Experimental device and method for animal turning conditioned reflex

Abstract

The invention belongs to the technical field of animal behavioral experimental equipment, and discloses an experimental device and method for animal turning conditioned reflex, and the experimental device comprises an experimental module, and a monitoring camera, a running wheel, a light stimulation device, a motion data acquisition device and a reward delivery device which are arranged in the experimental module; the system further comprises a control terminal. The control terminal is connected with the monitoring camera, the light stimulation device, the motion data acquisition device and the reward delivery device; wherein the control terminal is used for controlling the light stimulation device and the reward delivery device according to the operation data of the running wheel; the experimental device disclosed by the invention has multiple functions and automatic control capability, accurate data acquisition and automatic experimental condition adjustment are beneficial to improving the experimental quality and obtaining more accurate and more valuable experimental results, so that the experimental process is more efficient and convenient, and a wider experimental space is provided for animal behavioral research.

Description

Technical Field

[0001] The present invention belongs to the technical field of animal behavior experiment equipment, and particularly relates to an experimental device and method for animal wheel conditioning reflexes. Background Art

[0002] In the study of animal behavior, the running wheel experiment is one of the commonly used experimental means, which is used to study animal behavior, conditioned reflexes, and learning and memory; currently, the functions of existing running wheel experiment equipment are relatively single, and it is difficult to achieve accurate collection of motion data and control of conditioned reflexes; secondly, existing equipment usually relies on manual operation and cannot adjust experimental conditions according to motion data, resulting in large errors in the results of running wheel experiments. Summary of the Invention

[0003] In view of the technical problems existing in the prior art, the present invention provides an experimental device and method for animal wheel conditioning reflexes to solve the technical problems that existing running wheel experiment equipment is difficult to achieve accurate collection of motion data and control of conditioned reflexes, resulting in large errors in the results of running wheel experiments.

[0004] To achieve the above object, the technical solution adopted by the present invention is as follows: The present invention provides an experimental device for animal wheel conditioning reflexes, including an experimental chamber and a monitoring camera, a running wheel, a light stimulation device, a motion data collection device, and a reward delivery device arranged in the experimental chamber; The monitoring camera is used to collect video data of animal behavior in the experimental chamber; The light stimulation device is used to emit flashing light to train the conditioned reflexes of animals; The motion data collection device is used to collect the running data of the running wheel; The reward delivery device is used to deliver food rewards; It further includes a control terminal; the control terminal is connected to the monitoring camera, the light stimulation device, the motion data collection device, and the reward delivery device; wherein, the control terminal is used to control the light stimulation device and the reward delivery device according to the running data of the running wheel.

[0005] Furthermore, it further includes a host computer and a mobile terminal connected to the control terminal; The control terminal is used to send the video data of animal behavior in the experimental chamber, the running data of the running wheel, the running data of the light stimulation device, and the running data of the reward delivery device to the host computer and the mobile terminal; The host computer is used to analyze the animal behavior video data, the running data of the running wheel, the running data of the light stimulation device, and the running data of the reward delivery device in the experimental chamber, so as to obtain the relationship between the animal's activity frequency and light stimulation, running wheel movement, and reward intensity; The mobile terminal is used to query the animal behavior video data, the running data of the running wheel, the running data of the light stimulation device, and the running data of the reward delivery device in the experimental chamber.

[0006] Further, the control terminal includes a main control unit, and a voltage control module, a serial communication module, and a WiFi module that are all connected to the main control unit; The main control unit is used to output a light stimulation control signal and a reward delivery control signal according to the running data of the running wheel and based on a preset conditioned reflex rule; wherein, the light stimulation control signal is used to trigger the light stimulation device to emit flashing light; the reward delivery control signal is used to trigger the voltage control module to generate a start / stop instruction to the reward delivery device; The serial communication module is used for communication between the main control unit and the host computer; The WiFi module is used for communication between the main control unit and the mobile terminal.

[0007] Further, the running wheel includes a wheel frame and a runner rotatably arranged on the wheel frame; the wheel frame is fixedly installed on the bottom plate of the experimental chamber.

