Sports nutrition composition containing beta-alanine sustained release preparation

Abstract

The invention discloses a sports nutrition composition containing beta-alanine. The composition is prepared from the following components: beta-alanine, creatine, branched chain amino acid, coenzyme Q10 and D-ribose. In particular, the beta-alanine disclosed by the invention is a sustained-release type beta-alanine. All the components in the composition have a synergistic effect, and the composition can improve the exercise expressive force of an athlete in a short time, maintain the excellent exercise expression cycle of the athlete, delay the time of reaching a fatigue state, quickly recover the physical strength of the athlete, reduce muscular soreness and the like. In addition, an embedding material selected by the sustained-release beta-alanine does not contain allergens, is healthy and does not generate sugar, and the acceptability of consumers is higher.

Description

Technical Field

[0001] The present invention belongs to the technical field of sports nutrition supplements, and specifically relates to a sports nutrition composition containing a β-alanine sustained-release preparation. Background Art

[0002] At present, with the country's high attention to national health and strong support for industrial policies, more and more people are participating in physical exercise and fitness sprints, and the demand for sports nutrition supplements is also increasing day by day. Sports nutrition supplement products are gradually moving towards popularization. The common nutrients on the market currently mainly include protein powder, amino acids, carbohydrates, minerals, etc. There are also many sports drinks fortified with B vitamins and vitamin C, but most are limited to one or one or two combinations. There are relatively few amino acid-based sports nutrition supplement products based on Chinese population data, and most products often have a relatively single composition and cannot meet the growing product demand of sports nutrition consumers for anti-fatigue and improving sports performance.

[0003] β-alanine, also known as 3-aminopropionic acid, has the molecular formula C3H7NO2, and it is a kind of non-natural amino acid. β-alanine is a precursor substance for the synthesis of vitamin B5 and an important component of coenzyme A. According to research, β-alanine can increase the concentration of carnosine in human muscles, and carnosine can scavenge reactive oxygen species. As an effective buffer, it can prevent the production of fatigue toxins in working muscles, increase muscle strength, and delay fatigue. Therefore, β-alanine has attracted much attention in the fields of food, health care, biopharmaceuticals, and feed, and the market demand for β-alanine has also increased significantly.

[0004] With the strong promotion of β-alanine, products containing this ingredient have also emerged continuously. For example, patent CN118077889A discloses a sports endurance supplement containing β-alanine, which contains β-alanine, L-citrulline, L-tyrosine, malic acid, caffeine, taurine, and B vitamins. The combination of these raw materials can improve human endurance, relieve post-exercise fatigue, and has a synergistic effect; patent CN116391870A discloses a food nutrition formula for quickly supplementing physical energy and repairing muscle damage, including the following raw materials in parts by weight: 150 parts of glucose, 100 parts of fructose, 27 parts of collagen peptide, 19 parts of glutamine, 22 parts of sodium chloride, 11 parts of potassium citrate, 1 part of taurine, 1 part of glycine, 0.5 part of β-alanine, 0.3 part of vitamin C, and 0.01 part of vitamin B1. The said formula has the effects of significantly enhancing physical energy, reducing sports injuries, and restoring glycogen reserves in the body.

[0005] However, almost all the β-alanine raw materials used in β-alanine-containing supplement products on the market at present are non-sustained-release β-alanine. Taking these products often causes the side effect of paresthesia within 20 minutes, including but not limited to a certain degree of tingling sensation on the face, neck, back of the hand and other parts, which greatly affects the experience after taking.

[0006] Based on this, on the basis of researching sustained-release β-alanine, the present invention proposes a sports nutrition composition that meets the anti-fatigue and improves sports performance of fitness enthusiasts. Summary of the Invention

[0007] In order to solve the above technical problems, the present invention proposes a sports nutrition composition containing β-alanine, especially a sports nutrition composition containing a β-alanine sustained-release preparation (sustained-release β-alanine). The specific scheme is as follows:

[0008] In a first aspect, the present invention provides a sports nutrition composition containing β-alanine, and the composition comprises the following components: β-alanine, creatine, branched-chain amino acids, coenzyme Q10 and D-ribose.

[0009] In one or more embodiments, the composition, by weight, comprises 4-7 parts of β-alanine, 1-3 parts of creatine, 1-5 parts of branched-chain amino acids, 0.03-0.1 part of coenzyme Q10 and 1-5 parts of D-ribose.

[0010] In one or more embodiments, the β-alanine in the composition is sustained-release β-alanine, and its release rate in a simulated gastric juice pH environment is 0.5h≤55%, 1.0h≤70%, and it is completely released within 2h.

[0011] In one or more embodiments, the sustained-release β-alanine uses β-alanine as the core material and ethyl cellulose as the wall material, and the mass ratio of the core material to the wall material is 4:1-1:4; the sustained-release β-alanine is prepared by a fluidized bed coating method or a spray drying method.

[0012] As an example, the fluidized bed coating method comprises the following steps:

[0013] (1) Dilute the ethyl cellulose aqueous dispersion with water to a solid content of 10-20% as the coating solution, or configure ethyl cellulose into a 3-20% ethanol solution as the coating solution;

[0014] (2) Place β-alanine in a fluidized bed and introduce compressed air to suspend the particles;

[0015] (3) Perform coating by top spray or bottom spray process to obtain the β-alanine sustained-release preparation.

