Textile antibacterial method based on low-frequency pulsed magnetic field

Abstract

The invention provides a textile antibacterial treatment method based on a low-frequency pulsed magnetic field, which can enable an operator to online detect the antibacterial condition of textile antibacterial treatment equipment in real time and control and adjust an antibacterial pulsed magnetic field and ensure the antibacterial effect of the antibacterial treatment equipment according to the antibacterial condition.

Description

Technical Field

[0001] The present invention relates to the technical field of antibacterial treatment of fiber fabrics, and particularly to an antibacterial method for textiles based on low-frequency pulsed magnetic fields, enabling operators to detect the antibacterial situation of textiles in real time online and control and adjust the antibacterial pulsed magnetic field according to the antibacterial situation to ensure the antibacterial effect of the antibacterial treatment equipment and accurately control the antibacterial time. Background Art

[0002] Traditional antibacterial (or sterilization) technologies each have many defects or application limitations. For example, ultraviolet sterilization has low ultraviolet penetration ability and can only sterilize the surface of objects, making it difficult to effectively sterilize stacked objects, especially stacked textiles; chemical sterilization requires the use of a large amount of chemical reagents, and after sterilization, the removal of chemical reagents is a major problem, and the chemical reagents themselves may also cause pollution and even hygiene problems; high-temperature steam sterilization technology not only has a high temperature and high energy consumption, but also requires a large working space, and high-temperature steam may also damage textiles.

[0003] Pulsed magnetic field antibacterial technology is a new type of non-thermal antibacterial technology that uses pulsed magnetic fields with short pulse widths (0 - 100 μs) to process liquids, semi-solids, or solid objects including textiles to achieve non-destructive antibacterial or sterilization. Compared with traditional sterilization technologies, pulsed magnetic field antibacterial equipment occupies less space, has low energy consumption, is simple in structure, easy to control, has no impact on textiles before and after antibacterial treatment, and does not cause any environmental pollution, and is welcomed by users, especially users in industries such as clothing manufacturers and hotels.

[0004] However, there are also major problems with existing pulsed magnetic field antibacterial technologies: factors such as environmental temperature and air humidity will affect the antibacterial effect of pulsed magnetic fields, and most existing pulsed magnetic field antibacterial treatment equipment cannot quickly and timely determine the antibacterial effect, resulting in either insufficient antibacterial time (or degree) and unable to obtain a good antibacterial effect, or too long antibacterial time, leading to excessive energy consumption and waste, and even significantly increasing the time cost of users. There may even be a situation where due to the inability to timely determine the antibacterial effect of pulsed magnetic fields, long-term ineffective antibacterial treatment occurs, wasting energy and time in vain. Secondly, most existing pulsed magnetic field antibacterial technologies use high-energy and high-intensity pulsed magnetic fields with high electric field intensities (10 - 50 kV / cm) and high pulse frequencies (above 2000 Hz) to process objects. High-energy and high-intensity magnetic fields have a greater impact on the human body and may pose a danger to operators. Summary of the Invention

[0005] The main advantage of the present invention is to provide a method for antibacterial treatment of textiles based on low-frequency pulsed magnetic fields, which enables operators to detect the antibacterial situation of textile antibacterial treatment equipment online in real time, control and adjust the antibacterial pulsed magnetic field according to the antibacterial situation, and ensure the antibacterial effect of the antibacterial treatment equipment. Accordingly, the method for antibacterial treatment of textiles based on low-frequency pulsed magnetic fields of the present invention enables operators to more precisely control the antibacterial time, avoiding over-antibacterial and energy waste.

[0006] Other objects and features of the present invention are fully embodied in the following detailed description.

[0007] According to one aspect of the present invention, the present invention provides a method for antibacterial treatment of textiles based on low-frequency pulsed magnetic fields, which includes:

[0008] Place the textile to be treated in the magnetic flux space, put the sample plate into the chamber of the sample calibration chamber of the textile antibacterial equipment, and close the chamber door;

[0009] Through the microscopic camera and fluorescence camera of the imaging device of the sample calibration system, under the guidance of the bacterial cell distribution map of the detection surface of the calibrated textile sample, perform fluorescence imaging and microscopic imaging on the bacteria on the detection surface of the calibrated textile sample laid flat on the sample plate;

[0010] Randomly select at least 10 clearly imaged single-distributed bacteria as the calibrated bacterial cells, and generate a first real-time detection image of the calibrated bacterial cells according to the position distribution of the calibrated bacterial cells;

[0011] Start the antibacterial equipment, supply power to the magnetic coils of the antibacterial equipment, so that the magnetic coils can generate a preset pulsed magnetic field and perform antibacterial treatment on the textiles;

[0012] Every 5-10 minutes of antibacterial treatment, through the microscopic camera and the fluorescence camera of the imaging device of the sample calibration system, detect again the number and morphology of the bacterial cells at the positions of the at least 10 clearly imaged single-distributed bacteria randomly selected, so as to obtain the Nth real-time detection image; and

[0013] Compare the number and morphology of the bacterial cells at the positions of the at least 10 clearly imaged single-distributed bacteria randomly selected in the first real-time detection image and the Nth real-time detection image. If the number of the bacterial cells at the positions of the at least 10 clearly imaged single-distributed bacteria randomly selected increases, change at least one variable of the pulse width, pulse frequency, and electric field strength of the preset pulsed magnetic field, and re-perform antibacterial treatment on the textiles in the magnetic flux space.

