Kidney-tonifying, hair-blackening, hair-increasing, anti-fatigue and body-strengthening medicine and preparation method thereof

Abstract

The invention relates to a medicine for tonifying kidney, blackening hair, increasing hair, resisting fatigue and strengthening body and a preparation method thereof, by combining 30 medicinal materials such as ginseng, penis et testis cervi and rhizoma polygonati and combining a modern extraction technology, the problems that a traditional prescription is low in absorptivity and slow in effect are solved, and the comprehensive effects of tonifying kidney, strengthening essence, blackening hair, strengthening hair and enhancing physical ability are synchronously achieved. The active ingredients are retained to the maximum extent by adopting a gradient extraction process, animal experiments prove that the composition can remarkably improve exercise tolerance and promote testosterone synthesis, and the composition is free of liver and kidney toxicity. The preparation can be developed into various dosage forms such as capsules, cream and the like, and has important application value in the field of sub-health conditioning.

Description

Technical Field

[0001] The present invention relates to a traditional Chinese medicine compound preparation for tonifying the kidney and anti - fatigue, and particularly to a drug for tonifying the kidney, blackening hair, increasing hair growth, and strengthening the body and its preparation method. Background Art

[0002] In the prior art, the formula homogenization is serious. 78% of the prescriptions contain traditional Chinese medicinal materials such as Epimedii Herba and Cistanches Herba, resulting in drug - resistance cases in clinical practice; the utilization rate of animal - based Chinese medicinal materials is low. Precious Chinese medicinal materials such as deer penis are mostly processed by wine - frying or directly crushed, and the utilization rate of their collagen is less than 12%; the modern pharmacological research is lacking, and the key signal pathway mechanisms such as AMPK / mTOR of the marketed products are not clear. At the same time, the loss of heat - sensitive components occurs. The traditional water - decocting process leads to a loss rate of cinnamaldehyde in Cinnamomi Ramulus reaching 82%; the interaction between components is ignored. It is found by detecting the medicinal residues that eucommia gum and polygonatum polysaccharide form complex precipitation, resulting in a 37% loss of active ingredients, as well as obstacles in industrial production. The energy consumption of the drying process of the mainstream products in the market is relatively high.

[0003] In recent years, studies have found that kidney essence deficiency not only leads to fatigue syndrome but is also closely related to premature graying and hair loss. Most of the existing kidney - tonifying traditional Chinese medicines focus on single efficacy and lack the coordinated regulation of hair health and physical fitness. Based on the traditional Chinese medicine theory of "the kidney governs bones and produces marrow, and its luster is manifested in the hair", the present invention synchronously realizes the triple effects of tonifying the kidney, blackening hair, and anti - fatigue through specific compatibility and process innovation.

[0004] The present invention is proposed based on a systematic solution to the above - mentioned technical defects through three - dimensional innovation of "components - process - quality control". Summary of the Invention

[0005] The purpose of this part is to outline some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Simplifications or omissions may be made in this part, as well as in the abstract and title of the present application, to avoid obscuring the purpose of this part, the abstract, and the title. However, such simplifications or omissions cannot be used to limit the scope of the present invention.

[0006] In view of the problems existing in the above - mentioned prior art, the inventor of the present invention has proposed the present invention.

[0007] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide a drug for tonifying the kidney, blackening hair, increasing hair growth, and strengthening the body and its preparation method.

[0008] To solve the above - mentioned technical problems, the present invention provides the following technical solution: A drug for tonifying the kidney, blackening hair, increasing hair growth, and strengthening the body, comprising:

[0009] Calculated by the mass parts of the drug raw materials for tonifying the kidney and anti - fatigue, it is composed of the following components,

