Biological stem cell culture equipment

Abstract

The invention discloses biological stem cell culture equipment, and relates to the technical field of biological stem cell culture, the biological stem cell culture equipment comprises a machine body used for biological stem cell culture operation, and the machine body is provided with an operation window used for assisting culture operation; a control assembly for controlling in the culture process is arranged at the upper end of the machine body. In the process that the biological stem cell culture equipment is used for culturing biological stem cells and performing liquid change treatment, through mutual cooperation of the overturning and inclining assembly, the linkage assembly, the transmission assembly and other components, it can be guaranteed that culture liquid is stably taken, the pollution risk is reduced, and the purity of the stem cell culture environment is guaranteed; a good contact angle and distance can be always kept between the liquid suction port and the liquid level of the culture solution in the process that the liquid taking gun inclines and extracts the liquid, so that the phenomenon that the front end of the liquid taking gun is not completely inserted into the culture solution is avoided, and the influence on the culture solution in the liquid taking process is reduced while the liquid taking precision is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of biological stem cell culture, and particularly to a biological stem cell culture device. Background Art

[0002] The purposes of biological stem cell culture mainly include basic research, clinical application, drug research and development, etc. A biological stem cell culture device is an instrument used to culture stem cells and provide a suitable growth environment for stem cells to achieve operations such as the proliferation and differentiation of stem cells.

[0003] During the process of culturing biological stem cells with a biological stem cell culture device, some wastes will be produced during the metabolism of stem cells, such as carbon dioxide, lactic acid, ammonia, etc. If these metabolic wastes accumulate too much in the culture medium, it will change the pH value and osmotic pressure of the culture medium, inhibit the growth of stem cells, and even cause cell death. Therefore, when culturing biological stem cells with a biological stem cell culture device, a liquid exchange gun is needed to perform liquid exchange treatment on the culture medium. Liquid exchange can timely remove these metabolic wastes and maintain the stability of the culture medium environment.

[0004] Stem cells usually grow adherently on the bottom of the culture flask. To avoid the liquid extraction gun being inserted too deep and directly contacting the cells, resulting in the cells falling off the culture surface or causing physical damage to the cells, affecting the cell activity and normal growth. During the liquid exchange operation, the liquid extraction gun is inserted shallowly into the culture medium. With the liquid extraction during liquid exchange, the front end of the liquid extraction gun is not completely inserted into the culture medium, resulting in the actual liquid extraction volume being less than the set volume. And because the front end of the liquid extraction gun is not completely inserted into the culture medium, air bubbles may be generated under the action of pressure when sucking the liquid. On the one hand, it causes waste of the culture medium, and on the other hand, it increases the risk of contamination of the operation area. For this reason, we propose a biological stem cell culture device. Summary of the Invention

[0005] The purpose of the present invention is to provide a biological stem cell culture device to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A biological stem cell culture device, including a body for biological stem cell culture operations, an operation window for assisting culture operations is opened on the body, and a control component for controlling during the culture process is arranged at the upper end of the body; further includes:

[0007] A carrier platform is arranged inside the machine body. A positioning and placing cylinder for sleeving and positioning a culture bottle is fixedly centered at the upper end of the carrier platform. An overturning and tilting assembly is arranged inside the machine body for assisting the carrier platform and the culture bottle placed on the carrier platform to overturn and tilt. An installation plate is arranged inside the machine body. A support and installation assembly is arranged inside the inner side of the machine body for assisting in supporting and installing the installation plate. A liquid extraction gun for changing the liquid in the culture bottle during the culture process is installed on the installation plate through an installation assembly. The installation plate and the liquid extraction gun installed on the installation plate are both arranged in an inclined state. A moving assembly for moving the installed liquid extraction gun is arranged on the installation plate. A linkage assembly for assisting in linkage is arranged between the installation plate and the carrier platform. A transmission assembly for transmitting the liquid extraction gun during the linkage process of the installation plate is arranged on the support and installation assembly.

[0008] Preferably, the installation assembly includes an installation sleeve for placing the liquid extraction gun. A threaded hole is opened on the installation sleeve, and a threaded pin for abutting and limiting the placed liquid extraction gun is threadedly engaged on the threaded hole.

