Storage device and method for maintaining biomechanical strength of bone tissue

Abstract

The present invention belongs to the technical field of bone tissue storage, and specifically relates to a storage device and method for maintaining the biomechanical strength of bone tissue, comprising: a storage barrel for storing bone tissue; a storage tank, a sealing cover being detachably mounted on the top of the storage tank, a fixed tube for injecting liquid nitrogen being fixed on the outer wall of the storage tank; a support plate, the support plate being fixed inside the storage tank, the support plate dividing the inner cavity of the storage tank from top to bottom into a storage cavity and a liquid nitrogen cavity by the support plate, a through groove one being provided on the support plate, cold air generated by evaporation of liquid nitrogen being introduced into the storage cavity through the through groove one to store the storage barrel at a low temperature; the present invention recovers the cold air into the liquid nitrogen cavity before removing the storage barrel by arranging a gas recovery component, effectively avoiding leakage of cold air during the process of removing the storage barrel, thereby saving resources and preventing injury from cold air leakage; bone tissue can be stored by using the device and method to maintain the biomechanical strength of the bone tissue to the maximum extent.

Description

Technical Field

[0001] The present invention belongs to the technical field of bone tissue storage, and in particular to a storage device and method for maintaining the biomechanical strength of bone tissue. Background Art

[0002] With the continuous development of the biomedical field, especially in tissue engineering, transplant medicine, and cell therapy, the demand for biological specimen preservation technologies continues to increase. The long-term preservation of bone tissue, bone marrow, and other tissues often requires cryopreservation methods. Liquid nitrogen preservation technology, due to its superior low-temperature stability, is widely used for the long-term storage of biological tissues and cells. Liquid nitrogen storage tanks, due to their ability to provide a low-temperature environment of -196°C, have become a common tool for preserving biological specimens such as bone tissue.

[0003] During the operation of liquid nitrogen storage tanks, accessing stored samples often requires opening the tank lid or other sealing device, resulting in evaporation of liquid nitrogen and leakage of large amounts of cold air. Since liquid nitrogen quickly vaporizes into nitrogen gas at room temperature, this process not only leads to instability in the cryogenic environment, but also may result in waste of liquid nitrogen resources. More seriously, if the operator does not operate properly, he may suffer frostbite from exposure to the low-temperature liquid nitrogen cold air.

[0004] To this end, the present invention provides a storage device and method for maintaining the biomechanical strength of bone tissue. Summary of the Invention

[0005] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0006] The technical solution adopted by the present invention to solve the technical problem is: the storage device for maintaining the biomechanical strength of bone tissue of the present invention comprises:

[0007] A storage barrel, wherein the storage barrel is used to store bone tissue;

[0008] A storage tank, wherein a sealing cover is detachably mounted on the top of the storage tank, and a fixed tube for injecting liquid nitrogen is fixed on the outer wall of the storage tank;

[0009] A support plate is fixed to the interior of the storage tank, and the support plate divides the inner cavity of the storage tank from top to bottom into a placement cavity and a liquid nitrogen cavity. The storage barrel is placed in the placement cavity, and the liquid nitrogen cavity stores liquid nitrogen. A through groove 1 is formed on the support plate, and cold air generated by evaporation of liquid nitrogen is introduced into the placement cavity through the through groove 1 to store the storage barrel at a low temperature.

[0010] A gas recovery assembly is used to recover cold gas into the liquid nitrogen chamber before the preservation barrel is taken out. The gas recovery assembly includes a sealing ring that is sealingly and slidingly installed inside the storage tank. The sealing ring is sealingly and slidingly sleeved on the outside of the preservation barrel. A sleeve rod is fixed on the support plate. A movable rod is slidingly installed in the middle of the sleeve rod. The top of the movable rod is fixedly connected to the sealing ring. A screw is threadedly connected to the middle of the movable rod. A motor that drives the screw to rotate is fixed on the top of the storage tank.

[0011] Preferably, a lifting assembly is further included, by which the preservation barrel is lifted, the lifting assembly includes a cylinder fixed in the middle of the liquid nitrogen chamber, a piston plate is sealingly and slidably installed inside the cylinder, a connecting rod is fixed on the top of the piston plate, a groove is provided in the center of the upper end surface of the support plate, a top plate is provided in the groove, the connecting rod slides through the through holes provided at corresponding positions on the cylinder and the support plate and is fixed to the bottom of the top plate, a connecting pipe is fixed to the lower side wall of the cylinder, and the connecting pipe is connected to the placement chamber away from one end of the cylinder.