[0010] 10 - 30 parts of ginseng, 5 - 15 parts of deer penis, 3 - 10 parts of deer placenta, 15 - 40 parts of polygonatum, 20 - 50 parts of wolfberry, 10 - 30 parts of eucommia ulmoides, 15 - 45 parts of mulberry, 5 - 20 parts of mulberry twig, 8 - 25 parts of clematis chinensis, 5 - 18 parts of cinnamon twig, 10 - 30 parts of citron, 10 - 35 parts of fingered citron, 5 - 15 parts of manqing, 10 - 30 parts of smoked plum, 20 - 60 parts of black bean, 3 - 12 parts of ganoderma spore powder, 15 - 40 parts of wild jujube seed, 10 - 30 parts of semen platycladi, 8 - 25 parts of peach kernel, 20 - 50 parts of millettia reticulata, 10 - 35 parts of plantain herb, 15 - 45 parts of dwarf lilyturf tuber, 5 - 20 parts of bupleurum chinense, 10 - 30 parts of cyathula officinalis, 8 - 25 parts of chuanxiong rhizome, 15 - 40 parts of dipsacus asperoides, 5 - 15 parts of andrographis paniculata, 10 - 30 parts of great burdock achene, 15 - 45 parts of cynomorium songaricum, 10 - 30 parts of cibotium barometz, 8 - 25 parts of lycopodium clavatum

[0011] As a preferred embodiment of the drug for tonifying the kidney, blackening hair, increasing hair growth, and enhancing physical fitness and relieving fatigue according to the present invention, wherein: the deer penis and deer placenta are treated by complex enzymatic hydrolysis, and the enzymatic hydrolysis conditions are: trypsin and flavor protease are mixed in a mass ratio of 1:2, the enzymatic hydrolysis temperature is 45 - 55 °C, pH is 6.0 - 7.0, and the time is 2 - 4 hours.

[0012] As a preferred embodiment of the drug for tonifying the kidney, blackening hair, increasing hair growth, and enhancing physical fitness and relieving fatigue according to the present invention, wherein: an inactivation step is added after enzymatic hydrolysis: the enzymatic hydrolysate is maintained at 85 - 90 °C for 10 - 15 minutes.

[0013] As a preferred embodiment of the drug for tonifying the kidney, blackening hair, increasing hair growth, and enhancing physical fitness and relieving fatigue according to the present invention, wherein: the volatile components in the drug are treated by β - cyclodextrin inclusion technology, and the inclusion rate is 85% - 95%.

[0014] The present invention also provides a preferred embodiment of a preparation method of a drug for tonifying the kidney, blackening hair, increasing hair growth, and enhancing physical fitness and relieving fatigue, including: (1) Pretreatment of animal medicinal materials: the deer penis and deer placenta are frozen and pulverized by liquid nitrogen and then enzymatically hydrolyzed;

[0015] (2) Extraction of plant medicinal materials: after the rhizome medicinal materials are ultrafinely pulverized to D90 ≤ 15 μm, they are extracted by dynamic counter - current extraction with 60 - 70% ethanol;

[0016] (3) Enrichment of active ingredients: the water extract is ultrafiltered through a ceramic membrane (cut - off molecular weight 50 - 100 kDa) and then spray - dried;

[0017] (4) Forming the preparation: the extract and medicinal adjuvants are made into capsules or tablets in a ratio of 1:0.3 - 0.5.

[0018] As a preferred embodiment of the method for preparing a drug for tonifying the kidney, blackening hair, increasing hair growth, anti - fatigue and strengthening the body according to the present invention, wherein: in step (2), the material - liquid ratio for dynamic counter - current extraction is 1:6 - 1:10, and the number of extraction times is 2 - 4 times.

[0019] As a preferred embodiment of the method for preparing a drug for tonifying the kidney, blackening hair, increasing hair growth, anti - fatigue and strengthening the body according to the present invention, wherein: in step (3), the operating pressure for ceramic membrane ultrafiltration is 0.2 - 0.5 MPa, and the membrane surface flow rate is 3 - 5 m / s.

[0020] As a preferred embodiment of the method for preparing a drug for tonifying the kidney, blackening hair, increasing hair growth, anti - fatigue and strengthening the body according to the present invention, wherein: in the preparation molding process of step (4), 0.1% - 0.5% magnesium stearate is added as a lubricant.

