Blue copper peptide composition with anti-wrinkle repairing function and preparation method thereof

Abstract

The invention discloses a blue copper peptide composition with anti-wrinkle and repairing functions and a preparation method of the blue copper peptide composition. The blue copper peptide composition comprises a solution A and a solution B, the solution A comprises a blue copper peptide microcapsule, a humectant, a thickening agent, an antioxidant and water, and the blue copper peptide microcapsule comprises blue copper peptide serving as a core and a polydopamine layer wrapping the blue copper peptide; the solution B comprises a polydopamine decomposing agent and water, the polydopamine decomposing agent is used for decomposing a polydopamine layer wrapping the blue copper peptide, and the polydopamine decomposing agent does not decompose the blue copper peptide. Before the blue copper peptide composition with the anti-wrinkle repairing function is used, the solution B and the solution A need to be mixed, and the dopamine decomposing agent decomposes the polydopamine layer wrapping the blue copper peptide, so that after the solution B and the solution A are mixed, the polydopamine layer is damaged, the blue copper peptide is released, and the anti-wrinkle repairing function is achieved. And peptide hydrolysis and premature release of copper ions caused by premature dissolution of the copper blue peptide in a liquid phase are avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a blue copper peptide composition with anti-wrinkle and repairing functions and a preparation method thereof. Background Art

[0002] Blue copper peptide (tripeptide-1 copper, GHK-Cu) is a complex formed by glycyl-histidyl-lysine (Gly-His-Lys, GHK) and copper ions (Cu2+). Blue copper peptide is a bioactive peptide with anti-aging, anti-wrinkle, repair, anti-oxidation, skin regeneration, wound healing and tissue repair functions. It is widely used in skin care products, medicine and biochemistry.

[0003] Blue copper peptides are generally prepared by mixing GHK and copper salts in liquid phase. Blue copper peptides need to function in the form of a complex of GHK and copper ions, because there are many important enzymes in the human body and on the skin that require Cu ions. These enzymes play a role in connective tissue formation, antioxidant defense, and cell respiration. Cu ions also play a signaling function and can affect cell behavior and metabolism.

[0004] The pH value of healthy oriental skin is between 4.5 and 6.5, which is slightly acidic. This acidic environment helps maintain the skin barrier function and inhibits the growth of harmful microorganisms. Therefore, the pH value of skin care products should be as close to this range as possible to ensure its mildness and effectiveness. Although occasional use of strong acid or strong alkaline skin care products will not have much impact on the skin, long-term use of such skin care products is likely to affect the skin, causing skin sensitivity and even accelerating skin aging. Generally speaking, according to product type, the ideal pH value of cleansing products is 5.0-7.0, the ideal pH value of toner / lotion is 4.5-5.5, the ideal pH value of vitamin C essence is 3.0-3.5, the ideal pH value of fruit acid essence is 3.0-4.0, the ideal pH value of peptide or ordinary essence is 5.0-6.0, the ideal pH value of cream / lotion is 5.0-6.0, the ideal pH value of fruit acid exfoliating products is pH3.0-4.0, the ideal pH value of enzyme exfoliating products is pH 5.0-7.0, and the ideal pH value of sunscreen products is 5.0-6.0. It can be seen from this that the pH value of a large number of skin care products is lower than 5, and the lowest can even be as low as 3.

[0005] However, blue copper peptide is most stable in an environment with a pH of 5.0 to 7.0. This pH range can minimize the hydrolysis of the peptide and the premature release of copper ions while maintaining the biological activity of blue copper peptide. Blue copper peptide in an environment with a pH below 5 will lead to accelerated hydrolysis of peptide bonds, destroying the structure of blue copper peptide, and will also lead to premature release of copper ions, reducing the bioavailability of blue copper peptide.

[0006] That is to say, during the storage process of the composition containing copper tripeptide, problems such as hydrolysis of the peptide in the copper tripeptide and premature release of copper ions will occur, and the problems become more and more serious as the storage time of the composition containing copper tripeptide prolongs. Summary of the Invention

[0007] Based on this, it is necessary to provide a copper tripeptide composition with anti-wrinkle and repair functions that can solve the above problems.

