Pesticide composition and application thereof in prevention and treatment of cucumber root-knot nematode

Abstract

The invention discloses a pesticide composition and application thereof in prevention and treatment of cucumber root-knot nematode, and belongs to the technical field of pesticides. The pesticide composition provided by the invention is composed of a matrine extract, amino-oligosaccharin and an auxiliary agent, a 1.5% matrine extract and amino-oligosaccharin soluble concentrate is prepared, and the mass ratio of the matrine extract to the amino-oligosaccharin is 1: 2. The pesticide composition provided by the invention can improve the effect of preventing and treating cucumber root-knot nematode, has an obvious synergistic effect, and is beneficial to food safety.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition and its application in controlling cucumber root-knot nematodes. Background Art

[0002] Cucumber root-knot nematodes have three stages: eggs, larvae, and adults. Among them, eggs, first-instar larvae, third-instar larvae, fourth-instar larvae, and adults are all non-infectious, and only second-instar larvae are infectious. After cucumber transplantation, the nearby root-knot nematodes begin to infect immediately. The peak period for second-instar larvae of cucumber root-knot nematodes to invade the roots is 5 - 7 days after transplantation. One to two days after invading the roots, root knots begin to form.

[0003] Matrine extract is an alkaloid prepared by ethanol extraction from the roots, stems, leaves, and fruits of Sophora flavescens, containing various alkaloids such as matrine, sophocarpine, sophoridine, albiflorine, and oxysophocarpine. Matrine extract is composed of multiple components with similar chemical structures and multiple components with dissimilar chemical structures. They complement each other and work together. Matrine has the activities of killing nematodes, insects, and bacteria.

[0004] Oligosaccharins are natural polysaccharide products (oligosaccharides with D-glucosamine linked by β-1,4 glycosidic bonds) extracted from the shells of marine organisms such as shrimps and crabs. They are obtained by degrading chitin to chitosan and then further degrading it, or by microbial fermentation and extraction. Different from traditional pesticides, oligosaccharins do not directly act on harmful organisms, but instead stimulate the plant's own immune response, causing the plant to produce disease-resistant substances such as phenolic compounds, chitinase, glucanase, lignin, phytoalexins, and pathogenesis-related proteins, obtaining systemic resistance, and thus playing the roles of stress resistance, disease and pest resistance, and yield increase.

[0005] There are not many registered pesticides for controlling cucumber root-knot nematodes, mainly pesticides such as abamectin and fosthiazate. Long-term use is likely to lead to drug resistance. Therefore, there is an urgent need to develop new pesticides for controlling cucumber root-knot nematodes. Summary of the Invention

[0006] The purpose of the present invention is to provide a pesticide composition and its application in controlling cucumber root-knot nematodes.

[0007] The technical solution of the present invention is as follows:

[0008] A pesticide composition and its application in controlling cucumber root-knot nematodes, the pesticide composition is composed of matrine extract, oligosaccharins, and adjuvants, and is made into a 1.5% matrine extract · oligosaccharins soluble concentrate, wherein the weight ratio of matrine extract to oligosaccharins is 1:2;

[0009] Furthermore, the matrine extract includes matrine and sophocarpine, with matrine being 0.5% and sophocarpine being 0.45%;

[0010] Further, the pesticide registration number of the matrine extract is PD20110637.

[0011] Further, the adjuvant is selected from one or more of surfactants, defoamers, and fillers;

[0012] Further, the surfactant is selected from one or more of Tween-80, Tween-60, fatty alcohol polyoxyethylene ether, polyether polyol, phenethylphenol polyoxyethylene polypropylene ether, polyheptylene glycol monodecyl ether, pentapolyethylene glycol monodecyl ether, tripolyethylene glycol monodecyl ether, alkyldodecanol polyoxyethylene polypropylene ether, and lauryl alcohol polyoxyethylene ether;

[0013] Further, the defoamer is selected from one or more of silicone and fatty alcohol;

[0014] Further, the filler is selected from deionized water.

[0015] Further, the pesticide composition made into a soluble concentrate includes the following components and contents: 5% of 10% matrine extract technical material, 1.18% of 85% oligosaccharins technical material, 3-15% of surfactant, 0.2-0.5% of defoamer, and deionized water is added to 100%, to obtain 1.5% matrine extract·oligosaccharins soluble concentrate.