[0009] Preferably, the moving assembly includes an installation groove opened on the installation plate. A connecting plate is arranged inside the installation groove. The installation sleeve is fixed on the connecting plate. A threaded sleeve is fixed on the connecting plate. A threaded rod is threadedly engaged on the threaded sleeve. The threaded rod is rotatably connected inside the installation groove. A first motor for driving the threaded rod is installed on the installation plate. Two sliding rods are slidably connected to the connecting plate, and the two sliding rods are symmetrically arranged on both sides of the threaded sleeve. The sliding rods are fixed inside the installation groove.

[0010] Preferably, the support and installation assembly includes a sector plate arranged inside the machine body. The sector plate and the machine body are fixedly connected by a plurality of connecting rods. The installation plate is arranged on the front side of the sector plate, and a sliding assembly for assisting in slidably connecting the installation plate is arranged between the installation plate and the sector plate.

[0011] Preferably, the sliding assembly includes a sector groove opened on the sector plate. Two arc-shaped guide rods arranged symmetrically are fixed inside the sector groove. A connecting block is slidably connected to the two arc-shaped guide rods. The two connecting blocks are fixed to the back surface of the installation plate.

[0012] Preferably, springs are sleeved on the outer sides of the two arc-shaped guide rods. The two ends of the springs are respectively connected to the inner wall of the sector groove and the connecting block.

[0013] Preferably, the linkage assembly includes a first linkage plate fixed to the upper end of the carrier platform. A second linkage plate for abutting and transmitting with the first linkage plate is fixed to the lower end of the installation plate.

[0014] Preferably, the transmission assembly includes a gear fixed on the threaded rod, and a sector rack for meshing transmission with the gear is fixed on the sector plate.

[0015] Preferably, the flipping and tilting assembly includes a U-shaped seat fixed inside the machine body. An installation shaft is rotatably connected to the U-shaped seat. Two installation blocks are fixed on the installation shaft. The two installation blocks are symmetrically fixed at the lower end of the carrier table. A second motor for driving the installation shaft is installed on the U-shaped seat.

[0016] Preferably, the sector groove and the arc-shaped guide rod are concentric with the installation shaft.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0018] During the process of culturing and changing the liquid of biological stem cells using the biological stem cell culture equipment of the present invention, through the mutual cooperation of components such as the flipping and tilting assembly, the linkage assembly, and the transmission assembly, it can not only ensure stable liquid extraction of the culture medium, reduce the risk of contamination, and ensure the purity of the stem cell culture environment, but also enable the liquid suction port of the liquid extraction gun to always maintain a good contact angle and distance with the liquid surface of the culture medium during the process of tilting and pumping liquid, avoiding the situation that the front end of the liquid extraction gun is not completely inserted into the culture medium, reducing the impact on the culture medium during the liquid extraction process while ensuring the liquid extraction accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic diagram of the overall external structure of the present invention;

[0020] Figure 2 is a schematic diagram of the internal structure of the equipment of the present invention;

[0021] Figure 3 is a schematic diagram of the positional relationship between the carrier table and the mounting plate of the present invention;

[0022] Figure 4 is a schematic diagram of the flipping and tilting assembly, the sliding assembly, and the support and mounting assembly of the present invention;

[0023] Figure 5 is a schematic diagram of the positional relationship between the mounting plate and the liquid extraction gun of the present invention;

[0024] Figure 6 is a schematic diagram of the mounting assembly, the moving assembly, and the transmission assembly of the present invention;

[0025] Figure 7 is a schematic diagram of the state before liquid extraction during the cell culture process of the present invention;

[0026] Figure 8 is a schematic diagram of the preliminary flipping and tilting state of the culture bottle during the cell culture process of the present invention;

[0027] Figure 9 Schematic diagram of the state when the liquid sampling gun is inserted into the culture flask during the cell culture process of the present invention;

[0028] Figure 10 Schematic diagram of the state when the culture flask is turned and tilted again during the cell culture process of the present invention.