[0021] As a preferred embodiment of the method for preparing a drug for tonifying the kidney, blackening hair, increasing hair growth, anti - fatigue and strengthening the body according to the present invention, wherein: it also includes separately extracting the volatile components cinnamon twig and citron, collecting the distillate by steam distillation, and adding it to the final preparation after being included by β - cyclodextrin.

[0022] As a preferred embodiment of the method for preparing a drug for tonifying the kidney, blackening hair, increasing hair growth, anti - fatigue and strengthening the body according to the present invention, wherein: the mass ratio of β - cyclodextrin to the volatile component is 4:1 - 6:1, and the inclusion temperature is controlled at 35 - 45 °C.

[0023] The beneficial effects of the present invention: It first creates a "triple - tonifying system": animal essence + plant essence + fruit essence; introduces "two - way regulation" components: bupleurum regulates liver metabolism + wild jujube seed improves sleep quality + plantain herb balances damp - heat; composite enzymatic hydrolysis technology: uses trypsin + flavor protease to directionally decompose animal proteins; two - step fermentation process: uses Lactobacillus plantarum for pretreatment of medicinal materials; nano - embedding technology: uses β - cyclodextrin to include volatile components. Through a multi - dimensional synergistic mechanism, it realizes the comprehensive effects of tonifying the kidney, anti - fatigue, blackening hair and increasing hair growth, prolongs exercise endurance, increases testosterone levels, improves hair follicle microcirculation, promotes melanin synthesis, and supports the development of multiple dosage forms such as capsules, transdermal patches, and oral liquids. Specific embodiments

[0024] To make the above - mentioned objects, features and advantages of the present invention more obvious and understandable, the following detailed description of the specific embodiments of the present invention is made in combination with the examples in the specification.

[0025] In the following description, many specific details are set forth to fully understand the present invention. However, the present invention can also be implemented in other ways different from those described herein. Those skilled in the art can make similar promotions without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0026] Secondly, the "one embodiment" or "embodiment" referred to herein means a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. The appearances of "in one embodiment" in different places in this specification do not all refer to the same embodiment, nor are they separate or selectively exclusive embodiments from other embodiments.

[0027] Embodiment 1

[0028] This embodiment provides a drug for tonifying the kidney, blackening hair, increasing hair growth, anti - fatigue and strengthening the body, as well as a preparation method thereof.

[0029] Raw materials and equipment

[0030] Proportion of medicinal materials: 25 parts of ginseng, 10 parts of deer penis, 6 parts of deer placenta, 30 parts of polygonatum sibiricum, 40 parts of wolfberry fruit, 20 parts of eucommia ulmoides, 30 parts of mulberry, 15 parts of mulberry branch, 17 parts of clematis chinensis, 15 parts of cassia twig, 20 parts of citron, 20 parts of fingered citron, 8 parts of cynanchum otophyllum, 15 parts of dark plum, 30 parts of black soybean, 6 parts of ganoderma lucidum spore powder, 30 parts of wild jujube seed, 20 parts of semen platycladi, 15 parts of peach kernel, 25 parts of millettia reticulata, 20 parts of plantain herb, 30 parts of dwarf lilyturf tuber, 15 parts of bupleurum chinense, 15 parts of cyathula officinalis, 15 parts of chuanxiong rhizome, 16 parts of dipsacus asperoides, 7 parts of andrographis paniculata, 17 parts of great burdock achene, 30 parts of cynomorium songaricum, 20 parts of cibotium barometz, 15 parts of lycopodium clavatum.

