Liquid path system of full-automatic electrochemiluminescence immunoassay analyzer

Abstract

The application discloses a liquid path system of a full-automatic electrochemiluminescence immunoassay analyzer, belongs to the field of chemiluminescence immunoassay, and is characterized in that a liquid supply tank is in communication with a first centrifugal pump, part of liquid drawn by the first centrifugal pump forms system liquid used for cleaning, part of liquid drawn by the first centrifugal pump is deaerated by a deaerating device to form low-pressure deaerated water, and the gear pump pressurizes part of the low-pressure deaerated water to form high-pressure deaerated water; the high-pressure deaerated water flows to the inner wall of a sample adding needle and a reagent needle for cleaning; system liquid flows into a sample adding needle cleaning station, a reagent needle cleaning station and a needle group cleaning station to clean the outer wall of the needles; cleaning liquid in a cleaning liquid bottle flows to a sample adding needle cleaning liquid storage station and a reagent needle cleaning liquid storage station to clean the inner wall of the needles; the low-pressure deaerated water flows to a buffer control module to control the flow direction of buffer in a buffer bottle to a dispensing needle and a liquid suction needle; and the low-pressure deaerated water flows to a measuring pool and a liquid suction needle; through the above design, the liquid path system has high precision, good reliability and high repeatability.

Description

Technical Field

[0001] This invention relates to the field of chemiluminescence immunoassay, and in particular to the liquid circuit system of a fully automated electrochemiluminescence immunoassay analyzer. Background Technology

[0002] Luminescent immunoassay is an analytical method in which a sample is mixed with a variety of reagents containing magnetic particles and enzyme labels to induce an antigen-antibody reaction, a chemiluminescent substrate is added, and immunoassay is performed based on the luminescence intensity.

[0003] Existing fully automated chemiluminescence immunoassay analyzers typically use a syringe to sequentially and quantitatively transfer and mix samples and reagents, with the syringe being cleaned with a cleaning solution after each sample addition. The vast majority of dark sample detection failures during the operation of chemiluminescence assay instruments are attributed to malfunctions in the liquid path system. These malfunctions primarily include poor quantitative accuracy of the liquid path module, low reliability of individual modules within the system, and poor repeatability of each module during the testing process. Summary of the Invention

[0004] In order to overcome the shortcomings of the prior art, one of the objectives of this invention is to provide a liquid circuit system for a fully automated electrochemiluminescence immunoassay analyzer with high precision, high reliability and high repeatability.

[0005] One of the objectives of this invention is achieved through the following technical solution:

[0006] The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer includes a liquid supply module, a sample loading module, a reagent loading module, a cleaning and separation module, and a detection module.

[0007] The liquid supply module includes a liquid supply tank, a first centrifugal pump, a degassing device, and a gear pump. The liquid supply tank is connected to the first centrifugal pump, which draws liquid from the liquid supply tank. A portion of the liquid drawn by the first centrifugal pump forms a system liquid for cleaning. The degassing device is connected to the first centrifugal pump, which degasses a portion of the liquid drawn by the first centrifugal pump to form low-pressure degassed water. The gear pump is connected to the degassing device, which pressurizes a portion of the low-pressure degassed water to form high-pressure degassed water.

[0008] The sample loading module includes a loading needle, a loading needle cleaning station, a cleaning solution bottle, and a loading needle cleaning solution storage station. The loading needle is connected to the gear pump, and the high-pressure degassed water flows to the loading needle to clean the inner wall of the loading needle. The loading needle cleaning station is connected to the first centrifugal pump, and the system liquid flows into the loading needle cleaning station to clean the outer wall of the loading needle. The cleaning solution bottle is connected to the loading needle cleaning solution storage station, and the cleaning solution in the cleaning solution bottle flows to the loading needle cleaning solution storage station to clean the reagents on the inner wall of the loading needle.

[0009] The reagent dispensing module includes a reagent needle, a reagent needle cleaning station, and a reagent needle cleaning solution storage station. The reagent needle is connected to the gear pump, and the high-pressure degassed water flows to the reagent needle to clean the inner wall of the reagent needle. The reagent needle cleaning station is connected to the first centrifugal pump, and the system liquid flows into the reagent needle cleaning station to clean the outer wall of the reagent needle. The reagent needle cleaning solution storage station is connected to the cleaning solution bottle, and the cleaning solution in the cleaning solution bottle flows to the reagent needle cleaning solution storage station to clean the reagent on the inner wall of the reagent needle.

