Sugar-controlling composition containing rhodiola rosea extract, preparation method of sugar-controlling composition and application of sugar-controlling composition in health-care products

Abstract

The invention relates to a sugar-controlling composition containing a rhodiola rosea extract, a preparation method of the sugar-controlling composition and application of the sugar-controlling composition in health-care products, and relates to the technical field of functional foods. The sugar control composition containing the rhodiola rosea extract is prepared from the following components in parts by weight: 5 to 15 parts of rhodiola rosea extract, 10 to 20 parts of radix astragali seu hedysari extract, 5 to 15 parts of bitter gourd extract, 3 to 10 parts of cortex cinnamomi extract, 3 to 8 parts of folium mori extract, 2 to 8 parts of rhizoma coptidis extract, 2 to 5 parts of grape seed extract, 20 to 40 parts of rhizoma dioscoreae powder and 8 to 15 parts of adhesive. The rhodiola rosea extract is taken as a core, the radix astragali extract, the bitter gourd extract, the cortex cinnamomi extract, the folium mori extract, the rhizoma coptidis extract, the grape seed extract, the Chinese yam powder and the adhesive are compounded, a synergistic sugar control system is constructed, full-chain intervention on blood glucose metabolism is realized, and the components in the formula are medicinal and edible or natural extracts, so that the safety is high, and the effect is good. The long-term conditioning requirements are met.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of functional foods, in particular to a sugar control composition containing rhodiola rosea extract, a preparation method thereof and application thereof in health products. BACKGROUND

[0002] With the transformation of lifestyle and the intensification of population aging, the incidence of abnormal blood glucose (including prediabetes and type 2 diabetes) continues to rise globally, which has become a major public health challenge. Long-term uncontrolled blood glucose not only causes multiple complications such as diabetic nephropathy, retinopathy and cardiovascular disease, but also damages the function of pancreatic beta cells and the integrity of vascular endothelium through oxidative stress, inflammatory response and accumulation of glycated end products, forming a vicious cycle of abnormal blood glucose-organ damage-metabolic disorder, which seriously affects the quality of life and survival of patients.

[0003] Among the existing sugar control methods, chemical synthetic drugs such as metformin, sulfonylureas and DPP-IV inhibitors can quickly lower blood sugar, but they mostly target a single target such as inhibiting hepatic gluconeogenesis, promoting insulin secretion or prolonging the half-life of incretins, and long-term use may be accompanied by side effects such as gastrointestinal discomfort, risk of hypoglycemia, burden on liver and kidney function, etc. Natural products and ingredients with both medicinal and edible properties have gradually become the focus of research and development of sugar control products due to their high safety and multi-target regulation characteristics. SUMMARY

[0004] In order to solve the problems of insufficient target coverage and long-term use safety hazards of existing sugar control products, the present application optimizes the component ratio and preparation process by in-depth study of the active ingredient mechanism of each natural extract, and develops a multi-target synergistic, safe and mild sugar control composition.

[0005] In a first aspect, the present application provides a sugar control composition containing rhodiola rosea extract, which adopts the following technical solution: a sugar control composition containing rhodiola rosea extract, comprising the following components in parts by weight: 5-15 parts of rhodiola rosea extract, 10-20 parts of astragalus extract, 5-15 parts of bitter gourd extract, 3-10 parts of cinnamon extract, 3-8 parts of mulberry leaf extract, 2-8 parts of goldthread extract, 2-5 parts of grape seed extract, 20-40 parts of yam powder and 8-15 parts of adhesive.

[0006] The rhodioloside and quercetin in the rhodiolae et rosarum herba extract and the berberine in the coptidis rhizoma extract synergistically activate the AMPK pathway, the tyrosol in the rhodiolae et rosarum herba extract further regulates the expression of glucose transporter 4 in skeletal muscle and adipose tissue, and the PI3K-AKT signal transmission strengthened by the astragalus polysaccharide in the astragali radix extract and the procyanidins in the vitis vinifera seed extract forms synergy, accelerates the transport of glucose from blood to cells, and at the same time, the total nettle extract in the rhodiolae et rosarum herba extract regulates the balance between glycogen synthesis and decomposition in the liver, reduces hepatic gluconeogenesis, and together builds a three-dimensional glucose control system of liver-intestine-periphery, improves insulin resistance and promotes glucose transport, and comprehensively covers the core link of fasting blood glucose regulation.

[0007] The DNJ (1-deoxynojirimycin) in the mori folium extract and the cinnamic aldehyde in the cinnamomi cortex extract double inhibit the activity of intestinal carbohydrate enzymes, the momordica charantia extract regulates the intestinal flora through an insulin-like pathway, and the dietary fiber and oligosaccharides in the dioscoreae oppositae tuber powder delay sugar absorption and optimize the intestinal flora, forming a closed loop of postprandial blood glucose stable peak and sustained metabolic regulation.

[0008] Each component considers glucose control and body protection, and the total nettle extract, rhodioloside, tyrosol and other components in the rhodiolae et rosarum herba extract synergistically strengthen the antioxidant and anti-inflammatory effects, reduce the formation of glycation end products and the release of inflammatory factors, protect the function of pancreatic beta cells and vascular endothelium, and assist in regulating blood lipids and optimizing the intestinal flora, while the astragalus polysaccharide and dioscoreae oppositae tuber powder enhance the body's immunity; and the components of the formula are all homologous to medicine and food or natural extracts, which are safe and suitable for long-term glucose control and regulation needs.

[0009] Preferably, the mass ratio of the rhodiolae et rosarum herba extract and the coptidis rhizoma extract is (2-4):1.

[0010] By adopting the above technical scheme, when the amount of the coptidis rhizoma extract is too low, the berberine dose is insufficient, it is difficult to strongly activate the AMPK signal pathway, resulting in insufficient expression of glucose transporter and weakened gluconeogenesis inhibition, and the fasting blood glucose regulation effect is reduced; and the DPP-IV inhibition is insufficient, the half-life of incretins is shortened, the glucose control signal transmission of the intestinal-pancreatic axis is blocked, and the postprandial blood glucose stable peak ability is reduced; at the same time, the rhodiolae et rosarum herba extract is relatively excessive, and its mild regulation characteristics dominate, the glucose control effect is relatively delayed, and it is difficult to quickly respond to postprandial blood glucose fluctuations and suppress blood glucose peak value in time.

