Traditional Chinese medicine composition for repairing muscle, tendon and skeleton injury and preparation method of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition for repairing muscle, tendon and skeleton injuries and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition for repairing muscle, tendon and skeleton injuries is prepared from the following traditional Chinese medicines in parts by weight: 5-10 parts of garden balsam stem, 1-3 parts of raw semen strychni, 3-5 parts of radix aconiti agrestis, 5-10 parts of sambucus chinensis, 5-10 parts of paris polyphylla, 1-3 parts of radix zanthoxyli, 5-10 parts of angelica sinensis, 5-10 parts of safflower, 3-5 parts of notopterygium root, 3-5 parts of willow bark and 5-10 parts of peach kernel. , 3-5 parts of ligusticum wallichii, 5-10 parts of eucommia ulmoides, 5-10 parts of rhizoma cibotii, 3-5 parts of dipsacus root and 5-10 parts of liquorice. The traditional Chinese medicine composition disclosed by the invention can play a role in continuously treating and repairing diseases, assists muscles, tendons and bones to gradually repair and recover, and prevents relapse.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries and a preparation method thereof. Background Art

[0002] Muscle, tendon and bone injuries are caused by long-term bad living or working habits in daily life, or accidental external force collisions, resulting in muscle, tendon or joint strain, bone displacement and dislocation, nerve compression, various inflammations, pain, swelling, fever, tenderness and functional impairment; when the inflammation affects the joint, tissue fluid can accumulate in the joint, causing swelling, reduced range of motion, nerve compression, unbearable pain and difficult movement.

[0003] Currently, various commonly used external traditional Chinese medicine repair liquids for relieving pain and inflammation of bone, muscle and tendon injuries can only penetrate into the skin surface or inner layer, and relieve symptoms by anesthetizing nerves in the short term for analgesia and anti-inflammation, but cannot penetrate deep into the muscle or bone to repair the affected area. The pain will recur after stopping the medicine, and effective repair cannot be achieved. This causes patients to be unable to relieve the pain and has a continuous impact on their daily work and life.

[0004] In view of this, the applicant proposes a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries. Summary of the Invention

[0005] The technical problem to be solved by the present invention is, in view of the deficiencies of the prior art, to provide a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries, which can penetrate layer by layer into the muscle and bone, and in addition to analgesia and anti-inflammation, also has a repair effect on muscle, tendon and bone injuries.

[0006] To solve the above technical problem, the technical solution adopted by the present invention is: a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries, which is made of the following traditional Chinese medicines in parts by weight: 5-10 parts of Speranskia tuberculata, 1-3 parts of raw Strychnos nux-vomica, 3-5 parts of Kusnezoff Monkshood Root, 5-10 parts of Saururus chinensis, 5-10 parts of Paris polyphylla, 1-3 parts of Zanthoxylum nitidum, 5-10 parts of Angelica sinensis, 5-10 parts of Carthamus tinctorius, 3-5 parts of Notopterygium incisum, 3-5 parts of Willow bark, 5-10 parts of Prunus persica, 3-5 parts of Ligusticum chuanxiong, 5-10 parts of Eucommia ulmoides, 5-10 parts of Cibotium barometz, 3-5 parts of Dipsacus asperoides, 5-10 parts of Licorice root.

[0007] Speranskia tuberculata: The whole plant of Speranskia tuberculata (Bunge) Baill. of the genus Speranskia in the Euphorbiaceae family. Wash, dry in the sun, and cut into pieces for use. It belongs to the liver and kidney meridians. Pungent, bitter, warm. Slightly toxic. Expelling wind and removing dampness, detoxifying and relieving pain.

[0008] Unprocessed semen strychni: The dried and mature seeds of Strychnos nuxvomica L., a plant of the family Loganiaceae. Remove impurities from unprocessed semen strychni. It belongs to the liver and spleen meridians. Bitter in taste, warm in nature; extremely poisonous. It dredges collaterals to relieve pain and dissipates binds to reduce swelling.

[0009] Radix aconiti kusnezoffii: The dried tuberous root of Aconitum kusnezoffii Reichb., a plant of the family Ranunculaceae. It belongs to the heart, liver, kidney, and spleen meridians. Pungent and bitter in taste, hot in nature; extremely poisonous. It dispels wind and dampness, and warms the channels to relieve pain.

[0010] Caulis sambuci chinensis: The stem and branches of Sambucus williamsii Hance, a plant of the family Caprifoliaceae. Nature and flavor: sweet, bitter, and neutral. It dispels wind, promotes diuresis, activates blood circulation, and relieves pain.

[0011] Rhizoma paridis: The rhizome of Paris polyphylla Smith var. chinensis (Franch.) Hara or Paris polyphylla Smith of the genus Paris in the family Liliaceae, used as medicine. Bitter in taste, cold in nature. Slightly poisonous. It clears heat and detoxifies, and reduces swelling and relieves pain.

[0012] Radix zanthoxyli nitidi: The dried root of Zanthoxylum nitidum (Roxb.) DC., a plant of the family Rutaceae. It belongs to the liver and stomach meridians. Nature and flavor: bitter and pungent, neutral; slightly poisonous. It promotes qi flow to relieve pain, activates blood circulation to dissipate stasis, and dispels wind and dredges collaterals.

[0013] Radix angelicae sinensis: The dried root of Angelica sinensis (Oliv.) Diels, a plant of the family Umbelliferae. Remove impurities, wash, moisten until soft, cut into thin slices, and dry in the sun or by low-temperature drying. It belongs to the liver, heart, and spleen meridians. Nature and flavor: sweet and pungent, warm. Functions and indications: Tonifies blood and promotes blood circulation, regulates menstruation and relieves pain, moistens the intestines and promotes defecation.

