A soothing composition and uses thereof, a skin care product

Abstract

This invention belongs to the field of cosmetic technology and discloses a soothing composition and its uses, as well as skincare products. The soothing composition is obtained by combining extracts of Ophiopogon japonicus root, dandelion, and oregano leaf. The extracts are obtained by alcohol extraction of the plant combination of Ophiopogon japonicus root, dandelion, and oregano leaf, wherein the weight ratio of Ophiopogon japonicus root, dandelion, and oregano leaf in the plant combination is 2-10:2-10:1-8. When the three extracts are combined, they can intervene in the inflammatory response from multiple angles to reduce inflammation and achieve a soothing effect. The three components also work together to eliminate more types of free radicals and enhance the overall free radical scavenging efficiency through synergistic effects. Furthermore, the moisturizing components of the three components can complement each other to build a more complete moisturizing system. The three components have a strong synergistic effect in anti-inflammatory, antioxidant, and moisturizing aspects, thereby achieving a superior soothing effect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and discloses a soothing composition and its uses, as well as skin care products. Background Technology

[0002] With changes in dietary structure, environmental pollution, and increasing work and life pressures, skin is exposed to various complex and harmful environmental factors, posing an unprecedented challenge to skin health. Excessive ultraviolet radiation not only leads to sunburn and tanning, but long-term accumulation can also cause skin aging, pigmentation, and increase the risk of skin cancer. At the same time, increasingly severe air pollution, containing particulate matter and chemical pollutants, easily adheres to the skin surface, clogging pores, disrupting the skin's normal metabolic function, and leading to problems such as acne, pimples, and dullness. Furthermore, with the development of today's society, people's pursuit of beauty is increasingly high, and in many occasions, makeup is needed to present one's best self. However, the use of various cosmetics also poses potential threats to the skin. Many cosmetics contain chemical ingredients such as alcohol, fragrances, and preservatives, which may irritate the skin, causing discomfort such as allergies, itching, and redness.

[0003] Therefore, cosmetics on the market often add soothing ingredients to alleviate various skin problems. However, traditional soothing products generally have the following problems: First, they do not provide deep enough relief. Most existing soothing cosmetics only focus on immediate surface symptom relief. For example, adding cooling ingredients such as menthol can create a cooling sensation on the skin upon application, temporarily relieving the burning and stinging caused by sunburn or allergies. However, this method only numbs the nerve endings in the skin and does not repair the root cause of skin damage. Second, many soothing products have unreasonable formulations, with the ingredients failing to work synergistically and only providing a single effect.

[0004] Therefore, the technical problem that this invention needs to solve is how to prepare a soothing product with highly effective soothing effects. Summary of the Invention

[0005] The purpose of this invention is to provide a soothing composition containing Ophiopogon japonicus root extract, dandelion extract and oregano leaf extract. When used together, the three components have a synergistic effect and have an excellent soothing effect.

[0006] In addition, the present invention also provides the use of the soothing composition and skin care products.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] A soothing composition comprising extracts of Ophiopogon japonicus root, dandelion and oregano leaf, wherein the extracts are obtained by alcohol extraction of the plant combination of Ophiopogon japonicus root, dandelion and oregano leaf, wherein the weight ratio of Ophiopogon japonicus root, dandelion and oregano leaf in the plant combination is 2-10:2-10:1-8.

[0009] The substances used in this invention have the following effects:

[0010] Ophiopogon japonicus root extract

[0011] In terms of antioxidants: Ophiopogon japonicus root extract contains antioxidants such as flavonoids and saponins. These components can react with free radicals through their phenolic hydroxyl groups to generate relatively stable phenoxy radicals, preventing further free radical chain reactions, reducing oxidative stress damage to the skin, and alleviating skin inflammation and discomfort caused by oxidative damage, thus soothing the skin. In addition, Ophiopogon japonicus root extract can regulate the antioxidant enzyme system in the skin, promoting the synthesis of antioxidant enzymes or enhancing their activity, helping to maintain the redox balance within skin cells, reducing skin inflammation caused by redox imbalance, and achieving a soothing effect on the skin.