[0011] When the amount of the extract of Coptis chinensis is too high, the strong activation of the AMPK pathway by berberine is too strong, which can cause excessive uptake and utilization of glucose, and cause blood glucose to drop suddenly; at the same time, the amount of the extract of Rhodiola rosea is relatively insufficient, the promotion effect of quercetin contained in the extract of Rhodiola rosea on the secretion of GLP-1 is weakened, the synergistic regulation effect of berberine is weakened, the probability of rebound of blood glucose in the later stage is increased, and it is difficult to maintain long-term blood glucose homeostasis; therefore, after a large amount of research and experimental verification, the applicant finally determines that the mass ratio of the extract of Rhodiola rosea and the extract of Coptis chinensis in the present application is as above.

[0012] Preferably, the mass ratio of the extract of Momordica charantia and the extract of Cinnamomum cassia is (1-1.8):1.

[0013] By adopting the above technical solution, when the amount of the extract of Cinnamomum cassia is too low, the dosage of active ingredient cinnamaldehyde is insufficient, which makes it difficult to effectively improve the activity of insulin receptor substrate IRS-1, resulting in that the effect of momordica charantia glycoside on promoting the uptake of glucose by tissues is weakened, and the efficiency of blood glucose metabolism is decreased; at the same time, the insufficient inhibition of a small amount of cinnamaldehyde on intestinal sucrase and maltase reduces the effect of delaying carbohydrate absorption, and the postprandial blood glucose peak value is not well controlled; the amount of the extract of Momordica charantia is relatively high, and the excessive momordica charantia glycoside and its cold property can increase the burden on the gastrointestinal tract, affect the normal digestive function of the intestinal tract, indirectly interfere with the absorption and utilization of sugar control components, and further weaken the overall sugar control effect.

[0014] When the amount of the extract of Cinnamomum cassia is too high, the dosage of cinnamaldehyde is too high, which can cause the people with yin deficiency and excessive fire to have symptoms such as dry mouth, night sweats, and irritability, and reduce the range of the adaptive population; at the same time, the amount of the extract of Momordica charantia is relatively low, the dosage of momordica charantia glycoside is insufficient, the effect of insulin mimicry is weakened, the efficiency of glucose uptake and utilization is decreased, the postprandial blood glucose peak value is difficult to effectively suppress, and the amplitude of blood glucose fluctuation is enlarged; therefore, after a large amount of research and experimental verification, the applicant finally determines that the mass ratio of the extract of Momordica charantia and the extract of Cinnamomum cassia in the present application is as above.

[0015] Preferably, the mass ratio of the extract of Astragalus membranaceus and the extract of Vitis vinifera is (3-5):1.

[0016] By adopting the above technical solution, when the amount of the extract of Vitis vinifera is too low, the dosage of proanthocyanidin is insufficient, which makes it difficult to effectively scavenge active oxygen caused by high blood glucose, resulting in that the Nrf2 antioxidant pathway cannot be fully up-regulated, the risk of damage to pancreatic beta cells by oxidative stress is increased, and the insulin secretion capacity is decreased; at the same time, the amount of the extract of Astragalus membranaceus is relatively high, and excessive astragalus polysaccharide can increase the digestive burden of the gastrointestinal tract, delay the absorption speed of the sugar control active components, cause the sugar control to take effect late, the improvement effect of insulin resistance is not good, and it is difficult to meet the core demand of sugar control.

[0017] When the dosage of grape seed extract is too high, the proanthocyanidins are excessive, and the strong antioxidant property thereof can interfere with the core sugar control pathway of other active ingredients, resulting in a decrease in the activation efficiency of the AMPK and PI3K-AKT pathways, an obstruction of the core sugar control links such as glucose transport and glycogen synthesis, and a weakening of the overall sugar control effect. Meanwhile, the dosage of the astragalus extract is relatively low, the astragalus polysaccharide dosage is insufficient, the repairing effect on islet beta cells is weakened, the expression amount of insulin receptors is insufficient, the insulin signal transduction efficiency is decreased, the insulin resistance state cannot be effectively improved, and the fasting blood glucose regulation effect is weakened. Therefore, the mass ratio of the astragalus extract and the grape seed extract in the present application is preferably the above.

[0018] Preferably, the mass ratio of the yam powder and the mulberry leaf extract is (4-7): 1.

[0019] By adopting the above technical solution, when the dosage of the mulberry leaf extract is too low, the DNJ dosage is insufficient, the inhibitory effect on intestinal alpha-glucosidase is weakened, the decomposition and absorption speed of carbohydrates such as starch and sucrose is accelerated, the postprandial blood glucose peak value is rapidly climbed, the blood glucose stable peak cannot be achieved, and the front-end interception effect of sugar control is weakened.

[0020] When the dosage of the mulberry leaf extract is too high, the excessive DNJ can excessively inhibit the activity of intestinal carbohydrate decomposition enzymes, resulting in insufficient absorption of carbohydrates, a rebound of blood glucose in the later stage, and a destruction of blood glucose homeostatic regulation. Meanwhile, the dosage of the yam powder is relatively low, the dosage of yam oligosaccharide is insufficient, the yam oligosaccharide cannot effectively proliferate beneficial bacteria such as bifidobacterium and bacteroides, compensate for the potential interference of DNJ on intestinal flora, and weaken the repair effect on the intestinal mucosal barrier and the secretion of intestinal incretins, the intestinal synergistic effect of sugar control is weakened, and the overall sugar control effect is decreased. Therefore, the mass ratio of the yam powder and the mulberry leaf extract in the present application is preferably the above.

[0021] Preferably, the binder is malt dextrin or starch.

[0022] By adopting the above technical solution, the malt dextrin and the starch are both food-grade raw materials, and do not have physiological activity interference, so as not to affect the sugar control mechanism of the extracts. Meanwhile, the hydrophilicity and the molding property of the malt dextrin and the starch are excellent, so as to ensure that the functional components are uniformly mixed and form stable particles, and the loss of active ingredients in the granulation process is minimized.

