Composition for promoting cartilage repair and maintaining joint health as well as preparation process and application of composition

Abstract

The invention discloses a composition for promoting cartilage repair and maintaining joint health as well as a preparation process and application thereof, and relates to the technical field of functional foods. The composition is prepared from the following components in parts by weight: 21.1 to 90 parts of a multi-protein complex, 0.1 to 3 parts of cherry powder, 0.01 to 2 parts of N-acetylglucosamine, 0.01 to 2 parts of radix puerariae powder, 0.1 to 1.5 parts of a calcium compound and 0.01 to 0.2 part of rhizoma curcumae longae, the multi-protein compound is prepared from 0.1 to 10 parts of ray collagen peptide, 1 to 30 parts of bone collagen peptide powder and 20 to 50 parts of fish collagen peptide. The composition provided by the invention can promote cartilage repair, achieves the purpose of daily maintenance of joint health, and has a good application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and particularly relates to a composition for promoting cartilage repair and maintaining joint health, its preparation process and applications. Background Art

[0002] Articular cartilage refers to the thin layer of hyaline cartilage covering the articular surface of bone ends. It is mainly composed of type II collagen, proteoglycans, chondrocytes and water. It has a certain elasticity and a smooth surface, and plays an important role in buffering mechanical stress, resisting pressure, enhancing bone elasticity and supporting joint activities. There is a lack of lymph and blood supply inside articular cartilage, and the nutrients and wastes of cartilage metabolism are absorbed and excreted through the diffusion of adjacent surrounding tissues. Therefore, the repair of damaged articular cartilage is slow.

[0003] Articular cartilage can be divided into four-layer structures according to morphology, from the surface to the inside are: the tangential zone, the transitional zone, the radial zone and the subchondral bone plate, and the radial zone and the subchondral bone plate are separated by the tidemark. Therefore, articular cartilage can be divided into four zones: the superficial zone, the middle zone, the deep zone and the highly mineralized calcified cartilage zone. The tangential zone is located in the outermost layer of cartilage and is mainly composed of collagen fibers and chondrocytes. The arrangement of collagen fibers in the transitional zone and the radial zone gradually turns oblique or perpendicular to the articular surface, and is mainly responsible for the resistance to pressure. The subchondral bone plays a mechanical support role for articular cartilage and is extremely important for maintaining joint homeostasis.

[0004] The clinical manifestations of cartilage injury include: (1) Joint pain: When cartilage is damaged, patients usually experience joint pain, which may be paroxysmal or activity-related; (2) Joint swelling: The injury causes an inflammatory reaction in the tissues around the joint, resulting in joint swelling; (3) A feeling of joint instability: The damage to cartilage may lead to irregularities on the joint surface, making the joint feel unstable during movement; (4) Limited joint movement: Due to the irregularities on the joint surface caused by cartilage injury, the normal range of motion may be restricted; (5) A feeling of joint friction: The irregular surface of cartilage may cause a feeling of friction during joint movement, especially when bending or straightening the joint.

[0005] Currently common methods for treating cartilage injury include: (1) Arthroscopic surgery: Through arthroscopic surgery, the damaged cartilage tissue is removed and cartilage transplantation is performed, transplanting healthy cartilage tissue to the damaged area; (2) Conservative treatment, including using non-steroidal anti-inflammatory drugs to relieve pain and control inflammation; (3) Minimally invasive surgery: Minimally invasive surgery can repair cartilage injury without opening the joint; (4) Joint replacement surgery: In the case of severe cartilage injury, joint replacement surgery is performed to improve and restore joint function. However, most treatment methods cannot produce enough new tissue to repair the damaged cartilage.

[0006] Chinese patent CN117045778A discloses a composition for improving the health of articular cartilage and its production process, wherein the composition comprises the following components by weight percentage: collagen peptide 5-10%, rosehip seed extract 10-20%, concentrated olive juice freeze-dried powder 1-5%, VC 0.1-0.3%, fruit juice powder 1-3%, and the balance is calcium supplement. The invention uses collagen peptide, rosehip seed extract, and concentrated olive juice freeze-dried powder as main ingredients, supplemented with VC, fruit juice powder, calcium supplement and other raw materials for bagging, which has the effect of improving the health of articular cartilage, can prevent and repair bone articular cartilage damage, and can increase bone strength, and has the function of regenerating and repairing articular cartilage.