[0012] Preferably, a blocking component is provided on the support plate, by which the through slot 1 is placed in an open or closed state. The blocking component includes a movable block, a sliding cavity is horizontally opened in the middle of the through slot 1, and the movable block is slidably arranged in the sliding cavity. A spring 1 is fixed inside the sliding cavity to reset the movable block, and a magnetic block is fixed to the bottom end of the movable rod, and the magnetic block attracts the movable block to slide along the sliding cavity through magnetic force.

[0013] Preferably, a second through slot is provided in the middle of the movable block, a retaining ring is fixed in the middle of the second through slot, a flap is fitted above the retaining ring, and the flap is rotatably mounted on the inner wall of the second through slot via a torsion spring.

[0014] Preferably, a pressure relief assembly is provided in the lifting assembly, and the pressure relief assembly includes an air outlet opened at the top of the cylinder, an installation cavity is opened inside the piston plate, and through holes are provided on the upper and lower sides of the installation cavity. A movable plate is slidably installed in the middle of the installation cavity, and a push rod is fixed on the top of the movable plate. The push rod extends to the top of the piston plate through the through hole, and a second spring is provided under the movable plate, and the movable plate is pushed up by the second spring to block the through hole above.

[0015] Preferably, a limit ring is fixed to the upper inner wall of the storage tank.

[0016] Preferably, a vertical rod is slidably installed in the vertical groove opened in the middle of the connecting rod, and a push block is slidably installed in the horizontal groove opened in the middle of the piston plate. The push block is formed by a combination of two right-angled trapezoidal blocks, and a spring three is installed inside the horizontal groove to drive the push block to move horizontally. The top end of the vertical rod extends to above the top plate, and the bottom end of the vertical rod extends to the inside of the horizontal groove and abuts against the inclined surface of the left trapezoidal block in the push block. The movable plate is provided with a frustum shape, and the inclined surface of the trapezoidal block on the right side of the push block is adapted to the inclined surface of the movable plate.

[0017] Preferably, a threaded hole is provided in the middle of the sealing cover, a threaded rod is threadedly connected in the threaded hole, a pressure plate is rotatably mounted on the bottom end of the threaded rod, and a turning handle is fixed on the top end of the threaded rod.

[0018] Preferably, a sealing ring is fixed on the lower end surface of the limiting ring and located at the edge of the center hole.

[0019] A method for storing and maintaining the biomechanical strength of bone tissue, using the above-mentioned storage device for maintaining the biomechanical strength of bone tissue, comprises the following steps:

[0020] S1. Place the bone tissue into a preservation bucket filled with biological medium;

[0021] S2: The motor drives the screw to rotate, pushing the movable rod down along the sleeve rod, thereby driving the sealing ring down to squeeze the low-temperature gas below, and introducing the low-temperature gas in the placement chamber into the cylinder through the connecting pipe, emptying the low-temperature gas in the placement chamber, and pushing the piston plate, connecting rod and movable plate up;

[0022] S3. In step S2, during the downward movement of the movable rod, the driving magnetic block and the movable block are dislocated, and the movable block is pushed by the spring to move and close the through slot, so that the cold air enters the cylinder through the connecting pipe;

[0023] S4. The sealing ring continues to move downward until it fits into the support plate. During this period, the excess cold air inside the cylinder is discharged into the liquid nitrogen chamber through the pressure relief component.

[0024] S5. Open the sealing cover and insert the storage barrel along the center hole of the limiting ring until it contacts the top plate;

[0025] S6. Control the motor to reverse, driving the lead screw, movable rod, and sealing ring to move in the opposite direction, generating negative pressure inside the placement chamber, drawing the cryogenic gas at the bottom of the piston plate into the placement chamber, and simultaneously driving the flap to rotate upward and open, allowing the cryogenic gas in the liquid nitrogen chamber to enter the placement chamber through the second through slot in the middle of the movable block;

[0026] S7. After the sealing plate moves up to the initial position, the movable plate moves down to the groove above the support plate, the storage barrel is completely placed in the placement cavity, the sealing cover is installed, and finally the threaded rod is rotated to drive the pressing plate to press on the top of the storage barrel to complete the storage work.