[0023] Preferably, the binder is malt dextrin.

[0024] Preferably, the weight part of the malt dextrin is 8-12 parts.

[0025] By adopting the technical scheme, the malt dextrin is used as a natural polysaccharide adhesive with a low DE value, has good biocompatibility and mild metabolic characteristics, the short-chain polysaccharide structure is slowly digested and absorbed in the intestinal tract, does not cause rapid fluctuation of blood sugar, does not conflict with the mechanism of the sugar control components such as rhodiola rosea and bitter gourd in the formula, can reduce the risk of rapid blood sugar drop by slowly supplying energy, and realizes the dual values of adhesion and sugar control adaptability.

[0026] When the amount of the malt dextrin is too low, a small amount of the malt dextrin cannot effectively bind the functional components, and problems such as particle fragmentation occur in the granulation process, which leads to uneven distribution of the sugar control active ingredients and unstable blood sugar regulation effect; when the amount of the malt dextrin is too high, the excessive adhesion leads to high particle hardness, which delays the dissolution rate of the active ingredients of the sugar control composition and makes it difficult to respond to the postprandial blood sugar rise in time; therefore, the applicant finally determines that the amount of the malt dextrin is preferably greater than 10 parts by weight.

[0027] In the second aspect, the application provides a preparation method of the sugar control composition containing the rhodiola rosea extract, which adopts the following technical scheme: A preparation method of a sugar control composition containing a rhodiola rosea extract, for preparing the above-mentioned sugar control composition containing the rhodiola rosea extract, comprising the following steps: S1. uniformly mixing the formula amount of the rhodiola rosea extract, the astragalus extract, the bitter gourd extract, the cinnamon extract, the mulberry leaf extract, the goldthread extract, the grape seed extract and the yam powder to obtain a mixture; S2. taking the formula amount of the adhesive, if the adhesive is a water-soluble adhesive, adding water and stirring until the adhesive is completely dissolved; if the adhesive needs to be activated, adding water, stirring and heating to gelatinize, and cooling for standby; obtaining a pretreated adhesive; S3. adding the pretreated adhesive to the mixture, uniformly mixing and granulating, and drying to obtain the sugar control composition containing the rhodiola rosea extract.

[0028] In the third aspect, the application provides a health product, which adopts the following technical scheme: A health product comprising the above-mentioned sugar control composition containing the rhodiola rosea extract, and the health product has excellent blood sugar regulation performance.

[0029] In summary, the application has the following beneficial effects: 1. The present application takes rose rhodiola extract as the core, and is compounded with astragalus extract, bitter gourd extract, cinnamon extract, mulberry leaf extract, goldthread extract, grape seed extract, yam powder and adhesive to construct a synergistic sugar control system, realize the whole chain intervention of blood glucose metabolism, and the formula components are all homologous to medicine and food or natural extracts, which are safe and suitable for long-term conditioning needs; 2. The present application activates the AMPK pathway by the synergy of rhodioside in rose rhodiola extract, quercetin and berberine in goldthread extract, accelerates the transport of glucose from blood to cells by the synergy of tyrosol and astragalus polysaccharide in astragalus extract and procyanidin in grape seed extract, adjusts the balance of liver glycogen synthesis and decomposition, reduces hepatic gluconeogenesis, and together constructs a three-dimensional sugar control system of liver-intestine-peripheral to comprehensively cover the core link of fasting blood glucose regulation; 3. The present application double inhibits the activity of intestinal carbohydrate-degrading enzymes by DNJ in mulberry leaf extract and cinnamic aldehyde in cinnamon extract, simulates the effect of insulin by momordica charantia in bitter gourd extract, delays sugar absorption and optimizes intestinal flora by dietary fiber and oligosaccharides in yam powder, forms a closed loop of stable postprandial blood glucose peak and sustained metabolic regulation; 4. The present application preferably uses malt dextrin as adhesive, which is a low DE value natural polysaccharide adhesive with good biocompatibility and mild metabolic characteristics, and will not cause rapid blood glucose fluctuation, but also can reduce the risk of blood glucose sudden drop by slow energy supply, realizing the dual value of adhesive function and sugar control adaptability. DETAILED DESCRIPTION

[0030] The extract in the present application includes the following parts: Preparation Example 1 The preparation method of rose rhodiola extract includes the following steps: A1. Crush the clean rose rhodiola rhizome to 20-40 mesh, then add 8 times the weight of ethanol solution, wherein the mass concentration of the ethanol solution is 70wt%, to obtain a rhodiola mixture; A2. Ultrasonic the rhodiola mixture at 50℃ for 40min, wherein the ultrasonic power is 300W, filter to obtain rhodiola filter residue and first rhodiola extract, then add the same mass and concentration of ethanol solution as in step A1 to the rose rhodiola filter residue, ultrasonic again, filter to obtain second rose rhodiola extract, combine the first rose rhodiola extract and the second rose rhodiola extract to obtain rose rhodiola extract; A3. Filter the rose rhodiola extract through a 300-mesh filter cloth, then adsorb the filtrate through Ab-8 macroporous resin with a flow rate of 1BV / h, then sequentially elute the impurities with 3BV of water and 3BV of 10wt% ethanol solution, and then elute the target components with 5BV of 70wt% ethanol solution, and collect the rose rhodiola target component eluate; A4. The rose rhodiola target component eluent is concentrated under reduced pressure, the concentration conditions are 60°C and a vacuum degree of -0.08 MPa, then the concentrated product is vacuum freeze-dried, the drying parameters are pre-freezing at -40°C for 2 h, a vacuum degree of 10 Pa, and sublimation drying for 12 h, then the dried product is crushed to 80 mesh, and sieved to obtain the rose rhodiola extract.

