Sterile plant flower water stock solution as well as preparation method and application thereof

Abstract

The invention relates to the technical field of skin care products, in particular to a sterile plant flower water stock solution and a preparation method and application thereof. The sterile plant flower water stock solution is prepared from the following raw materials in parts by mass: 5 to 30 parts of green rattan flower water, 5 to 30 parts of cancerous bulbophyllum flower water, 5 to 30 parts of strelitzia reginae flower water, 5 to 30 parts of maifangzi flower water, 0.05 to 1 part of pachymaran, 0.01 to 0.05 part of natto gum, 1 to 10 parts of snail mucus extract and 0.75 to 14 parts of elastin. Through a unique synergistic mechanism in the aspects of oxidation resistance, anti-inflammatory relieving and moisturizing and moistening when the green tuberous root flower water, the cancerous cancerous flower water, the strelitzia reginae flower water and the maifang flower water have a synergistic effect, skin inflammation is relieved synergistically from multiple brand-new angles, and red, swollen and pruritus symptoms caused by allergy and stimulation are relieved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and particularly to a sterile plant flower water stock solution, a preparation method thereof, and an application thereof. Background Art

[0002] With the deterioration of the environment and the change of lifestyle, skin sensitivity problems have become increasingly common. Environmental pollution, heavy metal infringement, over-cleaning, and the popularity of beauty acid peeling projects are all constantly damaging the skin barrier, resulting in a significant increase in the number of people with sensitive skin. As the body's first line of defense, the stratum corneum is crucial for maintaining skin moisture balance and isolating external stimuli. Moisturization is the basis for maintaining skin health, which can not only keep the skin moist, delay aging, but also prevent desquamation and other skin problems caused by dryness.

[0003] However, although there are a wide variety of moisturizing skin care products on the current market, products that can truly meet the strong moisturizing needs and are suitable for highly sensitive skin are extremely scarce. Summary of the Invention

[0004] The purpose of the present invention is to propose a sterile plant flower water stock solution and a preparation method thereof in view of the above-mentioned deficiencies of the prior art.

[0005] The first object of the present invention is to provide a sterile plant flower water stock solution, which comprises the following raw materials in parts by mass: 5-30 parts of jade vine flower water, 5-30 parts of cannonball flower water, 5-30 parts of strelitzia flower water, 5-30 parts of jasmine water, 0.05-1 part of pachymaran, 0.01-0.05 part of natto gum, 1-10 parts of snail mucus extract, and 0.75-14 parts of elastin.

[0006] Further, the preparation method of jade vine flower water is as follows: Wash and crush the freshly picked jade vine flowers, and then use the steam distillation method to collect the distillate to obtain crude flower water; Filter, centrifuge, and finely filter the crude flower water to obtain refined jade vine flower water.

[0007] Further, wash, disinfect, and air-dry the freshly picked and mature and intact cannonball tree flowers, then use supercritical carbon dioxide extraction to obtain cannonball flower extract, and then dilute the extract with sterile deionized water at a ratio of 1:10-20 and filter to obtain sterile cannonball flower water.

[0008] Further, the preparation method of strelitzia flower water is as follows: Wash and cut the freshly picked strelitzia flowers into sections, then extract with an ethanol aqueous solution of 40%-60% as the extraction solvent, then filter and evaporate the extract, and then dialyze and filter the concentrated solution to remove alcohol to obtain refined strelitzia flower water.

[0009] Furthermore, the preparation method of Jasminum officinale L. var. grandiflorum water is as follows: Wash the picked fresh Jasminum officinale L. var. grandiflorum and remove the surface moisture, then obtain 1-2 L of the initial distillate by water distillation method and continue distillation; Mix the initial distillate with the subsequent distillate, adsorb with activated carbon and then filter to obtain the refined Jasminum officinale L. var. grandiflorum water.

[0010] The second object of the present invention is to provide a preparation method of the sterile plant flower water stock solution as described above. Add jade vine flower water, cannonball flower water, strelitzia flower water, Jasminum officinale L. var. grandiflorum water, pachymaran, sodium polyacrylate, snail mucus extract and elastin into the emulsifying pot in accordance with certain mass parts in sequence, heat and stir and then perform homogenization treatment, then keep warm and stir. After all the materials are fully dissolved, perform moist heat sterilization to obtain the sterile plant flower water stock solution.

[0011] Furthermore, the temperature of heating and stirring is 80-85 °C, and the stirring speed is 150-200 r / min.

[0012] Furthermore, perform homogenization treatment for 2-5 min.

[0013] Furthermore, keep warm and stir for 15-30 min.

[0014] Furthermore, after all the materials are fully dissolved, sample and detect according to the "Technical Specifications for Cosmetics Safety". The qualified material is filtered through a 200-mesh filter cloth into the material container of the filling equipment, and then hot filling and capping are carried out; Finally, the filled product is subjected to high-temperature moist heat sterilization to obtain the sterile plant flower water stock solution.

[0015] The third object of the present invention is to provide an application of the sterile plant flower water stock solution as described above, which is used in moisturizing and anti-inflammatory skin care products for sensitive skin.