[0016] Further, the application is used for the control of cucumber root-knot nematodes, by irrigation application, the dosage of the active ingredient is 56-78 g / ha, with an interval of 7 days, and the pesticide is applied 2 times.

[0017] Compared with the prior art, the pesticide composition of the present invention has the following beneficial effects:

[0018] (1) Compared with single agents, the pesticide composition has obvious synergistic effects on cucumber root-knot nematodes and significantly improves the control effect.

[0019] (2) The active ingredients in the pesticide composition are compounded with pesticides having different action mechanisms, which can delay the resistance of pests.

[0020] (3) The active ingredients matrine extract and oligosaccharins in the pesticide composition are both biopesticides and can be used in the production of organic vegetables.

[0021] (4) The added adjuvant in the pesticide composition avoids the flocculation phenomenon of oligosaccharins, ensures the stable quality of the product, and at the same time improves the control effect of the medicament on cucumber root-knot nematodes. Specific embodiments

[0022] The following further illustrates the present invention in conjunction with embodiments. The percentages in the embodiments are all mass fraction ratios, but the present invention is not limited thereto.

[0023] Example 1

[0024] Example 1 1.5% Matrine Extract·Amino Oligosaccharins Solution

[0025] 5% of 10% Matrine Extract Technical, 1.18% of 85% Amino Oligosaccharins Technical, 5% of Tween-80, 5% of Polyheptylene Glycol Monodecyl Ether, 0.2% of Organosilicon, and deionized water q.s. to 100%, to obtain 1.5% Matrine Extract·Amino Oligosaccharins Solution

[0026] Example 2 1.5% Matrine Extract·Amino Oligosaccharins Solution

[0027] 5% of 10% Matrine Extract Technical, 1.18% of 85% Amino Oligosaccharins Technical, 5% of Tween-80, 6% of Lauryl Alcohol Polyoxyethylene Ether, 0.2% of Organosilicon, and deionized water q.s. to 100%, to obtain 1.5% Matrine Extract·Amino Oligosaccharins Solution

[0028] Example 3 1.5% Matrine Extract·Amino Oligosaccharins Solution

[0029] 5% of 10% Matrine Extract Technical, 1.18% of 85% Amino Oligosaccharins Technical, 4% of Polyether Polyol, 5% of Phenylethylphenol Polyoxyethylene Polyoxypropylene Ether, 0.3% of Organosilicon, and deionized water q.s. to 100%, to obtain 1.5% Matrine Extract·Amino Oligosaccharins Solution

[0030] Control Example 1 1.5% Matrine Extract·Amino Oligosaccharins Solution

[0031] 5% of 10% Matrine Extract Technical, 1.18% of 85% Amino Oligosaccharins Technical, 10% of Sodium Dodecyl Sulfate, 0.2% of Organosilicon, and deionized water q.s. to 100%, to obtain 1.5% Matrine Extract·Amino Oligosaccharins Solution

[0032] Control Example 2 1% Amino Oligosaccharins Solution

[0033] 1.18% of 85% Amino Oligosaccharins Technical, 5% of Tween-80, 6% of Lauryl Alcohol Polyoxyethylene Ether, 0.2% of Organosilicon, and deionized water q.s. to 100%, to obtain 1% Amino Oligosaccharins Solution

[0034] Control Example 3 0.5% Matrine Extract Solution

[0035] 5% of 10% Matrine Extract Technical, 5% of Tween-80, 6% of Lauryl Alcohol Polyoxyethylene Ether, 0.2% of Organosilicon, and deionized water q.s. to 100%, to obtain 0.5% Matrine Extract Solution

[0036] Example 2

[0037] Product quality stability test

[0038] The results of the product quality stability test are shown in Table 1.

[0039] Table 1 Product quality test results

[0040]

[0041]

[0042] It can be seen from Table 1 that the test results of Examples 1, 2, and 3 and the heat storage stability test results all meet the product quality indicators, indicating that adding the surfactant screened by the present invention, the product quality is stable. The test results of Comparative Example 1 show that there are visible suspended matters and precipitates in the appearance, the content of aminooligosaccharin is 0.75%, which is lower than the product index, and the dilution stability (diluted 20 times) is unqualified; after heat storage, the decomposition rate of aminooligosaccharin is 18%, and the dilution stability is unqualified. It shows that adding a common surfactant is likely to cause unstable product quality.

