat seed extract has good effects in skin brightening and antioxidant. However, it only focuses on loquat seed extract, and it is difficult to play a role from multiple pathways like a multi-component synergistic formula when dealing with complex skin aging and pigmentation problems. In terms of the skin brightening mechanism, it mainly achieves skin brightening and antioxidant effects through conventional pathways such as inhibiting tyrosinase activity and scavenging free radicals, and the pathway is relatively single.

[0007] Therefore, there is an urgent need in the market for a skin brightening ingredient of natural origin, which has strong antioxidant properties and can carry out antioxidant activities through multiple pathways, thereby achieving multi-pathway antioxidant effects. SUMMARY OF THE INVENTION

[0008] The object of the present invention is to address the aforementioned problems and provide a multi-functional skin care plant composition containing Exocarpium Citri Grandis and its applications.

[0009] To achieve the above object, the present invention provides a multi-functional skin care plant composition, which comprises the following components in parts by mass:

[0010] Pomelo fruit extract 30 - 50 parts

[0011] Butterfly pea flower extract 20 - 40 parts

[0012] Typha angustifolia pollen extract 10 - 30 parts

[0013] Cauliflower extract 1 - 20 parts.

[0014] Preferably, it comprises the following components in parts by mass:

[0015] Pomelo fruit extract 40 - 50 parts

[0016] Butterfly pea flower extract 30 - 40 parts

[0017] Typha angustifolia pollen extract 15 - 30 parts

[0018] Cauliflower extract 1 - 10 parts.

[0019] More preferably, it comprises the following components in parts by mass:

[0020] Pomelo fruit extract 30 - 40 parts

[0021] Butterfly pea flower extract 20 - 30 parts

[0022] Typha angustifolia pollen extract 10 - 25 parts

[0023] Cauliflower extract 10 - 20 parts.

[0024] Even more preferably, it comprises the following components in parts by mass:

[0025]

[0026] Preferably, the pomelo fruit of the pomelo fruit extract is CITRUS GRANDIS.

[0027] Preferably, the butterfly pea flower of the butterfly pea flower extract is CLITORIA TERNATEA.

[0028] Preferably, the cattail pollen extract is from TYPHA ANGUSTIFOLIA.

[0029] Preferably, the cauliflower extract is from BRASSICA OLERACEA BOTRYTIS.

[0030] The present invention also provides the application of the multi-functional skin care plant composition in the preparation of topical skin agents; the multi-functional skin care plant composition has an antioxidant effect on the skin.

[0031] The composition provided by the present invention is composed of pomelo fruit extract, butterfly pea flower extract, cattail pollen extract, and cauliflower extract. The formed composition has been experimentally verified to have the effects of brightening the skin and antioxidant. And the experiments show that, compared with the composition lacking some components, the composition provided by the present invention has more significant effects, and has more advantages in terms of both skin brightening and antioxidant, indicating that the components in this composition play a good synergistic effect, promoting and coordinating with each other and are indispensable.

[0032] The pomelo fruit extract (i.e., excipient, also known as Citrus grandis 'Tomentosa') of the present invention is one of the core components of this combination.

[0033] "Excipient" comes from Huazhou City, Guangdong Province, and is a local special plant with extremely high edible and pharmacological values; it mainly contains flavonoid compounds such as nobiletin, hesperidin, neohesperidin, etc., and has good antioxidant, anti-inflammatory and antibacterial effects. The pomelo fruit extract can inhibit the mitogen-activated protein kinase (MAPK) signaling pathway and reduce the expression of microphthalmia-associated transcription factor (MITF). MITF is a key transcription factor that regulates the differentiation of melanocytes and melanin synthesis. The decrease in its expression will lead to a decrease in the expression of melanin synthesis-related enzymes such as tyrosinase, thereby inhibiting melanin synthesis. In addition, the pomelo fruit extract can activate antioxidant enzymes in skin cells, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), etc. These enzymes can catalyze the oxidation-reduction reaction in the body, decompose harmful peroxides into harmless substances, enhance the skin's own antioxidant defense ability, and maintain the healthy state of skin cells. The polysaccharide substances in the pomelo fruit extract form a skin barrier and increase the water content of the stratum corneum. Clinical tests found that the skin moisture increased by 28% after 4 weeks under the action of the pomelo fruit extract.

[0034] The extraction process of the present invention includes the steps of: smashing the fruits of Exocarpium Citri Grandis (Tomentosa), evacuating the air, extracting and concentrating with alcohol, sterilizing, and storing frozen, purposefully avoiding the destruction of thermosensitive components in the raw materials by high temperature and retaining the active components to the greatest extent; alcohol extraction and concentration can efficiently extract the target components and improve the extraction rate; sterilization treatment can ensure the purity and sterility of the extract and guarantee the quality and safety; frozen storage helps to maintain the stability and biological activity of the extract.

