A D-lactic acid content determination kit and determination method

Abstract

The present invention discloses a D-lactic acid content determination kit and determination method, which relate to the technical field of D-lactic acid content determination, including a detection box body, wherein the side of the detection box body is provided with an assembly strip for assembly, and the top of the detection box body is provided with a protective cover, and the outermost side of the detection box body on the end side is provided with a mixing cylinder for mixing reagents, the interior of the detection box body is fixedly connected to an auxiliary base, and the upper side of the auxiliary base is provided with a clamping plate, and the interior of the auxiliary base is provided with an adjustment mechanism. In this D-lactic acid content determination kit and determination method, D-lactic acid generates pyruvate under the action of D-lactate dehydrogenase, and reduces NAD+ to generate NADH; in order to make the reaction proceed smoothly, another enzyme is added to further decompose the pyruvate, and the generated NADH reacts with a specific color developer to produce a colored substance with a maximum absorption peak at 450nm. The D-lactic acid content is calculated by detecting the increase in the substance at 450nm.

Description

Technical Field

[0001] The present invention relates to the technical field of D-lactic acid content determination, in particular to a D-lactic acid content determination kit and a determination method. Background Art

[0002] D-lactic acid is an optical isomer of lactic acid. The finished product is a colorless or light yellow viscous liquid with a slightly sour taste. It is easily soluble in water, ethanol, and ether, but insoluble in chloroform. The human body can only slowly metabolize D-lactic acid through specific enzymes (such as D-2-hydroxyacid dehydrogenase). Excessive intake may lead to metabolic acidosis. Therefore, the detection of D-lactic acid content is of great value in medical diagnosis (such as metabolic abnormalities, acidosis), industrial production (such as biodegradable materials), and scientific research (such as chiral synthesis).

[0003] The prior art (Chinese patent publication number CN101324555A, publication date December 17, 2008) discloses a D-lactic acid diagnosis / determination kit utilizing an enzymatic colorimetric method specific for D-lactic acid. The present invention also relates to the method principle, reagent composition, and ingredients for determining D-lactic acid concentration, belonging to the field of medical / food inspection and determination technology. The kit primarily comprises a buffer, a coenzyme, D-lactate dehydrogenase, and a stabilizer. The kit is prepared by mixing a sample and the reagent in a specific volume ratio to induce a series of enzymatic reactions. The reactants are then placed under an ultraviolet / visible light analyzer and the degree / rate of absorbance increase at a dominant wavelength of 340 nm is detected, thereby calculating the D-lactic acid concentration.

[0004] Although the existing D-lactic acid assay kit can perform the assay, the box body cannot provide stable protection for the reagent tube when it is placed. At the same time, different reagents require different storage temperatures. When the temperature inside the kit drops, the reagents may become ineffective, which has certain limitations.

[0005] Therefore, we proposed a D-lactic acid content determination kit and determination method to solve the above problems. Summary of the Invention

[0006] The object of the present invention is to provide a D-lactic acid content determination kit and determination method to solve the problems raised in the above-mentioned background technology, namely that the box bodies currently on the market cannot stably protect the reagent tubes when placing them, and different reagents require different storage temperatures. When the temperature inside the kit drops, the reagents may become ineffective, which has certain limitations.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: a D-lactic acid content determination kit and determination method, comprising a detection box body, an assembly strip for assembly is provided on the side of the detection box body, a protective cover is provided on the top of the detection box body, and a mixing cylinder for mixing reagents is provided on the outermost side of the detection box body on the end side, an auxiliary base is fixedly connected to the interior of the detection box body, and a clamping plate is provided on the upper side of the auxiliary base, an adjustment mechanism is provided inside the auxiliary base, and the adjustment mechanism realizes the movement and clamping operation of the clamping plate through gas exchange between the first airbag and the second airbag contained therein, a limit frame is fixedly connected to the inner wall of the detection box body, and a feedback mechanism is provided inside the limit frame, and the feedback mechanism realizes feedback on the internal temperature of the detection box body through the position state change of the feedback rod contained therein.

[0008] Preferably, a docking rod is fixedly connected to the lower surface of the protective cover, a docking groove adapted to the docking rod is provided on the upper surface of the detection box body, and an auxiliary box is fixedly connected below the docking groove.