[0008] Further, the motion data acquisition device is a Hall sensor, and the Hall sensor is installed at the top of the wheel frame; a magnet block is arranged at a preset position of the runner, and the magnet block is used to trigger the Hall sensor to collect the number of rotations and the real-time rotational speed of the runner.

[0009] Further, a plurality of anti-slip grooves are arranged on the circumferential wall surface of the runner; the plurality of anti-slip grooves are parallel to the rotation axis of the runner, and a flat gap is arranged between adjacent two anti-slip grooves.

[0010] Further, the real-time light stimulation device is a blue LED module.

[0011] Further, the reward delivery device is a water pump.

[0012] Further, a food tray is further included; the food tray is arranged on the bottom plate of the experimental chamber and is directly below the outlet of the reward delivery device.

[0013] The present invention also provides an experimental method for animal running wheel conditioned reflex, using the experimental device for animal running wheel conditioned reflex; Experimental method for animal wheel conditioning, including: Placing an animal in a running wheel; when the animal moves in the running wheel, using a motion data acquisition device to collect the running data of the running wheel; when the running data of the running wheel reaches a preset motion target, using a control terminal to control the lighting stimulation device to turn on and control the reward delivery device to turn on; After the animal is placed in the running wheel, start using a monitoring camera to collect the animal behavior video data in the experimental chamber until the end of the experiment.

[0014] Compared with the prior art, the beneficial effects of the present invention are: The present invention provides an experimental device for animal wheel conditioning, integrating a monitoring camera, a lighting stimulation device, a motion data acquisition device and a reward delivery device in the experimental chamber to simultaneously meet the experimental requirements of animal behavior observation, conditioning training, motion data acquisition and food reward implementation, greatly simplifying the experimental process of animal wheel conditioning and improving the experimental efficiency; among them, the running data of the running wheel is accurately collected by the motion data acquisition device to obtain accurate and detailed animal motion information, providing a reliable basis for in-depth research on the behavior pattern, motion ability of animals and the relationship with conditioning; secondly, according to the running data of the running wheel, the lighting stimulation device and the reward delivery device are controlled by the control terminal to realize the delivery of light stimulation and food reward to the animal according to the requirements of the experimental design, so as to establish a conditioned reflex between the animal and the lighting stimulation; through the automatic control of the control terminal, the human intervention is reduced, the generation of human error is reduced, and the scientificity and accuracy of the experiment are improved; the experimental device described in the present invention has multiple functions and automatic control capabilities, and the accurate data acquisition and automatic experimental condition adjustment contribute to improving the experimental quality, obtaining more accurate and valuable experimental results, making the experimental process more efficient and convenient, and providing a broader experimental space for animal behavior research. Description of the Drawings

[0015] Figure 1 Stereoscopic cross-sectional view of the experimental device for animal wheel conditioning provided for the embodiment; Figure 2 Right cross-sectional view of the experimental device for animal wheel conditioning provided for the embodiment; Figure 3 Front cross-sectional view of the experimental device for animal wheel conditioning provided for the embodiment; Figure 4 Top view of the experimental device for animal wheel conditioning provided for the embodiment.

[0016] Among them, 1 is the experimental cabin, 2 is the monitoring camera, 3 is the running wheel, 4 is the light stimulation device, 5 is the motion data acquisition device, 6 is the reward delivery device, 7 is the food tray, 8 is the control terminal; 101 is the wiring hole. Detailed implementation manner

[0017] In order to make the technical problems, technical solutions and beneficial effects solved by the present invention clearer and more understandable, the following specific embodiments are used to further elaborate on the present invention. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0018] Embodiment As shown in the attached Figures 1-4 As shown, this embodiment provides an experimental device for animal wheel conditioning, including an experimental cabin 1, a monitoring camera 2, a running wheel 3, a light stimulation device 4, a motion data acquisition device 5, a reward delivery device 6, a food tray 7, a control terminal 8, a host computer and a mobile terminal.

