A bacteriostatic composition and its use in hand sanitizer

Abstract

The application discloses a bacteriostatic composition and application thereof in hand sanitizer, and the bacteriostatic composition comprises the following components in percentage by mass: lysozyme 3-5%, enzyme protection system 4-8%, plant bacteriostatic extract 8-12%, biological matrix carrier 10-15%, stable protection system 5-8%, and the balance is water. The bacteriostatic composition can form a protective layer with good biocompatibility on the skin surface through a three-dimensional network structure formed by a carboxymethyl chitosan-sodium alginate compound, and can significantly prolong the skin retention time of lysozyme and plant bacteriostatic active ingredients. In the application, lysozyme and plant bacteriostatic ingredients are used to form a multiple bacteriostatic mechanism: lysozyme specifically hydrolyzes peptidoglycan in the bacterial cell wall, and active ingredients in the plant bacteriostatic extract destroy the bacterial cell membrane and inhibit bacterial metabolism. The double attack mechanism has a stronger bacteriostatic effect than a single component, and can prevent bacteria from developing drug resistance.

Description

Technical Field

[0001] This invention relates to the technical field of hand sanitizers, and more specifically to an antibacterial composition and its application in hand sanitizers. Background Technology

[0002] With increasing awareness of personal hygiene, hand sanitizer has become widely used as a convenient and effective hand cleaning and antibacterial product. Currently, hand sanitizers on the market are mainly divided into two categories: general cleaning and antibacterial. Antibacterial hand sanitizers typically use chemically synthesized antibacterial agents as their main active ingredients, such as triclosan and quaternary ammonium compounds (such as benzalkonium bromide). While these chemical antibacterial ingredients have a strong immediate antibacterial effect, they have the following significant drawbacks: First, most of the chemical antibacterial agents are washed away with water after handwashing, leaving little residue and resulting in poor antibacterial durability; second, long-term use may lead to adverse reactions such as dry skin and allergies; third, some chemical antibacterial agents, such as triclosan, have been proven to potentially disrupt the endocrine system and promote the development of drug-resistant strains, and their use has been restricted or banned in many countries and regions; finally, these compounds have poor biodegradability and pose potential environmental hazards.

[0003] In recent years, the application of natural antibacterial ingredients, such as plant extracts and enzymes like lysozyme, in antibacterial products has gradually increased. Polyphenols, flavonoids, and terpenes in plant extracts possess broad-spectrum antibacterial activity and are gentle on humans and environmentally friendly. Lysozyme, a hydrolytic enzyme widely found in nature, can specifically hydrolyze peptidoglycan components in bacterial cell walls, disrupting cell structure and leading to bacterial death. It also boasts advantages such as high safety and low likelihood of inducing drug resistance.

[0004] However, existing hand sanitizers based on natural antibacterial ingredients still face many technical challenges: lysozyme, as a protein enzyme, has poor stability in the hand sanitizer formulation environment, and its activity is easily reduced by pH, temperature, and chemical substances; natural antibacterial ingredients have short contact time with the skin, making it difficult to form an effective and long-lasting antibacterial effect; and the antibacterial spectrum of a single antibacterial ingredient is limited, making it difficult to cover a variety of common pathogens.

[0005] The currently published patent document CN113967251A discloses an antibacterial agent containing lysozyme, which is composed of plant extracts, lysozyme, pine oil, benzalkonium bromide, and a penetration enhancer. This technology utilizes pine oil to form a thin film on the skin surface, prolonging the residence time of the various antibacterial active substances and thus achieving a better, longer-lasting antibacterial effect. However, this technology still has significant shortcomings: it still relies on chemical antibacterial agents such as benzalkonium bromide; it does not provide an effective solution to the stability problem of lysozyme; and the film formed by pine oil may cause an uncomfortable greasy feeling for the user.

[0006] Therefore, developing an antibacterial composition that is free of chemical antibacterial agents, has a long-lasting antibacterial effect, and can effectively protect the activity of lysozyme has become an important research and development direction in the field of hand sanitizer technology. Summary of the Invention

[0007] In view of the problems existing in the prior art, the present invention provides an innovative antibacterial composition based on lysozyme and natural plant extracts and its application in hand sanitizer.