[0014] Through the understanding of the subsequent description and drawings, further objects and advantages of the present invention will be fully embodied.

[0015] These and other objects, features, and advantages of the present invention will be fully embodied by the following detailed description, the accompanying drawings, and the claims. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 FIG. 8 is a three-dimensional schematic view of a textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to an embodiment of the present invention.

[0017] Figure 2 FIG. 9 is another three-dimensional schematic view of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention.

[0018] Figure 3 FIG. 10 is a partial cross-sectional schematic view of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention.

[0019] Figure 4 FIG. 11 is another partial cross-sectional schematic view of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention, showing the sample calibration system of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field of the present invention.

[0020] Figure 5 FIG. 12 is another partial cross-sectional schematic view of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention, showing the magnetic coils of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field of the present invention.

[0021] Figure 6 FIG. 13 is a three-dimensional schematic view of the sample calibration system of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention.

[0022] Figure 7 FIG. 14 is a cross-sectional schematic view of the sample calibration system of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention.

[0023] Figure 8 FIG. 15 is another cross-sectional schematic view of the sample calibration system of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention.

[0024] Figure 9 FIG. 16 is a three-dimensional schematic view of the camera device of the sample calibration system of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention.

[0025] Figure 10 FIG. 17 is a structural schematic view of the sample calibration system of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the embodiment of the present invention.

[0026] Figure 11It is a flowchart of the textile antibacterial method based on low-frequency pulsed magnetic field according to the embodiments of the present invention above. Detailed implementation manners

[0027] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments in the following description are only examples, and other obvious variations can be conceived by those skilled in the art. The basic principles defined in the following description of the present invention can be applied to other embodiments, variations, improvements, equivalent solutions, and other technical solutions without departing from the spirit and scope of the present invention.

[0028] Those skilled in the art should understand that in the disclosure of the present invention, the orientation or positional relationship indicated by terms such as "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the above terms should not be construed as limiting the present invention.

[0029] It can be understood that the term "one" should be understood as "at least one" or "one or more". That is, in one embodiment, the number of an element can be one, while in other embodiments, the number of the element can be multiple. The term "one" should not be construed as a limitation on the quantity.

[0030] Referring to the accompanying drawings of the specification Figures 1 to 10, the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to an embodiment of the present invention is illustrated, wherein the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention includes an outer housing 10, an inner housing 20, a frame 30 and a magnetic field generating unit 40, wherein the magnetic field generating unit 40 includes a magnetic force coil 41 and a power supply control device 42, wherein the frame 30 is disposed between the outer housing 10 and the inner housing 20, the magnetic force coil 41 is disposed between the frame 30 and the outer housing 10, and the magnetic force coil 41 is disposed around the frame 30, wherein the inner housing 20 forms a magnetic flux space 201, the magnetic force coil 41 is disposed around the magnetic flux space 201, wherein the power supply control device 42 is electrically connected to an external power supply and the magnetic force coil 41 respectively, so that it can supply a preset alternating current to the magnetic force coil 41 by using the external power supply and make it generate a low-frequency pulsed magnetic field. It can be understood that the low frequency herein refers to that the highest frequency of the alternating current passing through the magnetic force coil 41 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention does not exceed 1000 Hz. Preferably, the magnetic force coil 41 forms two Helmholtz coils connected in series, so as to generate a uniform pulsed magnetic field in the magnetic flux space 201. Correspondingly, the textile can be placed in the magnetic flux space 201 and subjected to antibacterial treatment by the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention.

[0031] Those skilled in the art can understand that the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention can produce antibacterial and sterilization effects through two aspects of action mechanisms: First, the low-frequency pulsed magnetic field generated by the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention can cause the groups on the textile fiber molecules to undergo energy transitions. After the groups on the textile fiber molecules undergo energy transitions, the generated ions will react with the surface layer of bacteria attached to the molecular fibers and prevent the bacteria from carrying out normal basic metabolism, thereby playing a role in inhibiting the proliferation of bacteria. Second, the low-frequency pulsed magnetic field can also directly affect the normal proliferation of bacteria in various ways. For example, the low-frequency pulsed magnetic field may electrolyze polysaccharides or proteins in the bacterial cells, release a variety of free radicals that damage the cell structure of the bacteria, resulting in electroporation and inactivation of the bacterial cells; the low-frequency pulsed magnetic field may also change the membrane potential of the bacteria, cause damage to the cell membrane and lead to the inactivation of the bacteria; cause the bacterial cells to release excessive charged ions, such as calcium ions, which affect the normal operation of transmembrane proteins in the bacterial cell membrane. The above actions of the low-frequency pulsed magnetic field can all inhibit the proliferation of bacteria and even kill the bacteria.