[0035] The main active ingredient of the butterfly pea flower extract of the present invention is procyanidin, which can help promote the elasticity and collagen of the skin. Procyanidin has multiple phenolic hydroxyl structures, can chelate with metal ions to form stable complexes, inhibit the generation of free radicals catalyzed by metal ions, and thus indirectly inhibit the activation of tyrosinase, achieving the effect of brightening the skin. The butterfly pea flower extract of the present invention is produced in Hainan, China, contains various vitamins and minerals, and can play a role in nourishing the skin and improving the skin texture in cosmetics, making the skin smoother and more delicate.

[0036] The Typha angustata Bory et Chaub. pollen extract of the present invention contains sterol components. ① Sterols can capture free radicals on the skin surface, such as hydroxyl free radicals and superoxide anion free radicals, through their own structural characteristics, and prevent the oxidative attack of free radicals on skin cells, thereby protecting the cell components from damage. ② Sterols can regulate the fluidity and stability of the skin cell membrane, reduce the damage of the cell membrane caused by oxidative stress, and enable the cells to better exert their own antioxidant defense functions. ③ Sterols may also participate in regulating the antioxidant enzyme system in skin cells, enhancing the activities of superoxide dismutase, glutathione peroxidase, etc., and improving the overall antioxidant capacity of the skin. ④ In terms of skin brightening, sterols can inhibit the activity of tyrosinase, which is a key enzyme for melanin synthesis. By inhibiting its activity, the generation of melanin can be reduced, thus achieving the effect of brightening the skin. ⑤ Sterols can also promote skin metabolism, accelerate the metabolism and excretion of melanin, make the skin brighter and whiter, and play its role in skin brightening and antioxidant in cosmetics from multiple aspects.

[0037] The main active ingredients of the broccoli extract of the present invention are vitamin C, vitamin E, flavonoids, and various minerals. ① Vitamin C inhibits the activity of tyrosinase, prevents tyrosine from being converted into dopaquinone, and further reduces the synthesis of melanin; it can reduce the already formed melanin to colorless reduced melanin, promote the decomposition and metabolism of melanin, achieve the effect of brightening the skin, and make the skin brighter and more shiny. ② Vitamin C and vitamin E act synergistically to regenerate other antioxidants and enhance the overall antioxidant effect.

[0038] This product is a water-soluble substance with a wide range of applications and simple compatibility in cosmetic formulations. It can be used in various dosage forms such as water, milk, cream, essence, etc., and its brightening and antioxidant effects are not reduced when used in oil-phase products such as creams. (Some water-soluble brightening and antioxidant raw materials on the market are easily oxidized by air, restricting the scope of product use.)

[0039] The present invention has the following advantages:

[0040] (1) The compositions of the present invention are all derived from natural sources, avoiding the problem of monomer residues that may be brought about by chemical synthesis, and ensuring the green and environmental protection characteristics of the products. Compared with the brightening and antioxidant ingredients on the market that rely on imported raw materials, have high costs, and have questionable safety, the present invention uses natural raw materials, has a simple preparation method, and low costs, is suitable for large-scale production and industrial promotion, and helps to reduce the economic burden on consumers.

[0041] (2) The present invention combines extracts of pomelo fruit, butterfly pea flower, cattail pollen, and broccoli; the complex of the present invention has undergone a zebrafish efficacy test, and the results show that this ingredient has brightening and antioxidant effects.

[0042] (3) When used in formulations, it has good compatibility and stability. In terms of compatibility, the product is a water-soluble flavonoid compound, can be made into transparent products, has a wide range of applications, simple compatibility, and can be used in various dosage forms such as water, milk, cream, and essence. Its brightening and antioxidant effects are not reduced when used in oil-phase products such as creams. In terms of stability, the composition remains its original properties after being simulated in a cosmetic formulation under environments such as 50°C high temperature, -10°C low temperature, and ultraviolet irradiation for 3 months, and the active ingredients do not decompose or become ineffective, effectively ensuring the product quality. Description of the Drawings

[0043] Figure 1 This is the normal control group in the differential analysis of the melanin signal intensity in the heads of zebrafish in the normal control group and the sample group.

[0044] Figure 2 This is Example 1 (1%) in the differential analysis of the melanin signal intensity in the heads of zebrafish in the normal control group and the sample group.

[0045] Figure 3 The zebrafish antioxidant efficacy test is a column chart of the fluorescence intensity of the yolk sac. Detailed Embodiments

[0046] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0047] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of the said features. In the description of the present invention, "a plurality of" means two or more, unless otherwise specifically defined.