[0008] This invention is implemented through the following technical solutions:

[0009] The first aspect of this invention discloses an antibacterial composition comprising, by weight percentage: 3-5% lysozyme, 4-8% enzyme protection system, 8-12% plant antibacterial extract, 10-15% biological matrix carrier, 5-8% stabilization protection system, and the balance being water.

[0010] Furthermore, the enzyme protection system includes cofactors, enzyme stabilizers, and antioxidants;

[0011] The mass ratio of cofactor, enzyme stabilizer, and antioxidant is (0.3-0.5):(3-6):(0.3-0.5).

[0012] Among them, the cofactors are magnesium ions and zinc ions, and the molar ratio of magnesium ions to zinc ions is 2:1;

[0013] The enzyme stabilizers are mannitol and trehalose, with a mass ratio of mannitol to trehalose of (1-2.5):1;

[0014] The antioxidant is a mixture of glutathione and disodium EDTA in a mass ratio of 1:0.2.

[0015] This invention designs a multi-layered protection system for lysozyme, including structural stability protection, antioxidant protection, and microenvironment regulation, enabling lysozyme to maintain high activity in complex formulation environments. Mannitol and trehalose act as permeation protectants, stabilizing the tertiary structure of lysozyme; glutathione and vitamin E acetate form an antioxidant network, inhibiting protein oxidation; while cofactors (magnesium ions and zinc ions) maintain the enzyme's catalytic activity.

[0016] Furthermore, the plant antibacterial extracts include green tea extract, pomegranate peel extract, grape seed extract, rosemary extract, and lemon extract;

[0017] The mass ratio of green tea extract, pomegranate peel extract, grape seed extract, rosemary extract and lemon extract is (2-3):(1.5-2.5):(1.5-2.5):(1.5-2.5):(1-2).

[0018] Furthermore, the bio-matrix carrier comprises a carboxymethyl chitosan-sodium alginate complex, hyaluronic acid, glycerol, and betaine;

[0019] The mass ratio of carboxymethyl chitosan-sodium alginate complex, hyaluronic acid, glycerol and betaine is (4-8):(1-2):(2-3):(2-4).

[0020] Furthermore, the stabilizing protection system includes a lipid-soluble antioxidant, panthenol, a pH buffer solution, and an emulsifier;

[0021] The mass ratio of fat-soluble antioxidant, panthenol, pH buffer system and emulsifier is (1.5-2.5):(1-2):(2-3):(0.2-0.8);

[0022] The fat-soluble antioxidant is vitamin E acetate; the pH buffer solution is a lactic acid / sodium lactate buffer solution.

[0023] Furthermore, the pH value of the antibacterial composition is 6.5-7.0.

[0024] A second aspect of the present invention discloses a method for using the antibacterial composition, comprising the following steps:

[0025] (1) Preparation of biological matrix carrier: Dissolve carboxymethyl chitosan in water, dissolve sodium alginate in water, slowly add sodium alginate solution to carboxymethyl chitosan solution, mix evenly, add hyaluronic acid, betaine and glycerol in sequence, mix evenly to obtain biological matrix carrier;

[0026] (2) Preparation of lysozyme stabilization system: Dissolve the enzyme stabilizer in phosphate buffer at pH 6.3, adjust the temperature to 20±2℃, add lysozyme, cofactor and antioxidant, and stir at 50-100 rpm until uniform to obtain lysozyme stabilization system;

[0027] (3) Preparation of plant antibacterial extract stabilization system: Emulsifier and fat-soluble antioxidant are added to ethanol-water mixed solution, and after dissolution, plant antibacterial extract components are added in sequence. After mixing evenly, the mixture is concentrated under reduced pressure to remove ethanol and obtain plant antibacterial extract stabilization system.

[0028] (4) Add the lysozyme stabilization system obtained in step (2) to the biological matrix carrier obtained in step (1) and stir evenly;

[0029] (5) Add the plant antibacterial extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, add panthenol and pH buffer solution, adjust the pH value, homogenize and emulsify, and then fill to obtain the final antibacterial composition.

[0030] A third aspect of the present invention discloses the application of the antibacterial composition in a hand sanitizer, the hand sanitizer comprising the following components in weight percentage: 1-3% antibacterial composition, 2.5-3.5% dodecyl glucoside, 1.5-2.5% betaine, 2-3% glycerin, 0.5-1% panthenol, 0.5-0.8% thickener, 0.2-0.4% lactic acid / sodium lactate buffer solution, 0.3-0.5% preservative, 0.05-0.1% fragrance, and the balance being water.

