Induction device for rapid propagation of tissue culture woody flowers

The induction device for wood plants stabilizes temperature, humidity, and light conditions while preventing bottle movement, reducing damage and enhancing retrieval efficiency.

CN120304300APending Publication Date: 2025-07-15FUJIAN YANGTA HORTICULTURE CO LTD
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Patent Information

Application Number
CN202510669580.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-23
Publication Date
2025-07-15

AI Technical Summary

Technical Problem

During the tissue culture process, the culture bottle is prone to accidentally touching, moving or falling, resulting in tissue culture property loss and waste of culture tissue.

Method used

A tissue culture woody flower rapid breeding induction device is designed, including the bottom assembly, the middle assembly and the top assembly. The culture bottle is fixed by the anti-fall assembly, combined with the air guide cylinder and the LED spotlight to provide appropriate temperature and light, avoid the drop of the culture bottle, and adjust the humidity through water mist and airflow.

Benefits of technology

Effectively prevent culture bottles from falling, reduce operational risks, reduce tissue culture costs, improve induction efficiency, and ensure that culture bottles reproduce rapidly under optimal temperature, humidity and light conditions.

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Abstract

The invention provides an induction device for rapid propagation of tissue culture woody flowers, and relates to the technical field of tissue culture induction. The device comprises a bottom assembly, a middle assembly, an anti-falling assembly and a top assembly, the bottom assembly is used for generating vertically-upward water mist with the required temperature, the middle assembly is arranged above the bottom assembly and comprises a middle shell, the lower portion of the middle shell is fixedly connected with an insertion column, and the insertion column is fixedly connected with the top assembly. Observation pieces are symmetrically and rotationally connected to the middle shell through hinges, clamping parts are arranged between the observation pieces and the middle shell, an air guide barrel is fixedly connected to the center of the middle shell, and a middle insertion barrel is fixedly connected to the inner bottom wall of the middle shell. By arranging the anti-falling assembly, the culture bottle can be fixed, the culture bottle is prevented from falling and being damaged due to accidental touch, the operation risk is reduced, and the tissue culture cost is reduced.
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Description

Technical Field

[0001] The present invention relates to the technical field of tissue culture induction, and specifically to an induction device for rapid propagation of tissue-cultured woody flowers. Background Art

[0002] Plant tissue culture (hereinafter referred to as tissue culture) is a technique for obtaining complete plants by culturing a part of a plant (such as shoot tips, leaves, roots, etc.) under aseptic conditions in an artificially controlled environment. During this tissue culture process, the division ability of explant cells is activated through hormone regulation or mechanical damage; structures such as clustered buds, adventitious buds, or embryoids are induced to differentiate from the explants, providing a basis for subsequent proliferation.

[0003] In tissue culture, according to the plant culture stage, the composition of the culture medium used is different. For example, during primary culture, high-concentration cytokinins are selected to induce bud differentiation, combined with low-concentration auxins; during subculture proliferation, the hormone ratio is adjusted to maintain the continuous proliferation of clustered buds; during rooting induction, low-concentration auxins or hormones are removed.

[0004] In related technologies, such as a rapid propagation culture medium and culture method for woody flower plants with the publication number of CN114208670B, the composition of the induction medium, proliferation medium, and rooting medium is disclosed.

[0005] During tissue culture induction, an induction device is required. For example, an induction device for rapid propagation of tissue-cultured flowers with the publication number of CN222603364U discloses the lighting lamps required for induction, providing the light intensity during the tissue culture process.

[0006] When using the induction device, it is usually necessary to place the culture bottle containing the culture medium and the culture tissue in the induction device. However, multiple culture bottles are usually directly arranged and placed in one area. When taking and placing one culture bottle, it is easy to accidentally touch the nearby culture bottles, and the culture bottles will move or even fall, resulting in property losses in tissue culture and waste of the culture tissue. Summary of the Invention

[0007] Aiming at the deficiencies of the prior art, the present invention provides an induction device for rapid propagation of tissue-cultured woody flowers, which solves the problem that the culture bottles are easily accidentally touched, moved, or even dropped.