[0010] The cleaning and separation module includes a dispensing needle, a suction needle, a buffer bottle, a buffer control module, and a needle cleaning station. The buffer control module is connected to the degassing device. The low-pressure degassing water flows to the buffer control module. The buffer control module controls the buffer in the buffer bottle to flow to the dispensing needle and the suction needle. The needle cleaning station is connected to the first centrifugal pump. The system liquid flows into the needle cleaning station to clean the outer walls of the dispensing needle and the suction needle.

[0011] The detection module includes a measuring cell, an aspiration needle, a co-reaction liquid plunger pump, a co-reaction liquid bottle, and a co-reaction liquid buffer station. The measuring cell is connected to the degassing device, and the low-pressure degassing water flows to the measuring cell. The aspiration needle is connected to the measuring cell. The co-reaction liquid plunger pump and the cleaning liquid plunger pump are respectively connected to the degassing device. The co-reaction liquid plunger pump controls the co-reaction liquid bottle to supply liquid to the co-reaction liquid buffer station.

[0012] Furthermore, the liquid supply module also includes a five-way valve, a first throttle valve, and a pressure gauge. The five-way valve is connected to the first centrifugal pump, the degassing device, the liquid supply tank, and the pressure gauge, respectively. The liquid supply tank, the first centrifugal pump, and the five-way valve form a circuit. The first throttle valve is installed on the circuit. Adjusting the opening and closing degree of the first throttle valve adjusts the circuit pressure according to the pressure gauge reading.

[0013] Furthermore, the liquid supply module also includes a cooling water tank and a first three-way connector, which is connected to the five-way valve, the cooling water tank, and the degassing device, respectively.

[0014] Furthermore, the liquid supply module also includes a second three-way connector, a first six-way valve block, a four-way valve, and a second six-way valve block. The second three-way connector is connected to the output end of the degassing device, the input end of the gear pump, and the first six-way valve block, respectively. The low-pressure degassing water generated by the degassing device flows into the first six-way valve block through the second three-way connector, and the first six-way valve block outputs the low-pressure degassing water. The low-pressure degassing water generated by the degassing device flows into the gear pump through the second three-way connector for pressurization, and the resulting high-pressure degassing water flows to the second six-way valve block through the four-way valve, and the second six-way valve block outputs the high-pressure degassing water.

[0015] Furthermore, the sample dispensing module also includes a sample dispensing plunger pump and a clot detection structure. The sample dispensing plunger pump is connected to the second six-way valve block. The sample dispensing plunger pump controls the flow of the high-pressure degassed water to the reagent needle. The clot detection structure is located between the sample dispensing plunger pump and the reagent needle. The clot detection structure detects the high-pressure degassed water.

[0016] Furthermore, the sample loading module also includes a third six-way valve block, a third solenoid valve, and a pressure regulating valve. The third six-way valve block is connected to the first three-way connector, and the third solenoid valve and the pressure regulating valve are installed between the third six-way valve block and the sample loading needle cleaning station.

[0017] Furthermore, the reagent dispensing module also includes a fifth solenoid valve, a reagent dispensing plunger pump, and a sixth solenoid valve. The reagent dispensing plunger pump is connected to the second six-way valve block. The fifth solenoid valve is located between the second six-way valve block and the reagent dispensing plunger pump. The reagent dispensing plunger pump controls the cleaning of the reagent needle. The reagent needle cleaning station is connected to the third six-way valve block. The sixth solenoid valve is located between the reagent needle cleaning station and the third six-way valve block.

[0018] Furthermore, the cleaning and separation module also includes an eighth solenoid valve and a pre-cleaning plunger pump. The pre-cleaning plunger pump is connected to the first six-way valve block. The eighth solenoid valve is disposed between the first six-way valve block and the pre-cleaning plunger pump. The pre-cleaning plunger pump is connected to the buffer control module to control the buffer solution in the buffer bottle.

[0019] Furthermore, the detection module also includes a cleaning fluid plunger pump and a needle cleaning station, and the sample addition module also includes a cleaning fluid control module. The cleaning fluid plunger pump is connected to the cleaning fluid control module, and the cleaning fluid control module is connected to the cleaning fluid bottle. The cleaning fluid plunger pump controls the cleaning fluid to flow from the cleaning fluid bottle into the needle cleaning station through the cleaning fluid control module.

[0020] Furthermore, the liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer also includes a cooling module, which includes a reagent compartment and a second centrifugal pump. The reagent compartment and the second centrifugal pump are connected to the cooling water tank. The cooling water tank stores the system liquid and cools the system liquid. The second centrifugal pump draws the cooled system liquid from the cooling water tank to the reagent compartment for cooling.

