Preparation method and application of enzymolysis ginseng and polygonum multiflorum kidney-tonifying composition

Abstract

The invention relates to a preparation method and application of an enzymolysis ginseng and polygonum multiflorum kidney-tonifying composition, and the enzymolysis ginseng and polygonum multiflorum kidney-tonifying composition is prepared from the following raw materials: ginseng and polygonum multiflorum (synthesized by a glucosidase targeted enzymolysis technology), oyster peptide, icariin, rhizoma polygonati powder, sorbitol, microcrystalline cellulose, zinc gluconate, silicon dioxide and magnesium stearate. The enzyme for synthesizing ginsenoside is mainly glucosidase. Starting from an enzymolysis technology, the effective components of the ginseng and the polygonum multiflorum are increased, and the composition which is safe, non-toxic and remarkable in effect is provided for male crowds with weak kidney functions and insufficient energy and physical energy by combining the formula, reasonable proportion and reasonable selection of raw materials of the product, and has a wide market prospect.

Description

Technical Field

[0001] The present invention relates to the fields of enzymatic hydrolysis technology and functional foods. More specifically, it relates to a preparation method and application of an enzymatically hydrolyzed ginseng and fleece-flower root kidney-tonifying composition. Background Art

[0002] Functional foods, also known as health-care foods, refer to foods with specific nutritional and health-care functions. They are suitable for specific populations to consume, aiming to regulate the body's functions but not for treatment purposes. Their scope is extensive, including enhancing the human body's constitution, preventing diseases, restoring health, regulating the body's rhythm, and delaying aging, etc. With the popularization and development of the concept of functional foods, it has become an important branch of the global food industry. Especially in the face of social problems such as population aging and the increase in chronic diseases, its demand and importance are even more prominent.

[0003] In the prior art, for example, the function of ginseng and fleece-flower root disclosed in Patent CN 115969753 A is a hair-care composition containing extracts of ginseng and fleece-flower root and its products. This composition has an effect on hair loss, but it is single-targeted and fails to have an effect on improving the human body's kidney function, energy, and physical strength. Of course, the enzymatic hydrolysis technology is not introduced. Generally, biological enzymatic hydrolysis can increase the content of active ingredients in traditional Chinese medicines. It is found that the present invention uses the enzymatic hydrolysis technology for the first time.

[0004] Therefore, from the perspective of meeting human needs, it is very necessary to use the enzymatic hydrolysis technology and safe and practical products under the enzymatic hydrolysis technology. Summary of the Invention

[0005] The purpose of the present invention is to provide a preparation method and application of an enzymatically hydrolyzed ginseng and fleece-flower root kidney-tonifying composition. This product uses the composition under the enzymatic hydrolysis technology to provide a safe, non-toxic, and highly effective composition for people with weak male kidney function, insufficient energy, and physical strength.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] A preparation method and application of an enzymatically hydrolyzed ginseng and fleece-flower root kidney-tonifying composition: 65% ginseng and fleece-flower root (targeted enzymatic hydrolysis with glucosidase), 4% oyster peptide, 4% icariin, 3% polygonatum sibiricum powder, 10% sorbitol, 4% microcrystalline cellulose, 5% zinc gluconate, 2.5% silicon dioxide, 2.5% magnesium stearate, which can tonify male kidney deficiency, significantly improve the conditions of insufficient energy, physical decline, and soreness and weakness of the waist and knees, and has no side effects under reasonable dosage.

[0008] Optionally, the targeted enzyme: According to the conventional fermentation process of Escherichia coli, induced by IPTG, collect the bacterial cells, wash the bacterial cells, and centrifuge after disruption to obtain the supernatant, which is the crude enzyme solution.

[0009] Another object of the present invention is to provide an enzymatically hydrolyzed ginseng and fleece-flower root kidney-tonifying composition and its preparation method. Optionally, it includes the following steps:

[0010] S1: Ginseng and fleece-flower root (enzymatic hydrolysis targeted by glucosidase), selection of enzymes in the enzyme library: The glucosidase is the bgpA enzyme derived from Terrabacter ginsenosidimutans.

