Traditional Chinese medicine essential oil for treating lung and kidney deficiency type stress obesity and preparation method thereof

Abstract

The invention provides traditional Chinese medicine essential oil for treating lung and kidney deficiency type stress obesity and a preparation method of the traditional Chinese medicine essential oil. The traditional Chinese medicine essential oil comprises a traditional Chinese medicine composition, and active ingredients of the traditional Chinese medicine composition are prepared from, by weight, 15-45 parts of rhodiola rosea, 15-45 parts of saussurea involucrata, 5-15 parts of cinnamon, 5-15 parts of sandalwood and 10-30 parts of Tibet inula roots. The active components of the traditional Chinese medicine composition cooperate with each other and supplement each other, and the traditional Chinese medicine composition has multiple effects of tonifying the lung and kidney, promoting blood circulation to remove blood stasis, calming the heart and tranquilizing the mind and the like, can relieve the symptom of pressure obesity caused by deficiency of the lung and the kidney from multiple aspects, and achieves the effect of overall regulation.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine essential oil for treating stress obesity of the lung and kidney deficiency type and a preparation method thereof. Background Art

[0002] Stress obesity is a metabolic disease caused by long-term psychological or physiological stress. In today's society, the incidence of stress obesity is increasing year by year, which is closely related to the high work pressure, fast pace of life and unhealthy lifestyle in modern society. Research shows that the obesity risk of people who have been in a high-stress environment for a long time is about 30%-40% higher than that of the general population. And obesity, as the basis of metabolic diseases such as fatty liver, diabetes, hypertension and cardiovascular and cerebrovascular diseases, will pose a severe challenge to the social public health environment and cause a huge economic burden.

[0003] Since stress is one of the fundamental causes of stress obesity, effective stress reduction methods are the key to treating this disease. Currently, the main stress reduction strategies include cognitive behavioral therapy, meditation and relaxation training, etc. At the same time, behavioral interventions including diet and exercise intervention and emotion management are also the main treatment options for stress obesity. In some cases, such as when stress obesity is accompanied by severe weight gain or other health problems (such as diabetes, hypertension), weight loss drugs, insulin sensitizers, cortisol antagonists and antidepressants should be selected.

[0004] However, it is worth noting that although the intervention strategies for stress obesity have been developing rapidly in recent years, there is still no satisfactory treatment drug so far. Therefore, there is still a huge technical gap at present, and the research and development of related treatment drugs has always been a research hotspot pursued by the international academic community and pharmaceutical companies. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to provide a traditional Chinese medicine essential oil for treating stress obesity of the lung and kidney deficiency type and a preparation method thereof.

[0006] To achieve the above purpose, the present invention adopts the following technical solutions.

[0007] The first aspect of the present invention provides a traditional Chinese medicine composition for treating stress obesity, and the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 15-45 parts of Rhodiola rosea, 15-45 parts of Saussurea involucrata, 5-15 parts of Cinnamomum cassia, 5-15 parts of Santalum album, 10-30 parts of Inula racemosa.

[0008] In some embodiments, the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 20-40 parts of Rhodiola rosea, 20-40 parts of Saussurea involucrata, 6-12 parts of Cinnamomum cassia, 6-12 parts of Santalum album, 15-25 parts of Inula racemosa.

[0009] In some embodiments, the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 28-32 parts of Rhodiola rosea, 28-32 parts of Saussurea involucrata, 8-12 parts of Cinnamon, 8-12 parts of Santalum album, and 18-22 parts of Inula racemosa.

[0010] The second aspect of the present invention provides a preparation method of the traditional Chinese medicine composition as described above, comprising the following steps: taking each raw material according to the dosage, adding ethanol for extraction 1-3 times; combining the filtrates, removing the solvent, to obtain the traditional Chinese medicine composition.

[0011] In some embodiments, the temperature of the extraction is 50°C - 60°C, and the time is 1.5 h - 4 h.

[0012] In some embodiments, the volume fraction of the ethanol is 85% - 100%.

[0013] In some embodiments, the ratio of the total weight of the raw materials to the ethanol is 1:(5 - 10) g / mL.