[0021] Compared to existing technologies, in the fully automated electrochemiluminescence immunoassay analyzer of this invention, the liquid supply module's liquid supply tank is connected to a first centrifugal pump. The first centrifugal pump draws liquid from the liquid supply tank, and a portion of the drawn liquid forms a system liquid for cleaning. A degassing device is connected to the first centrifugal pump, and a portion of the liquid drawn by the first centrifugal pump is degassed by the degassing device to form low-pressure degassed water. A gear pump is connected to the degassing device, and the gear pump pressurizes a portion of the low-pressure degassed water to form high-pressure degassed water. The sample loading module's loading needle is connected to the gear pump, and high-pressure degassed water flows to the loading needle to clean the inner wall of the loading needle. The loading needle cleaning station is connected to the first centrifugal pump, and system liquid flows into the loading needle cleaning station to clean the outer wall of the loading needle. The cleaning solution bottle is connected to the loading needle cleaning solution storage station, and the cleaning solution in the cleaning solution bottle flows to the loading needle cleaning solution storage station to clean the reagents on the inner wall of the loading needle. The reagent loading module's reagent needle is connected to the gear pump, and high-pressure degassed water flows to the reagent needle to clean the inner wall of the reagent needle. The reagent needle cleaning station is connected to the first centrifugal pump, and the system liquid flows into the reagent needle cleaning station to clean the outer wall of the reagent needle. The reagent needle cleaning solution storage station is connected to the cleaning solution bottle, and the cleaning solution in the cleaning solution bottle flows to the reagent needle cleaning solution storage station to clean the reagent on the inner wall of the reagent needle. The buffer control module of the cleaning and separation module is connected to the degassing device, and the low-pressure degassing water flows to the buffer control module. The buffer control module controls the flow of the buffer solution in the buffer bottle to the dispensing needle and the aspiration needle. The needle group cleaning station is connected to the first centrifugal pump, and the system liquid flows into the needle group cleaning station to clean the outer wall of the dispensing needle and the aspiration needle. The measuring cell of the detection module is connected to the degassing device, and the low-pressure degassing water flows to the measuring cell. The aspiration needle is connected to the measuring cell. The co-reaction liquid plunger pump and the cleaning solution plunger pump are respectively connected to the degassing device. The co-reaction liquid plunger pump controls the co-reaction liquid bottle to supply liquid to the co-reaction liquid buffer station. Through the above design, the liquid circuit system of the fully automatic electrochemiluminescence immunoassay analyzer has high accuracy, high reliability, and high repeatability. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer of the present invention;

[0023] Figure 2 for Figure 1 A schematic diagram of the liquid supply module of the liquid circuit system of a fully automated electrochemiluminescence immunoassay analyzer;

[0024] Figure 3 for Figure 1 A schematic diagram of the sample loading module of the liquid circuit system of a fully automated electrochemiluminescence immunoassay analyzer;

[0025] Figure 4 for Figure 1 A schematic diagram of the reagent loading module of the liquid circuit system of a fully automated electrochemiluminescence immunoassay analyzer;

[0026] Figure 5 for Figure 1 A schematic diagram of the cleaning and separation module of the liquid circuit system of a fully automated electrochemiluminescence immunoassay analyzer;

[0027] Figure 6 for Figure 1 A schematic diagram of the cooling module of the liquid circuit system of a fully automated electrochemiluminescence immunoassay analyzer;

[0028] Figure 7 for Figure 1 A schematic diagram of the detection module of the liquid circuit system of a fully automated electrochemiluminescence immunoassay analyzer.

