Composite enzymatic hydrolysis meat and milk mixture, preparation method and application in pet food

Abstract

The invention discloses a composite enzymatic hydrolysis meat-milk mixture, a preparation method thereof and application of the composite enzymatic hydrolysis meat-milk mixture in pet food, and belongs to the technical field of pet food processing. The pet food is cat food and comprises the following components in parts by mass: 40-60 parts of enzymolysis animal protein, 10-20 parts of corn, 10-20 parts of rice, 5-10 parts of enzymolysis dairy products, 10 parts of chicken oil, 4 parts of beef tallow, 2-3 parts of beer yeast powder, 1-2 parts of enzymolysis chicken liver powder, 1-5 parts of compound vitamin minerals, 1-2 parts of vegetable and fruit bags, 0.5-1.5 parts of algae and products thereof and 0.5-2 parts of other additives. The animal protein and the dairy product are subjected to enzymolysis by utilizing a two-stage gradient enzymolysis technology, so that the animal protein and the dairy product are used as raw materials of cat food in the whole life stage, and then are mixed with other components and extruded to obtain the pet food, so that the digestion performance can be obviously improved, and the palatability and the excrement score improvement rate are improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of pet food processing, and particularly relates to a composite enzymatically hydrolyzed meat and milk mixture, a preparation method thereof, and an application thereof in pet food. Background Art

[0002] Disclosing the information of this background art section is only intended to enhance the overall understanding of the present invention, and it is not necessarily regarded as an admission or an implication in any form that this information constitutes the prior art already known to those of ordinary skill in the art.

[0003] With the increase in the number of pets raised, the market demand for pet food is constantly expanding. However, traditional cat food has certain limitations in terms of raw materials and processing technology. Some kittens are prone to problems such as indigestion and intestinal discomfort after eating. Research shows that due to the different nutritional requirements of kittens and adult cats, the nutritional requirements in the kitten stage are higher than those in the adult stage. Just after leaving the mother's body, they have low immunity, are prone to illness, and their digestive systems are not yet perfect. The activity of trypsin secreted by the pancreas is significantly lower than that of adult cats, only 30 - 40% of that of adult cats. And the intestinal health of pets is closely related to the digestion and absorption rates of proteins and fats in their diets. Therefore, it is of great significance to develop a dry food that is nutritionally balanced, comprehensive, and can improve and suit the digestive performance of cats at all life stages.

[0004] Enzymatic hydrolysis technology is a method of decomposing macromolecular substances into small molecular substances through the action of enzymes, which can improve the digestibility of proteins and fats. Fresh meat is rich in high-quality proteins and essential amino acids and is an ideal raw material for pet food; dairy products, as a food with comprehensive nutrition, good palatability, and therapeutic and health care functions, are also indispensable in pet food. At present, in pet food processing, protein raw materials and dairy products are directly added, resulting in low digestibility. How to combine enzymatic hydrolysis technology with animal proteins and dairy products and apply them to pet dry food has not yet had a mature technical solution. Summary of the Invention

[0005] In order to solve the deficiencies of the prior art, the purpose of the present invention is to provide a composite enzymatically hydrolyzed meat and milk mixture, a preparation method thereof, and an application thereof in pet food. The present invention uses a two-stage gradient enzymatic hydrolysis technology to enzymatically hydrolyze animal proteins and dairy products and make them into raw materials for cat food at all life stages.

[0006] In order to achieve the above purpose, the technical solution of the present invention is as follows:

[0007] In a first aspect, the present invention provides a composite enzymatically hydrolyzed meat and milk mixture. By mass fraction, the composite enzymatically hydrolyzed meat and milk mixture comprises: 40 - 60 parts of enzymatically hydrolyzed animal protein and 5 - 10 parts of enzymatically hydrolyzed dairy product; wherein, the enzymatically hydrolyzed animal protein comprises 30 - 40 parts of enzymatically hydrolyzed chicken and 10 - 20 parts of enzymatically hydrolyzed salmon; the composite enzymatically hydrolyzed meat and milk mixture is obtained by subjecting animal protein and dairy product to two-stage enzymatic hydrolysis after adding biological enzymes.