[0032] As shown in the accompanying drawings Figures 3 to 10As shown, the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to an embodiment of the present invention further includes a sample calibration system 50 disposed in the magnetic flux space 201, wherein the sample calibration system 50 includes a camera device 51 and a sample calibration component 52. The sample calibration component 52 includes at least one calibrated textile sample 521, and the calibrated textile sample 521 is laid flat horizontally. The camera device 51 is disposed directly above the calibrated textile sample 521 so that the camera device 51 can image the detection surface 5211 of the calibrated textile sample 521 and identify the number and morphology of bacteria on the detection surface 5211 of the calibrated textile sample 521. Preferably, the camera device 51 is a variable-focus camera device. Those skilled in the art can understand that when the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention performs antibacterial treatment on textiles disposed in the magnetic flux space 201, especially textiles stacked together, it is very difficult to detect its antibacterial effect. The present invention can determine the change in the number and morphology of bacteria on the detection surface 5211 of the calibrated textile sample 521 of the sample calibration component 52 prepared in advance through on-line real-time detection at different times, so as to indirectly detect the antibacterial effect of the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention on the textiles disposed in the magnetic flux space 201. For example, if during the detection, the number of bacteria on the detection surface 5211 of the calibrated textile sample 521 of the sample calibration component 52 increases significantly, it can be determined that the proliferation of bacteria on the detection surface 5211 of the calibrated textile sample 521 of the sample calibration component 52 is not effectively inhibited; if during the detection, the number of bacteria on the detection surface 5211 of the calibrated textile sample 521 of the sample calibration component 52 does not change or basically does not change, and even a large number of bacteria die and lyse, it can be determined that the proliferation of bacteria on the detection surface 5211 of the calibrated textile sample 521 of the sample calibration component 52 is effectively inhibited and the antibacterial effect is good.

[0033] As shown in the accompanying drawings Figures 3 to 10As shown, specifically, the imaging device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to an embodiment of the present invention includes a fluorescence camera 511, a microscopic camera 512, and a data processing and control device 513. The microscopic camera 512 and the fluorescence camera 511 are respectively electrically connected to the data processing and control device 513. The data processing and control device 513 can obtain a fluorescence image of the bacterial cells on the detection surface 5211 of the calibration textile sample 521 through the fluorescence camera 511 and obtain a microscopic image of the bacterial cells on the detection surface 5211 of the calibration textile sample 521 by controlling the microscopic camera 512, and identify the quantity and morphology of the bacteria on the detection surface 5211 of the calibration textile sample 521 according to the microscopic image of the bacterial cells obtained by the microscopic camera 512. Further, the orientations of the microscopic camera 512 and the fluorescence camera 511 are both set to be adjustable so that there is an overlap between the imaging areas of the microscopic camera 512 and the fluorescence camera 511 and a common imaging area is generated. Further, the data processing and control device 513 is set to be able to control and adjust the orientations of the microscopic camera 512 and the fluorescence camera 511 through a driving component so that there is an overlap between the imaging area of the microscopic camera 512 and the imaging area of the fluorescence camera 511 and a common imaging area is generated. Further, the data processing and control device 513 is set to be able to control and adjust the distances between the microscopic camera 512 and the fluorescence camera 511 and the detection surface 5211 of the calibration textile sample 521 through a driving component so that the imaging areas of the microscopic camera 512 and the fluorescence camera 511 can be focused and clearly image the detection surface 5211 of the calibration textile sample 521. Preferably, the magnification of the fluorescence camera 511 is 100-400 times, and the magnification of the microscopic camera 512 is 400-1000 times. Preferably, the microscopic camera 512 is an achromatic microscopic camera. It can be understood that the data processing and control device 513 can be a computer or a mobile electronic device, or may be other devices or control components that can implement control functions. The data processing and control device 513 and the imaging device 51 can be connected by wire or wirelessly so that it can online control the orientations of the microscopic camera 512 and the fluorescence camera 511 to image the detection surface 5211 of the calibration textile sample 521, thereby online and real-time detecting the quantity and morphology of the bacteria on the detection surface 5211 of the calibration textile sample 521.It can be understood that the data processing and control device 513 at least includes a data processing module, a power supply control device, and a driving component. The data processing module of the data processing and control device 513 can form an image based on the optoelectronic signals obtained by the photosensitive elements of the microscopic camera 512 and the fluorescence camera 511. The power supply control device and the driving component can control the orientations of the microscopic camera 512 and the fluorescence camera 511 so that the imaging areas of the microscopic camera 512 and the fluorescence camera 511 overlap and generate the common imaging area. Correspondingly, in the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic fields of the present invention, the fluorescence camera 511 of the imaging device 51 can perform fluorescence imaging on the fluorescently stained bacteria on the surface of the textile sample located in the common imaging area, and the microscopic camera 512 can perform microscopic imaging on the fluorescently stained bacteria on the surface of the textile sample located in the common imaging area. The fluorescence signal of bacteria stained with a fluorescent dye is stronger than that of ordinary visible light imaging, but the fluorescence signal of stained bacteria often makes it difficult to clearly display the microscopic morphology of bacteria, and the positions of multiple fluorescence signals will form fluorophores, preventing the fluorescence camera 511 from obtaining a clear image of the bacteria, such as the flagella on the surface of bacteria or the clear morphology of bacteria, while the imaging signal of the visible light imaging of bacteria by the microscopic camera 512 is poor, making it difficult to quickly locate the position of the bacteria and form an image. Therefore, when the imaging device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic fields of the present invention images the bacteria on the detection surface 5211 of the calibrated textile sample 521, the fluorescence camera 511 can first perform fluorescence imaging on the stained bacteria at a lower magnification to quickly distinguish and locate the single-distributed bacteria, and then the microscopic camera 512 can perform microscopic imaging on the distinguished and located single bacteria.