[0029] In the diagram: 10. Liquid supply module; 11. Filter; 12. First solenoid valve; 13. Liquid supply tank; 14. First centrifugal pump; 15. Five-way valve; 16. First throttle valve; 17. First three-way connector; 18. Cooling water tank; 19. Second throttle valve; 191. Degassing device; 192. Second three-way connector; 193. First six-way valve block; 194. Gear pump; 195. Four-way valve; 196. Second six-way valve block; 20. Sample dispensing module; 21. Second solenoid valve. 21. Solenoid valve; 22. Sample dispensing plunger pump; 23. Clot detection structure; 24. Dispensing needle; 250. Third six-way valve block; 251. Third solenoid valve; 252. Pressure regulating valve; 253. Dispensing needle cleaning station; 26. Cleaning solution bottle; 27. Cleaning solution control module; 28. Fourth solenoid valve; 29. ​​Dispensing needle cleaning solution storage station; 30. Reagent dispensing module; 31. Fifth solenoid valve; 32. Reagent dispensing plunger pump; 33. Reagent needle; 34. Sixth solenoid valve; 35. 36. Reagent needle cleaning station; 37. Seventh solenoid valve; 40. Reagent needle cleaning solution storage station; 41. Cleaning separation module; 42. Eighth solenoid valve; 43. Pre-cleaning plunger pump; 44. Buffer solution control module; 45. Ninth solenoid valve; 46. Tenth solenoid valve; 47. Eleventh solenoid valve; 48. Fifteenth solenoid valve; 49. Buffer solution bottle; 40. Dispensing needle; 40. Aspiration needle; 41. Twelfth solenoid valve; 42. First preheating device; 43. Needle assembly cleaning station 50. Refrigeration module; 51. Reagent compartment; 52. Second centrifugal pump; 60. Detection module; 61. Thirteenth solenoid valve; 62. Suction needle plunger pump; 63. Pipe clamp; 64. Measuring cell; 65. Suction needle; 66. Fourteenth solenoid valve; 67. Co-reaction liquid plunger pump; 68. Cleaning liquid plunger pump; 69. Co-reaction liquid bottle; 691. Co-reaction liquid control module; 692. Second preheating device; 693. Co-reaction liquid buffer station; 694. Suction needle cleaning station. Detailed Implementation

[0030] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or it can be fixed through another intermediate component. When a component is said to be "connected to" another component, it can be directly connected to the other component or it may be fixed through another intermediate component. When a component is said to be "set on" another component, it can be set directly on the other component or it may be set through another intermediate component. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0033] Please see Figure 1 The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer includes a liquid supply module 10, a sample loading module 20, a reagent loading module 30, a cleaning and separation module 40, a cooling module 50, and a detection module 60.

[0034] Please continue reading. Figure 2 The liquid supply module 10 includes a filter 11, a first solenoid valve 12, a liquid supply tank 13, a first centrifugal pump 14, a five-way valve 15, a first throttle valve 16, a first three-way connector 17, a cooling water tank 18, a second throttle valve 19, a degassing device 191, a second three-way connector 192, a first six-way valve block 193, a gear pump 194, a four-way valve 195, and a second six-way valve block 196.

[0035] Filter 11 is connected to the first solenoid valve 12, which in turn is connected to the supply water tank 13. Deionized water passes through filter 11 and the first solenoid valve 12 and enters the supply water tank 13 for buffering. The supply water tank 13 is connected to a five-way valve 15 via a first centrifugal pump 14. One end of the five-way valve 15 is connected to the supply water tank 13 via a first throttle valve 16 to form a loop. A pressure gauge is installed on the five-way valve 15. Specifically, the first centrifugal pump 14 is a magnetic centrifugal pump. Under the action of the magnetic centrifugal pump, deionized water returns to the supply water tank 13 through the five-way valve 15 to form a loop. The opening and closing degree of the first throttle valve 16 is adjusted so that the pressure gauge reading on the five-way valve 15 is 60 kPa.

[0036] One end of the first three-way connector 17 is connected to the five-way valve 15, the other end is connected to the cooling water tank 18, and the last end is connected to the second throttle valve 19 and the degassing device 191. The system liquid flowing out of the first three-way connector 17 is divided into two paths. One path is supplied to the cooling water tank 18 through the switch valve, and the other path is divided into two paths again through the three-way connector after passing through the switch valve. One path is supplied to each cleaning station through the second throttle valve 19 for cleaning the reagent needle, sample needle, and the outer wall of the cleaning separation needle. The other path is processed by the degassing device 191 to form low-pressure degassing water. The low-pressure degassing water is divided into two paths through the second three-way connector 192. One path is output as low-pressure degassing water through the first six-way valve block 193 to provide low-pressure degassing water for the pre-cleaning plunger pump 42 of the cleaning separation module 40 and the aspiration needle plunger pump, co-reaction liquid plunger pump 67, and cleaning liquid plunger pump 68 of the detection module 60. Another stream, after being precisely pressurized by gear pump 194, forms high-pressure degassed water. This high-pressure degassed water then passes through four-way valve 195 and the second six-way valve block 196 to supply the sample loading plunger pump 22 of sample loading module 20 and the reagent loading plunger pump 32 of reagent loading module 30 for high-pressure rinsing of the needle's inner wall, and to the aspiration needle cleaning station 694 of detection module 60 for high-pressure rinsing of the aspiration needle 65's outer wall. After being pressurized by gear pump 194, one branch of the four-way valve 195 returns to the water tank through a throttle valve and two three-way connectors to form a loop. By adjusting the opening and closing degree of the throttle valve and the pressure regulating knob of gear pump 194, the pressure gauge reading on the four-way valve 195 is made to be 0.3 MPa.