[0012] In terms of anti-inflammation: Ophiopogon japonicus root extract can inhibit the activation of inflammatory cells, thereby reducing the release of inflammatory mediators. Some components in Ophiopogon japonicus root extract may act on the signaling pathways of mast cells, preventing their degranulation process and reducing the release of histamine, thus alleviating skin allergy and inflammation symptoms and exerting a soothing effect. At the same time, Ophiopogon japonicus root extract can regulate the function of immune cells, inhibiting inflammatory responses by regulating the secretion of cytokines, and thus soothing the skin.

[0013] Dandelion extract

[0014] In terms of antioxidant properties: the polyphenols and flavonoids in dandelion extract are powerful free radical scavengers. These compounds have multiple phenolic hydroxyl groups, and the hydrogen atoms on these hydroxyl groups can be easily removed by free radicals. In particular, they are effective against superoxide anion radicals (O2). - When flavonoids react with hydroxyl radicals (·OH), the hydrogen atoms in polyphenolic compounds combine with the free radicals to form relatively stable free radical intermediates. Due to the presence of conjugated structures such as benzene rings, these intermediates can disperse the energy of unpaired electrons through resonance and other mechanisms, increasing their stability and thus preventing the chain reaction of free radicals. Flavonoid free radicals can delocalize electrons through intramolecular hydrogen bonds and conjugated systems, increasing their stability and effectively scavenging free radicals, reducing oxidative damage to skin cells, and achieving a soothing effect.

[0015] In terms of anti-inflammation: Dandelion extract is rich in flavonoids, which can inhibit key enzymes in the inflammatory response. Dandelion extract can also inhibit nitric oxide (NO) synthesis. In inflammatory cells (such as macrophages), inducible nitric oxide synthase (iNOS) is activated under inflammatory stimulation, producing large amounts of NO. NO has effects such as vasodilation, increased vascular permeability, and participation in inflammatory cytotoxicity. Dandelion extract may reduce NO synthesis by inhibiting iNOS gene expression or interfering with its enzyme activity, thereby alleviating the inflammatory response. Dandelion extract can also regulate eosinophil function. In allergic inflammation and some inflammatory skin diseases, eosinophils are recruited to the site of inflammation, releasing various toxic proteins and inflammatory mediators. Dandelion extract may reduce skin inflammation by regulating the expression of cell surface receptors or intracellular signal transduction, inhibiting eosinophil activation and the release of inflammatory mediators.

[0016] Oregano leaf extract

[0017] The polysaccharide components in oregano leaf extract play a role in skin moisturizing. These polysaccharide molecules have a large number of hydroxyl groups, which can form hydrogen bonds with water molecules. When applied to the skin surface, the polysaccharide molecules intertwine to form a moisturizing film on the surface of the stratum corneum. This moisturizing film can prevent the loss of moisture from the skin's interior, reducing transepidermal water loss (TEWL). Furthermore, this moisturizing film can also act as a physical barrier, resisting the irritation of the skin by external environmental factors (such as dust, microorganisms, etc.). Simultaneously, the hydrophilicity of the polysaccharides allows them to absorb moisture from the surrounding environment, replenishing the skin's surface moisture and maintaining its hydration. In addition, oregano leaf extract can improve the arrangement and structure of stratum corneum cells. Under normal circumstances, the tightly packed and orderly arrangement of stratum corneum cells is conducive to moisture retention. Oregano leaf extract may improve the tighter arrangement of stratum corneum cells by regulating the expression of intercellular lipid components or cell junction proteins, thereby enhancing the stratum corneum's ability to retain moisture and relieving discomfort such as dry and tight skin.