[0019] The experimental cabin 1 is a hollow cabin structure. The monitoring camera 2, the running wheel 3, the light stimulation device 4, the motion data acquisition device 5, the reward delivery device 6 and the food tray 7 are all arranged inside the experimental cabin 1, and the control terminal 8 is arranged outside the experimental cabin 1.

[0020] The monitoring camera 2 is arranged at the center of the top of the experimental cabin 1; the running wheel 3 is installed at a preset position on the bottom of the experimental cabin 1; the light stimulation device 4 is installed on the left inner wall of the experimental cabin 1; the motion data acquisition device 5 is installed on the running wheel 3; the reward delivery device 6 is installed on the right inner wall of the experimental cabin 1; the food tray 7 is arranged on the bottom of the experimental cabin 1 and is placed directly below the reward delivery device 6; the control terminal 8 is arranged outside the experimental cabin 1 and is fixed on the outside of the rear end plate of the experimental cabin 1; a communication connection is established between the control terminal 8 and the host computer and the mobile terminal.

[0021] The monitoring camera 2 is used to collect the animal behavior video data in the experimental cabin 1 and send the animal behavior video data in the experimental cabin 1 to the control terminal 8.

[0022] The running wheel 3 serves as a carrier for animal movement to realize the running wheel movement of animals; the running wheel 3 includes a wheel frame and a runner. The wheel frame is fixedly installed on the bottom plate of the experimental cabin 1, and the runner is rotatably installed on the wheel frame; that is, the wheel frame serves as a support mechanism for the runner, enabling the runner to rotate around its axis.

[0023] The light stimulation device 4 is used to emit flickering light according to a preset flickering duration and flickering intensity to train the conditioned reflex of animals; wherein, the light stimulation device 4 is a blue LED module.

[0024] The motion data acquisition device 5 is used to acquire the operation data of the running wheel 3; wherein, the operation data of the running wheel 3 includes the number of rotation circles and the real-time rotation speed of the runner in the running wheel 3; specifically, the motion data acquisition device 5 adopts a Hall sensor, and the Hall sensor is installed at the top of the wheel frame; a magnet block is arranged at a preset position of the runner, and the magnet block is used to trigger the Hall sensor to acquire the number of rotation circles and the real-time rotation speed of the runner; wherein, by installing the Hall sensor at the top of the wheel frame and sensing the magnetic strength of the magnet block through the Hall sensor, the accurate monitoring of the number of rotation circles and the real-time rotation speed of the runner is realized, and further the accurate monitoring of the running wheel movement of the animal is realized.

[0025] It should be noted that a plurality of anti-slip lines are arranged on the circumferential wall surface of the runner, and the plurality of anti-slip lines are parallel to the rotation axis of the runner; wherein, a flat gap is arranged between adjacent two anti-slip lines; by arranging the anti-slip lines on the circumferential wall surface of the runner, it is ensured that the animal does not slip when moving in the runner, and the accuracy of the running wheel experiment is ensured.

[0026] The reward delivery device 6 is used to deliver food rewards; wherein, the food reward is fluid condensed milk; specifically, the reward delivery device 6 is a water pump to realize the quantitative delivery of the fluid condensed milk by using the water pump.

[0027] The feeding tray 7 is an open tray-like structure, and the feeding tray 7 is fixed directly below the outlet of the reward delivery device 6; wherein, a preset amount of fluid condensed milk is delivered into the feeding tray 7 through the reward delivery device 6 to reward the animal for eating.

[0028] The control terminal 8 is connected to the monitoring camera 2, the light stimulation device 4, the motion data acquisition device 5, the reward delivery device 6, the upper computer and the mobile terminal; wherein, a wiring hole 101 is arranged on the rear end plate of the experimental chamber 1, and the wiring hole 101 is a threading hole for the connection lines between the control terminal 8 and the monitoring camera 2, the light stimulation device 4, the motion data acquisition device 5 and the reward delivery device 6.