[0008] In addition, it is also necessary to provide a preparation method using the above copper tripeptide composition with anti-wrinkle and repair functions.

[0009] A copper tripeptide composition with anti-wrinkle and repair functions, comprising: liquid A and liquid B;

[0010] The liquid A includes copper tripeptide microcapsules, a humectant, a thickener, an antioxidant, and water. The copper tripeptide microcapsules include copper tripeptide as the core and a polydopamine layer wrapped outside the copper tripeptide;

[0011] The liquid B includes a polydopamine decomposer and water. The polydopamine decomposer is used to decompose the polydopamine layer wrapped outside the copper tripeptide, and the polydopamine decomposer does not decompose the copper tripeptide.

[0012] In one embodiment, the polydopamine decomposer is peroxidase.

[0013] In one embodiment, the polydopamine decomposer is lactoperoxidase.

[0014] In one embodiment, by mass, the liquid A includes 0.5 parts to 2.5 parts of the copper tripeptide microcapsules, 2 parts to 8 parts of the humectant, 1.5 parts to 2.5 parts of the thickener, 1 part to 3 parts of the antioxidant, and 80 parts to 120 parts of water. The mass ratio of the polydopamine decomposer to the copper tripeptide microcapsules is 0.01 - 0.1:0.5 - 2.5.

[0015] In one embodiment, the thickness of the polydopamine layer is 1 nm to 50 nm.

[0016] In one embodiment, the humectant is selected from at least one of glycerol and glucosylglycerol.

[0017] In one embodiment, the thickener is selected from at least one of sodium carboxymethyl cellulose, hydroxyethyl cellulose, and sodium alginate.

[0018] In one embodiment, the antioxidant is selected from at least one of cysteine and ascorbic acid.

[0019] A preparation method of a copper tripeptide composition with anti-wrinkle and repair functions as described in any one of the above embodiments, comprising the following steps:

[0020] Prepare an aqueous solution of a thickener, disperse a humectant and an antioxidant in water, then add the aqueous solution of the thickener, and after stirring evenly, add copper tripeptide microcapsules to obtain solution A, wherein the copper tripeptide microcapsules include copper tripeptide as the core and a polydopamine layer wrapped outside the copper tripeptide;

[0021] Dissolve a polydopamine decomposer in water to obtain solution B, wherein the polydopamine decomposer is used to decompose the polydopamine layer wrapped outside the copper tripeptide, and the polydopamine decomposer does not decompose the copper tripeptide.

[0022] In one embodiment, the copper tripeptide microcapsules are prepared by the following operations:

[0023] Add a dopamine hydrochloride solution with a pH of 8-9 and a concentration of 1 mg / mL-5 mg / mL to a copper tripeptide solution, stir and react for 12 h-24 h to cause the dopamine to undergo a self-polymerization reaction, forming the polydopamine layer wrapped outside the copper tripeptide to obtain the copper tripeptide microcapsules.

[0024] When the copper tripeptide composition with anti-wrinkle and repair functions of the present invention is stored, the copper tripeptide can be protected by wrapping the copper tripeptide with a polydopamine layer, avoiding the hydrolysis of the peptide and the premature release of copper ions caused by the dissolution of the copper tripeptide under acidic conditions, and improving the bioavailability of the copper tripeptide.

[0025] When the copper tripeptide composition with anti-wrinkle and repair functions of the present invention is used, mix solution B including a polydopamine decomposer with solution A including copper tripeptide, and the polydopamine decomposer can decompose the polydopamine layer wrapped outside the copper tripeptide, and the polydopamine decomposer does not decompose the copper tripeptide, so that after mixing solution B and solution A, the polydopamine layer can be destroyed and the copper tripeptide can be released, avoiding the hydrolysis of the peptide and the premature release of copper ions caused by the premature dissolution of the copper tripeptide in the liquid phase.

[0026] Preferably, the polydopamine decomposer is peroxidase. Polydopamine contains a large number of catechol (polyphenol) structures, which are easily recognized and catalytically oxidized by peroxidase. After the polyphenol structure is catalytically oxidized, the molecular structure of polydopamine may be broken or recombined, so that the polydopamine film ruptures, and then the internal copper tripeptide is released. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required for the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can be obtained based on these drawings.