[0007] At present, there are few studies on promoting cartilage repair, and the existing compositions for improving the health of articular cartilage are not effective and cannot meet the actual needs of patients. Therefore, it is urgent to develop a method for promoting articular cartilage damage safely and efficiently with few side effects through ordinary food. Summary of the invention

[0008] In order to overcome the above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a composition for promoting cartilage repair and maintaining joint health, and its preparation process and application.

[0009] The present invention uses a multi-protein complex (ray fish collagen peptide, bone collagen peptide powder, fish collagen peptide), cherry powder, N -Acetyl glucosamine, kudzu root powder, calcium compounds, turmeric, through scientific and reasonable proportions, especially the addition of cherry powder and kudzu root powder, can promote multiple protein complexes (ray fish collagen peptide, bone collagen peptide powder, fish collagen peptide), N -Acetyl glucosamine absorption, thus promoting cartilage repair.

[0010] To achieve the above-mentioned object of the invention, the technical solution of the present invention is as follows: In one aspect, the present invention provides a composition for promoting cartilage repair and maintaining joint health, wherein the composition is made of the following components in parts by weight: 21.1-90 parts of multi-protein complex, 0.1-3 parts of cherry powder, 0.01-2 parts of N-acetylglucosamine, 0.01-2 parts of kudzu root powder, 0.1-1.5 parts of calcium compound, 0.01-0.2 parts of turmeric; The multi-protein complex comprises 0.1-10 parts of ray collagen peptide, 1-30 parts of bone collagen peptide powder and 20-50 parts of fish collagen peptide.

[0011] Specifically, the composition is made of the following components by weight: 0.1 - 6 parts of skate collagen peptide, 10 - 20 parts of bone collagen peptide powder, 30 - 40 parts of fish collagen peptide, 0.5 - 1.5 parts of cherry powder, 0.1 - 1.5 parts of N - acetylglucosamine, 0.5 - 1.5 parts of kudzu root powder, 0.6 - 1 part of calcium compound, and 0.08 - 0.15 part of turmeric.

[0012] Further, the composition is made of the following components by weight: 1 part of skate collagen peptide, 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide, 1 part of cherry powder, N 1 part of N - acetylglucosamine, 1.5 parts of kudzu root powder, 1.5 parts of calcium compound, and 0.1 part of turmeric.

[0013] Specifically, the calcium compound is selected from one or more of calcium citrate, calcium carbonate, and calcium gluconate.

[0014] Further, the calcium compound is a combination of calcium citrate and calcium gluconate.

[0015] Specifically, the mass ratio of calcium citrate to calcium gluconate is (1 - 3):1.

[0016] Further, the mass ratio of calcium citrate to calcium gluconate is 2:1.

[0017] Specifically, the cherry is selected from one or more of acerola cherry, sweet cherry, and sour cherry.

[0018] Further, the cherry is sour cherry.

[0019] Specifically, the preparation method of the cherry powder is as follows: (1) Add 3 - 5 times the amount of water to the cherry to extract juice, obtaining the original cherry juice slurry; (2) Add enzymes to the original cherry juice slurry for enzymatic hydrolysis, inactivate the enzymes, concentrate, and dry to obtain the product.

[0020] Further, after juicing in step (1), it is sieved through a 40 - mesh sieve.

[0021] Specifically, the enzyme used for enzymatic hydrolysis in step (2) is selected from one or more of cellulase, pectinase, and hemicellulase.

[0022] Further, the enzyme used for enzymatic hydrolysis in step (2) is cellulase.

[0023] Specifically, the material - liquid ratio of the enzyme to the original cherry juice slurry in step (2) is (1 - 3):1.

[0024] Further, the material - liquid ratio of the enzyme to the original cherry juice slurry in step (2) is 1:1, 2.5:1, or 3:1.

[0025] Further, the ratio of the enzyme to the original cherry juice in step (2) is 2.5:1.

[0026] Specifically, the enzymolysis time in step (2) is 40 - 60 min; the enzymolysis temperature is 25 - 35 °C; Further, the enzymolysis time in step (2) is 50 min; the enzymolysis temperature is 25 °C.

[0027] Specifically, the temperature for inactivating the enzyme is 80 - 90 °C, and the time for inactivating the enzyme is 5 - 10 min.