[0031] Furthermore, the thickener is xanthan gum; the preservative is benzyl alcohol and / or phenoxyethanol.

[0032] Furthermore, the pH value of the hand sanitizer is 6.5-7.0.

[0033] The beneficial effects of this invention are:

[0034] 1. The antibacterial composition of the present invention, through a three-dimensional network structure formed by a carboxymethyl chitosan-sodium alginate complex, can form a biocompatible protective layer on the skin surface, significantly prolonging the skin retention time of lysozyme and plant antibacterial active ingredients. Unlike traditional hand sanitizers whose antibacterial activity rapidly declines after rinsing, the hand sanitizer of the present invention maintains an antibacterial rate of over 85% for up to 8 hours after use. The present invention employs a multi-layered antibacterial mechanism combining lysozyme and plant antibacterial ingredients: lysozyme specifically hydrolyzes peptidoglycan in bacterial cell walls, while plant active ingredients such as green tea extract, pomegranate peel extract, and grape seed extract disrupt bacterial cell membranes and inhibit bacterial metabolism. This dual-attack mechanism has a stronger antibacterial effect than a single component and also prevents bacteria from developing drug resistance.

[0035] 2. The antibacterial composition of this invention is derived from natural substances or biocompatible materials, has a skin irritation index of <0.5, and possesses moisturizing and skin-care properties. Long-term use will not cause skin dryness or irritation, thus solving the adverse effects of traditional antibacterial hand sanitizers on the skin. This invention completely eliminates chemical antibacterial agents such as triclosan and benzalkonium bromide. All components have good biodegradability, are environmentally friendly, and conform to the concept of green and sustainable development. Detailed Implementation

[0036] The technical solution of the present invention will be further described in detail below with reference to specific embodiments, but the scope of protection of the present invention is not limited to the following embodiments.

[0037] Example of preparation of antibacterial agent

[0038] Preparation Example 1

[0039] An antibacterial composition comprising, by weight percentage:

[0040] Lysozyme 4.0%

[0041] Enzyme protection system 6.0%: cofactor (magnesium sulfate, zinc sulfate, molar ratio 2:1) 0.38%; enzyme stabilizer (mannitol, trehalose, mass ratio 2:1) 5.0%; antioxidant protectant (glutathione, disodium EDTA, mass ratio 1:0.2) 0.42%;

[0042] Plant antibacterial extracts 10.0%: green tea extract 2.5%, pomegranate peel extract 2.0%, grape seed extract 2.0%, rosemary extract 2.0%, lemon extract 1.5%;

[0043] Biological matrix carrier 12.0%: carboxymethyl chitosan-sodium alginate complex 6.0%, hyaluronic acid 1.2%, glycerol 2.3%, betaine 2.5%;

[0044] The stabilizing and protective system consists of: 1.8% vitamin E acetate, 1.5% panthenol, 2.5% lactic acid / sodium lactate buffer solution, and 0.4% emulsifier (polysorbate 80).

[0045] Bring the purified water level to 100%.

[0046] A method for using an antibacterial composition includes the following steps:

[0047] (1) Preparation of biological matrix carrier: Dissolve carboxymethyl chitosan (4.5%) in water, dissolve sodium alginate (1.5%) in water, slowly add sodium alginate solution to carboxymethyl chitosan solution, mix evenly, then add hyaluronic acid, betaine and glycerol in sequence, stir at 500 rpm for 40 minutes to mix evenly, and obtain biological matrix carrier.

[0048] (2) Preparation of lysozyme stabilization system: Dissolve mannitol and trehalose in phosphate buffer at pH 6.3, adjust the temperature to 20±2℃, add lysozyme, magnesium sulfate, zinc sulfate, glutathione and disodium EDTA, stir at 80 rpm for 60 minutes until uniform, and obtain lysozyme stabilization system.

[0049] (3) Preparation of plant antibacterial extract stabilization system: Polysorbate 80 and vitamin E acetate were added to an ethanol-water (7:3) mixed solution and dissolved. Green tea extract, pomegranate peel extract, grape seed extract, rosemary extract and lemon extract were added in sequence. After mixing evenly in a 40°C water bath, the mixture was concentrated under reduced pressure at 45°C to remove ethanol and obtain plant antibacterial extract stabilization system.