[0016] To sum up, the present invention has the following beneficial effects:

[0017] 1. Unique anti-inflammatory mechanism: When jade vine flower water, cannonball flower water, strelitzia flower water and Jasminum officinale L. var. grandiflorum water act synergistically in a certain proportion, they have a unique synergistic mechanism in terms of antioxidant, anti-inflammatory and soothing, and moisturizing and nourishing, and this mechanism is completely different from that of traditional chrysanthemum flower water; Jade vine flower water can regulate cell communication through its specific polysaccharide components and affect the transmission path of inflammatory signals; The active ingredients of cannonball flower water may act on specific enzyme systems in cells to inhibit the synthesis of inflammatory mediators; Strelitzia flower water can reduce the adsorption and invasion of inflammatory factors by changing the fluidity of cell membranes; Jasminum officinale L. var. grandiflorum water can precisely regulate the activity of immune cells and reduce inflammatory reactions from the source. The four kinds of flower waters cooperate with each other, synergistically reducing skin inflammation from multiple new angles, relieving the redness, swelling, itching symptoms caused by allergies and irritations, and the effect is better than using a single flower water, meeting the urgent needs of high-sensitivity skin people for high-efficiency and safe skin care products.

[0018] 2. Enhanced moisturizing synergy: The polysaccharides in the jade vine flower water form a unique three-dimensional moisturizing network, enhancing the skin's water-locking ability; the cannonball flower water promotes the expression of aquaporin proteins in cells, improving the cell hydration efficiency; the strelitzia flower water replenishes the moisture in the stratum corneum with its hydrophilic components; the jasmine flower water helps maintain the balance of the water-lipid film on the skin surface. The four work together to hydrate and lock water for the skin from the cellular level to the skin surface, significantly enhancing the skin's moisture level and moisturizing persistence.

[0019] 3. Natural and safe advantages: The key components selected in this invention, such as jade vine flower water, cannonball flower water, strelitzia flower water, jasmine flower water, poria cocos polysaccharide, natto gum, snail mucus extract, and elastin, all have clear and reliable natural sources.

[0020] Poria cocos polysaccharide plays multiple roles in skin care products mainly through strong moisturization (locking water, reducing TEWL), antioxidant (scavenging free radicals, promoting collagen synthesis), anti-inflammatory (inhibiting factors such as NF-KB), and immunomodulation (activating and repairing cells). It can improve dryness, delay aging, soothe sensitivity, etc., and is mild and safe, suitable for all skin types.

[0021] Natto gum (produced by fermenting natto bacteria) mainly plays roles of moisturization (forming a film to lock water), antioxidant (scavenging free radicals), anti-inflammatory (inhibiting pro-inflammatory factors), and promoting repair (enhancing the barrier) in skin care products. Its mucopolysaccharide structure can improve the product feel, and it is mild and low-irritating, suitable for soothing sensitive skin or anti-aging formulations.

[0022] Snail mucus extract is rich in natural polysaccharides, elastin, allantoin, and glycolic acid, and has functions of strong repair (promoting cell regeneration, accelerating wound healing), moisturizing and locking water (natural moisturizing factors + mucus film formation), antioxidant (scavenging free radicals), etc., and has a mild and skin-friendly texture.

[0023] Elastin has functions of efficient repair (promoting the proliferation of fibroblasts, accelerating skin regeneration), deep moisturization (locking water and enhancing the barrier function), anti-wrinkle and firming (supporting the elastic fiber structure), and soothing and anti-allergy (reducing inflammatory reactions). Its biocompatibility is excellent, suitable for stabilizing sensitive skin, and can significantly improve problems such as skin relaxation, fine lines, and dryness.

[0024] This not only ensures the safety and mildness of the product, minimizing the risk of irritation to sensitive skin, but also fully exploits the unique advantages of natural ingredients in skin care applications, providing pure and effective care for the skin. Detailed implementation manners

[0025] The following are specific examples of the present invention to further describe the technical solutions of the present invention, but the present invention is not limited to these examples.

[0026] The raw materials used in the examples are as follows:

[0027] Method for preparing flower water

[0028] 1. Preparation of jade vine flower water

[0029] (1) Collection: In the early morning during the full bloom period of jade vine, select healthy flowers without pests and diseases, carefully cut them with scissors, place them in a clean container, and process them as soon as possible.

[0030] (2) Cleaning and pretreatment: Gently wash the flowers in flowing pure water, remove impurities, and drain them on a gauze. Crush them moderately to improve the extraction rate.

[0031] (3) Extraction: Adopt the steam distillation method. Put the flowers into the distillation pot, add (5 - 10) times the mass of pure water, and seal the device. Heat to make the water boil, and the steam carries the volatile components and liquefies through the condenser tube. Collect the distillate to obtain the crude flower water.

[0032] (4) Filtration and refinement: Filter the crude flower water through multiple layers of filter paper, centrifuge at 3000 - 5000 r / min for 5 - 10 min, and then finely filter with a 0.22 μm microporous filter membrane to obtain the refined flower water, which is sealed and stored.

[0033] 2. Preparation of cannonball flower water

[0034] (1) Picking: Select mature and complete flowers, carefully pick them with sterile tools, put them in a sterile bag, and transfer them to the laboratory as soon as possible.

[0035] (2) Cleaning and disinfection: After rinsing with sterile water, soak them in a 0.1% - 0.3% sodium hypochlorite solution for 3 - 5 min for disinfection, rinse with a large amount of sterile water, and air-dry on filter paper.

[0036] (3) Extraction: Supercritical carbon dioxide extraction. Crush the flowers into powder, put them into the extraction kettle, with a pressure of 20 - 30 MPa, a temperature of 35 - 45 °C, a carbon dioxide flow rate of 20 - 30 L / h, and extract for 1 - 2 h. The extract is separated by reducing pressure and temperature in the separation kettle to obtain the cannonball flower extract.

