Fresh cut flower preservation capsule and preparation method thereof

Abstract

The invention relates to a fresh cut flower preservation capsule and a preparation method thereof, and belongs to the technical field of flower preservation. The fresh-keeping capsule is prepared by the following steps: preparing particles from 0.3-1% of penicillin or cephalosporin, 0.1-1% of tetramycin or 8-hydroxyquinoline, 1.5-3% of alum or ferrous sulfate and pelelith or bentonite which are supplemented to 100%, and wrapping the particles with a semipermeable membrane to prepare the capsule. The obtained capsule is convenient to use, and the reproduction and growth of bacteria and fungi in water are better inhibited through the synergistic effect of the active components; the water quality is kept clear through a flocculating agent; the action time of the fresh-keeping capsule is prolonged through the slow-release agent and the semipermeable membrane, so that the fresh-keeping period of the fresh flowers reaches 12-15 days.

Description

Technical Field

[0001] The present invention belongs to the technical field of flower preservation, and particularly relates to an environment-friendly antibacterial sustained-release capsule with agricultural antibiotics as the main active ingredient and a preparation method thereof. Background Art

[0002] In the field of fresh cut flower preservation, substances with functions such as inhibiting bacterial growth, supplementing nutrients, inhibiting ethylene synthesis, and inhibiting water transpiration are usually added to the preservation solution to extend the preservation period of fresh flowers. The main components of these preservation solutions include antibacterial agents, saccharide substances, growth regulators, and ethylene inhibitors. However, despite the wide variety of preservation solution products on the market, their actual application in flower shop retail and personal household use is restricted by many factors. The main problems are their high cost and inconvenient use. Take a flower shop as an example. If there are 30 buckets for storing fresh cut flowers, about 450 liters of preservation solution need to be prepared. As the flower materials continuously absorb the preservation solution, its concentration gradually decreases, and the antibacterial effect also weakens accordingly, resulting in the need for frequent replacement of the preservation solution. The preparation and replacement of a large amount of preservation solution not only consume a large amount of funds but also occupy a large amount of manual time. Therefore, the dual pressures of cost and labor input greatly limit the widespread application of preservation solutions in fresh cut flower preservation.

[0003] In addition, the main components of preservation solutions are mostly chemical components and saccharides. Although some chemical preservatives have good preservation effects, there may be problems of excessive toxicity, which pose potential hazards to the environment and human health in the long term; while saccharide-based preservation solutions are prone to microbial growth, leading to stem rot, and the preservation period usually does not exceed 5 days.

[0004] In addition to the aforementioned preservation methods, the prior art also has a refrigeration preservation method, but the refrigeration method has high energy consumption, is prone to damage the cell membrane of flower petals, and is not suitable for flower shop retail and personal household use.

[0005] Therefore, it is of great significance to develop a fresh cut flower preservation technology that is simple to use, low in cost, environmentally friendly, and has a significant preservation effect. Summary of the Invention

[0006] The present invention aims to provide a fresh cut flower preservation capsule and a preparation method thereof to solve the technical problems existing in the aforementioned prior art.

[0007] To solve the aforementioned technical problems, the present invention provides the following technical solutions:

[0008] A fresh cut flower preservation capsule is made by forming a granule from an antibacterial drug, a flocculant, and a sustained-release agent, and then wrapping it with a semi-permeable membrane; the mass ratio of the antibacterial drug to the flocculant is 4 - 20:15 - 30.

[0009] The antibacterial agent is a mixture of a β-lactam antibiotic and other low-toxicity antibacterial agents, and the mass ratio of the β-lactam antibiotic to the other low-toxicity antibacterial agents is 3-10:1-10.

[0010] The β-lactam antibiotic is penicillin or cephalosporin; the other low-toxicity antibacterial agent is tetramycin or 8-hydroxyquinoline.

[0011] The cephalosporin is cefpirome.

[0012] The flocculant is alum or ferrous sulfate.

[0013] The slow-release agent is volcanic rock or bentonite.

[0014] The semi-permeable membrane is a polyvinyl alcohol membrane or a sodium alginate membrane.