[0008] The biological enzymes include protease, lactase, lipase, flavor protease, and trypsin. Among them, the protease is an animal protein hydrolase, and the lactase is a neutral lactase.

[0009] The functions of various enzymes are different. The animal protein hydrolase is an enzyme preparation mainly for hydrolyzing animal protein, which can hydrolyze animal protein into small molecule peptides and amino acids; lactase can catalyze the decomposition of lactose into one molecule of glucose and one molecule of galactose, facilitating digestion and absorption; lipase can hydrolyze triacylglycerol esters, releasing diglycerides, monoglycerides, glycerol, and free fatty acids, releasing precursors of aroma substances and enhancing the flavor; trypsin has a strong ability to decompose protein, fat, and starch, and can further hydrolyze animal protein and fat; flavor enzyme can further remove bitter peptide chains of low hydrolysis degree products and completely degrade them into amino acids, improving and enhancing the flavor of the hydrolyzate.

[0010] In one or more embodiments, the salmon is pink salmon.

[0011] In one or more embodiments, the dairy product is goat whey powder or whole-fat goat milk powder.

[0012] In a second aspect, the present invention provides a preparation method of the above-mentioned composite enzymatically hydrolyzed meat and milk mixture, comprising the following steps:

[0013] (1) Raw material pretreatment: The frozen chicken and salmon meat are broken into pieces and coarsely ground into granules, and both are added to an enzymatic hydrolysis device and stirred.

[0014] (2) Enzymatic hydrolysis reaction: Two-stage enzymatic hydrolysis is adopted. In the first stage, protease, lactase, and lipase are added for enzymatic hydrolysis; in the second stage, flavor protease and trypsin are added for enzymatic hydrolysis, so that the degree of hydrolysis reaches 35% - 40%, and the proportion of molecules with a molecular weight < 1000 Da exceeds 65%.

[0015] The present invention adopts staged enzymatic hydrolysis. In the first stage, the animal protein, lactose in the dairy product, and fat in the meat and milk are enzymatically hydrolyzed at a temperature suitable for the activities of protease, lactase, and lipase. In the second stage, the small molecules are further enzymatically hydrolyzed at a temperature suitable for the activities of flavor protease and trypsin, while improving and enhancing the flavor of the hydrolyzate.

[0016] In one or more embodiments, in step (1), the slow freezing temperature is -4°C to 0°C, the particle size of the coarsely ground particles is 3 - 6 mm, and the stirring time is 5 - 15 min.

[0017] Specifically: Both chicken and salmon are slowly frozen to -4°C to 0°C, broken into pieces, coarsely ground into 3 - 6 mm particles and then transported to an enzymatic hydrolysis tank. Goat whey powder is added to the enzymatic hydrolysis tank, and stirring and homogenization are started, with an average of 10 min of homogenization and stirring per ton.

[0018] In one or more embodiments, in step (2), in the first stage, at natural pH, enzymatic hydrolysis is carried out at 50 - 60°C for 2 - 4 h, preferably 2 - 2.5 h. In the second stage, at natural pH, enzymatic hydrolysis is carried out at 40 - 50°C for 1 - 3 h, preferably 1 - 1.5 h. The enzymatic hydrolysis temperatures in the first stage and the second stage are different.

[0019] In one or more embodiments, in step (2), in the first stage, protease is added at 1×10 4 U / KG to 3×10 4 U / KG, lactase is added at 4×10 3 U / KG to 6×

[0020] 10 4 U / KG, and lipase is added at 0.5×10 4 U / KG to 1.5×10 4 U / KG; in the second stage, flavor protease is added at 1×10 4 U / KG to 3×10 4 U / KG, and trypsin is added at 1×10 4 U / KG to 3×10 4 U / KG.