[0018] When Ophiopogon japonicus root extract, dandelion extract, and oregano leaf extract are used simultaneously, a synergistic effect is observed. The underlying mechanism is speculated to be as follows: In terms of anti-inflammation, Ophiopogon japonicus root extract primarily works by regulating immune cell function, such as affecting T lymphocyte differentiation and Langerhans cell antigen presentation. Dandelion extract focuses on inhibiting the synthesis of inflammatory mediators (e.g., inhibiting enzymes related to the arachidonic acid metabolic pathway) and the activation of inflammatory cells (e.g., inhibiting mast cell degranulation). Oregano leaf extract alleviates inflammation by inhibiting the activity of key enzymes such as cyclooxygenase-2, lipoxygenase, and nitric oxide synthase through phenolic compounds. The combination of these three extracts can intervene in the inflammatory response from multiple angles. Furthermore, Ophiopogon japonicus root extract may inhibit the overexpression of inflammatory cytokines by regulating cytokine signaling; dandelion extract can inhibit inflammation-related signaling pathways such as NF-κB; and oregano leaf extract can also regulate the NF-κB signaling pathway to reduce the production of inflammatory mediators. When they act together, the regulation of these signaling pathways can produce a synergistic effect.

[0019] In terms of antioxidant properties, Ophiopogon japonicus root extract may be advantageous in scavenging certain types of free radicals (such as superoxide anion radicals), dandelion extract is effective at scavenging hydroxyl radicals, and oregano leaf extract excels at scavenging lipid peroxidation radicals. When they coexist, they can scavenge a wider range of free radicals and enhance overall free radical scavenging efficiency through synergistic effects. They may work together to form a relay-style free radical scavenging model, where the relatively stable intermediate products generated after one component scavenges free radicals can be further processed by another component, thereby more effectively interrupting the chain reaction of free radicals.

[0020] In terms of moisturizing, Ophiopogon japonicus root extract contains polysaccharides and other components that form a gel-like substance by binding with water molecules, creating a moisturizing film on the skin surface. Dandelion extract's polysaccharides can absorb and retain moisture, forming a moisturizing film in the stratum corneum and promoting the synthesis and distribution of natural moisturizing factor (NMF) within stratum corneum cells. Oregano leaf extract's polysaccharides also form a moisturizing film on the stratum corneum surface by forming hydrogen bonds with water molecules and regulating stratum corneum cell metabolism to promote the expression of NMF-related genes. The moisturizing components of these three extracts complement each other, creating a more complete moisturizing system. The gel-like moisturizing film formed by Ophiopogon japonicus root extract provides a basic level of hydration, while the polysaccharides in dandelion extract further enhance the water-retention capacity of the moisturizing film and promote NMF synthesis. Oregano leaf extract plays a role in regulating stratum corneum cell metabolism and enhancing the moisturizing barrier function, collectively reducing transepidermal water loss (TEWL) and increasing skin moisture content and hydration.

[0021] The three components have a synergistic effect in anti-inflammatory, antioxidant and moisturizing properties, which can achieve better soothing effects.

[0022] Preferably, the extraction method of the extract is as follows:

[0023] Step 1: Add 10 times the weight of the plant combination of Ophiopogon japonicus root, dandelion and oregano leaf to water, and extract at 65℃ for 3 hours to obtain an extract. Then concentrate the extract to 25-45 wt% of the extract to obtain the first concentrate. Add the same weight of 95 vol% ethanol to the first concentrate to obtain the alcohol precipitate.

[0024] Step 2: After centrifugation and filtration, the filtrate is concentrated until alcohol-free to obtain a second concentrate. Butylene glycol and water are added to the second concentrate, and the mixture is stirred and extracted for 0.5 hours. Ultrafiltration is then performed to obtain the ultrafiltrate, wherein the weight ratio of concentrate, butylene glycol, and water is 3-5:3:2-4.

[0025] Step 3: Add 0.5 wt% of 1,2-hexanediol and 0.5 wt% of p-hydroxyacetophenone to the filtrate, sterilize at 85°C for 1 hour to obtain the soothing composition.

[0026] Furthermore, the present invention also discloses the use of the above-mentioned soothing composition in the preparation of skin care products.

[0027] Preferably, the skin care product is a soothing skin care product.

[0028] Finally, the present invention also discloses a skin care product containing the above-mentioned soothing composition.

[0029] Preferably, the content of the soothing composition in the skin care product is 0.1 to 30 wt%.

[0030] Preferably, the dosage form of the skin care product is a lotion, cream, spray, serum, toner, mask, or gel.