[0048] In the description of the present invention, the term "for example" is used to mean "serving as an example, illustration, or explanation". Any embodiment described as "for example" in the present invention is not necessarily construed as being more preferred or advantageous than other embodiments. The following description is given to enable any person skilled in the art to implement and use the present invention. In the following description, details are set forth for purposes of explanation. It should be understood that those of ordinary skill in the art can recognize that the present invention can be implemented without using these specific details. In other instances, well-known structures and processes are not elaborated in detail to avoid obscuring the description of the present invention with unnecessary details. Therefore, the present invention is not intended to be limited to the embodiments shown, but is consistent with the broadest scope that conforms to the principles and features disclosed in the present invention.

[0049] Unless otherwise specified, the experimental methods used in the specific embodiments are all conventional methods, and the materials, reagents, etc. used, unless otherwise specified, can all be obtained from commercial channels.

[0050] In the present invention, unless otherwise specified, "%" represents mass percentage; the raw materials, reagents, etc. used are all conventional commercially available products.

[0051] In the present invention, the water used is preferably pure water (RO water).

[0052] In the present invention, the pomelo fruit extract is the extract of Exocarpium Grandis; the INCI name is extract of Citrus grandis.

[0053] In the present invention, the butterfly pea flower extract has the INCI name of extract of Clitoria ternatea flower.

[0054] In the present invention, the cattail pollen extract has the INCI name of extract of Typha angustifolia.

[0055] In the present invention, the broccoli extract has the INCI name of extract of Brassica oleracea botrytis.

[0056] In the present invention, the lemon extract has the INCI name of extract of Citrus limon.

[0057] In the present invention, the INCI name of the wisteria flower extract is WISTERIA SINENSIS flower extract.

[0058] In the present invention, the INCI name of the cattail extract is TYPHA ORIENTALIS extract.

[0059] In the present invention, the INCI name of the radish extract is RAPHANUS SATIVUS root extract.

[0060] For the preparation method of the plant extract, weigh each raw material according to the parts by mass in Table 1 below. Take a stirring pot as the container, add an appropriate amount of water, turn on the stirring function and heat up to 60°C, and keep the temperature in the pot at 60 ± 2.5°C; until all components are dissolved and mixed into a homogeneous state; then stop heating and cool to room temperature.

[0061] Table 1 Preparation ratio of the composition

[0062] Component Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Pomelo Fruit Extract 30 40 50 0 40 Clitoria Ternatea Extract 40 30 20 40 0 Typha Angustata Bory Et Chaub Extract 10 20 29 40 35 Cauliflower Extract 20 10 1 20 25 Lemon Extract 0 0 0 0 0 Wisteria Flower Extract 0 0 0 0 0 Typha Extract 0 0 0 0 0 Radish Extract 0 0 0 0 0 Water 900 900 900 900 900

[0063] Table 1 (continued) Preparation ratio of the composition

[0064] Component Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Pomelo Fruit Extract 50 40 0 40 40 40 Clitoria Ternatea Extract 40 30 30 0 30 30 Typha Angustata Bory Et Chaub Extract 0 30 20 20 0 20 Cauliflower Extract 10 0 10 10 10 0 Lemon Extract 0 0 40 0 0 0 Wisteria Flower Extract 0 0 0 30 0 0 Typha Extract 0 0 0 0 20 0 Radish Extract 0 0 0 0 0 10 Water 900 900 900 900 900 900

[0065] The above-prepared composition was subjected to experimental tests as follows:

[0066] Zebrafish whitening efficacy test: Take 1% of the diluted solutions of the examples and the comparative examples for the zebrafish whitening efficacy test. Randomly select zebrafish with basically the same growth and development into a 6-well plate, 15 tails per well; administer the sample by water solution, and at the same time set up a normal control group, with a volume of 3 mL per well; incubate in the dark at 28.5°C for 45 - 48 h; randomly select 10 zebrafish from each experimental group and place them under a stereomicroscope for photographing, analyze and collect data using advanced image processing software, analyze the melanin signal intensity (S) of the zebrafish head, and calculate the skin brightening efficacy of the sample according to the formula to determine whether it has skin brightening efficacy.

[0067]

[0068] The results of Example 1 are shown in Table 2 and Figure 1 、 Figure 2 as follows: After detection, the melanin signal intensity of the zebrafish head in the 1% sample group (Example 1) was significantly reduced compared with the normal control group, revealing that the sample has skin brightening efficacy.

[0069] Table 2 Zebrafish whitening efficacy test

[0070]

[0071] Enlarged tests were conducted on other examples and comparative examples, with the same test method; the results are shown in Table 3 below.