[0009] Preferably, the adjustment mechanism includes a movable groove, which is opened inside the auxiliary base, and a sliding block is nested and connected inside the movable groove, and the upper surface of the sliding block is fixedly connected to the lower surface of the clamping plate, and a second airbag is fixedly connected between the side of the sliding block and the side wall of the movable groove, the first airbag is fixedly connected inside the auxiliary box, and a connecting hose is provided between the second airbag and the first airbag.

[0010] Preferably, a rotating shaft is rotatably provided at the center of the clamping plate, and the middle part of the outer side of the rotating shaft is fixedly connected to the limiting plate, and a torsion spring is fixedly connected between the upper end side of the limiting plate and the clamping plate, a traction rope is wrapped around the outer side of the rotating shaft, and the other end of the traction rope is fixedly connected to the upper surface of the auxiliary base.

[0011] Preferably, the sliding block forms a sliding structure through the second airbag and the movable groove, the limiting plate forms a rotating structure through the rotating shaft and the clamping plate, and rubber points are provided on the inner side of the limiting plate.

[0012] Preferably, an auxiliary shaft is rotatably connected to the center of the movable groove, a turntable is fixedly connected to the outside of the auxiliary shaft, and a rotating rod is hinged on the side of the turntable, a pull rod is fixedly connected to the side of the sliding block, and the inner end of the pull rod is hinged to the other side of the rotating rod.

[0013] Preferably, a button is fixedly connected to the inside of the movable groove, a connecting rod is fixedly connected to the side of the auxiliary shaft, and the outer end of the connecting rod is fixedly connected to the first magnetic block, the internal nesting connection of the auxiliary base is a limiting rod, and the end of the limiting rod close to the first magnetic block is fixedly connected to the second magnetic block, and the end of the second magnetic block close to the button is fixedly connected to a pressure plate, a second spring is fixedly connected between the inner side of the pressure plate and the movable groove, and a temperature control tube electrically connected to the button is provided on the upper surface of the auxiliary base.

[0014] Preferably, the feedback mechanism includes a heat conducting plate, which is nested and connected to the side of the limit frame, and the interior of the limit frame is nested and connected with a memory alloy, and the side of the memory alloy is fixedly connected to the inner end of the heat conducting plate, and the front side of the detection box body is nested and connected with a contact plate corresponding to the position of the limit frame, and the contact plate is fixedly connected to the inner end of the feedback rod, and the feedback rod is nested on the detection box body.

[0015] A method for determining the content of D-lactic acid in a kit for determining the content of D-lactic acid, applied to the kit for determining the content of D-lactic acid as claimed in claim 1, comprising the following steps:

[0016] S1: After placing the reagent inside the reagent tube, the reagent tube is placed inside the corresponding detection box body. At this time, the protective cover and the detection box body are closed, the second airbag will expand and push the sliding block, and the moving sliding block will drive the clamping plates on both sides to slide towards each other and preliminarily clamp the reagent tube. At the same time, the rotating shaft rotates under the traction of the traction rope, and drives the limit plate to rotate inward synchronously, thereby further resisting the reagent tube, preventing the reagent tube from deviating and shaking, and avoiding damage to the reagent tube;

[0017] S2: When the sliding block moves, it pushes the rotating rod hinged to it synchronously through the pull rod. At this time, the turntable synchronously drives the rotating shaft to rotate. The rotating shaft can drive the first magnetic block to rotate through the connecting rod. At this time, the first magnetic block does not adsorb the second magnetic block. The pressure plate can slide to one side of the button driven by the elastic force of the second spring, thereby pressing the button to start the temperature control tube and perform temperature control. When the temperature control of the temperature control tube fails, the memory alloy will sense the temperature change and bend and deform. The bent memory alloy can resist the resistance plate and drive the feedback rod to move outward. The user can observe the position of the feedback rod to know whether the inside of the test box is cold and check the status of the reagent.

[0018] S3: D-lactic acid generates pyruvate under the action of D-lactate dehydrogenase, and reduces NAD+ to generate NADH. In order to make the reaction proceed smoothly, another enzyme is added to further decompose pyruvate, generating NADH, which reacts with a specific color developer to produce a colored substance with a maximum absorption peak at 450nm. The D-lactic acid content is calculated by detecting the increase in the substance at 450nm.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] When the protective cover and the detection box body are closed, the first airbag is squeezed by the docking rod set under the protective cover. At this time, the gas inside the first airbag is transmitted to the inside of the second airbag through the connecting hose, thereby realizing gas exchange between the first airbag and the second airbag. When the second airbag expands, it will drive the clamping plate to move toward each other through the sliding block, thereby assisting in clamping and limiting the reagent tube.