[0015] The formulation of the granule is: 0.3-1% of β-lactam antibiotic, 0.1-1% of other low-toxicity antibacterial agents, 1.5-3% of flocculant, and the slow-release agent makes up 100%.

[0016] The present invention also claims to protect the preparation method of the aforementioned fresh cut flower preservation capsule, which includes the following steps: First, crush the antibacterial agent and the flocculant, then mix them with the slow-release material to form granules, and finally wrap the granules with a semi-permeable membrane to make a capsule.

[0017] The length of the granule is 2-4 cm, and the diameter is 0.5-2 cm.

[0018] The preparation method of the fresh cut flower preservation capsule described in the present invention is simple and convenient to use. The antibacterial agent therein, as the main active ingredient, is used to inhibit the reproduction and growth of bacteria and fungi in water, reduce the invasion of bacteria on the stems of fresh cut flowers, and has a synergistic effect; the flocculant is used to settle impurities and suspended substances in water, prevent water turbidity, and keep the water quality clear; the slow-release agent is used to control the release rate of the preservation components; the semi-permeable membrane is used to wrap the preservation components to further extend the action time of the preservation capsule, so as to achieve a fresh flower preservation period of more than 10 days.

[0019] Verified by experiments, the fresh cut flower preservation capsule described in the present invention has a significant preservation effect. The vase life of cut roses reaches 12-15 days at 25°C (the blank control group is only about 6 days); the maintenance cost is significantly reduced. Taking a 100-square-meter flower shop as an example, the labor cost is reduced by 6 hours / person / day. Detailed implementation mode

[0020] The innovative motivation of the present invention lies in solving problems existing in the existing fresh cut flower preservation technologies, such as the excessive toxicity of chemical preservatives, the fast spoilage rate of sucrose-based preservatives, the inability of existing products to simultaneously inhibit ethylene production and relieve vascular bundle blockage, the cumbersome use, high cost, and the need for a large amount of labor. The aim is to invent a preservation method that is simple to use, convenient to carry, and low in cost.

[0021] Therefore, the inventor designed and conducted the following experiments: (Cefepime used in the embodiments of the present invention is Cefpirome).

[0022] I. Determination of effective active substances and ratios

[0023] 1. Design of experimental groups

[0024] Control group 1: Blank.

[0025] Control group 2: 100 ml of commercially available Chrysal (strengthened type).

[0026] Control group 3: 200 ml of commercially available Chrysal (strengthened type).

[0027] Mixture group 1: 0.3 g of penicillin, 0.1 g of 8-hydroxyquinoline, and 2 g of alum.

[0028] Mixture group 2: 0.3 g of penicillin, 0.5 g of 8-hydroxyquinoline, and 2 g of alum.

[0029] Mixture group 3: 0.3 g of penicillin, 1 g of 8-hydroxyquinoline, and 2 g of alum.

[0030] Mixture group 4: 0.3 g of penicillin, 2 g of 8-hydroxyquinoline, and 2 g of alum.

[0031] Mixture group 5: 1 g of penicillin, 0.3 g of 8-hydroxyquinoline, and 2 g of alum.

[0032] Mixture group 6: 2 g of penicillin, 0.1 g of 8-hydroxyquinoline, and 2 g of alum.

[0033] Mixture group 7: 0.3 g of penicillin, 0.1 g of tetramycin, and 2 g of alum.

[0034] Mixture group 8: 0.3 g of penicillin, 0.5 g of tetramycin, and 2 g of alum.

[0035] Mixture group 9: 0.3 g of penicillin, 1 g of tetramycin, and 2 g of alum.

[0036] Mixture group 10: 0.3 g of penicillin, 2 g of tetramycin, and 2 g of alum.

[0037] Mixture group 11: 1 g of penicillin, 0.3 g of tetramycin, and 2 g of alum.

[0038] Mixture Group 12: 2 g of penicillin, 0.1 g of tetramycin, 2 g of alum.