[0034] Further, in order to enable the imaging device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention to detect the number and morphology of bacteria on the detection surface 5211 of the calibration textile sample 521 in real time online at a faster speed, the distribution of bacteria on the entire detection surface 5211 of the calibration textile sample 521 can be detected first to obtain a bacterial cell distribution map of the entire detection surface 5211 of the calibration textile sample 521. For the convenience of the fluorescence camera 511 of the imaging device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention to perform bacterial fluorescence detection and positioning on the entire detection surface 5211 of the calibration textile sample 521 in subsequent steps, the bacterial cell distribution map of the entire detection surface 5211 of the calibration textile sample 521 should be made by fluorescence imaging, and the imaging magnification is the same as the magnification of the fluorescence camera 511 of the imaging device 51 of the subsequent sample calibration system 50. Correspondingly, when the fluorescence camera 511 of the imaging device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention performs bacterial fluorescence detection and positioning on the entire detection surface 5211 of the calibration textile sample 521, the bacterial fluorescence detection and positioning on the entire detection surface 5211 of the calibration textile sample 521 can be combined with the previously obtained bacterial cell distribution map, significantly accelerating the online real-time detection and rapid imaging of bacteria on the entire detection surface 5211 of the calibration textile sample 521 by the imaging device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention.

[0035] Further, in order to enable the sample calibration system 50 to better simulate the antibacterial treatment of textiles by the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention and improve the detection accuracy, the calibrated textile sample 521 is prepared from a pretreated textile sample: First, the surface of the textile sample is depilated, for example, by burning or shearing treatment, to generate the detection surface 5211. The fiber fuzz on the surface of the textile sample will affect the imaging of the imaging device 51. By depilating the surface of the textile sample, the fiber fuzz can be removed to obtain the detection surface 5211. Then, the textile sample is sterilized, and then washed and dried. Sterilizing the textile sample (such as high-temperature steam sterilization) is to avoid the influence of the bacteria originally attached to the textile on the detection effect. The washing treatment is to remove the bacterial debris generated by sterilization and the ash generated by burning the surface of the textile sample or the cilia debris generated by shearing, so as to avoid its influence on the detection effect. Then, the dried textile sample is laid flat horizontally on the sample plate 522 with the detection surface 5211 of the textile sample facing upward, and the calibration bacterial solution is sprayed onto the detection surface 5211 of the textile sample. Then, the textile sample after spraying the calibration bacterial solution is placed in a room temperature environment and dried under negative pressure to obtain the calibrated textile sample 521. Drying under negative pressure at room temperature can remove the moisture of the calibrated textile sample 521 and enable the bacteria to adhere to the surface of the calibrated textile sample 521 without inactivating the bacteria adhering to the surface of the calibrated textile sample 521. Preferably, the textile sample used to prepare the calibrated textile sample 521 is a hotel textile sample, such as a towel, a bath towel or a pillowcase, etc., so as to better simulate the antibacterial treatment process of hotel textile products when antibacterial treatment is carried out on hotel textile products. Correspondingly, the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention can determine the antibacterial treatment effect of the textiles placed in the magnetic flux space 201 and to be treated by detecting the number and morphology of bacteria on the detection surface 5211 of the calibrated textile sample 521, thereby providing a judgment basis for the operator to accurately control the antibacterial operation of the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention.