[0008] To achieve the above objectives, the present invention is realized through the following technical solutions: An induction device for rapid propagation of tissue-cultured woody flowers, comprising:

[0009] A bottom component for generating water mist with the required temperature vertically upward;

[0010] A middle component, the middle component is arranged above the bottom component, the middle component includes an intermediate shell, a plug column is fixedly connected to the lower part of the intermediate shell, an observation sheet is symmetrically rotatably connected to the intermediate shell through a hinge, a clamping component is arranged between the observation sheet and the intermediate shell, an air guide tube is fixedly connected to the center of the intermediate shell, a middle plug tube is fixedly connected to the inner bottom wall of the intermediate shell, anti-drop components are evenly arranged on the circumference of the air guide tube, LED spotlights are evenly arranged on the circumference directly below the anti-drop components, a guide groove is arranged at the gap between the LED spotlights, and a water leakage hole is arranged at the end of the guide groove;

[0011] The anti-drop assembly comprises an outer shell and an inner shell, the outer shell and the inner shell are mutually adsorbed via an adsorption surface, an airflow cavity is provided in the outer shell and the inner shell, an air outlet is provided on the inner side of the outer shell and the inner shell, a notch is provided on the side wall where the air outlet is located, an oblique expansion is provided at the notch on the bottom side, a space for placing a glass culture bottle is reserved between the outer shell and the inner shell, and the LED spotlight can illuminate the bottom surface of the glass culture bottle;

[0012] The top component is arranged above the middle component and is used to cover and seal the middle component. The anti-drop component can fix the culture bottle to avoid the culture bottle from being accidentally touched and falling and being damaged, reduce the operation risk, and reduce the tissue culture cost; the middle component and the bottom component can provide suitable temperature and humidity for the flower tissue, induce the flower component to reproduce quickly, improve the induction efficiency, and can take multiple culture bottles at one time, and take them quickly.

[0013] Preferably, the bottom component includes a bottom shell fixedly mounted on a support frame, a heater is mounted in the bottom shell, a liquid inlet pipe, a liquid discharge pipe and a ventilation pipe are provided on the bottom shell, a bottom insert is fixedly connected to the inner circumference of the bottom shell, a water surface frame is fixedly connected in the bottom shell and above the heater, a water inlet frame is fixedly connected in the middle of the water surface frame, a liquid absorbent cotton column is provided in the water inlet frame, a ceramic atomizing sheet is provided at the upper end of the liquid absorbent cotton column, a top sheet is fixedly connected in the bottom shell and above the water surface frame, a mounting frame is fixedly connected in the middle of the top sheet, an exhaust fan is fixedly mounted on the mounting frame, and a make way jack and a liquid return port are provided on the mounting frame.

[0014] Preferably, an outer retaining ring is fixedly connected to the upper surface of the outer shell, an inner retaining ring is fixedly connected to the upper surface of the inner shell, and the inner shell and the air guide tube are fixedly connected and communicated with each other.

[0015] Preferably, the top component includes a top cover, and a handle is fixedly connected to the top cover.

[0016] Preferably, it also includes a support frame, which is fixedly installed at the bottom of the bottom component and is used to provide supporting force for the bottom component.

[0017] Preferably, the snap-fitting component comprises a buckle and a socket, the buckle is fixedly mounted on the observation piece, the socket is fixedly mounted on the intermediate shell, and the observation piece is made of a transparent material.

[0018] Preferably, the mounting frame is formed by welding a plurality of legs evenly distributed around the circumference, the ceramic atomizing sheet is located directly below the legs, and the cross-sectional shape of the legs is an inverted triangle.

[0019] Preferably, the heating tube of the heater is placed in the bottom shell, the positions of the clearance hole and the bottom insert tube correspond to each other, the plug column is adapted to pass through the clearance hole and the bottom insert tube, and the middle insert tube is adapted to the plug column.

[0020] The present invention provides an induction device for rapid propagation of tissue culture woody flowers. It has the following beneficial effects:

[0021] 1. The present invention can fix the culture bottle by setting the anti-drop component, avoid the culture bottle from being accidentally touched and falling and being damaged, reduce the operation risk, and reduce the tissue culture cost; and cooperate with the air guide tube to provide the best temperature and humidity for the glass culture bottle, thereby improving the induction success rate.