[0050] (4) Add the lysozyme stabilization system obtained in step (2) to the biological matrix carrier obtained in step (1) and stir at 400 rpm for 30 minutes until homogeneous;

[0051] (5) Add the plant antibacterial extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, add panthenol and lactic acid / sodium lactate buffer solution, adjust the pH value to 6.8, homogenize at 6000 rpm for 5 minutes and then fill to obtain the final antibacterial composition.

[0052] Preparation Example 2

[0053] An antibacterial composition comprising, by weight percentage:

[0054] Lysozyme 4.5%

[0055] Enzyme protection system 6.92%; cofactor (magnesium sulfate, zinc sulfate, molar ratio 2:1) 0.44%; enzyme stabilizer (mannitol, trehalose, mass ratio 2:1) 6.0%; antioxidant protectant (glutathione, disodium EDTA, mass ratio 1:0.2) 0.48%.

[0056] Plant antibacterial extracts 11.0%; green tea extract 2.8%, pomegranate peel extract 2.2%, grape seed extract 2.2%, rosemary extract 2.2%, lemon extract 1.6%;

[0057] Biological matrix carrier 14.5%: carboxymethyl chitosan-sodium alginate complex 7.0% (carboxymethyl chitosan 5.0%, sodium alginate 2.0%), hyaluronic acid 1.8%, glycerol 2.5%, betaine 3.2%;

[0058] The stabilizing and protective system consists of: vitamin E acetate 2.0%, panthenol 1.8%, lactic acid / sodium lactate buffer solution 2.8%, and emulsifier (polysorbate 80) 0.5%.

[0059] Bring the purified water level to 100%.

[0060] The preparation method of the antibacterial agent is the same as that in Preparation Example 1.

[0061] Comparative Preparation Example 1

[0062] Based on Preparation Example 1, this comparative preparation example does not include the enzyme protection system, but is otherwise the same as Preparation Example 1;

[0063] The specific method for preparing the antibacterial composition is as follows:

[0064] (1) Preparation of biological matrix carrier: Same as in preparation example 1;

[0065] (2) Preparation of lysozyme solution: Dissolve lysozyme directly in 200 mL of phosphate buffer at pH 6.3 without adding any enzyme protection system components;

[0066] (3) Preparation of a plant antibacterial extract stabilization system: Same as in Example 1;

[0067] (4) Add the lysozyme solution obtained in step (2) to the biological matrix carrier obtained in step (1) and stir at 400 rpm for 30 minutes until homogeneous;

[0068] (5) Add the plant antibacterial extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, and perform subsequent operations in the same manner as in Preparation Example 1 to obtain the final antibacterial composition.

[0069] Comparative Preparation Example 2

[0070] Based on Preparation Example 1, this comparative preparation example lacks plant antibacterial extracts;

[0071] The specific method for preparing the antibacterial composition is as follows:

[0072] (1) Preparation of biological matrix carrier: Same as in preparation example 1;

[0073] (2) Preparation of lysozyme stabilization system: Same as in Example 1;

[0074] (3) Add the lysozyme stabilization system to the biological matrix carrier and stir until homogeneous;

[0075] (4) Add vitamin E acetate and emulsifier to the mixture obtained in step (3), stir evenly, add panthenol and lactic acid / sodium lactate buffer solution, adjust the pH value to 6.8, and perform subsequent operations in the same way as in Preparation Example 1 to obtain the final antibacterial composition.

[0076] Comparative preparation example 3

[0077] Based on Preparation Example 1, this comparative preparation example uses hydroxypropyl methylcellulose as a carrier;

[0078] The specific method for preparing the antibacterial composition is as follows:

[0079] (1) Preparation of biological matrix carrier: 1.5% hydroxypropyl methylcellulose (HPMC) was dissolved in water, 3.0% glycerol was added, and the mixture was stirred evenly to obtain the carrier;

[0080] (2) Preparation of lysozyme stabilization system: Same as in Example 1;

[0081] (3) Preparation of a plant antibacterial extract stabilization system: Same as in Example 1;

[0082] (4) Add the lysozyme stabilization system obtained in step (2) to the carrier obtained in step (1) and stir at 400 rpm for 30 minutes until homogeneous;

[0083] (5) Add the plant antibacterial extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, and perform subsequent operations in the same manner as in Preparation Example 1 to obtain the final antibacterial composition.