[0014] In some embodiments, the method for removing the solvent is vacuum concentration.

[0015] In some embodiments, the relative density of the traditional Chinese medicine composition is 0.90 - 1.05, preferably 0.95 - 1.00.

[0016] The third aspect of the present invention provides the application of the traditional Chinese medicine composition as described above in the preparation of a drug for treating stress obesity.

[0017] In some embodiments, the stress obesity is stress obesity of the deficiency type of the lung and kidney.

[0018] The fourth aspect of the present invention provides a drug for treating stress obesity, and the active ingredients of the drug comprise the traditional Chinese medicine composition as described above.

[0019] In some embodiments, the dosage form of the drug is an external dosage form, preferably essential oil.

[0020] In some embodiments, the essential oil is a base oil containing the traditional Chinese medicine composition, and the volume fraction of the traditional Chinese medicine composition in the essential oil is 1% - 5%, preferably 2% - 5%.

[0021] In some embodiments, the base oil includes at least one of sweet almond oil, safflower oil, coconut oil, linseed oil, and grape seed oil.

[0022] In some embodiments, the preparation method of the drug comprises the following steps: adding the traditional Chinese medicine composition into the base oil, and mixing evenly to obtain the drug.

[0023] The present invention has obtained a traditional Chinese medicine composition for treating stress obesity of the lung and kidney deficiency type through research. The active ingredients of the traditional Chinese medicine composition are prepared from raw materials of Rhodiola rosea, Saussurea involucrata, Cinnamomum cassia, Santalum album, and Inula racemosa in appropriate weight parts. In the formula, Rhodiola rosea replenishes qi and activates blood circulation, tonifies the lung and kidney, and is the monarch drug; Saussurea involucrata promotes blood circulation to remove blood stasis and tonifies the kidney and regulates menstruation, and Santalum album soothes the nerves and opens orifices to relieve depression. The two are used together as ministerial drugs; Cinnamomum cassia promotes blood circulation to remove blood stasis and warms the middle-jiao to dispel cold, and is the assistant drug; Inula racemosa promotes qi and strengthens the spleen, and is the guiding drug. The active ingredients of the volatile substances extracted from the five raw materials cooperate with each other, complement each other, and have multiple effects such as tonifying the lung and kidney, promoting blood circulation to remove blood stasis, and soothing the nerves. They can relieve the symptoms of stress obesity of the lung and kidney deficiency type from multiple aspects and achieve the effect of overall regulation.

[0024] The drug containing the traditional Chinese medicine composition of the present invention, such as essential oil, has the advantages of convenient use and no side effects, can be better applied to the prevention and treatment intervention of stress obesity, can be used as a representative of traditional Chinese medicine aromatherapy for a long time, and has good prospects for achievement transformation. Description of the Drawings

[0025] Figure 1 It is the detection result of the body weight of each group of mice.

[0026] Figure 2 It is the detection result of the food intake of each group of mice.

[0027] Figure 3 It is the detection result of the contents of TG and TC in the serum of each group of mice.

[0028] Figure 4 It is the detection result of the blood glucose of each group of mice.

[0029] Figure 5 It is the detection result of the HE and Oil Red O staining of the liver of each group of mice.

[0030] Figure 6 It is the detection result of the contents of GABA in the hypothalamus and serum of each group of mice. Detailed Description of the Invention

[0031] In the following examples of the present invention, the experimental methods without specific conditions are usually carried out under conventional conditions or according to the conditions recommended by the manufacturers. All kinds of commonly used chemical reagents used in the examples are commercially available products.

[0032] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the description of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0033] The terms "comprising" and "having" and any variations thereof in the present invention are intended to cover non-exclusive inclusion. For example, a process, method, device, product or equipment that includes a series of steps is not limited to the listed steps or modules, but may optionally further include steps not listed, or may optionally further include other steps inherent to these processes, methods, products or equipment.

[0034] As used in the present invention, "a plurality of" means two or more. "And / or" describes the association relationship of associated objects and indicates that three relationships may exist. For example, A and / or B may represent three cases: A exists alone, A and B exist simultaneously, and B exists alone.