[0036] It should be noted that the bacteria in the above-mentioned calibrated bacterial solution are all live bacteria stained with fluorescence. The staining agent used is a fluorescent dye that can stain live bacteria. For example, SYTO 9 green fluorescent dye. The excitation wavelength of SYTO 9 is 480 nm, and the emission wavelength is 500 nm. It has cell membrane permeability and can bind to DNA and RNA with high affinity, showing a significantly enhanced fluorescence signal after binding. The staining agent used can also be other fluorescent dyes that can stain live bacteria, such as red fluorescent dye or blue fluorescent dye. There are many existing dyes that can stain bacteria. For example, dyes such as methylene blue, crystal violet, basic fuchsin, safranin, and malachite green can stain bacteria by utilizing the charged characteristics on the surface of bacteria; crystal violet and iodine solution can stain Gram-positive bacteria and Gram-negative bacteria differently by using the different biochemical properties on the cell walls of different bacteria; dyes such as aniline stain different bacteria differently and can be used to identify the types of bacteria; safranin and malachite green can stain bacterial spores. However, the bacterial staining agent used in the calibrated bacterial solution of the present invention is a fluorescent dye. More specifically, it is a green, red or blue fluorescent dye, such as SYTO 9 green fluorescent dye. The light in the magnetic flux space 201 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the embodiment of the present invention is relatively dim. After the bacteria are stained with the fluorescent dye, they emit fluorescence of a specific wavelength (for example, light with a wavelength of 500 nm) under the excitation of the excitation light (for example, light with a wavelength of 480 nm), which can be used to locate the position of the bacteria on the detection surface 5211 of the calibrated textile sample 521, facilitating the rapid imaging and microscopic imaging of the imaging device 51. Several other common bacterial staining methods cannot better image under low-light conditions and help quickly locate the position of the bacteria on the detection surface 5211 of the calibrated textile sample 521.

[0037] Specifically, the calibrated bacterial solution of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the embodiment of the present invention is prepared by a standard fluorescent dye staining method. The following takes SYTO 9 fluorescent dye as an example to illustrate the fluorescent staining of bacteria by the fluorescent dye standard staining method: Separate the bacteria grown to the late logarithmic growth phase and resuspend the separated bacteria with 0.85% NaCl solution; Incubate at room temperature for 1 hour and invert and mix evenly every 15 minutes; Centrifuge the bacterial suspension after incubation at room temperature at 10000×g for 10 minutes, discard the supernatant to obtain a bacterial precipitate; Resuspend the bacterial precipitate with 0.85% NaCl solution to obtain a bacterial resuspension; Sample and detect the bacterial density of the bacterial resuspension; Adjust the bacterial suspension with 0.85% NaCl solution to make its density 10 6 bacteria / mL; Add an appropriate amount of SYTO 9 solution to the bacterial resuspension, mix evenly, and incubate in the dark at room temperature for 15 minutes to obtain a stained bacterial suspension; Centrifuge the stained bacterial suspension in the dark at room temperature, discard the supernatant, and resuspend with 0.85% NaCl solution; Finally, adjust the bacterial suspension with 0.85% NaCl solution to make its density 102 bacteria / mL, shake well to obtain the calibration bacterial solution of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the embodiment of the present invention. It should be noted that in order to ensure that enough single-distributed bacteria can be formed on the detection surface 5211 of the calibration textile sample 521, the density of the stained bacterial suspension should not be higher than 10 2 bacteria / mL. It can be understood that the reagents used for staining the calibration bacterial solution of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the embodiment of the present invention are all well-known to those skilled in the art. For example, the SYTO 9 solution is a standard SYTO 9 reagent produced by GLPBIO. Generally, different types of textiles are attached with different types of bacteria. For example, the bacteria attached to ordinary close-fitting clothes mainly include Staphylococcus aureus, Escherichia coli, Candida albicans and other bacteria; the bacteria attached to hotel towels, bath towels and bedding mainly include Staphylococcus aureus, Escherichia coli, hemolytic streptococcus and molds; the bacteria attached to newly bought clothes mainly include Escherichia coli, Staphylococcus aureus and streptococcus. Therefore, when the textile to be treated in the magnetic flux space 201 is close-fitting clothing, the calibration bacterial solution of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the embodiment of the present invention should at least contain Staphylococcus aureus, Escherichia coli and Candida albicans; when the textile to be treated in the magnetic flux space 201 is hotel textiles, the calibration bacterial solution of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the embodiment of the present invention should at least contain Staphylococcus aureus, Escherichia coli, hemolytic streptococcus and molds; when the textile to be treated in the magnetic flux space 201 is hotel textiles, the calibration bacterial solution of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the embodiment of the present invention should at least contain Escherichia coli, Staphylococcus aureus and streptococcus. Correspondingly, the calibration bacterial solution of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the embodiment of the present invention should at least contain Staphylococcus aureus and Escherichia coli, and the detection surface 5211 of the calibration textile sample 521 is at least attached with Staphylococcus aureus and Escherichia coli stained with live bacterial fluorescent dye. The data processing and control device 513 of the imaging device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the present invention can detect the type of bacteria on the detection surface 5211 of the calibration textile sample 521, the number of different types of bacteria, and the change in the number of bacteria at different detection times by identifying the number and morphology of bacteria at different detection times, and determine the antibacterial effect of the antibacterial treatment device on different types of bacteria, so as to provide a judgment basis for the operator to accurately control the antibacterial operation of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to the present invention.