[0084] Comparative preparation example 4

[0085] Based on Preparation Example 1, the types of plant antibacterial extracts included only green tea extract and pomegranate peel extract, omitting grape seed extract, rosemary extract and lemon extract;

[0086] The method for preparing the antibacterial composition is the same as in Preparation Example 1.

[0087] Antibacterial composition performance test

[0088] 1. Antibacterial activity test

[0089] The antibacterial effects of the antimicrobial compositions against Staphylococcus aureus, Escherichia coli, Candida albicans, and methicillin-resistant Staphylococcus aureus (MRSA) were determined using the plate diffusion method. Each test strain was inoculated onto a suitable culture medium plate, and wells (6 mm in diameter) were punched in each plate. 100 μL of the antimicrobial composition prepared in different formulations was added to each well. After incubation at 37°C for 24 hours, the diameter of the inhibition zone was measured. The specific results are shown in Table 1.

[0090] Table 1. Results of antibacterial activity test (diameter of inhibition zone, mm)

[0091] Group Staphylococcus aureus E. coli Candida albicans MRSA Preparation Example 1 18.5 16.8 15.4 16.2 Preparation Example 2 19.2 17.5 16.0 16.9 Comparative Preparation Example 1 12.6 11.5 10.2 9.8 Comparative Preparation Example 2 14.8 13.2 9.5 12.3 Comparative preparation example 3 15.9 14.7 13.5 13.5 Comparative preparation example 4 16.3 15.1 12.2 13.8

[0092] As shown in Table 1, Preparation Examples 1 and 2 exhibited significantly better antibacterial effects than the comparative examples. The antibacterial activity of Preparation Example 1 (lacking an enzyme protection system) was significantly reduced, indicating that the enzyme protection system is crucial for maintaining lysozyme activity. The inhibitory effect on fungi was significantly weakened in Preparation Example 2 (lacking plant antibacterial extracts), indicating that plant antibacterial extracts play an important role in combating fungal infections. The antibacterial effect of Comparative Example 3 (using a common carrier) was between that of the preparation examples and other comparative examples, indicating that a bio-based carrier can enhance antibacterial activity. Although Comparative Example 4 (with incomplete plant antibacterial extract composition) showed some inhibitory effect on common strains, its antibacterial effect against Candida albicans and MRSA was significantly less than that of the complete formulation, indicating that the synergistic effect of multiple plant extracts is crucial for broadening the antibacterial spectrum and enhancing antibacterial efficacy.

[0093] 2. Lysozyme activity stability test

[0094] The antibacterial compositions of each preparation example were stored at 40°C for 0, 30, 60, 90, and 180 days, and the residual lysozyme activity was measured. The initial activity was taken as 100%, and the relative activity percentage at each time point was calculated. Specific results are shown in Table 2.

[0095] Table 2. Results of lysozyme activity stability test (residual activity %) under storage conditions at 40℃

[0096] Group 0 days 30 days 60 days 90 days 180 days Preparation Example 1 100 92.5 85.3 78.6 70.3 Preparation Example 2 100 93.8 86.9 80.2 72.5 Comparative Preparation Example 1 100 65.3 42.6 25.8 12.4 Comparative Preparation Example 2 100 90.2 82.5 75.4 65.8 Comparative preparation example 3 100 83.7 70.4 58.9 45.2 Comparative preparation example 4 100 88.6 79.5 71.8 62.3

[0097] As shown in Table 2, Preparation Examples 1 and 2 exhibited excellent lysozyme activity retention under high-temperature storage conditions, maintaining approximately 80% activity after 90 days and over 70% activity after 180 days. Comparative Example 1, lacking an enzyme protection system, showed a rapid decline in lysozyme activity, retaining only 12.4% after 180 days. Comparative Example 3, using a conventional carrier instead of a biological matrix carrier, showed a significant decrease in lysozyme stability, indicating that the biological matrix carrier and enzyme protection system of this invention work synergistically to effectively protect lysozyme activity.