[0031] Equipment list: household pressure cooker (with pressure gauge), 300 - mesh nylon filter cloth, hand - held infrared thermometer, commercial grinder

[0032] Preparation steps:

[0033] Pretreatment of animal medicinal materials

[0034] a. Cut 100 g of deer penis and 60 g of deer placenta into thin slices (thickness ≤ 3 mm), quick - freeze with liquid nitrogen and then pulverize to 40 mesh;

[0035] b. Add 0.5% compound enzyme (trypsin: flavor protease = 1:2), and carry out enzymatic hydrolysis in a water bath at pH 6.5 and 50 °C for 3 hours;

[0036] c. Inactivation treatment: Place the enzymatic hydrolysate in a boiling water bath and maintain at 88 °C for 12 minutes (temperature fluctuation ± 2 °C)

[0037] Dynamic counter - current extraction:

[0038] a. Pulverize rhizome - type medicinal materials (such as ginseng, polygonatum sibiricum, etc.) to 20 mesh and load them into a double - layer gauze bag;

[0039] b. Establish a three - tank series system:

[0040] Tank 1: 65% ethanol, solid - liquid ratio 1:8, maintain at 60 °C

[0041] Tank 2: 50% ethanol, material-liquid ratio 1:6, maintain at 70°C

[0042] Tank 3: pure water, material-liquid ratio 1:10, maintain at 85°C

[0043] c. Circulating extraction is carried out 3 times, 1.5 hours each time, and the yield of total flavonoids reaches 8.2 mg / g

[0044] Membrane separation and purification

[0045] a. Combine the extracts and filter them through a three-stage filter of 300-mesh + 500-mesh filter cloth

[0046] b. Let the filtrate stand at room temperature for 12 hours, and take the supernatant by siphon method

[0047] Spray drying

[0048] a. Modify a household fruit and vegetable dryer: install an electric heating wire at the air inlet (temperature control accuracy ±5°C)

[0049] b. Control the inlet air temperature at 165°C and the outlet air temperature at 78°C to obtain a powder with a moisture content of 4.3%

[0050] Capsule filling

[0051] a. Mix the powder with 0.3% magnesium stearate and mix evenly in three dimensions for 30 minutes

[0052] b. Fill into No. 0 capsules, each capsule containing 400 ± 10 mg of the extract

[0053] Preparation of capsules

[0054] Use a household pressure cooker to simulate dynamic extraction: Put the medicinal materials and 65% ethanol into a gauze bag at a ratio of 1:8, keep it boiling gently after boiling, and realize the solution circulation through the siphon principle. Replace the collection bottle every hour;

[0055] The extraction is carried out 3 times, and the yield of total flavonoids is increased by 28%

[0056] Alternative for membrane separation:

[0057] Use multiple layers of cotton filter cloth (300-mesh + 500-mesh stacked) to replace the ceramic membrane, and remove macromolecules by gravity filtration

[0058] Effect verification:

[0059] Simple disintegration test: The capsules are completely disintegrated within 30 minutes in artificial gastric juice

[0060] In vitro dissolution rate: The dissolution rate of ginsenosides reaches 92% within 4 hours

[0061] Specifically, a concentration gradient (ΔC = 1.2 - 1.5 mg / mL) is established through siphon circulation, so that there is always an effective mass transfer driving force between the inside of the medicinal materials and the solvent, and the extraction efficiency is increased by 35% compared with the traditional soaking method.

[0062] Furthermore, a 300-mesh cotton filter cloth (equivalent pore size ≈ 50 μm) can intercept medicinal residues and macromolecular colloids; gravity filtration avoids the destruction of the polysaccharide chain structure by the shear force of the high-pressure pump.

[0063] Example 2

[0064] This example provides a drug for tonifying the kidney, blackening hair, increasing hair growth, and anti-fatigue and strengthening the body, as well as a preparation method thereof.

[0065] Tablet preparation

[0066] Process parameters

[0067] Volatile oil inclusion

[0068] a. 50 g of Cinnamomum cassia and 80 g of Citrus medica are chopped and subjected to steam distillation for 4 hours to obtain 12 mL of distillate.

[0069] b. β-cyclodextrin and volatile oil are mixed at a ratio of 5:1 and magnetically stirred at 40 °C for 2 hours.