[0031] Compared with the prior art, the beneficial effects of the present invention are:

[0032] The soothing composition of this invention contains Ophiopogon japonicus root extract, dandelion extract, and oregano leaf extract. When combined, these three ingredients intervene in the inflammatory response from multiple angles to reduce inflammation and achieve a soothing effect. Simultaneously, their synergistic effect helps to eliminate a wider range of free radicals and enhances the overall free radical scavenging efficiency. Furthermore, the moisturizing components of the three ingredients complement each other, creating a more comprehensive moisturizing system. The three components exhibit significant synergistic effects in anti-inflammatory, antioxidant, and moisturizing aspects, thereby achieving superior soothing efficacy. Attached Figure Description

[0033] Figure 1 This is an observation image of the chicken embryo before use in Example 1;

[0034] Figure 2This is an observation image of chicken embryos after use in Example 1. Detailed Implementation

[0035] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0036] Product Information:

[0037] Ophiopogon japonicus root: moisture content 14.3%, purchased from Bozhou Feimao Pharmaceutical Co., Ltd.;

[0038] Oregano leaves: moisture content 8.6%, purchased from Bozhou Feimao Pharmaceutical Co., Ltd.;

[0039] Dandelion: Moisture content 9.5%, purchased from Bozhou Feimao Pharmaceutical Co., Ltd.

[0040] The preparation methods of the soothing compositions in each embodiment and comparative example are as follows:

[0041] Step 1: Add 10 times the weight of the plant combination of Ophiopogon japonicus root, dandelion and oregano leaf to water, and extract at 65℃ for 3 hours to obtain an extract. Then concentrate the extract to 40wt% of the extract weight to obtain the first concentrate. Add the same weight of 95vol% ethanol to the first concentrate to obtain the alcohol precipitate.

[0042] Step 2: After centrifugation, concentrate the filtrate until alcohol-free to obtain a second concentrate. Add butanediol and water to the second concentrate, stir and extract for 0.5 h, and then perform ultrafiltration to obtain ultrafiltrate. The weight ratio of concentrate, butanediol and water is 4:3:3.

[0043] Step 3: Add 0.5 wt% of 1,2-hexanediol and 0.5 wt% of p-hydroxyacetophenone to the filtrate, sterilize at 85°C for 1 hour to obtain the soothing composition.

[0044] The proportions of the plant combinations in each embodiment and comparative example are shown in Table 1;

[0045] Table 1. Plant Combination Formula (parts by weight)

[0046] Ophiopogon japonicus Dandelion Oregano leaves Example 1 6 6 3 Example 2 10 2 8 Example 3 2 10 1 Comparative Example 1 15 0 0 Comparative Example 2 0 15 0 Comparative Example 3 0 0 15 Comparative Example 4 7.5 7.5 0 Comparative Example 5 10 0 5 Comparative Example 6 0 10 5

[0047] Performance testing

[0048] Chicken embryo chorionic allantoic membrane test

[0049] 1. Experimental Objective and Principle

[0050] The chorioallantoic membrane (CAM) test is an early and widely used in vitro method for assessing eye irritation. The CAM is a respiratory membrane surrounding the chicken embryo. This test utilizes the intact, clear, and transparent vascular system of the mid-stage chorioallantoic membrane in hatched chicken embryos. A certain amount of the test substance is directly exposed to the CAM, and after a period of time, changes in CAM toxicity indicators (such as hemorrhage, coagulation, and vascularization) are observed. These indicators reflect changes in the morphology, color, and permeability of blood vessels and vascular networks, as well as phenomena such as CAM protein denaturation and the degree of damage. These indicators are then combined to obtain a score used to assess the eye irritation of the test substance.

[0051] The purpose of this study is to test the ability of the test substance to cause toxic changes in the chorioallantoic membrane of chicken embryos and to evaluate the elements and processes of the potential eye irritation of the substance being evaluated.

[0052] 2. Experimental Materials and Methods

[0053] 2.1 Instruments and Equipment

[0054] Fully automatic incubator;

[0055] Stereo microscope;

[0056] SPF chicken embryos.

[0057] 2.2 Reagents

[0058] Sodium chloride;

[0059] Sodium dodecyl sulfate (SDS).