[0029] In the figure: 101 - body; 102 - operation window; 103 - control component; 2 - bearing platform; 3 - positioning placement cylinder; 401 - U-shaped seat; 402 - mounting shaft; 403 - mounting block; 404 - second motor; 5 - mounting plate; 6 - liquid sampling gun; 701 - mounting sleeve; 702 - threaded hole; 703 - threaded pin; 801 - mounting groove; 802 - connecting plate; 803 - threaded sleeve; 804 - threaded rod; 805 - first motor; 806 - sliding rod; 901 - sector plate; 902 - connecting rod; 1001 - sector groove; 1002 - arc guide rod; 1003 - connecting block; 1004 - spring; 1101 - first linkage plate; 1102 - second linkage plate; 1201 - gear; 1202 - sector rack. Detailed implementation manners

[0030] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0031] Embodiment 1

[0032] Please refer to Figures 1 - 10 , a biological stem cell culture device shown in the figure, including a body 101 for biological stem cell culture operations. An operation window 102 for assisting culture operations is opened on the body 101, and a control component 103 for controlling during the culture process is arranged at the upper end of the body 101;

[0033] It should be noted here that: the body 101 is a conventional operation device in the field of cell culture technology, and its working principle and the settings of other internal components are regarded as prior art in this application and will not be elaborated herein;

[0034] It is worth noting here that: the control component 103 is mainly used to control the temperature, humidity, carbon dioxide concentration, etc. inside the body 101. As a conventional control operation component, it is regarded as prior art in this application and will not be elaborated herein;

[0035] It further includes:

[0036] The bearing platform 2 is arranged inside the machine body 101. A positioning and placing cylinder 3 for sleeving and positioning a culture bottle is fixedly centered at the upper end of the bearing platform 2. An overturning and tilting assembly for assisting the bearing platform 2 and the culture bottle placed on the bearing platform 2 to overturn and tilt is arranged inside the machine body 101. An installation plate 5 is arranged inside the machine body 101. A support and installation assembly for assisting the support and installation of the installation plate 5 is arranged on the inner side of the machine body 101. A liquid extraction gun 6 for changing the liquid of the culture bottle during the culture process is installed on the installation plate 5 through an installation assembly. The installation plate 5 and the liquid extraction gun 6 installed on the installation plate 5 are both arranged in an inclined state. A moving assembly for moving the installed liquid extraction gun 6 is arranged on the installation plate 5. A linkage assembly for assisting linkage is arranged between the installation plate 5 and the bearing platform 2. A transmission assembly for transmitting the liquid extraction gun 6 during the linkage process of the installation plate 5 is arranged on the support and installation assembly;

[0037] It should be noted here that during the process of culturing and changing the liquid of biological stem cells using a biological stem cell culture device, through the mutual cooperation of components such as the overturning and tilting assembly, the linkage assembly, and the transmission assembly, it can not only ensure stable liquid extraction of the culture medium, reduce the risk of contamination, and ensure the purity of the stem cell culture environment, but also enable the liquid extraction port of the liquid extraction gun 6 to always maintain a good contact angle and distance with the liquid surface of the culture medium during the process of tilting and pumping liquid, avoiding the situation that the front end of the liquid extraction gun 6 is not completely inserted into the culture medium, ensuring the accuracy of liquid extraction while reducing the impact on the culture medium during the liquid extraction process.

[0038] Preferably, the installation assembly includes an installation sleeve 701 for placing the liquid extraction gun 6. A threaded hole 702 is opened on the installation sleeve 701. A threaded pin 703 for abutting and limiting the placed liquid extraction gun 6 is threadedly engaged on the threaded hole 702;

[0039] It should be noted here that the liquid extraction gun 6 is sleeved and installed on the installation sleeve 701. After the installation is completed, the threaded pin 703 is rotated. During the rotation process, through the mutual meshing transmission between the threaded pin 703 and the threaded hole 702, one end of the threaded pin 703 abuts against the outer side of the liquid extraction gun 6 to install and fix the liquid extraction gun 6.

[0040] Preferably, the moving assembly includes an installation groove 801 opened on the installation plate 5. A connecting plate 802 is arranged inside the installation groove 801. The installation sleeve 701 is fixed on the connecting plate 802. A threaded sleeve 803 is fixed on the connecting plate 802. A threaded rod 804 is threadedly engaged on the threaded sleeve 803. The threaded rod 804 is rotatably connected inside the installation groove 801. A first motor 805 for driving the threaded rod 804 is installed on the installation plate 5. Two sliding rods 806 are slidably connected on the connecting plate 802, and the two sliding rods 806 are symmetrically arranged on both sides of the threaded sleeve 803. The sliding rods 806 are fixed inside the installation groove 801;

[0041] It should be noted here that: the first motor 805 drives the threaded rod 804 to rotate. During the rotation of the threaded rod 804, through the meshing transmission between the threaded rod 804 and the threaded sleeve 803 and the sliding guiding action of the sliding rod 806, the connecting plate 802 and the mounting sleeve 701 move after being stressed.