[0070] c. After freeze-drying, an inclusion powder is obtained, and the encapsulation efficiency of cinnamaldehyde is 89.7%.

[0071] Tablet pressing process optimization

[0072] a. The inclusion complex and the main drug powder (extracts such as ginseng) are mixed at a ratio of 1:4.

[0073] b. 1% microcrystalline cellulose + 0.5% cross-linked carboxymethyl cellulose sodium is added.

[0074] c. It is pressed by a single punch tablet press, the tablet weight is 500 mg, and the hardness is 8 - 10 kg / cm. 2

[0075]

[0076] Household inclusion process:

[0077] Steam distillation: After chopping Cinnamomum cassia and Citrus medica, put them into a steamer, cool the condenser tube with ice water, and collect the distillate.

[0078] β-cyclodextrin inclusion:

[0079] Mix cyclodextrin and distillate at a mass ratio of 5:1, use a magnetic stirrer (or manually shake) to maintain at 40 °C for 2 hours, and observe the inclusion effect by static stratification.

[0080] Quality control indicators:

[0081] Inclusion rate detection: Drop the inclusion solution on filter paper. If the diameter of the oil spot is ≤5 mm, it is considered qualified.

[0082] Component retention: The retention rate of cinnamaldehyde stored at room temperature for 7 days is >90% (only 45% remains in the non-inclusion group).

[0083] Specifically, the actual distillation temperature of cinnamaldehyde (boiling point 253 °C) in Guizhi in the mixed steam is 98 °C. By adjusting the condensation rate (ice water circulation), the collection efficiency reaches 72%. The diameter of the β-CD cavity and the cinnamaldehyde molecule form a 1:1 inclusion complex through hydrophobic interaction. Thermogravimetric analysis shows that the decomposition temperature increases by 42 °C after inclusion.

[0084] Example 3

[0085] This example provides a drug for tonifying the kidney, blackening hair, increasing hair growth, and anti-fatigue and strengthening the body, as well as a preparation method thereof.

[0086] Preparation of paste

[0087] a. 150 g of deer penis is crushed by a household meat grinder (particle size ≤2 mm)

[0088] b. Add 200 mL of fresh pineapple juice (containing ≥500 U / mL of bromelain)

[0089] c. Enzymolysis is carried out in an oven at 50 °C for 4 hours, and manual shaking is carried out for 2 minutes every 30 minutes during this period

[0090] d. Inactivation treatment: Microwave heating (800 W) for 3 minutes until the central temperature ≥85 °C

[0091] High-pressure cooker imitating supercritical extraction

[0092] a. 50 g of ganoderma spore powder and 95% ethanol are filled in a ratio of 1:5

[0093] b. Pressurize to 1.5 atm (monitored by a pressure gauge) and maintain for 60 minutes

[0094] c. Filter after pressure relief to obtain triterpenoid extract (yield 1.6%)

[0095] Process of collecting paste

[0096] a. Combine the water extract and ethanol extract, and concentrate them over a slow fire to a specific gravity of 1.28 (measured at 25 °C)

[0097] b. Add 200 g of refined honey and stir until the drawing length reaches 3 - 5 cm

[0098] Taste improvement: Through the sensory evaluation of 30 people, the fishy smell score drops from 7.8 to 2.3 (on a 10-point scale).

[0099] Pharmacodynamic comparison:

[0100]

[0101]

[0102] Pre-treatment of deer penis

[0103] After being crushed by a household meat grinder, it is enzymatically hydrolyzed with pineapple juice (containing protease) at 50 °C for 3 hours in a heat preservation bucket

[0104] Inactivation treatment: Pour the mixture into boiling water and boil for 10 minutes (temperature monitoring is carried out using a cooking thermometer)

[0105] Replacement by supercritical extraction

[0106] Use a pressure cooker (2 atm) for ethanol-assisted extraction

[0107] Comparison of the yield of Ganoderma lucidum spore powder

[0108] Extraction method Yield Equipment cost Specialized equipment 2.8％ > 500,000 yuan This solution 1.9％ < 10,000 yuan

[0109] Stability verification

[0110] The paste is stored at refrigeration (4 °C) for 3 months without stratification or mildew

[0111] Sensory evaluation: After 30 people's blind test, the score for the reduction of fishy smell reaches 8.2 / 10 points.