[0021] During the movement of the clamping plate, the rotating shaft can be pulled by the traction action of the traction rope, so that the rotating shaft drives the limit plate to rotate inward, and the outward-extended limit plate further conflicts with the reagent tube, and the friction between the limit plate and the reagent tube is increased by the rubber points set on the side, so that the reagent tube can be placed more stably inside the detection box.

[0022] During the movement of the sliding block, the turntable can be driven to rotate through the cooperation between the pull rod and the rotating rod. At this time, the rotating shaft can synchronously cause the first magnetic block and the second magnetic block to be misaligned under the drive of the turntable, so that the pressure plate presses the button. At this time, the temperature control tube starts to run, so that the temperature inside the detection box can be adjusted according to the needs of different reagents.

[0023] A feedback rod is provided on the body of the detection box. When the temperature inside the detection box is in a constant state, the position of the feedback rod will not change. When the temperature control tube fails to operate normally, causing the detection box to lose temperature, the feedback rod will move outward, thereby providing feedback on the temperature inside the detection box.

[0024] The internal temperature of the detection box can be transmitted to the memory alloy through the heat conduction plate. When the temperature changes, the memory alloy will deform, thereby squeezing the contact plate and causing the feedback rod to move outward. At this time, the user can observe the position and distance of the feedback rod to determine whether the detection box is damaged. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0026] Figure 2 This is a schematic diagram of the three-dimensional cross-sectional structure of the detection box body of the present invention;

[0027] Figure 3This is a schematic diagram of the three-dimensional cross-sectional structure of the auxiliary box of the present invention;

[0028] Figure 4 This is a schematic diagram of the three-dimensional structure of the connecting hose of the present invention;

[0029] Figure 5 This is a schematic diagram of the three-dimensional structure of the clamping plate of the present invention;

[0030] Figure 6 This is a schematic diagram of a three-dimensional cross-sectional structure of the clamping plate of the present invention;

[0031] Figure 7 This is a schematic diagram of a three-dimensional cross-sectional structure of the auxiliary base of the present invention;

[0032] Figure 8 This is a schematic diagram of the three-dimensional structure of the pressing plate of the present invention;

[0033] Figure 9 This is a schematic diagram of the three-dimensional structure of the limiting frame of the present invention;

[0034] Figure 10 It is a schematic diagram of the three-dimensional cross-sectional structure of the limit frame of the present invention.