[0028] Wherein:

[0029] Figure 1 It is a flowchart of a preparation method of a blue copper peptide composition with anti-wrinkle and repair functions in an embodiment. Specific embodiments

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0031] The present invention discloses a blue copper peptide composition with anti-wrinkle and repair functions in an embodiment, including: liquid A and liquid B.

[0032] Liquid A includes blue copper peptide microcapsules, a moisturizer, a thickener, an antioxidant, and water. The blue copper peptide microcapsules include blue copper peptides as the core and a polydopamine layer wrapped around the blue copper peptides.

[0033] Blue copper peptide is a bioactive peptide with functions such as anti-aging, anti-wrinkle, repair, antioxidant, promoting skin regeneration, promoting wound healing, and tissue repair.

[0034] The polydopamine layer wrapped around the blue copper peptides can protect the blue copper peptides, avoiding the hydrolysis of the peptides and the premature release of copper ions caused by the dissolution of the blue copper peptides under acidic conditions, and improving the bioavailability of the blue copper peptides.

[0035] Liquid B includes a polydopamine decomposer and water. The polydopamine decomposer is used to decompose the polydopamine layer wrapped around the blue copper peptides, and the polydopamine decomposer does not decompose the blue copper peptides.

[0036] The polydopamine decomposer does not decompose the blue copper peptides, thus avoiding the decomposition of the blue copper peptides by the polydopamine decomposer after the mixture of liquid B and liquid A.

[0037] When the copper - peptide - blue composition with anti - wrinkle and repair functions of the present invention is stored, the copper - peptide - blue is protected by wrapping it with a polydopamine layer, which avoids the hydrolysis of the peptide and the premature release of copper ions caused by the dissolution of the copper - peptide - blue under acidic conditions, and improves the bioavailability of the copper - peptide - blue.

[0038] When the copper - peptide - blue composition with anti - wrinkle and repair functions of the present invention is used, liquid B containing a polydopamine decomposer needs to be mixed with liquid A containing the copper - peptide - blue. The polydopamine decomposer is used to decompose the polydopamine layer wrapped outside the copper - peptide - blue, and the polydopamine decomposer does not decompose the copper - peptide - blue. Thus, after liquid B and liquid A are mixed, the polydopamine layer can be destroyed, and the copper - peptide - blue can be released, avoiding the hydrolysis of the peptide and the premature release of copper ions caused by the premature dissolution of the copper - peptide - blue in the liquid phase.

[0039] It should be noted that before the copper - peptide - blue composition with anti - wrinkle and repair functions of the present invention is used, liquid B containing a polydopamine decomposer needs to be mixed with liquid A containing the copper - peptide - blue. Therefore, in product design, liquid A can be stored in a large container, and liquid B can be filled in a small container and then stored in the large container. When the product is opened, the small container is opened simultaneously, so that liquid A and liquid B are mixed. After mixing, it needs to be shaken and placed for 24 h - 48 h before use.

[0040] Preferably, in this embodiment, the polydopamine decomposer is peroxidase. It should be noted that polydopamine can also be decomposed by enzymes such as protease and lysozyme, but since these enzymes will also decompose the copper - peptide - blue at the same time, these enzymes are not selected in the present invention.

[0041] Polydopamine contains a large number of catechol (polyphenol) structures, which are easily recognized and catalytically oxidized by peroxidase. After the polyphenol structure is catalytically oxidized, the molecular structure of polydopamine breaks or recombines, so that the polydopamine film ruptures, and then the internal copper - peptide - blue is released.

[0042] There is no catechol (polyphenol) - like structure in the copper - peptide - blue, so catalase usually does not react with the copper - peptide.

[0043] More preferably, in this embodiment, the peroxidase is lactoperoxidase.

[0044] Lactoperoxidase (LPO) is an enzyme naturally present in mammalian milk, saliva, and tears. Lactoperoxidase extracted from natural sources (such as cow's milk or plants) has powerful antibacterial, antioxidant, and anti-inflammatory properties. Lactoperoxidase can scavenge free radicals, reduce the damage of oxidative stress to the skin, thus having an antioxidant effect, helping to delay skin aging, improve wrinkles and fine lines. Lactoperoxidase is also suitable for sensitive skin or inflammatory skin problems (such as eczema, rosacea), meeting consumers' demand for natural ingredients.