[0039] Mixture Group 13: 0.3 g of cephalosporin, 0.1 g of 8-hydroxyquinoline, 1.5 g of ferrous sulfate.

[0040] Mixture Group 14: 0.3 g of cephalosporin, 0.5 g of 8-hydroxyquinoline, 1.5 g of ferrous sulfate.

[0041] Mixture Group 15: 0.3 g of cephalosporin, 1 g of 8-hydroxyquinoline, 1.5 g of ferrous sulfate. Mixture Group 16: 0.3 g of cephalosporin, 2 g of 8-hydroxyquinoline, 1.5 g of ferrous sulfate. Mixture Group 17: 1 g of cephalosporin, 0.3 g of 8-hydroxyquinoline, 1.5 g of ferrous sulfate. Mixture Group 18: 2 g of cephalosporin, 0.1 g of 8-hydroxyquinoline, 1.5 g of ferrous sulfate. Mixture Group 19: 0.3 g of cephalosporin, 0.1 g of tetramycin, 1.5 g of ferrous sulfate. Mixture Group 20: 0.3 g of cephalosporin, 0.5 g of tetramycin, 1.5 g of ferrous sulfate. Mixture Group 21: 0.3 g of cephalosporin, 1 g of tetramycin, 1.5 g of ferrous sulfate.

[0042] Mixture Group 22: 0.3 g of cephalosporin, 2 g of tetramycin, 1.5 g of ferrous sulfate.

[0043] Mixture Group 23: 1 g of cephalosporin, 0.3 g of tetramycin, 1.5 g of ferrous sulfate.

[0044] Mixture Group 24: 2 g of cephalosporin, 0.1 g of tetramycin, 1.5 g of ferrous sulfate.

[0045] Single Agent Group 1: 1 g of penicillin, 2 g of alum.

[0046] Single Agent Group 2: 1 g of 8-hydroxyquinoline, 2 g of alum.

[0047] Single Agent Group 3: 1 g of cephalosporin, 1.5 g of ferrous sulfate.

[0048] Single Agent Group 4: 1 g of tetramycin, 1.5 g of ferrous sulfate.

[0049] 2. Test Method

[0050] Except for Control Group 1, put the aforementioned test groups into 10 L of clean water. After stirring evenly, pour the 10 L of liquid into 3 small buckets on average (i.e., 3 replicates for each test group). Control Group 1 is a blank control and no substances need to be added. Keep the test environment temperature at 25°C uniformly. Select fresh-cut roses at the same time. Insert 10 roses into each small bucket. Re-prepare the test solution and change the liquid every 3 days. Control Group 1 changes the clean water. Observe the time when each petal starts to wilt, droop, or fall off. Take the average value (rounded to an integer) of the results of 30 flowers in each test group as the ornamental period.

[0051] 3. Test Results

[0052] Experimental group Ornamental period: days Experimental group Ornamental period: days Control group 1 6 / / Control group 2 9 Mixture group 14 13 Control group 3 10 Mixture group 15 13 Mixture group 1 13 Mixture group 16 13 Mixture group 2 14 Mixture group 17 13 Mixture group 3 12 Mixture group 18 12 Mixture group 4 11 Mixture group 19 12 Mixture group 5 13 Mixture group 20 13 Mixture group 6 10 Mixture group 21 14 Mixture group 7 13 Mixture group 22 11 Mixture group 8 13 Mixture group 23 14 Mixture group 9 12 Mixture group 24 10 Mixture group 10 9 Single agent group 1 8 Mixture group 11 12 Single agent group 2 8 Mixture group 12 11 Single agent group 3 8 Mixture group 13 13 Single agent group 4 9

[0053] As can be seen from the above table, the fresh-keeping effects of Control Group 2, all mixture groups, and all single-agent groups are better than those of Control Group 1 (water control). Except for Mixture Group 10, the fresh-keeping effects of other mixture groups are better than those of the single-agent groups and Control Group 2. In addition, by visual observation, the maintenance of water clarity by the mixture groups and single-agent groups is significantly better than that of Control Group 1 and Control Group 2.