[0014] Flos carthami: The dried flower of Carthamus tinctorius L., a plant of the family Compositae. It belongs to the heart and liver meridians. Nature and flavor: pungent, warm. It promotes blood circulation to dredge the channels, dispels stasis and relieves pain.

[0015] Rhizoma et radix notopterygii: The dried rhizome and root of Notopterygium incisum Ting ex H. T. Chang or Notopterygium forbesii Boiss., plants of the family Umbelliferae. Remove impurities, wash, moisten until soft, cut into thick slices, and dry in the sun. It belongs to the bladder and kidney meridians. Nature and flavor: pungent and bitter, warm. It dispels cold, expels wind, removes dampness, and relieves pain.

[0016] Cortex salicis babylonicae: The branches or the phloem of the roots of Salix babylonica L., a plant of the family Salicaceae. Remove the cork and xylem, and use the phloem. "Tang Materia Medica": "The branch bark is bitter in taste, cold in nature, and non-toxic." It dispels wind and promotes diuresis, reduces swelling and relieves pain.

[0017] Peach Kernel: It is the dried ripe seed of Prunus persica (L.) Batsch or Prunus davidiana (Carr.) Franch. of the Rosaceae family. Remove impurities. Pound it before use. It belongs to the heart, liver, and large intestine meridians. Properties and flavors: Bitter, sweet, and neutral. It promotes blood circulation to remove stasis and moistens the intestines to relieve constipation.

[0018] Chuanxiong Rhizome: It is the dried rhizome of Ligusticum chuanxiong Hort. (or Ligusticum wallichii Franch.) of the Umbelliferae family. Remove impurities, separate them by size, soak slightly, wash, moisten thoroughly, cut into thin slices, and dry. It belongs to the liver, gallbladder, and pericardium meridians. Properties and flavors: Pungent, warm. Functions and indications: Promote blood circulation and qi movement, dispel wind and relieve pain.

[0019] Eucommia Bark: It is the dried bark of Eucommia ulmoides Oliv. of the Eucommiaceae family. Scrape off the remaining rough bark, wash, cut into pieces or filaments, and dry. It belongs to the liver and kidney meridians. Properties and flavors: Sweet, warm. Functions and indications: Tonify the liver and kidney, strengthen tendons and bones, and prevent miscarriage.

[0020] Golden Hair Fern Rhizome: It is the dried rhizome of Cibotium barometz (L.) J.Sm. of the Dryopteridaceae family. Remove impurities; for those not sliced, wash, moisten thoroughly, cut into thick slices, and dry. It belongs to the liver and kidney meridians. Properties and flavors: Bitter, sweet, warm. Functions and indications: Tonify the liver and kidney, strengthen the lumbar spine, and dispel wind-dampness.

[0021] Dipsacus Root: It is the root of Dipsacus asperoides C.Y.Cheng et T.M.Ai. of the Dipsacaceae family. Properties and flavors: Slightly warm in nature, bitter and pungent. It belongs to the liver and kidney meridians. Functions and indications: Tonify the liver and kidney, strengthen tendons and bones, repair fractures, and stop metrorrhagia and metrostaxis.

[0022] Licorice Root: It is the dried root of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., or Glycyrrhiza glabra L. of the Leguminosae family. Remove impurities, wash, moisten thoroughly, cut into thick slices, and dry. It belongs to the heart, lung, spleen, and stomach meridians. Properties and flavors: Sweet, neutral. Tonify the spleen and replenish qi, clear heat and detoxify, resolve phlegm and relieve cough, relieve spasm and pain, and harmonize various herbs.

[0023] Optionally, the traditional Chinese medicine composition for repairing muscle, tendon, and bone injuries is made from the following traditional Chinese medicines in parts by weight: 7 parts of Speranskia tuberculata, 2 parts of Strychnos nux-vomica, 4 parts of Kusnezoff Monkshood Root, 7 parts of Saururus chinensis, 7 parts of Paris polyphylla, 2 parts of Zanthoxylum nitidum, 7 parts of Angelica sinensis, 7 parts of Carthamus tinctorius, 4 parts of Notopterygium incisum, 4 parts of Willow Bark, 7 parts of Peach Kernel, 4 parts of Chuanxiong Rhizome, 70 parts of Eucommia Bark, 7 parts of Golden Hair Fern Rhizome, 4 parts of Dipsacus Root, and 7 parts of Licorice Root.

[0024] Optionally, the traditional Chinese medicine composition for repairing muscle, tendon and bone injuries is made from the following traditional Chinese medicines in parts by weight: 5 parts of speranskia tuberculata, 1 part of raw nux vomica, 3 parts of kusnezoff monkshood, 5 parts of herba sambuci chinensis, 5 parts of paris polyphylla, 1 part of zanthoxylum nitidum, 5 parts of angelica sinensis, 5 parts of safflower, 3 parts of notopterygium incisum, 3 parts of willow bark, 5 parts of peach kernel, 3 parts of ligusticum wallichii, 5 parts of eucommia ulmoides, 5 parts of cibotium barometz, 3 parts of dipsacus asperoides, and 5 parts of liquorice.