[0027] The beneficial effects of the present invention are as follows:

[0028] 1. The storage device and method for maintaining the biomechanical strength of bone tissue described in the present invention recovers cold air into the liquid nitrogen chamber before the storage barrel is removed by providing a gas recovery component. When the storage barrel needs to be removed, the motor drives the screw to rotate, thereby driving the movable rod to move downward along the sleeve rod, and synchronously driving the sealing ring to move downward until it is completely in contact with the support plate. The sealing ring squeezes the cold air in the cavity below and recovers all the cold air inside the placement chamber into the liquid nitrogen chamber. The sealing ring and the support plate are tightly fitted together to prevent the cold air from flowing from the liquid nitrogen chamber to the placement chamber. In this way, when opening the sealing cover to remove the storage barrel, cold air leakage is effectively avoided, thereby saving resources and preventing cold air leakage from injuring people. By storing bone tissue using this device and method, the biomechanical strength of the bone tissue can be maintained to the greatest extent.

[0029] 2. The storage device and method for maintaining the biomechanical strength of bone tissue described in the present invention elevates the preservation barrel by providing a lifting assembly. When the sealing ring moves downward, it simultaneously squeezes the cold air in the cavity below. The cold air is introduced into the interior of the cylinder and gathers under the piston plate, pushing the piston plate, connecting rod and top plate upward, and simultaneously lifting the preservation barrel upward, so that the top end of the preservation barrel protrudes from the opening at the top of the storage tank, making it easy to take out the preservation barrel and improving the taking efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The present invention will be further described below with reference to the accompanying drawings.

[0031] Figure 1 It is a perspective view of the present invention;

[0032] Figure 2 This is the internal structure of the storage barrel of the present invention;

[0033] Figure 3 It is a partial structural cross-sectional view of the present invention;

[0034] Figure 4 It is a structural schematic diagram of the lifting assembly in the present invention;

[0035] Figure 5 yes Figure 4 Enlarged view of point A in the middle;

[0036] Figure 6 is a cross-sectional view of the plugging assembly of the present invention;

[0037] Figure 7 yes Figure 6 Enlarged view of point B in the middle;

[0038] Figure 8 yes Figure 7 Enlarged view of point C in the middle;

[0039] Figure 9 It is a flow chart of the method of the present invention.

[0040] In the figure: 1. Storage tank; 2. Sealing cover; 3. Fixed tube; 4. Support plate; 5. Limiting ring; 6. Sealing ring; 7. Preservation barrel; 8. Placement chamber; 9. Liquid nitrogen chamber; 10. Screw; 11. Movable rod; 12. Through groove 1; 13. Sleeve rod; 14. Sealing ring; 15. Turning handle; 16. Threaded rod; 17. Pressing plate; 18. Cylinder; 19. Connecting rod; 20. Connecting tube; 21. Piston plate; 22. Groove; 23. Top plate; 24. Movable plate; 25. Push rod; 26. Spring 1; 27. Air vent; 28. Magnetic block; 29. Movable block; 30. Sliding chamber; 31. Spring 2; 32. Retaining ring; 33. Flip plate; 34. Through groove 2; 35. Vertical rod; 36. Spring 3; 37. Push block. DETAILED DESCRIPTION

[0041] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0042] Example 1: Figures 1 to 8 As shown, the storage device for maintaining the biomechanical strength of bone tissue according to an embodiment of the present invention includes:

[0043] A storage barrel 7, wherein the storage barrel 7 is used to store bone tissue;

[0044] A storage tank 1, wherein a sealing cover 2 is detachably mounted on the top of the storage tank 1, and a fixed pipe 3 for injecting liquid nitrogen is fixed on the outer wall of the storage tank 1;

[0045] A support plate 4 is fixed to the interior of the storage tank 1. The support plate 4 divides the inner cavity of the storage tank 1 from top to bottom into a placement cavity 8 and a liquid nitrogen cavity 9. The storage barrel 7 is placed in the placement cavity 8. The liquid nitrogen cavity 9 stores liquid nitrogen. A through groove 12 is formed on the support plate 4. The cold air generated by the evaporation of liquid nitrogen is introduced into the placement cavity 8 through the through groove 12 to store the storage barrel 7 at a low temperature.