[0029] The control terminal 8 is used to control the light stimulation device 4 and the reward delivery device 6 according to the operation data of the running wheel 3. The control terminal 8 is further used to send the animal behavior video data, the operation data of the running wheel 3, the operation data of the light stimulation device 4, and the operation data of the reward delivery device 6 in the experimental chamber 1 to the host computer and the mobile terminal. Among them, the operation data of the light stimulation device 4 includes the flashing duration and the flashing intensity of the light stimulation device 4. The operation data of the reward delivery device 6 includes the flow rate and the start duration of the reward delivery device 6.

[0030] The control terminal 8 includes a main control unit, a voltage control module, a serial communication module, a WiFi module, a display module, and a button. The main control unit is connected to the voltage control module, the serial communication module, the WiFi module, and the display module. The main control unit is also connected to the monitoring camera 2, the light stimulation device 4, and the motion data acquisition device 5. Preferably, the main control unit uses an STM32 single-chip microcomputer, and the model of the STM32 single-chip microcomputer is STM32F103C8T6. The voltage control module is an N-MOSFET type voltage control module. The serial communication module is a CH-340 type USB to serial port module. The WiFi module is an ESP8266 type WiFi module. The display module is an OLED display screen.

[0031] The C13 port of the STM32 single-chip microcomputer is connected to the IO interface of the N-MOSFET type voltage control module. The ground wire of the N-MOSFET type voltage control module is connected to the ground wire of the STM32 single-chip microcomputer. The N-MOSFET module is used to control the power supply of the water pump, with one end connected to the 5V power supply and the positive electrode of the water pump, and the other end connected to the ground and the negative electrode of the water pump.

[0032] The A1 port of the STM32 single-chip microcomputer is connected to the S pole of the blue LED module. The positive electrode of the blue LED module is connected to the 3.3V power supply. The G pole of the blue LED module is connected to the ground. The B14 port of the STM32 single-chip microcomputer is connected to the OUT pole of the Hall sensor. The VCC of the Hall sensor is connected to the 3.3V power supply. The GND of the Hall sensor is grounded.

[0033] The A9 port of the STM32 single-chip microcomputer is connected to the RXD pole of the CH-340 type USB to serial port module, the A10 port of the STM32 single-chip microcomputer is connected to the TXD pole of the CH-340 type USB to serial port module, the GND of the CH-340 type USB to serial port module is grounded, and the VCC of the CH-340 type USB to serial port module is connected to the 3.3V power supply; the B8 port of the STM32 single-chip microcomputer is connected to the SCL pole of the OLED display screen, the B9 port of the STM32 single-chip microcomputer is connected to the SDA pole of the OLED display screen, the VCC of the OLED display screen is connected to the 3.3V power supply, and the GND of the OLED display screen is grounded.

[0034] The GND of the ESP8266 type WiFi module is connected to the GND of the CH-340 type USB to serial port module, the RXD of the ESP8266 type WiFi module is connected to the TXD of the CH-340 type USB to serial port module, the VCC of the ESP8266 type WiFi module is connected to the 3.3V power supply of the CH-340 type USB to serial port module, and the TXD of the ESP8266 type WiFi module is connected to the CH-340 type USB to serial port module; the first button is connected to the B1 port and the ground of the STM32 single-chip microcomputer, and the second button 2 is connected to the B11 port and the ground of the single-chip microcomputer.

[0035] The main control unit is used to output a light stimulation control signal and a reward delivery control signal according to the running data of the running wheel 3 and in combination with a preset conditioned reflex rule; wherein, the preset conditioned reflex rule includes the increasing relationship between the food reward amount and the number of rotations or the real-time rotation speed of the runner, the initial default value of the light stimulation duration, and the initial default value of the food reward amount; preferably, the increasing relationship between the food reward amount and the number of rotations or the real-time rotation speed of the runner is as follows:

[0036] Wherein, is the increment value of the food reward amount, is the number of rotations or the real-time rotation speed of the runner.

[0037] Preferably, the initial default value of the light stimulation duration is 300ms, and the initial default value of the food reward amount is 0.1mL.