[0031] Preparation Example 2 The preparation method of the astragalus extract includes the following steps: B1. Clean mongolian astragalus root is crushed to 20-40 mesh, then 10 times the weight of water is added to obtain an astragalus mixture; B2. The astragalus mixture is reflux extracted at 90°C for 1.5 h, filtration is performed to obtain astragalus filter residue and first astragalus extract, then the same weight of distilled water as in step B1 is added to the astragalus filter residue, and water bath reflux extraction is performed again, filtration is performed to obtain second astragalus extract; the first astragalus extract and the second astragalus extract are combined to obtain the astragalus extract; B3. The astragalus extract is filtered through a 300-mesh filter cloth, the filtrate is concentrated under reduced pressure, the concentration conditions are 55°C and a vacuum degree of -0.08 MPa, and the astragalus concentrated solution is obtained; then 3 times the weight of 95wt% ethanol solution is added to the astragalus concentrated solution for alcohol precipitation, and the alcohol precipitation product is obtained by standing at 4°C for 12 h; B4. The alcohol precipitation product is centrifuged at a speed of 3000 r / min for 15 min, and the lower precipitate is washed with 80wt% ethanol solution, and the centrifugation-washing is repeated twice to remove monosaccharides and impurities; B5. The washed precipitate is redissolved with water, then spray dried under the conditions of an inlet air temperature of 180°C, an outlet air temperature of 80°C, and a flow rate of 5 mL / min, then the dried product is crushed to 80 mesh, and sieved to obtain the astragalus extract.

[0032] Preparation Example 3 The preparation method of the bitter gourd extract includes the following steps: C1. Clean bitter gourd is crushed to 20-40 mesh, then 6 times the weight of ethanol solution is added, wherein the mass concentration of the ethanol solution is 50wt%, to obtain a bitter gourd mixture; C2. The bitter gourd mixture is stirred at 60°C for 1 h, filtration is performed to obtain bitter gourd filter residue and first bitter gourd extract; then the same weight and concentration of ethanol solution as in step C1 is added to the bitter gourd filter residue, stirring is performed again, and filtration is performed to obtain second bitter gourd extract; the first bitter gourd extract and the second bitter gourd extract are combined to obtain the bitter gourd extract; C3. The balsam pear extract liquid is filtered through a 300-mesh filter cloth, and the filtrate is concentrated under reduced pressure. The concentration conditions are 60°C and a vacuum degree of -0.08 MPa. The concentrated liquid is then adsorbed through D101 macroporous resin, and the adsorption flow rate is controlled at 2 BV / h. Then, the target component is eluted with 5 BV of water, and then eluted with 4 BV of an ethanol solution with a mass concentration of 60 wt%. The balsam pear target component eluate is collected; C4. The balsam pear target component eluate is spray dried under the following conditions: an inlet air temperature of 170°C and an outlet air temperature of 75°C. Then, the dried product is crushed to 80 mesh, and sieved to obtain the balsam pear extract.

[0033] Preparation Example 4 A preparation method of a cassia extract includes the following steps: D1. Clean cassia bark is crushed to 20-40 mesh, and then 8 times the weight of an ethanol solution is added, wherein the mass concentration of the ethanol solution is 95 wt%. A cassia mixture is obtained; D2. The cassia mixture is extracted at 80°C under reflux for 1 h in the dark, and a condensation reflux device is simultaneously started to prevent the volatilization of cinnamic aldehyde. Filtration obtains cassia residue and a first cassia extract. The same mass and concentration of ethanol solution as in step D1 is added to the cassia residue, and the extraction is performed again in the dark. Filtration obtains a second cassia extract. The first cassia extract and the second cassia extract are combined to obtain a cassia extract; D3. The cassia extract is filtered through a 300-mesh filter cloth, and the filtrate is concentrated under reduced pressure. The concentration conditions are 50°C and a vacuum degree of -0.09 MPa. Then, 3 times the weight of ethyl acetate is added to the concentrated product for dissolution. The insoluble substances are removed by filtration, and the cassia target product filtrate is collected; D4. The cassia target product filtrate is distilled under reduced pressure. The heating temperature is set to 40°C, and the vacuum degree is -0.09 MPa. Ethyl acetate is recovered to obtain a cassia extract crude residue. The cassia extract crude residue is then vacuum dried under the following conditions: 50°C and a vacuum degree of -0.08 MPa. The dried product is crushed to 80 mesh, and sieved to obtain the cassia extract.

[0034] Preparation Example 5 A preparation method of a mulberry leaf extract includes the following steps: E1. Clean mulberry leaves are crushed to 20-40 mesh, and then 10 times the weight of water is added to obtain a mulberry leaf mixture; E2. The mulberry leaf mixture is extracted in a water bath at 80°C for 1 h. Filtration obtains mulberry leaf residue and a first mulberry leaf extract. The same weight of distilled water as in step E1 is added to the mulberry leaf residue, and the water bath is started again. Filtration obtains a second mulberry leaf extract. The first mulberry leaf extract and the second mulberry leaf extract are combined to obtain a mulberry leaf extract; E3. The mulberry leaf extract liquid is filtered through a 300-mesh filter cloth, and then the filtrate is adsorbed by a 732 type cation exchange resin, with an adsorption flow rate controlled at 1 BV / h; then 5 BV of water is used to elute the impurities, and then 3 BV of an ammonia solution with a mass concentration of 2 wt% is used to elute the target components of the mulberry leaf, the eluate of the target components of the mulberry leaf is collected and adjusted to a pH of 6.0, to obtain an eluate after purification; E4. The eluate after purification is concentrated under reduced pressure, with a concentration condition of 50°C and a vacuum degree of -0.09 MPa, and then vacuum freeze-dried, with a drying parameter of pre-freezing at -40°C for 2.5 h, a vacuum degree of 10 Pa, and sublimation drying for 15 h, and then the dried product is crushed to 80 mesh, and sieved to obtain the mulberry leaf extract.

[0035] Preparation Example 6 A preparation method of the extract of the Chinese goldthread rhizome includes the following steps: F1. Clean Chinese goldthread rhizomes are crushed to 20-40 mesh, and then 10 times the weight of a sulfuric acid solution is added, with a mass concentration of 0.5 wt%, to obtain a Chinese goldthread mixture; F2. The Chinese goldthread mixture is stirred at 70°C for 1 h to obtain a Chinese goldthread residue and a first Chinese goldthread extract liquid; the same weight and concentration of the sulfuric acid solution as in step F1 is added to the Chinese goldthread residue, and stirring is performed again, and a second Chinese goldthread extract liquid is obtained by filtration; the first Chinese goldthread extract liquid and the second Chinese goldthread extract liquid are combined to obtain a Chinese goldthread extract liquid; F3. The Chinese goldthread extract liquid is filtered through a 300-mesh filter cloth, and a sodium hydroxide solution with a mass concentration of 10 wt% is used to adjust the pH of the filtrate to 10.0, and the mixture is left to stand for 2 h and centrifuged at 3000 r / min for 15 min, and the lower precipitate is dissolved in an ethanol solution with a mass concentration of 50 wt%, and the solution is filtered and concentrated under reduced pressure, with a concentration condition of 50°C and a vacuum degree of -0.09 MPa, to obtain a Chinese goldthread concentrated product.