[0046] A gas recovery assembly, through which the cold gas is recovered into the liquid nitrogen chamber 9 before the storage barrel 7 is taken out, includes a sealing ring 6 that is sealingly and slidably mounted inside the storage tank 1, and the sealing ring 6 is sealingly and slidably sleeved on the outside of the storage barrel 7. A sleeve rod 13 is fixed to the support plate 4, and a movable rod 11 is slidably mounted in the middle of the sleeve rod 13. The top of the movable rod 11 is fixedly connected to the sealing ring 6, and a screw rod 10 is threadedly connected to the middle of the movable rod 11. A motor that drives the screw rod 10 to rotate is fixed to the top of the storage tank 1;

[0047] When the bottle 7 is in working order, the bottle 7 is placed in the middle of the upper end surface of the support plate 4, and the sealing ring 6 is sleeved on the outer side of the bottle 7. A cavity for temporarily storing cold air is formed between the sealing ring 6, the storage tank 1, the support plate 4 and the bottle 7. The cold air generated by the evaporation of the liquid nitrogen in the liquid nitrogen chamber 9 enters the cavity through the through groove, and the bottle 7 is preserved at low temperature to maintain the activity of the bone tissue; when the bottle 7 needs to be taken out, the gas recovery component is started, the motor drives the screw rod 10 to rotate, and then drives the movable rod 11 to move downward along the sleeve rod 13, and synchronously drives the sealing ring 6 to move downward until it is completely in contact with the support plate 4. The sealing ring 6 squeezes the cold air in the cavity below and recovers all the cold air inside the placement cavity 8 into the liquid nitrogen chamber 9, and the sealing ring 6 and the support plate 4 are in close contact, preventing the cold air from flowing from the liquid nitrogen chamber 9 to the placement cavity 8. In this way, when the sealing cover 2 is opened to take out the bottle 7, the leakage of cold air is effectively avoided, so as to save resources and avoid the leakage of cold air to hurt people.

[0048] It also includes a lifting assembly, by which the preservation barrel 7 is lifted, the lifting assembly includes a cylinder 18 fixed in the middle of the liquid nitrogen chamber 9, a piston plate 21 is sealed and slidably installed inside the cylinder 18, a connecting rod 19 is fixed to the top of the piston plate 21, a groove 22 is opened in the center of the upper end surface of the support plate 4, a top plate 23 is provided in the groove 22, the connecting rod 19 slides through the through holes opened at corresponding positions on the cylinder 18 and the support plate 4 and is fixed to the bottom of the top plate 23, a connecting pipe 20 is fixed to the lower side wall of the cylinder 18, and the connecting pipe 20 is connected to the placement chamber 8 at one end away from the cylinder 18;

[0049] During operation, when the sealing ring 6 moves downward, it synchronously squeezes the cold air in the placement chamber 8 below. The cold air is introduced into the cylinder 18 and gathers under the piston plate 21. The volume of the placement chamber 8 is larger than the volume of the cylinder 18, so there is enough gas to push the piston plate 21, the connecting rod 19 and the top plate 23 upward, and synchronously lift the preservation barrel 7 upward, so that the top of the preservation barrel 7 protrudes from the opening at the top of the storage tank 1, making it convenient to take out the preservation barrel 7 and improving the taking efficiency.

[0050] The support plate 4 is provided with a blocking component, by which the through slot 12 is in an open or closed state. The blocking component includes a movable block 29. A sliding cavity 30 is horizontally opened in the middle of the through slot 12. The movable block 29 is slidably arranged in the sliding cavity 30. A spring 26 is fixed inside the sliding cavity 30 to reset the movable block 29. A magnetic block 28 is fixed to the bottom end of the movable rod 11. The magnetic block 28 attracts the movable block 29 to slide along the sliding cavity 30 through magnetic force.

[0051] During operation, when the preservation barrel 7 is located inside the storage tank 1 for low-temperature preservation, the movable rod 11 is at the topmost position, and the magnetic block 28 at the bottom of the movable rod 11 is aligned with the movable block 29. The movable block 29 is attracted by magnetic force to move laterally along the sliding cavity 30, and the spring 1 26 is compressed, so that the through groove 12 is in a conducting state, so that the cold air in the liquid nitrogen cavity 9 enters the placement cavity 8 through the through groove and contacts the side wall of the preservation barrel 7 to perform low-temperature preservation on the preservation barrel 7; when the screw rod 10 drives the movable rod 11 down, the movable block 29 is attracted by magnetic force to move laterally along the sliding cavity 30, and the spring 1 26 is compressed, so that the through groove 12 is in a conducting state, so that the cold air in the liquid nitrogen cavity 9 enters the placement cavity 8 through the through groove and contacts the side wall of the preservation barrel 7 to perform low-temperature preservation on the preservation barrel 7; When the movable block 29 is shifted, the screw rod 10 drives the magnetic block 28 to move downward, so that the magnetic block 28 and the movable block 29 are misaligned. At this time, the magnetic block 28 no longer attracts the movable block 29, and the movable block 29 is pushed back to its original position under the rebound action of the spring 12, so that the movable block 29 closes the through groove 12. At this time, the cold air in the placement chamber 8 cannot enter the liquid nitrogen chamber 9 through the through groove 12, and the cold air can only be pressed into the cylinder 18 through the connecting pipe 20, so as to drive the lifting assembly to move, thereby lifting the preservation barrel 7 and facilitating the removal of the preservation barrel 7.