[0038] Specifically, send a light stimulation control signal to the light stimulation device 4 to trigger the light stimulation device 4 to emit flashing light according to the preset flashing duration and flashing intensity; send a reward delivery control signal to the voltage control module to trigger the voltage control module to generate a start-stop instruction to the reward delivery device 6; wherein, the start-stop instruction is used to control the reward delivery device 6 to start or stop; it should be noted that the main control unit is also used to adjust the monitoring angle of the monitoring camera 2.

[0039] The serial communication module is used for communication between the main control unit and the host computer.

[0040] The WiFi module is used for communication between the main control unit and the mobile terminal.

[0041] The display module is used to display the running data of the running wheel 3 and the running data of the reward delivery device 6, so as to display the number of rotations and the real-time rotation speed of the rotating wheel, and the current reward intensity in real time; preferably, the display module adopts an OLED module, and the OLED module has a multi-level menu function.

[0042] The button is connected to the main control unit and is used to implement function selection or switching of the main control unit; wherein, the knob includes a pressing operation and a rotating operation; the pressing operation is used to implement function selection, and the rotating operation is used to implement function switching.

[0043] Optionally, this embodiment further includes an ST-LINK V2 programmer; the ST-LINK V2 programmer is used to burn a preset program into the main control unit; wherein, the preset program includes computer programs for implementing motion data acquisition, conditioned reflex control, and data transmission functions; specifically, the SWCLK port of the STM32 single-chip microcomputer is connected to the SWCLK port of the ST-LINK V2 programmer, and the SWDIO port of the STM32 single-chip microcomputer is connected to the SWDIO port of the ST-LINK V2 programmer; the 3.3V interface of the ST-LINK V2 programmer is connected to the 3.3V power supply, and the GND of the ST-LINK V2 programmer is grounded.

[0044] The host computer is used to receive the animal behavior video data in the experimental chamber 1, the running data of the running wheel 3, the running data of the light stimulation device 4, and the running data of the reward delivery device 6 through the serial communication module, and analyze the animal behavior video data in the experimental chamber 1, the running data of the running wheel 3, the running data of the light stimulation device 4, and the running data of the reward delivery device 6 to obtain the relationship between the activity frequency of the animal and the light stimulation, the running wheel movement, and the reward intensity.

[0045] Specifically, the activity frequency of the animal is obtained by analyzing the animal behavior video data in the experimental chamber 1 through the YOLO model preset in the host computer. Among them, the preset YOLO model is a pre-trained YOLOv5 model or YOLOv8 model. When analyzing the animal behavior video data in the experimental chamber 1, the running data of the running wheel 3, the running data of the light stimulation device 4, and the running data of the reward delivery device 6 to obtain the relationship between the animal's activity frequency and light stimulation, running wheel movement, and reward intensity, Matlab software is used to obtain a relationship curve characterizing the relationship between the animal's activity frequency and light stimulation, running wheel movement, and reward intensity through a preset fitting function or algorithm.

[0046] The mobile terminal is used to query the animal behavior video data in the experimental chamber 1, the running data of the running wheel 3, the running data of the light stimulation device 4, and the running data of the reward delivery device 6. The mobile terminal is also used to modify the preset parameters in the main control unit. Among them, the preset parameters of the main control unit include preset conditioned reflex rules, control parameters of the light stimulation device 4, and control parameters of the reward delivery device 6.

[0047] In this embodiment, the activity frequency of the animal is analyzed through the YOLO model preset in the host computer as follows: Obtain the animal behavior video data in the experimental chamber 1, and read each frame of the video image from the animal behavior video data in the experimental chamber 1. Then, identify the animal feature information from the read video image to obtain the position information and status information of the animal in the video image, and generate an ethogram heat map of the animal. Analyze the ethogram heat map of the animal to obtain the activity frequency of the animal. Among them, the activity frequency of the animal is characterized in the form of an anxiety and depression rating scale.