[0054] II. Determination of Preparations

[0055] 1. Design of Test Groups

[0056] Powder 1: 0.3 g of penicillin, 0.5 g of 8-hydroxyquinoline, and 2 g of alum are mixed and pulverized to form a powder.

[0057] Powder 2: 0.3 g of cephalosporin, 1 g of tetramycin, and 1.5 g of ferrous sulfate are mixed and pulverized to form a powder.

[0058] Granule 1: 0.3 g of penicillin, 0.5 g of 8-hydroxyquinoline, 2 g of alum, and volcanic stone are added to make up 100 g, and granules with a length of 2 cm and a diameter of 1 cm are made.

[0059] Granule 2: 0.3 g of cephalosporin, 1 g of tetramycin, 1.5 g of ferrous sulfate, and bentonite are added to make up 100 g, and granules with a length of 2 cm and a diameter of 1 cm are made.

[0060] Capsule 1: The aforementioned Granule 1 is made into a capsule using a polyvinyl alcohol film.

[0061] Capsule 2: The aforementioned Granule 2 is made into a capsule using a sodium alginate film.

[0062] 2. Test Methods

[0063] Prepare 6 buckets of water, add 10 L of clear water to each bucket, and separately put the aforementioned obtained Powder 1, Powder 2, Granule 1, Granule 2, Capsule 1, and Capsule 2 into each bucket of clear water. Keep the test environment temperature at 25°C uniformly. Select roses cut at the same time, and insert 30 roses into each bucket. Change the liquid once every 6 days. Observe the time when each petal begins to wilt, droop, or fall off, and take the average value (rounded to an integer) of the results of 30 flowers in each test group as the ornamental period.

[0064] 3. Test Results

[0065] Experimental group Ornamental period: days Experimental group Ornamental period: days Powder 1 10 Powder 2 11 Granule 1 13 Granule 2 13 Capsule 1 15 Capsule 2 15

[0066] As can be seen from the above table, the best fresh-keeping effect can be achieved by formulating the fresh-keeping formula into capsules.

Claims

1. A fresh cut flower preservation capsule is made by forming a granule from an antibacterial drug, a flocculant and a sustained release agent, and then wrapping it with a semipermeable membrane; it is characterized in that, The mass ratio of the antibacterial agent to the flocculant is 4 - 20:15 - 30.

2. The fresh cut flower preservation capsule according to claim 1, wherein The antibacterial agent is a mixture of β-lactam antibiotics and other low-toxicity antibacterial agents, and the mass ratio of the β-lactam antibiotics to the other low-toxicity antibacterial agents is 3 - 10:1 - 10.

3. The fresh cut flower preservation capsule according to claim 2, wherein The β-lactam antibiotics are penicillin or cephalosporin; the other low-toxicity antibacterial agents are tetramycin or 8-hydroxyquinoline.

4. The fresh cut flower preservation capsule according to claim 3, characterized in that, The cephalosporin is cefpirome.

5. The fresh cut flower preservation capsule according to claim 3, wherein, The flocculant is alum or ferrous sulfate.

6. The fresh cut flower preservation capsule according to claim 3, wherein, The sustained-release agent is volcanic rock or bentonite.

7. The fresh cut flower preservation capsule according to claim 3, wherein The semi-permeable membrane is a polyvinyl alcohol membrane or a sodium alginate membrane.

8. The fresh cut flower preservation capsule according to claim 3, characterized in that, The formulation of the granule is: 0.3 - 1% of β-lactam antibiotics, 0.1 - 1% of other low-toxicity antibacterial agents, 1.5 - 3% of flocculant, and the sustained-release agent makes up 100%.

9. The preparation method of the fresh cut flower preservation capsule according to any one of claims 1-8, characterized in that, It includes the following steps: First, crush the antibacterial agent and the flocculant, then mix them with the sustained-release material to form granules, and finally wrap the granules with a semi-permeable membrane to make capsules.

10. The preparation method of the fresh cut flower preservation capsule according to claim 9, characterized in that, The length of the granule is 2 - 4 cm, and the diameter is 0.5 - 2 cm.