[0028] Further, the temperature for inactivating the enzyme is 90 °C, and the time for inactivating the enzyme is 8 min.

[0029] Further, the concentration in step (2) includes but is not limited to vacuum concentration, freeze concentration, and salting - out concentration.

[0030] Still further, the concentration in step (2) is vacuum concentration.

[0031] Further, the degree of vacuum for the vacuum concentration in step (2) is 0.05 - 0.08 Mpa; Further, the temperature for the vacuum concentration in step (2) is 45 - 70 °C; Still further, the temperature for the vacuum concentration in step (2) is 60 °C.

[0032] Further, the concentration in step (2) is concentrated to a relative density of 1.25 - 1.30.

[0033] Specifically, the drying in step (2) includes but is not limited to vacuum drying.

[0034] Further, the conditions for the vacuum drying are 0.06 - 0.08 Mpa and the temperature is 70 - 80 °C.

[0035] Still further, the conditions for the vacuum drying are 0.08 Mpa and the temperature is 80 °C.

[0036] Further, the drying in step (2) is to dry until the water content of the extract is less than 3%.

[0037] Further, after drying in step (2), it is pulverized and sieved through a 60 - mesh sieve.

[0038] Specifically, the preparation method of the kudzu root powder is as follows: Add water to the kudzu root, boil it, make a pulp, filter, discard the precipitate, and dry the filtrate to obtain the kudzu root powder.

[0039] Further, the kudzu root is fresh kudzu root with the skin removed.

[0040] Furthermore, the boiling time is 60 - 100 min.

[0041] Furthermore, the amount of water added is 8 - 10 times the amount of kudzu root.

[0042] According to some embodiments of the present invention, the method for preparing kudzu root powder is as follows: After peeling fresh kudzu root, add 8 - 10 times the amount of water and boil for 60 - 100 min, beat into a pulp, filter, let the filtrate stand for 10 - 12 h, discard the precipitate, and dry the filtrate at 30 - 50 °C to obtain kudzu root powder.

[0043] On the other hand, the present invention provides a method for preparing the above - mentioned composition, comprising the following steps: (1) Crush and mix skate collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi - protein complex; (2) Mix cherry powder, kudzu root powder and turmeric to obtain mixture 1; (3) Add the multi - protein complex to mixture 2, and then add N - N - acetylglucosamine and calcium compound to obtain the composition.

[0044] On the other hand, the present invention provides the application of the above - mentioned composition in the preparation of products for promoting cartilage repair.

[0045] Specifically, the products include foods and drugs.

[0046] In the present invention, skate collagen peptide can stimulate the activity of osteoblasts, promote the proliferation of bone cells, improve the absorption and utilization efficiency of calcium, enable calcium compounds to be better deposited in bones, thereby enhancing bone density; bone collagen peptide powder can provide the raw materials required for the renewal and repair of cartilage; fish collagen peptide has a certain anti - inflammatory effect, can reduce joint inflammatory reactions, relieve joint discomfort by inhibiting inflammation, and improve the flexibility and stability of joints; cherry powder has the effect of promoting the absorption of multi - composite proteins and N - N - acetylglucosamine; N - N - acetylglucosamine is an important raw material for chondrocytes to synthesize proteoglycans and collagen fibers, can stimulate the proliferation and differentiation of chondrocytes, promote chondrocytes to produce proteoglycans with normal polymer structures, increase the repair ability of chondrocytes, help repair damaged or worn - out cartilage, and maintain the normal structure and function of cartilage; kudzu root powder has the effect of promoting the absorption of multi - composite proteins and N - N - acetylglucosamine; calcium compounds are an important component of bones and can provide raw materials for the renewal and repair of bones; curcumin in turmeric is a potent natural anti - inflammatory substance, can effectively reduce the damage to bones by inhibiting inflammatory reactions, and achieve the effect of maintaining the normal structure and function of bones.

[0047] The beneficial effects of the present invention are as follows: 1) For the first time, the present invention combines multiple composite proteins (skate collagen peptide, bone collagen peptide powder, fish collagen peptide), cherry powder, N N-acetylglucosamine, calcium compound, kudzu root powder, and turmeric in a scientific ratio to prepare a composition that can promote cartilage repair and maintain joint health.