[0011] S2: Verification method for the selected effect of the enzymatically hydrolyzed ginseng and fleece-flower root extract:

[0012] The glucosidase enzyme solution is mixed with the ginseng and fleece-flower root extract in a ratio of 1:10, and reacted at 45°C for 5 h. After the reaction, 1 / 10 volume of water-saturated n-butanol is added, mixed evenly and centrifuged. The n-butanol layer is quantitatively analyzed by HPLC.

[0013] S3: Chromatographic conditions: Lunna NH2 chromatographic column (4.6 mm×150 mm, 5 μm); mobile phase: gradient elution of acetonitrile-water; flow rate: 1.0 mL·min-1; detection wavelength: 203 nm; column temperature: 30°C; injection volume: 20 μL. The gradient elution conditions are as follows: mobile phase: acetonitrile (A): water (B), gradient elution (0 - 20 min, A: 25→40; 20 - 33 min, A: 40→60; 33 - 37 min, A: 46; 37 - 45 min, A: 46→50; 45 - 62 min, A: 50→70; 62 - 65 min, A: 70→90).

[0014] S4: Replace and select according to the treatment method of the example: Replace the enzyme with PBS, and the other processes are the same.

[0015] S5: Enzymatically hydrolyze the ginseng and fleece-flower root extract: The glucosidase enzyme solution is mixed with the ginseng and fleece-flower root extract in a ratio of 1:10, and reacted at 45°C for 5 h. After the reaction, 1 / 10 volume of water-saturated n-butanol is added, mixed evenly and centrifuged. The n-butanol layer is quantitatively analyzed by HPLC.

[0016] S6: Results: The content of rare ginsenoside Rh2 in the enzymatically hydrolyzed ginseng and fleece-flower root extract is shown in Table 1. It can be seen that during the process of enzymatically hydrolyzing the ginseng and fleece-flower root extract with glucosidase, ginsenoside Rg3 is effectively converted into rare ginsenoside Rh2, which is more beneficial to improving the bioavailability.

[0017] Table 1 Content of rare ginsenoside Rh2 in the enzymatically hydrolyzed ginseng and fleece-flower root extract (mass percentage content)

[0018] S7: Large-scale preparation of enzymatic ginseng and Polygonum multiflorum extract: Expand production based on the above. After the enzymatic hydrolysis is completed, the volume is reduced to 1 times, and then freeze-dried overnight using a vacuum freeze dryer to obtain the enzymatic ginseng and Polygonum multiflorum extract. For subsequent production, refer to Figure 1 Process flow chart. The quality standards of raw and auxiliary materials refer to Figure 2 Selection of raw and auxiliary materials. The key control points of the entire process are mixing, tableting, and inner packaging.

[0019] Instruction Manual

[0020] Figure 1 Production process flow chart

[0021] Figure 2 Selection of raw and auxiliary materials DETAILED DESCRIPTION

[0022] The present invention is further described below in conjunction with embodiments, but the scope of protection claimed by the present invention is not limited to the scope described in the embodiments.

[0023] Example 1

[0024] A preparation method and application of an enzymatically hydrolyzed ginseng and Polygonum multiflorum kidney-tonifying composite material, comprising 65% ginseng and Polygonum multiflorum (targeted enzymatic hydrolysis by glucosidase), 4% oyster peptide, 4% icariin, 3% polygonatum powder, 10% sorbitol, 4% microcrystalline cellulose, 5% zinc gluconate, 2.5% silicon dioxide, and 2.5% magnesium stearate.

[0025] The enzymatically hydrolyzed ginseng and polygonum multiflorum kidney-tonifying composite material can be prepared by the above method.

[0026] Effect evaluation: 300 volunteers aged 30, 35, 40, 50, 55 and 60 were selected, with weak renal function, physical exhaustion, energy decline, weak waist and knees, etc. The effect of the composition of the present invention was evaluated for the volunteers aged 30-60 years old.