[0052] A second through slot 34 is provided in the middle of the movable block 29, a retaining ring 32 is fixed in the middle of the second through slot 34, a flap 33 is fitted above the retaining ring 32, and the flap 33 is rotatably mounted on the inner wall of the second through slot 34 via a torsion spring; during operation, when the placement control motor is reversed, the screw rod 10, the movable rod 11 and the sealing ring 6 are driven to move in opposite directions, negative pressure is generated inside the placement chamber 8, and the flap 33 is driven to rotate upward and open, so that the cold air in the liquid nitrogen chamber 9 enters the placement chamber 8 through the second through slot 34 in the middle of the movable block 29, and stores the preservation barrel 7 at a low temperature.

[0053] The lifting assembly is provided with a pressure relief assembly, which includes an air outlet 27 opened at the top of the cylinder 18, a mounting cavity opened inside the piston plate 21, through holes are provided on the upper and lower sides of the mounting cavity, and a movable plate 24 is slidably installed in the middle of the mounting cavity, a push rod 25 is fixed to the top of the movable plate 24, the diameter of the push rod 25 is smaller than the inner diameter of the through hole above the mounting cavity, the push rod 25 extends to the top of the piston plate 21 through the through hole, and a spring 231 is provided below the movable plate 24, which pushes the movable plate 24 upward by the spring 231 to block the through hole above.

[0054] During operation, the cold air in the placement chamber 8 is pressed into the bottom of the piston plate 21, pushing the piston plate 21 upward until the pad on the top of the piston plate 21 contacts the inner wall of the top of the cylinder 18. During this process, the push rod 25 first contacts the top of the cylinder 18 and is squeezed and retracted into the installation chamber. At this time, the movable plate 24 is separated from the top of the installation chamber, and the cold air under the piston plate 21 can enter the upper space through the installation chamber and be discharged into the liquid nitrogen chamber 9 through the air outlet 27. This can reduce the pressure inside the cylinder 18 and avoid excessive pressure inside the cylinder 18, which may cause damage to the cylinder 18.

[0055] As the cold air in the placement chamber 8 is gradually discharged into the liquid nitrogen chamber 9, the pressure inside the cylinder 18 tends to stabilize. At this time, the movable plate 24 will gradually move upward under the action of the spring 2 31 and close the top through-hole of the installation chamber, preventing the gas under the piston plate 21 from further escaping, so that the piston plate 21, the connecting rod 19 and the top plate 23 can stably support the storage barrel 7.

[0056] In addition, a vertical notch is opened on the side of the movable plate 24, and the notch is set at the edge of the movable plate 24. When the movable plate 24 is in contact with the inner wall of the top of the installation cavity, there will be no air leakage in the notch. When the movable plate 24 moves downward, the gas flow rate is increased through the notch, which facilitates the accelerated discharge of excess gas.

[0057] A limiting ring 5 is fixed to the inner wall of the upper part of the storage tank 1; when working, the size of the middle hole of the limiting ring 5 is adapted to the outer diameter of the storage barrel 7. When placing the storage barrel 7, the storage barrel 7 is lowered from the middle hole of the limiting ring 5, and the storage barrel 7 is limited in the horizontal direction by the limiting ring 5 to avoid horizontal movement during transportation; in addition, the outer wall of the storage barrel 7 fits with the middle hole of the limiting ring 5, and when the storage barrel 7 is lowered through the middle hole, there is friction between the storage barrel 7 and the inner wall of the middle hole of the limiting ring 5, which causes the storage barrel 7 to be lowered slowly to avoid damage to the storage barrel 7.