[0035] In some embodiments of the present invention, there is provided a traditional Chinese medicine composition for treating stress obesity. The active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 15-45 parts of Rhodiola rosea, 15-45 parts of Saussurea involucrata, 5-15 parts of Cinnamomum cassia, 5-15 parts of Santalum album, and 10-30 parts of Inula racemosa.

[0036] From the perspective of traditional Chinese medicine, stress-induced obesity is mostly related to liver qi stagnation, disharmony between the spleen and stomach, deficiency of the lungs and kidneys, phlegm coagulation and blood stasis, and restlessness of the mind. Among them, the heart governs the mind, and restlessness of the mind affects sleep quality and emotional stability, making it difficult to relieve stress; the liver is responsible for dredging and regulating qi, and emotional stress can lead to stagnation of liver qi, poor qi movement, and affect the function of the spleen and stomach; the spleen is the foundation of acquired constitution, and disharmony of the spleen and stomach functions and abnormal transportation may lead to increased appetite and obesity; the kidney is the foundation of congenital constitution, the lungs and kidneys regulate qi movement and water metabolism up and down, and deficiency of the lungs and kidneys is likely to lead to phlegm coagulation and blood stasis, and qi stagnation. The inventor of the present invention combined long-term clinical practice experience and a large number of studies, and proposed that deficiency of the lungs and kidneys is a common clinical syndrome type of stress-induced obesity. At the same time, it is pointed out that the above-mentioned etiological factors and pathogenesis will affect each other during the disease progression process, and comprehensive consideration is needed in prevention and treatment interventions. A traditional Chinese medicine composition for treating stress-induced obesity of the deficiency type of the lungs and kidneys is proposed. The active ingredients of the traditional Chinese medicine composition are prepared from suitable parts by weight of Rhodiola rosea, Saussurea involucrata, Cinnamomum cassia, Santalum album, and Inula racemosa. In the formula, Rhodiola rosea is used as the monarch drug. Rhodiola rosea has the functions of replenishing qi and promoting blood circulation, and tonifying the lungs and kidneys, and can provide the main treatment direction for the whole prescription, targeting the symptoms of stress-induced obesity and deficiency of the lungs and kidneys. Saussurea involucrata and Santalum album are used as ministerial drugs together. Saussurea involucrata promotes blood circulation to remove blood stasis and tonifies the kidney and regulates menstruation, enhancing the tonifying effect of Rhodiola rosea, helping to improve blood circulation and regulate kidney function; Santalum album has the functions of calming the mind and opening the orifices and relieving depression, and can relieve anxiety and depression caused by stress, help regulate emotions, and promote overall physical and mental health. Cinnamomum cassia is used as an adjuvant drug. Cinnamomum cassia promotes blood circulation to remove blood stasis and warms the middle-jiao to dispel cold, and can enhance the overall yang-warming effect, help improve the function of the spleen and stomach, and promote qi and blood circulation. Inula racemosa is used as a guiding drug. Inula racemosa promotes qi and strengthens the spleen, can guide the effects of other drugs, promote the circulation of qi and blood, enhance the effect of the whole prescription, help improve the function of the spleen and stomach, and promote the exertion of drug effects. The active ingredients of the volatile substances extracted from the five raw materials complement each other and have multiple effects such as tonifying the lungs and kidneys, promoting blood circulation to remove blood stasis, and calming the mind, and can relieve the symptoms of stress-induced obesity of the deficiency type of the lungs and kidneys from multiple aspects, achieving the effect of overall regulation.

[0037] The volatile substances extracted from the five raw materials of the traditional Chinese medicine composition of the present invention can regulate the content of neurotransmitters such as gamma-aminobutyric acid (GABA) in the brain, and then participate in the regulation process of various physiological functions such as emotion, sleep, and eating, and can effectively reduce the body weight of stress-induced obesity model mice.

[0038] In some embodiments of the present invention, a preparation method of the above-mentioned traditional Chinese medicine composition is involved, including the following steps: taking each raw material according to the dosage, adding ethanol for extraction 1 to 3 times; combining the filtrates, removing the solvent, to obtain the traditional Chinese medicine composition.