[0048] 2) The present invention uses a special process to prepare cherry powder and kudzu root powder, which, when combined with the other components of the present invention, can better promote the absorption of multiple composite proteins and N N-acetylglucosamine, thereby achieving the purpose of promoting cartilage repair. Specific Embodiments

[0049] In order to make the technical means, creative features, achieved purposes, and functions of the present invention easy to understand, the following specific embodiments are used to further clarify the present invention. However, the following embodiments are only the preferred embodiments of the present invention, not all of them. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative efforts all fall within the protection scope of the present invention. In the following embodiments, unless otherwise specified, the operating methods used are all conventional operating methods, the equipment used is all conventional equipment, and the equipment materials used in each embodiment are the same.

[0050] The tart cherry used in the following embodiments is Montmorency tart cherry.

[0051] Example 1 Preparation of the Composition The composition includes, by weight: a multiple protein complex (1 part of skate collagen peptide (purchased from Shanghai Kaida Biotechnology Co., Ltd.), 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide), 1 part of cherry powder, N 1 part of N-acetylglucosamine, 1.5 parts of kudzu root powder, 1.5 parts of calcium compound (the mass ratio of calcium citrate to calcium gluconate is 2:1), and 0.1 part of turmeric.

[0052] The particle size of the multiple protein complex is as follows: 60 - 200 mesh accounts for 60%, 200 - 800 mesh accounts for 20%, and 800 - 2000 mesh accounts for 20%.

[0053] The preparation method of the composition is as follows: (1) Crush and mix skate collagen peptide, bone collagen peptide powder, and fish collagen peptide to obtain a multiple protein complex; (2) Mix cherry powder, kudzu root powder, and turmeric to obtain Mixture 1; (3) Add the multiple protein complex to Mixture 1, and then add N N-acetylglucosamine and calcium compound to obtain the composition.

[0054] The preparation of cherry powder includes the following steps: (1) Add 5 times the amount of water to cherries to extract juice, and pass through a 40-mesh sieve to obtain the original cherry juice slurry; (2) Add cellulase to the original cherry juice slurry for enzymatic hydrolysis. The material-liquid ratio of the enzyme to the original cherry juice slurry is 2.5:1. The enzymatic hydrolysis time is 50 min, the enzymatic hydrolysis temperature is 25 °C, inactivate the enzyme at 90 °C for 8 min, vacuum concentrate, the vacuum degree is 0.07 Mpa, the vacuum concentration temperature is 60 °C, concentrate to a relative density of 1.25 - 1.30, vacuum dry, the vacuum drying conditions are 0.08 Mpa and the temperature is 80 °C, dry until the moisture content of the extract is less than 3%, pulverize and pass through a 60-mesh sieve to obtain.

[0055] The preparation method of kudzu root powder is as follows: Peel fresh kudzu root and add 8 times the amount of water to boil for 80 min, use a wall breaker to make slurry, filter, let the filtrate stand for 12 h, discard the precipitate, dry the filtrate at 40 °C for 5 h, and pulverize to obtain kudzu root powder.

[0056] Example 2 Preparation of the composition The composition described above includes, by weight: multiple protein complexes (0.1 part of skate collagen peptide, 10 parts of bone collagen peptide powder, 30 parts of fish collagen peptide), 0.5 part of cherry powder, N 0.1 part of N-acetylglucosamine, 0.5 part of kudzu root powder, 0.6 part of calcium compound (the mass ratio of calcium citrate to calcium gluconate is 1:1), 0.08 part of turmeric.

[0057] The particle size of the multiple protein complex is as follows: the proportion of 60 - 200 mesh is 90%, the proportion of 200 - 800 mesh is 5%, and the proportion of 800 - 2000 mesh is 5%.

[0058] The preparation method of the composition is the same as that of Example 1.

[0059] The preparation of cherry powder includes the following steps: (1) Add 3 times the amount of water to cherries to extract juice, and pass through a 40-mesh sieve to obtain the original cherry juice slurry; (2) Add cellulase to the original cherry juice slurry for enzymatic hydrolysis. The material-liquid ratio of the enzyme to the original cherry juice slurry is 1:1. The enzymatic hydrolysis time is 50 min, the enzymatic hydrolysis temperature is 25 °C, inactivate the enzyme at 90 °C for 8 min, vacuum concentrate, the vacuum degree is 0.07 Mpa, the vacuum concentration temperature is 60 °C, concentrate to a relative density of 1.25 - 1.30, vacuum dry, the vacuum drying conditions are 0.08 Mpa and the temperature is 80 °C, dry until the moisture content of the extract is less than 3%, pulverize and pass through a 60-mesh sieve to obtain.