[0036] F4. The Chinese goldthread concentrated product is air-dried at 60°C, and then crushed to 80 mesh to obtain a dry powder, and sieved to obtain the Chinese goldthread extract.

[0037] Preparation Example 7 A preparation method of the grape seed extract includes the following steps: G1. Clean grape seeds are crushed to 20-40 mesh, and then 6 times the weight of an acetone solution is added, with a mass concentration of 60 wt%, to obtain a grape seed mixture; G2. The grape seed mixture was extracted at 40℃ under ultrasonic for 30 min in the dark, with an ultrasonic power of 200W. The filtrate was obtained by filtration, and the grape seed residue was obtained. The grape seed residue was added with the same amount and concentration of acetone solution as in step G1. The second grape seed extract was obtained by ultrasonic extraction and filtration. The first grape seed extract and the second grape seed extract were combined to obtain the grape seed extract; G3. The grape seed extract was filtered through a 300-mesh filter cloth, and then the filtrate was recovered under reduced pressure to recover acetone. The temperature was set to 40℃, and the vacuum degree was set to -0.08MPa. The residual solution was obtained, and then the same volume of ethyl acetate as the residual solution was added for extraction. The extraction was repeated 3 times, and the extraction products after 3 times of extraction were combined to obtain the grape seed clear organic phase; G4. The grape seed clear organic phase was concentrated under reduced pressure. The concentration conditions were temperature 35℃ and vacuum degree -0.08MPa. The grape seed extract crude product residue was obtained, and then the grape seed extract crude product residue was dried in the dark under vacuum. The drying conditions were 40℃ and vacuum degree -0.08MPa. The dried product was pulverized to 80 mesh, and then sieved to obtain the grape seed extract.

[0038] Preparation Example 8 A preparation method of yam powder, comprising the following steps: uniformly cutting sulfur-free yam tablets into 2mm thin slices, drying at 60℃ for 2h, turning over and continuing to dry for 2h, and then ultrafine grinding to 100 mesh, and sieving to obtain yam powder.

[0039] The application will be further described in detail below in combination with examples and comparative examples.

[0040] Example 1 A preparation method of a sugar control composition containing rose rhodiola extract, comprising the following steps: S1. 120g of rose rhodiola extract prepared in Preparation Example 1, 140g of astragalus extract prepared in Preparation Example 2, 90g of bitter gourd extract prepared in Preparation Example 3, 60g of cinnamon extract prepared in Preparation Example 4, 50g of mulberry leaf extract prepared in Preparation Example 5, 40g of coptis extract prepared in Preparation Example 6, 35g of grape seed extract prepared in Preparation Example 7, and 300g of yam powder prepared in Preparation Example 8 were mixed, with a stirring speed of 15r / min and a stirring time of 20min, to obtain a uniformly dispersed mixture; S2. 100g of malt dextrin was added with 500g of water, and stirred at a stirring speed of 500r / min at room temperature for 10min to obtain a pretreated binder; S3. Stir the mixture at 30 r / min, and spray the pretreated binder on the mixture, continuously stir for 30 min, until the material forms a uniform soft material, granulate through a 16 mesh screen, to obtain wet granules; then dry the wet granules, set the drying temperature to 60°C, the air speed to 1.5 m / s, dry for 2 h, turn the material over every 30 min during the drying, and cool and sieve to obtain the rose rhodiola extract-containing sugar-controlling composition.

[0041] Example 2-3 Example 2-3 is based on the preparation method of Example 1, and the addition amount of each component in the rose rhodiola extract-containing sugar-controlling composition is adjusted, and the specific adjustment is shown in Table 1.

[0042] Comparative Example 1-8 Comparative Example 1-8 is based on the preparation method of Example 1, and the addition amount of each component in the rose rhodiola extract-containing sugar-controlling composition is adjusted, and the specific adjustment is shown in Table 1-1.

[0043] Table 1-1 Raw material table of Example 1-3 and Comparative Example 1-8 (unit: g) The rose rhodiola extract-containing sugar-controlling composition prepared in Example 1-3 and Comparative Example 1-8 is subjected to the following performance detection, and the detection results are shown in Table 1-2.

[0044] Performance detection test: a. Test object: healthy six-week-old male C57BL / 6J mice (purchased from Sibeifeng Biotechnology Co., Ltd.), body weight 18-20 g, free feeding and drinking during animal feeding, room temperature maintained at 20-25°C, humidity 40%-60%.

[0045] b. Configuration of main test reagents: b1. Sample preparation: weigh 0.125 g of the rose rhodiola extract-containing sugar-controlling composition, configure into a 6.25 mg / mL sample suspension with 20 mL of a 0.5 wt% carboxymethylcellulose sodium solution, and filter once through an 80 mesh screen, and give 0.1 mL / 25 g to each mouse by gavage.

[0046] b2. Streptozotocin solution: under light-proof conditions, weigh 0.25 g of streptozotocin powder, dissolve into a 12.5 mg / mL streptozotocin solution with 20 mL of a 0.1 mol / L citric acid buffer, and give 0.1 mL / 25 g to each mouse by intraperitoneal injection.

[0047] b3. Glucose solution preparation: 2 g of anhydrous glucose powder was weighed and dissolved in 10 mL of 0.9% physiological saline solution to prepare a glucose solution with a mass concentration of 20 wt%. Each mouse was given 10 μL / g by gavage.

[0048] c. Establish a mouse administration model: c1. The mice adapted for 3 days were randomly divided into cages: 10 mice in the blank control group were fed with normal maintenance feed; 20 mice in the experimental group were fed with a diabetic high-fat special feed, in which the fat energy supply ratio was 41%, the carbohydrate energy supply ratio was 43%, the protein energy supply ratio was 17%, and 0.15% cholesterol was additionally added.

