A composition containing high mountain plant extract and ergothioneine and application thereof

Abstract

The present application provides a composition containing high mountain plant extracts and ergothioneine, comprising 0.005-0.8 parts by weight of Gentiana rigescens Franch. extract, 0.01-0.03 parts by weight of Gentiana rigescens Franch. extract, 0.1-0.4 parts by weight of Ledum palustre L. extract, 0.0005-0.005 parts by weight of Rhododendron anthopogon D.Don extract and 0.001-0.15 parts by weight of ergothioneine. The present application also provides a skin care product containing the composition containing high mountain plant extracts and ergothioneine. The composition and the skin care product containing the composition have excellent skin care effects of anti-photoaging, soothing and anti-oxidation.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and in particular to a composition containing alpine plant extracts and ergothioneine and its application. Background Technology

[0002] Photoaging is the aging-related changes in the skin caused by long-term cumulative sun exposure. Ultraviolet (UV) radiation penetrates the epidermis to reach the papillary dermis, leading to the formation of sunburned cells in the epidermis. UV radiation can cause direct DNA damage by promoting the formation of cyclopyrimidine dimers; it can also promote the formation of other photoproducts associated with skin cancer and skin aging, such as reactive oxygen species, matrix metalloproteinases, and the inflammatory factor IL-6. Once the skin is exposed to UV radiation, multiple signaling pathways in skin cells responsible for promoting photoaging and skin cancer by regulating cell growth, proliferation, and survival are activated, leading to changes in the skin. In dermal cells such as fibroblasts, UV radiation inhibits type I procollagen synthesis. Simultaneously, the overexpression of matrix metalloproteinases leads to a decrease in collagen and elastin levels, degrading structural proteins in the dermis that maintain skin elasticity.

[0003] Based on the principle of physical sun protection, this method uses materials such as titanium dioxide and zinc oxide to reflect sunlight, achieving a sun protection effect. The quality of these products depends on the quality of the reflective particles' processing; generally, ultraviolet rays can still penetrate the gaps between the particles to reach the skin. However, this method has the following drawbacks: the products contain a large number of reflective particles, which can easily clog pores; they are generally quite thick and heavy, making them unsuitable for sensitive and acne-prone skin; makeup removal is required after use, which is inconvenient; they are generally only used when outdoors and can only protect against some UV damage; and they only passively protect against light pollution and photoaging.

[0004] This method utilizes chemical UV absorbers to combat light pollution by reflecting and absorbing harmful ultraviolet rays. Since chemical sunscreen molecules are absorbed by the skin, the absorption process occurs within the skin and is eliminated through metabolism. However, this method has the following drawbacks: chemical sunscreens can irritate the skin; the products have a high oil content, which can easily lead to acne; they are generally not suitable for sensitive or acne-prone skin; makeup removal is required after use, which is inconvenient; they are typically only used outdoors, limiting their application; and they only passively protect against light pollution and photoaging.

[0005] Blue light absorbers such as calendula extract, sophora japonica extract, and hesperidin are used to prevent blue light from sunlight and LEDs from reaching the skin and causing damage. However, this method has the following drawbacks: it is only suitable for protecting against LED blue light, cannot resist photodamage caused by sunlight, can only passively resist LED blue light damage, and cannot actively resist photoaging caused by LED blue light.

[0006] Therefore, it is still necessary to develop a composition that can protect against light pollution, soothe and suppress inflammation from within the skin, and actively combat photoaging. Summary of the Invention

[0007] To solve at least one of the above-mentioned technical problems, this application provides a composition containing alpine plant extracts and ergothioneine, comprising: 0.005-0.8 parts by weight of saffron extract, 0.01-0.03 parts by weight of Scutellaria baicalensis extract, 0.1-0.4 parts by weight of Edelweiss extract, 0.0005-0.005 parts by weight of Rhododendron rose extract, and 0.001-0.15 parts by weight of ergothioneine.