[0016] As an example, the spray drying method includes the following steps:

[0017] (1) Dissolve β-alanine in water by high-shear or heating at 50-90 °C;

[0018] (2) Prepare ethyl cellulose into an aqueous dispersion and mix it evenly with the solution formed in (1);

[0019] (3) Spray-dry the mixture obtained in (2) to obtain the β-alanine sustained-release preparation.

[0020] In one or more embodiments, the branched-chain amino acids in the composition are one or more of leucine, valine, and isoleucine.

[0021] In one or more embodiments, the branched-chain amino acids are composed of leucine, valine, and isoleucine in a mass ratio of 1:1:2 to 2:1:1.

[0022] In one or more embodiments, the composition further includes excipients acceptable in sports nutrition supplements.

[0023] In one or more embodiments, the composition is prepared into syrup, powder, granule, capsule, tablet, pill, or oral liquid.

[0024] In a second aspect, the present invention provides a preparation method of the composition described in any one of the above, and the preparation method includes the step of mixing each component evenly.

[0025] In a third aspect, the present invention further includes using the above composition to enhance sports performance in a short time, maintain an excellent sports performance cycle, delay the time to reach a fatigue state, quickly recover physical strength, and reduce muscle soreness.

[0026] Compared with the prior art, the present invention has the following advantages:

[0027] 1. The sustained-release β-alanine used in the present invention has an appropriate release rate, which can not only effectively relieve the side effects caused by taking β-alanine, but also meet the health function requirements of enhancing sports ability in a short time.

[0028] 2. The embedding material selected for the sustained-release β-alanine of the present invention does not contain allergens, is healthy and does not produce sugar, and has a higher acceptance rate among consumers.

[0029] 3. Each component in the composition of the present invention has a synergistic effect, which can enhance the sports performance of athletes in a short time, maintain the excellent sports performance cycle of athletes, delay the time to reach a fatigue state, quickly recover the physical strength of athletes, and reduce muscle soreness. Specific Embodiments

[0030] β-alanine is the simplest β-type amino acid and is a limiting amino acid for the synthesis of endogenous imidazole dipeptides in humans and mammals. It has the effects of increasing the content of imidazole dipeptides in muscles, enhancing antioxidant capacity, strengthening the buffering capacity of muscles, and anti-fatigue. Clinical studies have shown that young volunteers taking 6.4 g of β-alanine every day for 23 consecutive days have a significant increase in carnosine content. Relevant studies have also shown that supplementing β-alanine has no effect on exercises within 60 s, has a powerful positive effect on exercises between 60 - 240 s, and has a more obvious effect on exercises over 240 s. For healthy people using β-alanine supplements, the International Society of Sports Nutrition (ISSN) recommends supplementing 4 to 6 grams of β-alanine per day for at least 2 to 4 weeks, which has been proven to improve exercise performance. β-alanine can combine with histidine in skeletal muscles and other organs to form myostatin. Myostatin inhibits mitochondrial dysfunction by reducing oxidative stress caused by the accumulation of free radicals in cells, so it can reduce neuromuscular fatigue and enhance exercise performance. However, due to the excellent water solubility of β-alanine, it can be completely released within a short time in simulated gastric juice, which means that β-alanine can be rapidly absorbed by the human body, thereby causing common adverse reactions, including a strange tingling or numb feeling in the neck, face, and hands. By encapsulating and sustained-releasing β-alanine, the release of β-alanine in gastric juice can be delayed, thereby delaying the absorption of β-alanine by the human body and effectively alleviating adverse reactions such as paresthesia caused by β-alanine.

[0031] Creatine is a nitrogen-containing organic acid with the chemical formula C4H9N3O2 and the scientific name N-methylguanidinoacetic acid. It naturally exists in vertebrates and can assist in providing energy for muscle and nerve cells. Creatine is mainly stored in muscle tissues, which can reduce muscle fatigue and tension, enhance muscle elasticity, make muscles stronger, and can also accelerate the synthesis of human proteins, reduce cholesterol, blood lipids, and blood sugar, and delay the aging of the body, and play a role when the energy demand is high. By supplementing creatine, the human body can increase creatine reserves and raise the phosphocreatine level in muscles, thereby enhancing the performance of short-term, high-intensity exercises. Research has shown that creatine supplements can not only delay muscle fatigue, improve the explosive power and endurance of athletes, but also help promote muscle growth and recovery, which is an effective nutritional supplement method for fitness enthusiasts and athletes.