[0037] Please continue reading. Figure 3 The sample loading module 20 includes a second solenoid valve 21, a sample loading plunger pump 22, a clot detection structure 23, a loading needle 24, a third six-way valve block 250, a third solenoid valve 251, a pressure regulating valve 252, a loading needle cleaning station 253, a cleaning solution bottle 26, a cleaning solution control module 27, a fourth solenoid valve 28, and a loading needle cleaning solution storage station 29. The second solenoid valve 21 is connected to the second six-way valve block 196, and the sample loading plunger pump 22 is connected to the second solenoid valve 21 and the loading needle 24. Degassed high-pressure water is supplied to the sample loading plunger pump 22 through the second solenoid valve 21, and then to the loading needle 24 through the clot detection structure 23. Opening the second solenoid valve 21 allows for pure water cleaning of the needle's inner wall. The third six-way valve block 250 is connected to the first three-way connector 17. Undegassed low-pressure water (system fluid) passes through the third six-way valve block 250, the third solenoid valve 251, and the pressure regulating valve 252, and is supplied to the needle cleaning station 253. Opening the third solenoid valve 251 enables pure water cleaning of the needle's outer wall. Adjusting the pressure regulating valve 252 ensures a stable water flow and prevents splashing. The cleaning solution in the cleaning solution bottle 26 is supplied to the needle cleaning solution storage station 29 via the cleaning solution control module 27 and the fourth solenoid valve 28. The cleaning solution is cyclically supplied under the control of the cleaning solution control module 27. The needle 24 aspirates and discharges the cleaning solution to clean the reagents on the inner wall of the needle and prevents cross-contamination of samples.

[0038] Please continue reading. Figure 4 The reagent dispensing module 30 includes a fifth solenoid valve 31, a reagent dispensing plunger pump 32, a reagent needle 33, a sixth solenoid valve 34, a reagent needle cleaning station 35, a seventh solenoid valve 36, and a reagent needle cleaning solution storage station 37. The fifth solenoid valve 31 is connected to the second six-way valve block 196, and the reagent dispensing plunger pump 32 is connected to the reagent needle 33. High-pressure degassed water enters the two reagent dispensing plunger pumps 32 through the fifth solenoid valve 31, and the two reagent dispensing plunger pumps 32 supply the high-pressure degassed water to the two reagent needles 33. Opening the fifth solenoid valve 31 allows for pure water cleaning of the needle's inner wall. The sixth solenoid valve 34 is connected to the third six-way valve block 250, and undegassed low-pressure water is supplied to the reagent needle cleaning station 35 through the sixth solenoid valve 34 and a pressure regulating valve. Opening the sixth solenoid valve 34 allows for pure water cleaning of the needle's outer wall. The cleaning solution bottle 26 is connected to the seventh solenoid valve 36 through the cleaning solution control module 27, and the seventh solenoid valve 36 is connected to the reagent needle cleaning solution storage station 37. The cleaning solution in the cleaning solution bottle 26 is supplied to the reagent needle cleaning solution storage station 37 through the seventh solenoid valve 36. The cleaning solution is controlled by the cleaning solution control module 27 to achieve periodic circulation supply. The reagent needle 33 aspirates and discharges the cleaning solution to clean the reagent on the inner wall of the needle.

[0039] Please continue reading. Figure 5 The cleaning and separation module 40 includes an eighth solenoid valve 41, a pre-cleaning plunger pump 42, a buffer control module 43, a buffer bottle 44, a dispensing needle 45, a suction needle 46, a twelfth solenoid valve 47, a first preheating device 48, and a needle cleaning station 49. The eighth solenoid valve 41 is connected to a first six-way valve block 193. The pre-cleaning plunger pump 42 is connected to the buffer control module 43, which includes a ninth solenoid valve 430, a tenth solenoid valve 431, and an eleventh solenoid valve 432. Degassed low-pressure water is supplied to the pre-cleaning plunger pump 42 via the eighth solenoid valve 41, and then to the buffer control module 43. The ninth and tenth solenoid valves correspond to the inlet branch of the buffer bottle 44, and the eleventh solenoid valve 432 corresponds to the outlet branch of the dispensing needle 45. Both branches are connected to the pure water branch of the pre-cleaning plunger pump 42 via a buffer pipeline. The buffer temperature is maintained at 28±3℃ after being processed by the first preheating device 48. The fifteenth solenoid valve 433, connected to the buffer control module 43, corresponds to the aspiration needle 46 branch for aspirating and discharging impurities from the reaction solution. The solenoid valve at the bottom of the buffer control module 43 corresponds to the waste discharge branch for renewing the buffer solution in the pipeline. Undegassed low-pressure water is supplied to the needle assembly cleaning station 49 via the twelfth solenoid valve 47, the pressure regulating valve, and the first preheating device 48. Opening the twelfth solenoid valve 47 enables pure water cleaning of the needle's outer wall. Adjusting the pressure regulating valve ensures a smooth water flow and prevents splashing.