[0043] Example Three

[0044] Indoor combined toxicity determination of matrine extract and aminooligosaccharin against Meloidogyne incognita on cucumber

[0045] 1 Test purpose

[0046] By determining and evaluating the toxicity of matrine extract and aminooligosaccharin and their different mixing combinations against Meloidogyne incognita on cucumber, clarify their compatibility, and provide a scientific basis for the research and development of the mixture of matrine extract and aminooligosaccharin.

[0047] 2 Test design

[0048] 2.1 Test agents

[0049] 10% matrine extract technical, purchased from Inner Mongolia Zhongnong Biochemical Technology Co., Ltd.;

[0050] 85% aminooligosaccharin technical, purchased from Qingdao Zhongda Agricultural Technology Co., Ltd.

[0051] 2.2 Agent preparation

[0052] On the basis of the preliminary test, weigh a certain amount of technical or mother drug, dissolve it in water to make a mother liquor. For the composition, first dilute the single-agent mother liquor to the same concentration, and then mix it in a volume ratio of 3:1, 2:1, 1:1, 1:2, and 1:3. Using the gradient dilution method, set 5 series of doses (as follows), and set the treatment with clear water without active ingredients as the blank control.

[0053] Matrine extract (A): 50, 25, 12.5, 6.25, 3.125 mg / L

[0054] Amino-oligosaccharins (B): 50, 25, 12.5, 6.25, 3.125 mg / L

[0055] A:B (3:1): 20, 10, 5, 2.5, 1.25 mg / L

[0056] A:B (2:1): 20, 10, 5, 2.5, 1.25 mg / L

[0057] A:B (1:1): 20, 10, 5, 2.5, 1.25 mg / L

[0058] A:B (1:2): 20, 10, 5, 2.5, 1.25 mg / L

[0059] A:B (1:3): 20, 10, 5, 2.5, 1.25 mg / L

[0060] 3 Test methods

[0061] 3.1 Chemical treatment

[0062] Transplant the test cucumber seedlings into pots filled with new sterilized soil. After transplantation, allow the seedlings to recover for a period of time, and then use a graduated cylinder to irrigate the roots, 200 mL per plant. When irrigating the roots, first treat the blank control, and then treat the test chemicals in the order from low concentration to high concentration. Immediately make a mark after each treatment is completed.

[0063] 3.2 Inoculation

[0064] Inoculation is carried out 24 hours after chemical treatment. Take an appropriate amount of the soil sample brought back to the laboratory, wrap it with gauze, place it on the funnel paper towel, immerse it in clear water, open the water clamp, take 10 mL of the water sample at the bottom, and check it under a biological microscope. Dilute the nematode suspension concentration to 400 nematodes / mL. Take 5 mL of the nematode suspension and inoculate it into each potted plant of the treatment.

[0065] 3.3 Replication

[0066] Each replication has 10 pots, and each treatment is replicated 4 times.

[0067] 4 Result statistics

[0068] After the test materials are treated and inoculated, transfer them to the greenhouse for normal cultivation and management, and conduct a disease investigation 30 days later.

[0069] Grading standard:

[0070] Grade 0: The roots are intact and there are no root knots;

[0071] Grade 1: A small number of root knots, accounting for less than 25% of the total roots;

[0072] Level 3: A medium number of root knots are formed, accounting for 26 - 50% of the total root system;

[0073] Level 5: There are relatively many root knots, accounting for 51 - 75% of the root system;

[0074] Level 7: There are many and relatively large root knots, accounting for 76 - 100% of the total root system.

[0075] Calculation of drug efficacy:

[0076]

[0077] Perform regression analysis on the results, and use DPS software to obtain the virulence equation and EC 50 .

[0078] 5 Evaluation of synergistic effect

[0079] Adopt the method of calculating the co-toxicity coefficient by Sun Yunpei to calculate the co-toxicity coefficient (CTC) of the mixture. Evaluate the synergistic effect of the mixed agents according to the co-toxicity coefficient (CTC), that is, CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect.