[0045] Polydopamine contains a large number of catechol (polyphenol) structures, which are easily recognized and catalytically oxidized by lactoperoxidase. After the polyphenol structure is catalytically oxidized, the molecular structure of polydopamine breaks or recombines, resulting in the rupture of the polydopamine film and the release of the internal copper tripeptide.

[0046] Preferably, in this embodiment, by mass fraction, the liquid A includes 0.5 to 2.5 parts of copper tripeptide microcapsules, 2 to 8 parts of humectant, 1.5 to 2.5 parts of thickener, 1 to 3 parts of antioxidant, and 80 to 120 parts of water. The mass ratio of the polydopamine decomposer to the copper tripeptide microcapsules is 0.01 - 0.1:0.5 - 2.5.

[0047] Among them, the volume ratio of the liquid A and the liquid B can be set according to the actual situation, as long as the mass ratio of the polydopamine decomposer to the copper tripeptide microcapsules is 0.01 - 0.1:0.5 - 2.5.

[0048] Specifically, in this embodiment, the volume ratio of the liquid A to the liquid B can be 100:5 - 20.

[0049] It should be noted that the liquid B can also include the above-mentioned humectant, thickener, and antioxidant.

[0050] More preferably, in this embodiment, the liquid A can also include auxiliaries. Specifically, the auxiliaries can be pH regulators, fragrances, etc.

[0051] Preferably, the copper tripeptide composition with anti-wrinkle and repair functions of the present invention is a copper tripeptide essence with anti-wrinkle and repair functions, and its pH is 3 - 6, preferably 4 - 5.

[0052] The pH regulator is used to adjust the pH of the copper tripeptide essence with anti-wrinkle and repair functions to a suitable value. It should be noted that if the pH of the copper tripeptide essence with anti-wrinkle and repair functions is at a suitable value, there is no need to use a pH regulator.

[0053] The thickness of the polydopamine layer can be set according to actual needs. Preferably, in this embodiment, the thickness of the polydopamine layer is 1 nm to 50 nm.

[0054] Preferably, in this embodiment, the humectant is selected from at least one of glycerol and glucosylglycerol.

[0055] Preferably, in this embodiment, the thickener is selected from at least one of sodium carboxymethylcellulose, hydroxyethyl cellulose, and sodium alginate.

[0056] Preferably, in this embodiment, the antioxidant is selected from at least one of cysteine and ascorbic acid.

[0057] It should be noted that the antioxidant can also include various plant extracts with antioxidant functions.

[0058] Combined Figure 1 , the present invention also discloses an embodiment of the preparation method of the above-mentioned blue copper peptide composition with anti-wrinkle and repair functions, including the following steps:

[0059] S10. Prepare an aqueous solution of the thickener, disperse the humectant and antioxidant in water, then add the aqueous solution of the thickener, stir evenly and then add the blue copper peptide microcapsules to obtain Solution A.

[0060] Among them, the blue copper peptide microcapsules include blue copper peptide as the core and a polydopamine layer wrapped outside the blue copper peptide.

[0061] Preferably, in this embodiment, the blue copper peptide microcapsules are prepared by the following operations: adding a dopamine hydrochloride solution with a pH of 8-9 and a concentration of 1 mg / mL to 5 mg / mL to the blue copper peptide solution. After the dopamine hydrochloride is dissolved in water, it will hydrolyze to obtain dopamine. Stir and react for 12 h to 24 h, so that the hydrolyzed dopamine undergoes a self-polymerization reaction on the surface of the blue copper peptide to form a polydopamine layer wrapped outside the blue copper peptide, and blue copper peptide microcapsules are obtained.

[0062] Generally speaking, the above reaction can be carried out at normal temperature and pressure.