[0025] Optionally, the traditional Chinese medicine composition for repairing muscle, tendon and bone injuries is made from the following traditional Chinese medicines in parts by weight: 10 parts of speranskia tuberculata, 3 parts of raw nux vomica, 5 parts of kusnezoff monkshood, 10 parts of herba sambuci chinensis, 10 parts of paris polyphylla, 3 parts of zanthoxylum nitidum, 10 parts of angelica sinensis, 10 parts of safflower, 5 parts of notopterygium incisum, 5 parts of willow bark, 10 parts of peach kernel, 5 parts of ligusticum wallichii, 10 parts of eucommia ulmoides, 10 parts of cibotium barometz, 5 parts of dipsacus asperoides, and 10 parts of liquorice.

[0026] The present invention also provides a preparation method of the above traditional Chinese medicine composition for repairing muscle, tendon and bone injuries, comprising the following steps:

[0027] Step 1: Weigh the above raw materials, add ethanol, perform ultrasonic extraction, filter, and collect the medicinal residues for later use to obtain filtrate 1;

[0028] Step 2: Centrifuge the filtrate 1 at a low temperature to remove the precipitate to obtain filtrate 2;

[0029] Step 3: Add water to the medicinal residues obtained in Step 1, decoct with slow fire, filter to remove the medicinal residues to obtain filtrate 3;

[0030] Step 3: Mix the filtrate 2 and the filtrate 3, let stand for 6 h, remove the precipitate to obtain the medicinal liquid.

[0031] Optionally, the volume concentration of the ethanol is 50-60%, 7-10 ml of ethanol is added to each gram of the raw materials, and the process parameters of the ultrasonic extraction include: ultrasonic power of 300-500 W, frequency of 40 kHz, temperature of 40-45 °C, and time of 20-30 min.

[0032] Optionally, in Step 1, the filtration is performed using a 0.2-μm ceramic membrane.

[0033] Optionally, the process parameters of the low-temperature centrifugation in Step 2 include: temperature of 4-6 °C, rotation speed of 7000-8000 rpm, and time of 10-15 min.

[0034] Optionally, the temperature of the slow-fire decoction is 80-90 °C, and the time is 0.5-1 h.

[0035] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0036] Based on years of practical experience, the applicant has conducted in-depth research on the etiology and pathogenesis of muscle, tendon, and bone injuries, and believes that the main external causes of this disease are: exogenous pathogenic factors, overstrain and trauma, invasion of the six exogenous pathogens, chronic strain, and acute injury; the main internal causes of muscle, tendon, and bone injuries are: deficiency of vital qi and disharmony of zang-fu organs, deficiency of the liver and kidney, disharmony of qi and blood, dysfunction of the spleen in transportation, accumulation of dampness into phlegm, binding with stasis, obstruction of phlegm and stasis in collaterals, resulting in joint deformity and nodules; the basic pathogenesis of muscle, tendon, and bone injuries is: blockage of meridians and failure of muscles and bones to be nourished, invasion of pathogenic factors such as wind, cold, dampness, and heat into the muscles, tendons, and bones, leading to "unsmooth flow of meridian qi"; abnormal metabolism of qi, blood, and body fluids, congestion of phlegm and stasis in meridians, "failure of qi and blood to reach, resulting in bi pain"; muscles and bones lacking moistening and warming, "not being nourished leads to pain", resulting in chronic dull pain and limited movement; local tissues lacking nourishment, and delayed tissue repair. According to the etiology and pathogenesis of this disease, the treatment principles adopted in the present invention are to dredge the collaterals and relieve pain, promote blood circulation to remove blood stasis, strengthen the muscles and bones, and generate flesh and replenish blood.

[0037] The formula of the present invention follows the principle of compatibility of monarch, minister, assistant, and guide herbs. The whole formula uses speranskia herb, raw nux vomica, kusnezoff monkshood, herba sambuci chinensis, and paris polyphylla as the monarch herbs. Among them: raw nux vomica dredges the collaterals and relieves pain, kusnezoff monkshood dispels cold and relieves pain, speranskia herb penetrates latent pathogenic factors, achieving the effect of dredging the collaterals and relieving pain; herba sambuci chinensis promotes blood circulation and reconnects tendons; paris polyphylla not only has anti-inflammatory and detumescent effects, but also cools the blood and dispels stasis, regulating the warm-dry nature of kusnezoff monkshood and nux vomica to prevent the accumulation of heat-toxicity; using zanthoxylum nitidum, angelica sinensis, safflower, notopterygium root, willow bark, and peach kernel as the minister herbs. Among them: notopterygium root dispels wind and dampness, zanthoxylum nitidum dredges the collaterals and stops convulsions, assisting speranskia herb, raw nux vomica, and kusnezoff monkshood to enhance the effect of dredging the collaterals and relieving pain, relieving pain, and penetrating pathogenic factors; safflower promotes blood circulation and dredges the meridians, peach kernel breaks blood stasis, willow bark dispels stasis and detumescence, assisting herba sambuci chinensis to enhance the effect of promoting blood circulation to remove blood stasis; angelica sinensis nourishes blood and harmonizes the nutrient qi, moistening the muscles and tendons, enhancing the effect of promoting blood circulation to remove blood stasis; using ligusticum wallichii, eucommia ulmoides, cibotium barometz, dipsacus asperoides, and licorice as the assistant and guide herbs. Among them: ligusticum wallichii promotes blood circulation and qi movement, promoting the circulation of medicinal properties; eucommia ulmoides, cibotium barometz, and dipsacus asperoides tonify the liver and kidney, nourishing the muscles and bones; licorice regulates the properties of medicinal herbs. Thus, with "dredging, breaking, tonifying, and regulating" as the main principles, it achieves the effects of dredging the collaterals and relieving pain, promoting blood circulation to remove blood stasis, strengthening the muscles and bones, and generating flesh and replenishing blood.