[0008] Through the above technical solution, this application uses the growth environment of numerous plant components as a screening approach. High altitude—pure and less polluted; high sunshine—strong anti-ultraviolet ability. Alpine plants are selected as the main components of the anti-photoaging and antioxidant composition. Alpine plant extracts are screened for synergistic effects through combination. Saffron extract, Scutellaria baicalensis flower / leaf / stem extract, Edelweiss extract, Rhododendron rose extract, ergothioneine, and other components work synergistically through different pathways to significantly enhance skincare effects. Saffron extract is rich in antioxidants (crocin and saffron aldehyde), which can effectively neutralize free radicals. Crocin and saffron aldehyde have strong antioxidant properties, inhibiting lipid peroxidation, protecting cell membranes and collagen from oxidative damage, and promoting skin microcirculation and enhancing the skin's self-repair ability. Scutellaria baicalensis extract has anti-inflammatory and soothing effects, reducing skin inflammation and enhancing skin barrier function by inhibiting the release of inflammatory mediators. Edelweiss extract is renowned for its powerful moisturizing and anti-aging properties. Rich in flavonoids, phenolic acids, polysaccharides, and terpenes, it reduces oxidative stress damage to skin cells by neutralizing free radicals (such as reactive oxygen species) generated after UV radiation. It also promotes DNA repair by inhibiting matrix metalloproteinases (MMPs) in the skin, reducing UV damage to cellular genetic material. Alpine rose and rhododendron extract further enhances its antioxidant and anti-inflammatory effects while promoting collagen synthesis and improving skin elasticity. Ergothioneine, a highly effective antioxidant, protects cells from UV damage and other environmental factors, delaying skin aging. These components work synergistically through multiple pathways including antioxidant, anti-inflammatory, moisturizing, and collagen synthesis promotion. They effectively resist environmental damage to the skin, deeply nourish and repair it, achieving superior skincare results and leaving skin healthier, younger, and more radiant. Within this system, saffron extract and edelweiss extract exhibit significant synergistic effects in improving skin's anti-photoaging, soothing, and antioxidant properties.

[0009] Preferably, the composition containing alpine plant extracts and ergothioneine comprises: 0.4-0.8 parts by weight of saffron extract, 0.01-0.03 parts by weight of Scutellaria baicalensis extract, 0.1-0.4 parts by weight of Edelweiss extract, 0.0005-0.005 parts by weight of Rhododendron rose extract, and 0.01-0.1 parts by weight of ergothioneine.

[0010] Preferably, the composition containing alpine plant extract and ergothioneine further includes a penetration enhancer comprising 0.000001-0.00001 parts by weight of decapeptide-4 and 0.000001-0.00001 parts by weight of glycoprotein.

[0011] Through the aforementioned technical solutions, decapeptide-4 helps restore skin firmness and elasticity by promoting the production of collagen and elastin. Glycoproteins, on the other hand, enhance skin's moisturizing ability and barrier function by strengthening intercellular adhesion.

[0012] Preferably, the weight ratio of the saffron extract to the edelweiss extract is (1-8):1.

[0013] By using the above technical solution, when the weight ratio of saffron extract and edelweiss extract is controlled within a certain range, saffron extract and edelweiss extract can exert greater performance advantages in this system, achieving better anti-photoaging, soothing and whitening effects.

[0014] In a second aspect of this application, this application provides the use of the above-mentioned composition containing alpine plant extracts and ergothioneine in the preparation of anti-photoaging, soothing, and whitening cosmetics or skin care products.

[0015] In a third aspect of this application, this application provides a skin care product comprising the above-mentioned composition containing alpine plant extracts and ergothioneine, wherein the skin care product is any one of liquid, lotion, cream, oil or powder.

[0016] Preferably, the composition containing alpine plant extracts and ergothioneine is 0.9405-1.0116% by mass in the skin care product.