[0058] A vertical rod 35 is slidably installed in the vertical groove opened in the middle of the connecting rod 19, and a push block 37 is slidably installed in the horizontal groove opened in the middle of the piston plate 21. The push block 37 is formed by a combination of two right-angled trapezoidal blocks. A spring 36 for driving the push block 37 to move horizontally is installed inside the horizontal groove. The top end of the vertical rod 35 extends above the top plate 23, and the bottom end of the vertical rod 35 extends into the inside of the horizontal groove and abuts against the inclined surface of the left trapezoidal block in the push block 37. The movable plate 24 is provided with a frustum shape, and the inclined surface of the trapezoidal block on the right side of the push block 37 is adapted to the inclined surface of the movable plate 24;

[0059] During operation, the storage barrel 7 is raised by the lifting assembly, and then the storage barrel 7 is taken out. At this time, the restriction on the top of the vertical rod 35 is removed, and the push block 37 is driven to move horizontally by the rebound of the spring 36, pushing the vertical rod 35 upward through the inclined surface of the left trapezoidal block, and making the end of the trapezoidal block on the right side of the push block 37 squeeze the upper inclined surface of the movable plate 24, driving the movable plate 24 to move downward. At this time, the installation cavity is connected, and the piston plate 21, the connecting rod 19 and the pressure plate 17 are affected by gravity and automatically descend, thereby discharging the gas under the piston plate 21 until the pressure plate 17 moves down to the groove 22 on the top of the support plate 4; When a new storage barrel 7 is subsequently placed, the storage barrel 7 can move downward along the middle hole of the limiting ring 5 until the bottom contacts the top of the support plate 4. When the sealing ring 6 is driven to move upward, the sealing ring 6 squeezes the air above and discharges the air from the through hole opened on the limiting ring 5, reducing the pressure between the limiting ring 5 and the sealing ring 6. At the same time, the cold air in the liquid nitrogen chamber 9 is discharged upward into the cavity below the sealing ring 6 through the middle through groove 2 34 of the movable block 29. During the movement of the sealing ring 6, it always fits the outer wall of the storage barrel 7, effectively preventing the leakage of cold air. The cold air contacts the side wall of the storage barrel 7, preserving the storage barrel 7 at a low temperature.

[0060] In addition, the storage barrel 7 can press down the vertical rod 35, and the bottom end of the vertical rod 35 contacts the inclined surface of the trapezoidal block on the left side of the push block 37, thereby driving the push block 37 to separate from the movable plate 24, and the spring 2 31 drives the movable plate 24 to return to the through hole at the top of the closed installation cavity, making advance preparations for the next lifting work.

[0061] A threaded hole is provided in the middle of the sealing cover 2, and a threaded rod 16 is threadedly connected to the threaded hole. A pressure plate 17 is rotatably installed at the bottom end of the threaded rod 16, and a turning handle 15 is fixed to the top of the threaded rod 16. When working, the preservation barrel 7 is first lowered from the middle hole of the limiting ring 5, and then the sealing cover 2 is tightened into the open top of the storage tank 1. After that, the threaded rod 16 is rotated by the turning handle 15 to drive the pressure plate 17 to move downward and press on the top of the preservation barrel 7, so as to limit the preservation barrel 7 in the vertical direction, so that the preservation barrel 7 can be stably stored inside the storage tank 1.

[0062] Example 2: Figures 1 to 4 As shown, comparative example 1, another embodiment of the present invention is: a sealing ring 14 is fixed on the lower end surface of the limiting ring 5 and at the edge of the center hole, and the sealing ring 14 is a hollow structure and is made of elastic material; when working, when the sealing ring 6 moves toward the limiting ring 5, the sealing ring 14 is squeezed by the sealing ring 6 and the limiting ring 5, and the sealing ring 14 is deformed and fits precisely with the outer wall of the preservation barrel 7, thereby further preventing cold air leakage.

[0063] like Figure 9 As shown, a method for storing the biomechanical strength of bone tissue is provided, which uses the above-mentioned storage device for storing the biomechanical strength of bone tissue and includes the following steps:

[0064] S1. Place the bone tissue into a storage barrel 7 filled with a biological medium;

[0065] S2. The motor drives the screw 10 to rotate, pushing the movable rod 11 downward along the sleeve rod 13, thereby driving the sealing ring 6 downward to squeeze the low-temperature gas below. The low-temperature gas in the placement chamber 8 is introduced into the cylinder 18 through the connecting pipe 20, and the low-temperature gas in the placement chamber 8 is evacuated. The piston plate 21, connecting rod 19 and movable plate 24 are pushed upward.