[0038] The medicinal liquid of the present invention can continuously treat and repair the disease, assisting the gradual repair and recovery of muscles, tendons, and bones, and preventing recurrence. In the acute stage of the disease, the monarch herbs raw nux vomica and kusnezoff monkshood quickly relieve pain, and speranskia herb, together with the minister herbs notopterygium root and zanthoxylum nitidum, dispels wind and cold, breaking through the blockage; paris polyphylla has anti-inflammatory and detumescent effects, cools the blood and dispels stasis, the minister herbs safflower and peach kernel break blood stasis, willow bark dispels stasis and detumescence, and assisted by ligusticum wallichii promoting qi movement, quickly dissipating hematoma; in the repair stage of the disease, the monarch herb herba sambuci chinensis combines with the assistant and guide herbs eucommia ulmoides, cibotium barometz, promoting the regeneration of tendon periosteum; the minister herb angelica sinensis nourishes blood and generates flesh, and the assistant and guide herb dipsacus asperoides continues to connect the injury, reconstructing tissue continuity. In the recovery stage of the disease, the assistant and guide herb licorice regulates various medicinal herbs, reducing the toxicity of kusnezoff monkshood and nux vomica; eucommia ulmoides and cibotium barometz continuously tonify the liver and kidney, preventing recurrence.

[0039] The usage method of the medicinal liquid of the present invention is for external use. It is directly applied to the skin of the affected area. Through the power of penetrating the skin and dredging the collaterals, it reaches the disease location directly, achieving the dynamic balance of "eliminating pathogenic factors without damaging healthy qi, and tonifying deficiency without leaving stasis". The drug molecules in the medicinal liquid quickly penetrate into the body's fascia, muscles, and bones through the skin, enter the blood microcirculation, enabling the drug molecules to reach the lesion directly; dredging the blocked capillaries and relaxing the spasmodic tendons after strain; making the protruding intervertebral disc nucleus pulposus, bone spurs, and hyperplasia and sclerosis of soft tissues caused by bone injury or dislocation gradually soften and dissolve, so that the nerves are no longer compressed, and the symptoms can be fundamentally improved; at the same time, it can also activate the body's self-repair function, repair damaged tissue cells, improve the blood nutrition supply of muscle tissues, and adjust the body's qi, blood, zang-fu organs; the medicinal liquid contacts and is absorbed by the body's fascia, muscles, and bones, thereby achieving the effect of repairing muscles and bones.

[0040] After applying the medicinal liquid to the pain area, it will quickly penetrate into the fascia, muscles, and bones of the affected area. If there is an injured position, it may feel hot, burning, and tingling like being roasted by fire; if there is accumulation of dampness and cold in the affected area, there may be an itchy feeling like being scratched by a cat or bitten by ants. This is a reaction to dispelling wind-cold in the body. The above symptoms can disappear on their own within 10 minutes, and there is no obvious visible change in the skin area, and there will be no allergic or blistering symptoms.

[0041] The function of the medicinal liquid of the present invention is to relieve sub-health caused by damage to the bones and muscles of the neck, shoulders, waist, and legs, including lumbar disc herniation, cervical spondylosis, scapulohumeral periarthritis, lumbar muscle strain, hyperosteogeny, rheumatism, rheumatoid arthritis, gout, sciatica, synovitis and other muscle, tendon, and bone pains.

[0042] Based on the characteristics of the raw materials of the present invention, the preparation method of the present invention combines ultrasonic extraction and slow fire boiling according to the treatment principles of the whole prescription, fully extracts the effective components, significantly helps the efficacy to play, and improves the treatment effect. Detailed implementation mode

[0043] To better understand the present invention, the content of the present invention will be further clearly elaborated below in conjunction with embodiments. However, the protected content of the present invention is not limited to the following embodiments. In the following description, a large number of specific details are given to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details.

[0044] In all the examples shown and discussed here, any specific value should be interpreted as merely exemplary, rather than as a limitation. Therefore, other examples of the exemplary embodiments can have different values.

[0045] Unless otherwise specified, all raw materials are sourced from commercially available products, and unless otherwise specified, they do not contain other components that are not clearly indicated except for inevitable impurities.

[0046] Example 1: A traditional Chinese medicine composition for repairing muscle, tendon and bone injuries is made from the following traditional Chinese medicines in parts by weight: 7 parts of speranskia tuberculata, 2 parts of raw nux vomica, 4 parts of kusnezoff monkshood, 7 parts of herba saururi, 7 parts of paris polyphylla, 2 parts of zanthoxylum nitidum, 7 parts of angelica sinensis, 7 parts of safflower, 4 parts of notopterygium root, 4 parts of willow bark, 7 parts of peach kernel, 4 parts of chuanxiong rhizome, 70 parts of eucommia ulmoides, 7 parts of cibotium barometz, 4 parts of dipsacus asperoides, and 7 parts of liquorice.

[0047] A preparation method of the above-mentioned traditional Chinese medicine composition for repairing muscle, tendon and bone injuries comprises the following steps:

[0048] Step 1: Weigh the above raw materials, add ethanol, extract by ultrasonic wave, filter, collect the medicinal residues for standby, and obtain filtrate 1;

[0049] Step 2: Centrifuge filtrate 1 at low temperature to remove the precipitate, and obtain filtrate 2;

[0050] Step 3: Add water to the medicinal residues obtained in Step 1, decoct with slow fire, filter to remove the medicinal residues, and obtain filtrate 3;

[0051] Step 4: Mix filtrate 2 and filtrate 3, let stand for 6 h, remove the precipitate, and obtain the medicinal liquid.