[0017] Preferably, the skincare product further comprises at least one of a chelating agent, a moisturizer, a thickener, an emulsifier, a skin-nourishing agent, a preservative, a solvent, and a pH adjuster; the chelating agent is disodium EDTA or tetrasodium EDTA; the moisturizer is selected from at least one of butylene glycol, glycerin, propylene glycol, and vegetable oil; the thickener is selected from ammonium acryloyl dimethyl taurate / VP copolymer, sodium polyacryloyl dimethyl taurate, sodium acrylate / sodium acryloyl dimethyl taurate copolymer, acrylamide / acryloyl dimethyl taurate copolymer, etc. At least one of sodium methyl taurate copolymer, xanthan gum, and carbomer; the emulsifier is selected from at least one of cetearyl glucoside, PEG-100 stearate, sucrose stearate, methyl glucosesquistearate, and cetearyl oleate / sorbitan oleate; the emollient is selected from at least one of caprylic / capric triglyceride, polydimethylsiloxane, cocoyl caprylic / capric ester, squalane, sweet almond oil, jojoba oil, isododecane, and diisopropyl dioleate.

[0018] Preferably, the skin care product is a cream, which, by weight percentage, comprises: 0.4-0.8% saffron extract, 0.01-0.03% Scutellaria baicalensis extract, 0.1-0.4% Edelweiss extract, 0.0005-0.005% Rhododendron simsii extract, 0.01-0.1% ergothioneine, 0.000002-0.00002% penetration enhancer, 0.01-0.04% chelating agent, 4-11% moisturizer, 1-4% thickener, 2-4% emulsifier, 2-10% skin moisturizer, 0.1-1% preservative, and the balance being water.

[0019] Through the above technical solution, a composition containing alpine plant extracts and ergothioneine can be prepared into a cream by adding excipients such as chelating agents, moisturizers, thickeners, emulsifiers, skin moisturizers, and preservatives. This can improve the stability of the product, enhance the moisturizing effect, improve the skin feel and texture, and enhance the safety of the product.

[0020] Preferably, the skincare product is in the form of a cream, and by weight percentage, the cream comprises: 0.4-0.8% saffron extract, 0.01-0.03% Scutellaria baicalensis extract, 0.1-0.4% Edelweiss extract, 0.0005-0.005% Rhododendron rose extract, 0.01-0.1% ergothioneine, 0.000001-0.00001% decapeptide-4, 0.000001-0.00001% glycoprotein, and 0.01-0.05% EDTA dimethyl ether. Sodium, 3-8% butanediol, 1-4% glycerol, 0.1-0.8% ammonium acryloyldimethyltaurate / VP copolymer, 1.2-1.8% cetearyl alcohol, 1.5-2.5% cetearyl alcohol or cetearyl glucoside, 1.2-1.8% PEG-100 stearate or glyceryl stearate, 3-7% caprylic / capric triglyceride, 1-3% polydimethylsiloxane, 3-7% caprylic / capric triglyceride, 1-3% polydimethylsiloxane, 0.5-1% preservative, and the balance being water.

[0021] Preferably, the skincare product is in the form of a serum, which, by mass percentage, comprises: 0.4-0.8% saffron extract, 0.01-0.03% Scutellaria baicalensis extract, 0.1-0.4% Edelweiss extract, 0.0005-0.005% Rhododendron simsii extract, 0.01-0.1% ergothioneine, 0.000002-0.00002% penetration enhancer, 0.01-0.04% chelating agent, 4-11% moisturizer, 0.1-0.5% thickener, 1-3% pH adjuster, 0.1-1% preservative, and the balance being water.

[0022] Using the above technical solution, a composition containing alpine plant extracts and ergothioneine is prepared into an essence after adding excipients such as chelating agents, moisturizers, thickeners, pH adjusters, and preservatives. This essence has excellent stability, moisturizing properties, and can improve skin feel and texture.

[0023] Preferably, the essence comprises, by weight percentage: 0.4-0.8% saffron extract, 0.01-0.03% Scutellaria baicalensis extract, 0.1-0.4% Edelweiss extract, 0.0005-0.005% Rhododendron simsii extract, 0.01-0.1% ergothioneine, 0.000001-0.00001% decapeptide-4, 0.000001-0.00001% glycoprotein, 0.01-0.04% disodium EDTA, 3-8% butylene glycol, 1-3% glycerin, 0.05-0.2% xanthan gum, 0.05-0.3% carbomer, 1-3% sodium hydroxide, 0.1-1% preservative, and the balance being water.