[0063] The thickness of the polydopamine layer can be set according to actual needs. During actual operation, the thickness of the obtained polydopamine layer can be achieved by adjusting the volume ratio of the blue copper peptide solution to the dopamine hydrochloride solution, the concentration of the dopamine hydrochloride solution, and the pH value.

[0064] Preferably, in this embodiment, the thickness of the polydopamine layer is 1 nm to 50 nm.

[0065] More preferably, in this embodiment, the volume ratio of the blue copper peptide solution to the dopamine hydrochloride solution is 1:0.5 to 2.

[0066] The concentration of the copper peptide solution can be set according to actual needs. For example, it can be 50 g / L to 300 g / L, preferably 100 g / L to 200 g / L.

[0067] Among them, the concentration of the copper peptide solution can be relatively high, so as to increase the proportion of copper peptide in the obtained copper peptide microcapsules. The residue obtained by filtration after the reaction is the copper peptide microcapsule, and the filtrate can be continuously used for the preparation of copper peptide microcapsules.

[0068] More preferably, in this embodiment, the concentration of the dopamine hydrochloride solution is 3 mg / mL.

[0069] Specifically, the solvent of the dopamine hydrochloride solution is Tris-HCl buffer solution. Dopamine in the dopamine hydrochloride solution is easily oxidized under alkaline conditions, for example, quinone substances are generated. Using Tris-HCl buffer solution as the solvent can effectively maintain the dopamine hydrochloride solution system at neutral to weakly alkaline, and can delay the oxidation process of dopamine. Compared with buffer solutions with high ionic strength, such as phosphate buffer solution, Tris-HCl buffer solution has a lower ionic strength and can reduce the influence on the charge state of dopamine.

[0070] Preferably, in this embodiment, by mass, solution A includes 0.5 to 2.5 parts of copper peptide microcapsules, 2 to 8 parts of humectant, 1.5 to 2.5 parts of thickener, 1 to 3 parts of antioxidant, and 80 to 120 parts of water.

[0071] More preferably, in this embodiment, solution A can also include additives. Specifically, the additives can be pH regulators, fragrances, etc.

[0072] Preferably, the copper peptide composition with anti-wrinkle and repair functions of the present invention is a copper peptide essence with anti-wrinkle and repair functions, and its pH is 3 to 6, preferably 4 to 5.

[0073] The pH regulator is used to adjust the pH of the copper peptide essence with anti-wrinkle and repair functions to a suitable value. It should be noted that if the pH of the copper peptide essence with anti-wrinkle and repair functions is at a suitable value, there is no need to use a pH regulator.

[0074] Preferably, in this embodiment, the humectant is selected from at least one of glycerol and glucosylglycerol.

[0075] Preferably, in this embodiment, the thickener is selected from at least one of sodium carboxymethyl cellulose, hydroxyethyl cellulose, and sodium alginate.

[0076] Preferably, in this embodiment, the antioxidant is selected from at least one of cysteine and ascorbic acid.

[0077] It should be noted that the antioxidant can also be various plant extracts with antioxidant functions.

[0078] S20. Dissolve the polydopamine decomposer in water to obtain solution B.

[0079] Among them, the polydopamine decomposer is used to decompose the polydopamine layer wrapped outside the copper peptide, and the polydopamine decomposer does not decompose the copper peptide.

[0080] Combining solution A and solution B can obtain the copper peptide composition with anti-wrinkle and repair functions of the present invention.

[0081] Preferably, in this embodiment, the polydopamine decomposer is peroxidase.

[0082] Polydopamine contains a large number of catechol (polyphenol) structures, which are easily recognized and catalytically oxidized by peroxidase. After the polyphenol structure is catalytically oxidized, the molecular structure of polydopamine will break or recombine, so that the polydopamine film of the copper peptide microcapsule ruptures, and then the internal copper peptide is released.

[0083] More preferably, in this embodiment, the peroxidase is lactoperoxidase.

[0084] Lactoperoxidase (LPO) is an enzyme naturally present in mammalian milk, saliva and tears. Lactoperoxidase extracted from natural sources (such as cow milk or plants) meets the needs of consumers for natural ingredients.