[0052] Among them, the volume concentration of ethanol is 50%, 8 ml of ethanol is added to each gram of raw material, and the process parameters of ultrasonic extraction include: ultrasonic power is 400 W, frequency is 40 kHz, temperature is 40 °C, and time is 30 min.

[0053] In Step 1, filtration is carried out using a 0.2-μm ceramic membrane.

[0054] The process parameters of centrifugation at low temperature in Step 2 include: temperature 4 °C, rotation speed 8000 rpm, and time 10 min.

[0055] 5 ml of water is added to each gram of raw material, the temperature of decocting with slow fire is 90 °C, and the time is 0.5 h.

[0056] Example 2: A traditional Chinese medicine composition for repairing muscle, tendon and bone injuries is made from the following traditional Chinese medicines in parts by weight: 5 parts of speranskia tuberculata, 1 part of raw nux vomica, 3 parts of kusnezoff monkshood, 5 parts of herba saururi, 5 parts of paris polyphylla, 1 part of zanthoxylum nitidum, 5 parts of angelica sinensis, 5 parts of safflower, 3 parts of notopterygium root, 3 parts of willow bark, 5 parts of peach kernel, 3 parts of chuanxiong rhizome, 5 parts of eucommia ulmoides, 5 parts of cibotium barometz, 3 parts of dipsacus asperoides, and 5 parts of liquorice.

[0057] A preparation method of the above-mentioned traditional Chinese medicine composition for repairing muscle, tendon and bone injuries comprises the following steps:

[0058] Step 1: Weigh the above raw materials, add ethanol, extract by ultrasonic wave, filter, collect the medicinal residues for standby, and obtain filtrate 1;

[0059] Step 2: Centrifuge the first filtrate at low temperature to remove the precipitate, obtaining the second filtrate;

[0060] Step 3: Add water to the medicinal residues from Step 1, decoct with gentle heat, filter to remove the medicinal residues, obtaining the third filtrate;

[0061] Step 3: Mix the second filtrate and the third filtrate, let it stand for 6 h, remove the precipitate, obtaining the medicinal liquid.

[0062] Among them, the volume concentration of ethanol is 60%, 7 ml of ethanol is added per gram of the crude drug, and the process parameters of ultrasonic extraction include: ultrasonic power of 500 W, frequency of 40 kHz, temperature of 45 °C, and time of 20 min.

[0063] In Step 1, the filtration is carried out using a 0.2 - um ceramic membrane.

[0064] The process parameters of centrifugation at low temperature in Step 2 include: temperature of 6 °C, rotation speed of 8000 rpm, and time of 12 min.

[0065] 6 ml of water is added per gram of the crude drug, the temperature of decocting with gentle heat is 80 °C, and the time is 1 h.

[0066] Example 3: A traditional Chinese medicine composition for repairing muscle, tendon and bone injuries is made from the following traditional Chinese medicines in parts by weight: 10 parts of Speranskia tuberculata, 3 parts of Strychnos nux - vomica, 5 parts of Kusnezoff Monkshood Root, 10 parts of Saururus chinensis, 10 parts of Paris polyphylla, 3 parts of Zanthoxylum nitidum, 10 parts of Angelica sinensis, 10 parts of Carthamus tinctorius, 5 parts of Notopterygium incisum, 5 parts of Willow Bark, 10 parts of Prunus persica, 5 parts of Ligusticum wallichii, 10 parts of Eucommia ulmoides, 10 parts of Cibotium barometz, 5 parts of Dipsacus asperoides, 10 parts of Glycyrrhiza uralensis.

[0067] A preparation method of the above - mentioned traditional Chinese medicine composition for repairing muscle, tendon and bone injuries comprises the following steps:

[0068] Step 1: Weigh the above - mentioned crude drugs, add ethanol, carry out ultrasonic extraction, filter, collect the medicinal residues for standby, obtaining the first filtrate;

[0069] Step 2: Centrifuge the first filtrate at low temperature to remove the precipitate, obtaining the second filtrate;

[0070] Step 3: Add water to the medicinal residues from Step 1, decoct with gentle heat, filter to remove the medicinal residues, obtaining the third filtrate;

[0071] Step 3: Mix the second filtrate and the third filtrate, let it stand for 6 h, remove the precipitate, obtaining the medicinal liquid.

[0072] Among them, the volume concentration of ethanol is 55%, 10 ml of ethanol is added per gram of the crude drug, and the process parameters of ultrasonic extraction include: ultrasonic power of 300 W, frequency of 40 kHz, temperature of 45 °C, and time of 30 min.

[0073] In Step 1, filtration is carried out using a 0.2-μm ceramic membrane.

[0074] In Step 2, the process parameters for centrifugation at low temperature include: temperature 4°C, rotation speed 7000 rpm, and time 15 min.

[0075] Add 5 ml of water to each gram of the raw drug, and the temperature for slow boiling over a gentle flame is 85°C, and the time is 0.8 h.

[0076] Comparative Example 1: A traditional Chinese medicine composition for repairing muscle, tendon and bone injuries. The difference from Example 1 is that: Lippia nodiflora is used to replace Paris polyphylla, and the other raw materials and parts by weight remain unchanged. Among them, Lippia nodiflora is a plant of the genus Lippia in the family Verbenaceae, Lippia nodiflora (L.) Greene [Lippia nodiflora (L.) Rich.], and its whole herb is used as medicine, clearing heat and detoxifying, dispersing stasis and reducing swelling, and has similar effects to Paris polyphylla.