[0098] Example 1

[0099] A hand sanitizer containing an antibacterial composition, the hand sanitizer comprising the following components in weight percentage: 2.5% antibacterial composition, 3.0% dodecyl glucoside, 2.0% betaine, 2.5% glycerin, 0.7% panthenol, 0.6% xanthan gum, 0.28% lactic acid / sodium lactate buffer solution, 0.2% benzyl alcohol, 0.25% phenoxyethanol, 0.08% fragrance, and the balance being water.

[0100] The antibacterial composition used in this embodiment is the antibacterial composition prepared in Preparation Example 1.

[0101] The preparation method of hand sanitizer includes the following steps:

[0102] 1. Slowly add xanthan gum to a portion of purified water and stir at 600 rpm until completely dissolved to obtain a homogeneous thickened system;

[0103] 2. Add dodecyl glucoside, betaine, glycerol and panthenol to another portion of water, heat to 45°C, and stir until completely dissolved to obtain a surfactant system;

[0104] 3. Slowly add the surfactant system to the thickening system and stir at 400 rpm for 10 minutes until uniformly mixed; add the antibacterial composition and stir at 500 rpm for 15 minutes until uniformly dispersed; add lactic acid / sodium lactate buffer solution and adjust the pH to 6.8; add benzyl alcohol and phenoxyethanol as preservatives and stir until uniform; finally add the fragrance and stir until uniform; homogenize at 5000 rpm for 3 minutes, filter through a 0.45 μm filter membrane, and fill.

[0105] Example 2

[0106] The antibacterial composition used in this embodiment is the antibacterial composition prepared in Preparation Example 2; the preparation methods of the remaining components and hand sanitizer are the same as in Example 1.

[0107] Comparative Example 1

[0108] The antibacterial composition used in this comparative example is the antibacterial composition prepared in Comparative Preparation Example 1; the preparation methods of the remaining components and hand sanitizer are the same as in Example 1.

[0109] Comparative Example 2

[0110] The antibacterial composition used in this comparative example is the antibacterial composition prepared in Comparative Preparation Example 2; the preparation methods of the remaining components and hand sanitizer are the same as in Example 1.

[0111] Comparative Example 3

[0112] The antibacterial composition used in this comparative example is the antibacterial composition prepared in Comparative Preparation Example 3; the preparation methods of the remaining components and hand sanitizer are the same as in Example 1.

[0113] Comparative Example 4

[0114] The antibacterial composition used in this comparative example is the antibacterial composition prepared in Comparative Preparation Example 4; the preparation methods of the remaining components and hand sanitizer are the same as in Example 1.

[0115] Test case

[0116] Thirty volunteers were recruited. Each volunteer washed their right hand with the test sample and their left hand with regular soap as a control. Hand microbial samples were collected at 0, 2, 4, and 8 hours after handwashing, and the inhibition rate was calculated. The test results are shown in Table 3.

[0117] Table 3. Durability of antibacterial activity of hand sanitizer after handwashing (%)

[0118]

[0119]

[0120] As can be seen from the test results in Table 3, the hand sanitizers of Examples 1 and 2 maintained an antibacterial rate of over 85% for 8 hours after use, which was significantly better than the comparative examples and the control group of ordinary soap. Comparative Example 1, lacking an enzyme protection system, had unstable lysozyme activity, resulting in a significant decrease in antibacterial durability, especially after 4 and 8 hours. Comparative Example 2 lacked plant antibacterial extracts, resulting in a narrower antibacterial spectrum and less durability than the complete formula. Comparative Example 3 used a common carrier instead of a biological matrix carrier, reducing the retention time of the antibacterial components on the skin and affecting the long-lasting antibacterial effect. Although Comparative Example 4 contained some plant antibacterial extracts, its antibacterial durability was lower than that of Examples 1 and 2 due to incomplete composition.