[0037] (4) Preparation of flower water: Dilute the extract with sterile deionized water at a ratio of 1:(10 - 20), stir evenly, filter successively through 0.45 μm and 0.22 μm microporous filter membranes to obtain sterile cannonball flower water, which is sealed and stored refrigerated in a brown bottle.

[0038] 3. Preparation of strelitzia flower water

[0039] (1) Collection and selection: During the flowering period, select well-blooming flowers, cut them from the base of the flower stalk with a disinfected scissors, place them in a clean container, and prevent damage and contamination.

[0040] (2) Preliminary treatment: Rinse off dust and impurities with clean water, remove withered leaves and flower stalks with tweezers, absorb the moisture with a gauze, and cut them into 1 - 2 cm small sections.

[0041] (3) Extraction: An ethanol aqueous solution of 40%-60% is used as the extraction solvent, and the mass ratio of the flower segment to the solvent is 1:(8-12). Extraction is carried out at room temperature in the dark for 24-48h with regular stirring.

[0042] (4) Filtration and concentration: The extraction solution is filtered through filter paper to remove residues, and then concentrated under reduced pressure at 40-50°C using a rotary evaporator to 1 / 3-1 / 2 of the original volume.

[0043] (5) Alcohol removal and purification: The concentrated solution is dialyzed for 12-24h to remove alcohol, the water is changed 3-4 times, and then filtered through a 0.22μm microporous filter membrane to obtain purified flower water, which is stored refrigerated.

[0044] 4. Preparation of Jasminum officinale L. flower water

[0045] (1) Harvesting: On a sunny and dry day during the flowering period, fully opened flowers are picked, cut with sterilized scissors, placed in a breathable container, and transported as soon as possible.

[0046] (2) Cleaning and sorting: Stir and wash with clean water, remove impurities with tweezers, and blot dry with a paper towel.

[0047] (3) Extraction: By water distillation method. The flowers are put into a distillation pot, and (6-10) times the mass of distilled water is added. The initial distillate of 1-2L is obtained by distillation, and subsequent distillation continues.

[0048] (4) Mixing and purification: The initial distillate is mixed with the subsequent distillates, adsorbed with 0.5%-1% activated carbon, and filtered through a 0.22μm microporous filter membrane to obtain purified flower water, which is sealed in a brown bottle and stored in the dark at low temperature.

[0049] Poria cocos polysaccharide, natto gum, and snail mucus extract are products purchased directly.

[0050] Poria cocos polysaccharide is purchased from Shanxi Lanyuan Biotechnology Co., Ltd., with the product number LYSW-0824-326.

[0051] Natto gum is purchased from Shaanxi Boundary Biotechnology Co., Ltd., with the product number BJ-30.

[0052] Snail mucus extract is purchased from Shanxi Lanyuan Biotechnology Co., Ltd., with the product number LYSW-0706-007.

[0053] Elastin uses the recombinant elastin disclosed in Chinese Patent CN117551184B.

[0054] Example 1

[0055] A sterile plant flower water stock solution comprises the following components in parts by mass: 15 parts of Strongylodon macrobotrys flower water, 15 parts of Couroupita guianensis flower water, 15 parts of Strelitzia reginae flower water, 15 parts of Jasminum officinale L. flower water, 1.0 part of Poria cocos polysaccharide, 0.05 part of natto gum, 7.95 parts of snail mucus extract, and 1.0 part of elastin.

[0056] The preparation method is as follows:

[0057] Step 1, raw material mixing and dissolution: Add jade vine flower water, cannonball flower water, bird of paradise flower water, jasmine water, poria cocos polysaccharide, sodium alginate, snail mucus extract and elastin into the emulsifying pot in sequence. Stir and heat at a temperature of 80 °C, set the stirring speed at 150 r / min, and conduct homogenization treatment for 2 min at the same time. Then keep stirring for 15 min to ensure that all materials are fully dissolved.

[0058] Step 2, filtration and filling: After dissolution is completed, take samples for inspection. The qualified materials are filtered through a 200-mesh filter cloth into the material container of the filling equipment, and then hot filling and capping are carried out.

[0059] Step 3, sterilization and leak detection: Sterilize the filled product at 121 °C under high temperature and high humidity for 15 minutes, and conduct leak detection by the Quality Department. It can be discharged only after passing the inspection.

[0060] Example 2

[0061] Stock solution formula: A sterile plant flower water stock solution contains the following components in parts by mass: 5 parts of jade vine flower water, 30 parts of cannonball flower water, 10 parts of bird of paradise flower water, 30 parts of jasmine water, 0.05 part of poria cocos polysaccharide, 0.03 part of sodium alginate, 1 part of snail mucus extract, and 13.92 parts of elastin.

[0062] Preparation steps

[0063] Raw material mixing and dissolution: Add jade vine flower water, cannonball flower water, bird of paradise flower water, jasmine water, poria cocos polysaccharide, sodium alginate, snail mucus extract and elastin into the emulsifying pot in sequence. Stir and heat at a temperature of 82 °C, set the stirring speed at 180 r / min, and conduct homogenization treatment for 3 min at the same time. Then keep stirring for 20 min to ensure that all materials are fully dissolved.