[0035] In the figure: 1. Detection box body; 2. Protective cover; 3. Assembly bar; 4. Mixing barrel; 5. Docking slot; 6. Auxiliary box; 7. First airbag; 8. Second airbag; 9. Connecting hose; 10. Docking rod; 11. Auxiliary base; 12. Movable slot; 13. Auxiliary shaft; 14. Rotating shaft; 15. Limiting plate; 16. Clamping plate; 17. Sliding block; 18. Pull rope; 19. Button; 20. First magnetic block; 21. Second magnetic block; 22. Connecting rod; 23. Rotating rod; 24. Limiting rod; 25. Torsion spring; 26. Temperature control tube; 27. Turntable; 28. Pressing plate; 29. Second spring; 30. Limiting frame; 31. Heat conduction plate; 32. Memory alloy; 33. Feedback rod; 34. Contact plate; 35. Pull rod. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] Example 1: Figure 1-Figure 4The technical solution shown in the figure, the present invention provides the following technical solutions: a D-lactic acid content determination kit and determination method, discloses an adjustment mechanism, through which the reagent tube can be clamped and limited to prevent the reagent tube from shifting and shaking: an assembly strip 3 for assembly is provided on the side of the detection box body 1, and a protective cover 2 is provided on the upper part of the detection box body 1, and a mixing cylinder 4 for mixing the reagent is provided on the outermost side of the detection box body 1 on the end side, an auxiliary base 11 is fixedly connected to the interior of the detection box body 1, and a clamping plate 16 is provided on the upper side of the auxiliary base 11, an adjustment mechanism is provided inside the auxiliary base 11, and the adjustment mechanism realizes the moving clamping operation of the clamping plate 16 through the gas exchange between the first air bag 7 and the second air bag 8 contained therein, a docking rod 10 is fixedly connected to the lower surface of the protective cover 2, a docking groove 5 adapted to the docking rod 10 is provided on the upper surface of the detection box body 1, and an auxiliary box 6 is fixedly connected below the docking groove 5, and the adjustment mechanism includes a movable groove 1 2. The movable groove 12 is opened inside the auxiliary base 11, and the movable groove 12 is nested with a sliding block 17, and the upper surface of the sliding block 17 is fixedly connected to the lower surface of the clamping plate 16, and the side of the sliding block 17 is fixedly connected to the side wall of the movable groove 12 with the second airbag 8. The first airbag 7 is fixedly connected to the inside of the auxiliary box 6, and a connecting hose 9 is provided between the second airbag 8 and the first airbag 7. A rotating shaft 14 is provided at the center of the clamping plate 16 for rotation. , and the middle part of the outer side of the rotating shaft 14 is fixedly connected to the limiting plate 15, and a torsion spring 25 is fixedly connected between the upper end side of the limiting plate 15 and the clamping plate 16, a traction rope 18 is wrapped around the outer side of the rotating shaft 14, and the other end of the traction rope 18 is fixedly connected to the upper surface of the auxiliary base 11, the sliding block 17 forms a sliding structure with the movable groove 12 through the second airbag 8, the limiting plate 15 forms a rotating structure through the rotating shaft 14 and the clamping plate 16, and a rubber point is provided on the inner side of the limiting plate 15.

[0038] After placing the reagent inside the reagent tube, place the reagent tube inside the corresponding detection box body 1. At this time, the docking rod 10 set at the lower end of the protective cover 2 is aligned with the docking groove 5, so as to close the protective cover 2 and the detection box body 1. At this time, the docking rod 10 is docked to the inside of the auxiliary box 6, thereby squeezing the first airbag 7. When the first airbag 7 is squeezed, the internal gas will be transmitted to the inside of the second airbag 8 through the connecting hose 9, so that the second airbag 8 will expand and push the sliding block 17, so that the sliding block 17 drives the clamping plates 16 on both sides to slide toward each other and preliminarily clamp the reagent tube. When the clamping plate 16 is moving, the end is fixed by the traction rope 18, which will pull the rotating shaft 14 at this time, so that the rotating shaft 14 rotates. The rotating rotating shaft 14 can drive the limit plate 15 to rotate synchronously inward, thereby further resisting the reagent tube, preventing the reagent tube from offset and shaking, and avoiding damage to the reagent tube.

[0039] Example 2: Figure 3-Figure 7 The technical solution shown in the figure, the present invention provides the following technical solutions: a D-lactic acid content determination kit and determination method, discloses a temperature control tube 26, which can be synchronously started by the driving action of the adjustment mechanism to perform temperature control on the interior of the detection box body 1: the center of the movable groove 12 is rotatably connected to the auxiliary shaft 13, the outer side of the auxiliary shaft 13 is fixedly connected to the turntable 27, and the side of the turntable 27 is hinged with a rotating rod 23, the side of the sliding block 17 is fixedly connected to a pull rod 35, and the inner end of the pull rod 35 is hinged to the other side of the rotating rod 23, and the movable groove 12 is rotatably connected to the auxiliary shaft 13, and the outer side of the auxiliary shaft 13 is fixedly connected to the turntable 27, and the side of the turntable 27 is hinged with a rotating rod 23, and the side of the sliding block 17 is fixedly connected to the pull rod 35, and the inner end of the pull rod 35 is hinged to the other side of the rotating rod 23. A button 19 is fixedly connected to the inside of the groove 12, a connecting rod 22 is fixedly connected to the side of the auxiliary shaft 13, and the outer end of the connecting rod 22 is fixedly connected to the first magnetic block 20, and a limiting rod 24 is nested inside the auxiliary base 11, and the end of the limiting rod 24 close to the first magnetic block 20 is fixedly connected to the second magnetic block 21, and the end of the second magnetic block 21 close to the button 19 is fixedly connected to a pressure plate 28, and a second spring 29 is fixedly connected between the inner side of the pressure plate 28 and the movable groove 12, and a temperature control tube 26 electrically connected to the button 19 is provided on the upper surface of the auxiliary base 11.