[0121] Finally, it should be noted that the above embodiments are merely illustrative of several implementations of the present invention and are not intended to limit the scope of the invention. For those skilled in the art, any modifications, equivalent substitutions, or improvements made without departing from the concept of the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A bacteriostatic composition, characterized in that, By mass percentage, the following components are included: lysozyme 3-5%, enzyme protection system 4-8%, plant bacteriostatic extract 8-12%, biological matrix carrier 10-15%, stable protection system 5-8%, and the balance is water; The enzyme protection system includes auxiliary factors, enzyme stabilizers, and antioxidant protective agents, and the mass ratio of the auxiliary factors, enzyme stabilizers, and antioxidant protective agents is (0.3-0.5):(3-6):(0.3-0.5); The auxiliary factors are magnesium ions and zinc ions, the enzyme stabilizers are mannitol and trehalose, and the antioxidant protective agents are a mixture of glutathione and disodium EDTA; The plant bacteriostatic extract includes green tea extract, pomegranate peel extract, grape seed extract, rosemary extract, and lemon extract; The preparation method of the bacteriostatic composition includes the following steps: (1) Preparation of a biological matrix carrier: carboxymethyl chitosan is dissolved in water, sodium alginate is dissolved in water, and the sodium alginate solution is slowly added to the carboxymethyl chitosan solution. After mixing evenly, hyaluronic acid, betaine, and glycerol are added in sequence, and mixed evenly to obtain the biological matrix carrier; (2) Preparation of a lysozyme stabilization system: dissolve the enzyme stabilizer in a phosphate buffer solution at pH 6.3, adjust the temperature to 20±2℃, add lysozyme, auxiliary factors, and antioxidant protective agents, and stir at 50-100 rpm until uniform to obtain the lysozyme stabilization system; (3) Preparation of a plant bacteriostatic extract stabilization system: add emulsifiers and fat-soluble antioxidants to an ethanol-water mixed solution, dissolve, and then add the plant bacteriostatic extract components in sequence. After mixing evenly, remove ethanol under reduced pressure to obtain the plant bacteriostatic extract stabilization system; (4) Add the lysozyme stabilization system obtained in step (2) to the biological matrix carrier obtained in step (1) and stir evenly; (5) Add the plant bacteriostatic extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, add panthenol and a pH buffer solution, adjust the pH value, homogenize and emulsify, and then fill to obtain the final bacteriostatic composition.

2. The bacteriostatic composition according to claim 1, characterized in that, The molar ratio of magnesium ions to zinc ions is 2:1; The mass ratio of mannitol to trehalose is (1-2.5):1, and the mass ratio of glutathione to disodium EDTA is 1:0.

2.

3. The bacteriostatic composition of claim 1, wherein The mass ratio of green tea extract, pomegranate peel extract, grape seed extract, rosemary extract, and lemon extract in the plant bacteriostatic extract is (2-3):(1.5-2.5):(1.5-2.5):(1.5-2.5):(1-2).

4. The bacteriostatic composition of claim 1, wherein The biological matrix carrier includes a carboxymethyl chitosan-sodium alginate complex, hyaluronic acid, glycerol, and betaine; The mass ratio of the carboxymethyl chitosan-sodium alginate complex, hyaluronic acid, glycerol, and betaine is (4-8):(1-2):(2-3):(2-4).

5. The bacteriostatic composition of claim 1, wherein The stable protection system includes fat-soluble antioxidants, panthenol, a pH buffer solution, and emulsifiers; The mass ratio of the fat-soluble antioxidants, panthenol, pH buffer system, and emulsifiers is (1.5-2.5):(1-2):(2-3):(0.2-0.8). The fat-soluble antioxidant is vitamin E acetate; and the pH buffer solution is a lactic acid / sodium lactate buffer solution.

6. The bacteriostatic composition according to any one of claims 1 to 5, characterized in that, The pH value of the bacteriostatic composition is 6.5-7.

0.

7. Use of a bacteriostatic composition according to any one of claims 1 to 6 in a hand sanitizer, characterized in that, The hand washing liquid comprises the following components in percentage by weight: 1-3% of the bacteriostatic composition, 2.5-3.5% of dodecyl glucoside, 1.5-2.5% of betaine, 2-3% of glycerol, 0.5-1% of panthenol, 0.5-0.8% of thickening agent, 0.2-0.4% of lactic acid / sodium lactate buffer solution, 0.3-0.5% of preservative, 0.05-0.1% of essence, and the balance being water.

8. Use according to claim 7, characterized in that, The thickening agent is xanthan gum; and the preservative is benzyl alcohol and / or phenoxyethanol.

9. Use according to claim 7, characterized in that, The pH value of the hand washing liquid is 6.5-7.0.

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