[0024] In summary, this application includes at least one of the following beneficial technical effects:

[0025] 1. This application provides a composition containing alpine plant extracts and ergothioneine. The composition includes saffron extract, alpine scutellaria extract, alpine edelweiss extract, alpine rose rhododendron extract, and ergothioneine. These ingredients work together through multiple pathways such as anti-oxidation, anti-inflammation, moisturizing, and promoting collagen synthesis. They can not only effectively resist the damage of the external environment to the skin, but also deeply nourish and repair the skin, thereby achieving better anti-photoaging, soothing, and antioxidant skin care effects.

[0026] 2. This application also provides a skin care product containing the above-mentioned composition of alpine plant extract and ergothioneine, which has excellent anti-photoaging, soothing and antioxidant skin care effects. Detailed Implementation

[0027] The present invention will be further described below with reference to specific embodiments. However, the following embodiments are for illustrative purposes only and should not be considered as limiting the scope of the invention. Unless otherwise specified, specific conditions in the following embodiments are performed under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the methods used are conventional methods known in the art, and the consumables and reagents used are commercially available. Unless otherwise stated, the technical and scientific terms used herein have the same meaning as those familiar with the art. Furthermore, any methods or materials similar to or equivalent to those described herein may also be applied to the present invention. Saffron extract was purchased from Jiangxi Yisenyuan Plant Fragrance Co., Ltd.; Alpine Scutellaria baicalensis extract, ALPAFLORSCUTELLARIA CB, was purchased from DSM Vitamins (Shanghai) Co., Ltd.; Alpine Edelweiss extract, Edelweiss extract C, was purchased from Guangzhou Luojie Biotechnology Co., Ltd.; Alpine Rhododendron flower extract, brand name Alpenrose flower (CH), was purchased from Guangzhou Chengzhi Trading Co., Ltd. Extract Glycerine unpreserved; ergothioneine purchased from Jiangsu Jinsan Biotechnology Co., Ltd.; decapeptide-4 purchased from Fujian Longsheng Biotechnology Co., Ltd.; glycoprotein purchased from Fujian Longsheng Biotechnology Co., Ltd.; ammonium acryloyl dimethyl taurate / VP copolymer, brand name: KLEASOL AVC, purchased from SNF SA; disodium EDTA purchased from: Noryon Chemicals (Ningbo) Co., Ltd.; cetearyl alcohol, brand name: RFA C1618, purchased from Global Green Chemicals Public Company Limited.

[0028] Example 1

[0029] A cream containing a composition of alpine plant extracts and ergothioneine, the composition of which is shown in Table 1 below.

[0030] Table 1

[0031]

[0032]

[0033] The method for preparing the cream containing the above-mentioned alpine plant extract and ergothioneine includes the following steps:

[0034] 1) Add deionized water to a mixing pot and heat to 78-82℃. Add humectant, chelating agent, thickener and preservative p-hydroxyacetophenone in sequence and homogenize for 3-5 minutes until uniform.

[0035] 2) Mix the emollient and emulsifier and heat to 78-82℃. Stir until completely melted, then add to a mixing pot and homogenize for 3-5 minutes until emulsification is complete.

[0036] 3) When the mixture obtained by homogenization in step 2) is cooled to 40°C, the composition containing alpine plant extract and ergothioneine and the preservative 1,2-hexanediol are added and stirred (10-30 rpm) until uniform to obtain a cream containing the composition containing alpine plant extract and ergothioneine.

[0037] Example 2

[0038] Example 2 provides a cream containing a composition of alpine plant extracts and ergothioneine. The only difference between Example 2 and Example 1 is that the mass percentages of saffron extract and edelweiss extract are different. In Example 2, the mass percentage of saffron extract is 0.45% and the mass percentage of edelweiss extract is 0.45%.

[0039] Example 3

[0040] Example 3 provides a cream containing a composition of alpine plant extracts and ergothioneine. The only difference between Example 3 and Example 1 is that the mass percentages of saffron extract and edelweiss extract are different. In Example 3, the mass percentage of saffron extract is 0.8% and the mass percentage of edelweiss extract is 0.1%.