[0039] In some embodiments, the temperature of the extraction is 50°C to 60°C, and the time is 1.5 h to 4 h.

[0040] In some embodiments, the volume fraction of the ethanol is 85% - 100%.

[0041] In some embodiments, the liquid ratio of the total weight of the raw materials to the amount of ethanol is 1:(5 - 10) g / mL.

[0042] In some embodiments, the method for removing the solvent is the reduced pressure concentration method.

[0043] In some embodiments, the conditions of the reduced pressure concentration method are as follows: the pressure is 20 mmHg - 50 mmHg, the heating temperature is 40°C - 60°C, the rotation speed is 150 rpm - 200 rpm, and the evaporation time is 0.5 h - 2 h.

[0044] In some embodiments, the conditions of the reduced pressure concentration method are as follows: the pressure is 25 mmHg - 30 mmHg, the heating temperature is 45°C - 55°C, the rotation speed is 130 rpm - 180 rpm, and the evaporation time is 1 h - 1.5 h.

[0045] In some embodiments, the relative density of the traditional Chinese medicine composition is 0.90 - 1.05, preferably 0.95 - 1.00.

[0046] In some embodiments of the present invention, there is provided a drug for treating stress obesity, and the active ingredient of the drug comprises the traditional Chinese medicine composition as described above.

[0047] The traditional Chinese medicine composition of the present invention can be made into a traditional Chinese medicine preparation for treating stress obesity together with conventional excipients in the art, preferably an external preparation. For example, an appropriate amount of the traditional Chinese medicine composition is added to a base oil to make an essential oil for treating stress obesity, including an external application essential oil, an aromatherapy essential oil, etc.

[0048] In some embodiments, the volume fraction of the traditional Chinese medicine composition in the essential oil is 1% - 5%, including but not limited to 1%, 2%, 3%, 4%, 5%, or the range composed of any two of them.

[0049] In some embodiments, the base oil is at least one of sweet almond oil, safflower oil, coconut oil, linseed oil, and grape seed oil.

[0050] The following is illustrated with specific embodiments.

[0051] Example 1

[0052] This example provides a traditional Chinese medicine composition for treating stress obesity, and the active ingredient of the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30 parts of Rhodiola rosea, 30 parts of Saussurea involucrata, 10 parts of Cinnamomum cassia, 10 parts of Santalum album, and 20 parts of Inula racemosa.

[0053] The traditional Chinese medicine composition is prepared by the following method: Weigh rhodiola, snow lotus, cinnamon, sandalwood, and incarvillea root according to parts by weight. Use a mortar and pestle to grind the samples into fine powders to increase the extraction efficiency. Place the ground plant powders in a glass beaker and add 5 times the volume of 95% ethanol (volume fraction) of the total weight of the raw materials to completely cover the samples. Heat in a water bath to 55°C and stir for extraction for about 2.5 hours. Filter the extract using filter paper and a glass funnel to remove solid residues, and collect the filtrate in a clean beaker. Transfer the filtrate to a rotary evaporator and evaporate the solvent under reduced pressure as follows: pressure 30 mmHg, heating temperature at 50°C, rotation speed at 150 rpm, evaporate for about 1 - 1.5 hours until a concentrated extract is obtained, which has an obvious aroma and color, relative density 0.95, refractive index 1.48, thus obtaining the traditional Chinese medicine composition.

[0054] Example 2

[0055] In this example, taking the traditional Chinese medicine composition in Example 1 as an example, the therapeutic effect of the traditional Chinese medicine composition of the present invention on stress-induced obesity was studied.

[0056] 1. Experimental method

[0057] Using sweet almond oil as the base oil, add the traditional Chinese medicine composition described in Example 1 to sweet almond oil according to the volume fractions of the traditional Chinese medicine composition being 1%, 2%, and 3% respectively, and mix evenly to obtain essential oils with the volume fractions of the traditional Chinese medicine composition being 1%, 2%, and 3% respectively, for the study of the therapeutic effect on stress-induced obesity.