[0032] Branched-chain amino acids (BCAAs) include three essential amino acids for the human body, namely L-leucine, L-isoleucine, and L-valine. They cannot be synthesized in the human body and must be supplemented exogenously. BCAAs are the only amino acids that are decomposed in the liver and utilized in the muscles at the same time. As carriers of nitrogen, branched-chain amino acids assist in the synthesis of other amino acids required for muscle synthesis. Simply put, it is a process of synthesizing complex and complete muscle tissues from simple amino acids. Therefore, branched-chain amino acids stimulate the production of insulin. The main function of insulin is to allow peripheral blood glucose to be absorbed by the muscles and used as an energy source. The production of insulin also promotes the absorption of amino acids by the muscles. Branched-chain amino acids have both synthetic and anti-catabolic effects because they can significantly increase protein synthesis, promote the release of related hormones such as growth hormone (GH), IGF-1 (insulin-like growth factor-1), and insulin, and help maintain a reasonable testosterone / cortisol ratio; their anti-catabolic effect helps prevent protein breakdown and muscle loss, which is very important for those who are in the pre-exercise stage and need to control their diet. Branched-chain amino acids have a significant effect on muscle synthesis and protection. As carriers of nitrogen, they assist in the synthesis of other amino acids required for muscles, promote the synthesis of complex and complete muscle tissues from simple amino acids, thus avoiding muscle atrophy and providing energy for the muscles. In addition, branched-chain amino acids have a protective effect on muscle damage caused by exercise, can reduce muscle breakdown, and prevent excessive energy consumption. Branched-chain amino acids also play an important role in energy supply and anti-fatigue. They can provide energy through gluconeogenesis, maintain a constant blood glucose concentration, and reduce the fatigue caused by a large amount of exercise. Supplementing branched-chain amino acids can also quickly increase the blood glucose ratio, effectively inhibit the accumulation of blood lactic acid, and prevent exercise-induced fatigue.

[0033] Coenzyme Q10 (Coenzyme Q10, CoQ10), also known as ubiquinone (Ubiquinone, UQ) and coenzyme Q (Coenzyme Q, CoQ), is a coenzyme present in all eukaryotes that perform aerobic respiration. It is a benzoquinone lipid-soluble compound with a structure similar to vitamin K. Coenzyme Q10 is mainly formed in the inner mitochondrial membrane, and a small part can also be obtained through food, such as beef, eggs, fatty fish, nuts, oranges, broccoli and other fruits and vegetables. Coenzyme Q10 mainly participates in the process of mitochondrial oxidative phosphorylation and ATP production, regulates the cellular redox environment, carries reduced electrons into vesicles or out of cells during the electron transmembrane process, and participates in the formation of proton gradients across the inner and plasma membranes. It can accelerate cell renewal and stimulate cell activity, thus greatly promoting the ability of cells to uptake nutrients. For a long time, coenzyme Q10 has been the preferred nutrient for treating chronic fatigue. Clinically, it has been found that the concentration of coenzyme Q10 in patients with chronic fatigue is lower than that of the general population, and nearly 70% of the patients have improved symptoms after supplementation. In addition, in other studies, it has been found that after chronic fatigue patients supplemented with coenzyme Q10 (100 mg per day for 3 consecutive months), 90% of the patients had a significant improvement in fatigue discomfort symptoms. It can not only greatly increase exercise endurance but also make them less prone to fatigue (especially when engaging in physically demanding activities). Experiments have shown that coenzyme Q10 can greatly promote heart health, and an appropriate amount of coenzyme Q10 in the human body is necessary for proper muscle function.

[0034] D-ribose is an important pentose monosaccharide with the chemical formula C5H 10 O5. It is an important component of ribonucleic acid (RNA) and ATP and plays an important role in the formation of life. As a natural component present in all cells in the body, D-ribose is closely related to the formation of adenosine monophosphate and the regeneration of adenosine triphosphate (ATP). It is one of the most basic energy sources for life metabolism and plays a key role in the metabolism of the heart and skeletal muscles, and can promote the recovery of locally ischemic and hypoxic tissues. The direct cause of human fatigue is insufficient ATP production in muscle cells, resulting in insufficient energy for muscle activity. Supplementing D-ribose can enable athletes to restore ATP levels faster, effectively helping athletes recover from exercise fatigue and improve their ability to tolerate hypoxia during exercise. D-ribose can be synthesized in the human body, but under stressful conditions and after exercise, D-ribose levels will drop rapidly, preventing the body from replenishing energy in a timely manner. After intense exercise, taking D-ribose can increase the production of energy ATP and reduce muscle stiffness, fatigue and soreness after exercise, so it has become an important supplement for athletes.

[0035] The inventors have discovered that when β-alanine, creatine, branched-chain amino acids, coenzyme Q10, and D-ribose are combined in specific proportions, they can exhibit synergistic effects and can be used to enhance the athletic performance of athletes in a short period of time, maintain the excellent athletic performance cycle of athletes, delay the time to reach a state of fatigue, rapidly restore the physical strength of athletes, reduce muscle soreness, etc.

[0036] The composition described in the present invention may further comprise excipients acceptable in sports nutrition supplements, such as solvents, preservatives, emulsifiers, surfactants, suspending agents, wetting agents, sweeteners, solubilizers, pH buffers, etc. (such as acetates, sodium citrate, cyclodextrin derivatives, sorbitan monolaurate, triethanolamine acetate, triethanolamine oleate, and other such substances). Solvents may include glycerol, sorbitol, ethanol, and syrup; examples of preservatives may include glycerol, methylparaben, propylparaben, benzoic acid, sodium benzoate, and alcohol; examples of non-aqueous liquids for emulsions include mineral oil and cottonseed oil; examples of emulsifiers may include gelatin, gum arabic, tragacanth, bentonite, and surfactants such as polyoxyethylene sorbitan monooleate; suspending agents may include sodium carboxymethyl cellulose, pectin, tragacanth, magnesium aluminum silicate, and gum arabic; sweeteners may include sucrose, syrup, glycerol, and artificial sweeteners such as saccharin; wetting agents may include propylene glycol monostearate, sorbitan monooleate, diethylene glycol monolaurate, and polyoxyethylene lauryl ether, etc.