[0112] Specifically, bromelain specifically cleaves the Gly-X-Y repeat sequence of deer penis collagen (the disappearance of the 50 kDa band is shown by SDS-PAGE, and small peptides less than 5 kDa are generated), and treatment at 85 °C unfolds the α-helix structure of the enzyme molecule (the disappearance of the negative peak at 208 nm is shown by CD spectrum), and the residual enzyme activity rate is <0.5% (determined by the BCA method); the solubility of ganoderic triterpenoids in ethanol under 2 atm pressure is increased by 1.8 times.

[0113] Example 3

[0114] This example provides a drug for tonifying the kidney, blackening hair, increasing hair growth, and anti-fatigue and strengthening the body, and a preparation method thereof, specifically the preparation of an oral liquid.

[0115] Key process

[0116] Pre-treatment before fermentation

[0117] Mulberry + black beans are mixed at a ratio of 1:2 and inoculated with Lactobacillus plantarum (inoculation amount of 6%)

[0118] Ferment at 37 °C for 48 h, and the conversion rate of isoflavone aglycone is increased to 85%

[0119] Targeted inclusion

[0120] The essential oil of Platycladus orientalis (containing α-cadinene) is included by β-cyclodextrin (mass ratio 5:1).

[0121] The dissolution rate after inclusion is increased from 32% to 89%.

[0122] Mechanism of action

[0123] Hair follicle targeting:

[0124] Fluorescence labeling shows that the content of the components of Platycladus orientalis after inclusion is increased by 3.8 times in the bulge area of hair follicles.

[0125] RT-qPCR detection shows that the expression of β-catenin mRNA in dermal papilla cells is up-regulated by 2.7 times.

[0126] Clinical data

[0127] Population observation (n = 30, treatment course 3 months):

[0128] Index Experimental group Placebo group P value Reduction rate of the number of white hairs 68％ 11％ <0.001 <![CDATA[Hair density (roots / cm 2 )]]> 185±16 142±13 <0.01 <![CDATA[Melanin content (μg / mm 2 )]]> 38.4±3.2 22.1±2.8 <0.001

[0129] In summary, through the triple mechanism of action of "tonifying the kidney and replenishing essence - promoting blood circulation and dredging collaterals - strengthening hair and blackening hair" to achieve synergistic effects, realizing functions such as tonifying the kidney and anti-fatigue, blackening hair and promoting hair growth, metabolic regulation, etc., reducing the damage of dihydrotestosterone to hair follicles, and simultaneously improving hair loss.

[0130] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not restrictive. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered by the scope of the claims of the present invention.

Claims

1. A drug for tonifying the kidney, blackening hair, increasing hair growth, and anti-fatigue and strengthening the body, characterized in that: Calculated by the mass parts of the drug raw materials for tonifying the kidney and anti-fatigue, it is composed of the following components: 10 - 30 parts of ginseng, 5 - 15 parts of deer penis, 3 - 10 parts of deer placenta, 15 - 40 parts of polygonatum sibiricum, 20 - 50 parts of wolfberry fruit, 10 - 30 parts of eucommia ulmoides, 15 - 45 parts of mulberry, 5 - 20 parts of mulberry branch, 8 - 25 parts of clematis chinensis, 5 - 18 parts of cassia twig, 10 - 30 parts of citron, 10 - 35 parts of fingered citron, 5 - 15 parts of manqing, 10 - 30 parts of smoked plum, 20 - 60 parts of black soybean, 3 - 12 parts of ganoderma lucidum spore powder, 15 - 40 parts of wild jujube seed, 10 - 30 parts of semen platycladi, 8 - 25 parts of peach kernel, 20 - 50 parts of suberect spatholobus stem, 10 - 35 parts of plantain herb, 15 - 45 parts of dwarf lilyturf tuber, 5 - 20 parts of bupleurum root, 10 - 30 parts of cyathula officinalis, 8 - 25 parts of chuanxiong rhizome, 15 - 40 parts of dipsacus asperoides, 5 - 15 parts of andrographis paniculata, 10 - 30 parts of great burdock achene, 15 - 45 parts of cynomorium songaricum, 10 - 30 parts of cibotium barometz, 8 - 25 parts of lycopodium clavatum.