[0060] The preparation method of kudzu root powder is as follows: peel the fresh kudzu root, add 10 times the amount of water and boil for 100 minutes, beat, filter, let the filtrate stand for 12 hours, discard the precipitate, dry the filtrate at 40°C for 5 hours, and grind to obtain kudzu root powder.

[0061] Example 3 Preparation of composition The composition comprises, by weight: a multi-protein complex (6 parts of ray collagen peptide, 20 parts of bone collagen peptide powder, 40 parts of fish collagen peptide), 1.5 parts of cherry powder, N -1.5 parts of acetylglucosamine, 1.5 parts of kudzu root powder, 1 part of calcium compound (the mass ratio of calcium citrate to calcium gluconate is 3:1), and 0.15 parts of turmeric.

[0062] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0063] The preparation method of the composition is the same as that of Example 1.

[0064] The preparation of cherry powder comprises the following steps: (1) Add 5 times the amount of water to the cherries to squeeze the juice, and pass it through a 40-mesh sieve to obtain cherry juice; (2) Add cellulase to the cherry juice puree for enzymolysis, the solid-liquid ratio of enzyme to cherry juice puree is 3:1, the enzymolysis time is 60 minutes, the enzymolysis temperature is 35°C, the enzyme is inactivated at 90°C for 8 minutes, vacuum concentration is performed, the vacuum degree is 0.07Mpa, the vacuum concentration temperature is 60°C, the relative density is 1.25-1.30, vacuum drying is performed, the vacuum drying conditions are 0.08Mpa, the temperature is 80°C, and the water content of the extract is less than 3%, and the extract is crushed and passed through a 60-mesh sieve to obtain.

[0065] The preparation method of Pueraria powder is the same as that in Example 1.

[0066] Example 4 Preparation of composition The composition comprises, by weight: a multi-protein complex (10 parts of ray collagen peptide, 30 parts of bone collagen peptide powder, 50 parts of fish collagen peptide), 3 parts of cherry powder, N -2 parts of acetylglucosamine, 2 parts of kudzu root powder, 1.5 parts of calcium compound (the mass ratio of calcium citrate to calcium gluconate is 2:1), and 0.2 parts of turmeric.

[0067] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0068] The preparation of the composition is the same as in Example 1.

[0069] The preparation of cherry powder and kudzu root powder is the same as in Example 1.

[0070] Comparative Example 1 The only difference from Example 1 is that cherry powder is not added to the composition, and the rest is the same. The composition in Comparative Example 1 includes, by weight: Multi-protein complex (1 part of ray collagen peptide, 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide), N -1 part of acetylglucosamine, 1.5 parts of kudzu root powder, 1.5 parts of calcium compound (the mass ratio of calcium citrate to calcium gluconate is 2:1), and 0.1 part of turmeric.

[0071] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0072] The preparation method of Pueraria powder is the same as that in Example 1.

[0073] The preparation method of the composition is: (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex; (2) Mix kudzu root powder and turmeric to obtain mixture 1; (3) Add the multi-protein complex to mixture 1, and then add N -acetylglucosamine and a calcium compound to obtain a composition.

[0074] Comparative Example 2 The difference from Example 1 is that no kudzu root powder is added to the composition, and the rest are the same. The composition in Comparative Example 2 includes, by weight: a multi-protein complex (1 part of skate collagen peptide, 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide), 1 part of cherry powder, N -1 part of acetylglucosamine, 1.5 parts of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 2:1), and 0.1 part of turmeric.

[0075] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0076] The preparation method of cherry powder is the same as that of Example 1.

[0077] The preparation method of the composition is: (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex; (2) Mix cherry powder and turmeric to obtain mixture 1; (3) Add the multi-protein complex to mixture 1, and then add N -acetylglucosamine and a calcium compound to obtain a composition.