[0038] As shown in the attached picture Figures 3 to 10 As shown, specifically, the camera device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulse magnetic field according to the embodiment of the present invention further includes a first fill light 514 and a second fill light 515, wherein the first fill light 514 is arranged adjacent to the fluorescent camera 511, and the second fill light 515 is arranged adjacent to the microscopic camera 512, wherein the first fill light 514 is arranged to emit excitation light according to the wavelength of the excitation light of the fluorescent dye (or the sensing light of the photosensitive element of the fluorescent camera 511), and the second fill light 515 is arranged to emit imaging light according to the wavelength of the sensing light of the photosensitive element of the microscopic camera 512. For example, when the fluorescent dye is SYTO 9, the first fill light 514 emits excitation light with a wavelength of 480nm; when the sensing light of the photosensitive element of the microscopic camera 512 is visible light, the second fill light 515 emits visible light. Preferably, the first fill light 514 and the second fill light 515 are both arranged to face the common imaging area.

[0039] As shown in the attached picture Figures 3 to 10 As shown, specifically, the camera device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulse magnetic field according to an embodiment of the present invention further includes a base 516, wherein the fluorescent camera 511, the microscopic camera 512, the first fill light 514 and the second fill light 515 are all arranged on the base 516 and are all arranged to face downward. Preferably, the base 516 is a telescopic base so that the fluorescent camera 511 and the microscopic camera 512 of the camera device 51 can be focused and a clearer image can be obtained.

[0040] As shown in the attached picture Figures 3 to 10 As shown, the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulse magnetic field according to an embodiment of the present invention further includes a guide rail 53, wherein the guide rail 53 is arranged on the inner side wall of the top 21 of the inner shell 20, and the base 516 is movably arranged on the guide rail 53, wherein the extension direction of the guide rail 53 is arranged to be parallel to the direction of the magnetic lines of force formed by the magnetic coil 41.

[0041] As shown in the attached picture Figures 3 to 10As shown, the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to an embodiment of the present invention further includes a magnetic shielding cylinder 54, and the magnetic shielding cylinder 54 forms a magnetic shielding space 540. The imaging device 51 of the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field of the present invention is disposed in the magnetic shielding space 540. It can be understood that the magnetic shielding cylinder 54 is made of a high-permeability material, such as iron, nickel, cobalt, etc., or an alloy thereof, so that the magnetic field bypasses the magnetic shielding space 540 and prevents the imaging device 51 from being affected by the magnetic field in the magnetic flux space 201. Preferably, the magnetic shielding cylinder 54 is disposed around the imaging device 51, and the magnetic shielding cylinder 54 is fixed on the outside of the imaging device 51.