[0037] When preparing the composition described in the present invention, it is necessary to mix the various raw material components evenly, and then prepare different preparations according to actual needs, such as syrup, powder, granule, capsule, tablet, pill, or oral liquid and other preparations.

[0038] In one or more embodiments, the composition of the present invention can be prepared into a liquid preparation, such as an aqueous solution (such as elixirs and syrups), an emulsion, a suspension, a solution, and / or a suspension. An elixir can be a clear, sweetened hydroalcoholic preparation, and the pharmaceutically acceptable carrier for an elixir usually includes a solvent. A syrup can be a concentrated aqueous solution of sugar such as sucrose and may contain a preservative. An emulsion can be a two-phase system in which one liquid is dispersed throughout the other in the form of small globules. The pharmaceutically acceptable carrier for an emulsion can be a non-aqueous liquid, an emulsifier, and a preservative. A suspension can use suspending agents and preservatives acceptable in pharmaceuticals or health products. When prepared into a solution or suspension, water or a physiologically acceptable organic solvent such as an alcohol (such as ethanol, propanol, isopropanol, 1,2-propanediol, polyethylene glycol and its derivatives, fatty alcohols, glyceryl partial esters), oils (such as peanut oil, olive oil, sesame oil, almond oil, sunflower oil, soybean oil or castor oil), paraffin, dimethyl sulfoxide, triglycerides, etc. can be used. In the case of a liquid preparation, the following substances can be used as stabilizers or solubilizers: lower aliphatic monohydric and polyhydric alcohols containing 2-4 carbon atoms such as ethanol, n-propanol, glycerol, polyethylene glycol with a molecular weight of 200-600 (such as 1-40% aqueous solution), gum arabic, guar gum, or other suspending agents selected from hydrocolloids.

[0039] In one or more embodiments, the composition, by weight, comprises 4 to 7 parts of β-alanine, 1 to 3 parts of creatine, 1 to 5 parts of branched-chain amino acids, 0.03 to 0.1 part of coenzyme Q10, and 1 to 5 parts of D-ribose.

[0040] In one or more embodiments, the branched-chain amino acids in the composition are one or more of leucine, valine, and isoleucine.

[0041] In one or more embodiments, the branched-chain amino acids are composed of leucine, valine, and isoleucine in a mass ratio of 1:1:2 to 2:1:1.

[0042] Although the composition of the present invention can synergistically increase the exercise performance and other effects of the taker, if ordinary β-alanine is used in the composition, it will cause the side effect of paresthesia in the taker within 20 minutes after taking the product, including but not limited to a certain degree of tingling sensation in the face, neck, back of the hand and other parts, which greatly affects the experience after taking. Therefore, the inventor uses sustained-release β-histidine in the composition and finds that it hardly affects the use effect of the composition.

[0043] In the present invention, the β-alanine sustained-release preparation or the sustained-release β-alanine have the same meaning, and the "sustained release" therein can be regarded as being substantially equivalent to "continuous release": continuous release, controlled release, delayed release, reservoir, gradual release, long-term release, programmed release, extended release, proportional release, retarded release, storage, slowness, slow release, intermittent release, timed coating, timed release, delayed action, extended action, time-sharing action, long-acting, prolonged effect, repeated action, slow action, continuous action, continuous action administration, and extended release. For more discussions on these terms, reference can be made to Lesczek Krowczynski, Extended-Release Dosage Forms, 1987 (CRC Press, Inc.).

[0044] Specifically, the sustained-release β-histidine of the present invention uses β-alanine as the core material and ethyl cellulose as the wall material, and the mass ratio of the core material to the wall material is 4:1 to 1:4; the sustained-release β-alanine is prepared by a fluidized bed coating method or a spray drying method.

[0045] Ethyl cellulose as a coating material for sustained-release preparations is already common knowledge in the art. However, under normal circumstances, the microcapsules coated with ethyl cellulose wall materials have poor sustained-release effects. Although they can tolerate gastric acid and reach the intestine, it is difficult for the active substances to be released in the stomach, and they cannot be stably or completely released in the intestine, resulting in part of them being excreted out of the body, reducing the utilization rate of the pharmaceutical preparation. However, the inventors found that using ethyl cellulose as the wall material for β-alanine sustained-release preparations can effectively release β-alanine at a reasonable rate.

[0046] As an example, the fluidized bed coating method includes the following steps:

[0047] (1) Dilute the ethyl cellulose aqueous dispersion with water to a solid content of 10-20% as the coating solution, or prepare an ethanol solution of ethyl cellulose at 3-20% as the coating solution;

[0048] (2) Place β-alanine in the fluidized bed and introduce compressed air to suspend the particles;

[0049] (3) Perform coating using a top spray or bottom spray process to obtain the β-alanine sustained-release preparation.

[0050] The present invention does not limit the specific fluidized bed coating conditions, which can be adjusted according to actual needs. As a preferred fluidized bed coating condition: introduce compressed air into β-alanine to suspend the particles, the air intake is 1400 R / min, and the inlet air temperature is controlled at 60 °C; for top spray coating: the atomization pressure is 0.1 MPa, the coating temperature (in the middle cavity) is 48-52 °C, and the coating solution flow rate is 2-4 R / min.