[0060] 2.3 Incubation conditions

[0061] Room temperature: 20–25℃, relative humidity: 45–70%. Incubation temperature: 37.5 ± 0.5℃, relative humidity: 55–70%, turn the tray 3–6 times / hour. Rotation is not necessary for 9-day-old chicken embryos during incubation.

[0062] 2.4 Test Methods

[0063] (1) Experimental operation procedures

[0064] In this test, 6 embryos were selected for each group. The condition of the chorioallantoic membrane was recorded using a photographic device. The composition of Example 1 was diluted with pure water to a sample concentration of 2% and then added to the chorioallantoic membrane of the chicken embryos. The time of sample addition was recorded. The air cell was covered with a moistened plastic wrap and the chicken embryos were transferred to a constant temperature and humidity chamber for culture. The degree of change of each toxic effect was observed and photographed.

[0065] (2) Results observation

[0066] Observe and record bleeding, coagulation, and vascularization manifestations, and score them according to their severity.

[0067] (3) Data Analysis

[0068] Table 2 Scoring Criteria

[0069]

[0070]

[0071] The endpoint evaluation method was used for the experiment, and the endpoint evaluation (ES) was calculated. The results were retained to two decimal places; ES≤4, no irritation; 4<ES≤12, mild irritation; 12<ES<16, moderate irritation; ES≥16, strong irritation / corrosiveness; the score for each chicken embryo was the sum of the observed bleeding, coagulation and vascularization in each chicken embryo; ES = the average of the mathematical sums obtained from 6 chicken embryos.

[0072] Testing showed that the composition of Example 1 was mild and non-irritating. (Reference) Figure 1 , Figure 2 The image shows a comparison of the chicken embryo's state before and after the solution prepared from the composition of Example 1 was added to the chicken embryo. Figure 1 This is an image of the chicken embryo before the sample was used. Figure 2 This is an observation image of chicken embryos after the sample was used.

[0073] Hyaluronidase inhibition rate

[0074] 1. Experimental Objective and Principle

[0075] Hyaluronidase is a specific enzyme that cleaves hyaluronic acid and is involved in allergic reactions, showing a strong correlation with histamine release from mast cells. The soothing effect of a test sample can be determined using the hyaluronidase inhibition rate; a higher inhibition rate indicates a stronger soothing effect, and vice versa.

[0076] This experiment compares the results of the hyaluronidase inhibition rate test between the test sample and the negative control. If the inhibition rate of the test sample is higher than that of the negative control, the test sample can be considered to have a certain soothing effect.

[0077] 2. Experimental Materials and Methods

[0078] 2.1 Instruments and Equipment

[0079] BSA224S analytical balance;

[0080] L6s UV spectrophotometer.

[0081] 2.2 Reagents

[0082] Hyaluronidase, purity BR;

[0083] Sodium hyaluronate, purity BR.

[0084] 2.3 Test Methods

[0085] (1) Treatment of control materials and test samples

[0086] Sample group: Dilute the compositions of Examples 1-3 and Comparative Examples 1-6 with pure water to a sample concentration of 5%;

[0087] Positive control (dipotassium glycyrrhizate, purity ≥98%): diluted with water to a positive control concentration of 3%;

[0088] Negative control: pure water.

[0089] (2) Experimental Operation Procedures

[0090] Set up a sample group, a sample background group, a solvent group, and a solvent background group. Each group should have 3 replicates. Add different reagent solutions to each of the four groups, shake well, and let stand at room temperature for 30 minutes to develop color. Measure the absorbance value at a wavelength of 528 nm using a UV spectrophotometer.

[0091] (3) Calculation formula

[0092]

[0093] In the formula:

[0094] A—Absorbance of the reaction solution without sample and containing enzyme; B—Absorbance of the reaction solution without sample and enzyme;

[0095] C—Absorbance of the reaction solution containing the sample and enzyme; D—Absorbance of the reaction solution containing the sample and without the enzyme.

[0096] 3. Experimental Results

[0097] Table 3 Results of Hyaluronidase Inhibition Rate

[0098]

[0099]

[0100] Note: Hyaluronidase inhibition rate is rounded to two decimal places.