[0021] Thirdly, the present invention provides the application of the above-mentioned composite enzymatically hydrolyzed meat and milk mixture in the preparation of pet food. The pet food is cat food.

[0022] Fourthly, the present invention provides a cat food for all life stages based on the composite enzymatically hydrolyzed meat and milk mixture, which adopts the above-mentioned composite enzymatically hydrolyzed meat and milk mixture. The cat food for all life stages includes, by mass: 40 - 60 parts of enzymatically hydrolyzed animal protein, 10 - 20 parts of corn, 10 - 20 parts of rice, 5 - 10 parts of enzymatically hydrolyzed dairy products, 5 - 10 parts of chicken fat, 2 - 5 parts of beef fat, 2 - 3 parts of brewer's yeast powder, 1 - 2 parts of enzymatically hydrolyzed chicken liver powder, 1 - 5 parts of composite vitamins and minerals, 1 - 2 parts of vegetable and fruit package, 0.5 - 1.5 parts of algae and its products, and 0.5 - 2 parts of other additives.

[0023] Among them, the enzymatically hydrolyzed animal protein includes 30 - 40 parts of chicken and 10 - 20 parts of salmon.

[0024] In one or more embodiments, 98% of the brewer's yeast powder passes through a 40-mesh sieve.

[0025] In one or more embodiments, 100% of the enzymatically hydrolyzed chicken liver powder passes through a 20-mesh sieve.

[0026] In one or more embodiments, the vegetable and fruit package includes carrot powder and cranberry powder; the mass ratio of carrot powder to cranberry powder is 15 - 25:1.

[0027] The carrot powder and cranberry powder are purchased from the market, either is acceptable, but it is required that 98% passes through a 20-mesh sieve.

[0028] The alga and its product is spirulina powder.

[0029] The other additives include antioxidant, glucosamine hydrochloride, taurine, potassium chloride, sodium chloride, and postbiotics; among them, the mass ratio of antioxidant, glucosamine hydrochloride, taurine, potassium chloride, sodium chloride, and postbiotics is (3 - 5):(4 - 6):(120 - 130):(140 - 160):(290 - 310):(990 - 1010), preferably 4:5:125:150:300:1000.

[0030] The antioxidant is rosemary extract.

[0031] The glucosamine hydrochloride has a CAS number of 66 - 84 - 2 and has a significant effect in maintaining joint health and promoting cartilage repair, etc.

[0032] In a fifth aspect, the present invention provides a preparation method of the cat food for all life stages based on the above composite enzymatically hydrolyzed meat and milk mixture, which uses the above composite enzymatically hydrolyzed meat and milk mixture and includes the following steps:

[0033] According to the proportion, corn and rice are crushed and then mixed evenly with brewer's yeast powder, compound vitamin and mineral, vegetable and fruit package, alga and its product, and other additives to obtain a blended powder. The blended powder is mixed with the composite enzymatically hydrolyzed meat and milk mixture and then subjected to extrusion and puffing granulation treatment. The puffed granules are subjected to gradient drying and dehydration, and then chicken oil, beef tallow, and enzymatically hydrolyzed chicken liver powder are sprayed on in sequence, and then packaged to obtain the product.

[0034] In one or more embodiments, corn and rice are crushed until 80% passes through a 50 - 70-mesh sieve.

[0035] In one or more embodiments, the gradient drying is to dehydrate in stages from 120°C to 90°C until the moisture content ≤ 8%.

[0036] One or some of the above technical solutions have the following advantages or beneficial effects:

[0037] (1) The present invention enzymatically hydrolyzes animal protein and dairy products by a two-stage gradient enzymatic hydrolysis technique to obtain a composite enzymatically hydrolyzed meat and milk mixture, which is used as a raw material and mixed with other components and then extruded and puffed to obtain pet food. This pet food can significantly improve the digestive performance, increase the palatability and the improvement rate of fecal score, making it very suitable for senior cats and young cats, and thus suitable for pets at all life stages.