[0072] Table 3 Zebrafish Whitening Efficacy Test (Enlarged Test, Dosage 1%)

[0073]

[0074]

[0075] Zebrafish Antioxidant Efficacy Test: Take 0.5% diluted solutions of the examples and comparative examples for zebrafish antioxidant efficacy test. Randomly select zebrafish into 6-well plates, with 15 tails in each well; add menadione to establish a zebrafish oxidative stress model; administer the samples by water solution, and at the same time set up a normal control group and a model control group, with a volume of 3 mL in each well; incubate in the dark at 28.5 °C for 20 h; stain the zebrafish with a specific fluorescent reagent staining solution; randomly select 10 zebrafish from each experimental group and place them under a fluorescence microscope for photographing, analyze and collect data using advanced image processing software, analyze the fluorescence intensity (S) of the zebrafish yolk sac, and calculate the antioxidant efficacy value of the sample according to the formula to determine whether it has antioxidant efficacy.

[0076]

[0077] The results of Example 1 are shown in Table 4 and Figure 3 : After detection, the fluorescence intensity of the yolk sac of zebrafish in the 0.5% sample group (Example 1) was significantly reduced compared with the model control group, revealing that the sample has antioxidant efficacy.

[0078] Table 4 Antioxidant Efficacy Test

[0079]

[0080] Enlarged tests were conducted on other examples and comparative examples, with the same test method; the results are shown in Table 5 below.

[0081] Table 5 Antioxidant Efficacy Test (Enlarged Test, Dosage 0.5%)

[0082]

[0083]

[0084] Comparative Examples 1, 2, 3, and 4 are default tests compared with Example 1; Comparative Examples 5, 6, 7, and 8 are component substitution tests compared with Example 2; according to the foregoing experimental results, it can be seen that on the premise of the same content of efficacy components, the absence or substitution of any component in the present invention will cause a certain decrease in its skin brightening or antioxidant ability; it shows that each component in the composition of the present invention plays a good synergistic effect, promoting and coordinating with each other and none of them can be missing. In addition, compared with some compositions with improper ratios, the composition provided by the present invention also has better skin brightening and antioxidant effects, indicating that the ratio of the composition has a significant impact on the effect, and the ratio of the composition provided by the present invention is a verified and preferred ratio.

[0085] Specifically, the pomelo fruit extract mainly contains flavonoids, the butterfly pea flower extract mainly contains proanthocyanidins, the cattail pollen extract mainly contains sterols, and the broccoli extract mainly contains vitamin C and vitamin E, each playing a unique effect. In the application scenario of cosmetics, the mechanism of action complements each other, synergistically interrupting the free radical chain reaction, preventing the generation of free radicals such as the Fenton reaction, chelating metal ions, inhibiting the generation of free radicals, reducing cell membrane damage caused by oxidative stress, reducing the formed melanin to colorless reduced melanin, and promoting the decomposition and metabolism of melanin, etc., to achieve the purpose of synergy and significantly optimize the skin brightening and antioxidant effects.

[0086] The above detailed description is a specific description of one feasible embodiment of the present invention, and this embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or change that does not depart from the present invention shall be included within the scope of the technical solution of the present invention.

Claims

1. A multi-functional skin care plant composition, characterized in that, Comprising the following components in parts by mass: 30 - 50 parts of pomelo fruit extract 20 - 40 parts of butterfly pea flower extract 10 - 30 parts of cattail pollen extract 1 - 20 parts of cauliflower extract.

2. The multi-functional skin care plant composition according to claim 1, characterized in that, Comprising the following components in parts by mass: 40 - 50 parts of pomelo fruit extract 30 - 40 parts of butterfly pea flower extract 15 - 30 parts of cattail pollen extract 1 - 10 parts of cauliflower extract.

3. The multi-functional skin care plant composition according to claim 1, characterized in that, Comprising the following components in parts by mass: 30 - 40 parts of pomelo fruit extract 20 - 30 parts of butterfly pea flower extract 10 - 25 parts of cattail pollen extract 10 - 20 parts of cauliflower extract.

4. The multi-functional skin care plant composition according to claim 1, characterized in that, Comprising the following components in parts by mass: 40 parts of pomelo fruit extract 30 parts of butterfly pea flower extract 20 parts of cattail pollen extract 10 parts of cauliflower extract.

5. The multi-functional skin care plant composition according to claim 1, characterized in that, The pomelo fruit of the pomelo fruit extract is CITRUS GRANDIS.

6. The multi-functional skin care plant composition according to claim 1, characterized in that The butterfly pea flower of the butterfly pea flower extract is CLITORIA TERNATEA.

7. The multi-functional skin care plant composition according to claim 1, characterized in that, The cattail pollen of the cattail pollen extract is TYPHA ANGUSTIFOLIA.

8. The multi-functional skin care plant composition according to claim 1, characterized in that, The cauliflower of the cauliflower extract is BRASSICA OLERACEA BOTRYTIS.

9. Use of the multi - efficacy skin - care plant composition according to any one of claims 1 - 8 in the preparation of a topical skin agent.

10. Use according to claim 9 in the preparation of a topical skin preparation, characterized in that The multi - efficacy skin - care plant composition has a skin antioxidant effect.

Images