[0041] Example 4

[0042] Example 4 provides a cream containing a composition of alpine plant extract and ergothioneine. The only difference between Example 4 and Example 1 is that the raw material components are different. The raw material components in Example 4 are shown in Table 2 below.

[0043] Table 2

[0044]

[0045]

[0046] Example 5

[0047] Example 5 provides a cream containing a composition of alpine plant extract and ergothioneine. The only difference between Example 5 and Example 1 is that the raw material components are different. The raw material components in Example 5 are shown in Table 3 below.

[0048] Table 3

[0049]

[0050]

[0051] Comparative Example 1

[0052] Comparative Example 1 provides a cream containing a composition of alpine plant extracts and ergothioneine. The only difference between Comparative Example 1 and Example 1 is that the saffron extract is replaced by edelweiss extract.

[0053] Comparative Example 2

[0054] Comparative Example 2 provides a cream containing a composition of alpine plant extracts and ergothioneine. The only difference between Comparative Example 2 and Example 1 is that the same amount of edelweiss extract is replaced with saffron extract.

[0055] Comparative Example 3

[0056] Comparative Example 3 provides a cream containing a composition of alpine plant extracts and ergothioneine. The only difference between Comparative Example 3 and Example 1 is that alpine rose rhododendron extract is not added.

[0057] Comparative Example 4

[0058] Comparative Example 4 provides a cream containing a composition of alpine plant extract and ergothioneine. The only difference between Comparative Example 4 and Example 1 is that alpine Scutellaria baicalensis extract is not added.

[0059] Comparative Example 5

[0060] Comparative Example 5 provides a cream containing a composition of alpine plant extracts. The only difference between Comparative Example 5 and Example 1 is that ergothioneine is not added.

[0061] Detection

[0062] 1) The photoaging effects of the creams containing alpine plant extracts and ergothioneine obtained in the examples and comparative examples were tested.

[0063] Establishment of UV-induced photoaging model cell culture: HaCaT cells were cultured in DMEM medium with 10% heat-inactivated fetal bovine serum (FBS) added, and cultured in an incubator at 37°C and 5% CO2.

[0064] UV radiation treatment: HaCaT cells in the logarithmic growth phase were selected and subjected to UVB radiation (80 mJ / cm²). 2 Cells were treated to establish a photoaging model.

[0065] The creams containing alpine plant extracts and ergothioneine obtained in the examples and comparative examples were added to the culture medium at a concentration of 10 mg / L and treated for 24 h. A blank control group (without UV radiation treatment and no cream added) and a UV group (without UV radiation treatment and no cream added) were also set up. Cell viability was measured, and the formula for calculating cell viability was: Cell viability = (Dexperimental group - Dblank control group) / (DUV group - Dblank control group), where Dexperimental group is the absorbance OD value of the experimental group at 450 nm, Dblank control group is the absorbance OD value of the blank control group at 450 nm, and DUV group is the absorbance OD value of the UV group at 450 nm. The results are shown in Table 4.

[0066] Table 4

[0067]

[0068]

[0069] As shown in Table 4, when comparing Comparative Examples 1 and 2 with Example 1, saffron extract and edelweiss extract exhibit a significant synergistic effect in improving the skin's anti-photoaging properties under this system.

[0070] As shown in Table 4, when Comparative Example 3 and Example 1 are not added, the anti-photoaging effect of the cream on the skin is significantly reduced.

[0071] As shown in Table 4, when Comparative Example 4 and Example 1 are not added, the anti-photoaging effect of the cream on the skin is significantly reduced.

[0072] As shown in Table 4, when Comparative Example 5 and Example 1 are compared, the anti-photoaging effect of the cream on the skin is significantly reduced when ergothioneine is not added.

[0073] As shown in Table 4, when comparing Examples 2 and 3 with Example 1, the weight ratio of saffron extract to edelweiss extract significantly affects the anti-photoaging effect of the cream on the skin. When the weight ratio of saffron extract to edelweiss extract is (1-8):1, the cream has the best anti-photoaging effect on the skin.