[0042] Preferably, the support and installation assembly includes a sector plate 901 arranged inside the machine body 101. The sector plate 901 is fixedly connected to the machine body 101 through multiple groups of connecting rods 902. The mounting plate 5 is arranged on the front side of the sector plate 901, and a sliding assembly for assisting the sliding connection of the mounting plate 5 is arranged between the mounting plate 5 and the sector plate 901;

[0043] It should be noted here that: through the connection between the sector plate 901, the connecting rods 902 and the sliding assembly, the support and installation of the sector plate 901 are assisted.

[0044] Preferably, the sliding assembly includes a sector groove 1001 opened on the sector plate 901. Two symmetrically arranged arc-shaped guide rods 1002 are fixed inside the sector groove 1001. A connecting block 1003 is slidably connected to the two arc-shaped guide rods 1002, and the two connecting blocks 1003 are fixed to the back of the mounting plate 5;

[0045] It should be noted here that: through the sector groove 1001, the arc-shaped guide rods 1002 and the connecting block 1003, the movement of the mounting plate 5 after being stressed is slidably guided.

[0046] Preferably, springs 1004 are sleeved on the outer sides of the two arc-shaped guide rods 1002. The two ends of the springs 1004 are respectively connected to the inner wall of the sector groove 1001 and the connecting block 1003;

[0047] It should be noted here that: through the elastic connection of the springs 1004, it is convenient to assist the reset of the mounting plate 5 after movement.

[0048] Preferably, the linkage assembly includes a first linkage plate 1101 fixed to the upper end of the bearing platform 2. A second linkage plate 1102 for abutting and transmitting with the first linkage plate 1101 is fixed to the lower end of the mounting plate 5.

[0049] Preferably, the transmission assembly includes a gear 1201 fixed on the threaded rod 804, and a sector rack 1202 for meshing and transmitting with the gear 1201 is fixed on the sector plate 901;

[0050] It should be noted here that during the process of moving the mounting plate 5 on the sector plate 901 through linkage, the meshing transmission between the gear 1201 and the sector rack 1202 drives the threaded rod 804 on the moving component to rotate. During the rotation of the threaded rod 804, through the transmission on the moving component, the connecting plate 802 and the liquid extraction gun 6 fixedly installed on the mounting sleeve 701 are forced to move towards the interior of the culture solution in the culture flask.

[0051] Preferably, the flipping and tilting component includes a U-shaped seat 401 fixed inside the machine body 101. A mounting shaft 402 is rotatably connected to the U-shaped seat 401. Two mounting blocks 403 are fixed on the mounting shaft 402. The two mounting blocks 403 are symmetrically fixed to the lower end of the carrier table 2. A second motor 404 for driving the mounting shaft 402 is installed on the U-shaped seat 401;

[0052] It should be noted here that through the second motor 404, the mounting shaft 402 is driven to rotate. During the rotation of the mounting shaft 402, through the connection of the mounting block 403, the carrier table 2 is driven to perform a flipping and tilting movement.

[0053] Preferably, the sector groove 1001 and the arc-shaped guide rod 1002 are concentric with the mounting shaft 402;

[0054] It should be noted here that through the concentric arrangement of the sector groove 1001 and the arc-shaped guide rod 1002 with the mounting shaft 402, when the sector groove 1001 and the arc-shaped guide rod 1002 slide and guide the mounting plate 5, the stressed mounting plate 5 slides with the axis of the mounting shaft 402 as the center.