[0078] Comparative Example 3 The difference from Example 1 is that cherry powder and kudzu root powder are not added to the composition, and the rest are the same. The composition in Comparative Example 3 includes, by weight: a multi-protein complex (1 part of skate collagen peptide, 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide), N -1 part of acetylglucosamine, 1.5 parts of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 2:1), and 0.1 part of turmeric.

[0079] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0080] The preparation method of the composition is: (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex; (1) Add the multi-protein complex to turmeric and then add N -acetylglucosamine and a calcium compound to obtain a composition.

[0081] Comparative Example 4 The only difference from Example 1 is that the particle size proportions of the multiple protein complex are different. In Comparative Example 4, 60-200 mesh accounts for 40%, 200-800 mesh accounts for 40%, and 800-2000 mesh accounts for 20%.

[0082] Comparative Example 5 The only difference from Example 1 is that the skate collagen peptide is replaced with type II collagen peptide, and the rest is the same.

[0083] Experimental Example 1 Effect of the composition on cartilage repair 1 Reagents, consumables and instruments Reagents and consumables: transgenic cartilage green fluorescent zebrafish Tg (Co12ala: eGFP) (purchased from Huante Biotechnology), methylcellulose (sigma, CAS: 497-76-7), chondroitin sulfate A sodium salt (C758506-5g, Aladdin), dexamethasone (Source Leaf BR, 99%, S17003-5g).

[0084] Instruments: stereo microscope (MZ62, Mshot), microscope photography system (BX53, OLYMPUS, Japan).

[0085] 2 Experimental procedures Randomly select normal-developing Tg (col2a1a:EGFP) transgenic cartilage fluorescent zebrafish embryos at 2-3 dpf and place them in a six-well culture plate, 30 embryos per well. Without harming the embryos, remove the standard dilution water in the six-well plate, and quickly add 3 mL of the corresponding concentration of the composition to each well. Except for the blank control group, the remaining groups are added with 25 μM of dexamethasone to establish a zebrafish cartilage injury model. The positive control drug is 1 mg / mL of chondroitin sulfate. Cover the culture plate panel and wrap it with aluminum foil, and incubate it in the dark at (28.5 ± 1) °C in a biochemical incubator for 3 days. After 3 days, detect the green fluorescence intensity of the zebrafish.

[0086] 2.1 Experimental grouping Blank control group: containing 2-3 dpf zebrafish embryos and 3 mL of standard dilution water; Model control group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone prepared with standard dilution water; Positive control group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 1 mg / mL of chondroitin sulfate prepared with standard dilution water; Example 1 group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 0.5 mg / mL of the composition prepared in Example 1; Example 2 group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 0.5 mg / mL of the composition prepared in Example 2; Example 3 group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 0.5 mg / mL of the composition prepared in Example 3; Example 4 group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 0.5 mg / mL of the composition prepared in Example 4; Comparative example 1 group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 0.5 mg / mL of the composition prepared in Comparative example 1; Comparative example 2 group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 0.5 mg / mL of the composition prepared in Comparative example 2; Comparative example 3 group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 0.5 mg / mL of the composition prepared in Comparative example 3; Comparative example 4 group: containing 2-3 dpf zebrafish embryos and 25 μM of dexamethasone and 0.5 mg / mL of the composition prepared in Comparative example 4; Control group 5: Zebrafish embryos at 2-3 dpf, 25 μM of dexamethasone prepared with standard dilution water, and the composition prepared in Control group 5 at 0.5 mg / mL.

[0087] 2.2 Observation, photography, and data analysis Three days after treatment with the test substance, the zebrafish in each experimental group were placed under a fluorescence microscope for observation and photography. At least 10 zebrafish were randomly selected from each experimental group, fixed with 3% methylcellulose, and the green fluorescence of the craniofacial cartilage of the zebrafish was photographed and collected. The fluorescence intensity (A) of the craniofacial cartilage of the zebrafish was quantitatively statistically analyzed using Image J software. The repair ability of the composition on dexamethasone-induced cartilage damage was evaluated based on the statistical analysis results of this index. The statistical processing results were expressed as mean ± SE. Graphpad Prism software was used for drawing and statistical analysis. p <0.05 indicates that the difference is statistically significant.

[0088] Calculation formula for cartilage repair: Cartilage regeneration rate C = (A1 - A0) / A0 × 100%; In the formula, C - cartilage regeneration rate, %; A0 - fluorescence intensity of the craniofacial cartilage of zebrafish in the model control group (n = 10); A1 - fluorescence intensity of the craniofacial cartilage of zebrafish in each composition (n = 10).