[0074] As shown in Table 4, when the composition containing alpine plant extracts and ergothioneine includes 0.4-0.8 parts by weight of saffron extract, 0.01-0.03 parts by weight of Scutellaria baicalensis extract, 0.1-0.4 parts by weight of Edelweiss extract, 0.0005-0.005 parts by weight of Rhododendron rose extract, and 0.01-0.1 parts by weight of ergothioneine, the cream prepared therefrom has a good anti-photoaging effect on the skin.

[0075] 2) The soothing effects of the creams containing alpine plant extracts and ergothioneine obtained in Examples 1-3 and Comparative Examples 1-5 were tested.

[0076] RAW264.7 cells were cultured in DMEM medium containing 10% FBS and placed in an incubator at 37°C and 5% CO2. RAW264.7 cells were stimulated with 200 ng / mL LPS. After LPS stimulation, TNF-α and IL-6 cytokines were significantly upregulated. Creams containing alpine plant extracts and ergothioneine obtained in Examples 1-3 and Comparative Examples 1-5 were added to the culture medium at 2.5 mg / L and treated for 24 h. A blank control group (no LPS stimulation and no cream added) and an LPS group (LPS stimulation and no cream added) were also set up. The levels of TNF-α and IL-6 were measured. The results are shown in Table 5.

[0077] Table 5

[0078]

[0079]

[0080] As shown in Table 5, when comparing Comparative Examples 1 and 2 with Example 1, saffron extract and edelweiss extract exhibit a significant synergistic effect in skin soothing under this system.

[0081] As shown in Table 5, when Comparative Example 3 and Example 1 are compared, the soothing effect of the cream on the skin is significantly reduced when alpine rose rhododendron extract is not added.

[0082] As shown in Table 5, when Comparative Example 4 and Example 1 are compared, the soothing effect of the cream on the skin is significantly reduced when Scutellaria baicalensis extract is not added.

[0083] As shown in Table 5, when Comparative Example 5 is compared with Example 1, the soothing effect of the cream on the skin is significantly reduced when ergothioneine is not added.

[0084] As shown in Table 5, when comparing Examples 2 and 3 with Example 1, the weight ratio of saffron extract to edelweiss extract significantly affects the soothing effect of the cream on the skin. The cream has the best soothing effect on the skin when the weight ratio of saffron extract to edelweiss extract is (1-8):1.

[0085] 3) In vitro DPPH free radical scavenging tests were conducted on the creams containing alpine plant extracts and ergothioneine obtained in Examples 1-3 and Comparative Examples 1-5. The creams containing alpine plant extracts and ergothioneine obtained in Examples 1-3 and Comparative Examples 1-5 were diluted with PBS to a concentration of 40 μg / mL to prepare test solutions. A 0.1 mmol / L DPPH ethanol solution was prepared. Table 6 shows the reaction system for the in vitro DPPH free radical scavenging test.

[0086] Table 6. Reaction system for in vitro DPPH free radical scavenging test

[0087]

[0088] The reaction system was placed at room temperature and reacted in the dark for 30 minutes. After the reaction was completed, the absorbance OD value was read at 517 nm, and the scavenging rate of the sample against DPPH free radicals was calculated according to the following formula.

[0089] In the formula, C1 is the absorbance OD value of the blank DPPH system at 517nm;

[0090] C2 is the absorbance OD value of the blank DPPH-free system at 517nm;

[0091] T1 is the absorbance OD value of the DPPH system in the sample group at 517 nm;

[0092] T2 is the absorbance OD value of the DPPH-free system in the sample group at 517 nm.

[0093] The test results of Examples 1-3 and Comparative Examples 1-5 are shown in Table 7.