[0064] Filtration and filling: After dissolution is completed, take samples for inspection. The qualified materials are filtered through a 200-mesh filter cloth into the material container of the filling equipment, and then hot filling and capping are carried out.

[0065] Sterilization and leak detection: Sterilize the filled product at 121 °C under high temperature and high humidity for 15 minutes, and conduct leak detection by the Quality Department. It can be discharged only after passing the inspection.

[0066] Example 3

[0067] Stock solution formula: A sterile plant flower water stock solution contains the following components in parts by mass: 30 parts of jade vine flower water, 5 parts of cannonball flower water, 30 parts of bird of paradise flower water, 5 parts of jasmine water, 0.2 part of poria cocos polysaccharide, 0.05 part of sodium alginate, 10 parts of snail mucus extract, and 0.75 part of elastin.

[0068] Preparation steps

[0069] Raw material mixing and dissolution: Add jade vine flower water, cannonball flower water, bird of paradise flower water, jasmine water, poria cocos polysaccharide, sodium alginate, snail mucus extract, and elastin into the emulsifying pot in sequence. Stir and heat at a temperature of 85°C, set the stirring speed at 200 r / min, conduct homogenization treatment for 5 min simultaneously, and then keep stirring for 30 min to ensure that all materials are fully dissolved.

[0070] Filtration and filling: After dissolution is completed, take samples for inspection. The qualified materials are filtered through a 200-mesh filter cloth into the material container of the filling equipment, and then hot-filled and capped.

[0071] Sterilization and leak detection: Sterilize the filled product at 121°C under high-temperature and high-humidity conditions for 15 minutes, and conduct leak detection by the Quality Department. Only the qualified products can be discharged.

[0072] Example 4

[0073] Stock solution formula: A sterile plant flower water stock solution, containing the following components in parts by mass: 20 parts of jade vine flower water, 20 parts of cannonball flower water, 10 parts of bird of paradise flower water, 20 parts of jasmine water, 0.1 part of poria cocos polysaccharide, 0.04 part of sodium alginate, 5 parts of snail mucus extract, and 4.86 parts of elastin.

[0074] Preparation steps

[0075] Raw material mixing and dissolution: Add jade vine flower water, cannonball flower water, bird of paradise flower water, jasmine water, poria cocos polysaccharide, sodium alginate, snail mucus extract, and elastin into the emulsifying pot in sequence. Stir and heat at a temperature of 83°C, set the stirring speed at 170 r / min, conduct homogenization treatment for 4 min simultaneously, and then keep stirring for 25 min to ensure that all materials are fully dissolved.

[0076] Filtration and filling: After dissolution is completed, take samples for inspection. The qualified materials are filtered through a 200-mesh filter cloth into the material container of the filling equipment, and then hot-filled and capped.

[0077] Sterilization and leak detection: Sterilize the filled product at 121°C under high-temperature and high-humidity conditions for 15 minutes, and conduct leak detection by the Quality Department. Only the qualified products can be discharged.

[0078] Example 5

[0079] Stock solution formula: A sterile plant flower water stock solution, containing the following components in parts by mass: 10 parts of jade vine flower water, 10 parts of cannonball flower water, 25 parts of bird of paradise flower water, 10 parts of jasmine water, 0.15 part of poria cocos polysaccharide, 0.02 part of sodium alginate, 3 parts of snail mucus extract, and 6.83 parts of elastin.

[0080] Preparation steps

[0081] Raw material mixing and dissolution: Add jade vine flower water, cannonball flower water, bird-of-paradise flower water, jasmine water, poria cocos polysaccharide, sodium alginate, snail mucus extract and elastin into the emulsifying pot in sequence. Stir and heat at a temperature of 81°C, set the stirring speed at 160 r / min, and conduct homogenization treatment for 3 min at the same time. Then keep stirring for 22 min to ensure that all materials are fully dissolved.

[0082] Filtration and filling: After dissolution is completed, take samples for inspection. The qualified materials are filtered through a 200-mesh filter cloth into the material container of the filling equipment, and then hot-filled and capped.

[0083] Sterilization and leak detection: Sterilize the filled product at a high temperature and humidity of 121°C for 15 minutes, and conduct leak detection by the Quality Department. It can only be discharged after passing the inspection.

[0084] Comparative Example 1

[0085] Formula: By mass, it includes: 60 parts of jade vine flower water, 1.0 part of poria cocos polysaccharide, 0.05 part of sodium alginate, 7.95 parts of snail mucus extract, and 1.0 part of elastin.

[0086] Preparation method: The same as the preparation method of Example 1. Add each raw material into the emulsifying pot, stir and heat, keep stirring, homogenize, filter, fill, sterilize and conduct leak detection.

[0087] Comparative Example 2

[0088] Formula: By mass, it includes: 60 parts of jasmine water, 1.0 part of poria cocos polysaccharide, 0.05 part of sodium alginate, 7.95 parts of snail mucus extract, and 1.0 part of elastin.

[0089] Preparation method: The same as the preparation method of Example 1. Add each raw material into the emulsifying pot, stir and heat, keep stirring, homogenize, filter, fill, sterilize and conduct leak detection.

[0090] Comparative Example 3

[0091] Formula: By mass, it includes: 20 parts of jade vine flower water, 20 parts of cannonball flower water, 1.0 part of poria cocos polysaccharide, 0.05 part of sodium alginate, 7.95 parts of snail mucus extract, and 1.0 part of elastin, without bird-of-paradise flower water and jasmine water.