[0022] 2. The present invention can provide suitable temperature and humidity for flower tissues by setting up the middle component and the bottom component, induce the rapid reproduction of the flower components, improve the induction efficiency, and can take multiple culture bottles at one time, and take them quickly. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is an overall stereogram of the present invention;

[0024] Figure 2 is a perspective view of the middle component of the present invention;

[0025] Figure 3 It is a disassembled stereogram of the middle component of the present invention;

[0026] Figure 4 The figure is a disassembled stereoscopic view of the middle component of the present invention from a bottom perspective;

[0027] Figure 5 It is a three-dimensional diagram of the water surface frame and the water entry frame of the present invention;

[0028] Figure 6 is a perspective view of the middle component of the present invention;

[0029] Figure 7 A top view of the middle component of the present invention;

[0030] Figure 8 A perspective view of the middle component of the present invention with the observation piece opened;

[0031] Figure 9 is a three-dimensional diagram of the anti-drop assembly of the present invention;

[0032] Figure 10 A bottom perspective view of the anti-drop assembly of the present invention;

[0033] Figure 11 for Figure 10 The enlarged view of point A in the middle;

[0034] Figure 12 A three-dimensional diagram of the anti-drop assembly of the present invention in a disassembled state;

[0035] Figure 13 It is a three-dimensional view of the top component of the present invention.

[0036] Among them, 1. Support frame; 2. Bottom component; 3. Middle component; 4. Top component; 5. Anti-drop component; 201. Bottom shell; 202. Top sheet; 203. Make way jack; 204. Liquid return port; 205. Mounting frame; 206. Ventilation pipe; 207. Liquid inlet pipe; 208. Exhaust fan; 209. Heater; 210. Water surface frame; 211. Water inlet frame; 212. Liquid absorbing cotton column; 213. Ceramic atomizing sheet; 214. Bottom insert; 215. Liquid discharge pipe; 301 , middle shell; 302, observation piece; 303, plug column; 304, air guide tube; 305, middle plug tube; 306, snap-on component; 307, hinge; 308, water leakage hole; 309, guide groove; 310, LED spotlight; 501, outer shell; 502, inner shell; 503, outer retaining ring; 504, inner retaining ring; 505, air outlet; 506, notch; 507, airflow cavity; 508, oblique expansion; 509, adsorption surface; 401, handle; 402, top cover. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] like Figures 1-13 As shown, an embodiment of the present invention provides an induction device for rapid propagation of tissue culture woody flowers, comprising:

[0039] A bottom component 2, used for generating water mist of a desired temperature vertically upward;

[0040] The bottom component 2 includes a bottom shell 201 fixedly mounted on the support frame 1, a heater 209 is mounted in the bottom shell 201, a liquid inlet pipe 207, a liquid discharge pipe 215, and a ventilation pipe 206 are arranged on the bottom shell 201, a bottom insert 214 is fixedly connected to the inner circumference of the bottom shell 201, a water surface frame 210 is fixedly connected in the bottom shell 201 and located above the heater 209, a water inlet frame 211 is fixedly connected in the middle of the water surface frame 210, a liquid absorbing cotton column 212 is arranged in the water inlet frame 211, a ceramic atomizing sheet 213 is arranged at the upper end of the liquid absorbing cotton column 212, a top sheet 202 is fixedly connected in the bottom shell 201 and located above the water surface frame 210, a mounting frame 205 is fixedly connected in the middle of the top sheet 202, an exhaust fan 208 is fixedly mounted on the mounting frame 205, and a clearance jack 203 and a liquid return port 204 are arranged on the mounting frame 205;

[0041] refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , the liquid inlet pipe 207 is arranged at the upper part of the bottom shell 201 for adding water; the liquid discharge pipe 215 is arranged at the bottom of the bottom shell 201 for draining water; there are at least two ventilation pipes 206 for the outside airflow to enter the bottom shell 201 and the airflow in the bottom shell 201 to enter the outside; the heater 209 has a thermostat for controlling the heating temperature for obtaining water at a required temperature; the water inlet rack 211 is located below the water surface, the liquid absorbing cotton column 212 can absorb water, and use the capillary phenomenon to supply water to the ceramic atomizing sheet 213, and the ceramic atomizing sheet 213 can have multiple pieces to increase the atomization amount and meet the humidity requirements. The water mist atomized by the ceramic atomizing sheet 213 can be discharged by the exhaust fan 208 Blow upward from bottom to top, so that the water mist of the target temperature can enter the middle component 3; the return liquid port 204 is used for the excess water to return to the bottom shell 201; the water volume in the bottom shell 201 can be detected by a water level sensor, and the water temperature can be detected by a temperature sensor; the return liquid port 204 is arc-shaped and there are multiple ones, which can be suitable for the return liquid flow at various positions; a filter net can be added to the ventilation pipe 206 to prevent external impurities from entering therein, thereby improving the stability and cleanliness of the internal environment of the bottom shell 201; because the exhaust fan 208 is higher than the top plate 202, the exhaust fan 208 will extend into the air guide tube 304, so that the water mist can be completely transported and avoid the dispersion of the water mist.