[0094] Table 7

[0095] Example DPPH radical scavenging rate Example 1 56.9％ Example 2 65.1％ Example 3 63.9％ Comparative Example 1 22.3％ Comparative Example 2 26.2％ Comparative Example 3 38.1％ Comparative Example 4 35.9％ Comparative Example 5 17.9％

[0096] As shown in Table 7, when comparing Comparative Examples 1 and 2 with Example 1, it can be seen that, under this system, saffron extract and edelweiss extract exhibit a significant synergistic effect in terms of antioxidant properties.

[0097] As shown in Table 7, when Comparative Example 3 and Example 1 are not added, the antioxidant effect of the cream on the skin is significantly reduced.

[0098] As shown in Table 7, when Comparative Example 4 and Example 1 are compared, the antioxidant effect of the cream on the skin is significantly reduced when Scutellaria baicalensis extract is not added.

[0099] As shown in Table 7, when Comparative Example 5 and Example 1 are not added, the antioxidant effect of the cream on the skin is significantly reduced.

[0100] As shown in Table 7, when comparing Examples 2 and 3 with Example 1, the weight ratio of saffron extract to edelweiss extract significantly affects the antioxidant effect of the cream on the skin. The cream has the best antioxidant effect on the skin when the weight ratio of saffron extract to edelweiss extract is (1-8):1.

[0101] Example 6

[0102] The embodiment provides an essence containing a composition of alpine plant extracts and ergothioneine, the composition of which is shown in Table 8 below.

[0103] Table 8

[0104]

[0105] A method for preparing an essence containing a composition of alpine plant extracts and ergothioneine, comprising the following steps:

[0106] 1) Add deionized water to a mixing bowl and heat to 80-85℃. Add the humectant, chelating agent, thickener, and preservative p-hydroxyacetophenone in sequence, stirring until completely dissolved.

[0107] 2) Homogenize the mixture obtained in step 1), cool it to 40°C, and then add the pH adjuster, the preservative 1,2-hexanediol, and the composition containing alpine plant extract and ergothioneine in sequence. Stir well to obtain an essence containing the composition of alpine plant extract and ergothioneine.

[0108] Comparative Example 6

[0109] Comparative Example 6 provides an essence containing a composition of alpine plant extracts and ergothioneine. The only difference between Comparative Example 6 and Example 6 is that the saffron extract is replaced by edelweiss extract.

[0110] Comparative Example 7

[0111] Comparative Example 7 provides an essence containing a composition of alpine plant extracts and ergothioneine. The only difference between Comparative Example 7 and Example 6 is that the same amount of edelweiss extract is replaced with saffron extract.

[0112] Detection

[0113] 4) The photoaging effects of the serums containing alpine plant extracts and ergothioneine obtained in Example 6 and Comparative Examples 6 and 7 were tested.

[0114] Establishment of UV-induced photoaging model cell culture: HaCaT cells were cultured in DMEM medium with 10% heat-inactivated fetal bovine serum (FBS) added, and cultured in an incubator at 37°C and 5% CO2.

[0115] UV radiation treatment: HaCaT cells in the logarithmic growth phase were selected and subjected to UVB radiation (dose 80 mJ / cm²). 2 Cells were treated to establish a photoaging model.

[0116] The extracts of the compositions containing alpine plant extracts and ergothioneine obtained in Examples 6 and 6 and 7 were added to the culture medium at a concentration of 10 mg / L and treated for 24 h. A blank control group (without UV radiation treatment and no extract added) and a UV group (without UV radiation treatment and no extract added) were also set up. Cell viability was measured. The formula for calculating cell viability was: Cell viability = (Dexperimental group - Dblank control group) / (DUV group - Dblank control group), where Dexperimental group is the absorbance OD value of the experimental group at 450 nm, Dblank control group is the absorbance OD value of the blank control group at 450 nm, and DUV group is the absorbance OD value of the UV group at 450 nm. The results are shown in Table 9.

[0117] Table 9

[0118] Example Cell vitality Example 6 151.53％ UV group 100％ Blank control group 206.52％ Comparative Example 6 117.53％ Comparative Example 7 116.96％

[0119] As shown in Table 9, when comparing Comparative Examples 6 and 7 with Example 6, saffron extract and edelweiss extract exhibit a significant synergistic effect in improving the skin's anti-photoaging properties under this system.