[0101] The data in Table 3 shows that:

[0102] The solution in Example 1 of this invention contains Ophiopogon japonicus root extract, dandelion extract and oregano leaf extract, and has an extremely high hyaluronidase inhibition rate of 61.07%, which is only about 7% different from the positive control group. Examples 1 to 3 are all within the dosage range of the technical solution of this invention and have a high hyaluronidase inhibition rate.

[0103] Comparative Examples 1-3 show the use of the three components individually, with the Ophiopogon japonicus root extract exhibiting a higher hyaluronidase inhibition rate. Comparing the data from Example 1 with those from Comparative Examples 1-3, it can be seen that this invention, by combining Ophiopogon japonicus root extract, dandelion extract, and oregano leaf extract, produces a synergistic effect in increasing the hyaluronidase inhibition rate of the composition.

[0104] Comparative Examples 4-6 involved combinations of the three components in pairs. Analysis of their data revealed that the amount of Ophiopogon japonicus root in Comparative Examples 4 and 5 was significantly higher than that in Example 1. Theoretically, their hyaluronidase inhibition rate should be higher than that in Example 1. However, in reality, their hyaluronidase inhibition rate was significantly lower than that in Example 1. This indicates that by combining Ophiopogon japonicus root extract, dandelion extract, and oregano leaf extract, this study achieved a synergistic effect in increasing the hyaluronidase inhibition rate of the composition.

[0105] Comparative Example 6 used a combination of dandelion extract and oregano leaf extract. In the absence of Ophiopogon japonicus root extract, the effect it showed was simply a simple additive effect.

[0106] Macrophage detection of PGE2 inflammatory factor content

[0107] 1. Experimental Objective and Principle

[0108] Macrophages can be used as a cell model to study the inhibition of PGE2 levels by cosmetics. Through stimulation with bacterial lipopolysaccharide (LPS), the PGE2 levels in the negative control and experimental sample groups were measured to evaluate the efficacy of the experimental samples in inhibiting PGE2 levels. PGE2 levels were determined using enzyme-linked immunosorbent assay (ELISA).

[0109] This experiment followed laboratory methods, comparing the results of cell PGE2 content determination between the test sample and the negative control. If the cell PGE2 content of the test sample was lower than that of the negative control, the test sample could be considered to have a certain soothing effect.

[0110] 2. Experimental Materials and Methods

[0111] 2.1 Instruments and Equipment

[0112] RT-6100 enzyme-linked immunosorbent assay (ELISA) analyzer.

[0113] 2.2 Cells used in the experiment

[0114] Macrophages: RAW264.7 cells.

[0115] 2.3 Test Methods

[0116] (1) Sample preparation

[0117] Sample group: Dilute the compositions of Examples 1-3 and Comparative Examples 1-6 with pure water to a 0.5% solution, filter with a 0.22 μm filter, and collect the filtrate as a sample.

[0118] Negative controls: basal culture medium (cell viability assay); basal culture medium containing LPS (PGE2 content assay).

[0119] (2) Experimental Operation Procedures

[0120] PGE2 content determination: RAW264.7 cells were seeded into 96-well plates and cultured for 24 h. The culture medium was then replaced with basal medium containing different concentrations of the experimental samples. After 24 h, the cell supernatant was collected and centrifuged. The supernatant was then aspirated, and the PGE2 content in the cell culture supernatant was determined using an ELISA kit to determine the effect of the experimental samples on the PGE2 content of macrophages.

[0121] (3) Calculation formula

[0122]

[0123] 3. Test Results

[0124] Table 4. Relative content data of PGE2

[0125] sample 0.5wt% solution cell viability / % PGE2 relative content / % Example 1 97.43 51.35 Example 2 98.43 52.73 Example 3 95.45 55.36 Comparative Example 1 97.31 65.75 Comparative Example 2 95.79 79.30 Comparative Example 3 97.76 72.08 Comparative Example 4 99.07 64.64 Comparative Example 5 95.73 66.89 Comparative Example 6 95.96 71.48 negative control 100.00 100.00

[0126] The solution in Example 1 of this invention contains Ophiopogon japonicus root extract, dandelion extract and oregano leaf extract, which has a very high inhibitory effect on PGE2 inflammatory factors, and the relative content of cellular PGE2 inflammatory factors is 51.35%. Examples 1 to 3 are all within the dosage range of the technical solution of this invention and have a high effect on inhibiting the release of PGE2 inflammatory factors.