[0077] Comparative Example 2: A traditional Chinese medicine composition for repairing muscle, tendon and bone injuries. The difference from Example 1 is that its preparation method includes the following steps:

[0078] Step 1: Weigh the raw drug, add water, and slowly boil over a gentle flame, filter to remove the drug residues to obtain a filtrate;

[0079] Step 2: Let the filtrate stand for 6 h, remove the precipitate to obtain a medicinal liquid.

[0080] Among them, add 8 ml of water to each gram of the raw drug;

[0081] The temperature for slow boiling over a gentle flame is 90°C, and the time is 2 h.

[0082] Effect Example 1: Skin acute toxicity and irritation tests

[0083] 1.1 Skin acute toxicity test: Select 12 healthy white rabbits, weighing 1.8 - 2.0 kg, with half males and half females. Randomly divide them into a complete skin group and a damaged skin group according to body weight, with two parallels in each group and 3 rabbits in each group.

[0084] Complete skin group: 24 h before the experiment, use scissors and a hair clipper to shave off the hair on the left and right sides of the back of the test rabbits by about 4 cm × 5 cm area, ensuring that the skin is intact and undamaged.

[0085] Damaged skin group: 24 h before the experiment, use scissors and a hair clipper to shave off the hair on the left and right sides of the back of the test rabbits by about 4 cm × 5 cm area. After disinfecting the test area, gently scratch the skin with a five-edge knife until slight bleeding occurs.

[0086] Apply 5 ml of the medicinal liquid prepared in Example 1 to the skin of rabbits and smear for 24 hours. After the experiment, wash off the remaining test substance with warm water. Observe the changes in the skin, hair, eyes and mucous membranes of rabbits, as well as the systemic toxic manifestations such as respiration, central nervous system, limb movements, etc. and the death condition at 1 h, 24 h, 48 h and 72 h to 7 d after removing the test substance.

[0087] Experimental results: Within the 7-day observation period, the rabbits moved freely, and there were no abnormalities in the mental state, appetite, and defecation. None of the rabbits died. The medicinal liquid prepared in Example 1 did not cause acute toxicity reactions when applied externally to the intact and damaged skin of rabbits.

[0088] 1.2 Acute skin irritation test: Select 12 healthy white rabbits with a body weight of 1.8 - 2.0 kg, half male and half female. Randomly divide them into an intact skin group and a damaged skin group according to body weight, with two parallels in each group and 3 rabbits in each group.

[0089] Intact skin group: 24 h before the experiment, use scissors and a hair clipper to shave off the hair on both sides of the spine of the test rabbits on the back with an area of about 5 cm × 10 cm, ensuring that the skin is intact without damage.

[0090] Damaged skin group: 24 h before the experiment, use scissors and a hair clipper to shave off the hair on both sides of the spine of the test rabbits on the back with an area of about 5 cm × 10 cm. After disinfecting the test area, gently scratch the skin with a five-edge knife until slight bleeding occurs.

[0091] Apply 5 ml of the medicinal liquid prepared in Example 1 to the skin of rabbits and smear for 5 hours. After the experiment, remove the remaining test substance with warm water. Observe the skin reaction at the smeared area at 1 h, 24 h, 48 h and 72 h after removing the test substance.

[0092] Experimental results: No irritation reactions such as erythema and edema were observed in the skin of the test area in the intact skin group, and it was not irritating to the intact skin of rabbits. In the damaged skin group, erythema was barely visible at the beginning of smearing the test substance and disappeared within 1 h of smearing, and there were no other irritation reactions such as edema.

[0093] Effect Example 2: Pharmacodynamic Test of the Repair Liquid Medicine of the Present Invention

[0094] 1 Data and methods

[0095] 1.1 General information A total of 120 patients with muscle, tendon, and bone pain admitted to Jiaheng Health Zhengde Traditional Chinese Medicine Clinic from January 1, 2024, to December 31, 2024, were selected. They were divided into a control group, an observation group, a comparison group 1, and a comparison group 2 by the random number table method, with 30 cases in each group. In the control group, there were 15 males and 15 females, aged 40 - 60 years. In the observation group, there were 14 males and 16 females, aged 38 - 60 years. In the comparison group 1, there were 16 males and 14 females, aged 37 - 58 years. In the comparison group 2, there were 15 males and 15 females, aged 41 - 60 years. There was no significant difference in the general information among the four groups (P > 0.05), and they were comparable.

[0096] 1.2 Diagnostic criteria

[0097] 1.2.1 Western medicine According to the diagnostic criteria for myofascial pain syndrome in the "Clinical Practice Guidelines: Physical Medicine and Rehabilitation Sub - volume" (Reference: Li Jing, Tan Weiyi, Li Jian'an, et al. Clinical Practice Guidelines (Physical Medicine and Rehabilitation Sub - volume) [M]. Beijing: People's Medical Publishing House, 2005: 71 - 72.).

[0098] 1.2.2 Traditional Chinese medicine Refer to the diagnostic criteria for bi - syndrome in the "Diagnostic and Therapeutic Criteria for Traditional Chinese Medicine Syndromes" (Reference: State Administration of Traditional Chinese Medicine. Diagnostic and Therapeutic Criteria for Traditional Chinese Medicine Syndromes [S]. Nanjing: Nanjing University Press, 1994: 201.).