[0080] 6 Experimental results

[0081] Table 2 Indoor combined virulence results of matrine extract and oligosaccharins against cucumber root-knot nematodes

[0082]

[0083]

[0084] It can be seen from Table 2 of the test results that the test agents matrine extract and oligosaccharins have good virulence against cucumber root-knot nematodes. The EC 50 value of matrine extract is 9.003 mg / L, and the EC 50 value of oligosaccharins is 10.296 mg / L; the EC 50 values of the two agents mixed in different proportions against cucumber root-knot nematodes are 10.597, 9.778, 8.904, 7.3945 and 8.052 mg / L respectively, and the co-toxicity coefficients CTC reach 87.71, 96.10, 107.89, 132.88 and 123.44 respectively. It shows that the CTC of matrine extract and oligosaccharins is greater than 120 within the range of 1:2 to 1:3 ratio, and there is a synergistic effect to varying degrees. Among them, the co-toxicity coefficient (CTC) of the 1:2 ratio is the largest, and the synergistic effect is significant.

[0085] Example 4

[0086] Example Field efficacy test for controlling cucumber root-knot nematodes

[0087] 1. Purpose of the experiment

[0088] To verify the efficacy of the 1.5% matrine extract·amino oligosaccharide soluble solution developed by Shaanxi Kanghelifeng Biotechnology Pharmaceutical Co., Ltd. on the control of cucumber root-knot nematodes and its safety to cucumbers, to clarify the field dosage and application technology, and to provide a scientific basis for pesticide registration.

[0089] 2 Test basis

[0090] "Guidelines for Field Efficacy Tests for Pesticide Registration (I) Nematicides for Control of Root Nematodes" (GB / T 17980.38-2000).

[0091] 3 Experimental location

[0092] Jintuo Village, Langli Street, Changsha County, Changsha City, Hunan Province.

[0093] 4. Selection of experimental subjects, crops and varieties

[0094] Test object: cucumber root-knot nematode; test crop: cucumber, variety Suyan No. 6.

[0095] 5 Experimental design and arrangement

[0096] 5.1 Experimental drug design

[0097] Table 3 Experimental design of test drugs

[0098]

[0099] 5.2 Cell Arrangement

[0100] Each treatment was repeated 4 times and the plots were arranged in random blocks.

[0101] 5.3 Application time and frequency

[0102] Root irrigation treatment began before the occurrence of cucumber root-knot nematodes on the day of cucumber transplanting. The first application of the test and control substances was on April 25, 2023 (the cucumbers were in the seedling stage), and the second application of the test and control substances was on May 2, 2023 (the cucumbers were in the seedling stage), for a total of 2 applications.

[0103] 5.4 Usage capacity

[0104] Use 200 ml of water per plant.

[0105] 5.5 Survey time and frequency

[0106] The number of diseased plants at each level was investigated 30 days and 60 days after application of the medicine, the original data was recorded, and the disease index and prevention effect were calculated.

[0107] 5.6 Investigation method

[0108] Take five-point sampling along the diagonal of each plot, investigate 4 plants at each point, record the total number of plants, the number of diseased plants at each level, and calculate the disease index and control effect.

[0109] Grade 0: No galls on the roots;

[0110] Grade 1: A small number of small galls on the roots;

[0111] Grade 3: Two-thirds of the roots are covered with small galls;

[0112] Grade 5: The roots are covered with small galls and have secondary galls;

[0113] Grade 7: The roots form a fibrous root mass.

[0114] 5.7 Method for calculating drug efficacy

[0115]

[0116] 6 Test results

[0117] Table 4 Field drug efficacy test results of the examples for controlling cucumber root-knot nematodes

[0118]

[0119]

[0120] As can be seen from Table 4, in Examples 1, 2, and 3, compared with Comparative Examples 1, 2, and 3, irrigation was carried out at the beginning of the occurrence of cucumber root-knot nematodes, and the drug was applied once every 7 days for a total of 2 times. Under the test drugs, there was a good control effect on cucumber root-knot nematodes.

[0121] 30 days after the last dose of the test substance, the control effects of the treatments with the active ingredient dosages of 56, 67, and 78 g / ha in Examples 1, 2, and 3 on cucumber root-knot nematodes were 74.98%-84.14%. The control effects of the treatments with the active ingredient dosages of 56, 67, and 78 g / ha in Comparative Example 1 on cucumber root-knot nematodes were 66.41%-71.59%. The control effects of the treatments with the active ingredient dosages of 83 and 46 g / ha in Comparative Examples 2 and 3 on cucumber root-knot nematodes were 65.65% and 66.67% respectively.