[0042] The heating tube of the heater 209 is placed in the bottom shell 201, the positions of the clearance hole 203 and the bottom plug-in tube 214 correspond to each other, the plug-in column 303 passes through the clearance hole 203 and fits with the bottom plug-in tube 214, and the middle plug-in tube 305 fits with the plug-in column 303;

[0043] refer to Figure 4 , Figure 3, the insertion post 303 can be inserted into the bottom insertion cylinder 214 through the relief insertion hole 203, facilitating the installation of the middle component 3 into the bottom component 2. The insertion post 303 can also be inserted into the middle insertion cylinder 305 for the mutual installation of multiple middle components 3. A permanent magnet can be fixedly installed at the bottom of the insertion post 303, and another permanent magnet is fixedly installed in the bottom insertion cylinder 214 and the middle insertion cylinder 305. The two permanent magnets attract each other, and it can be determined whether the insertion post 303 is inserted in place according to whether the two permanent magnets are attracted.

[0044] The mounting frame 205 is formed by welding multiple circumferentially uniformly distributed feet. The ceramic atomizing sheet 213 is located directly below the feet, and the cross-sectional shape of the feet is an inverted triangle.

[0045] Reference Figure 2 , Figure 3 , since the water mist generated by the ceramic atomizing sheet 213 is in a horn shape, when the water mist moves upward, it will encounter the feet with an inverted triangle cross-section. Under the action of the feet, the water mist will be separated into two parts, and the two parts will respectively enter the gaps between adjacent feet, so as to ensure that the water mist amount between adjacent feet is the same, ensure that the water mist can be evenly blown up by the exhaust fan 208, ensure that the water mist delivery amount in each place is the same, and is conducive to ensuring good humidity uniformity.

[0046] The middle component 3 is arranged above the bottom component 2. The middle component 3 includes an intermediate shell 301. An insertion post 303 is fixedly connected to the lower part of the intermediate shell 301. Observation pieces 302 are symmetrically rotatably connected to the intermediate shell 301 through hinges 307. A clamping component 306 is arranged between the observation pieces 302 and the intermediate shell 301. A wind guide cylinder 304 is fixedly connected to the center of the intermediate shell 301. A middle insertion cylinder 305 is fixedly connected to the inner bottom wall of the intermediate shell 301. Anti-falling components 5 are circumferentially uniformly arranged on the wind guide cylinder 304. LED spotlights 310 are circumferentially uniformly arranged directly below the anti-falling components 5. Guide grooves 309 are arranged at the gaps of the LED spotlights 310, and a water leakage hole 308 is arranged at the end of the guide grooves 309.

[0047] Reference Figure 6 , Figure 7 , Figure 8, the middle shell 301 is provided with a taking ear, which is convenient for taking the middle component 3 as a whole; the air guide tube 304 is used for the circulation of the atomized air flow, so that the target temperature air flow can enter the anti-drop component 5 to provide the required temperature and humidity for the glass culture bottle. The light emitted by the LED spotlight 310 can irradiate the bottom of the glass culture bottle. Due to the refraction and diffuse reflection of light by the glass culture bottle, light will be generated on the entire body of the glass culture bottle. This light surrounds the cultured tissue for one week. Therefore, the uniformity of the light source can be guaranteed, and the required light for the culture combination can be guaranteed to be uniform; the LED spotlight 310 arranged at the bottom can prevent other glass culture bottles from affecting the light irradiation, ensuring that each glass culture bottle can obtain the best light irradiation; the guiding groove 309 is used to guide the flow direction of the excess water flow, so that the water flow can enter the water leakage hole 308. When there are multiple middle components 3, the water flow falling from the water leakage hole 308 of the upper middle component 3 will fall into the lower middle component 3. Since the positions of the water leakage holes 308 are the same, the water flow will not stay in the lower middle component 3 and will only directly fall into the bottom shell 201 at the bottom, thus being able to avoid excessive humidity at the bottom middle component 3 and ensuring that the humidity of each middle component 3 is within a suitable range; the humidity inside the middle shell 301 can be detected by a humidity sensor.