[0042] As shown in the accompanying drawings Figures 3 to 10 As shown, the sample calibration system 50 of the textile antibacterial treatment device based on low-frequency pulsed magnetic field according to an embodiment of the present invention further includes a sample calibration chamber 55 disposed in the magnetic flux space 201. The sample calibration chamber 55 includes a chamber body 551 and a chamber door 552. The chamber body 551 forms a chamber 5510, and the chamber door 552 is disposed at the chamber door 5511 of the chamber 5510 for closing the chamber door 5511. The magnetic shielding cylinder 54 is disposed in the chamber 5510, and the imaging device 51 is disposed in the magnetic shielding space 540. Accordingly, the magnetic shielding cylinder 54 ensures that the magnetic field bypasses the magnetic shielding space 540 and prevents the imaging device 51 from being affected by the magnetic field in the magnetic flux space 201. The sample calibration chamber 55 ensures that the calibrated textile sample 521 therein can be effectively treated by the low-frequency pulsed magnetic field in the magnetic flux space 201. Preferably, both the sample calibration chamber 55 and the sample plate 522 are made of non-magnetic materials, such as plastics, and their thickness is not greater than 2 cm to ensure that the magnetic field lines in the magnetic flux space 201 can pass through the sample calibration chamber 55 and the sample plate 522. More preferably, both the sample calibration chamber 55 and the sample plate 522 are made of light-impermeable materials, such as light-impermeable plastics (or blackbody plastics), so that the chamber 5510 of the sample calibration chamber 55 forms a light-shielding space to prevent the light in the magnetic flux space 201 from affecting the imaging of the imaging device 51. Preferably, the chamber body 551 of the sample calibration chamber 55 further forms a fixing groove 5512, and the shape and size of the fixing groove 5512 are designed according to the shape and size of the sample plate 522 so that the fixing groove 5512 can accommodate the sample plate 522 and fix the sample plate 522 in place. As shown in the accompanying drawings Figures 3 to 11As shown, the antibacterial treatment of textiles based on low-frequency pulsed magnetic field according to the embodiments of the present invention for the textiles placed in the magnetic flux space 201 is as follows: After placing the textiles to be treated in the magnetic flux space 201, putting the sample plate 522 into the chamber 5510 of the sample calibration chamber 55, and closing the chamber door 551, first, the data processing and control device 513 of the sample calibration system 50 remotely or locally starts the micro camera 512 and the fluorescence camera 511 of the imaging device 51 of the sample calibration system 50. Combining with the bacterial cell distribution map of the entire detection surface 5211 of the calibration textile sample 521, fluorescence imaging and microscopic imaging are performed on the bacteria on the detection surface 5211 of the calibration textile sample 521 horizontally laid on the sample plate 522. Then, at least 10 clearly imaged single-distributed bacteria are randomly selected as the calibration bacterial cells, and a first real-time detection image of the calibration bacterial cells is generated according to the position distribution of the calibration bacterial cells. It can be understood that all the at least 10 clearly imaged single-distributed bacteria randomly selected should be distributed in the common imaging area of the micro camera 512 and the fluorescence camera 511, so that the micro camera 512 and the fluorescence camera 511 can respectively image the at least 10 clearly imaged single-distributed bacteria (or their positions). After the data processing and control device 513 obtains the first real-time detection image through the imaging device 51, the antibacterial device can be immediately powered on, and power is supplied to the magnetic coil 41 so that the magnetic coil 41 can generate a preset pulsed magnetic field and perform antibacterial treatment on the textiles, where the preset pulsed magnetic field has a preset pulse width (0 - 100 μs), pulse frequency (0 - 1000 Hz), and electric field intensity (10 - 50 kV / cm). Every 5 - 10 minutes of antibacterial treatment (the general antibacterial treatment time for textiles is 180 minutes), the data processing and control device 513 of the sample calibration system 50 starts the micro camera 512 and the fluorescence camera 511 of the imaging device 51. According to the first real-time detection image, the number and morphology of the bacterial cells at the positions of the at least 10 clearly imaged single-distributed bacteria randomly selected are detected, and the proliferation and death conditions of the at least 10 clearly imaged single-distributed bacteria randomly selected are determined, so as to determine the antibacterial treatment effect of the textiles placed in the magnetic flux space 201 and being treated.If, during the antibacterial treatment process, all of the bacteria cells located at the positions of at least 10 randomly selected single, clearly imaged bacteria are lysed, then the antibacterial effect of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the present invention is good, and the operator is allowed to end the antibacterial treatment in advance to reduce the time cost and save energy; if, during and / or after the antibacterial treatment process, the number of bacteria cells located at the positions of at least 10 randomly selected single, clearly imaged bacteria remains unchanged or even partially lyses, then the antibacterial effect of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the present invention is good; if the number of bacteria cells located at the positions of at least 10 randomly selected single, clearly imaged bacteria increases significantly (for example, the number of bacteria cells at the positions of at least 10 randomly selected single, clearly imaged bacteria increases by 10% or more, each increasing by at least 1), then the antibacterial effect of the textile antibacterial treatment device based on a low-frequency pulsed magnetic field according to the present invention is poor, and it is necessary to timely change at least one variable of the pulse width, pulse frequency, and electric field strength of the preset pulsed magnetic field and re-perform antibacterial treatment on the textile in the magnetic flux space 201.

[0043] Correspondingly, according to an embodiment of the present invention, the present invention further provides a textile antibacterial treatment method based on a low-frequency pulsed magnetic field, which includes the following steps:

[0044] Place the textile to be treated in the magnetic flux space 201, place the sample plate 522 into the chamber 5510 of the sample calibration chamber 55, and close the chamber door 551;

[0045] Through the microscopic camera 512 and the fluorescence camera 511 of the imaging device 51 of the sample calibration system 50, under the guidance of the bacterial cell distribution map of the detection surface 5211 of the calibrated textile sample 521, perform fluorescence imaging and microscopic imaging on the bacteria on the detection surface 5211 of the calibrated textile sample 521 horizontally laid on the sample plate 522;

[0046] Randomly select at least 10 single, clearly imaged bacteria as the calibrated bacteria cells, and generate a first real-time detection image of the calibrated bacteria cells according to the position distribution of the calibrated bacteria cells;

[0047] Immediately start the antibacterial device, supply power to the magnetic coil 41 of the antibacterial device, so that the magnetic coil 41 can generate a preset pulsed magnetic field and perform antibacterial treatment on the textile;

[0048] For every 5 - 10 minutes of antibacterial treatment, through the microscopic camera 512 and the fluorescence camera 511 of the imaging device 51 of the sample calibration system 50, referring to the first real - time detection image, the number and morphology of the bacterial cells at the locations of at least 10 randomly selected single - distributed bacteria with clear imaging are detected again, so as to obtain the Nth real - time detection image; and

[0049] Compare the number and morphology of the bacterial cells at the locations of at least 10 randomly selected single - distributed bacteria with clear imaging in the first real - time detection image and the Nth real - time detection image. If the number of the bacterial cells at the locations of at least 10 randomly selected single - distributed bacteria with clear imaging increases, at least one variable of the pulse width, pulse frequency, and electric field strength of the preset pulsed magnetic field is changed and the textile in the magnetic flux space 201 is re - treated with antibacterial treatment. It can be understood that the physiological states of different types of bacteria are different under different environmental temperatures and humidities, and their response degrees to pulsed magnetic fields with different pulse widths, pulse frequencies, and electric field strengths are also different. When the number of the bacterial cells at the locations of at least 10 randomly selected single - distributed bacteria with clear imaging increases, the operator can change at least one variable of the pulse width, pulse frequency, and electric field strength of the preset pulsed magnetic field and re - treat the textile in the magnetic flux space 201 with antibacterial treatment to improve the antibacterial effect.