[0085] Polydopamine contains a large number of catechol (polyphenol) structures, which are easily recognized and catalytically oxidized by lactoperoxidase. After the polyphenol structure is catalytically oxidized, the molecular structure of polydopamine breaks or recombines, so that the polydopamine film ruptures, and then the internal copper peptide is released.

[0086] Lactoperoxidase has powerful antibacterial, antioxidant and anti-inflammatory properties.

[0087] Lactoperoxidase can scavenge free radicals, reduce the damage of oxidative stress to the skin, thus having an antioxidant effect, helping to delay skin aging, and improving wrinkles and fine lines.

[0088] Lactoperoxidase is also suitable for sensitive skin or inflammatory skin problems (such as eczema, rosacea).

[0089] Preferably, in this embodiment, the mass ratio of the polydopamine decomposer to the copper peptide microcapsule is 0.01 - 0.1:0.5 - 2.5.

[0090] Specifically, in this embodiment, the volume ratio of liquid A to liquid B can be 100:5 to 20.

[0091] It should be noted that liquid B can also include the above-mentioned moisturizing agent, thickening agent and antioxidant.

[0092] When the copper peptide blue copper peptide composition with anti-wrinkle and repair function prepared by the preparation method of the present invention is stored, the copper peptide blue copper can be protected by wrapping the copper peptide blue copper with a polydopamine layer, avoiding the hydrolysis of the peptide and the premature release of copper ions caused by the dissolution of the copper peptide blue copper under acidic conditions, and improving the bioavailability of the copper peptide blue copper.

[0093] Before using the copper peptide blue copper peptide composition with anti-wrinkle and repair function prepared by the preparation method of the present invention, it is necessary to mix liquid B including a polydopamine decomposer with liquid A including copper peptide blue copper. The dopamine decomposer is used to decompose the polydopamine layer wrapped outside the copper peptide blue copper, and the dopamine decomposer does not decompose the copper peptide blue copper. Thus, after mixing liquid B and liquid A, the polydopamine layer can be destroyed, and the copper peptide blue copper can be released, avoiding the hydrolysis of the peptide and the premature release of copper ions caused by the premature dissolution of the copper peptide blue copper in the liquid phase.

[0094] It should be noted that before using the copper peptide blue copper peptide composition with anti-wrinkle and repair function prepared by the preparation method of the present invention, it is necessary to mix liquid B including a polydopamine decomposer with liquid A including copper peptide blue copper. Therefore, in product design, liquid A can be stored in a large container, and liquid B can be filled in a small container and then stored in the large container. When the product is opened, the small container is opened at the same time, so as to mix liquid A and liquid B. After mixing, it is necessary to shake and mix and let it stand for 24 h to 48 h before use.

[0095] The following are specific examples.

[0096] In the specific example, the copper peptide blue copper is purchased from Wuhan Dongkangyuan Technology Co., Ltd., lactoperoxidase is purchased from Merck Biotech, dopamine hydrochloride is purchased from Shanghai Yuanye Bio-Technology Co., Ltd., the water in the examples is deionized water, and other reagents and equipment used are conventional products in the art.

[0097] Example 1

[0098] Under normal temperature and pressure, 30 g of copper peptide blue copper is dissolved in 130 mL of water to obtain a copper peptide blue copper solution. Then, the copper peptide blue copper solution is added to a Tris-HCl solution (pH = 9, concentration of dopamine hydrochloride = 3 mg / mL) of 200 mL of dopamine hydrochloride solution, and stirred and reacted for 16 h, so that the dopamine hydrolyzed in the dopamine hydrochloride solution undergoes a self-polymerization reaction on the surface of the copper peptide blue copper, forming a polydopamine layer wrapped outside the copper peptide blue copper. The filter residue is filtered and collected, washed 3 times with water and then vacuum dried to obtain copper peptide blue copper microcapsules.

[0099] Pre-disperse 2 g of sodium carboxymethylcellulose with 5 g of glycerol and then dissolve it in 60 mL of water. Then dissolve 2 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 1 g of the prepared copper peptide microcapsules to obtain Solution A.

[0100] Dissolve 0.05 g of lactoperoxidase in 10 mL of water to obtain Solution B.