[0038] (2) After enzymatic hydrolysis of animal protein and dairy products, small molecule proteins and fats are more easily digested and absorbed by young and senior cats, and lactase hydrolyzes lactose in dairy products, reducing the intestinal burden. After testing, the protein digestibility is increased to 89.26%, and the fat digestibility is increased to 93.21%.

[0039] (3) The addition of enzymatically hydrolyzed meat and dairy products after puffing enhances the flavor of the dry food and increases the appetite of pets, thus improving the palatability.

[0040] (4) The fecal score reaches 2.25, reducing the diarrhea rate of cats. Detailed Embodiments

[0041] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in conjunction with specific embodiments.

[0042] The sources of raw materials used in the embodiments are as follows:

[0043] Corn and broken rice in the Feed Raw Material Catalog of the Announcement No. 1773 of the Ministry of Agriculture of the People's Republic of China. The rice shall comply with the standard of "GB / T 1354-2018 Rice", and the corn shall comply with

[0044] the standard of "GB 1353-2018 Corn". All commercially available products meet the requirements.

[0045] The chicken oil and beef tallow are both edible animal fats and comply with the national food safety standard of GB 10146-2015. The chicken oil and beef tallow in the embodiments are all commercially available products.

[0046] The brewer's yeast powder can be of ordinary commercial use.

[0047] The algae and its products are spirulina powder and can be of ordinary commercial use.

[0048] Other additives include antioxidants, glucosamine hydrochloride, taurine, potassium chloride, sodium chloride and postbiotics.

[0049] Among them, glucosamine hydrochloride, taurine, potassium chloride and sodium chloride can be of ordinary commercially available products.

[0050] The antioxidant is rosemary extract (liposoluble), produced by Hunan Jinhan Pharmaceutical Co., Ltd., and the product code is CAO-10.

[0051] The postbiotic is purchased from Shandong Taishan Shengliyuan Group Co., Ltd., and it is the company's product "Postbiotic Treasure". Its raw material composition is fermented with soybean meal and soybean fiber as the matrix, and it is rich in postbiotic components such as heat-inactivated Lactobacillus casei, heat-inactivated Saccharomyces cerevisiae and their metabolites. The guaranteed values of its product component analysis are moisture ≤ 12%, crude protein ≥ 25%, crude ash ≤ 8%, crude fiber ≤ 10%, small peptides ≥ 10%, L-lactic acid ≥ 3.5%, glutamine ≥ 1%, and mannan ≥ 1%.

[0052] The compound vitamin and mineral contains dl-α-tocopheryl acetate, L-ascorbic acid-2-phosphate, nicotinamide, thiamine nitrate (vitamin B1), calcium D-pantothenate, riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), cyanocobalamin (vitamin B12), folic acid, vitamin D3, D-biotin, protein zinc, protein iron, protein copper, protein manganese, sodium selenite. In this product, vitamin E ≥ 12,000 IU / kg, vitamin C ≥ 200,000 mg / kg, nicotinamide ≥ 7,000 mg / kg, vitamin B1 ≥ 10,000 mg / kg, calcium D-pantothenate ≥ 1,000 mg / kg, vitamin B2 ≥ 800 mg / kg, vitamin B6 ≥ 700 mg / kg, vitamin B12 ≥ 10 mg / kg, folic acid ≥ 120 mg / kg, vitamin D3 ≥ 180,000 IU / kg, D-biotin ≥ 25 mg / kg, zinc ≥ 10,000 mg / kg, iron ≥ 10,000 mg / kg, copper ≥ 2,000 mg / kg, manganese ≥ 800 mg / kg, selenium ≥ 36 mg / kg. It is purchased from Shanghai Aini Maowo Biotechnology Co., Ltd.

[0053] The enzymatically hydrolyzed chicken liver powder is purchased from Jiangsu Yichong Biotechnology Co., Ltd., and the product code is CP-3.