[0066] S3. In step S2, during the downward movement of the movable rod 11, the driving magnetic block 28 and the movable block 29 are dislocated, and the movable block 29 is pushed by the spring to move and close the through slot, so that the cold air enters the cylinder 18 through the connecting pipe 20;

[0067] S4, the sealing ring 6 continues to move downward until it fits into the support plate 4, during which the excess cold air inside the cylinder 18 is discharged into the liquid nitrogen chamber 9 through the pressure relief assembly;

[0068] S5. Open the sealing cover 2 and insert the storage barrel 7 along the center hole of the limiting ring 5 until it contacts the top plate 23.

[0069] S6. Control the motor to reverse, driving the screw 10, movable rod 11, and sealing ring 6 to move in the opposite direction, generating negative pressure inside the placement chamber 8, drawing the low-temperature gas at the bottom of the piston plate 21 into the placement chamber 8, and simultaneously driving the flap 33 to rotate upward and open, allowing the low-temperature gas in the liquid nitrogen chamber 9 to enter the placement chamber 8 through the second through groove 34 in the middle of the movable block 29;

[0070] S7. After the sealing plate moves up to the initial position, the movable plate 24 moves down to the groove 22 above the support plate 4, the storage barrel 7 is completely received in the placement cavity 8, the sealing cover 2 is installed, and finally the threaded rod 16 is rotated to drive the pressing plate 17 to press on the top of the storage barrel 7 to complete the storage work.

[0071] The above-mentioned front, back, left, right, up and down are all based on the Figure 1 As a benchmark, according to the person's observation perspective, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0072] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the scope of protection of the present invention.

[0073] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the foregoing embodiments. The foregoing embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A storage device for maintaining the biomechanical strength of bone tissue, characterized by: include: A storage barrel (7), wherein the storage barrel (7) is used to store bone tissue; A storage tank (1), wherein a sealing cover (2) is detachably mounted on the top of the storage tank (1), and a fixed tube (3) for injecting liquid nitrogen is fixed on the outer wall of the storage tank (1); A support plate (4), wherein the support plate (4) is fixed inside the storage tank (1), and the inner cavity of the storage tank (1) is divided from top to bottom into a placement cavity (8) and a liquid nitrogen cavity (9) by the support plate (4), wherein the storage barrel (7) is placed in the placement cavity (8), and liquid nitrogen is stored in the liquid nitrogen cavity (9), and a through groove (12) is provided on the support plate (4), and cold air generated by evaporation of liquid nitrogen is introduced into the placement cavity (8) through the through groove (12) to store the storage barrel (7) at a low temperature; A gas recovery assembly, through which cold gas is recovered into the liquid nitrogen chamber (9) before the storage barrel (7) is taken out, the gas recovery assembly comprises a sealing ring (6) sealingly slidably mounted inside the storage tank (1), the sealing ring (6) sealingly slidably sleeved on the outside of the storage barrel (7), a sleeve rod (13) fixed on the support plate (4), a movable rod (11) slidably mounted in the middle of the sleeve rod (13), the top end of the movable rod (11) fixedly connected to the sealing ring (6), the middle of the movable rod (11) threadedly connected to a screw rod (10), and a motor for driving the screw rod (10) to rotate fixed on the top of the storage tank (1); The storage barrel (7) is also included in the lifting assembly, and the lifting assembly is used to lift the storage barrel (7), and the lifting assembly includes a cylinder (18) fixed in the middle of the liquid nitrogen chamber (9), a piston plate (21) is sealed and slidably installed inside the cylinder (18), a connecting rod (19) is fixed on the top of the piston plate (21), a groove (22) is provided at the center of the upper end surface of the support plate (4), a top plate (23) is provided in the groove (22), the connecting rod (19) slides through the through holes provided at corresponding positions on the cylinder (18) and the support plate (4) and is fixed to the bottom of the top plate (23), a connecting pipe (20) is fixed to the lower side wall of the cylinder (18), and the connecting pipe (20) is connected to the placement chamber (8) at one end away from the cylinder (18); The support plate (4) is provided with a blocking component, through which the through slot (12) is placed in an open or closed state. The blocking component includes a movable block (29). A sliding cavity (30) is horizontally opened in the middle of the through slot (12). The movable block (29) is slidably arranged in the sliding cavity (30). A spring (26) for returning the movable block (29) is fixed inside the sliding cavity (30). A magnetic block (28) is fixed at the bottom end of the movable rod (11). The magnetic block (28) attracts the movable block (29) to slide along the sliding cavity (30) through magnetic force.

2. The storage device for maintaining the biomechanical strength of bone tissue according to claim 1, characterized in that: A second through slot (34) is provided in the middle of the movable block (29), a retaining ring (32) is fixed in the middle of the second through slot (34), a flap (33) is fitted above the retaining ring (32), and the flap (33) is rotatably mounted on the inner wall of the second through slot (34) via a torsion spring.