[0101] Example 2

[0102] Pre-disperse 1.5 g of sodium carboxymethylcellulose with 8 g of glycerol and then dissolve it in 60 mL of water. Then dissolve 1 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 2.5 g of the copper peptide microcapsules prepared in Example 1 to obtain Solution A.

[0103] Dissolve 0.1 g of lactoperoxidase in 10 mL of water to obtain Solution B.

[0104] Example 3

[0105] Pre-disperse 2.5 g of sodium carboxymethylcellulose with 2 g of glycerol and then dissolve it in 60 mL of water. Then dissolve 3 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 0.5 g of the copper peptide microcapsules prepared in Example 1 to obtain Solution A.

[0106] Dissolve 0.01 g of lactoperoxidase in 10 mL of water to obtain Solution B.

[0107] Example 4

[0108] Pre-disperse 2 g of sodium carboxymethylcellulose with 5 g of glycerol and then dissolve it in 60 mL of water. Then dissolve 2 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 0.5 g of the copper peptide microcapsules prepared in Example 1 to obtain Solution A.

[0109] Dissolve 0.1 g of lactoperoxidase in 5 mL of water to obtain Solution B.

[0110] Example 5

[0111] Pre-disperse 2 g of sodium carboxymethylcellulose with 5 g of glycerol and then dissolve it in 60 mL of water. Then dissolve 2 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 2.5 g of the copper peptide microcapsules prepared in Example 1 to obtain Solution A.

[0112] Dissolve 0.01 g of lactoperoxidase in 20 mL of water to obtain Solution B.

[0113] Comparative Example 1

[0114] Dissolve 2 g of sodium carboxymethylcellulose by pre-dispersing it with 5 g of glycerol and then dissolving it in 60 mL of water. Next, dissolve 2 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 1 g of copper peptide GHK to obtain the product.

[0115] Comparative Example 2

[0116] Dissolve 2 g of sodium carboxymethylcellulose by pre-dispersing it with 5 g of glycerol and then dissolving it in 60 mL of water. Next, dissolve 0.1 g of lactoperoxidase and 2 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 1 g of copper peptide GHK to obtain the product.

[0117] Comparative Example 3

[0118] Dissolve 1.5 g of sodium carboxymethylcellulose by pre-dispersing it with 8 g of glycerol and then dissolving it in 60 mL of water. Next, dissolve 1 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 2.5 g of copper peptide GHK to obtain the product.

[0119] Comparative Example 4

[0120] Dissolve 2.5 g of sodium carboxymethylcellulose by pre-dispersing it with 2 g of glycerol and then dissolving it in 60 mL of water. Next, dissolve 3 g of ascorbic acid in 40 mL of water. After mixing the two and stirring evenly, add 0.5 g of copper peptide GHK to obtain the product.

[0121] Test Example

[0122] Send the Liquid A and Liquid B prepared in Examples 1 - 3 and the products prepared in Comparative Examples 1 - 3 to a third party (a certain city's Institute for Drug Control) after storing them for 6 months. Respectively, conduct concentration tests on the Liquid A prepared in Examples 1 - 3, the mixture of Liquid A and Liquid B prepared in Examples 1 - 3 (test after 24 hours of mixing), and the products prepared in Comparative Examples 1 - 3 for copper peptide GHK (Tripeptide-1 Copper, GHK-Cu) and Tripeptide-1 (GHK). The test results are shown in Table 1 below.

[0123]

[0124]

[0125] As can be seen from Table 1, after 6 months of storage, the blue copper peptide in the products prepared in Comparative Examples 1, 3, and 4 showed obvious decomposition, and a large amount of tripeptide-1 was produced. After 6 months of storage, there was almost no blue copper peptide and tripeptide-1 in the Solution A prepared in Examples 1 to 3, indicating that after the blue copper peptide was encapsulated by the polydopamine layer, the blue copper peptide was well protected. In addition, after 6 months of storage, the contents of the blue copper peptide and tripeptide-1 detected in the products prepared in Comparative Examples 1 and 2 were basically the same, indicating that lactoperoxidase hardly decomposed the blue copper peptide. A large amount of blue copper peptide could be detected in the mixed solution of Solution A and Solution B prepared in Examples 1 to 3, indicating that lactoperoxidase could destroy the polydopamine layer, thereby releasing the blue copper peptide. In addition, in the mixed solution of Solution A and Solution B prepared in Examples 1 to 3, the blue copper peptide still accounted for the vast majority, and a small amount of tripeptide-1 appeared. The possible reasons are, on the one hand, the product purity problem, and on the other hand, a small amount of decomposition may also have occurred in the blue copper peptide stored in the polydopamine layer.