[0051] As an example, the spray drying method includes the following steps:

[0052] (1) Dissolve β-alanine in water by means of high shear or heating at 50-90 °C;

[0053] (2) Prepare ethylcellulose into an aqueous dispersion and mix it evenly with the solution formed in (1);

[0054] (3) Spray dry the mixture obtained in (2) to obtain the β-alanine sustained-release preparation.

[0055] The sustained-release β-alanine prepared according to the above method has a release rate of ≤55% at 0.5 h, ≤70% at 1.0 h, and is completely released within 2 h under the simulated gastric juice pH environment.

[0056] It should be understood that the sustained-release β-alanine described in the present invention contains β-alanine and ethylcellulose material. Therefore, when adding this sustained-release β-alanine to the composition described in the present invention, the weight ratio of β-alanine in the composition should be calculated based on the content of β-alanine in the actually prepared sustained-release β-alanine. For example, when the sustained-release β-alanine is prepared from β-alanine and ethylcellulose in a mass ratio of 1:1, when adding this sustained-release β-alanine to the composition described in the present invention, the addition amount of this sustained-release β-alanine should be added according to twice the addition amount of β-alanine.

[0057] Example

[0058] The following specific examples illustrate the implementation manners of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation manners, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0059] When the embodiments give a numerical range, it should be understood that unless otherwise specified in the present invention, any value between the two endpoints of each numerical range and either endpoint can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0060] For those technical or conditions not specified in the examples, they shall be carried out according to the techniques or conditions described in the literature in this field or according to the product specifications. For the reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through regular channels.

[0061] In the examples, the addition amounts, contents, and concentrations of various substances are involved. Unless otherwise specified, the percentage content refers to the mass percentage content.

[0062] The sources of the materials in the following examples and comparative examples are as follows:

[0063] β-alanine was purchased from Zhejiang Huarui Biotechnology Co., Ltd.;

[0064] Ethyl cellulose was purchased from Tianjin Aileyi Pharmaceutical Materials Co., Ltd.;

[0065] Creatine was purchased from Jiangsu Weizhirun Biotechnology Co., Ltd.;

[0066] Branched-chain amino acids were purchased from Luoyang Huarong Biotechnology Co., Ltd.;

[0067] Coenzyme Q10 was purchased from Zhejiang Yicun Biotechnology Co., Ltd.;

[0068] D-ribose was purchased from Shandong Huiheng Biotechnology Co., Ltd.

[0069] The detection method for the release rate of sustained-release β-alanine in the simulated gastric juice pH environment in the following examples:

[0070] Calculated according to the daily gastric juice secretion volume of 1 L for adults and the β-alanine intake of 3 g, add the preparation sample (containing 0.15 g of β-alanine) to 50 ml of 0.1 mol / L hydrochloric acid solution and place it in a 37°C constant temperature water bath. At regular times (3 min, 30 min, 60 min, and 120 min), pipette 1 ml of the clear liquid to measure the effective content of β-alanine in the solution, and calculate the release percentage.

[0071] The determination method for β-alanine content: Refer to the literature "Determination of β-alanine content in conversion solution by high performance liquid chromatography, Liu Licong et al., Industrial Technology Innovation. 2017, 04(03)".

[0072] Release percentage = β-alanine content in the clear liquid / 0.003 * 100%, where 0.003 is the theoretically achievable concentration when β-alanine is completely released.

[0073] Example 1:

[0074] A sports nutrition composition containing β-alanine, which comprises the following components: 4 g of β-alanine, 1 g of creatine, 3 g of leucine, 0.05 g of coenzyme Q10, and 3 g of D-ribose.

[0075] The preparation method of this composition is: weigh each component by weight, and then mix them evenly.

[0076] Example 2:

[0077] A sports nutrition composition containing β-alanine, which comprises the following components: 5 g of β-alanine, 1 g of creatine, 1 g of leucine, 1 g of isoleucine, 0.03 g of coenzyme Q10, and 1 g of D-ribose.

[0078] The preparation method of the composition is: weighing each component by weight, and then mixing evenly.

[0079] Example 3:

[0080] A sports nutrition composition containing β-alanine, which comprises the following components: 4 g of β-alanine, 2 g of creatine, 2 g of leucine, 1 g of valine, 0.05 g of coenzyme Q10, and 2 g of D-ribose.

[0081] The preparation method of the composition is: weighing each component by weight, and then mixing evenly.

[0082] Example 4:

[0083] A sports nutrition composition containing β-alanine, which comprises the following components: 5 g of β-alanine, 2 g of creatine, 1 g of leucine, 1 g of valine, 2 g of isoleucine, 0.1 g of coenzyme Q10, and 3 g of D-ribose.

[0084] The preparation method of the composition is: weighing each component by weight, and then mixing evenly.

[0085] Example 5:

[0086] A sports nutrition composition containing β-alanine, which comprises the following components: 7 g of β-alanine, 3 g of creatine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, 0.1 g of coenzyme Q10, and 1 g of D-ribose.

[0087] The preparation method of the composition is: weighing each component by weight, and then mixing evenly.

[0088] Example 6:

[0089] A sports nutrition composition containing β-alanine, which comprises the following components: 6 g of β-alanine, 2 g of creatine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, 0.05 g of coenzyme Q10, and 5 g of D-ribose.