[0040] During the movement of the sliding block 17, it can synchronously drive the pull rod 35 to move, thereby synchronously pushing the rotating rod 23 hinged to it through the pull rod 35. At this time, the rotating rod 23 can drive the turntable 27 to rotate. When the turntable 27 is rotating, it can synchronously drive the rotating shaft 14 to rotate. The rotating shaft 14 can drive the first magnetic block 20 to rotate through the connecting rod 22. At this time, the first magnetic block 20 does not adsorb the second magnetic block 21, and the second magnetic block 21 does not attract the limit rod 24. The pressure plate 28 can slide to one side of the button 19 under the drive of the elastic force of the second spring 29, thereby pressing the button 19 to start the temperature control tube 26 for temperature control.

[0041] Example 3: Figure 3 、 Figure 9 and Figure 10The technical solution shown in the figure, the present invention provides the following technical solution: a D-lactic acid content determination kit and determination method, disclosing a feedback mechanism, through which the temperature state inside the detection box body 1 can be fed back: a limit frame 30 is fixedly connected to the inner wall of the detection box body 1, and a feedback mechanism is provided inside the limit frame 30, and the feedback mechanism realizes feedback on the internal temperature of the detection box body 1 through the position state change of the feedback rod 33 contained therein, and the feedback mechanism includes a heat conducting plate 31, which is nested and connected to the side of the limit frame 30, and the inside of the limit frame 30 is nested and connected with a memory alloy 32, and the side of the memory alloy 32 is fixedly connected to the inner end of the heat conducting plate 31, and the front side of the detection box body 1 is nested and connected with a contact plate 34 corresponding to the position of the limit frame 30, and the contact plate 34 is fixedly connected to the inner end of the feedback rod 33, and the feedback rod 33 is nested on the detection box body 1.

[0042] When the temperature control tube 26 fails to control the temperature, the heat conducting plate 31 transmits the temperature inside the detection box body 1 to the memory alloy 32. The memory alloy 32 will sense the temperature change and bend. The bent memory alloy 32 can contact the contact plate 34, and the contact plate 34 drives the feedback rod 33 to move outward. The user can observe the position of the feedback rod 33 to know whether the inside of the detection box body 1 is losing temperature and check the status of the reagent.

[0043] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A D-lactic acid content determination kit, comprising a detection box body (1), wherein an assembly strip (3) for assembly is provided on the side of the detection box body (1), a protective cover (2) is provided above the detection box body (1), and a mixing cylinder (4) for mixing reagents is provided on the outermost side of the detection box body (1) at the end side, characterized in that: The interior of the detection box body (1) is fixedly connected to an auxiliary base (11), and a clamping plate (16) is provided on the upper side of the auxiliary base (11). An adjustment mechanism is provided inside the auxiliary base (11), and the adjustment mechanism realizes the movement and clamping operation of the clamping plate (16) through gas exchange between the first airbag (7) and the second airbag (8) contained therein. A limit frame (30) is fixedly connected to the inner wall of the detection box body (1), and a feedback mechanism is provided inside the limit frame (30). The feedback mechanism realizes feedback of the internal temperature of the detection box body (1) through the position state change of the feedback rod (33) contained therein; The lower surface of the protective cover (2) is fixedly connected to a docking rod (10), the upper surface of the detection box body (1) is provided with a docking groove (5) adapted to the docking rod (10), and an auxiliary box (6) is fixedly connected below the docking groove (5), and the adjustment mechanism includes a movable groove (12), the movable groove (12) is provided inside the auxiliary base (11), and a sliding block (17) is nested and connected inside the movable groove (12), and the upper surface of the sliding block (17) is fixedly connected to the lower surface of the clamping plate (16), and a second airbag (8) is fixedly connected between the side of the sliding block (17) and the side wall of the movable groove (12), the first airbag (7) is fixedly connected inside the auxiliary box (6), and the second airbag ( 8) and the first airbag (7) are provided with a connecting hose (9), a rotating shaft (14) is rotatably provided at the center of the clamping plate (16), and the middle part of the outer side of the rotating shaft (14) is fixedly connected to the limiting plate (15), and a torsion spring (25) is fixedly connected between the upper end side of the limiting plate (15) and the clamping plate (16), a traction rope (18) is wound around the outer side of the rotating shaft (14), and the other end of the traction rope (18) is fixedly connected to the upper surface of the auxiliary base (11), the sliding block (17) forms a sliding structure with the second airbag (8) and the movable groove (12), the limiting plate (15) forms a rotating structure with the clamping plate (16) through the rotating shaft (14), and a rubber point is provided on the inner side of the limiting plate (15).