[0040] Please continue reading. Figure 6The refrigeration module 50 includes a reagent compartment 51 and a second centrifugal pump 52. The reagent compartment 51 and the second centrifugal pump 52 are connected to a cooling water tank 18. The cooling water tank 18 stores and cools the system liquid. The second centrifugal pump 52 draws the cooled system liquid from the cooling water tank 18 to the reagent compartment 51 for further cooling, and finally returns it to the cooling water tank 18, achieving a continuous cooling effect. Circulating low-temperature water keeps the refrigeration temperature in the reagent compartment 51 constant at 3–9°C, preventing the reaction reagents from deteriorating due to excessively high temperatures and affecting the test results. Simultaneously, it works in conjunction with the second preheating device 692 of the detection module 60 to control the temperature of the co-reaction solution and cleaning solution supplied by the detection module 60.

[0041] Please continue reading. Figure 7 The detection module 60 includes a thirteenth solenoid valve 61, a suction needle plunger pump 62, a valve clamp 63, a measuring cell 64, a suction needle 65, a fourteenth solenoid valve 66, a co-reaction liquid plunger pump 67, a cleaning liquid plunger pump 68, a co-reaction liquid bottle 69, a co-reaction liquid control module 691, a second preheating device 692, a co-reaction liquid buffer station 693, and a suction needle cleaning station 694. The thirteenth solenoid valve 61 is connected to the first six-way valve block 193. The suction needle plunger pump 62 is connected to the measuring cell 64 and the suction needle 65 via the valve clamp 63. Degassed low-pressure water is supplied to the suction needle plunger pump 62 through the thirteenth solenoid valve 61, and then splits into two paths: one path supplies the measuring cell 64 and the suction needle 65 via the valve clamp 63; the other path leads to the waste discharge point via the valve clamp 63. Degassed low-pressure water is supplied to the co-reaction liquid plunger pump 67 and the cleaning liquid plunger pump 68 via the fourteenth solenoid valve 66, and then to their respective flow path modules. Taking the co-reaction liquid control module 691 as an example, two branches are connected to the pure water branch of the co-reaction liquid plunger pump 67 via connecting and buffer pipelines. After being treated by the second preheating device 692, the temperature of the co-reaction liquid is maintained at 28±3℃. The bottom solenoid valve corresponds to the waste discharge branch for refreshing the co-reaction liquid in the pipeline. The connection relationship and function of the cleaning liquid control module 27 and the co-reaction liquid control module 691 are basically the same. The difference is that the cleaning liquid not only flows to the co-reaction liquid buffer station 693 via a solenoid valve, but also flows to the sample needle cleaning liquid storage station 29 and the reagent needle cleaning liquid storage station 37 via solenoid valves respectively. Degassed high-pressure water is supplied to the aspiration needle cleaning station 694 via a solenoid valve and the second preheating device 629. Opening the solenoid valve can realize pure water high-pressure cleaning of the needle outer wall.