[0055] In this solution: A biological stem cell culture device includes the following steps:

[0056] During the process of using the biological stem cell culture device to perform a culture operation on biological stem cells, the biological stem cell culture solution is placed inside the culture flask. After being transported and placed, the culture flask is sleeved and installed inside the positioning placement cylinder 3 of the carrier table 2 and is in a vertically upward state. After the placement is completed, the control component 103 on the machine body 101 controls the temperature, humidity, carbon dioxide concentration, etc. of the internal culture environment during the culture process to achieve the culture operation of biological stem cells;

[0057] During the culture process, the mounting plate 5, the liquid extraction gun 6 and the sector plate 901 are arranged in an inclined state above the culture flask (see Figure 7) to reduce the probability of contaminants such as dust and microorganisms on the mounting plate 5, liquid extraction gun 6, and sector plate 901 directly falling into the culture bottle, reduce the pollution risk, and ensure the purity of the stem cell culture environment. During the culture process, to reduce the impact of excessive accumulation of metabolic waste in the culture medium during cell culture, the culture medium inside the culture bottle is replaced. During the liquid replacement process, the carrier platform 2 and the culture bottle placed on the carrier platform 2 are tilted and rotated by the flipping and tilting assembly. During the tilting and rotating process, the first linkage plate 1101 on the carrier platform 2 abuts against the second linkage plate 1102 on the mounting plate 5 (see Figure 8 ). After abutting, the initial flipping and tilting rotation of the carrier platform 2 and the culture bottle placed on the carrier platform 2 is stopped. At this time, through the moving assembly, the connecting plate 802 and the liquid extraction gun 6 fixedly installed on the mounting sleeve 701 move towards the inside of the culture bottle. During the movement, the front end of the liquid extraction gun 6 moves and inserts into the inside of the culture medium in the culture bottle (see Figure 9 ). And during the insertion process, to avoid affecting the stem cells at the bottom of the culture medium, through the precise movement control of the moving assembly, the insertion depth of the liquid extraction gun 6 is relatively low. After the liquid extraction gun 6 is inserted, the culture medium is extracted and replaced through the driving action of the liquid extraction gun 6;

[0058] And when the liquid extraction gun 6 is inserted and the culture medium is extracted and replaced, through the flipping and tilting assembly, the carrier platform 2 and the culture bottle placed on the carrier platform 2 are driven to perform further tilting rotation, increasing the tilting angle of the culture bottle. As the liquid extraction of the culture medium progresses, the amount of the culture medium gradually decreases. Increasing the tilting angle of the culture bottle can make the remaining culture medium concentrate on one side of the bottom of the culture bottle, making it easier for the liquid extraction gun 6 to contact these liquids and suck as much culture medium as possible. And during the further flipping and tilting operation of the carrier platform 2, because the first linkage plate 1101 on the carrier platform 2 abuts against the second linkage plate 1102 on the mounting plate 5, through the abutting transmission action between the first linkage plate 1101 and the second linkage plate 1102 and the sliding connection action of the sliding assembly on the mounting plate 5, the carrier platform 2 and the culture bottle push the mounting plate 5 and the liquid extraction gun 6 on the mounting plate 5 to move synchronously on the sector plate 901 during the second flipping and tilting. Through the synchronous movement of the culture bottle and the liquid extraction gun 6, it is avoided that the liquid extraction gun 6 moves horizontally inside the culture bottle and affects the stability of the internal culture medium, and the synchronous movement of the culture bottle and the liquid extraction gun 6 avoids interference between the liquid extraction gun 6 and the culture bottle during the liquid extraction process and affects the normal liquid extraction operation;

[0059] During the process of driving the mounting plate 5 to move on the sector plate 901 through linkage, the rotation of the threaded rod 804 on the moving assembly is driven by the meshing transmission between the gear 1201 and the sector rack 1202. During the rotation of the threaded rod 804, through the transmission on the moving assembly, the liquid extraction gun 6 fixedly installed on the connecting plate 802 and the mounting sleeve 701 is forced to move towards the inside of the culture solution in the culture flask. Through the further insertion and movement of the liquid extraction gun 6 on the culture solution, the liquid suction port of the liquid extraction gun 6 always maintains a good contact angle and distance with the liquid level of the culture solution during the process of tilting and pumping liquid, avoiding the situation that the front end of the liquid extraction gun 6 is not fully inserted into the culture solution, ensuring the accuracy of liquid extraction while reducing the impact on the culture solution during the liquid extraction process.

[0060] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not explicitly listed, or further includes elements inherent to such process, method, article or device.