2. A D-lactic acid content determination kit according to claim 1, characterized in that: An auxiliary shaft (13) is rotatably connected to the center of the movable groove (12), a turntable (27) is fixedly connected to the outside of the auxiliary shaft (13), and a rotating rod (23) is hinged to the side of the turntable (27), and a pull rod (35) is fixedly connected to the side of the sliding block (17), and the inner end of the pull rod (35) and the other side of the rotating rod (23) are hinged to each other.

3. A D-lactic acid content determination kit according to claim 2, characterized in that: The movable groove (12) is fixedly connected to a button (19), the side of the auxiliary shaft (13) is fixedly connected to a connecting rod (22), and the outer end of the connecting rod (22) is fixedly connected to a first magnetic block (20), the auxiliary base (11) is nested with a limiting rod (24), and the end of the limiting rod (24) close to the first magnetic block (20) is fixedly connected to a second magnetic block (21), and the end of the second magnetic block (21) close to the button (19) is fixedly connected to a pressure plate (28), and a second spring (29) is fixedly connected between the inner side of the pressure plate (28) and the movable groove (12), and a temperature control tube (26) electrically connected to the button (19) is provided on the upper surface of the auxiliary base (11).

4. A D-lactic acid content determination kit according to claim 3, characterized in that: The feedback mechanism includes a heat conducting plate (31), the heat conducting plate (31) is nested and connected to the side of the limit frame (30), and the inside of the limit frame (30) is nested and connected with a memory alloy (32), and the side of the memory alloy (32) is fixedly connected to the inner end of the heat conducting plate (31), the front side of the detection box body (1) is nested and connected with a contact plate (34) corresponding to the position of the limit frame (30), and the contact plate (34) is fixedly connected to the inner end of the feedback rod (33), and the feedback rod (33) is nested and arranged on the detection box body (1).

5. A method for determining the content of D-lactic acid using a kit for determining the content of D-lactic acid, applied to the kit for determining the content of D-lactic acid as claimed in claim 4, characterized in that: The steps include: S1: After placing the reagent inside the reagent tube, the reagent tube is placed inside the corresponding detection box body (1). At this time, the protective cover (2) and the detection box body (1) are closed, the second airbag (8) will expand and push the sliding block (17), and the moving sliding block (17) will drive the clamping plates (16) on both sides to slide towards each other and preliminarily clamp the reagent tube. At the same time, the rotating shaft (14) rotates under the traction of the traction rope (18), and drives the limit plate (15) to rotate synchronously inward, thereby further resisting the reagent tube, preventing the reagent tube from deviating and shaking, and avoiding damage to the reagent tube; S2: During the movement of the sliding block (17), the pull rod (35) pushes the rotating rod (23) hinged thereto. At this time, the turntable (27) drives the rotating shaft (14) to rotate synchronously. The rotating shaft (14) can drive the first magnetic block (20) to rotate through the connecting rod (22). At this time, the first magnetic block (20) does not adsorb the second magnetic block (21). The pressing plate (28) can slide toward one side of the button (19) under the elastic force of the second spring (29), thereby pressing the button (19) to start the temperature control tube (26) and perform temperature control. When the temperature control of the temperature control tube (26) fails, the memory alloy (32) will sense the temperature change and bend and deform. The bent memory alloy (32) can contact the contact plate (34) and drive the feedback rod (33) to move outward. The user can observe the position state of the feedback rod (33) to know whether the inside of the detection box (1) is cold and check the status of the reagent. S3: D-lactic acid generates pyruvate under the action of D-lactate dehydrogenase, and reduces NAD+ to NADH; in order to make the reaction proceed smoothly, another enzyme is added to further decompose pyruvate, generating NADH, which reacts with a specific color developer to produce a colored substance with a maximum absorption peak at 450nm. The D-lactic acid content is calculated by detecting the increase in the substance at 450nm.

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