[0120] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A composition containing alpine plant extract and ergothioneine, characterized in that, include: 0.005-0.8 parts by weight of saffron extract, 0.01-0.03 parts by weight of Scutellaria baicalensis extract, 0.1-0.4 parts by weight of Edelweiss extract, 0.0005-0.005 parts by weight of Rhododendron rose extract, and 0.001-0.15 parts by weight of ergothioneine; wherein the weight ratio of the saffron extract to the Edelweiss extract is (1-8):

1.

2. The composition containing alpine plant extract and ergothioneine according to claim 1, characterized in that, include: 0.4-0.8 parts by weight of saffron extract, 0.01-0.03 parts by weight of Scutellaria baicalensis extract, 0.1-0.4 parts by weight of Edelweiss extract, 0.0005-0.005 parts by weight of Rhododendron rose extract, and 0.01-0.1 parts by weight of ergothioneine.

3. The composition containing alpine plant extract and ergothioneine according to claim 1, characterized in that, The composition containing alpine plant extracts and ergothioneine further includes a penetration enhancer comprising 0.000001-0.00001 parts by weight of decapeptide-4 and 0.000001-0.00001 parts by weight of glycoprotein.

4. The use of the composition containing alpine plant extract and ergothioneine as described in any one of claims 1-3 in the preparation of anti-photoaging, soothing, and whitening cosmetics or skin care products.

5. A skincare product, characterized in that, The skin care product includes the composition containing alpine plant extract and ergothioneine as described in any one of claims 1-3, wherein the skin care product is any one of liquid, lotion, cream, oil or powder.

6. The skincare product according to claim 5, characterized in that, The composition containing alpine plant extracts and ergothioneine is present in the skincare product at a mass percentage of 0.9405-1.0116%.

7. The skincare product according to claim 5, characterized in that, The skincare product further comprises at least one of a chelating agent, a moisturizer, a thickener, an emulsifier, a skin moisturizer, a preservative, a solvent, and a pH adjuster. The chelating agent is disodium EDTA or tetrasodium EDTA. The moisturizer is selected from at least one of butylene glycol, glycerin, propylene glycol, and vegetable oil. The thickener is selected from at least one of ammonium acryloyl dimethyl taurate / VP copolymer, sodium polyacryloyl dimethyl taurate, sodium acrylate / sodium acryloyl dimethyl taurate copolymer, acrylamide / sodium acryloyl dimethyl taurate copolymer, xanthan gum, and carbomer. The emulsifier is selected from at least one of cetearyl glucoside, PEG-100 stearate, sucrose stearate, methyl glucosesquistearate, and cetearyl oleate / sorbitan oleate. The skin moisturizer is selected from at least one of caprylic / capric triglyceride, polydimethylsiloxane, cocoyl caprylic / capric ester, squalane, sweet almond oil, jojoba oil, isododecane, and diisopropyl dioleate.

8. The skincare product according to claim 5, characterized in that, The skincare product is a cream, which, by weight percentage, comprises: 0.4-0.8% saffron extract, 0.01-0.03% Scutellaria baicalensis extract, 0.1-0.4% Edelweiss extract, 0.0005-0.005% Rhododendron simsii extract, 0.01-0.1% ergothioneine, 0.000002-0.00002% penetration enhancer, 0.01-0.04% chelating agent, 4-11% moisturizer, 1-4% thickener, 2-4% emulsifier, 2-10% skin moisturizer, 0.1-1% preservative, and the balance being water.

9. The skincare product according to claim 5, characterized in that, The skincare product is an essence, which, by weight percentage, comprises: 0.4-0.8% saffron extract, 0.01-0.03% Scutellaria baicalensis extract, 0.1-0.4% Edelweiss extract, 0.0005-0.005% Rhododendron simsii extract, 0.01-0.1% ergothioneine, 0.000002-0.00002% penetration enhancer, 0.01-0.04% chelating agent, 4-11% moisturizer, 0.1-0.5% thickener, 1-3% pH adjuster, 0.1-1% preservative, and the balance being water.

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