[0054] The animal protein hydrolase, neutral lactase, lipase, flavor protease, and trypsin are all purchased from Nanning Pangbo Bioengineering Co., Ltd.

[0055] Example 1

[0056] 1. The preparation method of the compound enzymatically hydrolyzed meat and milk mixture (a mixture of enzymatically hydrolyzed animal protein and dairy products) is as follows:

[0057] (1) Raw material pretreatment:

[0058] 30 parts of chicken meat and 10 parts of salmon meat are slowly frozen to -4°C - 0°C, broken into pieces, coarsely minced into 4 mm particles, and then transported to the enzymolysis tank. 10 parts of sheep milk powder are added to the enzymolysis tank, and stirring and homogenization are started. The average homogenization and stirring time per ton is 10 minutes.

[0059] (2) Enzymatic hydrolysis reaction:

[0060] Two-stage enzymatic hydrolysis is adopted. In the first stage, protease at 2×10 4 U / KG, lactase at 5×10 3 U / KG, and lipase at 1×10 4 U / KG are added. The enzymatic hydrolysis is carried out at natural pH and 55°C for 120 minutes. In the second stage, flavor protease at 2×10 4 U / KG and trypsin at 2×10 4 U / KG are added. The enzymatic hydrolysis is carried out at natural pH and 45°C for 90 minutes (the degree of hydrolysis reaches 35%-40%, and the proportion of molecules with a molecular weight <1000Da exceeds 65%).

[0061] 2. Preparation of cat food using two-stage gradient enzymatic hydrolysis technology to enzymatically hydrolyze animal protein and dairy products as raw materials:

[0062] (1) Prepare various raw materials according to the raw material formula and set aside;

[0063] It is made from the following raw material ratios: 50 parts of the mixture of enzymatically hydrolyzed animal protein and dairy products prepared above, 20 parts of corn, 15 parts of rice, 10 parts of chicken fat, 4 parts of beef fat, 3 parts of brewer's yeast powder, 2 parts of enzymatically hydrolyzed chicken liver powder, 1 part of compound vitamin and mineral, 1 part of vegetable and fruit package, 0.7 part of algae and its products, and 0.5 part of other additives.

[0064] Among them, the compound vitamin and mineral contains dl-α-tocopheryl acetate, L-ascorbic acid-2-phosphate, nicotinamide, vitamin A, thiamine nitrate (vitamin B1), D-calcium pantothenate, riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), cyanocobalamin (vitamin B12), folic acid, vitamin D3, D-biotin, protein zinc, protein iron, protein copper, protein manganese, and sodium selenite.

[0065] The vegetable and fruit package contains carrot powder and cranberry powder, and the mass ratio is 20:1.

[0066] Other additives include antioxidant, glucosamine hydrochloride, taurine, potassium chloride, sodium chloride, and postbiotics, and their mass ratio is 4:5:125:150:300:1000.

[0067] The antioxidant is rosemary extract.

[0068] The algae and its products are spirulina powder.

[0069] (2) Crush corn and rice to pass through a 60-mesh sieve with 80% passing rate, and mix them evenly with brewer's yeast powder, compound vitamin and mineral, vegetable and fruit package, algae and its products, and other additives to make the ingredient powder for later use;

[0070] (3) Mix the mixture of enzymatically hydrolyzed animal protein and dairy products with the powdery ingredients in step (2) evenly and then carry out extrusion expansion. The temperature is about 130 °C, and the steam pressure is between 0.25 Mpa and 0.4 Mpa;

[0071] (4) Dry and dehydrate the expanded grains prepared in step (3) in segments from 120 °C to 90 °C until the moisture content ≤ 8%, and set aside;

[0072] (5) Spray chicken oil, beef tallow, and enzymatically hydrolyzed chicken liver powder on the expanded grain particles prepared in step (4);

[0073] (6) Package to obtain the finished product.