[0048] The clamping component 306 includes a buckle and a clamping seat. The buckle is fixedly installed on the observation piece 302, and the clamping seat is fixedly installed on the middle shell 301. The observation piece 302 is made of a transparent material;

[0049] Reference Figure 8 , the observation piece 302 can be opened and closed, which is convenient for taking out the glass culture bottle. The transparent observation piece 302 can also directly observe the internal situation. Transparent materials such as glass and acrylic plates can be selected for the transparent material. The cross-sectional shape of the observation piece 302 is arc-shaped; in order to ensure the sealing performance at the observation piece 302, a sealing strip can be added at the edge of the observation piece 302.

[0050] The anti-drop component 5 includes an outer shell 501 and an inner shell 502. The outer shell 501 and the inner shell 502 are adsorbed to each other through an adsorption surface 509. An air flow cavity 507 is provided in both the outer shell 501 and the inner shell 502. An air outlet 505 is provided on the inner side surfaces of the outer shell 501 and the inner shell 502. A notch 506 is provided on the side wall where the air outlet 505 is located, and an inclined expansion port 508 is provided at the notch 506 at the bottom side. A space for placing the glass culture bottle is reserved between the outer shell 501 and the inner shell 502. The LED spotlight 310 can irradiate the bottom surface of the glass culture bottle;

[0051] Reference Figure 9 , Figure 10 , Figure 11 , Figure 12, the adsorption surfaces 509 of the outer housing 501 and the inner housing 502 can be made of permanent magnets that attract each other, so that the outer housing 501 can be tightly adsorbed to the inner housing 502. The outer housing 501 and the inner housing 502 can be used to limit the glass culture bottle, prevent the glass culture bottle from falling, reduce the loss of tissue culture, and at the same time, it is also convenient to take, with simple and reliable operation;

[0052] When the anti-falling component 5 works specifically: the target temperature atomized water mist in the air guide cylinder 304 enters the air flow cavity 507 in the inner housing 502, then enters the air flow cavity 507 in the outer housing 501, and then blows out from the air outlet 505 onto the outer surface of the glass culture bottle. Since the relative humidity of the air is usually 80%, the water mist with higher humidity will adhere to the outer surface of the glass culture bottle, and the small water droplets will converge into large water droplets. Then, under the action of gravity, they will flow downward at the notch 506 and then fall into the guiding groove 309, so as to facilitate falling from the water leakage hole 308, and the water droplets will reflect and refract light, thereby enhancing the uniformity of the light source and ensuring the uniformity of the light source for the cultured tissue;

[0053] The outer housing 501 and the inner housing 502 are located at a position slightly above the middle of the glass culture bottle, so that the water droplets formed by the water mist can flow through the entire outer surface of the glass culture bottle to the greatest extent, and the water droplets will reflect light. The light passing through the entire outer wall of the glass culture bottle can reflect the light everywhere, so that the light can shine on the cultured tissue to the greatest extent, enabling different parts of the tissue culture to receive more and more uniform light. In order to ensure that the light is maximally irradiated into the glass culture bottle, the outer housing 501 and the inner housing 502 can be made of light-proof or light-reflecting materials to ensure that the cultured tissue can grow upward smoothly;

[0054] When it is necessary to take the glass culture bottle, the glass culture bottle can be directly taken from above, or the observation piece 302 can be opened, the outer housing 501 can be removed, and the glass culture bottle can be taken out, or the entire middle component 3 can be directly removed at once. Multiple middle components 3 can be classified and placed according to the type of cultured tissue, which is convenient for tissue culture use and more suitable for actual use;

[0055] Among them, the inclined flared opening 508 is used for the air flow at the bottom notch 506 to be blown out obliquely. The inclined air flow blows the water flow in the guiding groove 309 into the water leakage hole 308, accelerating the discharge and falling of the water flow. At the same time, the gas at the target temperature can surround the glass culture bottle, so that the glass culture bottle is always in an environment with the best temperature and humidity, avoiding the difference between the temperature and humidity at the glass culture bottle and the best temperature and humidity, and ensuring the smooth progress of induction.