[0092] Preparation method: The same as the preparation method of Example 2. Add each raw material into the emulsifying pot, stir and heat, keep stirring, homogenize, filter, fill, sterilize and conduct leak detection.

[0093] Comparative Example 4

[0094] Formula: By mass parts, it includes: 30 parts of Strelitzia reginae flower water, 30 parts of Jasminum officinale flower water, 1.0 part of pachyman, 0.05 part of sodium alginate, 7.95 parts of snail mucus extract, 1.0 part of elastin, without Strongylodon macrobotrys flower water and Couroupita guianensis flower water.

[0095] Preparation method: The same as the preparation method of Example 1. Add each raw material to an emulsifying pot, stir and heat, keep warm and stir, homogenize, filter, fill, sterilize and leak check.

[0096] Verification experiment on the synergistic anti-inflammatory effect of flower water

[0097] 1. Experimental purpose: To deeply explore the alleviating effect of the combined use of Strongylodon macrobotrys flower water, Couroupita guianensis flower water, Strelitzia reginae flower water and Jasminum officinale flower water on skin inflammation and its mechanism of action.

[0098] 2. Experimental materials

[0099] (1) Experimental animals: 60 6-week-old SPF-grade female Balb / c mice with a body weight between 18 - 22 g were selected to ensure the consistency and health status of the experimental subjects.

[0100] (2) Experimental reagents: Prepare a 1% dinitrofluorobenzene (DNFB) solution to induce skin inflammation in mice; purchase high-purity and high-activity Strongylodon macrobotrys flower water, Couroupita guianensis flower water, Strelitzia reginae flower water, and Jasminum officinale flower water from reliable plant flower water raw material suppliers; prepare 0.9% normal saline as a blank control reagent; select dexamethasone cream as a positive control drug.

[0101] (3) Experimental instruments: Prepare an electronic balance for accurately weighing reagents and drugs; a vernier caliper for measuring changes in the skin thickness of mice; a pipette for accurately sucking and dispensing reagents; a sterile syringe for drug injection and skin application.

[0102] 3. Experimental steps

[0103] (1) Animal grouping and adaptive feeding: Randomly divide 60 mice into 6 groups with 10 mice in each group. The grouping is as follows: normal control group, model control group, Strongylodon macrobotrys flower water group (comparative example 1), Couroupita guianensis flower water group (comparative example 2), Strelitzia reginae flower water group (comparative example 3), Strongylodon macrobotrys flower water + Couroupita guianensis flower water combination group (comparative example 4), Strelitzia reginae flower water + Jasminum officinale flower water combination group, a 1:1:1:1 mixture of four flower waters (Example 1), positive control group (dexamethasone cream group). Place the mice in an environment with a temperature of (22 ± 2) °C and a relative humidity of (50 ± 5) % for adaptive feeding for 1 week. During this period, the mice can freely eat and drink to ensure that the mice adapt to the experimental environment.

[0104] (2) Construction of skin inflammation model: After the adaptive feeding period, for all mice except those in the normal control group, use an 8% sodium sulfide solution to depilate an area of approximately 2 cm × 2 cm on their backs. Ensure that the solution covers evenly during the operation to achieve an ideal depilation effect. On the day after depilation, use a pipette to aspirate 20 μL of a 1% DNFB solution and evenly coat it on the depilated area of the mouse's back to complete the sensitization operation. On the 5th and 7th days after sensitization, use a pipette to aspirate 10 μL of a 1% DNFB solution again and precisely apply it to the same part of the mouse to successfully induce a skin inflammation model. Mice in the normal control group are smeared with an equal amount of physiological saline at the corresponding time points.

[0105] (3) Drug treatment: After inflammation is induced, mice in the normal control group and the model control group are smeared with an equal amount of physiological saline; the jade vine flower water group is smeared with the sample prepared in Comparative Example 1 in equal amount, the cannonball flower water group is smeared with the sample prepared in Comparative Example 2, the strelitzia flower water group is smeared with the sample prepared in Comparative Example 3, the jade vine flower water + cannonball flower water combination group is smeared with the sample prepared in Comparative Example 4, and the strelitzia flower water + jasminum officinale flower water combination group (a 1:1:1:1 mixture of the four flower waters) is smeared with the sample prepared in Example 1; the positive control group is smeared with an appropriate amount of dexamethasone cream. Apply once a day for 7 consecutive days. The application volume or dose is determined according to the mouse body weight and the results of the preliminary experiment to ensure that the application amount of each group is consistent and will not cause discomfort to the mice.

[0106] 4. Observation indicators and detection methods

[0107] (1) Measurement of skin thickness: On the 0th, 3rd, and 7th days of the experiment, use a vernier caliper to measure the skin thickness of the inflamed area on the mouse's back, record and calculate the average value and standard deviation of each group to evaluate the skin edema changes caused by inflammation.

[0108] (2) Scoring of skin inflammation symptoms: The following scoring criteria are used to evaluate the inflammation symptoms:

[0109]

[0110]

[0111] (3) Detection of inflammatory factors: On the 7th day of the experiment, collect the skin tissue of the inflamed area of the mouse, and detect the contents of inflammatory factors such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by enzyme-linked immunosorbent assay (ELISA) technology to deeply understand the effect of flower water on the secretion of inflammatory factors.