[0074] In the cat food of the present invention, small molecule proteins and fats are more easily digested and absorbed, and lactase hydrolyzes lactose in dairy products, reducing the intestinal burden. After testing, the protein digestibility is increased to 89.26%, the fat digestibility is increased to 93.21%, the addition after enzymatic hydrolysis of meat and dairy products enhances the flavor of the dry food, improves the appetite of pets, and the fecal score reaches 2.25.

[0075] Comparative Example 1

[0076] Different from Example 1, for two-stage enzymatic hydrolysis, all the enzymes are added twice instead of adding different enzymes in batches:

[0077] Adopt two-stage enzymatic hydrolysis. In the first stage, add protease at 2×10 4 U / KG, lactase at 5×10 3 U / KG, lipase at 1×10 4 U / KG, flavor protease at 2×10 4 U / KG, trypsin at 2×10 4 U / KG, at natural pH, hydrolyze at 55 °C for 120 minutes; in the second stage, at natural pH, hydrolyze at 45 °C for 90 minutes (the degree of hydrolysis reaches 35%-40%, and the proportion of molecular weight < 1000 Da exceeds 65%).

[0078] The remaining steps are the same as those in Example 1, so as to prepare a composite enzymatically hydrolyzed meat and milk mixture for preparing pet food.

[0079] Comparative Example 2

[0080] Different from Example 1, do not add lactase to enzymatically hydrolyze dairy products:

[0081] Adopt two-stage enzymatic hydrolysis. In the first stage, add protease at 2×10 4 U / KG, lipase at 1×10 4Lipase at U / KG, natural pH, enzymolysis at 55°C for 120 minutes; in the second stage, add 2×10 4 U / KG of flavor protease, 2×10 4 U / KG of trypsin, natural pH, enzymolysis at 45°C for 90 minutes (degree of hydrolysis reaches 35%-40%, proportion of molecular weight < 1000Da exceeds 65%).

[0082] Comparative Example 3

[0083] Different from Example 1, the animal protein and dairy products are not enzymolyzed and directly used as food raw materials. That is, 30 parts of chicken, 10 parts of salmon meat, and 10 parts of sheep whey powder are not enzymolyzed.

[0084] Comparative Example 4

[0085] Different from Example 1, the steps of the enzymolysis reaction are different, and only one enzymolysis is carried out and all the enzymes are added:

[0086] One-time enzymolysis is adopted, and 2×10 in terms of total amount of substance 4 U / KG of protease, 5×

[0087] 10 3 U / KG of lactase, 1×10 4 U / KG of lipase, 2×10 4 U / KG of flavor protease, 2×10 4 U / KG of trypsin, natural pH, enzymolysis at 55°C for 210 minutes.

[0088] Comparative Example 5

[0089] A certain commercially available cat food of Kaisi with added sheep milk powder.

[0090] Test 1

[0091] Detect the contents of macromolecular and small-molecular substances in the meat-milk mixtures obtained in Example 1 and Comparative Examples 1-4 (among them, Comparative Example 3 is an unenzymolyzed mixture, and the others are enzymolyzed mixtures), and the results are as follows:

[0092] For the determination of protein content, refer to the Kjeldahl method for the determination of crude protein in feeds in GB / T 6432-2018.

[0093] For the calculation of protein small molecules, the content of protein small molecules (%) = acid-soluble protein / total protein content × 100%. For the determination of acid-soluble protein content, refer to Appendix B of GB / T 22492-2008 soybean peptide powder.

[0094] For the determination of fat content, refer to the determination of crude fat in feeds in GB / T 6433-2006.

[0095] For the calculation of small fat molecules, refer to GB 5009.168-2016 National Food Safety Standard - Determination of Fatty Acids in Foods.

[0096] For the determination of lactose, refer to GB 5009.8-2023 National Food Safety Standard - Determination of Fructose, Glucose, Sucrose, Maltose and Lactose in Foods.

[0097] Table 1

[0098]

[0099] As can be seen from Table 1, the experimental results show that after enzymatic hydrolysis, the small molecules of protein and fat are significantly increased, which is beneficial to gastrointestinal absorption; and no lactose content is detected in the experiment with lactase added, which can effectively avoid lactose intolerance.