[0027] How to use: A course of treatment is 3 days. People aged 30-60 will feel the effect within 3 days, with a sense of blood rushing in the body and no discomfort. People aged 30-60 will feel the effect within 3 days, with significant improvement in energy decline and weakness in waist and knees.

[0028] Evaluation results

[0029] Table 2 Evaluation of the efficacy of the enzymatically hydrolyzed ginseng and Polygonum multiflorum kidney-tonifying composition

[0030] As can be seen from Table 2 above, the enzymatically hydrolyzed ginseng and Polygonum multiflorum kidney-tonifying composition of the present invention has the advantages of better supplementing the weak kidney function, improving physical strength, enhancing energy and relieving the phenomenon of soreness of waist and knees.

[0031] The above are only the preferred embodiments of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions falling within the concept of the present invention belong to the protection scope of the present invention. It should be noted that for those of ordinary skill in the art, several improvements and refinements made without departing from the principle of the present invention should also be regarded as within the protection scope of the present invention.

Claims

1. A preparation method and application of an enzymatically hydrolyzed ginseng and polygonum multiflorum composition for tonifying the kidney, characterized in that, It includes the following raw materials by mass fraction: glucosidase-targeted enzymatically hydrolyzed ginseng and polygonum multiflorum extract 65%, oyster peptide 4%, icariin monoside 4%, polygonatum sibiricum powder 3%, sorbitol 10%, microcrystalline cellulose 4%, zinc gluconate 5%, silicon dioxide 2.5%, magnesium stearate 2.5%.

2. The ginseng and polygonum multiflorum thunb composition for invigorating the kidney according to claim 1 and its preparation method, characterized in that, The content and purity of Rh2 in ginseng and polygonum multiflorum are further increased through the targeted technology of enzymatic hydrolysis.

3. A preparation method and application of an enzyme-hydrolyzed ginseng and fleeceflower root kidney-tonifying composition as described in claim 1 or 2, characterized in that, It includes the following steps: S1: Glucosidase-targeted enzymatic hydrolysis of ginseng and polygonum multiflorum. Selection of enzymes in the enzyme library: glycosidase; S2: Verification method after the selection of the action of the enzymatically hydrolyzed ginseng and polygonum multiflorum extract: Mix the glycosidase enzyme solution and the ginseng and polygonum multiflorum extract in a ratio of 1:10, and detect using a high-performance liquid system. S3: Wash ginseng and polygonum multiflorum, cut into pieces, and dry in the sun. Crush, add 3 times water and 7 times ethanol, reflux at 80 °C for 4 hours. Then concentrate by vacuum distillation to 1 times the volume. This is the ginseng and polygonum multiflorum extract. S4: Replace and select according to the treatment method of the example: Replace the enzyme with PBS, and the other processes are the same. S5: Result: The content of rare ginsenoside Rh2 in the enzymatically hydrolyzed ginseng and polygonum multiflorum extract is measured as shown in Table 3. It can be seen that during the process of enzymatically hydrolyzing the ginseng and polygonum multiflorum extract with glycosidase, ginsenoside Rg3 is effectively converted into rare ginsenoside Rh2, which is more conducive to improving bioavailability.

4. Preparation application of a kidney-tonifying composition containing an enzymatically hydrolyzed ginseng and polygonum multiflorum as described in claim 1 or 2.

5. The product according to claim 4, wherein the product is ginseng and polygonatum sibiricum tablet, characterized in that By mass percentage, it consists of the following components: glucosidase-targeted enzymatically hydrolyzed ginseng and polygonum multiflorum 65%, oyster peptide 4%, icariin monoside 4%, polygonatum sibiricum powder 3%, sorbitol 10%, microcrystalline cellulose 4%, zinc gluconate 5%, silicon dioxide 2.5%, magnesium stearate 2.5%. It supplements male kidney deficiency, significantly improves the conditions of lack of energy, physical decline, and soreness and weakness in the waist and knees, and has no side effects under reasonable dosage.

Images