[0126] In the description of the embodiments of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the embodiments of the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the embodiments of the present invention. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0127] In the description of the embodiments of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0128] In the embodiments of the present invention, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature may mean that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may mean that the first feature is directly above or obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature may mean that the first feature is directly below or obliquely below the second feature, or merely indicates that the horizontal height of the first feature is less than that of the second feature.

[0129] In the description of this specification, the description referring to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the embodiments of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0130] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A copper tripeptide composition with anti-wrinkle and repair functions, characterized in that, Comprising: Solution A and Solution B; Solution A includes copper tripeptide microcapsules, a humectant, a thickener, an antioxidant, and water. The copper tripeptide microcapsules include copper tripeptide as the core and a polydopamine layer wrapped around the copper tripeptide; Solution B includes a polydopamine decomposer and water. The polydopamine decomposer is used to decompose the polydopamine layer wrapped around the copper tripeptide, and the polydopamine decomposer does not decompose the copper tripeptide.

2. The copper peptide composition with anti-wrinkle and repair functions according to claim 1, wherein, The polydopamine decomposer is peroxidase.

3. The copper peptide composition with anti-wrinkle and repair functions according to claim 2, wherein The peroxidase is lactoperoxidase.

4. The copper peptide composition with anti-wrinkle and repair functions according to claim 3, characterized in that, By mass fraction, Solution A includes 0.5 parts to 2.5 parts of the copper tripeptide microcapsules, 2 parts to 8 parts of the humectant, 1.5 parts to 2.5 parts of the thickener, 1 part to 3 parts of the antioxidant, and 80 parts to 120 parts of water. The mass ratio of the polydopamine decomposer to the copper tripeptide microcapsules is 0.01 - 0.1:0.5 - 2.

5.

5. The copper peptide composition with anti-wrinkle and repair functions according to any one of claims 1 to 4, characterized in that The thickness of the polydopamine layer is 1nm to 50nm.

6. The copper peptide composition with anti-wrinkle and repair functions according to claim 5, wherein The humectant is selected from at least one of glycerol and glucosylglycerol.

7. The copper peptide composition with anti-wrinkle and repair functions according to claim 6, characterized in that, The thickener is selected from at least one of sodium carboxymethylcellulose, hydroxyethyl cellulose, and sodium alginate.

8. The copper tripeptide composition with anti-wrinkle and repair functions according to claim 6, characterized in that The antioxidant is selected from at least one of cysteine and ascorbic acid.

9. A method for preparing a blue copper peptide composition with anti-wrinkle and repair functions as described in any one of claims 1 to 8, characterized in that, Including the following steps: Prepare an aqueous solution of the thickener, disperse the humectant and the antioxidant in water, then add the aqueous solution of the thickener, and after stirring evenly, add the copper tripeptide microcapsules to obtain Solution A, wherein the copper tripeptide microcapsules include copper tripeptide as the core and a polydopamine layer wrapped around the copper tripeptide; Dissolve the polydopamine decomposer in water to obtain Solution B, wherein the polydopamine decomposer is used to decompose the polydopamine layer wrapped around the copper tripeptide, and the polydopamine decomposer does not decompose the copper tripeptide.

10. The preparation method of the copper tripeptide composition with anti-wrinkle and repair functions according to claim 9, characterized in that, The copper tripeptide microcapsules are prepared through the following operations: Add a dopamine hydrochloride solution with a pH of 8 - 9 and a concentration of 1mg / mL - 5mg / mL to the copper tripeptide solution, stir and react for 12h - 24h to cause the dopamine to undergo self-polymerization reaction to form the polydopamine layer wrapped around the copper tripeptide, thereby obtaining the copper tripeptide microcapsules.

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