[0058] Use a high-fat, high-cholesterol, high-fructose diet, a restricted stress environment, and smoke stimulation to construct a mouse model of stress-induced obesity with deficiency of the lung and kidney. Set up a normal control group, a negative control group, and a drug essential oil treatment group respectively. Specifically as follows:

[0059] Select male C57BL / 6 mice at 6 - 8 weeks old. After one week of adaptive feeding, start the experiment. The mice are given a high-fat, high-cholesterol, high-fructose diet (XT310, Synergy Bio), given in sufficient quantity, allowed to eat freely without restricting the food intake, and the mice are randomly divided into 7 groups, with 6 mice in each group, and are treated as follows:

[0060] Group 1: Apply about 1 drop of the base oil to the back at an appropriate time (synchronized with the subsequent groups) as a normal control.

[0061] Group 2: Give a restricted stress environment (randomly select three days a week to place the mice in a mouse holder for 30 minutes) and smoke stimulation (place the mice receiving restricted stress stimulation in a glass hood and create smoke stimulation in the glass hood for 30 minutes), and apply about 1 drop of the base oil to the back after each restricted stress and smoke stimulation environment, as a negative control.

[0062] The group treated with medicinal essential oil included groups 3 to 7. Each group of mice was given the same restricted stress environment and smoke stimulation as in group 2, and about 1 drop of medicinal essential oil was applied to the back each time after the restricted stress and smoke stimulation environment was given. The medicinal essential oils used in groups 3 to 7 were as follows:

[0063] Group 3: the above-mentioned medicinal essential oil with a volume concentration of 1%.

[0064] Group 4: the above-mentioned medicinal essential oil with a volume concentration of 2% was applied.

[0065] Group 5: the above-mentioned medicinal essential oil with a volume concentration of 3% was applied.

[0066] Group 6: a control medicinal essential oil with a volume concentration of 3%. Except that the saussurea involucrata in the original formula was replaced with safflower with similar efficacy in equal amount in the traditional Chinese medicine composition of the active ingredient of the control medicinal essential oil, the others were the same as in Example 1. The control traditional Chinese medicine composition was obtained by the same method as in Example 1, and then it was added to sweet almond oil according to the volume fraction of 3% of the control traditional Chinese medicine composition and mixed evenly to obtain the control medicinal essential oil.

[0067] Group 7: a control medicinal essential oil with a volume concentration of 3%. Except that the incarvillea sinensis in the original formula was replaced with cyperus rotundus with similar efficacy in equal amount in the traditional Chinese medicine composition of the active ingredient of the control medicinal essential oil, the others were the same as in Example 1. The control traditional Chinese medicine composition was obtained by the same method as in Example 1, and then it was added to sweet almond oil according to the volume fraction of 3% of the control traditional Chinese medicine composition and mixed evenly to obtain the control medicinal essential oil.

[0068] Each group of mice was allowed to eat and drink freely and was raised for 12 weeks. The mental state and activity of the mice were observed regularly, and the body weight and food intake were recorded weekly; at the end of the experiment, the body weight and food intake of each group of mice were recorded, the blood glucose (FBG), triglyceride (TG), total cholesterol (TC) and GABA content were detected, and the liver tissue of the mice was stained with hematoxylin-eosin (HE) and oil red O. Among them, the body weight and food intake of the mice were directly recorded, the blood glucose was directly detected by a blood glucose meter, and TG and TC were directly detected by an automatic biochemical analyzer.

[0069] The detection method of GABA content was as follows: the serum and brain tissue of the mice were detected by enzyme-linked immunosorbent assay (ELISA, BY-JZF0215, the 13th section). The samples were treated with pre-cooled PBS or special lysis buffer, and the protein interference was removed by centrifugal ultrafiltration or acetonitrile precipitation method; the ELISA kit coated with specific GABA antibody was used for detection, and the samples were incubated, developed color according to the instructions, and the absorbance at 450 nm was measured. A standard curve was made synchronously, and the operation was carried out on ice throughout to prevent degradation. Each group was set with 6 replicates to ensure the accuracy of the data.