[0100] Test 2

[0101] 120 cats were divided into 6 groups, with 20 cats in each group, half male and half female, similar in body weight, and the ratio of juvenile, adult and elderly cats was approximately 3:4:3.

[0102] Animal feeding: Without pre-feeding, Group A was directly fed with the cat food of Example 1, once a day, with a feeding amount of 130 g / day per cat. They were allowed to eat and drink freely, and were fed at 9 o'clock every day. At 9 o'clock the next day, the remaining experimental food was weighed to calculate the intake. They were fed individually in cages for 20 days, and the daily food intake, fecal score, fecal quality of each cat were recorded, and the in vivo digestibility of protein and fat was measured. Groups B-F were fed with the cat foods of Comparative Examples 1-5.

[0103] Apparent protein digestibility (%) = (protein content in ingested feed - protein content in excreted feces) / protein content in ingested feed × 100%

[0104] Apparent fat digestibility (%) = (protein content in ingested feed - protein content in excreted feces) / protein content in ingested feed × 100%

[0105] The Waltham Fecal Score System (WFS) is one of the scoring systems for the sensory examination of pet feces and is also a relatively commonly used scoring system at present. Table 2 shows the WALTHAM fecal scoring criteria. Among them, when the score is 2-2.5, the feces are the healthiest, and when it exceeds 2.5, there is a tendency to have diarrhea.

[0106] Table 2

[0107] Score Fecal state 1 "Bullet-shaped", breaks with slight pressure 1.5 Hard and dry, breaks when pressure is applied 2 Formed, no mark left when picked up 2.5 Formed, slightly wet on the surface, mark left on the ground after picked up 3 Wet and soft, obvious mark left on the ground after picked up 3.5 Very wet, still has a general shape 4 Almost shapeless 4.5 Liquid state, but still has some viscosity 5 Completely liquid

[0108] Table 3

[0109]

[0110]

[0111] As can be seen from Table 3, the experimental results show that, compared with ordinary cat food, the cat food containing the compound enzymatically hydrolyzed meat and milk mixture can significantly improve the digestibility of protein and fat, and the food intake is significantly more, and can significantly improve the fecal score of cats and reduce the probability of their diarrhea.

[0112] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A composite enzymatically hydrolyzed meat and milk mixture, characterized in that, By mass parts, the composite enzymatically hydrolyzed meat and milk mixture includes: 40-60 parts of enzymatically hydrolyzed animal protein and 5-10 parts of enzymatically hydrolyzed dairy product; wherein, the enzymatically hydrolyzed animal protein includes 30-40 parts of enzymatically hydrolyzed chicken and 10-20 parts of enzymatically hydrolyzed salmon; the composite enzymatically hydrolyzed meat and milk mixture is obtained by subjecting animal protein and dairy product to two-stage enzymatic hydrolysis after adding biological enzymes.

2. The composite enzymatically hydrolyzed meat and milk mixture according to claim 1, wherein The biological enzymes include protease, lactase, lipase, flavor protease, and trypsin. Preferably, the protease is an animal protein hydrolase and the lactase is a neutral lactase. Preferably, the dairy product is goat whey powder or whole-fat goat milk powder.

3. A method for preparing the composite enzymatically hydrolyzed meat and milk mixture according to claim 1 or 2, characterized in that, It includes the following steps: (1) Raw material pretreatment: The frozen chicken and salmon meat are broken into pieces and coarsely minced into grains, and both are added to an enzymatic hydrolysis device and stirred. (2) Enzymatic hydrolysis reaction: Two-stage enzymatic hydrolysis is adopted. In the first stage, protease, lactase, and lipase are added for enzymatic hydrolysis; in the second stage, flavor protease and trypsin are added for enzymatic hydrolysis to make the degree of hydrolysis reach 35%-40%, and the proportion of molecules with a molecular weight < 1000 Da exceeds 65%.