[0048] Working principle and experimental method: When the experimental device for animal running wheel conditioned reflex described in this embodiment works, it is as follows: Place the animal in the running wheel 3. When the animal moves in the running wheel 3, use the motion data acquisition device 5 to collect the running data of the running wheel 3. When the running data of the running wheel 3 reaches the preset motion target, use the control terminal 8 to control the light stimulation device 4 to turn on and control the reward delivery device 6 to turn on. After the animal is placed in the running wheel 3, start using the monitoring camera 2 to collect the animal behavior video data in the experimental chamber 1 until the experiment ends.

[0049] Taking the running wheel experiment of mice as an example, when using the experimental device for animal running wheel conditioned reflex described in this embodiment for the experiment, it is as follows: Place the mouse in the runner of the running wheel 3; when the mouse moves on the runner, use a Hall sensor to detect the magnet block attached to the runner to record the number of rotations and the real-time rotation speed of the runner; when the number of rotations or the real-time rotation speed of the runner reaches a preset value, the control terminal 8 controls the lighting stimulation device 4 to turn on and provide a signal for the mouse to eat; subsequently, control the reward delivery device 6 to turn on and extract a preset amount of condensed milk into the food tray; at the same time, use the monitoring camera 2 to detect whether the mouse shows an eating tendency, that is, whether the mouse continuously stays near the food, and record the magnitude of each eating tendency.

[0050] Data analysis: After the experiment, transmit the animal behavior video data in the experimental chamber 1, the operation data of the running wheel 3, the operation data of the lighting stimulation device 4, and the operation data of the reward delivery device 6 to the host computer, and use the host computer for data analysis to obtain the relationship between the animal's activity frequency and lighting stimulation, running wheel movement, and reward intensity; among them, when performing data analysis, the real-time rotation speed and the number of rotations of the runner are respectively represented as the vertical axis or the horizontal axis, and the relationship between the reward function, the reward quantity parameter, and the preset target is fitted through a preset fitting function or algorithm; the preset fitting function or algorithm is, for example, the Fourier fitting algorithm or the polynomial fitting algorithm; through the relationship between the fitted reward function, the reward quantity parameter, and the preset target, and combined with the ethogram heat map of the animal analyzed from the animal behavior video data in the experimental chamber 1, analyze and obtain the relationship between the animal's activity frequency and lighting stimulation, running wheel movement, and reward intensity.

[0051] The experimental device for animal runner conditioned reflex of the present invention can real-time monitor the running wheel movement data of animals and realize automatic reward delivery through the conditioned reflex mechanism, thereby providing an efficient and accurate experimental device for animal ethology research; by setting a monitoring camera, a running wheel, a lighting stimulation device, a motion data acquisition device, and a reward delivery device in the experimental chamber, and connecting the monitoring camera, the lighting stimulation device, the motion data acquisition device, and the reward delivery device to the control terminal; when the animal moves in the running wheel, the monitoring camera can be used to real-time monitor and record the position of the animal and the movement activity of each area; by setting a Hall sensor and a magnet block on the running wheel to monitor the number of rotations and the real-time rotation speed of the runner in the running wheel, realize the accurate identification of the animal's movement information; use the lighting stimulation device to train the animal's conditioned reflex; use the reward delivery device to quantitatively feed fluid food; the external control terminal is responsible for recording and adjusting the data and operations of the monitoring camera, the lighting stimulation device, the motion data acquisition device, and the reward delivery device; connect the control terminal to the host computer to realize data transmission with the host computer, and then complete the preset data analysis task through the host computer; and connect the control terminal to the mobile terminal to provide users with modification and query operations.

[0052] The above embodiments are merely one of the implementation manners capable of implementing the technical solution of the present invention. The scope of protection required by the present invention is not limited only by this embodiment, but also includes any changes, substitutions and other implementation manners that are easily conceivable by any person skilled in the art within the technical scope disclosed by the present invention.