[0090] The preparation method of the composition is: weighing each component by weight, and then mixing evenly.

[0091] Example 7:

[0092] A sports nutrition composition containing β-alanine, which comprises the following components: 6 g of β-alanine, 2 g of creatine, 2.5 g of leucine, 1.5 g of valine, 1 g of isoleucine, 0.05 g of coenzyme Q10, and 4 g of D-ribose.

[0093] The preparation method of the composition is as follows: Weigh each component by weight and then mix them evenly.

[0094] Example 8:

[0095] A sports nutrition composition containing β-alanine, which comprises the following components: 12 g of sustained-release β-alanine, 2 g of creatine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, 0.05 g of coenzyme Q10, and 5 g of D-ribose.

[0096] The preparation method of the composition is as follows: Weigh each component by weight and then mix them evenly.

[0097] Among them, the sustained-release β-alanine is prepared by the fluidized bed coating method, in which the mass ratio of the core material β-alanine to the wall material ethyl cellulose is 1:1. The specific preparation steps are as follows:

[0098] Shake the ethyl cellulose aqueous dispersion (25% solid content) well before use, and then quantitatively weigh 10 g of the ethyl cellulose aqueous dispersion and dilute it with pure water to a solid content of 15% for standby. Weigh 10 g of β-alanine, introduce compressed air to suspend the particles, with an air inlet flow rate of 1400 R / min, and control the inlet air temperature at 60 °C. Top spray coating: The atomization pressure is 0.1 MPa, the coating temperature (in the middle cavity) is 48 °C, and the coating liquid flow rate is 3 R / min. The release rate of the obtained sustained-release β-alanine in the simulated gastric juice pH environment is 54.38% at 30 min, 68.35% at 60 min, and it is completely released at 120 min.

[0099] Example 9:

[0100] A sports nutrition composition containing β-alanine, which comprises the following components: 7.5 g of sustained-release β-alanine, 2 g of creatine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, 0.05 g of coenzyme Q10, and 5 g of D-ribose.

[0101] The preparation method of the composition is as follows: Weigh each component by weight and then mix them evenly.

[0102] Among them, the sustained-release β-alanine is prepared by the spray drying method, in which the mass ratio of the core material β-alanine to the wall material ethyl cellulose is 4:1. The specific preparation steps are as follows:

[0103] Weigh 20 g of β-alanine, add 27 g of water, heat up to 70 °C until completely dissolved, then add 20 g of ethyl cellulose aqueous dispersion (ethyl cellulose content is 25%), stir evenly, and set aside. The spray drying conditions are as follows: inlet air temperature 180 °C, outlet air temperature 90 °C, inlet air volume 50%, liquid feeding speed 7%, inlet air speed 600 L / h, air pressure 0.3 Mpa. The release rate of the obtained sustained-release β-alanine is 53.67% at 30 min, 67.24% at 60 min, and is completely released at 120 min under the pH environment of simulated gastric juice.

[0104] Example 10:

[0105] A sports nutrition composition containing β-alanine, which comprises the following components: 30 g of sustained-release β-alanine, 2 g of creatine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, 0.05 g of coenzyme Q10, and 5 g of D-ribose.

[0106] The preparation method of this composition is as follows: Weigh each component by weight and then mix evenly.

[0107] Among them, the preparation of the sustained-release β-alanine adopts the spray drying method, in which the mass ratio of the core material β-alanine to the wall material ethyl cellulose is 1:4. The specific preparation steps are as follows:

[0108] Shake the ethyl cellulose aqueous dispersion (25% solid content) well before use, then quantitatively weigh 80 g of ethyl cellulose aqueous dispersion and dilute it with pure water to 15% solid content, and set aside. Weigh 5 g of β-alanine, introduce compressed air to suspend the particles, the inlet air volume is 1400 R / min, and the inlet air temperature is controlled at 60 °C. Top spray coating: atomization pressure 0.1 MPa, coating temperature (middle cavity) 52 °C, coating liquid flow rate 2 R / min. The release rate of the obtained β-alanine preparation product is 45.13% at 30 min, 56.85% at 60 min, and is completely released at 120 min under the pH environment of simulated gastric juice.

[0109] Comparative Example 1:

[0110] A sports nutrition composition containing β-alanine, which comprises the following components: 6 g of β-alanine, 2 g of creatine, 2 g of leucine, 1 g of valine, and 1 g of isoleucine.

[0111] Comparative Example 2:

[0112] A sports nutrition composition containing β-alanine, which comprises the following components: 6 g of β-alanine, 2 g of creatine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, and 0.05 g of coenzyme Q10.

[0113] Comparative Example 3:

[0114] A sports nutrition composition containing β-alanine, which comprises the following components: 6 g of β-alanine, 2 g of creatine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, and 4 g of D-ribose.

[0115] Comparative Example 4:

[0116] A sports nutrition composition containing β-alanine, which comprises the following components: 2 g of creatine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, 0.05 g of coenzyme Q10, and 4 g of D-ribose.

[0117] Comparative Example 5:

[0118] A sports nutrition composition containing β-alanine, which comprises the following components: 6 g of β-alanine, 2 g of creatine, 0.05 g of coenzyme Q10, and 4 g of D-ribose.