[0049] c2. After continuous feeding for 4 weeks and the body weight reached 30-35 g, the mice in the blank control group were injected with an equal amount of normal saline; the mice in the experimental group were injected with streptozotocin solution intraperitoneally, and the injection was continued for 5 days. When the fasting blood glucose was ≥11.1 mol / L and the random blood glucose was ≥16.7 mol / L, it was proved that the modeling was successful.

[0050] c3. After the modeling was successful, the mice were divided into groups for administration (once a day, for 28 consecutive days) as follows: The blank control group was given 0.5 wt% carboxymethylcellulose sodium by gavage; The mice in the experimental group were randomly divided into 2 groups, 10 mice in the model group were given 0.5 wt% carboxymethylcellulose sodium by gavage; 10 mice in the sample group were given 0.1 mL / 25 g of sample suspension by gavage.

[0051] d. Performance detection index: d1. Fasting blood glucose: On the 15th day and the 28th day after administration, the fasting blood glucose value of the mice was recorded for 12 h, and the mice were given normal water during fasting. The mice were taken out of the cage, disinfected, and then a blood collection needle was inserted into the tail end of the mouse about 1-2 mm. The mouse's tail was gently squeezed to allow the blood to accumulate into a drop, and the fasting blood glucose before administration was measured with a blood glucose meter. The fasting blood glucose of the blank control group during administration was maintained at 5.90-7.50 mmol / L, and the fasting blood glucose of the model group during administration was maintained at 16.10-17.20 mmol / L.

[0052] d2. Oral glucose tolerance test (OGTT): The experiment was conducted on the 26th day. After fasting for 12 h, the mice were given 20% glucose solution (2 g / kg BW) by gavage. The tail vein blood glucose value was measured before administration (0 min) and 15 min, 30 min, 60 min, 90 min, and 120 min after administration (recorded as G0, G 15 , G30 , G 60 , G 90 , G 120 ).

[0053] The area under the curve was calculated by trapezoidal rule to evaluate the glucose tolerance level of mice, and the formula was OGTT AUC =∑[(G t1 +G t2 )×Δt / 2] Wherein, G t1 and G t2 are the blood glucose values of two adjacent time points, and Δt is the adjacent time interval (unit: h). The OGTT AUC value of the blank control group was 65.74 mmol / L·h, and the OGTT AUC value of the model group was 132.62 mmol / L·h; Note: The higher the OGTT AUC value, the weaker the body's ability to remove glucose from the blood; the lower the OGTT AUC value, the more normal the glucose regulation function.

[0054] d3. Serum insulin level: Serum collection: The mice were fasted for 12 h before being sacrificed, and the eyeball was removed to collect blood into a 1.5 mL centrifuge tube before being centrifuged at 3000 rpm for 15 min at 4°C. The upper serum was separated to obtain the serum sample and stored at -80°C; Detection steps: d31. The sample and insulin (INS) detection kit components were warmed to room temperature for 120 min (the kit was purchased from Quanzhou Ruixin Biological Technology Co., Ltd., with a specification of RXW202485M, 96T), and the plate strip was set: Standard well: 50 μL of insulin standard solution with concentrations of 0 mIU / L, 1.25 mIU / L, 2.5 mIU / L, 5 mIU / L, 10 mIU / L, 20 mIU / L, and 40 mIU / L was added in order of concentration gradient; 0 value well: 50 μL of sample diluent in the kit was added; Blank well: no liquid was added; Sample well: 40 μL of sample diluent in the kit was first added to each well, and then 10 μL of serum sample to be tested was added; d32. Except for the blank well, 100 μL of HRP (horseradish peroxidase) labeled detection antibody was added to the standard well, 0 value well, and sample well, and then the reaction plate strip was covered with a sealing film. The plate was incubated in a 37°C incubator for 60 min in the dark; d33. After the end of the incubation, pour out the liquid in the hole, add the diluted washing liquid to each hole, and shake off the washing liquid after 20s. Place the plate upside down on the water-absorbing paper and pat dry. Repeat the washing for 5 times; d34. Mix the substrate A liquid and the substrate B liquid in the kit at a volume ratio of 1:1. Then add 100 μL of the mixed substrate liquid to each reaction hole. Then cover the reaction plate with a sealing film. Incubate in a 37°C constant temperature incubator for 15 min in the dark. d35. After the end of the incubation, add 50 μL of the termination liquid in the kit to each hole. Read the absorbance (OD value) of each hole on an enzyme label instrument at 450 nm.

[0055] Result calculation: Take the standard concentration as the abscissa (6 standard holes, one 0 hole, and a total of 7 concentration points), and the OD value as the ordinate. A four-parameter Logistic curve fitting (4-pl) is used to establish a standard curve equation. The sample OD value is substituted into the equation to calculate the concentration value of the sample. The serum insulin concentration level of the blank control group was 45.17 mIU / L; the serum insulin concentration level of the model group was 27.74 mIU / L.

[0056] Table 1-2 Performance detection table of examples 1-3 and comparative examples 1-8 Referring to Table 1-1 and Table 1-2, it can be seen that the fasting blood glucose and OGTT AUC values of comparative example 1-8 are higher than those of example 1-3, and the serum insulin concentration levels are lower than those of example 1-3. This is because comparative example 1-8 is missing one of rhododendron extract, astragalus extract, bitter gourd extract, cinnamon extract, mulberry leaf extract, coptis extract, grape seed extract, and yam powder, which breaks the core synergistic effect of activating the AMPK pathway, strengthening the PI3K-AKT signal transduction, synergistically inhibiting intestinal carbohydrate-degrading enzymes, protecting islet beta cells, and optimizing intestinal flora. Ultimately, the fasting blood glucose and OGTT AUC values rise and the serum insulin concentration decreases.

[0057] By comparison, the comprehensive performance of the sugar control composition containing rhododendron extract in example 1 is the best, so example 1 is preferred.