[0089] 3 Experimental results The cartilage EGFP fluorescence intensity and cartilage regeneration rate of zebrafish in each group are shown in Table 1 below: Table 1 Cartilage EGFP fluorescence intensity and cartilage regeneration rate of zebrafish in each group

[0090] Note: Compared with the blank control group, *p < 0.05; compared with the model control group, #p < 0.05.

[0091] As can be seen from the above table, compared with the blank control group, the cartilage fluorescence intensity of the model control group was significantly reduced. Compared with the model control group, the cartilage regeneration rates of the compositions prepared in Examples 1-4 of the present application were 35.3%, 34.8%, 32.3%, and 34.2% respectively, indicating that the compositions of the present application have a good effect on promoting cartilage regeneration. One or two components were missing in Comparative Examples 1-3, and their effects on promoting cartilage regeneration were significantly reduced. In Comparative Example 4, the particle size ratio of the multi-protein complex, and in Comparative Example 5, replacing skate collagen peptide with type II collagen peptide made its effect on cartilage regeneration significantly lower than that of Examples 1-4 groups.

[0092] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A composition for promoting cartilage repair and maintaining joint health, characterized in that, The composition is made of the following components by weight: 21.1-90 parts of multi-protein complex, 0.1-3 parts of cherry powder, N - 0.01-2 parts of acetylglucosamine, 0.01-2 parts of kudzu root powder, 0.1-1.5 parts of calcium compound, 0.01-0.2 parts of turmeric; The described multi-protein complex comprises 0.1 - 10 parts of skate collagen peptide, 1 - 30 parts of bone collagen peptide powder, and 20 - 50 parts of fish collagen peptide.

2. The composition according to claim 1, wherein The composition is made of the following components by weight: 0.1-6 parts of ray collagen peptide, 10-20 parts of bone collagen peptide powder, 30-40 parts of fish collagen peptide, 0.5-1.5 parts of cherry powder, N - 0.1-1.5 parts of acetylglucosamine, 0.5-1.5 parts of kudzu root powder, 0.6-1 parts of calcium compound, and 0.08-0.15 parts of turmeric.

3. The composition according to any one of claims 1-2, characterized in that, The preparation method of the described cherry powder is as follows: (1) Add 3 - 5 times the amount of water to cherries to extract juice, obtaining the original cherry juice slurry; (2) Add an enzyme to the original cherry juice slurry for enzymatic hydrolysis, inactivate the enzyme, concentrate, and dry to obtain the cherry powder.

4. The composition according to claim 3, wherein The cherries described are selected from one or more of acerola cherries, tart cherries, and sweet cherries.

5. The composition according to claim 3, wherein The enzyme used for the enzymatic hydrolysis in step (2) is selected from one or more of cellulase, pectinase, and hemicellulase; the material-liquid ratio of the enzyme to the original cherry juice slurry is (1 - 3):1; the time for the enzymatic hydrolysis is 40 - 60 min; the temperature for the enzymatic hydrolysis is 25 - 35 °C.

6. The composition according to any one of claims 1-2, characterized in that, The preparation method of the described kudzu root powder is: Add water to kudzu root, boil it, make a pulp, filter, discard the precipitate, and dry the filtrate to obtain kudzu root powder.

7. The composition according to claim 6, wherein The time for the boiling is 60 - 100 min.

8. The composition according to any one of claims 1-2, characterized in that, The particle size of the described multi-protein complex is as follows: The proportion of 60 - 200 mesh is 50 - 90%, the proportion of 200 - 800 mesh is 5 - 30%, and the proportion of 800 - 2000 mesh is 1 - 20%.

9. A method for preparing the composition according to any one of claims 1-8, characterized in that, It includes the following steps: (1) Crush and mix the skate collagen peptide, bone collagen peptide powder, and fish collagen peptide to obtain the multi-protein complex; (2) Mix the cherry powder, kudzu root powder, and turmeric to obtain mixture 1; (3) Add Mixture 1 to the multi-protein complex, and then add N - N-acetylglucosamine and a calcium compound to obtain the composition.

10. Use of the composition according to any one of claims 1 - 8 in the preparation of a product for promoting cartilage repair.

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