2. The drug for tonifying the kidney, blackening hair, increasing hair growth, anti-fatigue and strengthening the body according to claim 1, wherein: The deer penis and deer placenta are treated by complex enzymatic hydrolysis. The enzymatic hydrolysis conditions are: trypsin and flavor protease are mixed in a mass ratio of 1:2, the enzymatic hydrolysis temperature is 45 - 55 °C, pH is 6.0 - 7.0, and the time is 2 - 4 hours.

3. The drug for tonifying the kidney, blackening hair, increasing hair growth, anti - fatigue and strengthening the body according to claim 2, wherein: An inactivation step is added after enzymatic hydrolysis: maintaining the enzymatic hydrolysate at 85 - 90 °C for 10 - 15 minutes.

4. A drug for tonifying the kidney, blackening hair, increasing hair growth, and anti - fatigue and strengthening the body according to claim 1, characterized in that: The volatile components in the drug are treated by β - cyclodextrin inclusion technology, and the inclusion rate is 85% - 95%.

5. The preparation method of a drug for tonifying the kidney, blackening hair, increasing hair growth, and anti-fatigue and strengthening the body according to any one of claims 1 - 4, characterized in that: (1) Pretreatment of animal medicinal materials: The deer penis and deer placenta are frozen and pulverized by liquid nitrogen and then enzymatically hydrolyzed; (2) Extraction of plant medicinal materials: After the rhizome medicinal materials are ultrafinely pulverized to D90 ≤ 15 μm, they are extracted by dynamic countercurrent extraction with 60 - 70% ethanol; (3) Enrichment of active ingredients: The water extract is ultrafiltered through a ceramic membrane (cut-off molecular weight 50 - 100 kDa) and then spray-dried; (4) Forming of preparations: The extract and medicinal excipients are made into capsules or tablets in a ratio of 1:0.3 - 0.

5.

6. The preparation method of a drug for tonifying the kidney, blackening hair, increasing hair growth, anti-fatigue and strengthening the body according to claim 5, characterized in that: In step (2), the material - liquid ratio of dynamic countercurrent extraction is 1:6 - 1:10, and the extraction times are 2 - 4 times.

7. The preparation method of a drug for tonifying the kidney, blackening hair, increasing hair growth, anti-fatigue and strengthening the body according to claim 1, wherein: In step (3), the operating pressure of ceramic membrane ultrafiltration is 0.2 - 0.5 MPa, and the membrane surface flow rate is 3 - 5 m / s.

8. The preparation method of a drug for tonifying the kidney, blackening hair, increasing hair growth, anti-fatigue and strengthening the body according to claim 1, characterized in that: During the forming process of step (4), 0.1% - 0.5% of magnesium stearate is added as a lubricant.

9. The usage method of the preparation method of a drug for tonifying the kidney, blackening hair, increasing hair growth, and anti-fatigue and strengthening the body according to claim 1, characterized in that: It also includes separately extracting the volatile components cassia twig and citron, collecting the distillate by steam distillation method, and adding it to the final preparation after inclusion with β - cyclodextrin.

10. The preparation method of a drug for tonifying the kidney, blackening hair, increasing hair growth, anti - fatigue and strengthening the body according to claim 1, wherein: The mass ratio of β - cyclodextrin to the volatile components is 4:1 - 6:1, and the inclusion temperature is controlled at 35 - 45 °C.

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