[0058] Examples 4-7 Examples 4-7 are based on the preparation method of example 1, keeping the mass of rhododendron extract and coptis extract as 160 g unchanged, and adjusting the mass ratio of rhododendron extract and coptis extract. The specific adjustments are shown in Table 2.

[0059] The glucose control composition containing rose rhodiola extract prepared in Examples 4-7 was subjected to the performance test as above, and the test results are shown in Table 2.

[0060] Table 2 Quality ratio of rose rhodiola extract and coptis extract and performance test table of Examples 1 and 4-7 Referring to Table 2, it can be seen from Comparative Example 1 and Examples 4-7 that the use amount of coptis extract is too low or too high, which will cause the performance of the glucose control composition containing rose rhodiola extract to decrease. When the use amount of coptis extract is too low, the dose of berberine is insufficient, and it is difficult to synergistically activate the AMPK pathway with rose rhodiola extract, the glucose transport efficiency decreases, the fasting blood glucose increases, and at the same time, the regulatory effect of enteropancreatic hormone is insufficient, the insulin secretion synergy after meal decreases, the postprandial blood glucose clearance ability decreases, the OGTT AUC value increases, and the protective effect on islet beta cells weakens, the serum insulin concentration is low, and the overall blood glucose regulation effect weakens.

[0061] When the use amount of coptis extract is too high, excessive berberine will strongly activate the AMPK pathway, trigger the compensatory rebound after excessive glucose uptake, and limit the decrease of 28d fasting blood glucose; at the same time, it competes with quercetin for target points, interferes with the postprandial blood glucose regulation synergy mechanism, and decreases the postprandial blood glucose clearance ability, and increases the OGTT AUC value; excessive berberine also interferes with the absorption of other glucose control components, leading to insufficient repair of islet beta cells and insufficient insulin secretion, resulting in a decrease in serum insulin concentration, and it is difficult to maintain a stable blood glucose regulation state.

[0062] Examples 8-11 Examples 8-11 are prepared on the basis of the preparation method of Example 1, the mass of momordica charantia extract and cinnamomum cassia extract is kept unchanged at 150g, and the mass ratio of momordica charantia extract and cinnamomum cassia extract is adjusted, and the specific adjustment is shown in Table 3.

[0063] The glucose control composition containing rose rhodiola extract prepared in Examples 8-11 was subjected to the performance test as above, and the test results are shown in Table 3.

[0064] Table 3 Mass ratio of momordica charantia extract and cinnamomum cassia extract of Examples 1 and 8-11 and performance test table Referring to Table 3, it can be seen from Comparative Example 1 and Examples 8-11 that the amount of the cinnamon extract is too low or too high, which can result in the decrease of the performance of the sugar control composition containing the rhodiola rosea extract. When the amount of the cinnamon extract is too low, the activity of the insulin receptor substrate IRS-1 cannot be effectively improved due to the insufficient cinnamaldehyde, which results in the decrease of the glucose uptake effect of the momorcharin and the increase of the fasting blood glucose. Meanwhile, the inhibition of the intestinal carbohydrate enzyme is insufficient, the carbohydrate absorption in the postprandial period is accelerated, the OGTT AUC value is increased. Moreover, the synergistic protection of the pancreatic beta cells is weakened, the serum insulin concentration is low, and the sugar control effect is decreased.

[0065] When the amount of the cinnamon extract is too high, the insulin mimetic effect of the momorcharin is weakened due to the relative insufficiency of the momorcharin, the glucose uptake efficiency is decreased, which results in the limited decrease of the fasting blood glucose after 28 days. The excessive cinnamaldehyde can interfere with the synergistic sugar control effect between the other components, which reduces the postprandial blood glucose clearance capacity of the body, results in the increase of the OGTT AUC value, and the direct protection of the pancreatic beta cells by the momorcharin is weakened, which results in the insufficient increase of the insulin secretion and the low serum insulin concentration, and it is difficult to maintain the blood glucose homeostasis.

[0066] Examples 12-15 Examples 12-15 are prepared based on the preparation method of Example 1, the mass of the astragalus extract and the grape seed extract is kept unchanged at 175 g, and the mass ratio of the astragalus extract and the grape seed extract is adjusted, and the specific adjustment is shown in Table 4.

[0067] The sugar control composition containing the rhodiola rosea extract prepared in Examples 12-15 is subjected to the performance detection as above, and the detection results are shown in Table 4.

[0068] Table 4. The mass ratio of the astragalus extract and the grape seed extract and the performance detection table of Example 1 and Examples 12-15 Referring to Table 4, it can be seen from Comparative Example 1 and Examples 12-15 that the amount of the grape seed extract is too low or too high, which can result in the decrease of the performance of the sugar control composition containing the rhodiola rosea extract. When the amount of the grape seed extract is too low, the dose of the proanthocyanidin is insufficient, which results in the low serum insulin concentration due to the insufficient protection of the pancreatic beta cells by the active oxygen. Meanwhile, the excessive astragalus polysaccharide increases the burden of the gastrointestinal tract, the absorption of the sugar control components is delayed, the fasting blood glucose is not well decreased, the postprandial blood glucose clearance capacity is weakened, and the OGTT AUC value is increased.

[0069] When the dosage of grape seed extract is too high, its strong antioxidant property can interfere with the core sugar control pathways such as AMPK, PI3K-AKT, etc., leading to an increase in fasting blood glucose; at the same time, the relative lack of astragalus polysaccharide weakens the repair of islet beta cells and the promotion of insulin receptor expression, and the increase in serum insulin concentration is limited; the postprandial blood glucose clearance capacity decreases, OGTT AUC value increases, and it is difficult to maintain blood glucose homeostasis.

[0070] Examples 16-19 Examples 16-19 are prepared on the basis of the preparation method of Example 1, the mass of yam powder and mulberry leaf extract is kept unchanged at 350 g, and the mass ratio of yam powder and mulberry leaf extract is adjusted, and the specific adjustment is shown in Table 5.

[0071] The sugar control composition containing rhodiola rosea extract prepared in Examples 16-19 is subjected to the performance detection as above, and the detection results are shown in Table 5.