[0119] Comparative Example 6:

[0120] A sports nutrition composition containing β-alanine, which comprises the following components: 6 g of β-alanine, 2 g of leucine, 1 g of valine, 1 g of isoleucine, 0.05 g of coenzyme Q10, and 4 g of D-ribose.

[0121] Detection Example 1: Verify whether it can improve sports performance through the grip strength of mice

[0122] (1) Mouse preparation:

[0123] Select male C57BL / 6 mice that are fully grown and have mature bones, that is, 5-month-old male C57BL / 6 mice (5-month-old mice have experienced the rapid bone growth stage during development). Under standard conditions, there are four to five mice in each cage for 1 week. During this period, the mice are fed a normal diet (2018 Teklad Global 18% protein extruded rodent diet, ENVIGO) and water, and kept at a constant temperature of 25°C and a 12-hour light / dark schedule. Then, mice weighing 30 ± 1 g are placed in separate single cages and used for the experiment.

[0124] (2) Drug preparation:

[0125] Dissolve the compositions of Examples 1-7 and Comparative Examples 1-6 in pure water respectively to prepare a solution of 30 mg / mL, and intervene at a dose of 100 mg / kg / day according to the mouse body weight. Each mouse is intraperitoneally injected with a volume of 100 μL.

[0126] (3) Animal grouping:

[0127] Seven days before the start of the experiment, the mice were randomly divided into groups a - n, with 5 mice in each group. In addition to normal feeding, groups a - m (where groups a - g corresponded to Examples 1 - 7 and groups h - m corresponded to Comparative Examples 1 - 6) were intraperitoneally injected with 100 μl of the corresponding drug every day, and group n, as the control group, was injected with 100 μl of pure water.

[0128] (4) Instrument: Shanghai Xinruan XR501 type grip strength meter for mice and rats (handgrip dynamometer).

[0129] (5) Steps of the grip strength experiment:

[0130] Before the test, weigh each mouse, and bring the test mice into the test room at least one hour before the test to adapt to the environment. Gently take out the mouse from the test cage, gently hold its tail, and allow the animal to grasp the digital dynamometer with all four paws while being gently pulled parallel to the rod by the tail. When applying the maximum force to the experimental mouse (the speed should be slow enough so that at least one paw of the mouse leaves the dynamometer), record the reading on the grip strength meter, in Newtons (N). Repeat this process to obtain 3 measurements of the four - limb grip strength and calculate the average value, which is the grip strength of each mouse. For each group of 5 mice, add up the grip strengths of each mouse and calculate the average value, which is the grip strength of the corresponding group of mice.

[0131] (6) The experimental results are shown in Table 1:

[0132] Table 1 Experimental results of the grip strength of mice

[0133] Group Grip strength (N) a 2.2 b 2.3 c 2.3 d 2.2 e 2.4 f 2.5 g 2.4 h 2.0 i 2.0 j 1.8 k 1.9 l 2.0 m 1.8 n 1.6

[0134] From the above data, it can be seen that compared with the blank control group n, the grip strength of the mice in groups h - n corresponding to Comparative Examples 1 - 6 has been significantly improved, with the highest increase of 25%; compared with groups h - n, the grip strength of the mice in groups a - g corresponding to Examples 1 - 7 has been further improved, with the highest increase of 38%. Therefore, the components of the composition described in the present invention have a synergistic effect and can be used to improve the sports performance of athletes in a short period of time.

[0135] Detection Example 2: Verify whether it can maintain the excellent sports performance cycle of athletes through the mouse running wheel experiment

[0136] Prepare the mice and drugs according to steps (1) - (3) in Detection Example 1, and conduct the grouping of the mice. The method of the running wheel experiment is as follows:

[0137] Before each experiment, mice in groups a - m were intraperitoneally injected with 100 μl of the corresponding composition solution, and mice in group n were injected with 100 μl of pure water as a control group. After 1 h, the mice were placed in individual cages equipped with a locked 14 - cm - diameter running wheel and a rotation counter, and the total number of laps they ran in the running wheel within 1 h was recorded, as well as their physical status after acute exercise. This experiment was carried out continuously for multiple days with the same experimental time period each day until the exercise performance of all mice significantly declined (the number of laps run within 1 h decreased by 20% compared to the first day), that is, until they reached a state of exhaustion. There were 5 mice in each group. The number of days it took for each mouse to reach a state of exhaustion was added up and the average was calculated, which was the number of days it took for each group to reach a state of exhaustion.

[0138] We observed that on the first day of the experiment, each mouse quickly adapted to the running wheel and ran in the running wheel quite cheerfully, although the running frequency and physical strength significantly declined in the later stage of the 1 - h period. In the following days, the exercise performance of each group of mice was quite similar. First, it maintained a situation similar to that on the first day, and then reached a critical point on a certain day, after which the exercise performance significantly declined. The statistical results of the number of days it took for each group of mice to reach a state of exhaustion are shown in Table 2.

[0139] Table 2 Results of the mouse running wheel experiment

[0140] Group Number of days to reach exhaustion (d) a 15.2 b 15.6 c 16.1 d 15.4 e 16.5 f 16.2 g 15.8 h 13.4 i 13.2 j 12.9 k 13.1 l 13.5 m 13.4 n 10.3

[0141] From the above data, it can be seen that compared with the blank control group n, the time for mice in groups h - n corresponding to Comparative Examples 1 - 6 to reach a state of exhaustion was significantly delayed, with a maximum delay of 31%; compared with groups h - n, the time for mice in groups a - g corresponding to Examples 1 - 7 to reach a state of exhaustion was further delayed, with a maximum delay of 28%. Therefore, the components of the composition described in the present invention have a synergistic effect, can delay the time to reach a state of fatigue, and can be used to maintain the excellent exercise performance cycle of athletes.