3. The storage device for maintaining the biomechanical strength of bone tissue according to claim 2, characterized in that: The lifting assembly is provided with a pressure relief assembly, and the pressure relief assembly includes an air outlet (27) provided at the top of the cylinder (18); an installation cavity is provided inside the piston plate (21); through holes are provided on the upper and lower sides of the installation cavity; a movable plate (24) is slidably installed in the middle of the installation cavity; a push rod (25) is fixed on the top of the movable plate (24); the push rod (25) extends to the top of the piston plate (21) through the through hole; a spring 2 (31) is provided below the movable plate (24); the movable plate (24) is pushed upward by the spring 2 (31) to block the through hole above.

4. The storage device for maintaining the biomechanical strength of bone tissue according to claim 3, characterized in that: A limiting ring (5) is fixed to the upper inner wall of the storage tank (1).

5. The storage device for maintaining the biomechanical strength of bone tissue according to claim 4, characterized in that: A vertical rod (35) is slidably installed in the vertical groove opened in the middle of the connecting rod (19), and a push block (37) is slidably installed in the horizontal groove opened in the middle of the piston plate (21). The push block (37) is formed by combining two right-angled trapezoidal blocks, and a spring (36) is installed inside the horizontal groove for driving the push block (37) to move horizontally. The top end of the vertical rod (35) extends to above the top plate (23), and the bottom end of the vertical rod (35) extends to the inside of the horizontal groove and abuts against the inclined surface of the left trapezoidal block in the push block (37). The movable plate (24) is provided with a truncated cone shape, and the inclined surface of the right trapezoidal block of the push block (37) is adapted to the inclined surface of the movable plate (24).

6. The storage device for maintaining the biomechanical strength of bone tissue according to claim 5, characterized in that: A threaded hole is provided in the middle of the sealing cover (2), a threaded rod (16) is threadedly connected to the threaded hole, a pressure plate (17) is rotatably mounted on the bottom end of the threaded rod (16), and a turning handle (15) is fixed on the top end of the threaded rod (16).

7. The storage device for maintaining the biomechanical strength of bone tissue according to claim 6, characterized in that: A sealing ring (14) is fixed on the lower end surface of the limiting ring (5) and located at the edge of the center hole.

8. A method for maintaining the biomechanical strength of bone tissue, the method using the device for maintaining the biomechanical strength of bone tissue according to claim 7, characterized in that: The following steps are involved: S1. Place the bone tissue into a preservation bucket (7) filled with biological medium; S2, the motor drives the screw (10) to rotate, pushing the movable rod (11) to move downward along the sleeve rod (13), thereby driving the sealing ring (6) to move downward to squeeze the low-temperature gas below, and introducing the low-temperature gas inside the placement chamber (8) into the cylinder (18) through the connecting pipe (20), emptying the low-temperature gas in the placement chamber (8), and pushing the piston plate (21), the connecting rod (19) and the movable plate (24) to move upward; S3, in step S2, during the downward movement of the movable rod (11), the driving magnetic block (28) and the movable block (29) are displaced, and the movable block (29) is pushed to move by the spring and close the through slot, so that the cold air enters the interior of the cylinder (18) through the connecting pipe (20); S4, the sealing ring (6) continues to move downward until it fits with the support plate (4), during which the excess cold air inside the cylinder (18) is discharged into the liquid nitrogen chamber (9) through the pressure relief assembly; S5. Open the sealing cover (2) and insert the storage barrel (7) along the center hole of the limiting ring (5) until it contacts the top plate (23); S6, control the motor to reverse, drive the screw rod (10), the movable rod (11) and the sealing ring (6) to move in the opposite direction, generate negative pressure inside the placement chamber (8), draw the low-temperature gas at the bottom of the piston plate (21) into the placement chamber (8), and at the same time drive the flap (33) to rotate upward and open, so that the low-temperature gas in the liquid nitrogen chamber (9) enters the placement chamber (8) through the second through groove (34) in the middle of the movable block (29); S7. After the sealing plate moves up to the initial position, the movable plate (24) moves down to the groove (22) above the support plate (4), the storage barrel (7) is completely received in the placement cavity (8), the sealing cover (2) is installed, and finally the pressing plate (17) is driven by rotating the threaded rod (16) to press on the top of the storage barrel (7), and the storage work is completed.

Images