[0070] The HE staining method is as follows: Take out the liver tissue sample from the formalin fixative and continuously rinse it with running water for 4 h to remove the residual fixative and impurities. Subsequently, place the tissue sample in an automatic dehydrator for dehydration. After dehydration is completed, perform paraffin embedding in an embedding machine to make a paraffin block. Use a microtome to cut the paraffin block into sections with a thickness of 5 μm, and attach the sections to glass slides. Immerse the dried sections in xylene for 10 min successively and repeat once to completely remove the paraffin. Then immerse them in absolute ethanol for 5 min successively, repeat the immersion for 2 min again, and then immerse them in 95%, 90%, 80%, and 70% ethanol for 2 min each. Finally, rinse with distilled water and then immerse in PBS solution 3 times, 5 min each time, to ensure that the tissue is fully hydrated and impurities are removed. Immerse the sections in hematoxylin stain for 1 min to stain the cell nuclei. Rinse the sections with distilled water to remove the excess stain. Then place the sections in 0.4% hydrochloric acid alcohol for differentiation for 4 s, rinse the sections with distilled water again, and finally place the sections in 0.6% ammonia water for bluing treatment to make the cell nuclei stain more clearly. Finally, rinse the sections with running water. Immerse the sections in eosin stain for 2 min to stain the cytoplasm. Immerse the stained sections in 95% ethanol for 5 min successively, then in absolute ethanol for 5 min, and finally in xylene for 5 min to completely dehydrate the sections. After the sections are air-dried, drop neutral resin for coverslipping and take pictures.

[0071] The Oil Red O staining method is as follows: Pre-cool the frozen section machine in advance to ensure that the machine temperature is stable at an appropriate low temperature. Take out the frozen tissue sample, and after the sample temperature is balanced, place it on ice to melt for subsequent sectioning operations. Adhere the melted tissue sample to the frozen section stage with OCT glue to ensure that the tissue sample is firmly fixed. Place the stage with the adhered sample on the pre-cooled frozen section machine. After the OCT glue freezes and solidifies, section at a thickness of 8 μm. Gently adhere the sections with pre-labeled glass slides to ensure that the sections are firmly attached to the glass slides. Take out the frozen sections and rinse them in a container containing 60% isopropanol for 20 s to remove the impurities and residual OCT glue on the section surface. Mix Oil Red O dye and diluent in a ratio of 5:2, and filter it twice with filter paper to ensure that the staining solution is uniform and free of impurities. Immerse the sections in Oil Red O staining solution for 10 min to fully stain the lipid droplets. After staining, rinse the sections with 60% isopropanol for 20 s to remove the excess Oil Red O staining solution, and then wash the sections with pre-warmed double-distilled water at 37 °C to ensure that the section surface is clean. Use hematoxylin (counterstain) to counterstain the sections for 4 min to stain the cell nuclei. Perform coverslipping with aqueous mounting medium pre-warmed to liquid state at 60 °C and take pictures.

[0072] The statistical analysis method is as follows: GraphPad Prism 7.0 software was used to process the data, and the data were expressed as mean ± standard deviation. One-way ANOVA was used for inter-group comparison of indicators, and ANOVA for repeated measures was used for comparison of repeated measures data. P < 0.05 indicated significant difference.

[0073] 2. Experimental results

[0074] (1) Body weight and general condition of mice

[0075] As Figure 1 shown, it can be seen that the body weights of mice in all groups increased rapidly. Compared with the mice in group 1, the body weight growth rate of the mice in group 2 was significantly faster, and the difference between the two groups was statistically significant (P < 0.05), indicating that the stress and smoke stimulation environment led to a further significant increase in the body weight of mice. Activity restriction is a common modeling protocol for stress, and smoke stimulation can induce panic in mice and respiratory obstruction, which is a common modeling protocol for lung and kidney deficiency. At the same time, combined with the general condition, it was observed that the mice in group 2 showed phenomena such as being easily panicked and having obvious touch stress responses. Combining with the body weight, it can be considered that the mouse model of stress-induced obesity with lung and kidney deficiency was successfully established.