[0127] Comparative Examples 1-3 show the use of the three components individually, with the Ophiopogon japonicus root extract showing a higher effect in inhibiting the release of PGE2 inflammatory factors. Comparing the data of Example 1 with those of Comparative Examples 1-3, it can be seen that this case, by combining Ophiopogon japonicus root extract, dandelion extract, and oregano leaf extract, produced a synergistic effect in inhibiting the release of PGE2 inflammatory factors.

[0128] Comparative Examples 4-6 involved combinations of the three components in pairs. Analysis of their data revealed that the dosage of Ophiopogon japonicus root in Comparative Examples 4 and 5 was significantly higher than that in Example 1. Theoretically, the relative content of cellular PGE2 inflammatory factors should be lower than that in Example 1. However, in reality, the relative content of cellular PGE2 inflammatory factors was significantly higher than that in Example 1. This indicates that the combination of Ophiopogon japonicus root extract, dandelion extract, and oregano leaf extract in this study produced a synergistic inhibitory effect on the release of PGE2 inflammatory factors.

[0129] Comparative Example 6 used a combination of dandelion extract and oregano leaf extract. In the absence of Ophiopogon japonicus root extract, the effect it showed was simply a simple additive effect.

[0130] Application examples

[0131] This invention discloses a skincare product containing the soothing composition of Example 1, the formulation of which is shown in Table 5; the preparation method is as follows:

[0132] Step 1: Dissolve phase B in advance and stir until homogeneous; mix phase C in advance and stir until homogeneous, then set aside.

[0133] Step 2: Add the pure water from phase A to the pot, turn on the homogenizer, and add the remaining raw materials of phase A in sequence while in the homogenizer state. Homogenize at medium speed for 30-60 seconds, stirring until the temperature reaches 80-85℃. Homogenize at medium speed for 1-2 minutes until there are no gel particles. Keep warm for 30 minutes, then stir and cool down.

[0134] Step 3: Cool down to 50℃, add the pre-prepared phases B and C in sequence, and stir evenly.

[0135] Step 4: Cool down to 45℃, add phase D, and stir well.

[0136] Table 5 Skincare Product Formula Table

[0137]

[0138] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A soothing composition, characterized in that, The soothing composition is obtained by combining extracts of Ophiopogon japonicus root, dandelion, and oregano leaf; the extraction method of the extracts is as follows: Step 1: Add water to the plant combination of Ophiopogon japonicus root, dandelion and oregano leaf, and extract by circulation to obtain an extract. Then concentrate the extract to obtain a first concentrate. Add ethanol of the same mass as the first concentrate to obtain an alcohol precipitate. The weight ratio of Ophiopogon japonicus root, dandelion and oregano leaf in the plant combination is 2~10:2~10:1~8. Step 2: After centrifugation and filtration, the filtrate is concentrated until alcohol-free to obtain a second concentrate. Butylene glycol and water are added to the second concentrate, and the mixture is stirred and extracted. Ultrafiltration is then performed to obtain an ultrafiltrate, wherein the weight ratio of concentrate, butylene glycol, and water is 3~5:3:2~4. Step 3: Add preservatives to the ultrafiltrate, sterilize, and obtain the soothing composition.

2. Use of the soothing composition according to claim 1 in the preparation of skin care products.

3. The use according to claim 2, characterized in that, The skincare product in question is a soothing product.

4. A skincare product, characterized in that, Contains the soothing composition according to claim 1.

5. The skincare product according to claim 4, characterized in that, The content of soothing composition in skin care products is 0.1~30wt%.

6. The skincare product according to claim 4, characterized in that, The skincare products are available in the following forms: lotion, cream, spray, toner, mask, and gel.

7. The skincare product according to claim 4, characterized in that, The skincare product is in the form of an essence.

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