[0112] 5. Experimental results

[0113] (1) Changes in skin thickness

[0114]

[0115] From the changes in skin thickness, it can be seen that in the model control group of mice, due to inflammation, the skin thickness increased from the initial 0.54±0.03 mm to 0.85±0.05 mm on the 7th day. After using the aqueous extract of Strongylodon macrobotrys flowers, the aqueous extract of Couroupita guianensis flowers, and the aqueous extract of Strelitzia reginae flowers alone, the skin thickness on the 7th day decreased to 0.65±0.04 mm, 0.67±0.04 mm, and 0.66±0.04 mm respectively. The decrease in the mixed group of the four flower extracts was the largest, reaching 0.59±0.03 mm, which was significantly different from the single group (P<0.05). The skin thickness of the combination group of Strongylodon macrobotrys flower water + Couroupita guianensis flower water on the 7th day was 0.66±0.04 mm, and the skin thickness of the combination group of Strelitzia reginae flower water + Jasminum officinale var. grandiflorum flower water on the 7th day was 0.64±0.04 mm, both of which were greater than that of the mixed group of the four flower extracts, indicating that the mixed group of the four flower extracts had obvious advantages in reducing inflammatory edema.

[0116] (2) Skin inflammation symptom scores are shown in the following table:

[0117] Group Score Normal control group 3 points Model control group 2 points Strongylodon macrobotrys flower water group 2 points Couroupita guianensis flower water group 2 points Strelitzia reginae flower water group 2 points Combination group of Strongylodon macrobotrys flower water + Couroupita guianensis flower water 2 points Combination group of Strelitzia reginae flower water + Jasminum officinale var. grandiflorum flower water 2 points Positive control group (dexamethasone cream group) 2 points Mixture group of four kinds of flower water in a ratio of 1:1:1:1 0 points

[0118] The skin inflammation reaction of the mice in the model control group was severe and the scores were high. When using the aqueous extract of Strongylodon macrobotrys flowers, the aqueous extract of Couroupita guianensis flowers, and the aqueous extract of Strelitzia reginae flowers alone, there was a certain degree of relief for the inflammatory symptoms. The improvement of the inflammatory symptoms of the mice in the mixed group of the four flower extracts was the most significant, and the scores were significantly lower than those of the single group (P<0.05). The scores of the combination group of Strongylodon macrobotrys flower water + Couroupita guianensis flower water and the combination group of Strelitzia reginae flower water + Jasminum officinale var. grandiflorum flower water were higher than those of the mixed group of the four flower extracts, indicating that the four extracts could work together to more effectively reduce the external manifestations of inflammation.

[0119] (3) Detection results of inflammatory factors

[0120]

[0121] In this experiment, the mice in the model control group showed severe skin inflammatory reactions, and the levels of TNF-α (56.8±5.2 pg / mL) and IL-6 (45.6±4.8 pg / mL) increased significantly. In contrast, when using the aqueous extract of Strongylodon macrobotrys flowers, the aqueous extract of Couroupita guianensis flowers, and the aqueous extract of Strelitzia reginae flowers alone, there was a certain degree of relief for the inflammatory symptoms, and the levels of inflammatory factors in each flower water group decreased to varying degrees. Among them, the improvement of the inflammatory symptoms of the mice in the 1:1:1:1 mixed group of the four flower extracts was the most significant, and the levels of TNF-α (25.6±3.0 pg / mL) and IL-6 (20.5±2.5 pg / mL) were significantly lower than those of the single group (P<0.05). The combination group of Strongylodon macrobotrys flower water + Couroupita guianensis flower water and the combination group of Strelitzia reginae flower water + Jasminum officinale var. grandiflorum flower water also had certain effects in reducing the levels of inflammatory factors, but their scores were higher than those of the mixed group of the four flower extracts, indicating that the four extracts could work together to more effectively reduce the external manifestations of inflammation. The experimental results are analyzed below in combination with the main components of the four flower extracts.

[0122] Main components and anti-inflammatory mechanisms of each flower water:

[0123] (1) Jade vine flower water: Jade vine flowers are rich in flavonoids. Flavonoids have a wide range of biological activities, and their anti-inflammatory mechanisms mainly include inhibiting the nuclear factor-κB (NF-κB) signaling pathway in inflammatory cells, reducing the transcription and synthesis of inflammatory factors such as TNF-α and IL-6; at the same time, the antioxidant properties of flavonoids can scavenge excessive reactive oxygen species (ROS) in the body, reduce the damage of oxidative stress to cells, and indirectly inhibit the inflammatory response. In this experiment, TNF-α in the jade vine flower water group decreased to 38.5 ± 4.1 pg / mL, and IL-6 decreased to 30.2 ± 3.5 pg / mL, indicating its certain efficacy in alleviating inflammation.

[0124] (2) Cannonball flower water: Cannonball flowers contain terpenoids. Terpenoids can exert anti-inflammatory effects through various pathways, such as inhibiting the chemotaxis and aggregation of inflammatory cells, reducing the release of inflammatory mediators; regulating the activity of inflammation-related enzymes, such as inhibiting the expression of cyclooxygenase-2 (COX-2), thereby reducing the synthesis of pro-inflammatory substances such as prostaglandins. In the experiment, TNF-α in the cannonball flower water group was 36.7 ± 3.8 pg / mL, and IL-6 was 28.9 ± 3.2 pg / mL, reflecting its inhibitory effect on inflammation.