[0099] 1.3 Inclusion and exclusion criteria

[0100] 1.3.1 Inclusion criteria (1) Meet the diagnostic criteria under item "1.2"; (2) The course of disease exceeds 3 months; (3) The patient and his / her family accept the treatment and sign the informed consent form.

[0101] 1.3.2 Exclusion criteria (1) Complicated with diseases of the heart, liver, kidney and other systems; (2) Having a history of depression or mania; (3) Having an allergic history to the test drugs.

[0102] 1.4 Treatment methods The control group used diclofenac diethylamine emulsion (Voltaren) externally. The amount was taken according to the size of the painful area, once in the morning, once at noon, and once in the evening. The observation group, the comparison group 1, and the comparison group 2 used the medicinal liquids prepared in Example 1, Comparative Example 1, and Comparative Example 2 externally, with a dosage of 5 mL / 50 cm 2 , once in the morning, once at noon, and once in the evening. Each of the 4 groups took 2 weeks as a treatment course and continuously treated for 3 courses.

[0103] 1.5 Index detection The visual analogue scale (VAS) was used to evaluate the degree of musculoskeletal pain before and after treatment in four groups. The VAS score was evaluated using a 10-cm vernier caliper to assess the pain degree, where: 0 points: painless; 1-3 points: mild pain; 4-6 points: moderate pain; 7-10 points: severe pain. The quality of life evaluation scale (SF-36) was used to evaluate the quality of life in different dimensions after treatment in four groups, and the score was positively correlated with the quality of life.

[0104] 1.6 Efficacy evaluation It was carried out with reference to the Diagnostic and Therapeutic Criteria for TCM Syndromes and Diseases. (1) Cure: The subjective symptoms and clinical signs disappeared, and the patient could resume normal work and life, with VAS score ≤ 3 points; (2) Improvement: Most of the symptoms and signs disappeared, and the patient could basically resume the original work and life, with 3 points < VAS score ≤ 6 points; (3) Ineffective: The symptoms and signs did not improve, with VAS score > 6 points. The total effective rate = [(number of cured cases + number of improved cases) / total number of cases] × 100%.

[0105] 1.7 Statistical analysis It was processed by SPSS 25.0 software. Measurement data were expressed as and for those with normal distribution and homogeneous variance, independent sample t-test was used for inter-group comparison, and paired sample t-test was used for intra-group comparison. For those with skewed distribution and inhomogeneous variance, rank sum test was used for inter-group comparison; count data were expressed as percentages, and chi-square test was used for inter-group comparison; P < 0.05 indicated that the difference was statistically significant.

[0106] 2 Results

[0107] 2.1 VAS score

[0108] Table 1 Comparison of VAS scores of 4 groups (points, n = 30)

[0109] Group Before Treatment After Treatment Observation Group 6.72±1.31 <![CDATA[2.41±0.86 ☆△ > Control Group 6.64±1.28 <![CDATA[4.05±1.02 ☆ > Comparison Group 1 6.67±1.34 <![CDATA[3.57±0.95 ☆ > Comparison Group 2 6.75±1.27 <![CDATA[3.34±1.13 ☆ >

[0110] Note: Compared with before treatment in the same group, ☆ P < 0.05, compared with after treatment in the control group, △ P < 0.05.

[0111] The results showed that after treatment, the VAS scores of the 4 groups all decreased (P < 0.05), and the effect of the observation group was more significant (P < 0.05).

[0112] 2.2 SF-36 score

[0113] Table 2 Comparison of SF-36 scores of 4 groups (points, n = 30)

[0114] Group Before Treatment After Treatment Observation Group 60.12±7.03 <![CDATA[83.25±5.12 ☆△ > Control Group 59.45±7.54 <![CDATA[72.36±6.04 ☆ > Comparison Group 1 61.27±6.83 <![CDATA[76.41±6.72 ☆ > Comparison Group 2 60.84±7.36 <![CDATA[79.16±5.84 ☆ >

[0115] Note: Compared with before treatment in the same group, ☆ P < 0.05. Compared with after treatment in the control group, △ P < 0.05.

[0116] The results showed that after treatment, the SF-36 scores of all 4 groups increased (P < 0.05), and the effect was more significant in the observation group (P < 0.05).

[0117] 2.3 Efficacy of traditional Chinese medicine syndromes

[0118] Table 3 Comparison of the efficacy of traditional Chinese medicine syndromes in 4 groups [cases (%), n = 30]

[0119] Group Cured Improved Invalid Total Effective Observation Group <![CDATA[12(40.0) △ > 13(43.3) 5(16.7) <![CDATA[25(83.3) △ > Control Group 9(30.0) 12(40.0) 9(30.0) 21(70.0) Comparison Group 1 8(26.7) 14(46.7) 8(26.7) 22(73.3) Comparison Group 2 10(33.3) 14(46.7) 6(20.0) <![CDATA[24(80.0) △ >

[0120] Note: Compared with the control group, △ P < 0.05.

[0121] The results showed that the total effective rates of the observation group and the comparison group 2 were both higher than that of the control group (P < 0.05), and the cure rate of the observation group was higher than that of the control group (P < 0.05).

[0122] It can be seen that using the liquid medicine of the present invention can achieve the effects of dredging collaterals and relieving pain, promoting blood circulation and removing blood stasis, strengthening tendons and bones, and generating muscle and enriching blood, and can improve the quality of life of patients in many aspects such as physical function, physical role, muscle pain, health status, social function, emotional role and mental health, with remarkable curative effects.