[0142] Detection Example 3: Verify whether physical strength can be quickly restored by combining the mouse running wheel experiment and grip strength verification

[0143] Prepare mice and drugs according to steps (1) - (3) in Detection Example 1, and group the mice.

[0144] Each mouse in each group was placed in an individual cage equipped with a locked 14 - cm - diameter running wheel and a rotation counter, and allowed to run freely for 1 h. Then, mice in groups a - m were intraperitoneally injected with 100 μl of the corresponding composition solution, and mice in group n were injected with 100 μl of pure water as a control group. Half an hour later, a grip strength experiment was carried out according to step (5) in Detection Example 1. The ratio of the grip strength result of each group of mice after running the running wheel to the grip strength result without running the running wheel was used as an index of physical strength recovery to verify whether the composition can quickly restore physical strength.

[0145] The grip strength result data is shown in Table 3, where the grip strength without running wheel is the data in Table 1.

[0146] Table 3 Experimental results of grip strength of mice after running wheel first

[0147]

[0148] From the above data, it can be seen that the physical strength recovery of the blank control group n is limited; the physical strength recovery of the h-n group of mice corresponding to Comparative Examples 1-6 can reach up to 83.3%; the physical strength recovery of the a-g group of mice corresponding to Examples 1-7 is better, and the physical strength recovery can reach up to 92.0%. Therefore, the components of the composition described in the present invention have a synergistic effect and can be used to quickly recover the physical strength of athletes.

[0149] Detection Example 4: Verify the physical feeling and muscle soreness after exercise of taking the composition through running enthusiasts

[0150] A total of 40 running enthusiasts (all informed consent volunteers) with similar height, weight, running duration, and running speed were recruited and randomly divided into four groups, A, B, C, and D, with 10 people in each group.

[0151] Taking the compositions in Examples 6, 8, 9, and 10 as oral agents for the running enthusiasts in groups A, B, C, and D. The components in the four compositions are basically the same, except that the β-alanine in Example 6 is a common product, and the β-alanine in Examples 8-10 is a sustained-release β-alanine prepared by the method described in the present invention.

[0152] According to the compositions in each example, take one-third of the dose and dissolve it in an appropriate amount of water, and the running enthusiasts in each group take it orally. After resting for a while, they set off together for a long-distance run. Record the physical feeling of each group at 10 min, 20 min, 30 min, and 60 min during running, and record the muscle soreness of the running enthusiasts in each group at 60 min. The specific results are shown in Table 4.

[0153] Table 4 Conditions after running enthusiasts take it

[0154]

[0155]

[0156] From the above data, it can be seen that whether the present invention uses sustained-release β-alanine or common β-alanine, the muscle soreness of running enthusiasts after running for 60 min has been significantly reduced. In addition, compared with using common β-alanine, using the sustained-release β-alanine prepared by the present invention does not produce side effects such as tingling caused by common β-alanine.

[0157] In addition, it should be understood that although this specification is described in terms of embodiments, not every embodiment contains only an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A sports nutrition composition containing β-alanine, characterized in that The composition comprises the following components: beta-alanine, creatine, branched-chain amino acids, coenzyme Q10, and D-ribose.

2. The composition according to claim 1, characterized in that, The composition, by weight parts, comprises 4-7 parts of beta-alanine, 1-3 parts of creatine, 1-5 parts of branched-chain amino acids, 0.03-0.1 part of coenzyme Q10, and 1-5 parts of D-ribose.

3. The composition according to claim 1, wherein The beta-alanine in the composition is sustained-release beta-alanine, and its release degrees under the pH environment of simulated gastric juice are as follows: within 0.5 h ≤ 55%, within 1.0 h ≤ 70%, and completely released within 2 h.

4. The composition according to claim 3, characterized in that, The sustained-release beta-alanine uses beta-alanine as the core material and ethyl cellulose as the wall material, and the mass ratio of the core material to the wall material is 4:1 to 1:4; the sustained-release beta-alanine is prepared by a fluidized bed coating method or a spray drying method.

5. The composition according to claim 1, wherein The branched-chain amino acids in the composition are one or more of leucine, valine, and isoleucine.

6. The composition according to claim 5, wherein The branched-chain amino acids are composed of leucine, valine, and isoleucine according to the mass ratio of 1:1:2 to 2:1:

1.

7. The composition according to claim 1, wherein The composition further includes excipients acceptable in sports nutrition supplements.

8. The composition according to claim 1, wherein The composition is prepared into syrup, powder, granule, capsule, tablet, pill, or oral liquid.

9. A method for preparing the composition according to any one of claims 1-8, characterized in that, The preparation method includes the step of uniformly mixing each component.

10. The composition according to any one of claims 1-8 is used for enhancing exercise performance in a short time, maintaining an excellent exercise performance cycle, delaying the time to reach the fatigue state, quickly recovering physical strength, and reducing muscle soreness.

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