[0042] In the liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer of this invention, the liquid supply module 10's liquid supply tank 13 is connected to the first centrifugal pump 14. The first centrifugal pump 14 draws liquid from the liquid supply tank 13, and a portion of the liquid drawn by the first centrifugal pump 14 forms a system liquid for cleaning. The degassing device 191 is connected to the first centrifugal pump 14, and a portion of the liquid drawn by the first centrifugal pump 14 is degassed by the degassing device 191 to form low-pressure degassed water. The gear pump 194 is connected to the degassing device 191, and the gear pump 194 pressurizes a portion of the low-pressure degassed water to form high-pressure degassed water. The sample loading module 20... The sample needle 24 is connected to the gear pump 194. High-pressure degassed water flows to the sample needle 24 to clean its inner wall. The sample needle 24 cleaning station is connected to the first centrifugal pump 14. System liquid flows into the sample needle 24 cleaning station to clean its outer wall. The cleaning solution bottle 26 is connected to the sample needle 24 cleaning solution storage station. The cleaning solution in the cleaning solution bottle 26 flows to the sample needle 24 cleaning solution storage station to clean the reagent on the inner wall of the sample needle 24. The reagent needle 33 of the reagent dispensing module 30 is connected to the gear pump 194. High-pressure degassed water flows to the reagent needle 33 to clean its inner wall. The cleaning station 33 is connected to the first centrifugal pump 14. System liquid flows into the cleaning station 33 to clean the outer wall of the reagent needle 33. The cleaning solution storage station 33 is connected to the cleaning solution bottle 26. The cleaning solution in the cleaning solution bottle 26 flows to the cleaning solution storage station 33 to clean the reagent on the inner wall of the reagent needle 33. The buffer solution control module 43 of the cleaning and separation module 40 is connected to the degassing device 191. Low-pressure degassing water flows to the buffer solution control module 43. The buffer solution control module 43 controls the flow of buffer solution in the buffer solution bottle 44 to the dispensing needle 45 and the aspiration needle 46. The needle cleaning station 49 is connected to the first centrifugal pump 14. The heart pump 14 is connected, and the system liquid flows into the needle cleaning station 49 to clean the outer walls of the distribution needle 45 and the aspiration needle 46; the measuring cell 64 of the detection module 60 is connected to the degassing device 191, and low-pressure degassing water flows to the measuring cell 64. The aspiration needle 65 is connected to the measuring cell 64. The co-reaction liquid plunger pump 67 and the cleaning liquid plunger pump 68 are respectively connected to the degassing device 191. The co-reaction liquid plunger pump 67 controls the co-reaction liquid bottle 69 to supply liquid to the co-reaction liquid buffer station 693. Through the above design, the liquid circuit system of the fully automatic electrochemiluminescence immunoassay analyzer has high accuracy, good reliability and high repeatability.

[0043] The above embodiments merely illustrate several implementation methods of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present invention. These are all equivalent modifications and improvements made to the above embodiments based on the essential technology of the present invention, and all of these fall within the protection scope of the present invention.

Claims

1. The liquid circuit system of a fully automated electrochemiluminescence immunoassay analyzer, comprising a liquid supply module, a sample loading module, a reagent loading module, a cleaning and separation module, and a detection module, characterized in that: The liquid supply module includes a liquid supply tank, a first centrifugal pump, a degassing device, and a gear pump. The liquid supply tank is connected to the first centrifugal pump, which draws liquid from the liquid supply tank. A portion of the liquid drawn by the first centrifugal pump forms a system liquid for cleaning. The degassing device is connected to the first centrifugal pump, which degasses a portion of the liquid drawn by the first centrifugal pump to form low-pressure degassed water. The gear pump is connected to the degassing device, which pressurizes a portion of the low-pressure degassed water to form high-pressure degassed water. The sample loading module includes a loading needle, a loading needle cleaning station, a cleaning solution bottle, and a loading needle cleaning solution storage station. The loading needle is connected to the gear pump, and the high-pressure degassed water flows to the loading needle to clean the inner wall of the loading needle. The loading needle cleaning station is connected to the first centrifugal pump, and the system liquid flows into the loading needle cleaning station to clean the outer wall of the loading needle. The cleaning solution bottle is connected to the loading needle cleaning solution storage station, and the cleaning solution in the cleaning solution bottle flows to the loading needle cleaning solution storage station to clean the reagents on the inner wall of the loading needle. The reagent dispensing module includes a reagent needle, a reagent needle cleaning station, and a reagent needle cleaning solution storage station. The reagent needle is connected to the gear pump, and the high-pressure degassed water flows to the reagent needle to clean the inner wall of the reagent needle. The reagent needle cleaning station is connected to the first centrifugal pump, and the system liquid flows into the reagent needle cleaning station to clean the outer wall of the reagent needle. The reagent needle cleaning solution storage station is connected to the cleaning solution bottle, and the cleaning solution in the cleaning solution bottle flows to the reagent needle cleaning solution storage station to clean the reagent on the inner wall of the reagent needle. The cleaning and separation module includes a dispensing needle, a suction needle, a buffer bottle, a buffer control module, and a needle cleaning station. The buffer control module is connected to the degassing device. The low-pressure degassing water flows to the buffer control module. The buffer control module controls the buffer in the buffer bottle to flow to the dispensing needle and the suction needle. The needle cleaning station is connected to the first centrifugal pump. The system liquid flows into the needle cleaning station to clean the outer walls of the dispensing needle and the suction needle. The detection module includes a measuring pool, an aspiration needle, a co-reaction liquid plunger pump, a co-reaction liquid bottle, and a co-reaction liquid buffer station. The measuring pool is connected to the degassing device, and the low-pressure degassing water flows to the measuring pool. The aspiration needle is connected to the measuring pool. The co-reaction liquid plunger pump and the cleaning liquid plunger pump are respectively connected to the degassing device. The co-reaction liquid plunger pump controls the co-reaction liquid bottle to supply liquid to the co-reaction liquid buffer station. The liquid supply module also includes a five-way valve, a first throttle valve, and a pressure gauge. The five-way valve is respectively connected to the first centrifugal pump, the degassing device, the liquid supply tank, and the pressure gauge. The liquid supply tank, the first centrifugal pump, and the five-way valve form a loop. The first throttle valve is installed on the loop. The opening and closing degree of the first throttle valve is adjusted, and the loop pressure is adjusted according to the pressure gauge reading.