[0125] (3) Bird-of-paradise flower water: Bird-of-paradise flowers contain phenolic compounds. Phenolic components have strong antioxidant and anti-inflammatory activities, and can inhibit the transduction of inflammatory signals by regulating the intracellular redox state. Specifically, phenolic substances can inhibit the activation of the mitogen-activated protein kinase (MAPK) signaling pathway, reducing the production and release of inflammatory factors. TNF-α in the bird-of-paradise flower water group decreased to 35.4 ± 3.6 pg / mL, and IL-6 decreased to 27.8 ± 3.0 pg / mL, indicating its positive effect on alleviating inflammatory symptoms.

[0126] (4) Arabian jasmine flower water: Arabian jasmine flowers contain volatile oils and other components. The active ingredients in volatile oils can act on cell membranes, regulate the fluidity and permeability of cell membranes, and affect the intracellular signal transduction process. At the same time, volatile oils also have antibacterial and anti-inflammatory effects, and may reduce the inflammatory response by inhibiting the activation of inflammatory cells and the release of inflammatory mediators. In the bird-of-paradise flower water + Arabian jasmine flower water combination group, TNF-α decreased to 32.8 ± 3.6 pg / mL, and IL-6 decreased to 25.9 ± 3.0 pg / mL, showing the synergistic inhibitory effect on inflammation after the combination of Arabian jasmine flower water and bird-of-paradise flower water.

[0127] Synergistic anti-inflammatory effect of the mixture of four flower waters

[0128] When the four kinds of flower waters are mixed in a ratio of 1:1:1:1, various components such as flavonoids, terpenoids, phenols, and volatile oils contained in them synergize with each other to exert a stronger anti-inflammatory effect. Different types of components intervene in the inflammatory response from multiple levels and targets, forming a complex and efficient anti-inflammatory network. For example, flavonoid compounds inhibit the NF-κB signaling pathway, terpenoid substances regulate the activity of inflammation-related enzymes, phenolic components regulate the redox state, and volatile oils affect cell signal transduction. These effects complement and enhance each other, making the mixed group more prominent in reducing the levels of TNF-α and IL-6, thereby significantly improving the inflammatory symptoms of mice. This synergistic effect is the key reason why the improvement degree of the inflammatory symptoms in the mixed group is better than that in the single group and the pairwise combination groups.

[0129] In summary, due to the differences in the main components contained in different flower waters, there are certain differences in their anti-inflammatory effects. After the four flower waters are mixed, the synergistic effect of their components can more comprehensively and effectively inhibit the inflammatory response, providing a potential research direction for the development of new functional skin care products.

[0130] The capacitance moisture tester detects the moisture retention

[0131] 1. Preparation:

[0132] (1) Instrument calibration: Turn on the capacitance moisture tester and calibrate the instrument using a standard sample to ensure the accuracy of the measurement data. At the same time, prepare skin inflammation detection equipment, a skin erythema tester, for detecting skin inflammation-related indicators.

[0133] (2) Skin cleaning: Select 120 volunteers and randomly divide them into twelve groups, with 10 people in each group.

[0134] For the first group, use the stock solution of Example 1 on the left cheek and the stock solution of Comparative Example 1 on the right cheek;

[0135] For the second group, use the stock solution of Example 1 on the left cheek and the stock solution of Comparative Example 2 on the right cheek;

[0136] For the third group, use the stock solution of Example 2 on the left cheek and the stock solution of Comparative Example 1 on the right cheek;

[0137] For the fourth group, use the stock solution of Example 2 on the left cheek and the stock solution of Comparative Example 2 on the right cheek;

[0138] For the fifth group, use the stock solution of Example 3 on the left cheek and the stock solution of Comparative Example 1 on the right cheek;

[0139] For the sixth group, use the stock solution of Example 3 on the left cheek and the stock solution of Comparative Example 2 on the right cheek;

[0140] For the seventh group, use the stock solution of Example 4 on the left cheek and the stock solution of Comparative Example 1 on the right cheek;

[0141] Group 8: Use the stock solution of Example 4 on the left cheek and the stock solution of Comparative Example 2 on the right cheek;

[0142] Group 9: Use the stock solution of Example 5 on the left cheek and the stock solution of Comparative Example 1 on the right cheek;

[0143] Group 10: Use the stock solution of Example 5 on the left cheek and the stock solution of Comparative Example 2 on the right cheek;

[0144] Group 11: Use the stock solution of Comparative Example 3 on the left cheek and the stock solution of Comparative Example 4 on the right cheek;

[0145] Group 12 serves as a blank control without using any test products.

[0146] Clean the facial skin of the volunteers with a mild amino acid facial cleanser, press dry the moisture, and sit quietly in an air-conditioned room at a temperature of 24°C and a relative humidity of 50% for 30 minutes to allow the skin to return to its natural state.

[0147] 2. Measurement process:

[0148] (1) Initial measurement: Gently place the probe of the capacitive moisture tester vertically on the facial skin, ensuring that the probe is in complete contact with the skin without air gaps, and record the skin moisture content data at this time as the basic moisture value before using the product. At the same time, use a skin erythema tester to detect skin inflammation-related indicators such as erythema intensity and area, and record the initial inflammation data.

[0149] (2) Product application and waiting: Evenly apply the stock solutions of the examples and comparative examples to the designated areas of the face, gently spread them evenly in the test areas, and gently massage with the fingertips until the products are completely absorbed by the skin. At 30 minutes, 1 hour, 4 hours, 8 hours, 12 hours, and 24 hours after application, use the capacitive moisture tester to measure the skin moisture content; at 12 hours and 24 hours after application, use the skin erythema tester to detect the skin inflammation-related indicators again to observe the improvement of inflammation.