[0072] Table 5 Mass ratio of yam powder and mulberry leaf extract and performance detection table of Examples 1 and 16-19 Referring to Table 5, it can be seen from Comparative Example 1 and Examples 16-19 that the performance of the sugar control composition containing rhodiola rosea extract decreases when the dosage of mulberry leaf extract is too low or too high, which is because when the dosage of mulberry leaf extract is too low, the dosage of DNJ is insufficient, the inhibition of intestinal alpha-glucosidase is weakened, the decomposition and absorption of postprandial carbohydrates are accelerated, the postprandial blood glucose metabolism efficiency decreases, leading to an increase in OGTT AUC value, and the overall sugar control synergy is weakened, and the fasting blood glucose regulation effect is not good.

[0073] When the dosage of mulberry leaf extract is too high, DNJ can excessively inhibit intestinal enzyme activity, leading to intestinal discomfort, affecting the absorption of other components, decreasing the postprandial blood glucose clearance capacity, and increasing the OGTT AUC value; at the same time, the relative lack of yam powder weakens the repair of intestinal mucosal barrier and the secretion of incretins, and the excessive DNJ can affect the function of islet beta cells, the serum insulin concentration is low, the fasting blood glucose regulation effect is limited, and it is difficult to maintain blood glucose homeostasis.

[0074] Examples 20-23 Example 20 is prepared on the basis of the preparation method of Example 1, in S2, the malt dextrin is replaced with starch, 500 g of water is added, the stirring speed is set to 500 r / min, after stirring at room temperature for 8 min, the temperature is heated to 75℃ in water bath while stirring, and the temperature is kept for 15 min to gelatinize the starch, and then cooled to below 40℃, to obtain the pretreated binder, and the remaining conditions are unchanged.

[0075] Examples 21-23 were prepared based on the preparation method of Example 1, and the amount of malt dextrin was adjusted, and the specific adjustment is shown in Table 6.

[0076] The glucose control composition containing rose rhodiola extract prepared in Examples 20-23 was subjected to performance detection as above, and the detection results are shown in Table 6.

[0077] Table 6 Amount of malt dextrin and performance detection table of Example 1 and Examples 20-23 Referring to Table 6, it can be seen from Comparative Example 1 and Example 20 that the fasting blood glucose, OGTT AUC value and serum insulin concentration of the glucose control composition containing rose rhodiola extract of Example 1 and Example 20 are relatively close, indicating that malt dextrin and starch can both be used as a binder for the glucose control composition containing rose rhodiola extract. Compared, the comprehensive performance of Example 1 is better, so Example 1 is preferred.

[0078] Comparative Example 1 and Examples 21-23, the amount of malt dextrin is too low or too high, which will cause the performance of the glucose control composition containing rose rhodiola extract to decrease. This is because when the amount of malt dextrin is too low, the components of each composition are difficult to uniformly bind, the active ingredients are unevenly distributed, leading to insufficient activation of the AMPK pathway, limited insulin secretion, slow decrease of fasting blood glucose; at the same time, the inhibition of intestinal carbohydrate enzymes is limited, the postprandial blood glucose clearance ability decreases, the OGTT AUC value increases, and the serum insulin concentration is insufficiently increased.

[0079] When the amount of malt dextrin is too high, the excessive malt dextrin dilutes the effective concentration of the glucose control active ingredients, and the synergistic regulation effect between the components, leading to an increase in fasting blood glucose; at the same time, it also interferes with the absorption efficiency of the active ingredients by the intestine, the postprandial blood glucose clearance ability decreases, the OGTT AUC value increases, and the serum insulin concentration is difficult to effectively increase, and the overall glucose control effect decreases.

[0080] The specific embodiments are merely an explanation of the present application, and are not a limitation of the present application. Those skilled in the art can make modifications to the embodiments without creative contribution after reading the present specification, and as long as the modifications are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A blood sugar-controlling composition containing Rhodiola rosea extract, characterized in that, It includes the following components by weight: 5-15 parts of Rhodiola rosea extract, 10-20 parts of Astragalus membranaceus extract, 5-15 parts of bitter melon extract, 3-10 parts of cinnamon extract, 3-8 parts of mulberry leaf extract, 2-8 parts of Coptis chinensis extract, 2-5 parts of grape seed extract, 20-40 parts of yam powder, and 8-15 parts of binder.

2. The blood sugar control composition containing Rhodiola rosea extract according to claim 1, characterized in that: The mass ratio of the Rhodiola rosea extract to the Coptis chinensis extract is (2-4):

1.

3. The blood sugar control composition containing Rhodiola rosea extract according to claim 1, characterized in that: The mass ratio of bitter melon extract to cinnamon extract is (1-1.8):

1.

4. The blood sugar control composition containing Rhodiola rosea extract according to claim 1, characterized in that: The mass ratio of Astragalus extract to grape seed extract is (3-5):

1.

5. The blood sugar control composition containing Rhodiola rosea extract according to claim 1, characterized in that: The mass ratio of the yam powder to the mulberry leaf extract is (4-7):

1.

6. The blood sugar control composition containing Rhodiola rosea extract according to claim 1, characterized in that: The binder is maltodextrin or starch.

7. The blood sugar control composition containing Rhodiola rosea extract according to claim 6, characterized in that: The adhesive is maltodextrin.

8. The blood sugar control composition containing Rhodiola rosea extract according to claim 7, characterized in that: The maltodextrin is present in 8-12 parts by weight.

9. A method for preparing a blood sugar-controlling composition containing Rhodiola rosea extract according to any one of claims 1-8, characterized in that, Includes the following steps: S1. Mix the prescribed amounts of Rhodiola rosea extract, Astragalus membranaceus extract, bitter melon extract, cinnamon extract, mulberry leaf extract, Coptis chinensis extract, grape seed extract, and yam powder evenly to obtain a mixture; S2. Take the amount of adhesive specified in the formula. If it is an easily soluble adhesive, add water and stir until completely dissolved. If it is an adhesive that needs to be activated, add water, stir, heat to gelatinize, and then cool for later use. The pretreated adhesive is obtained. S3. Add the pretreated binder to the mixture, mix evenly, granulate, and dry to obtain the sugar-controlling composition containing Rhodiola rosea extract.

10. A health product, characterized in that: Including the blood sugar control composition containing Rhodiola rosea extract as described in any one of claims 1-8.

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