[0061] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological stem cell culture device, comprising: A body (101) for biological stem cell culture operations, an operation window (102) for assisting culture operations is provided on the body (101), and a control component (103) for controlling during the culture process is provided at the upper end of the body (101); It is characterized in that it further comprises: A carrier table (2) disposed inside the body (101), a positioning placement cylinder (3) for sleeving and positioning a culture bottle is fixedly centered at the upper end of the carrier table (2), a flipping and tilting component for assisting the carrier table (2) and the culture bottle on the carrier table (2) to flip and tilt after positioning is provided inside the body (101), a mounting plate (5) is provided inside the body (101), a support and mounting component for assisting the support and installation of the mounting plate (5) is provided on the inner side of the body (101), a liquid extraction gun (6) for performing liquid replacement operations on the culture bottle during the culture process is mounted on the mounting plate (5) through a mounting component, the mounting plate (5) and the liquid extraction gun (6) mounted on the mounting plate (5) are both disposed in an inclined state, a moving component for moving the mounted liquid extraction gun (6) is provided on the mounting plate (5), a linkage component for assisting linkage is provided between the mounting plate (5) and the carrier table (2), and a transmission component for transmitting the liquid extraction gun (6) during the linkage of the mounting plate (5) is provided on the support and mounting component.

2. The biological stem cell culture device according to claim 1, characterized in that: The mounting component includes a mounting sleeve (701) for placing the liquid extraction gun (6), a threaded hole (702) is provided on the mounting sleeve (701), and a threaded pin (703) for abutting and limiting the placed liquid extraction gun (6) is threadedly engaged in the threaded hole (702).

3. The biological stem cell culture device according to claim 2, characterized in that: The moving component includes a mounting groove (801) provided on the mounting plate (5), a connecting plate (802) is provided inside the mounting groove (801), the mounting sleeve (701) is fixed to the connecting plate (802), a threaded sleeve (803) is fixed to the connecting plate (802), a threaded rod (804) is threadedly engaged in the threaded sleeve (803), the threaded rod (804) is rotatably connected inside the mounting groove (801), a first motor (805) for driving the threaded rod (804) is mounted on the mounting plate (5), two groups of sliding rods (806) are slidably connected to the connecting plate (802), and the two groups of sliding rods (806) are symmetrically disposed on both sides of the threaded sleeve (803), and the sliding rods (806) are fixed inside the mounting groove (801).

4. The biological stem cell culture device according to claim 3, wherein: The support and mounting component includes a sector plate (901) disposed inside the body (101), the sector plate (901) is fixedly connected to the body (101) through a plurality of connecting rods (902), the mounting plate (5) is disposed on the front side of the sector plate (901), and a sliding component for assisting the sliding connection of the mounting plate (5) is provided between the mounting plate (5) and the sector plate (901).

5. The biological stem cell culture device according to claim 4, wherein: The sliding component includes a sector groove (1001) formed in the sector plate (901). Two groups of arc-shaped guide rods (1002) arranged symmetrically are fixed inside the sector groove (1001). A connecting block (1003) is slidably connected to the two groups of arc-shaped guide rods (1002), and the two groups of connecting blocks (1003) are fixed to the back surface of the mounting plate (5).

6. The biological stem cell culture device according to claim 5, wherein: Springs (1004) are sleeved on the outer sides of the two groups of arc-shaped guide rods (1002), and the two ends of each spring (1004) are respectively connected to the inner wall of the sector groove (1001) and the connecting block (1003).

7. A biological stem cell culture device according to claim 5, characterized in that: The linkage component includes a first linkage plate (1101) fixed to the upper end of the carrier (2). A second linkage plate (1102) for abutting and driving the first linkage plate (1101) is fixed to the lower end of the mounting plate (5).

8. A biological stem cell culture device according to claim 5, characterized in that: The transmission component includes a gear (1201) fixed to the threaded rod (804). A sector rack (1202) for meshing and driving with the gear (1201) is fixed to the sector plate (901).

9. The biological stem cell culture device according to claim 5, characterized in that: The flipping and tilting component includes a U-shaped seat (401) fixed inside the machine body (101). A mounting shaft (402) is rotatably connected to the U-shaped seat (401). Two mounting blocks (403) are fixed to the mounting shaft (402), and the two groups of mounting blocks (403) are symmetrically fixed to the lower end of the carrier (2). A second motor (404) for driving the mounting shaft (402) is installed on the U-shaped seat (401).

10. A biological stem cell culture device according to claim 9, characterized in that: The sector groove (1001) and the arc-shaped guide rod (1002) are concentric with the mounting shaft (402).

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