Claims

1. An experimental device for animal wheel conditioning, characterized in that, It includes an experimental chamber (1), as well as a monitoring camera (2), a running wheel (3), a light stimulation device (4), a motion data acquisition device (5), and a reward delivery device (6) arranged inside the experimental chamber (1); The monitoring camera (2) is used to collect the animal behavior video data inside the experimental chamber (1); The light stimulation device (4) is used to emit flashing light to train the conditioned reflex of animals; The motion data acquisition device (5) is used to collect the operation data of the running wheel (3); The reward delivery device (6) is used to deliver food rewards; It further includes a control terminal (8); the control terminal (8) is connected to the monitoring camera (2), the light stimulation device (4), the motion data acquisition device (5), and the reward delivery device (6); wherein, the control terminal (8) is used to control the light stimulation device (4) and the reward delivery device (6) according to the operation data of the running wheel (3).

2. The experimental device for animal wheel conditioning according to claim 1, wherein It further includes a host computer and a mobile terminal connected to the control terminal (8); The control terminal (8) is used to send the animal behavior video data, the operation data of the running wheel (3), the operation data of the light stimulation device (4), and the operation data of the reward delivery device (6) inside the experimental chamber (1) to the host computer and the mobile terminal; The host computer is used to analyze the animal behavior video data, the operation data of the running wheel (3), the operation data of the light stimulation device (4), and the operation data of the reward delivery device (6) inside the experimental chamber (1) to obtain the relationship between the activity frequency of animals and light stimulation, running wheel movement, and reward intensity; The mobile terminal is used to query the animal behavior video data, the operation data of the running wheel (3), the operation data of the light stimulation device (4), and the operation data of the reward delivery device (6) inside the experimental chamber (1).

3. The experimental device for animal wheel conditioning according to claim 2, characterized in that, The control terminal (8) includes a main control unit, as well as a voltage control module, a serial communication module, and a WiFi module all connected to the main control unit; The main control unit is used to output a light stimulation control signal and a reward delivery control signal according to the operation data of the running wheel (3) and based on a preset conditioned reflex rule; wherein, the light stimulation control signal is used to trigger the light stimulation device (4) to emit flashing light; the reward delivery control signal is used to trigger the voltage control module to generate a start / stop instruction to the reward delivery device (6); The serial communication module is used for communication between the main control unit and the host computer; The WiFi module is used for communication between the main control unit and the mobile terminal.

4. The experimental device for animal wheel conditioning according to claim 1, characterized in that, The running wheel (3) includes a wheel frame and a runner rotatably arranged on the wheel frame; the wheel frame is fixedly installed on the bottom plate of the experimental chamber (1).

5. An experimental device for animal wheel conditioning according to claim 4, characterized in that, The motion data acquisition device (5) is a Hall sensor, and the Hall sensor is installed at the top of the wheel frame; a magnet block is arranged at a preset position of the runner, and the magnet block is used to trigger the Hall sensor to collect the number of rotations and the real-time rotation speed of the runner.

6. An experimental device for animal wheel conditioning according to claim 4, characterized in that A plurality of anti-slip grooves are provided on the circumferential wall surface of the runner; the plurality of anti-slip grooves are parallel to the rotation axis of the runner, and a flat gap is provided between two adjacent anti-slip grooves.

7. An experimental device for animal wheel conditioning according to claim 1, characterized in that, The real-time light stimulation device (4) is a blue LED module.

8. An experimental device for animal wheel conditioning according to claim 1, characterized in that, The reward delivery device (6) is a water pump.

9. An experimental device for animal wheel conditioning according to claim 8, characterized in that, It further includes a feeding tray (7); the feeding tray (7) is arranged on the bottom plate of the experimental chamber (1) and is directly below the outlet of the reward delivery device (6).

10. An experimental method for animal wheel-running conditioned reflex, characterized in that, Using the experimental device for animal runner conditioned reflex according to any one of claims 1-9; An experimental method for animal runner conditioned reflex, comprising: Placing an animal in the running wheel (3); when the animal moves in the running wheel (3), using the motion data acquisition device (5) to collect the running data of the running wheel (3); when the running data of the running wheel (3) reaches a preset motion target, using the control terminal (8) to control the light stimulation device (4) to turn on and control the reward delivery device (6) to turn on; After the animal is placed in the running wheel (3), start using the monitoring camera (2) to collect the animal behavior video data in the experimental chamber (1) until the experiment ends.

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