[0122] 60 days after the last dose of the test substance, the control effects of the treatments with 56, 67, and 78 g / ha of the active ingredients in Examples 1, 2, and 3 on cucumber root-knot nematodes were 71.07%-79.37%. The control effects of the treatments with 56, 67, and 78 g / ha of the active ingredients in Comparative Example 1 on cucumber root-knot nematodes were 64.23%-69.59%. The control effects of the treatments with 83 and 46 g / ha of the active ingredients in Comparative Example 2 and Comparative Example 3 on cucumber root-knot nematodes were 62.03% and 63.28% respectively.

[0123] 30 days and 60 days after the last dose of the test substance, the control effects of Examples 1, 2, and 3 on cucumber root-knot nematodes were significantly higher than those of Comparative Example 1, Comparative Example 2, and Comparative Example 3.

[0124] During the whole test process, no phytotoxicity was found on the leaves, fruits, and plants of cucumbers after applying the test agents, nor were any adverse effects found on the surrounding environment and non-target organisms.

[0125] In summary, the 1.5% matrine extract·oligosaccharin soluble solution can be used for irrigation treatment starting from the day of cucumber transplantation before the occurrence of cucumber root-knot nematodes, which can effectively control cucumber root-knot nematodes. It is recommended to use 56-78 g / ha of the active ingredient of the 1.5% matrine extract·oligosaccharin soluble solution, with an interval of 7 days and a total of 2 applications.

Claims

1. A pesticide composition and its application in controlling root-knot nematodes of cucumbers, characterized in that, The pesticide composition is composed of matrine extract, oligochitosan, and adjuvants, and is made into a 1.5% matrine extract·oligochitosan soluble concentrate. The weight ratio of matrine extract to oligochitosan is 1:2; Furthermore, the matrine extract includes matrine and sophoridine, with matrine at 0.5% and sophoridine at 0.45%; Furthermore, the pesticide registration number of the matrine extract is PD20110637. Furthermore, the adjuvants are selected from one or more of surfactants, defoamers, and fillers; Furthermore, the surfactants are selected from one or more of Tween-80, Tween-60, fatty alcohol polyoxyethylene ether, polyether polyol, phenethylphenol polyoxyethylene polyoxypropylene ether, polyheptylene glycol monodecyl ether, pentapolyethylene glycol monodecyl ether, tripolyethylene glycol monodecyl ether, alkyldodecanol polyoxyethylene polyoxypropylene ether, and lauryl alcohol polyoxyethylene ether; Furthermore, the defoamers are selected from one or more of silicone and fatty alcohol; Furthermore, the filler is selected from deionized water.

2. The pesticidal composition according to claim 1, wherein: The pesticide composition includes the following components and contents: 5% of 10% matrine extract technical, 1.18% of 85% oligochitosan technical, 5% of Tween-80, 5% of polyheptylene glycol monodecyl ether, 0.2% of silicone, and deionized water added to 100%, to obtain a 1.5% matrine extract·oligochitosan soluble concentrate.

3. The pesticide composition according to claim 1, characterized in that: The pesticide composition includes the following components and contents: 5% of 10% matrine extract technical, 1.18% of 85% oligochitosan technical, 5% of Tween-80, 6% of lauryl alcohol polyoxyethylene ether, 0.2% of silicone, and deionized water added to 100%, to obtain a 1.5% matrine extract·oligochitosan soluble concentrate.

4. The pesticide composition according to claim 1, wherein: The pesticide composition includes the following components and contents: 5% of 10% matrine extract technical, 1.18% of 85% oligochitosan technical, 4% of polyether polyol, 5% of phenethylphenol polyoxyethylene polyoxypropylene ether, 0.3% of silicone, and deionized water added to 100%, to obtain a 1.5% matrine extract·oligochitosan soluble concentrate.

5. Use according to claim 1 in preventing and controlling root-knot nematodes of cucumbers, characterized in that: For root irrigation, the dosage of the active ingredient is 56 - 78 grams per hectare, with an interval of 7 days and 2 applications.

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