[0056] The upper surface of the outer housing 501 is fixedly connected with an outer retaining ring 503, the upper surface of the inner housing 502 is fixedly connected with an inner retaining ring 504, and the inner housing 502 is fixedly connected with and communicates with the air guide cylinder 304;

[0057] refer to Figure 9 The inner retaining ring 504 and the outer retaining ring 503 are used to block the airflow so that the airflow can only blow out downwards and will not blow out upwards from the notch 506, thereby enhancing the airflow strength and allowing the airflow to surround the glass culture bottle to provide optimal temperature and humidity, while also facilitating the water flow in the guide groove 309 to blow into the water leakage hole 308.

[0058] The top component 4 is disposed above the middle component 3 and is used to cover and seal the middle component 3; the top component 4 includes a top cover 402, and a handle 401 is fixedly connected to the top cover 402;

[0059] refer to Figure 13 The top cover 402 can be removed through the handle 401. After the top cover 402 is covered, the upper part of the middle component 3 can be covered to prevent the atomized water mist from floating out and ensure the stability of the humidity inside the middle component 3.

[0060] It also includes a support frame 1, which is fixedly installed at the bottom of the bottom component 2 and is used to provide support force for the bottom component 2;

[0061] refer to Figure 1 The support frame 1 can be welded with square tubes to provide support for the whole. A controller can be provided on the support frame 1 to control the temperature, humidity and operation of the exhaust fan 208 and the ceramic atomizing sheet 213.

[0062] Working principle: When in use, insert the plug 303 of the middle component 3 through the yielding hole 203 into the bottom plug tube 214, put in the first middle component 3, then put the prepared glass culture bottle in the anti-drop component 5, then use the plug 303 to insert into the middle plug tube 305, put in the second middle component 3, put the glass culture bottle in the anti-drop component 5 of the second middle component 3, select the appropriate number of middle components 3 according to the number to be placed, and then cover the top cover 402 on the uppermost middle component 3;

[0063] Then, a proper amount of water is added from the liquid inlet pipe 207, and the water is heated to a target temperature, such as 25°C, by using the heater 209, and then the ceramic atomizing sheet 213 and the exhaust fan 208 are started, the ceramic atomizing sheet 213 generates water mist, and the exhaust fan 208 blows the water mist upward;

[0064] The target temperature atomized water mist in the air duct 304 enters the air flow cavity 507 in the inner housing 502, then enters the air flow cavity 507 in the outer housing 501, and then blows onto the outer surface of the glass culture bottle from the air outlet 505. Since the relative humidity of the air is usually 80%, the water mist with higher humidity will adhere to the outer surface of the glass culture bottle, and small water droplets will converge into large water droplets. Then, under the action of gravity, the water droplets will flow downward at the notch 506 and then fall into the guiding groove 309, so as to facilitate falling from the water leakage hole 308. And the water droplets will reflect and refract light, thereby enhancing the uniformity of the light source and ensuring the uniformity of the light source for the cultured tissue. The inclined flared opening 508 is used for the air flow at the bottom notch 506 to be blown out obliquely, and the inclined air flow blows the water flow in the guiding groove 309 into the water leakage hole 308 to accelerate the discharge and fall of the water flow;

[0065] The adsorption surface 509 tightly adsorbs the outer housing 501 and the inner housing 502 together. The outer housing 501 and the inner housing 502 can limit the glass culture bottle to prevent the glass culture bottle from falling and reduce the loss of tissue culture;

[0066] When it is necessary to take the glass culture bottle, the glass culture bottle can be directly taken from above, or the observation piece 302 can be opened, the outer housing 501 can be removed, and the glass culture bottle can be taken out, or the entire middle component 3 can be directly removed at once. Multiple middle components 3 can be classified and placed according to the type of cultured tissue, which is convenient for tissue culture use and more suitable for the actual use situation.