[0076] Compared with group 2, the body weight growth rates of the mice in groups 3, 4, 5, 6, and 7 were relatively slowed down. Among them, the differences between groups 4, 5 and group 2 were statistically significant (P < 0.05), indicating that the medicinal essential oil of the present invention can effectively reduce the body weight of stress-induced obesity mice with lung and kidney deficiency.

[0077] Group 5 was treated with the essential oil of the present invention. In group 6, the snow lotus in the active ingredient traditional Chinese medicine composition of the treatment essential oil was replaced with safflower with similar efficacy in equal amount. In group 7, the costus root in the active ingredient traditional Chinese medicine composition of the treatment essential oil was replaced with cyperus rotundus with similar efficacy in equal amount. From Figure 1 this, it can be seen that compared with group 5, the body weight growth rates of groups 6 and 7 were relatively faster, and the differences were statistically significant (P < 0.05). It shows that replacing the raw materials in the traditional Chinese medicine composition of the present invention will significantly affect the effect of the prepared traditional Chinese medicine composition on stress-induced obesity with lung and kidney deficiency, and it is impossible to ensure that the ideal medicinal effect can be achieved. The formula compatibility of the traditional Chinese medicine composition of the present invention is reasonable. The active ingredients of the volatile substances extracted from the five flavors of rhodiola rosea, snow lotus, cinnamon, sandalwood and costus root cooperate with each other, complement each other, and have multiple effects such as tonifying the lung and kidney, promoting blood circulation and removing blood stasis, and calming the mind. It can relieve the symptoms of stress-induced obesity with lung and kidney deficiency from multiple aspects and achieve the effect of overall regulation.

[0078] (2) Food intake of mice

[0079] As Figure 2As shown, it can be seen that the food intake of mice in all embodiments increased slowly synchronously with body weight. Compared with the mice in Group 1, the growth rate of food intake of the mice in Group 2 was significantly faster, and the difference between the two groups was statistically significant (P<0.05), indicating that the stress and smoke-stimulated environment led to an increase in the food intake of mice, which is also a typical manifestation of stress-induced obesity. Compared with Group 2, the growth rate of food intake of the mice in Groups 3, 4, 5, 6, and 7 all slowed down relatively. Among them, the differences between Groups 4 and 5 and Group 2 were statistically significant (P<0.05), indicating that the medicinal essential oil of the present invention can effectively reduce stress-induced eating, suggesting that it may be the potential pharmacological mechanism of the traditional Chinese medicine essential oil of the present invention in treating stress-induced obesity of the deficiency of the lung and kidney. At the same time, compared with Group 5, the growth rate of food intake of Groups 6 and 7 was relatively faster, and the difference was statistically significant (P<0.05).

[0080] (3) Blood lipids of mice

[0081] As Figure 3 shown, compared with Group 1, the contents of serum triglyceride (TG) and total cholesterol (TC) of the mice in Group 2 increased, and the difference was statistically significant (P<0.05). Compared with Group 2, the contents of serum TG and TC of the mice in Groups 3, 4, 5, 6, and 7 all decreased. Among them, the differences between Groups 4 and 5 and Group 2 were statistically significant (P<0.05), indicating that the medicinal essential oil of the present invention has a certain effect on improving the blood lipids of mice with stress-induced obesity of the deficiency of the lung and kidney, and assisting in the treatment of stress-induced obesity. At the same time, compared with Group 5, the blood lipid contents of Groups 6 and 7 increased relatively, and the difference between Group 7 and Group 5 was statistically significant (P<0.05).

[0082] (4) Blood glucose of mice

[0083] As Figure 4 shown, compared with Group 1, the content of blood glucose (FBG) of the mice in Group 2 increased, and the difference was statistically significant (P<0.05). Compared with Group 2, the serum blood glucose contents of the mice in Groups 3, 4, 5, 6, and 7 decreased. Among them, the differences between Groups 4 and 5 and Group 2 were statistically significant (P<0.05), indicating that the medicinal essential oil of the present invention has a certain effect on improving the blood glucose of mice with stress-induced obesity of the deficiency of the lung and kidney, and assisting in the treatment of stress-induced obesity. At the same time, compared with Group 5, the blood glucose of the mice in Groups 6 and 7 increased relatively.