[0150] 3. Test results:

[0151] (1) Proportion of change in moisture content

[0152]

[0153]

[0154] The proportion of increase in moisture content of Examples 1-5 is significantly better than that of the comparative examples, showing a significant difference in statistical significance (P < 0.05). This indicates that the sterile plant flower water stock solutions prepared with different formulations of the present invention all have significant moisturizing effects.

[0155] (2) Improvement of skin inflammation

[0156]

[0157]

[0158] Examples 1-5 were significantly superior to Comparative Examples 1-4 in terms of reducing erythema intensity and erythema area, showing significant differences in statistical significance (P < 0.05). This indicates that the sterile plant flower water stock solutions with different formulations of the present invention all have outstanding effects in improving skin inflammation.

[0159] The subjects reported that the stock solutions of Examples 1-5 had appropriate viscosities, good skin feelings, fast absorption after application on the face, no greasiness, and strong residual feelings, and there were no allergies or adverse reactions after use. This is due to the selected natural source components and the hot and humid sterilization technology adopted, which not only ensure the sterility of the final product but also ensure the safety of the product.

[0160] In summary, through a variety of unique plant flower water combinations and innovative preparation methods, the present invention has developed a sterile plant flower water stock solution with excellent moisturizing and anti-inflammatory effects, providing a safe and effective skin care option for people with sensitive skin.

[0161] Where not covered above, the prior art applies.

[0162] Although some specific embodiments of the present invention have been described in detail by way of examples, those skilled in the art should understand that the above examples are only for illustration and not for limiting the scope of the present invention. Those skilled in the technical field to which the present invention pertains can make various modifications or supplements to the described specific embodiments or use similar ways for substitution, but will not deviate from the direction of the present invention or exceed the scope defined by the appended claims. Those skilled in the art should understand that any modifications, equivalent substitutions, improvements, etc. made according to the technical essence of the present invention to the above embodiments should be included in the protection scope of the present invention.

Claims

1. A sterile plant flower water stock solution, characterized in that, It comprises raw materials in the following parts by mass: 5 - 30 parts of jade vine flower water, 5 - 30 parts of cannonball tree flower water, 5 - 30 parts of bird of paradise flower water, 5 - 30 parts of jasmine water, 0.05 - 1 part of pachymaran, 0.01 - 0.05 part of natto gum, 1 - 10 parts of snail mucus extract and 0.75 - 14 parts of elastin.

2. The sterile plant flower water stock solution according to claim 1, characterized in that, The preparation method of jade vine flower water is as follows: Gently wash and crush the picked fresh jade vine flowers, and then use the steam distillation method to collect the distillate to obtain crude flower water; Filter, centrifuge and finely filter the crude flower water to obtain refined jade vine flower water.

3. The aseptic plant flower water stock solution according to claim 1, characterized in that, The preparation method of cannonball tree flower water is as follows: Wash, disinfect and air-dry the fresh, mature and intact cannonball tree flowers, then use supercritical carbon dioxide extraction to obtain a cannonball tree flower extract, and then dilute the extract with sterile deionized water at a ratio of 1:10 - 20 and filter to obtain sterile cannonball tree flower water.

4. The sterile plant flower water stock solution according to claim 1, wherein The preparation method of bird of paradise flower water is as follows: Wash and cut the picked fresh bird of paradise flowers into sections, then use an ethanol aqueous solution with a concentration of 40% - 60% as the extraction solvent for extraction, then filter and evaporate the extract, and then dialyze and filter the concentrated solution to remove alcohol to obtain refined bird of paradise flower water.

5. The sterile plant flower water stock solution according to claim 1, characterized in that, The preparation method of jasmine water is as follows: Wash the picked fresh jasmine flowers and remove the surface moisture, then use the water distillation method to obtain 1 - 2 L of the initial distillate, and continue distillation; Mix the initial distillate with the subsequent distillate, adsorb with activated carbon and then filter to obtain refined jasmine water.

6. A method for preparing the sterile plant flower water stock solution according to any one of claims 1-5, characterized in that: Add jade vine flower water, cannonball tree flower water, bird of paradise flower water, jasmine water, pachymaran, natto gum, snail mucus extract and elastin into an emulsifying pan in the following parts by mass in sequence, heat and stir, then perform homogenization treatment, and then keep stirring while heating until all the materials are fully dissolved, and then perform moist heat sterilization to obtain the sterile plant flower water stock solution.

7. The preparation method according to claim 6, characterized in that: The temperature for heating and stirring is 80 - 85 °C, and the stirring speed is 150 - 200 r / min.

8. The preparation method according to claim 6, characterized in that: Perform homogenization treatment for 2 - 5 min; Keep stirring while heating for 15 - 30 min.

9. The preparation method according to claim 6, characterized in that: After all the materials are fully dissolved, sample and test according to the "Technical Specifications for Cosmetics Safety". The qualified material is filtered through a 200-mesh filter cloth into the material container of the filling equipment, and then hot-filled and capped; Finally, the filled product is sterilized by high-temperature moist heat to obtain the sterile plant flower water stock solution.

10. Use of the sterile plant flower water stock solution according to any one of claims 1-5, characterized in that: It is used in skin care products for moisturizing and anti-inflammatory of sensitive skin.

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