[0050] It can be understood that the first and / or second in this article are only used for naming different components (or elements) of the present invention and to distinguish between different components, elements, and structures of the present invention. Unless otherwise specified, they do not have the meaning of order or quantity by themselves.

[0051] Those skilled in the art will understand that the embodiments of the present invention shown in the drawings and described above are only examples of the present invention rather than limitations.

[0052] From this, it can be seen that the object of the present invention can be fully and effectively achieved. The embodiments used to explain the function and structural principle of the present invention have been fully described and explained, and the present invention is not limited by changes based on the principles of these embodiments. Therefore, the present invention includes all modifications covered within the scope and spirit of the appended claims.

Claims

1. A textile antibacterial treatment method based on low-frequency pulsed magnetic field, characterized in that, Including: Placing the textile to be processed in the magnetic flux space, putting the sample plate into the chamber of the sample calibration chamber of the textile antibacterial device, and closing the chamber door; Under the guidance of the bacterial cell distribution map of the detection surface of the calibrated textile sample, performing fluorescence imaging and microscopic imaging on the bacteria on the detection surface of the calibrated textile sample horizontally laid on the sample plate through the microscopic camera and the fluorescence camera of the imaging device of the sample calibration system; Randomly selecting at least 10 clearly imaged single-distributed bacteria as the calibrated bacterial cells and generating a first real-time detection image of the calibrated bacterial cells according to the position distribution of the calibrated bacterial cells; Starting the antibacterial device, supplying power to the magnetic coil of the antibacterial device so that the magnetic coil can generate a preset pulsed magnetic field and perform antibacterial treatment on the textile; Every 5 - 10 minutes of antibacterial treatment, through the microscopic camera and the fluorescence camera of the imaging device of the sample calibration system, detecting again the quantity and morphology of the bacterial cells at the positions where the at least 10 clearly imaged single-distributed bacteria randomly selected are located, so as to obtain the Nth real-time detection image; And Comparing the quantity and morphology of the bacterial cells at the positions where the at least 10 clearly imaged single-distributed bacteria randomly selected in the first real-time detection image and the Nth real-time detection image are located. If the quantity of the bacterial cells at the positions where the at least 10 clearly imaged single-distributed bacteria randomly selected is increased, changing at least one variable of the pulse width, pulse frequency and electric field intensity of the preset pulsed magnetic field and re-performing antibacterial treatment on the textile in the magnetic flux space.

2. The textile antibacterial treatment method based on low-frequency pulsed magnetic field according to claim 1, wherein At least Staphylococcus aureus and Escherichia coli stained with live bacteria fluorescent dye are attached to the detection surface of the calibrated textile sample.

3. The textile antibacterial treatment method based on low-frequency pulsed magnetic field according to claim 1, characterized in that, Both the sample calibration chamber and the sample plate are made of non-magnetic materials, and the non-magnetic materials are light-impermeable materials.

4. The textile antibacterial treatment method based on low-frequency pulsed magnetic field according to claim 3, characterized in that, The imaging device is arranged in the magnetic shielding space of the magnetic shielding cylinder, and the magnetic shielding cylinder is arranged in the chamber.

5. The textile antibacterial treatment method based on low-frequency pulsed magnetic field according to claim 1, characterized in that, The calibrated textile sample is prepared through the following steps: Performing defurring treatment on the surface of the textile sample to generate the detection surface; Performing sterilization treatment on the textile sample, and cleaning and drying; Horizontally laying the dried textile sample on the sample plate with the detection surface of the textile sample facing upward; Spraying calibration bacterial liquid on the detection surface of the textile sample, wherein the calibration bacterial liquid contains at least Escherichia coli and Staphylococcus aureus; and Placing the textile sample sprayed with calibration bacterial liquid in a room temperature environment and drying it under negative pressure to obtain the calibrated textile sample.

6. The textile antibacterial treatment method based on low-frequency pulsed magnetic field according to claim 5, wherein The density of the stained bacterial suspension is not higher than 10 2 bacteria / mL.

7. The textile antibacterial treatment method based on low-frequency pulsed magnetic field according to claim 5, characterized in that The fluorescent dye is a green fluorescent dye, a red fluorescent dye or a blue fluorescent dye.

8. The textile antibacterial treatment method based on low-frequency pulsed magnetic field according to claim 1, characterized in that, The highest frequency of the low-frequency pulsed magnetic field is not higher than 1000 Hertz.

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