4. The preparation method according to claim 3, characterized in that In step (1), the freezing temperature is -4°C - 0°C, the particle size of the coarsely minced grains is 3-6 mm, and the stirring time is 5-15 min.

5. The preparation method according to claim 3, characterized in that, In step (2), in the first stage, at natural pH, enzymatic hydrolysis is carried out at 50-60°C for 2-4 h, preferably 2-2.5 h; in the second stage, at natural pH, enzymatic hydrolysis is carried out at 40-50°C for 1-3 h, preferably 1-1.5 h; the enzymatic hydrolysis temperatures in the first stage and the second stage are different. Or, in step (2), in the first stage, add protease at 1×10 4 U / KG to 3×10 4 U / KG, lactase at 4×10 3 U / KG to 6×10 4 U / KG, and lipase at 0.5×10 4 U / KG to 1.5×10 4 U / KG; Add 1×10 in the second stage 4 U / KG~3×10 4 U / KG of flavor protease, 1×10 4 U / KG~3×10 4 U / KG of trypsin.

6. Use of the composite enzymatically hydrolyzed meat and milk mixture according to claim 1 or 2 or the composite enzymatically hydrolyzed meat and milk mixture obtained by the preparation method according to any one of claims 3-5 in the preparation of pet food; Preferably, the pet food is cat food.

7. A cat food for all life stages based on a composite enzymatically hydrolyzed meat and milk mixture, characterized in that, The composite enzymatically hydrolyzed meat and milk mixture according to claim 1 or 2 is adopted, and the cat food for all life stages is prepared from the following components in parts by weight: 40-60 parts of enzymatically hydrolyzed animal protein, 10-20 parts of corn, 10-20 parts of rice, 5-10 parts of enzymatically hydrolyzed dairy product, 5-10 parts of chicken oil, 2-5 parts of beef tallow, 2-3 parts of brewer's yeast powder, 1-2 parts of enzymatically hydrolyzed chicken liver powder, 1-5 parts of compound vitamins and minerals, 1-2 parts of vegetable and fruit package, 0.5-1.5 parts of algae and its products, 0.5-2 parts of other additives; wherein, the enzymatically hydrolyzed animal protein includes 30-40 parts of chicken and 10-20 parts of salmon.

8. The cat food for the whole life stage according to claim 7, characterized in that, The vegetable and fruit package includes carrot powder and cranberry powder; the mass ratio of carrot powder to cranberry powder is 15-25:

1. Preferably, the algae and its products are spirulina powder. Preferably, the other additives include antioxidant, glucosamine hydrochloride, taurine, potassium chloride, sodium chloride, and postbiotics; wherein, the mass ratio of antioxidant, glucosamine hydrochloride, taurine, potassium chloride, sodium chloride, and postbiotics is (3-5):(4-6):(120-130):(140-160):(290-310):(990-1010); The antioxidant is rosemary extract.

9. A method for preparing a cat food for all life stages based on a composite enzyme-hydrolyzed meat and milk mixture as claimed in claim 6 or 7, characterized in that, The composite enzymatically hydrolyzed meat and milk mixture described in claim 1 or 2 is adopted, and the method comprises the following steps: According to the proportion, corn and rice are crushed and then mixed evenly with brewer's yeast powder, compound vitamin and mineral, vegetable and fruit package, algae and its products, and other additives to obtain an ingredient powder. The ingredient powder and the composite enzymatically hydrolyzed meat and milk mixture are mixed and then subjected to extrusion granulation treatment. The extruded granules are subjected to gradient drying and dehydration, and then chicken fat, beef tallow and enzymatically hydrolyzed chicken liver powder are sprayed successively, and then packaged to obtain the product.

10. The preparation method according to claim 9, characterized in that, Corn and rice are crushed to pass through a 50-70 mesh sieve with 80% passing through. Preferably, the gradient drying is carried out by dehydrating in sections from 120°C to 90°C until the moisture content ≤ 8%.

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