2. The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer according to claim 1, characterized in that: The liquid supply module also includes a cooling water tank and a first three-way connector, which is connected to the five-way valve, the cooling water tank and the degassing device respectively.

3. The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer according to claim 2, characterized in that: The liquid supply module further includes a second three-way connector, a first six-way valve block, a four-way valve, and a second six-way valve block. The second three-way connector is connected to the output end of the degassing device, the input end of the gear pump, and the first six-way valve block, respectively. The low-pressure degassing water generated by the degassing device flows into the first six-way valve block through the second three-way connector, and the first six-way valve block outputs the low-pressure degassing water. The low-pressure degassing water generated by the degassing device flows into the gear pump through the second three-way connector for pressurization, and the resulting high-pressure degassing water flows to the second six-way valve block through the four-way valve, and the second six-way valve block outputs the high-pressure degassing water.

4. The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer according to claim 3, characterized in that: The sample dispensing module also includes a sample dispensing plunger pump and a clot detection structure. The sample dispensing plunger pump is connected to the second six-way valve block. The sample dispensing plunger pump controls the flow of the high-pressure degassed water to the reagent needle. The clot detection structure is located between the sample dispensing plunger pump and the reagent needle. The clot detection structure detects the high-pressure degassed water.

5. The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer according to claim 3, characterized in that: The sample loading module also includes a third six-way valve block, a third solenoid valve, and a pressure regulating valve. The third six-way valve block is connected to the first three-way connector, and the third solenoid valve and the pressure regulating valve are installed between the third six-way valve block and the sample loading needle cleaning station.

6. The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer according to claim 5, characterized in that: The reagent dispensing module further includes a fifth solenoid valve, a reagent dispensing plunger pump, and a sixth solenoid valve. The reagent dispensing plunger pump is connected to the second six-way valve block. The fifth solenoid valve is located between the second six-way valve block and the reagent dispensing plunger pump. The reagent dispensing plunger pump controls the cleaning of the reagent needle. The reagent needle cleaning station is connected to the third six-way valve block. The sixth solenoid valve is located between the reagent needle cleaning station and the third six-way valve block.

7. The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer according to claim 3, characterized in that: The cleaning and separation module further includes an eighth solenoid valve and a pre-cleaning plunger pump. The pre-cleaning plunger pump is connected to the first six-way valve block. The eighth solenoid valve is located between the first six-way valve block and the pre-cleaning plunger pump. The pre-cleaning plunger pump is connected to the buffer control module to control the buffer solution in the buffer bottle.

8. The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer according to claim 3, characterized in that: The detection module further includes a cleaning fluid plunger pump and a needle cleaning station. The sample addition module further includes a cleaning fluid control module. The cleaning fluid plunger pump is connected to the cleaning fluid control module, and the cleaning fluid control module is connected to the cleaning fluid bottle. The cleaning fluid plunger pump controls the cleaning fluid to flow from the cleaning fluid bottle into the needle cleaning station through the cleaning fluid control module.

9. The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer according to claim 2, characterized in that: The liquid circuit system of the fully automated electrochemiluminescence immunoassay analyzer also includes a cooling module, which includes a reagent compartment and a second centrifugal pump. The reagent compartment and the second centrifugal pump are connected to the cooling water tank. The cooling water tank stores the system liquid and cools the system liquid. The second centrifugal pump draws the cooled system liquid from the cooling water tank to the reagent compartment for cooling.

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