[0123] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Any other modifications or equivalent replacements made by those of ordinary skill in the art to the technical solutions of the present invention shall be covered by the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solutions of the present invention.

Claims

1. A traditional Chinese medicine composition for repairing muscle, tendon and bone injuries, characterized in that: It is made from the following Chinese herbal medicines by weight: 5 - 10 parts of Speranskia tuberculata, 1 - 3 parts of raw Strychnos nux-vomica, 3 - 5 parts of Kusnezoff Monkshood Root, 5 - 10 parts of Saururus chinensis, 5 - 10 parts of Paris polyphylla, 1 - 3 parts of Zanthoxylum nitidum, 5 - 10 parts of Angelica sinensis, 5 - 10 parts of Carthamus tinctorius, 3 - 5 parts of Notopterygium incisum, 3 - 5 parts of Willow bark, 5 - 10 parts of Prunus persica, 3 - 5 parts of Ligusticum wallichii, 5 - 10 parts of Eucommia ulmoides, 5 - 10 parts of Cibotium barometz, 3 - 5 parts of Dipsacus asperoides, 5 - 10 parts of Glycyrrhiza uralensis Fisch.

2. The traditional Chinese medicine composition for repairing muscle, tendon and bone injuries as described in claim 1, wherein: It is made from the following Chinese herbal medicines by weight: 7 parts of Speranskia tuberculata, 2 parts of raw Strychnos nux-vomica, 4 parts of Kusnezoff Monkshood Root, 7 parts of Saururus chinensis, 7 parts of Paris polyphylla, 2 parts of Zanthoxylum nitidum, 7 parts of Angelica sinensis, 7 parts of Carthamus tinctorius, 4 parts of Notopterygium incisum, 4 parts of Willow bark, 7 parts of Prunus persica, 4 parts of Ligusticum wallichii, 70 parts of Eucommia ulmoides, 7 parts of Cibotium barometz, 4 parts of Dipsacus asperoides, 7 parts of Glycyrrhiza uralensis Fisch.

3. The traditional Chinese medicine composition for repairing muscle, tendon and bone injuries as described in claim 1, wherein: The traditional Chinese medicine composition for repairing muscle, tendon and bone injuries is made from the following Chinese herbal medicines by weight: 5 parts of Speranskia tuberculata, 1 part of raw Strychnos nux-vomica, 3 parts of Kusnezoff Monkshood Root, 5 parts of Saururus chinensis, 5 parts of Paris polyphylla, 1 part of Zanthoxylum nitidum, 5 parts of Angelica sinensis, 5 parts of Carthamus tinctorius, 3 parts of Notopterygium incisum, 3 parts of Willow bark, 5 parts of Prunus persica, 3 parts of Ligusticum wallichii, 5 parts of Eucommia ulmoides, 5 parts of Cibotium barometz, 3 parts of Dipsacus asperoides, 5 parts of Glycyrrhiza uralensis Fisch.

4. The traditional Chinese medicine composition for repairing muscle, tendon and bone injuries according to claim 1, wherein: The traditional Chinese medicine composition for repairing muscle, tendon and bone injuries is made from the following Chinese herbal medicines by weight: 10 parts of Speranskia tuberculata, 3 parts of raw Strychnos nux-vomica, 5 parts of Kusnezoff Monkshood Root, 10 parts of Saururus chinensis, 10 parts of Paris polyphylla, 3 parts of Zanthoxylum nitidum, 10 parts of Angelica sinensis, 10 parts of Carthamus tinctorius, 5 parts of Notopterygium incisum, 5 parts of Willow bark, 10 parts of Prunus persica, 5 parts of Ligusticum wallichii, 10 parts of Eucommia ulmoides, 10 parts of Cibotium barometz, 5 parts of Dipsacus asperoides, 10 parts of Glycyrrhiza uralensis Fisch.

5. The preparation method of a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries as described in any one of claims 1-4, characterized in that: It includes the following steps: Step 1: Weigh the above raw materials, add ethanol, extract by ultrasonic wave, filter, collect the medicinal residues for standby, and obtain filtrate 1; Step 2: Centrifuge the filtrate 1 at low temperature to remove the precipitate, and obtain filtrate 2; Step 3: Add water to the medicinal residues obtained in Step 1, decoct with slow fire, filter to remove the medicinal residues, and obtain filtrate 3; Step 3: Mix the filtrate 2 and the filtrate 3, let it stand, remove the precipitate, and obtain the medicinal liquid.

6. The preparation method of a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries as described in claim 5, characterized in that: The volume concentration of the ethanol is 50 - 60%, and 7 - 10 ml of ethanol is added to each gram of the raw materials.

7. The preparation method of a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries as described in claim 5, characterized in that: The process parameters of the ultrasonic extraction include: ultrasonic power of 300 - 500 W, frequency of 40 kHz, temperature of 40 - 45 °C, and time of 20 - 30 min.

8. The preparation method of a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries as described in claim 5, characterized in that: In Step 1, the filtration is carried out using a 0.2 - um ceramic membrane.

9. The preparation method of a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries as described in claim 5, characterized in that: The process parameters of the low-temperature centrifugation in Step 2 include: temperature of 4 - 6 °C, rotation speed of 7000 - 8000 rpm, and time of 10 - 15 min.

10. The preparation method of a traditional Chinese medicine composition for repairing muscle, tendon and bone injuries as described in claim 5, characterized in that: The temperature of the slow-fire decoction is 80 - 90 °C, and the time is 0.5 - 1 h.