[0067] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirits of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An induction device for rapid propagation of tissue-cultured woody flowers, characterized in that, include: A bottom component (2) is used to generate water mist of a desired temperature vertically upward; The middle component (3) is arranged above the bottom component (2), and the middle component (3) comprises an intermediate shell (301). The lower part of the intermediate shell (301) is fixedly connected with a plug post (303). The intermediate shell (301) is symmetrically rotatably connected with an observation piece (302) via a hinge (307). A clamping component (306) is provided between the observation piece (302) and the intermediate shell (301). 1) is fixedly connected to the center of the intermediate shell (301), a middle insert cylinder (305) is fixedly connected to the inner bottom wall of the intermediate shell (301), anti-drop components (5) are evenly arranged on the circumference of the air guide cylinder (304), LED spotlights (310) are evenly arranged on the circumference directly below the anti-drop components (5), a guide groove (309) is arranged at the gap between the LED spotlights (310), and a water leakage hole (308) is arranged at the end of the guide groove (309); The anti-drop assembly (5) comprises an outer shell (501) and an inner shell (502); the outer shell (501) and the inner shell (502) are mutually adsorbed via an adsorption surface (509); an airflow cavity (507) is provided in the outer shell (501) and the inner shell (502); an air outlet (505) is provided on the inner side surface of the outer shell (501) and the inner shell (502); a notch (506) is provided on the side wall where the air outlet (505) is located; an oblique expansion (508) is provided at the notch (506) on the bottom side; a space for placing a glass culture bottle is reserved between the outer shell (501) and the inner shell (502); and the LED spotlight (310) can illuminate the bottom surface of the glass culture bottle; A top component (4), wherein the top component (4) is arranged above the middle component (3) and is used to cover and seal the middle component (3).

2. The induction device for rapid propagation of tissue-cultured woody flowers according to claim 1, characterized in that: The bottom assembly (2) comprises a bottom shell (201) fixedly mounted on the support frame (1), a heater (209) being mounted in the bottom shell (201), a liquid inlet pipe (207), a liquid discharge pipe (215), and a ventilation pipe (206) being provided on the bottom shell (201), a bottom insert (214) being fixedly connected to the inner circumference of the bottom shell (201), a water surface frame (210) being fixedly connected in the bottom shell (201) and located above the heater (209), and an inlet pipe (214) being fixedly connected to the middle of the water surface frame (210). A water frame (211), wherein a liquid absorbing cotton column (212) is arranged in the water inlet frame (211), and a ceramic atomizing sheet (213) is arranged at the upper end of the liquid absorbing cotton column (212). A top sheet (202) is fixedly connected in the bottom shell (201) and located above the water surface frame (210), and a mounting frame (205) is fixedly connected in the middle of the top sheet (202), and an exhaust fan (208) is fixedly installed on the mounting frame (205). The mounting frame (205) is provided with a clearance jack (203) and a liquid return port (204).

3. The induction device for rapid propagation of tissue-cultured woody flowers according to claim 1, characterized in that: An outer retaining ring (503) is fixedly connected to the upper surface of the outer shell (501), an inner retaining ring (504) is fixedly connected to the upper surface of the inner shell (502), and the inner shell (502) and the air guide cylinder (304) are fixedly connected and communicate with each other.

4. The induction device for rapid propagation of tissue-cultured woody flowers according to claim 1, characterized in that: The top assembly (4) comprises a top cover (402), and a handle (401) is fixedly connected to the top cover (402).

5. The induction device for rapid propagation of tissue-cultured woody flowers according to claim 1, wherein: It also comprises a support frame (1), wherein the support frame (1) is fixedly mounted on the bottom of the bottom component (2) and is used to provide supporting force for the bottom component (2).

6. The induction device for rapid propagation of tissue-cultured woody flowers according to claim 1, characterized in that: The snap-fit component (306) comprises a snap buckle and a snap seat, the snap buckle is fixedly mounted on the observation piece (302), the snap seat is fixedly mounted on the intermediate shell (301), and the observation piece (302) is made of a transparent material.

7. An induction device for rapid propagation of tissue-cultured woody flowers according to claim 1, characterized in that: The mounting frame (205) is formed by welding a plurality of supporting legs evenly distributed around a circumference, the ceramic atomizing sheet (213) is located directly below the supporting legs, and the cross-sectional shape of the supporting legs is an inverted triangle.

8. An induction device for rapid propagation of tissue-cultured woody flowers according to claim 2, characterized in that: The heating tube of the heater (209) is placed in the bottom shell (201), the positions of the clearance hole (203) and the bottom plug tube (214) correspond to each other, the plug column (303) passes through the clearance hole (203) and is adapted to the bottom plug tube (214), and the middle plug tube (305) is adapted to the plug column (303).

Citation Information

Patent Citations

  • A rapid propagation culture medium and cultivation method for woody flowering plants

    CN114208670B

  • Tissue culture flower rapid propagation induction device

    CN222603364U