[0084] (5) Hepatic steatosis and inflammatory response of mice

[0085] As Figure 5As shown, obvious lipid droplet accumulation and inflammatory reactions were observed in the liver tissues of each group of mice. Compared with group 1, fatty degeneration and inflammatory reactions were more obvious in the liver tissues of group 2 mice. Compared with group 2, the fatty degeneration and inflammatory reactions in the liver tissues of group 3, group 4, group 5, group 6, and group 7 mice were all improved. Among them, the improvement in group 5 was the most obvious, indicating that the medicinal essential oil of the present invention has a certain effect in improving fatty liver in mice with stress obesity of lung and kidney deficiency type, and assisting in the treatment of stress obesity.

[0086] (6) Mouse GABA

[0087] As Figure 6 shown, compared with group 1, the contents of GABA in the hypothalamus tissues and serum of group 2 mice were both decreased, and the differences were statistically significant (P<0.05). Compared with group 2, the contents of GABA in the hypothalamus tissues of group 3, group 4, and group 5 mice were all increased, and the differences were statistically significant (P<0.05); the contents of GABA in the serum of group 3, group 4, and group 5 mice were all increased, and the differences between group 4 and group 5 were statistically significant (P<0.05). It shows that the medicinal essential oil of the present invention has the effect of regulating GABA, and GABA has a regulatory effect on emotions and body weight, which may be the potential pharmacodynamic mechanism of the traditional Chinese medicine essential oil of the present invention in treating stress obesity of lung and kidney deficiency type. At the same time, compared with group 5, the contents of GABA in the hypothalamus tissues and serum of group 6 and group 7 mice were both decreased, and the differences were statistically significant (P<0.05).

[0088] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification.

[0089] The above-described embodiments only represent several implementation manners of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the invention patent of the present invention should be subject to the appended claims.

Claims

1. A traditional Chinese medicine composition for treating stress obesity, characterized in that, The active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 15-45 parts of Rhodiola rosea, 15-45 parts of Saussurea involucrata, 5-15 parts of cinnamon, 5-15 parts of sandalwood, and 10-30 parts of Inula racemosa.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 20-40 parts of Rhodiola rosea, 20-40 parts of Saussurea involucrata, 6-12 parts of cinnamon, 6-12 parts of sandalwood, and 15-25 parts of Inula racemosa.

3. The preparation method of the traditional Chinese medicine composition according to any one of claims 1 to 2, characterized in that, Comprising the following steps: Weigh each raw material according to the dosage, and extract with ethanol for 1-3 times; Combine the filtrates, remove the solvent, and obtain the traditional Chinese medicine composition.

4. The preparation method according to claim 3, characterized in that, The temperature of the extraction is 50°C-60°C, and the time is 1.5h-4h; and / or, The volume fraction of the ethanol is 85%-100%; and / or, The ratio of the total weight of the raw materials to the ethanol is 1:(5-10) g / mL; and / or, The method for removing the solvent is vacuum concentration; and / or, The relative density of the traditional Chinese medicine composition is 0.90-1.

05.

5. Use of the traditional Chinese medicine composition according to any one of claims 1-2 in the preparation of a drug for treating stress obesity.

6. A drug for treating stress obesity, characterized in that, The active ingredients of the drug comprise the traditional Chinese medicine composition according to any one of claims 1-2.

7. The drug according to claim 6, characterized in that, The dosage form of the drug is an external dosage form, preferably an essential oil.

8. The drug according to claim 7, characterized in that, The essential oil is a base oil containing the traditional Chinese medicine composition, and the volume fraction of the traditional Chinese medicine composition in the essential oil is 1%-5%.

9. The drug according to claim 8, characterized in that, The base oil includes at least one of sweet almond oil, safflower oil, coconut oil, linseed oil, and grape seed oil.

10. The preparation method of the drug according to claim 8 or 9, characterized in that, Comprising the following steps: Add the traditional Chinese medicine composition to the base oil and mix evenly to obtain the product.

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